FastQCFastQC Report
Thu 26 May 2016
SRR938785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012835
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66400.6555855593457967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52860.5219013955876327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35940.35484555727240863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28470.2810921818460065No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.1545167771650861No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.11512240394536129No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.11127182611185434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.046.9708861
GTATCAA53150.037.107251
ATCAACG67000.029.3454933
TATCAAC67000.029.1333582
TCAACGC68000.029.0532884
CAACGCA68800.028.6465975
AACGCAG70850.027.731676
GTACATG36850.024.702021
ACGCAGA79350.024.5869037
CGCAGAG79900.024.2991268
TACATGG37800.023.6885322
ACATGGG36900.022.982373
GCAGAGT84150.021.9464389
GAGTACT50700.021.90331612-13
GTCCTAC4400.021.5499381
CATGGGA22850.021.3559634
ACCGTGG1805.307693E-621.046078
GGACGGC1605.116274E-520.7131356
AGTACTT52950.020.0782312-13
GCCGTAT1552.5465852E-1019.94320394-95
Produced by FastQC (version 0.11.5)