FastQCFastQC Report
Thu 26 May 2016
SRR938784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012228
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59960.5923566627281601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52690.5205348992519472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35710.35278613118783514No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29520.29163390066269657No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19730.19491656030064372No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14460.1428531911782721No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.11785882232066293No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.11647573471589405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34100.046.1617051
GTATCAA54850.034.7677041
TATCAAC68100.027.8766382
ATCAACG68650.027.44593
TCAACGC69000.027.3066834
CAACGCA69800.026.9937115
AACGCAG70950.026.4827446
TATTCCG1501.0677195E-625.3119855
CGCAGAG80250.023.2351938
ACGCAGA80450.023.1184527
GTACATG38000.022.7769741
GTGGTAT18650.022.184451
TACATGG38550.021.790962
TACACCG1100.002891237821.5727165
GAGTACT50900.021.48794412-13
GCAGAGT84600.021.4796499
ACATGGG38150.021.1486133
TGGTATC19000.020.9823042
AGACCCG1150.003741200520.6347715
AGTACTT52800.019.99575412-13
Produced by FastQC (version 0.11.5)