FastQCFastQC Report
Thu 26 May 2016
SRR938785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012835
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61130.6035533922109722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52860.5219013955876327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35530.3507975139089783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30020.29639576041507254No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT21240.20970839277868555No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.14572956108349336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.12716780127069066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.11028449846223719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33300.047.905521
GTATCAA53100.034.167681
TATCAAC67200.026.7627052
ATCAACG68400.026.1544343
TCAACGC68850.026.0524124
CAACGCA69450.025.759015
AACGCAG70850.025.1830356
GTGGACG1000.00166314523.7263034
CGGTATC1050.002208819422.596482
ACGCAGA79950.022.1979667
CGCAGAG81150.021.811248
GAGTACT51450.021.58407612-13
GTACATG39500.020.8561441
GGACCGT1150.00374457120.6315676
GCAGAGT84950.020.2769839
AGTACTT52700.020.12657512-13
TACATGG40050.019.7867282
AGAGTAC74350.019.5318510-11
GTACTTT56700.019.46000114-15
ACATGGG38400.019.4011963
Produced by FastQC (version 0.11.5)