FastQCFastQC Report
Thu 26 May 2016
SRR938783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865446
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127380.3295350652938885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96150.2487423184801961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63340.16386207438934602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109900.033.8598181
GTATCAA164650.031.3817671
TATCAAC199850.025.556552
ATCAACG200850.025.311363
CAACGCA204800.024.846315
TCAACGC204650.024.8413724
AACGCAG210950.024.1278446
GTACATG114700.023.0199531
TACATGG111100.022.900652
ACGCAGA233850.021.6242147
CGCAGAG232750.021.5840118
ACATGGG114400.021.3289173
GAGTACT134050.021.26425612-13
AGAGTAC195350.019.76617610-11
GCAGAGT250900.019.4756139
AGTACTT139700.019.37047812-13
GTACTTT146150.019.08294714-15
CATGGGG65800.018.5052454
CAGAGTA239100.017.27817710-11
TATTGCG2504.6779296E-617.0565075
Produced by FastQC (version 0.11.5)