##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938784_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012228 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.989193146208166 31.0 31.0 34.0 30.0 34.0 2 32.185854372730255 33.0 31.0 34.0 30.0 34.0 3 32.255819835056926 34.0 31.0 34.0 30.0 34.0 4 35.904167835704996 37.0 35.0 37.0 35.0 37.0 5 35.80748408461335 37.0 35.0 37.0 35.0 37.0 6 35.82610538337212 37.0 35.0 37.0 35.0 37.0 7 35.74294230153681 37.0 35.0 37.0 33.0 37.0 8 35.73272424789672 37.0 35.0 37.0 33.0 37.0 9 37.35757161430033 39.0 37.0 39.0 34.0 39.0 10-11 37.299428093275424 39.0 37.0 39.0 33.5 39.0 12-13 37.223433850871544 39.0 37.0 39.0 33.0 39.0 14-15 38.41673516243376 40.0 38.0 41.0 33.0 41.0 16-17 38.35180759670746 40.0 38.0 41.0 33.0 41.0 18-19 38.47484558814813 40.0 38.0 41.0 33.5 41.0 20-21 38.475633454122985 40.0 38.0 41.0 34.0 41.0 22-23 38.38376778749452 40.0 38.0 41.0 34.0 41.0 24-25 38.297479421632275 40.0 38.0 41.0 33.0 41.0 26-27 38.096498516144585 40.0 37.0 41.0 33.0 41.0 28-29 37.90610070063266 40.0 37.0 41.0 33.0 41.0 30-31 37.63995611660614 40.0 37.0 41.0 32.5 41.0 32-33 37.4238931347483 40.0 36.0 41.0 32.0 41.0 34-35 37.346239187218686 40.0 36.0 41.0 32.0 41.0 36-37 37.19118864524593 40.0 36.0 41.0 31.0 41.0 38-39 37.025633552914954 40.0 36.0 41.0 31.0 41.0 40-41 36.81613924926005 40.0 35.0 41.0 31.0 41.0 42-43 36.58547481397471 39.0 35.0 41.0 30.0 41.0 44-45 36.28694523368253 39.0 35.0 41.0 30.0 41.0 46-47 35.89323403422944 39.0 35.0 41.0 28.5 41.0 48-49 35.52225437352059 39.0 35.0 41.0 27.0 41.0 50-51 35.254895142201164 39.0 35.0 40.5 26.0 41.0 52-53 35.02976700901378 39.0 35.0 40.0 26.0 41.0 54-55 34.75175750917778 38.0 34.0 40.0 25.0 41.0 56-57 34.45745079171886 38.0 34.0 40.0 24.0 41.0 58-59 34.13131181907633 38.0 34.0 40.0 23.0 41.0 60-61 33.75652175201634 37.5 33.0 40.0 22.0 41.0 62-63 33.37718626633525 37.0 33.0 40.0 21.5 41.0 64-65 32.966460125584355 36.0 32.5 39.0 19.5 41.0 66-67 32.54104361863138 36.0 32.0 39.0 18.0 40.0 68-69 32.21041010523321 35.5 32.0 39.0 15.5 40.0 70-71 31.80027968007208 35.0 31.0 38.0 12.5 40.0 72-73 31.3312776370541 35.0 31.0 37.5 8.0 39.5 74-75 30.85160606108505 35.0 30.5 37.0 7.0 39.0 76-77 28.89139106999609 32.5 28.0 35.0 2.0 37.5 78-79 29.835358733407887 34.0 29.5 36.0 2.0 38.0 80-81 29.887396910577458 34.0 30.0 35.5 2.0 37.0 82-83 29.570847674634567 34.0 30.0 35.0 2.0 37.0 84-85 29.18746566978981 34.0 29.5 35.0 2.0 36.5 86-87 28.61717123019715 34.0 29.0 35.0 2.0 36.0 88-89 28.03203477872574 34.0 28.0 35.0 2.0 35.5 90-91 27.5272245976203 33.0 26.5 35.0 2.0 35.0 92-93 27.24086520033036 33.0 26.0 35.0 2.0 35.0 94-95 26.918857213987362 33.0 26.0 35.0 2.0 35.0 96-97 26.352490743192245 33.0 25.0 35.0 2.0 35.0 98-99 25.57974191585295 33.0 22.0 34.5 2.0 35.0 100-101 22.2539813164623 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21849389572603428 1101 2 0.21514722470663372 1101 3 0.10212931989843099 1101 4 0.048648747023356975 1101 5 -0.007888191238556885 1101 6 0.037477978927377364 1101 7 0.18154520265684226 1101 8 0.1536667204736233 1101 9 0.3210946972888564 1101 10-11 0.2699122453290457 1101 12-13 0.2057211832121908 1101 14-15 0.3821623393845286 1101 16-17 0.41978200661719 1101 18-19 0.5041779944959117 1101 20-21 0.3409164097474786 1101 22-23 0.0975361159091932 1101 24-25 0.3601345519219237 1101 26-27 0.2439034345141593 1101 28-29 0.4291321179628511 1101 30-31 0.3055310382324663 1101 32-33 0.4444126142113163 1101 34-35 0.4710851512645249 1101 36-37 0.5075206775672214 1101 38-39 0.48119315463245016 1101 40-41 0.4363425224397517 1101 42-43 0.4405366445213943 1101 44-45 0.3184654134469369 1101 46-47 0.3322502232099751 1101 48-49 0.10555026830452618 1101 50-51 0.18294699484211918 1101 52-53 0.12608721606962803 1101 54-55 0.39044821830555776 1101 56-57 0.32771592999543486 1101 58-59 0.35858709841246394 1101 60-61 0.5337146959529733 1101 62-63 0.47736701601379394 1101 64-65 0.47291576248168354 1101 66-67 0.19934276646146998 1101 68-69 0.2191155797474309 1101 70-71 0.39198222479028644 1101 72-73 0.21746018611672469 1101 74-75 0.09504803021311758 1101 76-77 0.22673478872546582 1101 78-79 0.12540872450125917 1101 80-81 0.059708856147487666 1101 82-83 0.12836124052585163 1101 84-85 0.3558558769902298 1101 86-87 0.3038264153557151 1101 88-89 0.43296320740398286 1101 90-91 0.34222431700801437 1101 92-93 0.24850361006476973 1101 94-95 0.1354232964734372 1101 96-97 -0.09677603032030291 1101 98-99 -0.28259180363669145 1101 100-101 0.3849081195379753 1102 1 0.2524561598769779 1102 2 0.22707725615317287 1102 3 0.255005368116457 1102 4 0.1540929294132951 1102 5 0.11406542511364393 1102 6 0.03823793699866229 1102 7 0.11416443745768134 1102 8 0.15632002236041842 1102 9 0.27498620672281504 1102 10-11 0.22868779564351627 1102 12-13 0.24867846832749052 1102 14-15 0.3909411653803403 1102 16-17 0.2798481753802875 1102 18-19 0.1468830521479063 1102 20-21 0.19755621582714156 1102 22-23 0.1906473580475634 1102 24-25 0.20685559489886174 1102 26-27 0.2493607196294647 1102 28-29 0.35230428777417444 1102 30-31 0.40303496905007563 1102 32-33 0.4835504548821703 1102 34-35 0.5719859636335016 1102 36-37 0.5466257614246643 1102 38-39 0.4615914774836014 1102 40-41 0.5985084029429046 1102 42-43 0.597517121460605 1102 44-45 0.829527389337926 1102 46-47 0.7101164443839778 1102 48-49 0.3825779150550517 1102 50-51 0.4424399193704289 1102 52-53 0.18215705359583012 1102 54-55 0.07675259461582584 1102 56-57 0.3475174237061296 1102 58-59 0.16879739715460573 1102 60-61 0.13843822844773257 1102 62-63 0.0941286774393788 1102 64-65 0.24331998155925305 1102 66-67 0.4007120012623062 1102 68-69 0.3573984675880979 1102 70-71 0.2359516954401819 1102 72-73 0.0550093213368541 1102 74-75 0.08579750610410031 1102 76-77 0.010123808641608889 1102 78-79 -0.015733833150729737 1102 80-81 -0.09795951387347657 1102 82-83 0.05551991453004135 1102 84-85 0.09278263275542642 1102 86-87 0.2686816302404118 1102 88-89 0.3494169547834396 1102 90-91 0.47604900254260585 1102 92-93 0.4466528501066982 1102 94-95 0.630594942175744 1102 96-97 0.6637290797425912 1102 98-99 0.9553111051683416 1102 100-101 1.555626804024346 1103 1 0.13696416123891453 1103 2 0.13178337634384008 1103 3 0.05787074945796178 1103 4 0.019990316202530778 1103 5 -0.06357651393981456 1103 6 0.011982404142003134 1103 7 0.12057510310289388 1103 8 0.012992434685919818 1103 9 0.12929970689305748 1103 10-11 0.17195739472420968 1103 12-13 0.18835224258693728 1103 14-15 0.13125866623154536 1103 16-17 0.06824779659240932 1103 18-19 -0.12913467427361525 1103 20-21 0.11685364943286203 1103 22-23 0.11441895746873598 1103 24-25 0.2684261813816633 1103 26-27 0.33642553917321294 1103 28-29 0.1616197031640354 1103 30-31 0.18697372458293415 1103 32-33 0.44123048552568633 1103 34-35 0.381302975039695 1103 36-37 0.5784301111012056 1103 38-39 0.5171243341400569 1103 40-41 0.6808915745465853 1103 42-43 0.6307871461456571 1103 44-45 0.8979661661510008 1103 46-47 1.0071377458821118 1103 48-49 0.9146519914935922 1103 50-51 1.049618800882179 1103 52-53 0.9745169233609019 1103 54-55 0.865134127642726 1103 56-57 0.6558119206417743 1103 58-59 0.6064671068924312 1103 60-61 0.7827553462550085 1103 62-63 0.6479117681782256 1103 64-65 0.7158849926249644 1103 66-67 0.5935990884260747 1103 68-69 0.43366942860274094 1103 70-71 0.3395081518583005 1103 72-73 0.6506708169105693 1103 74-75 0.777885521085377 1103 76-77 0.760499936493158 1103 78-79 0.5733281348438233 1103 80-81 0.4964570889525355 1103 82-83 0.5175863300901362 1103 84-85 0.3295096288398689 1103 86-87 0.29370131444197867 1103 88-89 0.31666935944805985 1103 90-91 0.21603985204890463 1103 92-93 0.2754157246385347 1103 94-95 0.40136124282266294 1103 96-97 0.531947606300303 1103 98-99 0.5848187873270021 1103 100-101 1.0165472798500161 1104 1 0.12233567794926614 1104 2 0.10186390273951673 1104 3 0.09241751671083875 1104 4 -0.0220491388597992 1104 5 -0.04751841022772396 1104 6 0.09883030647657165 1104 7 0.05222919649382618 1104 8 -0.0011222467158873428 1104 9 0.010001266963776345 1104 10-11 0.10823724343467944 1104 12-13 -0.02101279673274803 1104 14-15 0.1070556232116644 1104 16-17 0.13026860710719745 1104 18-19 0.14120408628444636 1104 20-21 0.048867711811077186 1104 22-23 -0.022737431109071338 1104 24-25 0.20246929971813898 1104 26-27 0.11556302886641134 1104 28-29 0.06790418737257653 1104 30-31 -0.0270729626768329 1104 32-33 0.16178840668940353 1104 34-35 0.27435167648490477 1104 36-37 0.4510810827098055 1104 38-39 0.3288605537888216 1104 40-41 0.3648111137862742 1104 42-43 0.43693102708309084 1104 44-45 0.4844658933539918 1104 46-47 0.39827532992615744 1104 48-49 0.40286531465344666 1104 50-51 0.4457908229848826 1104 52-53 0.510413830704266 1104 54-55 0.3572809580696443 1104 56-57 0.2770794216980974 1104 58-59 0.44755618229516614 1104 60-61 0.5570501762388957 1104 62-63 0.5146545308530293 1104 64-65 0.4338759554548375 1104 66-67 0.3782596920253525 1104 68-69 0.3885543410820773 1104 70-71 0.375390701464287 1104 72-73 0.6505226245497013 1104 74-75 0.33383967477880105 1104 76-77 0.3912671319347787 1104 78-79 0.30364367688942906 1104 80-81 0.33728772508234783 1104 82-83 0.42957237436939977 1104 84-85 0.5020886066510108 1104 86-87 0.5938134073488435 1104 88-89 0.5510045551850418 1104 90-91 0.42033866117714425 1104 92-93 0.6075024987010771 1104 94-95 0.790597452215902 1104 96-97 0.9946841500237191 1104 98-99 1.0680181834815912 1104 100-101 1.909736994787398 1105 1 0.06233024867751169 1105 2 0.03614576178603812 1105 3 0.030156544392141882 1105 4 0.006861733256897651 1105 5 -0.0542890314801312 1105 6 -0.06657485292844711 1105 7 -0.04934409468280876 1105 8 -0.0026532215096963796 1105 9 -0.13512396557671735 1105 10-11 0.007499301607730047 1105 12-13 0.07706440272112758 1105 14-15 0.1500945782365335 1105 16-17 -0.03987618678008431 1105 18-19 -0.0654316015963019 1105 20-21 -0.056124822940255115 1105 22-23 -0.13852862770459495 1105 24-25 -0.0756980416153894 1105 26-27 -0.014640688587896022 1105 28-29 -0.10222064595676983 1105 30-31 -0.12945964658941733 1105 32-33 0.030357114921933714 1105 34-35 -0.02991409435192338 1105 36-37 0.13560646056316727 1105 38-39 0.13452056959759062 1105 40-41 0.09908618624310606 1105 42-43 0.17056736168591868 1105 44-45 0.0554127024592006 1105 46-47 -0.0799297368618781 1105 48-49 0.0403117205553869 1105 50-51 0.034816907441999945 1105 52-53 0.2476919691027888 1105 54-55 0.09228958103067697 1105 56-57 0.29714776664847165 1105 58-59 0.315627872635055 1105 60-61 0.27920819199975 1105 62-63 0.09329513701525372 1105 64-65 0.03778962204964387 1105 66-67 0.014841805768597283 1105 68-69 0.005577104102350461 1105 70-71 0.25363828424071144 1105 72-73 0.1996425635502419 1105 74-75 0.1880258029894577 1105 76-77 0.2697732039365057 1105 78-79 -0.008759015390623404 1105 80-81 0.23982730686873666 1105 82-83 0.209004217048264 1105 84-85 0.22089624276205555 1105 86-87 0.3329139446870677 1105 88-89 0.1454169962015932 1105 90-91 0.16885259763869342 1105 92-93 0.20834623148509834 1105 94-95 0.2701099355488381 1105 96-97 0.1834451582714216 1105 98-99 0.3463202585811942 1105 100-101 0.8678896850713969 1106 1 -0.032856237898617024 1106 2 -0.020961057553073204 1106 3 -0.17584493446777572 1106 4 -0.010769476540254175 1106 5 -0.07005605836689455 1106 6 -0.06173344016849569 1106 7 -0.012363817367315733 1106 8 0.044665418173302385 1106 9 -0.06182480080089903 1106 10-11 -0.07624504292370915 1106 12-13 -0.021865569945326513 1106 14-15 0.0038991307058182656 1106 16-17 -0.005041524488866855 1106 18-19 -0.13373571216351365 1106 20-21 -0.13163447357423053 1106 22-23 -0.05037989497828477 1106 24-25 -0.07797583706057054 1106 26-27 -0.05098786558479418 1106 28-29 -0.003572593154842707 1106 30-31 -0.22624107772781343 1106 32-33 -0.12212299948485139 1106 34-35 -0.3165807480119085 1106 36-37 -0.4422409788435715 1106 38-39 -0.3504464682147912 1106 40-41 -0.33522258331813504 1106 42-43 -0.5278013075547747 1106 44-45 -0.5068915876002436 1106 46-47 -0.6372701887530781 1106 48-49 -0.8487308639766766 1106 50-51 -0.6603048058503802 1106 52-53 -0.7161179096033834 1106 54-55 -0.4966317486946039 1106 56-57 -0.6713659791398001 1106 58-59 -0.6302708274119624 1106 60-61 -0.6230977954318959 1106 62-63 -0.5624714411409357 1106 64-65 -0.5251667906029951 1106 66-67 -0.6640134239912143 1106 68-69 -0.6952854341129147 1106 70-71 -0.6384078686749568 1106 72-73 -0.6769516493860763 1106 74-75 -0.33155201957466574 1106 76-77 -0.5649768823953565 1106 78-79 -0.7273547032848491 1106 80-81 -0.7886729379820814 1106 82-83 -0.5987893551788197 1106 84-85 -0.414469677516081 1106 86-87 -0.37887469540564567 1106 88-89 -0.27038748993360784 1106 90-91 -0.47807984109494583 1106 92-93 -0.3167543914700097 1106 94-95 -0.21333190604146424 1106 96-97 -0.09750896000225495 1106 98-99 0.16746405332012415 1106 100-101 0.32354827523795393 1107 1 -0.030779707569415393 1107 2 -0.012499985562229199 1107 3 -0.06830725371590063 1107 4 -0.09977524797461967 1107 5 -0.07523160217680669 1107 6 -0.18498331056469652 1107 7 -0.1052251824253645 1107 8 -0.05923187822438081 1107 9 -0.022150732848331245 1107 10-11 -0.16664297921028748 1107 12-13 -0.18573308138011413 1107 14-15 -0.13768943890816132 1107 16-17 -0.21963099128637253 1107 18-19 -0.14157028605873023 1107 20-21 -0.22585691770112248 1107 22-23 -0.11675091193294662 1107 24-25 -0.1844790785904209 1107 26-27 -0.14219020629646195 1107 28-29 -0.10747275900945397 1107 30-31 0.020682386204072145 1107 32-33 8.141001063464159E-4 1107 34-35 -0.06850806658092523 1107 36-37 -0.09677701635311564 1107 38-39 -0.02300891237994307 1107 40-41 -0.3300795600200601 1107 42-43 -0.393366772301583 1107 44-45 -0.15235918473614873 1107 46-47 -0.2968736871949673 1107 48-49 -0.20940246993520617 1107 50-51 -0.3478926829687552 1107 52-53 -0.4241812748642033 1107 54-55 -0.26917515879547693 1107 56-57 -0.20872343886697564 1107 58-59 -0.22998732506761854 1107 60-61 -0.10915558246845336 1107 62-63 -0.22332076213177743 1107 64-65 -0.20880294729064985 1107 66-67 -0.16540032817239592 1107 68-69 -0.06059312597915323 1107 70-71 0.027000062886578746 1107 72-73 -0.07536043987172292 1107 74-75 -0.11505532040661848 1107 76-77 0.03291559714063297 1107 78-79 0.0840225021319192 1107 80-81 0.15440707969377598 1107 82-83 0.19597130341893276 1107 84-85 0.47904908012384695 1107 86-87 0.459900562988949 1107 88-89 0.2985062579023463 1107 90-91 0.4032968095601497 1107 92-93 0.44897071073048167 1107 94-95 0.3706838797975749 1107 96-97 0.31162442574649063 1107 98-99 0.3627702913281823 1107 100-101 0.830501925856705 1108 1 0.209781000641307 1108 2 0.12076754277737223 1108 3 -0.05129107562027002 1108 4 -0.030388227699233994 1108 5 -0.13862167679718596 1108 6 -0.07978223285272179 1108 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-1.4073669011607777 2316 100-101 -1.4604399153849386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 17.0 5 32.0 6 130.0 7 292.0 8 612.0 9 989.0 10 1784.0 11 2690.0 12 3779.0 13 5045.0 14 5876.0 15 7542.0 16 9214.0 17 11943.0 18 11552.0 19 7819.0 20 7130.0 21 7314.0 22 7678.0 23 8552.0 24 9717.0 25 11220.0 26 13025.0 27 15374.0 28 18664.0 29 22409.0 30 26713.0 31 32310.0 32 39241.0 33 48836.0 34 64380.0 35 88954.0 36 128577.0 37 202019.0 38 174594.0 39 16196.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.16851485148515 21.03039603960396 15.24811881188119 22.552970297029702 2 15.934947462429413 22.620298984023364 38.82228114614494 22.622472407402284 3 18.208150732838845 26.201903128544163 32.08802759852523 23.501918540091758 4 13.1101886136325 19.033557656970565 37.043630486412155 30.81262324298478 5 12.731006363082068 37.949919510322346 34.877872107880876 14.441202018714716 6 29.306227777168537 37.57502289587637 18.019030005206528 15.099719321748564 7 25.458197165065577 34.64466503594052 22.3766779816405 17.520459817353405 8 24.669837230347312 35.41306899236141 21.26882481022062 18.64826896707066 9 25.40791205143505 17.155522273637956 21.94327760148899 35.493288073438 10-11 23.502560687908257 27.581187242399935 28.381945569575233 20.534306500116575 12-13 24.636741919804628 25.70092903970252 28.65308013609582 21.009248904397033 14-15 21.891016648423083 27.235118965292404 27.43230774094374 23.44155664534077 16-17 20.440072001722985 29.91089687985947 28.44186611710357 21.20716500131397 18-19 20.296810599983402 28.002584397981483 31.344667406947842 20.355937595087273 20-21 21.169390690634916 27.387851353647598 31.25610040425675 20.186657551460737 22-23 20.88121450898414 27.667333841782682 31.00823134708781 20.443220302145367 24-25 20.679305825930907 27.685310916698814 31.393184021923993 20.242199235446286 26-27 20.43350581029181 27.795596280425304 31.288736307840498 20.482161601442385 28-29 20.30268872230367 27.853457916595865 31.169163469099846 20.674689892000618 30-31 20.639569346036662 27.645599607993454 31.08840103217852 20.626430013791357 32-33 20.36398914078646 27.828512943724142 30.983977918018468 20.823519997470928 34-35 20.72279550483202 27.988491864129305 30.837071649705305 20.451640981333366 36-37 20.841810293293758 27.707579711419417 30.676631849787118 20.77397814549971 38-39 20.698218760127418 27.6926086647222 30.78206389857169 20.827108676578693 40-41 20.836087390813354 27.555938241928263 30.844258173005194 20.763716194253185 42-43 20.688869886444753 27.747454553448325 30.568280112493152 20.995395447613767 44-45 20.621886713416597 27.879042950264477 30.426775894739542 21.07229444157938 46-47 20.99657734030346 27.744981473971436 29.879290611914648 21.379150573810453 48-49 20.845653722395824 27.958992739656058 29.658786557348222 21.536566980599893 50-51 20.73088384862479 28.249260417505372 29.547092473868247 21.47276326000159 52-53 20.79264750629305 28.204268208348317 29.531785328997024 21.471298956361608 54-55 20.65058465088893 28.21222096207574 29.227160284046676 21.910034102988654 56-57 20.727504981659 28.339193430631692 28.98391939245989 21.94938219524942 58-59 20.60560676016042 28.234546782853016 29.183644537353125 21.976201919633443 60-61 20.552489080726062 28.14969556441853 29.323721276702948 21.97409407815246 62-63 20.374148282140524 28.1605067678042 29.33579590977842 22.129549040276856 64-65 20.328478294588194 28.23175556923296 29.099853489334755 22.33991264684409 66-67 20.263740280782873 28.281810367823585 28.8462906428762 22.608158708517344 68-69 20.27764564318919 28.030661152085184 28.946662576668935 22.745030628056693 70-71 20.227567419379536 27.80936103790897 28.840996712695517 23.12207483001598 72-73 20.132074987058253 27.780006085585462 28.686620010511465 23.40129891684482 74-75 19.99688803598023 27.90127664619192 28.557110714294538 23.544724603533314 76-77 19.813197002137095 27.628566683866225 28.89382140534214 23.664414908654543 78-79 19.674756049724454 27.612544586744992 28.75924814789716 23.953451215633397 80-81 19.707878844160888 27.5181112350415 28.642820659996822 24.1311892608008 82-83 19.585544030266192 27.350567095938278 28.725072699224523 24.338816174571004 84-85 19.43549279411358 27.153418004639274 28.672937322421433 24.73815187882572 86-87 19.30264893720122 27.138354704024337 28.646318525155312 24.91267783361913 88-89 19.416963180117918 26.773553610854723 28.607013018171507 25.202470190855852 90-91 19.19943707397479 26.67357927131031 28.720880528967978 25.40610312574692 92-93 19.052666884339924 26.544068214386098 28.713111421869048 25.69015347940493 94-95 18.88722259104704 26.29946533573596 28.846628809433483 25.96668326378351 96-97 18.96299889402224 25.995367637579136 28.85021287231662 26.191420596082004 98-99 18.698998644574147 26.07515302876427 28.75340338342745 26.472444943234137 100-101 18.74783890426766 25.571961771080932 29.178003157422612 26.502196167228796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2196.0 1 1448.5 2 797.5 3 870.5 4 773.0 5 797.0 6 1140.5 7 1858.5 8 2597.0 9 2745.0 10 2297.0 11 1881.5 12 1802.0 13 1845.0 14 1923.0 15 1999.5 16 2037.5 17 2182.0 18 2356.5 19 2555.0 20 2882.5 21 3277.0 22 3704.5 23 4181.0 24 4859.0 25 5798.0 26 7240.5 27 8964.5 28 10560.5 29 12123.0 30 13900.0 31 16233.5 32 18745.0 33 20628.0 34 22751.0 35 25129.5 36 27625.5 37 29779.5 38 30753.0 39 30900.5 40 31114.0 41 31899.5 42 32674.0 43 33820.5 44 35043.5 45 36200.0 46 36891.0 47 37063.5 48 37370.5 49 37175.5 50 36597.0 51 35289.5 52 33293.5 53 31472.5 54 29629.5 55 27167.5 56 23874.5 57 20678.0 58 18464.5 59 16175.0 60 13279.0 61 10671.0 62 8374.5 63 6203.0 64 4352.5 65 3079.0 66 2259.5 67 1801.0 68 1371.0 69 955.5 70 734.5 71 533.5 72 395.0 73 328.5 74 268.5 75 208.0 76 140.0 77 93.0 78 64.0 79 38.0 80 31.0 81 25.0 82 23.0 83 22.0 84 12.5 85 10.0 86 7.5 87 4.0 88 4.0 89 2.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22010851310179128 2 0.0 3 0.0 4 0.0 5 0.029341215615454226 6 0.0036553029554606273 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029637591530761844 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.457719011922215E-4 26-27 0.0015312755624226951 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0073600018968058584 36-37 0.01689342717253425 38-39 0.012250204499381561 40-41 0.008397317600382523 42-43 0.007656377812113476 44-45 0.0033589270401530092 46-47 3.457719011922215E-4 48-49 0.0016794635200765046 50-51 1.4818795765380924E-4 52-53 0.0 54-55 0.0 56-57 0.0014818795765380922 58-59 3.457719011922215E-4 60-61 0.002667383237768566 62-63 8.891277459228552E-4 64-65 0.0014818795765380922 66-67 0.005729934362613957 68-69 2.4697992942301536E-4 70-71 4.939598588460307E-5 72-73 0.0 74-75 5.433558447306339E-4 76-77 0.05670659179552432 78-79 0.14003761998284972 80-81 0.0010867116894612678 82-83 9.385237318074584E-4 84-85 0.0 86-87 0.10175573092228232 88-89 0.35179821147014306 90-91 0.38775848919413414 92-93 7.409397882690461E-4 94-95 3.951678870768246E-4 96-97 1.4818795765380924E-4 98-99 0.0 100-101 9.879197176920615E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1012228.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.44049236998674 #Duplication Level Percentage of deduplicated Percentage of total 1 84.33183874914573 58.56044405207007 2 10.167297330012879 14.120442653362916 3 2.467837330275385 5.14103517910069 4 1.0042781215611913 2.7895026895045807 5 0.5197148768995437 1.8044628471955675 6 0.3344860720522225 1.3936126520525516 7 0.2170864912456661 1.0552214987280308 8 0.16467941468484565 0.9148335711133526 9 0.12371458560116978 0.773172156374469 >10 0.6200573786662261 7.634387034002581 >50 0.03405733112543213 1.592954002090537 >100 0.012958677173742503 1.4669642909399228 >500 7.120148413972643E-4 0.32177283883815727 >1k 9.9682077795617E-4 1.3172513786610611 >5k 2.8480593655890574E-4 1.1139431559655322 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5996 0.5923566627281601 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5269 0.5205348992519472 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3571 0.35278613118783514 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2952 0.29163390066269657 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1973 0.19491656030064372 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1446 0.1428531911782721 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1193 0.11785882232066293 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1179 0.11647573471589405 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.879197176920614E-5 0.0 14-15 0.0 4.939598588460307E-5 0.0 2.4697992942301536E-4 0.0 16-17 0.0 9.879197176920614E-5 0.0 2.963759153076184E-4 0.0 18-19 0.0 9.879197176920614E-5 0.0 3.951678870768246E-4 0.0 20-21 0.0 9.879197176920614E-5 0.0 9.385237318074583E-4 0.0 22-23 0.0 9.879197176920614E-5 0.0 0.0017288595059611076 0.0 24-25 0.0 9.879197176920614E-5 0.0 0.004248054786075864 0.0 26-27 0.0 9.879197176920614E-5 0.0 0.009780405205151409 0.0 28-29 0.0 9.879197176920614E-5 0.0 0.024500408998763126 0.0 30-31 0.0 9.879197176920614E-5 0.0 0.06707974883129098 0.0 32-33 0.0 9.879197176920614E-5 0.0 0.14986742117388574 0.0 34-35 0.0 9.879197176920614E-5 0.0 0.28457027468119833 0.0 36-37 0.0 9.879197176920614E-5 0.0 0.49386106687426157 0.0 38-39 0.0 9.879197176920614E-5 0.0 0.7637113377618481 0.0 40-41 0.0 9.879197176920614E-5 0.0 1.04660214892297 0.0 42-43 0.0 9.879197176920614E-5 0.0 1.335568666347898 0.0 44-45 0.0 9.879197176920614E-5 0.0 1.6930968121806549 0.0 46-47 0.0 9.879197176920614E-5 0.0 2.0957234931260547 0.0 48-49 0.0 9.879197176920614E-5 0.0 2.507488431460106 0.0 50-51 0.0 9.879197176920614E-5 0.0 2.94128397949869 0.0 52-53 0.0 9.879197176920614E-5 0.0 3.3673243577533913 0.0 54-55 0.0 9.879197176920614E-5 0.0 3.8368825995724283 0.0 56-57 0.0 9.879197176920614E-5 0.0 4.385079250919754 0.0 58-59 0.0 9.879197176920614E-5 0.0 4.972150543158261 0.0 60-61 0.0 9.879197176920614E-5 0.0 5.5823885527766475 0.0 62-63 0.0 9.879197176920614E-5 0.0 6.181265485641575 0.0 64-65 0.0 9.879197176920614E-5 0.0 6.81649786411757 0.0 66-67 0.0 9.879197176920614E-5 0.0 7.531603551768969 0.0 68-69 0.0 9.879197176920614E-5 0.0 8.330830603381848 0.0 70-71 0.0 9.879197176920614E-5 0.0 9.178070553274559 0.0 72-73 0.0 9.879197176920614E-5 0.0 10.039487151116152 0.0 74-75 0.0 9.879197176920614E-5 0.0 10.909597442473435 0.0 76-77 0.0 9.879197176920614E-5 0.0 11.822583449578554 0.0 78-79 0.0 9.879197176920614E-5 0.0 12.80551417269627 0.0 80-81 0.0 9.879197176920614E-5 0.0 13.839767325148088 0.0 82-83 0.0 9.879197176920614E-5 0.0 14.891704240546595 0.0 84-85 0.0 9.879197176920614E-5 0.0 15.961374314877677 0.0 86-87 0.0 9.879197176920614E-5 0.0 17.06250963221725 0.0 88-89 0.0 9.879197176920614E-5 0.0 18.200296771083195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3410 0.0 46.161705 1 GTATCAA 5485 0.0 34.767704 1 TATCAAC 6810 0.0 27.876638 2 ATCAACG 6865 0.0 27.4459 3 TCAACGC 6900 0.0 27.306683 4 CAACGCA 6980 0.0 26.993711 5 AACGCAG 7095 0.0 26.482744 6 TATTCCG 150 1.0677195E-6 25.311985 5 CGCAGAG 8025 0.0 23.235193 8 ACGCAGA 8045 0.0 23.118452 7 GTACATG 3800 0.0 22.776974 1 GTGGTAT 1865 0.0 22.18445 1 TACATGG 3855 0.0 21.79096 2 TACACCG 110 0.0028912378 21.572716 5 GAGTACT 5090 0.0 21.487944 12-13 GCAGAGT 8460 0.0 21.479649 9 ACATGGG 3815 0.0 21.148613 3 TGGTATC 1900 0.0 20.982304 2 AGACCCG 115 0.0037412005 20.634771 5 AGTACTT 5280 0.0 19.995754 12-13 >>END_MODULE