##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938786_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2764304 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.050449950511954 33.0 31.0 34.0 30.0 34.0 2 32.23691316150467 34.0 31.0 34.0 30.0 34.0 3 32.3111665721281 34.0 31.0 34.0 30.0 34.0 4 35.953356794332315 37.0 35.0 37.0 35.0 37.0 5 35.86049363601109 37.0 35.0 37.0 35.0 37.0 6 35.88504014030295 37.0 35.0 37.0 35.0 37.0 7 35.79851890385428 37.0 35.0 37.0 33.0 37.0 8 35.79104215744723 37.0 35.0 37.0 33.0 37.0 9 37.42941622918463 39.0 37.0 39.0 34.0 39.0 10-11 37.3599251384797 39.0 37.0 39.0 34.0 39.0 12-13 37.284642535697955 39.0 37.0 39.0 33.5 39.0 14-15 38.486723602035084 40.0 38.0 41.0 33.5 41.0 16-17 38.422934850870234 40.0 38.0 41.0 33.0 41.0 18-19 38.5699516044545 40.0 38.0 41.0 34.0 41.0 20-21 38.57407850221973 40.0 38.0 41.0 34.0 41.0 22-23 38.481523016281855 40.0 38.0 41.0 34.0 41.0 24-25 38.39772615457635 40.0 38.0 41.0 34.0 41.0 26-27 38.21682437966302 40.0 38.0 41.0 33.0 41.0 28-29 38.041777966533346 40.0 37.0 41.0 33.0 41.0 30-31 37.78123480630205 40.0 37.0 41.0 33.0 41.0 32-33 37.58003009075702 40.0 36.0 41.0 32.5 41.0 34-35 37.51248813444542 40.0 36.0 41.0 32.0 41.0 36-37 37.37355334290295 40.0 36.0 41.0 32.0 41.0 38-39 37.23001070070441 40.0 36.0 41.0 31.5 41.0 40-41 37.03504770097645 40.0 36.0 41.0 31.0 41.0 42-43 36.80651531090648 40.0 35.5 41.0 31.0 41.0 44-45 36.50961779167559 39.5 35.0 41.0 30.0 41.0 46-47 36.108101352094415 39.0 35.0 41.0 29.5 41.0 48-49 35.732574818109725 39.0 35.0 41.0 27.5 41.0 50-51 35.47606178625796 39.0 35.0 41.0 27.0 41.0 52-53 35.24710740931533 39.0 35.0 40.0 26.5 41.0 54-55 34.967408251769704 38.0 34.5 40.0 26.0 41.0 56-57 34.69478917658839 38.0 34.0 40.0 25.5 41.0 58-59 34.39154286214541 38.0 34.0 40.0 25.0 41.0 60-61 34.041386005301874 37.5 33.5 40.0 23.5 41.0 62-63 33.681215597126794 37.0 33.0 40.0 22.5 41.0 64-65 33.28143359051681 36.5 33.0 39.0 22.0 41.0 66-67 32.88253878734032 36.0 32.0 39.0 21.0 40.0 68-69 32.56043311444762 35.5 32.0 39.0 20.0 40.0 70-71 32.15086618548466 35.0 32.0 38.0 18.5 40.0 72-73 31.681107251590277 35.0 31.0 37.5 17.0 39.5 74-75 31.195249147705898 35.0 31.0 37.0 12.5 39.0 76-77 29.241268869125825 33.0 28.5 35.0 8.0 37.5 78-79 30.180634799935174 34.0 30.0 36.0 7.0 38.0 80-81 30.234249561553288 34.0 30.0 35.5 6.5 37.0 82-83 29.91566177236657 34.0 30.0 35.0 2.0 37.0 84-85 29.52736674403394 34.0 30.0 35.0 2.0 36.5 86-87 28.958147150241075 34.0 29.0 35.0 2.0 36.0 88-89 28.373954348002243 34.0 29.0 35.0 2.0 35.5 90-91 27.87404370141634 33.5 27.0 35.0 2.0 35.0 92-93 27.58848267050223 33.0 27.0 35.0 2.0 35.0 94-95 27.263671434111444 33.0 26.5 35.0 2.0 35.0 96-97 26.695000079586038 33.0 25.5 35.0 2.0 35.0 98-99 25.920985354722202 33.0 24.5 35.0 2.0 35.0 100-101 22.505481126533116 28.5 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.026366031343528107 1101 2 0.10717351104248962 1101 3 0.045155356245977885 1101 4 0.08158201875455262 1101 5 -0.17520179350034226 1101 6 -0.016976000725236418 1101 7 0.13138435740414423 1101 8 0.15745435669010988 1101 9 0.18485413017750574 1101 10-11 0.18980128583287836 1101 12-13 0.11905123730085876 1101 14-15 0.2563871158129061 1101 16-17 0.26514721287272636 1101 18-19 0.23776447231056608 1101 20-21 0.21963098005143422 1101 22-23 0.08688971846315496 1101 24-25 0.23845361168188361 1101 26-27 0.18986616305188875 1101 28-29 0.2946008003098157 1101 30-31 0.2613279771309607 1101 32-33 0.21851305599226123 1101 34-35 0.3007690298462862 1101 36-37 0.26123730858717664 1101 38-39 0.29509155235566453 1101 40-41 0.2741002745016772 1101 42-43 0.27529400959606676 1101 44-45 0.2535866200479191 1101 46-47 0.1782155573979196 1101 48-49 0.10008071767021676 1101 50-51 0.2344466092381836 1101 52-53 0.07212800466813718 1101 54-55 0.24203929025935622 1101 56-57 0.2159087025747013 1101 58-59 0.2138382856407759 1101 60-61 0.10082135236754652 1101 62-63 0.04040809426007286 1101 64-65 -0.004735367652060063 1101 66-67 0.12782511852159217 1101 68-69 0.22520062401596164 1101 70-71 0.1700509302141029 1101 72-73 0.07272420196567708 1101 74-75 0.010990796287341453 1101 76-77 -0.052769868386516094 1101 78-79 -0.33644586677830546 1101 80-81 -0.09179964181745959 1101 82-83 0.026406079795634696 1101 84-85 0.07341425961138981 1101 86-87 -0.0012753235940401453 1101 88-89 -0.004350681291228398 1101 90-91 0.13204300050982454 1101 92-93 0.21769283133079043 1101 94-95 0.2778591596219755 1101 96-97 0.24104357394118736 1101 98-99 0.18892840863783888 1101 100-101 0.7256743689863612 1102 1 0.20861880414251033 1102 2 0.1000191813178759 1102 3 0.11952157362153315 1102 4 0.12185300423627154 1102 5 -0.003974816385287738 1102 6 -0.03405660238463071 1102 7 0.1105903641737811 1102 8 0.13360125991717098 1102 9 0.16996886333880212 1102 10-11 0.16949044047333217 1102 12-13 0.1707056492755612 1102 14-15 0.1986736854016229 1102 16-17 0.23464178322453932 1102 18-19 0.23351345646485555 1102 20-21 0.18517547563563141 1102 22-23 0.10084067570567612 1102 24-25 0.17934057321512142 1102 26-27 0.25052553173676984 1102 28-29 0.15205982662869388 1102 30-31 0.14971453598146667 1102 32-33 0.13018709946371132 1102 34-35 0.1596748580646974 1102 36-37 0.2268120767732924 1102 38-39 0.25208061080920174 1102 40-41 0.21956675245009194 1102 42-43 0.2244698094496158 1102 44-45 0.09701429537483364 1102 46-47 0.0531067473853426 1102 48-49 0.05518302192928104 1102 50-51 0.02432426508782015 1102 52-53 -0.10562807298508403 1102 54-55 -0.013530654301803224 1102 56-57 0.016933030323023956 1102 58-59 0.12473216703386925 1102 60-61 0.09702978037416443 1102 62-63 -0.041170142138099663 1102 64-65 0.1041990196247582 1102 66-67 0.2644972770951881 1102 68-69 0.17287407460136706 1102 70-71 0.09095676071723346 1102 72-73 0.007880788427080176 1102 74-75 0.012424960803510743 1102 76-77 0.02664680427755428 1102 78-79 0.0625180391413025 1102 80-81 0.12702964526847538 1102 82-83 0.08103620286232882 1102 84-85 0.24726345807285455 1102 86-87 0.3270811654238628 1102 88-89 0.2974493818814068 1102 90-91 0.23454431708241685 1102 92-93 0.34474451408762974 1102 94-95 0.39658237836494337 1102 96-97 0.5244310409697519 1102 98-99 0.37186766980357433 1102 100-101 1.1048692662822752 1103 1 0.1679386746758027 1103 2 0.11330592501128223 1103 3 0.0847868882662226 1103 4 0.008014516994634846 1103 5 -0.022360850162264967 1103 6 0.0654733938520593 1103 7 0.08675454833946361 1103 8 0.11341094958243758 1103 9 0.19099393636491158 1103 10-11 0.20031366040684162 1103 12-13 0.22861635592322926 1103 14-15 0.11940575102739359 1103 16-17 -0.002146385011052132 1103 18-19 -0.04873204822899879 1103 20-21 0.054319790393265066 1103 22-23 0.125503683160197 1103 24-25 0.07312657140111867 1103 26-27 0.23238982952408094 1103 28-29 0.02538316429583176 1103 30-31 0.17968625351577572 1103 32-33 0.34927806248035154 1103 34-35 0.19032658281722803 1103 36-37 0.23232221902191696 1103 38-39 0.11991374106649033 1103 40-41 0.33169987327480044 1103 42-43 0.3804850671723585 1103 44-45 0.35979332581373313 1103 46-47 0.46697310172564244 1103 48-49 0.38667362136779815 1103 50-51 0.3730695441081835 1103 52-53 0.3174061539522839 1103 54-55 0.3830068378510276 1103 56-57 0.200179625112213 1103 58-59 0.2990323256929557 1103 60-61 0.32445892383040587 1103 62-63 0.24312163555357813 1103 64-65 0.3435022421366014 1103 66-67 0.40691058450999407 1103 68-69 0.3588464633910107 1103 70-71 0.3109183274594258 1103 72-73 0.4597303733309559 1103 74-75 0.5889237585313012 1103 76-77 0.5792749870273752 1103 78-79 0.3123505378740141 1103 80-81 0.4866702513820407 1103 82-83 0.44265487287378846 1103 84-85 0.3436273573717763 1103 86-87 0.4581571757833096 1103 88-89 0.3654105813224504 1103 90-91 0.47086363458021907 1103 92-93 0.47221530538125833 1103 94-95 0.516871835002366 1103 96-97 0.5004907582451175 1103 98-99 0.7472057459152062 1103 100-101 1.3019788396864485 1104 1 0.1053396990537081 1104 2 0.09302440414013802 1104 3 0.0968519701484638 1104 4 0.03661391267388581 1104 5 -0.09677647937126466 1104 6 -0.007628524509861734 1104 7 -0.04785284824485814 1104 8 -0.002048061950972624 1104 9 0.09204542804950933 1104 10-11 0.09444081550508798 1104 12-13 -0.029900467971252453 1104 14-15 0.06617038882168913 1104 16-17 0.20128425146912576 1104 18-19 0.19345195717284014 1104 20-21 0.14667703496148476 1104 22-23 0.11843476268229125 1104 24-25 0.0818900343566753 1104 26-27 0.07805536823912007 1104 28-29 0.07638012562552632 1104 30-31 -0.07207029281065758 1104 32-33 0.04896300585384239 1104 34-35 0.21283184660678245 1104 36-37 0.23421151276711072 1104 38-39 0.327829506119663 1104 40-41 0.32495624934871614 1104 42-43 0.335302084937247 1104 44-45 0.39237638275625386 1104 46-47 0.45412653737198383 1104 48-49 0.30977701240714595 1104 50-51 0.2711754056671438 1104 52-53 0.3406609692633964 1104 54-55 0.32661038042475354 1104 56-57 0.3129098479489514 1104 58-59 0.41297014402181276 1104 60-61 0.42934301035336375 1104 62-63 0.4124603400764073 1104 64-65 0.291287569259552 1104 66-67 0.2710428788395731 1104 68-69 0.365277659488612 1104 70-71 0.43983155482788305 1104 72-73 0.5252009323070013 1104 74-75 0.4288335370819034 1104 76-77 0.36721026299198556 1104 78-79 0.31059013781224465 1104 80-81 0.3808181891243976 1104 82-83 0.49285507442414556 1104 84-85 0.46281561482001266 1104 86-87 0.5077215798210339 1104 88-89 0.5684582528542776 1104 90-91 0.5669852903981614 1104 92-93 0.5283345691306316 1104 94-95 0.5755997334861647 1104 96-97 0.6286012387811404 1104 98-99 0.8169886347247548 1104 100-101 1.5002767136081943 1105 1 0.05179955703612649 1105 2 0.13846610350019262 1105 3 0.1042518912409065 1105 4 0.06277387918758848 1105 5 -0.002812907738345416 1105 6 -0.008500274080667225 1105 7 0.037365179969000906 1105 8 0.15328657261807166 1105 9 0.13299134645311028 1105 10-11 0.11830741418054913 1105 12-13 0.12044875274312261 1105 14-15 0.11683684250383664 1105 16-17 0.14607573810732077 1105 18-19 0.16223752601113972 1105 20-21 0.08686956513481903 1105 22-23 0.0265885928910663 1105 24-25 0.11557633700323322 1105 26-27 0.09492319487007705 1105 28-29 0.23627872380092896 1105 30-31 0.21166061905248057 1105 32-33 0.12714206030406672 1105 34-35 0.11810484299183344 1105 36-37 0.20671634164802555 1105 38-39 0.21017964252503418 1105 40-41 0.1658990157238378 1105 42-43 0.17912975873221626 1105 44-45 0.1384134415290248 1105 46-47 -0.09592660636238293 1105 48-49 -0.07447676221373456 1105 50-51 -0.07479912006609624 1105 52-53 -0.0283193575378462 1105 54-55 -0.03415510571821301 1105 56-57 -0.02004192521398096 1105 58-59 -0.07134163593667608 1105 60-61 8.056397167592877E-4 1105 62-63 0.0048810872620848045 1105 64-65 0.0568550505831098 1105 66-67 0.06890729940992912 1105 68-69 -0.03293319038107256 1105 70-71 -0.09493264806551593 1105 72-73 -0.04690652728893596 1105 74-75 -0.040735898499313805 1105 76-77 0.03686018214779807 1105 78-79 -0.21860305508842615 1105 80-81 -0.10847746874268083 1105 82-83 -0.07243111506993216 1105 84-85 -0.06683742698095685 1105 86-87 0.07135395606852413 1105 88-89 -0.035304120527001714 1105 90-91 -0.1461699675726642 1105 92-93 0.0042922715796755995 1105 94-95 0.21581384704093054 1105 96-97 0.284286402957747 1105 98-99 0.3880983971713263 1105 100-101 0.8602102512179073 1106 1 0.18760913754076114 1106 2 0.11581853574255518 1106 3 0.11383527469653387 1106 4 0.08334304230456979 1106 5 0.006363328431753246 1106 6 0.04032424235482068 1106 7 0.04858357850244488 1106 8 0.030941640727853326 1106 9 0.03767634194467462 1106 10-11 0.002742358521665267 1106 12-13 0.0659771240203284 1106 14-15 0.08686981269469385 1106 16-17 0.06930277104204663 1106 18-19 -0.06036472604798604 1106 20-21 -0.060301519491162026 1106 22-23 0.02858480981421252 1106 24-25 0.03613847014656102 1106 26-27 0.14227932074027194 1106 28-29 0.09908634619444001 1106 30-31 0.1495307529484151 1106 32-33 0.09910814472955565 1106 34-35 0.08608588339708945 1106 36-37 0.043273888215502154 1106 38-39 0.18412093106393712 1106 40-41 0.4510749732653707 1106 42-43 0.3228873863178876 1106 44-45 0.30175012406004953 1106 46-47 0.3825122588276457 1106 48-49 0.4426488062245397 1106 50-51 0.45936590927215804 1106 52-53 0.46831582975504915 1106 54-55 0.4990049715369622 1106 56-57 0.4701103283359558 1106 58-59 0.509415796814622 1106 60-61 0.5116772947601902 1106 62-63 0.5025542559475227 1106 64-65 0.5195514014741605 1106 66-67 0.46220232388503035 1106 68-69 0.3575828739440823 1106 70-71 0.3812120295632795 1106 72-73 0.470490087116886 1106 74-75 0.502286961895436 1106 76-77 0.31137647626441733 1106 78-79 0.20375872560518715 1106 80-81 0.22646697518769088 1106 82-83 0.3203058811495616 1106 84-85 0.41221035987538457 1106 86-87 0.3685461563191872 1106 88-89 0.5728958489369518 1106 90-91 0.6144442002275383 1106 92-93 0.8205052414826497 1106 94-95 0.8083536184421227 1106 96-97 0.732284197062544 1106 98-99 0.8700785955219708 1106 100-101 1.4454919282534604 1107 1 0.11271401424421867 1107 2 0.01826877940061422 1107 3 0.034222091353335315 1107 4 -0.018034903478351794 1107 5 -0.0643870776650104 1107 6 -0.012807636620749463 1107 7 0.05425400504321232 1107 8 0.04271136235547601 1107 9 -0.007946289157530373 1107 10-11 0.009245979234876245 1107 12-13 0.08351266065000118 1107 14-15 0.07700691146855121 1107 16-17 -0.0010842986345451777 1107 18-19 0.042210129857245704 1107 20-21 0.018651218942039804 1107 22-23 -0.004517579704703678 1107 24-25 -0.025547059145544893 1107 26-27 0.0385115397744471 1107 28-29 0.04556399442608239 1107 30-31 0.0471676113534798 1107 32-33 -0.030537804676612268 1107 34-35 0.07081468413388592 1107 36-37 0.02952713319432121 1107 38-39 0.019198835857658025 1107 40-41 -0.06744974423803285 1107 42-43 -0.11592528035890126 1107 44-45 0.05022827327087498 1107 46-47 -0.12044699739537634 1107 48-49 -0.09717948189415893 1107 50-51 -0.1917771076346213 1107 52-53 -0.19667641518835666 1107 54-55 -0.12824570276077907 1107 56-57 -0.17645024713225155 1107 58-59 -0.03380390533048683 1107 60-61 -0.006733943366853623 1107 62-63 -0.09754783753822949 1107 64-65 -0.09452453229538094 1107 66-67 -0.0683027542221808 1107 68-69 -0.014277909506404285 1107 70-71 -0.057540038873518995 1107 72-73 0.020943182307988195 1107 74-75 0.19594559012333335 1107 76-77 0.05424808643176604 1107 78-79 0.05326236402978424 1107 80-81 0.17919528759628633 1107 82-83 0.16683191941627484 1107 84-85 -0.049216456680209575 1107 86-87 0.12764209130321902 1107 88-89 -0.031378983690458995 1107 90-91 0.06529757272833336 1107 92-93 0.39628732565727276 1107 94-95 0.5695028090318139 1107 96-97 0.7165030364101561 1107 98-99 0.7494936011137092 1107 100-101 1.447433349111403 1108 1 0.20468931662094292 1108 2 0.010690330614352206 1108 3 0.005009877871138713 1108 4 0.05968817864970788 1108 5 -0.02483569476726899 1108 6 0.00514719717777723 1108 7 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97.0 6 235.0 7 637.0 8 1309.0 9 2545.0 10 4042.0 11 6457.0 12 9391.0 13 13053.0 14 14283.0 15 18512.0 16 23257.0 17 32568.0 18 30773.0 19 19194.0 20 16939.0 21 17604.0 22 18855.0 23 20919.0 24 23892.0 25 28236.0 26 33152.0 27 39991.0 28 48695.0 29 58823.0 30 71276.0 31 86310.0 32 105940.0 33 133217.0 34 175623.0 35 244216.0 36 364011.0 37 566070.0 38 486947.0 39 47180.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64052617475752 20.223962939474603 14.870731619762664 22.264779266005213 2 15.765849197483345 22.165941227882318 39.186753700027204 22.881455874607134 3 18.15990571225162 26.86140887543483 31.507894934855212 23.47079047745834 4 12.820731728492959 18.164355295220787 37.636019772065595 31.378893204220663 5 12.820593541775674 38.45808971517029 34.21017779331337 14.51113894974066 6 29.472471712358722 37.31870386763877 18.376394122986348 14.832430297016163 7 26.42401125201859 34.55354403857173 21.922878236257663 17.099566473152013 8 25.885720239163273 34.60310443424457 20.939158645358834 18.572016681233322 9 26.767533527426796 16.573358067708906 21.194629823637342 35.464478581226956 10-11 24.178798713889645 27.230018840185448 27.94410455579415 20.647077890130753 12-13 25.356979550729587 25.185706781887955 28.484837412961816 20.972476254420645 14-15 22.515396280582745 25.992257002124227 27.347136928499904 24.145209788793128 16-17 21.09525330663019 29.26672958980457 27.883572177168308 21.754444926396932 18-19 20.973796659122875 27.320367079742315 30.968771886160134 20.737064374974675 20-21 22.125768367010284 26.51367215762087 30.813181184124467 20.547378291244378 22-23 21.7675588502567 26.706668296974573 30.636608708738255 20.889164144030467 24-25 21.43602488516522 26.82274610457087 30.862759887457848 20.878469122806067 26-27 21.214814670111334 27.030067755596544 30.748820621403777 21.006296952888345 28-29 21.08630237484734 27.08332730408812 30.783444946720767 21.04692537434378 30-31 21.32770129479247 26.934718468012203 30.746600229207786 20.990980007987545 32-33 21.134343400725825 27.077177473968128 30.54553695975551 21.24294216555053 34-35 21.52295179766139 27.08302356012892 30.43879442238196 20.95523021982773 36-37 21.65101124502075 26.866461963955658 30.257027753541216 21.225499037482376 38-39 21.358291575462655 27.08272580907759 30.35802022467845 21.200962390781307 40-41 21.46329630406127 26.917382291975127 30.398981648287698 21.220339755675905 42-43 21.37661720286718 27.04636466941065 30.030943063388598 21.54607506433357 44-45 21.322428948028172 27.231153650103877 29.861761538291788 21.584655863576163 46-47 21.587631704086416 27.3032512647445 29.37806712637424 21.73104990479484 48-49 21.67443186360747 27.484003839006288 28.978347527020826 21.86321677036542 50-51 21.558884543611075 27.7487077667123 28.791628705024614 21.90077898465201 52-53 21.718794314952337 27.798552547042583 28.66408687322378 21.818566264781296 54-55 21.37375266974978 27.73050648553849 28.65149780921346 22.244243035498265 56-57 21.449143922990057 28.004101630577228 28.469559646293146 22.077194800139566 58-59 21.525462540914578 27.54893108408072 28.673014756740955 22.252591618263747 60-61 21.488321749080487 27.71652326985961 28.781462976636263 22.01369200442364 62-63 21.36613602321468 27.62158612053639 28.8211366011217 22.191141255127228 64-65 21.149176671130782 27.611995372370096 28.730881904997563 22.507946051501555 66-67 21.14036937843979 27.82511991902052 28.427527814085813 22.606982888453874 68-69 21.14496297252938 27.604720039330044 28.585909121987164 22.664407866153407 70-71 21.107342229245088 27.658178633424296 28.406341778317756 22.828137359012857 72-73 21.220838952589872 27.693336188783867 28.11204918127673 22.973775677349526 74-75 21.112855377746474 27.864921772987643 27.934704496934025 23.087518352331852 76-77 21.09028057427006 27.66168199772862 28.213672622629645 23.03436480537168 78-79 21.051809813206166 27.71361490612127 28.066006550445348 23.168568730227214 80-81 21.039853922310762 27.732657570273663 27.921712053670344 23.30577645374523 82-83 21.142891871535724 27.714190933933754 27.792963535611005 23.349953658919517 84-85 20.95621538007397 27.545975406467594 27.77465864825287 23.723150565205565 86-87 21.006933944823224 27.364583103221875 27.849003184320093 23.779479767634808 88-89 21.113268011560855 27.26636405518745 27.707429422824813 23.91293851042688 90-91 20.938449055965112 27.32812666839544 27.822625457889426 23.91079881775002 92-93 21.025109521794022 27.27514117549172 27.597194939749446 24.102554362964813 94-95 20.82087387105153 27.039704112942957 27.724724324222645 24.41469769178287 96-97 21.061331790809284 26.890889756739544 27.600363998053034 24.44741445439814 98-99 20.790477458340327 26.938155137785134 27.484567543945964 24.786799859928575 100-101 20.942811235710767 26.512932593006738 27.98503622377726 24.55921994750523 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2123.0 1 1487.5 2 1127.5 3 1468.0 4 1300.0 5 1254.5 6 1940.5 7 3423.5 8 5033.0 9 5708.5 10 5115.0 11 4463.0 12 4609.5 13 4897.5 14 5195.0 15 5480.0 16 5811.5 17 6259.5 18 6700.0 19 7153.5 20 7721.5 21 8568.5 22 9872.5 23 11494.5 24 13220.5 25 15681.5 26 19454.5 27 23874.5 28 27471.0 29 31287.0 30 36282.0 31 41809.5 32 47841.0 33 52846.5 34 58382.0 35 63850.5 36 69562.0 37 75320.5 38 78101.5 39 78430.0 40 79274.5 41 82617.5 42 85912.0 43 90499.5 44 95277.5 45 98041.5 46 99566.5 47 100150.0 48 102946.0 49 103849.0 50 100361.0 51 96182.5 52 91915.0 53 89268.5 54 86010.5 55 79541.5 56 71632.5 57 63185.0 58 56740.0 59 50976.5 60 43407.0 61 35457.0 62 28620.0 63 22010.0 64 15942.0 65 11819.5 66 8507.0 67 6423.5 68 4717.5 69 3234.5 70 2413.5 71 1744.5 72 1366.0 73 1158.5 74 896.5 75 705.5 76 490.0 77 272.0 78 170.5 79 123.5 80 85.5 81 60.0 82 43.5 83 32.5 84 26.5 85 17.0 86 11.0 87 12.5 88 11.0 89 7.5 90 5.0 91 3.5 92 2.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2328615087197356 2 0.0 3 0.0 4 0.0 5 0.027421007240882336 6 0.004015477313638442 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0030749150599934014 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.351405634112601E-4 26-27 0.0011033518744682205 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006692462189397404 36-37 0.01658645358831735 38-39 0.012806116838090168 40-41 0.008302270661982183 42-43 0.007054216902337804 44-45 0.003436669772933802 46-47 3.798424485874202E-4 48-49 0.0017002471508198809 50-51 1.808773564702001E-5 52-53 0.0 54-55 0.0 56-57 0.0013565801735265007 58-59 2.8940377035232016E-4 60-61 0.002279054691524521 62-63 6.692462189397403E-4 64-65 0.0013746679091735207 66-67 0.005317794280223883 68-69 1.808773564702001E-4 70-71 1.808773564702001E-5 72-73 0.0 74-75 3.436669772933802E-4 76-77 0.060286422911517694 78-79 0.14039700409216932 80-81 7.777726328218604E-4 82-83 6.511584832927204E-4 84-85 0.0 86-87 0.1071155705016525 88-89 0.35585811111947163 90-91 0.3946743918179766 92-93 5.064565981165603E-4 94-95 2.170528277642401E-4 96-97 1.2661414952914006E-4 98-99 0.0 100-101 0.0010310009318801406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2764304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.73779094811958 #Duplication Level Percentage of deduplicated Percentage of total 1 77.60204848594525 40.9256061019762 2 12.386063759771563 13.064272824658252 3 3.9857844212856888 6.306043967221093 4 1.758574135308331 3.7097326045864367 5 1.0218072012278567 2.694392728381893 6 0.641006155202677 2.0283149165722008 7 0.42835663653347883 1.5813407924119574 8 0.31014354077565803 1.3085028173869002 9 0.2567974212115216 1.2188635844282492 >10 1.4238990378102612 14.082992572923434 >50 0.11737032050897987 4.276639511939929 >100 0.06485375264553117 6.004422342320493 >500 0.0022654030940908137 0.7191511683405059 >1k 8.237829432741876E-4 0.7181012597444388 >5k 6.864857860618229E-5 0.3206565594936473 >10k+ 1.3729715721236458E-4 1.0409662476144115 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15726 0.5688954615700733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13027 0.4712578645474593 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8857 0.32040614925131244 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3010 0.10888816859506045 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.235094258808004E-5 0.0 0.0 0.0 0.0 6 7.235094258808004E-5 0.0 0.0 0.0 0.0 7 7.235094258808004E-5 0.0 0.0 0.0 0.0 8 7.235094258808004E-5 0.0 0.0 0.0 0.0 9 7.235094258808004E-5 0.0 0.0 0.0 0.0 10-11 7.235094258808004E-5 0.0 0.0 0.0 0.0 12-13 7.235094258808004E-5 0.0 0.0 0.0 0.0 14-15 7.235094258808004E-5 0.0 0.0 0.0 0.0 16-17 7.235094258808004E-5 0.0 0.0 0.0 5.426320694106003E-5 18-19 7.235094258808004E-5 0.0 0.0 7.235094258808004E-5 7.235094258808004E-5 20-21 7.235094258808004E-5 0.0 0.0 2.170528277642401E-4 7.235094258808004E-5 22-23 7.235094258808004E-5 0.0 0.0 6.330707476457003E-4 7.235094258808004E-5 24-25 1.0852641388212006E-4 0.0 0.0 0.0015917207369377608 9.043867823510006E-5 26-27 1.2661414952914006E-4 0.0 0.0 0.003527108451168902 1.0852641388212006E-4 28-29 1.4470188517616008E-4 0.0 0.0 0.010726027238682866 1.0852641388212006E-4 30-31 1.4470188517616008E-4 0.0 0.0 0.03319099491228172 1.0852641388212006E-4 32-33 1.808773564702001E-4 0.0 0.0 0.07323724163478403 1.0852641388212006E-4 34-35 1.808773564702001E-4 0.0 0.0 0.13553140320312093 1.0852641388212006E-4 36-37 2.1705282776424012E-4 0.0 0.0 0.23246357853550115 1.0852641388212006E-4 38-39 2.1705282776424012E-4 0.0 0.0 0.3785039561495407 1.0852641388212006E-4 40-41 2.1705282776424012E-4 0.0 0.0 0.5490351278296454 1.0852641388212006E-4 42-43 2.1705282776424012E-4 0.0 0.0 0.7237988296511526 1.0852641388212006E-4 44-45 2.1705282776424012E-4 0.0 0.0 0.9265623462542469 1.0852641388212006E-4 46-47 2.1705282776424012E-4 0.0 0.0 1.166224843577262 1.0852641388212006E-4 48-49 2.5322829905828013E-4 0.0 0.0 1.4261817802962335 1.0852641388212006E-4 50-51 2.5322829905828013E-4 0.0 0.0 1.7150248308434963 1.0852641388212006E-4 52-53 2.5322829905828013E-4 0.0 0.0 2.0062735502318123 1.0852641388212006E-4 54-55 2.8940377035232016E-4 3.617547129404002E-5 0.0 2.315591917531502 1.0852641388212006E-4 56-57 2.8940377035232016E-4 3.617547129404002E-5 0.0 2.6671813230382764 1.0852641388212006E-4 58-59 2.8940377035232016E-4 3.617547129404002E-5 0.0 3.05453018191921 1.0852641388212006E-4 60-61 2.8940377035232016E-4 3.617547129404002E-5 0.0 3.4806772337630014 1.0852641388212006E-4 62-63 2.8940377035232016E-4 3.617547129404002E-5 0.0 3.9146200996706586 1.0852641388212006E-4 64-65 2.8940377035232016E-4 3.617547129404002E-5 0.0 4.358692097540647 1.0852641388212006E-4 66-67 3.436669772933802E-4 3.617547129404002E-5 0.0 4.85577924859205 1.0852641388212006E-4 68-69 3.979301842344402E-4 3.617547129404002E-5 0.0 5.398682634037357 1.0852641388212006E-4 70-71 4.5219339117550023E-4 3.617547129404002E-5 0.0 5.9983091584717165 1.0852641388212006E-4 72-73 4.702811268225202E-4 3.617547129404002E-5 0.0 6.6187908421070905 1.0852641388212006E-4 74-75 4.702811268225202E-4 3.617547129404002E-5 0.0 7.25144557183291 1.0852641388212006E-4 76-77 4.702811268225202E-4 3.617547129404002E-5 0.0 7.913456696513842 1.0852641388212006E-4 78-79 4.702811268225202E-4 3.617547129404002E-5 0.0 8.624340882913023 1.0852641388212006E-4 80-81 5.607198050576203E-4 3.617547129404002E-5 0.0 9.392472752635022 1.0852641388212006E-4 82-83 5.788075407046403E-4 3.617547129404002E-5 0.0 10.181911974949209 1.0852641388212006E-4 84-85 5.788075407046403E-4 3.617547129404002E-5 0.0 10.979653467925381 1.0852641388212006E-4 86-87 5.788075407046403E-4 3.617547129404002E-5 0.0 11.804219072866083 1.0852641388212006E-4 88-89 5.788075407046403E-4 3.617547129404002E-5 0.0 12.659913670855303 1.0852641388212006E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8900 0.0 48.543842 1 GTATCAA 15245 0.0 39.32615 1 ATCAACG 19360 0.0 30.5969 3 TATCAAC 19455 0.0 30.471891 2 TCAACGC 19590 0.0 30.261902 4 CAACGCA 19855 0.0 29.88191 5 AACGCAG 20400 0.0 29.095276 6 ACGCAGA 23125 0.0 25.440605 7 GTACATG 14940 0.0 25.383183 1 CGCAGAG 23365 0.0 24.996532 8 TACATGG 15240 0.0 24.355106 2 TAGCGCG 100 0.0016614611 23.73221 4 GCAGAGT 24315 0.0 23.571117 9 ACATGGG 15195 0.0 23.490126 3 GAGTACT 13820 0.0 22.624584 12-13 CATGGGG 5705 0.0 20.882683 4 AGTACTT 14300 0.0 20.82001 12-13 GTACTTT 15395 0.0 20.356184 14-15 AGAGTAC 22155 0.0 20.355585 10-11 CAGAGTA 23940 0.0 20.116161 10-11 >>END_MODULE