##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938785_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012835 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91747026909615 31.0 31.0 34.0 30.0 34.0 2 32.13661060291163 33.0 31.0 34.0 30.0 34.0 3 32.216600927100664 34.0 31.0 34.0 30.0 34.0 4 35.88199065000716 37.0 35.0 37.0 35.0 37.0 5 35.78445452615678 37.0 35.0 37.0 35.0 37.0 6 35.81022476513944 37.0 35.0 37.0 33.0 37.0 7 35.7430055240982 37.0 35.0 37.0 33.0 37.0 8 35.71528531300755 37.0 35.0 37.0 33.0 37.0 9 37.350058992827066 39.0 37.0 39.0 34.0 39.0 10-11 37.28179713378783 39.0 37.0 39.0 33.0 39.0 12-13 37.216016922795916 39.0 37.0 39.0 33.0 39.0 14-15 38.40743605819309 40.0 38.0 41.0 33.0 41.0 16-17 38.34305587780833 40.0 38.0 41.0 33.0 41.0 18-19 38.370815088341146 40.0 38.0 41.0 33.5 41.0 20-21 38.42375510324979 40.0 38.0 41.0 34.0 41.0 22-23 38.35873464088425 40.0 38.0 41.0 33.5 41.0 24-25 38.283989001169985 40.0 38.0 41.0 33.0 41.0 26-27 38.096033904831486 40.0 37.0 41.0 33.0 41.0 28-29 37.91313392605903 40.0 37.0 41.0 33.0 41.0 30-31 37.65277315653586 40.0 37.0 41.0 32.5 41.0 32-33 37.36933952716879 40.0 36.0 41.0 32.0 41.0 34-35 37.274992471626675 40.0 36.0 41.0 31.5 41.0 36-37 37.15298296366141 40.0 36.0 41.0 31.0 41.0 38-39 37.01510808769444 40.0 36.0 41.0 31.0 41.0 40-41 36.82231706052812 40.0 35.0 41.0 31.0 41.0 42-43 36.61019366431847 39.0 35.0 41.0 30.0 41.0 44-45 36.30849842274408 39.0 35.0 41.0 30.0 41.0 46-47 35.93908632699305 39.0 35.0 41.0 28.5 41.0 48-49 35.589948510863074 39.0 35.0 41.0 27.5 41.0 50-51 35.322391603765666 39.0 35.0 41.0 26.5 41.0 52-53 35.108418449204464 39.0 35.0 40.0 26.0 41.0 54-55 34.85889804361026 38.0 34.0 40.0 25.5 41.0 56-57 34.58857464443863 38.0 34.0 40.0 24.5 41.0 58-59 34.27007952924218 38.0 34.0 40.0 23.5 41.0 60-61 33.916293868201635 37.5 33.5 40.0 23.0 41.0 62-63 33.526124195945044 37.0 33.0 40.0 22.0 41.0 64-65 33.145347958946914 36.5 33.0 39.5 20.5 41.0 66-67 32.735219951917145 36.0 32.0 39.0 19.5 40.5 68-69 32.271750581289155 35.5 32.0 39.0 17.5 40.0 70-71 31.807924785379655 35.0 31.0 38.0 13.5 40.0 72-73 31.48101517028934 35.0 31.0 37.5 9.5 40.0 74-75 31.07586082629451 35.0 31.0 37.0 7.5 39.0 76-77 29.017298967748943 33.0 28.0 35.0 4.5 37.5 78-79 30.071447471700722 34.0 30.0 36.0 2.0 38.0 80-81 30.122992392640462 34.0 30.0 36.0 2.0 37.5 82-83 29.81902876579107 34.0 30.0 35.0 2.0 37.0 84-85 29.438349286902607 34.0 30.0 35.0 2.0 36.5 86-87 28.917425345688095 34.0 29.0 35.0 2.0 36.0 88-89 28.418189043625073 34.0 29.0 35.0 2.0 36.0 90-91 28.02358281457493 34.0 28.5 35.0 2.0 35.0 92-93 27.753317667734628 34.0 27.0 35.0 2.0 35.0 94-95 27.39289272191423 33.5 27.0 35.0 2.0 35.0 96-97 26.91926226878021 33.0 26.5 35.0 2.0 35.0 98-99 26.243867461136315 33.0 25.0 35.0 2.0 35.0 100-101 23.40389599490539 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0636542790348038 1101 2 0.05383514005145429 1101 3 0.05946192191582611 1101 4 0.07432224324738002 1101 5 0.14969704534707517 1101 6 0.23995027675588432 1101 7 0.11751553027125539 1101 8 -0.03237880591028386 1101 9 0.013973632263862612 1101 10-11 0.1050000564404101 1101 12-13 0.13789974022485296 1101 14-15 0.20090636964027198 1101 16-17 0.15919244577067815 1101 18-19 0.20948729743034278 1101 20-21 0.1526380627935282 1101 22-23 0.22749851611561667 1101 24-25 0.15511580856314566 1101 26-27 0.3239307540768479 1101 28-29 0.2590576903290511 1101 30-31 0.1571066588293064 1101 32-33 0.20025381971127132 1101 34-35 0.12472382330886944 1101 36-37 0.13806099482942358 1101 38-39 0.18251582641747888 1101 40-41 0.23248798936547388 1101 42-43 0.16928753220039994 1101 44-45 0.11104613384606665 1101 46-47 0.24517865615519696 1101 48-49 0.3231523596402255 1101 50-51 0.31086071360172696 1101 52-53 0.3527537478355427 1101 54-55 0.3975087956692249 1101 56-57 0.2469905483208379 1101 58-59 0.4959967118569466 1101 60-61 0.5504205729258942 1101 62-63 0.45590929644593103 1101 64-65 0.3826878470145232 1101 66-67 0.31295320667970117 1101 68-69 0.1418426406027251 1101 70-71 0.4130356752275368 1101 72-73 0.1658826086286176 1101 74-75 0.3665784707760693 1101 76-77 0.23283151755831 1101 78-79 0.25513223546466435 1101 80-81 0.36432239905575514 1101 82-83 0.3254047077848661 1101 84-85 0.23434335199691247 1101 86-87 0.2456965231738799 1101 88-89 0.5714953754505316 1101 90-91 0.563270547993536 1101 92-93 0.6250882749277267 1101 94-95 0.5621531699933264 1101 96-97 0.40468205443134053 1101 98-99 0.5942710506777971 1101 100-101 1.1064524589074338 1102 1 0.03394962557795367 1102 2 0.10418601644342829 1102 3 -0.03668551465476355 1102 4 0.03499075751300751 1102 5 -0.04090165889859776 1102 6 0.1267155936404123 1102 7 0.04980574636728363 1102 8 0.07445244012323826 1102 9 0.11256145180792743 1102 10-11 0.20931216064864344 1102 12-13 0.26901496556648397 1102 14-15 0.16784933910010835 1102 16-17 0.04024489231738215 1102 18-19 -0.01910056777103364 1102 20-21 0.08557318832829708 1102 22-23 0.011209587555605083 1102 24-25 -0.09898650032678802 1102 26-27 0.22028524652741055 1102 28-29 0.26140675869173435 1102 30-31 0.2223657868660851 1102 32-33 0.10391690532328823 1102 34-35 0.31591378965943306 1102 36-37 0.15547126680998957 1102 38-39 0.18424966258993436 1102 40-41 0.26685538457054747 1102 42-43 0.2832230549537229 1102 44-45 0.2665629060572101 1102 46-47 0.10148598460928326 1102 48-49 0.1177195968710194 1102 50-51 0.14816090581898322 1102 52-53 0.2675846103476971 1102 54-55 0.10476442635641092 1102 56-57 0.04927615302902666 1102 58-59 0.07156640088503252 1102 60-61 0.02330812354843914 1102 62-63 -0.15183509569553166 1102 64-65 -0.09943731792249366 1102 66-67 -0.1144368391145818 1102 68-69 -0.2539091754165952 1102 70-71 -0.1268813654485612 1102 72-73 0.09214035324904302 1102 74-75 -0.04694430993646037 1102 76-77 -0.11748214812977054 1102 78-79 -0.14775810025350467 1102 80-81 -0.30364512033718327 1102 82-83 -0.24082235512633687 1102 84-85 -0.08834193676271695 1102 86-87 0.041805835894461296 1102 88-89 0.32569356468179933 1102 90-91 0.34314298148225575 1102 92-93 0.2741764985389459 1102 94-95 0.37193456281508475 1102 96-97 0.2501172370377276 1102 98-99 0.3025642436301368 1102 100-101 0.5080358542586083 1103 1 -0.011945391284147178 1103 2 -0.009274230118435867 1103 3 -0.09696443017800505 1103 4 0.028380724732457452 1103 5 0.0746907838253108 1103 6 -0.044352144591506715 1103 7 -0.044938805747953836 1103 8 0.09357367872924982 1103 9 0.06695851661186225 1103 10-11 -0.05485997880462179 1103 12-13 0.016532833100704636 1103 14-15 0.07666705991946543 1103 16-17 -0.07818692121767867 1103 18-19 0.17359918800376306 1103 20-21 0.08517157603749581 1103 22-23 -0.247655652704033 1103 24-25 0.022945237941080165 1103 26-27 -0.1280693226408971 1103 28-29 0.021452682916759613 1103 30-31 0.007131930556788291 1103 32-33 0.07025898687697918 1103 34-35 -0.4118823867668837 1103 36-37 -0.2480131551266922 1103 38-39 0.04839967124004119 1103 40-41 -0.04621347255883279 1103 42-43 0.10213073692621322 1103 44-45 -0.09506003448620248 1103 46-47 0.06083832721658666 1103 48-49 0.2953760234540468 1103 50-51 0.2647104776946634 1103 52-53 0.1545636096759111 1103 54-55 0.002247131751609288 1103 56-57 0.06947697040833134 1103 58-59 -0.02369097923229191 1103 60-61 0.05490630455368972 1103 62-63 -0.18449207274016288 1103 64-65 0.05563277044344517 1103 66-67 -0.1917564730688497 1103 68-69 -0.1789497860020859 1103 70-71 -0.21081384066093634 1103 72-73 -0.1017816519589978 1103 74-75 -0.08080443876694332 1103 76-77 -0.26780422433612117 1103 78-79 -0.5259971174211628 1103 80-81 -0.3671852400007971 1103 82-83 -0.3530238420466958 1103 84-85 -0.17455601914322827 1103 86-87 -0.10499946471374244 1103 88-89 -0.0941873109340996 1103 90-91 0.0887724287064735 1103 92-93 -0.007374500043336951 1103 94-95 -0.0924505715761832 1103 96-97 -0.14753790887953144 1103 98-99 0.08207010885466914 1103 100-101 0.1041548752907353 1104 1 -0.12429641131831204 1104 2 -0.0012031866096293697 1104 3 -0.02179104291408862 1104 4 -0.005536420262707509 1104 5 -0.061744648917454015 1104 6 0.03527836982161858 1104 7 0.0760101281849046 1104 8 0.053192281290080246 1104 9 -0.005899040760283469 1104 10-11 0.0912920129399808 1104 12-13 0.05703913045632447 1104 14-15 0.1144053787774979 1104 16-17 0.21345492909004093 1104 18-19 0.17447348741885094 1104 20-21 -0.16801909868384968 1104 22-23 0.05044663214631839 1104 24-25 0.07873513991918202 1104 26-27 0.03691637659137115 1104 28-29 0.08527654021887798 1104 30-31 -0.1294311291748258 1104 32-33 -0.15783334961321316 1104 34-35 0.04624505259939582 1104 36-37 0.0729954471505252 1104 38-39 0.05882090575300225 1104 40-41 0.08279123327731952 1104 42-43 0.12733324932348467 1104 44-45 0.23474992129158778 1104 46-47 0.23661777905321912 1104 48-49 0.30186100164836915 1104 50-51 0.2809441943452953 1104 52-53 0.05916243550761635 1104 54-55 -0.14882013530731797 1104 56-57 0.01909321677860021 1104 58-59 0.024024606750117528 1104 60-61 0.17987003449061234 1104 62-63 0.09254085065964546 1104 64-65 0.3238190709406439 1104 66-67 0.11302059421566213 1104 68-69 0.27929437206339713 1104 70-71 0.20388715221158904 1104 72-73 0.12202972625976471 1104 74-75 0.1061489195681915 1104 76-77 0.29209264869356844 1104 78-79 0.23412904785687516 1104 80-81 0.19151881155614703 1104 82-83 0.07647315490116213 1104 84-85 0.25046094324717316 1104 86-87 0.5836926749909033 1104 88-89 0.7697388661248965 1104 90-91 0.6897330126926668 1104 92-93 0.4581711216964486 1104 94-95 0.5560450661987879 1104 96-97 0.5399259574110218 1104 98-99 0.3686314232599379 1104 100-101 0.8440112567492619 1105 1 0.04982069557265589 1105 2 0.05618752255324466 1105 3 -0.020479510355343677 1105 4 -0.09808980622739938 1105 5 0.001015478792233182 1105 6 -0.0035213121795649727 1105 7 0.01883198507692896 1105 8 0.01492826574724404 1105 9 0.06710889645620455 1105 10-11 0.003303483004309271 1105 12-13 -0.06193515330235755 1105 14-15 0.0658191556086436 1105 16-17 -0.0017193113051590103 1105 18-19 -0.20904881713728685 1105 20-21 0.045791530469159625 1105 22-23 -0.053086853384314736 1105 24-25 -0.0019474947095261541 1105 26-27 -0.0012179736579653877 1105 28-29 -0.040188448026221124 1105 30-31 -0.1923485557651432 1105 32-33 -0.1669333024235442 1105 34-35 -0.21268697302756578 1105 36-37 -0.14755671443369067 1105 38-39 -0.17604332830773473 1105 40-41 0.0023711177264118533 1105 42-43 0.0796727513237272 1105 44-45 -0.01498330830950323 1105 46-47 0.0400069322325578 1105 48-49 -0.07721654669978051 1105 50-51 0.05919316021144283 1105 52-53 0.09671010344542452 1105 54-55 0.17118211789285454 1105 56-57 0.15863220351872798 1105 58-59 0.23967605793858127 1105 60-61 0.16919390438318516 1105 62-63 -0.07760445949548256 1105 64-65 -0.155642796834762 1105 66-67 -0.0945607866984588 1105 68-69 -0.17864199416240467 1105 70-71 0.02605226502039315 1105 72-73 -0.10485902942106051 1105 74-75 0.005342656521946765 1105 76-77 -0.039183045228316615 1105 78-79 -0.11048516601008274 1105 80-81 -0.16086788010828812 1105 82-83 -0.034667006904118836 1105 84-85 0.12111131034874845 1105 86-87 0.08348436025710271 1105 88-89 0.11192557564487515 1105 90-91 0.18308926064672093 1105 92-93 0.2441309212041496 1105 94-95 0.02185487909814654 1105 96-97 -0.008375427244260436 1105 98-99 0.21161206699497725 1105 100-101 0.3711878268684252 1106 1 -0.05015560342078729 1106 2 -0.048996705580478306 1106 3 -0.0021783481670496485 1106 4 0.0071153962484160616 1106 5 0.026568519757219633 1106 6 -5.083479387195666E-4 1106 7 0.016895004585165907 1106 8 0.050957990584855395 1106 9 0.17520750942296104 1106 10-11 0.08189329408840962 1106 12-13 0.05359781521725182 1106 14-15 0.16749542502826387 1106 16-17 0.09706879120903267 1106 18-19 -0.034305805263713296 1106 20-21 0.010066982308920558 1106 22-23 -0.04294471026798874 1106 24-25 -0.027508925411346752 1106 26-27 0.0273585187189056 1106 28-29 0.1160939966591954 1106 30-31 0.11831396077837297 1106 32-33 0.1644057205496523 1106 34-35 0.23430719400789712 1106 36-37 0.24822125537584583 1106 38-39 0.21946302377177318 1106 40-41 0.13293429978580207 1106 42-43 0.1598948905807802 1106 44-45 0.08828386495743246 1106 46-47 0.3255495279751912 1106 48-49 0.20202955154823599 1106 50-51 0.2869692716942609 1106 52-53 0.18345106822321355 1106 54-55 0.2546998506503826 1106 56-57 0.10155525075504102 1106 58-59 0.01892739977211022 1106 60-61 0.133434748558507 1106 62-63 0.28305669371579256 1106 64-65 0.19410173567860767 1106 66-67 0.17464017476537919 1106 68-69 -0.009634416132612955 1106 70-71 0.09117707284298149 1106 72-73 0.19137785899333792 1106 74-75 0.40519017305497584 1106 76-77 0.2330256368908472 1106 78-79 0.20929437268024031 1106 80-81 0.029692931850441084 1106 82-83 0.18905528861177956 1106 84-85 0.18210994072421016 1106 86-87 0.18754383929639218 1106 88-89 0.5023446010510995 1106 90-91 0.569505332865571 1106 92-93 0.7618385095113673 1106 94-95 1.020624209576173 1106 96-97 0.8023631212893179 1106 98-99 0.6302913965711063 1106 100-101 1.0635094348453862 1107 1 -0.1818967348304419 1107 2 -0.14397081601974548 1107 3 -0.2513540698586887 1107 4 -0.02302102921531457 1107 5 -0.03679777778101112 1107 6 -0.07197558930548809 1107 7 -0.017804176695790375 1107 8 -0.10481580230074172 1107 9 -0.19119082300461088 1107 10-11 -0.20148698994339753 1107 12-13 -0.2075178622288476 1107 14-15 -0.29168448429852134 1107 16-17 -0.2552448512334209 1107 18-19 -0.11918906584164546 1107 20-21 -0.1301533309428109 1107 22-23 -0.21669571950884148 1107 24-25 -0.27279627719452293 1107 26-27 -0.3109255068557957 1107 28-29 -0.26205932897774886 1107 30-31 -0.20074175347320278 1107 32-33 -0.14355016276600452 1107 34-35 -0.07301950491983433 1107 36-37 -0.14761781474054914 1107 38-39 0.11974639054729863 1107 40-41 0.0420236502035749 1107 42-43 -0.044165037283832476 1107 44-45 -0.07095745609078108 1107 46-47 -0.2554323737515034 1107 48-49 -0.3826209471752122 1107 50-51 -0.3066389215230174 1107 52-53 -0.042094649218441305 1107 54-55 -0.1354557776663512 1107 56-57 -0.32100259420270305 1107 58-59 -0.014345595592395455 1107 60-61 -0.23814565247769792 1107 62-63 -0.26360946032059474 1107 64-65 -0.30638560945423166 1107 66-67 -0.3363858789755838 1107 68-69 -0.02856554044510773 1107 70-71 -0.24163549047811372 1107 72-73 -0.11917373464602576 1107 74-75 -0.09383201933308882 1107 76-77 -0.17907507001937617 1107 78-79 -0.1673361293060296 1107 80-81 -0.1963650694941741 1107 82-83 -0.14978050725191494 1107 84-85 -0.33617069733422156 1107 86-87 -0.10850585951600422 1107 88-89 -0.08034664554450188 1107 90-91 0.1320642377210497 1107 92-93 0.36844863444421705 1107 94-95 0.278244822300973 1107 96-97 0.42538191784818125 1107 98-99 0.2688823768414217 1107 100-101 0.4382367969365859 1108 1 -0.12099767991966104 1108 2 -0.19811435590158766 1108 3 -0.06633209209052637 1108 4 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-0.8188200482516237 2316 48-49 -0.6550321904110561 2316 50-51 -0.6317400097711356 2316 52-53 -0.511425799786501 2316 54-55 -0.46091975776499794 2316 56-57 -0.4275393492913082 2316 58-59 -0.4316507068535884 2316 60-61 -0.5542975455848946 2316 62-63 -0.5748565430804575 2316 64-65 -0.576923834917352 2316 66-67 -0.6123234564480811 2316 68-69 -0.5945212411384091 2316 70-71 -0.4804935565936752 2316 72-73 -0.6827687148558077 2316 74-75 -0.5511522617739146 2316 76-77 -0.5747566997414602 2316 78-79 -0.4287439274201894 2316 80-81 -0.6999012347232387 2316 82-83 -0.9558919650598625 2316 84-85 -0.9723316520518175 2316 86-87 -1.164778887547154 2316 88-89 -1.2112561405734361 2316 90-91 -1.7184085047838913 2316 92-93 -1.9635132042078247 2316 94-95 -1.6602866838925543 2316 96-97 -1.7775323302518728 2316 98-99 -2.413916378469448 2316 100-101 -3.180644692586995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 10.0 5 40.0 6 145.0 7 300.0 8 596.0 9 1019.0 10 1723.0 11 2675.0 12 3778.0 13 5052.0 14 5626.0 15 7272.0 16 8836.0 17 11715.0 18 11425.0 19 7576.0 20 6897.0 21 6891.0 22 7489.0 23 7987.0 24 9406.0 25 10750.0 26 12456.0 27 14643.0 28 17977.0 29 21704.0 30 26034.0 31 31511.0 32 38347.0 33 48095.0 34 62802.0 35 86958.0 36 126292.0 37 201597.0 38 187090.0 39 20116.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.202779900734825 21.039479070947735 15.287372387152029 22.470368641165408 2 15.902491521323808 22.480167055838315 38.92855203463546 22.68878938820242 3 18.178577951986256 26.292930240364914 31.97470466561681 23.553787142032018 4 13.08604066802589 19.019089980105345 37.08925935616364 30.805609995705126 5 12.81116865037247 37.89383265783667 34.90746271603963 14.387535975751234 6 29.249087956083663 37.63337562386766 18.0753034798363 15.042232940212374 7 25.32683013521452 34.78572521684183 22.3161719332369 17.57127271470674 8 24.660976368312706 35.320560604639454 21.328350619794932 18.690112407252908 9 25.438793090681106 17.07030266529099 21.950663237348632 35.540241006679274 10-11 23.509011833121882 27.58958764260714 28.420720058054865 20.480680466216118 12-13 24.627703426520608 25.71396130662941 28.65851792246516 20.999817344384823 14-15 21.908146571343096 27.207105627691096 27.453642315450455 23.431105485515353 16-17 20.418616063075778 29.940161737575576 28.449462808305775 21.191759391042872 18-19 20.256764555314813 28.0210636584753 31.37822615639574 20.34394562981415 20-21 21.17457433836706 27.361366856398128 31.265655314044245 20.198403491190568 22-23 20.912488213776182 27.666648565659756 30.970148148513825 20.450715072050237 24-25 20.67488781489581 27.75496502391801 31.29325112185104 20.27689603933513 26-27 20.467954043824534 27.812145024680735 31.31518525484667 20.40471567664806 28-29 20.315549916817645 27.890228911915564 31.188742490139067 20.605478681127725 30-31 20.600245844584755 27.615801191704474 31.135031865999892 20.648921097710883 32-33 20.35272280282573 27.826151347455408 31.044049623087673 20.777076226631188 34-35 20.714389744779695 27.9539670353813 30.89698618369246 20.434657036146543 36-37 20.82336374489835 27.739386153142753 30.67994979573659 20.757300306222305 38-39 20.64083505352635 27.734810666858888 30.801979411005252 20.822374868609508 40-41 20.79602392870561 27.5890056711447 30.871130679229058 20.743839720920633 42-43 20.65353152877405 27.721772700569215 30.65817210618036 20.96652366447638 44-45 20.655046884271826 27.8799245270351 30.438738444313017 21.02629014438006 46-47 20.981467585553208 27.758190004146837 29.904918939199465 21.35542347110049 48-49 20.790207684371097 27.904989460277342 29.75459971268765 21.55020314266391 50-51 20.71138155192569 28.238740482133267 29.548385122113046 21.501492843828 52-53 20.727117447560563 28.244482072598203 29.5742149511026 21.45418552873864 54-55 20.503438368539793 28.245173201952934 29.363124299614444 21.888264129892825 56-57 20.69488119980056 28.494325334333826 28.903868843395024 21.906924622470587 58-59 20.547259481738518 28.2301988934948 29.249423767202444 21.973117857564237 60-61 20.54874048271746 28.256511344220662 29.283388056319502 21.91136011674237 62-63 20.394141197727173 28.263784328148216 29.313609817986148 22.028464656138464 64-65 20.267615159428733 28.283580247523044 29.108739330690586 22.34006526235764 66-67 20.208160651880608 28.327393667307284 28.886128728253247 22.57831695255886 68-69 20.305225332498047 28.007391204111663 28.965502942017157 22.721880521373127 70-71 20.173210138860366 27.844100014217787 28.816241054643687 23.166448792278164 72-73 20.07933177664674 27.824226058538652 28.732221931509084 23.364220233305524 74-75 19.895590101052985 27.920687969906254 28.59680994436409 23.586911984676675 76-77 19.7669948202403 27.695604863696516 28.896852512741855 23.640547803321333 78-79 19.63893208519733 27.662367227936098 28.800939064974546 23.897761621892027 80-81 19.619691074899208 27.513428896823715 28.741862564910654 24.125017463366426 82-83 19.45761155568281 27.455804746084013 28.782032611432268 24.30455108680091 84-85 19.4086401042618 27.170565788109613 28.77196186940617 24.648832238222415 86-87 19.339236211880674 27.150653926152547 28.678961885743753 24.831147976223026 88-89 19.369344829806305 26.843356428631566 28.664401709613358 25.12289703194877 90-91 19.163866039167697 26.716228986630746 28.754363063299877 25.36554191090168 92-93 18.98764359446504 26.564790908687 28.74757487646063 25.699990620387332 94-95 18.841380304482126 26.299163387503093 28.95097866433262 25.90847764368216 96-97 18.86848246184347 26.052232620433053 28.858515384300198 26.220769533423276 98-99 18.689763608494 26.0529751957999 28.872740556804803 26.3845206389013 100-101 18.59430020837725 25.671869762491404 29.199917261248594 26.53391276788275 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2266.0 1 1506.0 2 830.0 3 887.0 4 784.5 5 789.5 6 1171.5 7 1928.5 8 2626.0 9 2828.5 10 2371.0 11 1871.5 12 1829.0 13 1888.0 14 1950.0 15 2042.0 16 2158.0 17 2270.0 18 2413.5 19 2584.5 20 2857.0 21 3237.0 22 3661.5 23 4280.0 24 4971.0 25 5952.0 26 7453.5 27 9059.5 28 10525.0 29 12163.5 30 13940.5 31 16125.0 32 18684.0 33 20750.0 34 22843.5 35 24969.0 36 27537.0 37 29819.5 38 30609.5 39 30885.5 40 31223.5 41 31684.5 42 32457.5 43 33789.5 44 35338.5 45 36372.0 46 36974.5 47 37260.0 48 37515.5 49 37465.0 50 36654.5 51 35184.0 52 33126.5 53 31347.0 54 29506.5 55 26831.0 56 23793.5 57 20783.0 58 18416.0 59 15970.0 60 13145.5 61 10654.5 62 8335.0 63 6253.5 64 4429.0 65 3140.5 66 2251.5 67 1688.5 68 1272.0 69 937.5 70 713.5 71 516.5 72 370.5 73 309.0 74 271.0 75 211.5 76 145.0 77 91.0 78 63.5 79 43.0 80 26.0 81 22.5 82 20.0 83 11.5 84 7.5 85 7.5 86 7.0 87 5.5 88 4.0 89 1.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39710318067602324 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.949310598468655E-4 16-17 0.0021227544466769018 18-19 4.9366382480858185E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.9366382480858185E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0081454531093416 36-37 0.016982035573415214 38-39 0.010811237763307942 40-41 0.007553056519571303 42-43 0.002961982948851491 44-45 0.00217212082915776 46-47 0.0014809914744257455 48-49 0.0 50-51 2.961982948851491E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.417629722511564E-4 60-61 6.417629722511564E-4 62-63 0.0 64-65 0.0 66-67 0.0011354267970597382 68-69 9.379612671363055E-4 70-71 0.001875922534272611 72-73 0.0 74-75 0.0 76-77 0.09991755814125697 78-79 0.03342104093954099 80-81 4.9366382480858185E-5 82-83 0.0 84-85 0.0 86-87 0.15012316912428975 88-89 0.3208814861255782 90-91 0.2758099789205547 92-93 0.0 94-95 4.9366382480858185E-5 96-97 4.9366382480858185E-5 98-99 2.468319124042909E-4 100-101 8.392285021745892E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1012835.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.5516473305786 #Duplication Level Percentage of deduplicated Percentage of total 1 84.40862169735303 58.707586879545225 2 10.116455939766128 14.072323515159017 3 2.461696420259969 5.136451237706076 4 0.9895339058341757 2.7529485296091427 5 0.5207046402635432 1.8107932751502882 6 0.3283165867416425 1.3700975672300422 7 0.22800986396200967 1.1100923152325233 8 0.15278647603747225 0.8501240878592135 9 0.12183695383438883 0.762656476042926 >10 0.6231260735726917 7.616735807204426 >50 0.03398957540602605 1.5811411596863993 >100 0.012932286190322545 1.445167827721273 >500 8.526774765470245E-4 0.41572891318003974 >1k 8.526774765470245E-4 1.2414539109703517 >5k 2.8422582551567484E-4 1.1266984977031083 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6113 0.6035533922109722 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5286 0.5219013955876327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3553 0.3507975139089783 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3002 0.29639576041507254 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 2124 0.20970839277868555 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1476 0.14572956108349336 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.12716780127069066 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1117 0.11028449846223719 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 9.873276496171637E-5 0.0 0.0 0.0 3 0.0 9.873276496171637E-5 0.0 0.0 0.0 4 0.0 9.873276496171637E-5 0.0 0.0 0.0 5 0.0 1.9746552992343274E-4 0.0 0.0 0.0 6 0.0 1.9746552992343274E-4 0.0 0.0 0.0 7 0.0 1.9746552992343274E-4 0.0 0.0 0.0 8 0.0 1.9746552992343274E-4 0.0 0.0 0.0 9 0.0 1.9746552992343274E-4 0.0 0.0 0.0 10-11 0.0 1.9746552992343274E-4 0.0 0.0 0.0 12-13 0.0 1.9746552992343274E-4 0.0 0.0 0.0 14-15 0.0 2.4683191240429094E-4 0.0 4.9366382480858185E-5 0.0 16-17 0.0 2.9619829488514913E-4 0.0 1.9746552992343274E-4 0.0 18-19 0.0 2.9619829488514913E-4 0.0 4.936638248085819E-4 0.0 20-21 0.0 2.9619829488514913E-4 0.0 9.379612671363055E-4 0.0 22-23 0.0 2.9619829488514913E-4 0.0 0.002418952741562051 0.0 24-25 0.0 2.9619829488514913E-4 0.0 0.005232836542970968 0.0 26-27 0.0 2.9619829488514913E-4 0.0 0.009922642878652495 0.0 28-29 0.0 2.9619829488514913E-4 0.0 0.023992061885697077 0.0 30-31 0.0 2.9619829488514913E-4 0.0 0.06565728869954138 0.0 32-33 0.0 2.9619829488514913E-4 0.0 0.15412184610523927 0.0 34-35 0.0 2.9619829488514913E-4 0.0 0.29377934214358703 0.0 36-37 0.0 2.9619829488514913E-4 0.0 0.5105471276170354 0.0 38-39 0.0 2.9619829488514913E-4 0.0 0.7897140205462885 0.0 40-41 0.0 3.455646773660073E-4 0.0 1.0896641604999826 0.0 42-43 0.0 3.949310598468655E-4 0.0 1.3863561192099403 0.0 44-45 0.0 3.949310598468655E-4 0.0 1.760306466502441 0.0 46-47 9.873276496171637E-5 3.949310598468655E-4 0.0 2.1785878252627526 0.0 48-49 9.873276496171637E-5 3.949310598468655E-4 0.0 2.600423563561686 0.0 50-51 9.873276496171637E-5 3.949310598468655E-4 0.0 3.034699630245795 0.0 52-53 9.873276496171637E-5 3.949310598468655E-4 0.0 3.460583411908159 0.0 54-55 9.873276496171637E-5 3.949310598468655E-4 0.0 3.935290545844091 0.0 56-57 9.873276496171637E-5 3.949310598468655E-4 0.0 4.488638327072031 0.0 58-59 9.873276496171637E-5 3.949310598468655E-4 0.0 5.084342464468547 0.0 60-61 9.873276496171637E-5 3.949310598468655E-4 0.0 5.707741142436824 0.0 62-63 9.873276496171637E-5 3.949310598468655E-4 0.0 6.3197361860520225 0.0 64-65 9.873276496171637E-5 3.949310598468655E-4 0.0 6.971323068416869 0.0 66-67 9.873276496171637E-5 3.949310598468655E-4 0.0 7.699872141069374 0.0 68-69 9.873276496171637E-5 3.949310598468655E-4 0.0 8.501977123618358 0.0 70-71 9.873276496171637E-5 3.949310598468655E-4 0.0 9.350930803141676 0.0 72-73 9.873276496171637E-5 3.949310598468655E-4 0.0 10.201661672434305 0.0 74-75 9.873276496171637E-5 3.949310598468655E-4 0.0 11.072336560249202 0.0 76-77 9.873276496171637E-5 3.949310598468655E-4 0.0 11.996425873908386 0.0 78-79 9.873276496171637E-5 3.949310598468655E-4 0.0 13.004043106725181 0.0 80-81 9.873276496171637E-5 3.949310598468655E-4 0.0 14.050018018729606 0.0 82-83 9.873276496171637E-5 3.949310598468655E-4 0.0 15.119195130500032 0.0 84-85 9.873276496171637E-5 3.949310598468655E-4 0.0 16.174500288793336 0.0 86-87 9.873276496171637E-5 3.949310598468655E-4 0.0 17.269792216896136 0.0 88-89 9.873276496171637E-5 3.949310598468655E-4 0.0 18.431876860495542 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3330 0.0 47.90552 1 GTATCAA 5310 0.0 34.16768 1 TATCAAC 6720 0.0 26.762705 2 ATCAACG 6840 0.0 26.154434 3 TCAACGC 6885 0.0 26.052412 4 CAACGCA 6945 0.0 25.75901 5 AACGCAG 7085 0.0 25.183035 6 GTGGACG 100 0.001663145 23.726303 4 CGGTATC 105 0.0022088194 22.59648 2 ACGCAGA 7995 0.0 22.197966 7 CGCAGAG 8115 0.0 21.81124 8 GAGTACT 5145 0.0 21.584076 12-13 GTACATG 3950 0.0 20.856144 1 GGACCGT 115 0.003744571 20.631567 6 GCAGAGT 8495 0.0 20.276983 9 AGTACTT 5270 0.0 20.126575 12-13 TACATGG 4005 0.0 19.786728 2 AGAGTAC 7435 0.0 19.53185 10-11 GTACTTT 5670 0.0 19.460001 14-15 ACATGGG 3840 0.0 19.401196 3 >>END_MODULE