FastQCFastQC Report
Thu 26 May 2016
SRR938784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012228
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65610.6481741267777615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51590.5096677823573345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34390.33974559091429996No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27540.27207309025239373No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.14749641385142478No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.12181050119143116No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10590.10462069810358932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31950.054.1399961
GTATCAA52800.036.090941
TATCAAC64950.029.0008132
ATCAACG66650.028.332293
TCAACGC67600.027.9341324
CAACGCA68750.027.3288445
AACGCAG70300.026.793786
ACGCAGA78550.023.7984587
CGCAGAG79050.023.587918
GTAGTAC1652.4535639E-623.004083
GAGTACT52500.022.65081812-13
GCAGAGT82100.021.7259589
CAGAGTA79700.020.72743810-11
GTACATG38600.020.6828171
GTACTTT57550.020.37450814-15
TACATGG39250.020.3080582
ACATGGG36650.019.8068893
AGTACTT53950.019.57825912-13
AGAGTAC74350.018.93077710-11
ATACTGC2051.6047527E-518.5154786
Produced by FastQC (version 0.11.5)