##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938783_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3865446 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.622938724276576 33.0 31.0 34.0 30.0 34.0 2 31.689812249349753 33.0 31.0 34.0 30.0 34.0 3 31.705500477823257 33.0 31.0 34.0 28.0 34.0 4 35.272162643068874 37.0 35.0 37.0 33.0 37.0 5 35.30797144753801 37.0 35.0 37.0 33.0 37.0 6 35.39108656543126 37.0 35.0 37.0 33.0 37.0 7 35.302456171939795 37.0 35.0 37.0 32.0 37.0 8 35.27944640799535 37.0 35.0 37.0 32.0 37.0 9 36.855858547758785 39.0 37.0 39.0 33.0 39.0 10-11 36.756362137771426 39.0 37.0 39.0 32.0 39.0 12-13 36.64675046553489 39.0 37.0 39.0 32.0 39.0 14-15 37.81619844126654 40.0 37.5 41.0 32.0 41.0 16-17 37.75355495847051 40.0 37.0 41.0 32.0 41.0 18-19 37.69920689617705 40.0 37.0 41.0 32.0 41.0 20-21 37.58624580449448 40.0 37.0 41.0 32.0 41.0 22-23 37.495885985731015 40.0 37.0 41.0 32.0 41.0 24-25 37.49595389510033 40.0 37.0 41.0 32.0 41.0 26-27 37.40016274965424 40.0 37.0 41.0 31.0 41.0 28-29 37.27930347494183 40.0 37.0 41.0 31.0 41.0 30-31 37.07429336744065 39.5 36.5 41.0 30.5 41.0 32-33 36.86106545014469 39.0 36.0 41.0 30.0 41.0 34-35 36.53201778009575 39.0 35.5 41.0 30.0 41.0 36-37 36.28102526849425 39.0 35.0 41.0 30.0 41.0 38-39 36.1016771415252 39.0 35.0 41.0 29.0 41.0 40-41 35.91978066696573 39.0 35.0 41.0 28.5 41.0 42-43 35.65280047890981 39.0 35.0 40.0 27.0 41.0 44-45 35.625999431889625 39.0 35.0 41.0 27.0 41.0 46-47 35.454468255409594 39.0 35.0 41.0 26.5 41.0 48-49 35.2287461783194 39.0 35.0 41.0 25.5 41.0 50-51 34.06408393753269 38.0 33.5 40.0 23.5 40.5 52-53 34.14600553209125 38.0 33.5 39.5 24.0 40.5 54-55 34.67465643033171 38.0 34.0 40.0 24.0 41.0 56-57 34.57959624840187 38.0 34.0 40.0 24.0 41.0 58-59 34.353779744950515 38.0 34.0 40.0 23.0 41.0 60-61 34.06859855240508 38.0 34.0 40.0 23.0 41.0 62-63 33.69986982097279 37.0 33.0 40.0 22.0 41.0 64-65 33.29910636444022 36.5 33.0 39.5 21.5 41.0 66-67 32.901325228705815 36.0 32.5 39.0 20.0 41.0 68-69 32.492546008921096 35.5 32.0 39.0 18.5 40.5 70-71 32.085643804104365 35.0 31.5 38.5 17.5 40.0 72-73 31.66942508057285 35.0 31.0 37.5 14.0 40.0 74-75 31.212534594973 35.0 31.0 37.0 9.5 39.0 76-77 30.772996958177657 35.0 31.0 36.5 7.0 39.0 78-79 30.34855486275064 35.0 30.0 36.0 4.0 38.5 80-81 29.913649679752346 34.0 30.0 35.5 2.0 37.0 82-83 29.44823236438952 34.0 29.0 35.0 2.0 37.0 84-85 29.031035875290975 34.0 29.0 35.0 2.0 36.5 86-87 28.636499643249447 34.0 29.0 35.0 2.0 36.0 88-89 28.24634738138885 34.0 28.5 35.0 2.0 36.0 90-91 27.823579478280127 34.0 27.0 35.0 2.0 35.0 92-93 27.363126402490167 34.0 26.5 35.0 2.0 35.0 94-95 26.847051543340665 33.5 25.5 35.0 2.0 35.0 96-97 26.255528598769715 33.0 24.5 35.0 2.0 35.0 98-99 25.543478035910994 33.0 21.5 35.0 2.0 35.0 100-101 23.378603400487293 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3442466250476208 1101 2 0.16440122255560397 1101 3 0.2896536247043393 1101 4 0.3092259517346534 1101 5 0.07019936877004795 1101 6 0.051664214109329976 1101 7 0.23994851459063682 1101 8 0.2951607872178954 1101 9 0.43328580897395597 1101 10-11 0.4177403092005463 1101 12-13 0.288710738898871 1101 14-15 0.5217358715961637 1101 16-17 0.6492469037687059 1101 18-19 0.5914305795484509 1101 20-21 0.5609380742080745 1101 22-23 0.5655908626061716 1101 24-25 0.4710341170569521 1101 26-27 0.5230071231626994 1101 28-29 0.4181179166940936 1101 30-31 0.46225348001917865 1101 32-33 0.3747284696556008 1101 34-35 0.34409110463147385 1101 36-37 0.507909497714671 1101 38-39 0.6108392763552075 1101 40-41 0.4914659752114332 1101 42-43 0.4269139627269638 1101 44-45 0.5109518388565135 1101 46-47 0.4974777007415909 1101 48-49 0.40128840805855504 1101 50-51 0.40866120786813553 1101 52-53 0.4918684790087937 1101 54-55 0.3828509130091291 1101 56-57 0.3642369813185553 1101 58-59 0.39706756312580893 1101 60-61 0.260338206132964 1101 62-63 0.29125671433403255 1101 64-65 0.3411822517825769 1101 66-67 0.27529808983470616 1101 68-69 0.2379461639956162 1101 70-71 0.09597017555832821 1101 72-73 0.10053258226857764 1101 74-75 0.10929375736811053 1101 76-77 -0.02861328367847804 1101 78-79 -0.17098982074486813 1101 80-81 -0.33940460401255734 1101 82-83 -0.3069400251052308 1101 84-85 -0.29699044686044473 1101 86-87 -0.38829520743997037 1101 88-89 -0.5175307830321252 1101 90-91 -0.6207606674905719 1101 92-93 -0.7024498485648998 1101 94-95 -0.9089850360189082 1101 96-97 -1.1251971207294744 1101 98-99 -1.258127718335274 1101 100-101 -1.0507573215021928 1102 1 0.34815844374766414 1102 2 0.32602182172107064 1102 3 0.30819703736117177 1102 4 0.21399724954256527 1102 5 0.18033406504974892 1102 6 0.23469526074411107 1102 7 0.23327621825574596 1102 8 0.2092613815051365 1102 9 0.38834193165391184 1102 10-11 0.3072869422863036 1102 12-13 0.3533398229105842 1102 14-15 0.32920252705277164 1102 16-17 0.4503231312627278 1102 18-19 0.4430066710742935 1102 20-21 0.39933757000168413 1102 22-23 0.359012065575115 1102 24-25 0.4686279574361478 1102 26-27 0.4417488060824155 1102 28-29 0.41211160271935654 1102 30-31 0.5306922141075887 1102 32-33 0.4403425146307356 1102 34-35 0.46246280932765416 1102 36-37 0.5268277083055395 1102 38-39 0.4615367331187059 1102 40-41 0.5744757874028679 1102 42-43 0.5373409983155142 1102 44-45 0.6713890682097485 1102 46-47 0.6568853906589709 1102 48-49 0.6589235686011108 1102 50-51 0.5229684183706027 1102 52-53 0.6189111970421806 1102 54-55 0.5378058371672054 1102 56-57 0.5043179401360476 1102 58-59 0.39786581805184795 1102 60-61 0.44860803169640207 1102 62-63 0.3750259004004235 1102 64-65 0.5226903160975311 1102 66-67 0.36827415481364767 1102 68-69 0.29389853724877923 1102 70-71 0.2682601201023971 1102 72-73 0.2960354382165171 1102 74-75 0.270072370049089 1102 76-77 0.39495065047411515 1102 78-79 0.15631048232715017 1102 80-81 0.06440074990047862 1102 82-83 -0.05589901383449458 1102 84-85 -0.1887569954827022 1102 86-87 -0.3041408294952106 1102 88-89 -0.3200663203128151 1102 90-91 -0.23814694927025215 1102 92-93 -0.3439396872909235 1102 94-95 -0.5045737536463939 1102 96-97 -0.4693680273555145 1102 98-99 -0.4952321440613794 1102 100-101 -0.32753658769280136 1103 1 0.16062668463112217 1103 2 0.2906174748272292 1103 3 0.19443343164255822 1103 4 0.2948940712032666 1103 5 0.22069441736823592 1103 6 0.17055036468482143 1103 7 0.18868649402062232 1103 8 0.17549893381626447 1103 9 0.3047664122820919 1103 10-11 0.26409017101799037 1103 12-13 0.38570999616570134 1103 14-15 0.42011819082244983 1103 16-17 0.402573252418577 1103 18-19 0.4464428378510803 1103 20-21 0.3679039985384307 1103 22-23 0.4348552484439949 1103 24-25 0.4056290283120916 1103 26-27 0.40445723258838484 1103 28-29 0.3377138460401099 1103 30-31 0.36062114606338724 1103 32-33 0.3205983939688579 1103 34-35 0.3514498258238561 1103 36-37 0.38014100028554765 1103 38-39 0.36062680521510515 1103 40-41 0.5224411231093526 1103 42-43 0.4107044142861369 1103 44-45 0.599317624409025 1103 46-47 0.45415381260520604 1103 48-49 0.5152482656662585 1103 50-51 0.5549428560702623 1103 52-53 0.48939522030289595 1103 54-55 0.5926268597496929 1103 56-57 0.542780586288302 1103 58-59 0.4259331870354046 1103 60-61 0.5080558203873338 1103 62-63 0.4229778313094741 1103 64-65 0.1951465786108173 1103 66-67 0.22039956159421337 1103 68-69 0.09136290446242867 1103 70-71 0.03988496852565504 1103 72-73 0.16555752778468857 1103 74-75 0.1061669056867025 1103 76-77 0.024657596571749707 1103 78-79 0.1935418925570751 1103 80-81 0.03578218589958482 1103 82-83 0.10541196630800798 1103 84-85 0.1548370644278485 1103 86-87 0.09609098755297651 1103 88-89 0.08020311762357224 1103 90-91 -0.09401258102825238 1103 92-93 -0.04499423320604734 1103 94-95 -0.01829685848095508 1103 96-97 0.004026239251277985 1103 98-99 -0.015957936188407018 1103 100-101 0.12847795945273432 1104 1 0.2585768178464285 1104 2 0.23016723313040188 1104 3 0.21423347850657493 1104 4 0.2891315329092876 1104 5 0.22054688250227628 1104 6 0.21163882485474517 1104 7 0.19762971045427236 1104 8 0.22461502639873032 1104 9 0.28374269388275764 1104 10-11 0.2652986551406329 1104 12-13 0.38532814121850834 1104 14-15 0.4581062491768293 1104 16-17 0.40181388178495325 1104 18-19 0.39502042817279204 1104 20-21 0.4121709672176479 1104 22-23 0.4889137590361372 1104 24-25 0.3612080158757678 1104 26-27 0.47341459501212313 1104 28-29 0.49410080395795575 1104 30-31 0.4167191092143909 1104 32-33 0.4653821686050108 1104 34-35 0.2875672288630753 1104 36-37 0.5712160087471077 1104 38-39 0.6155865802678306 1104 40-41 0.531189072808651 1104 42-43 0.6043092117728364 1104 44-45 0.7215177164013511 1104 46-47 0.6169390105188839 1104 48-49 0.7452638002197958 1104 50-51 0.7922765539015089 1104 52-53 0.7154186349539771 1104 54-55 0.7395412114207502 1104 56-57 0.705873871716193 1104 58-59 0.7196013377292587 1104 60-61 0.634139550763031 1104 62-63 0.65678154050957 1104 64-65 0.5324503355427836 1104 66-67 0.5701027561579863 1104 68-69 0.6351460455665077 1104 70-71 0.6555805086334345 1104 72-73 0.7110481679992233 1104 74-75 0.4801374160705514 1104 76-77 0.2907931377209607 1104 78-79 0.3667499948247759 1104 80-81 0.44330603013747805 1104 82-83 0.45802246558335113 1104 84-85 0.5612674149533312 1104 86-87 0.44386971996812363 1104 88-89 0.3269052794432845 1104 90-91 0.3184146278724356 1104 92-93 0.32589540203569456 1104 94-95 0.5371675725985661 1104 96-97 0.3297940714433878 1104 98-99 0.24145352119571584 1104 100-101 0.41473465276245847 1105 1 0.1113456560155015 1105 2 0.14051615035966947 1105 3 0.24317707700882352 1105 4 0.2913920822414937 1105 5 0.28342341424942674 1105 6 0.25246297794605255 1105 7 0.2788164038242158 1105 8 0.23247655464958683 1105 9 0.22022716099316852 1105 10-11 0.25712529346836277 1105 12-13 0.285295773607686 1105 14-15 0.4223062389683605 1105 16-17 0.2536139953739607 1105 18-19 0.35053371721519255 1105 20-21 0.362165168260681 1105 22-23 0.39424850427690217 1105 24-25 0.41536030303695526 1105 26-27 0.32452747280733263 1105 28-29 0.4106330075349689 1105 30-31 0.3460453020915111 1105 32-33 0.3931107506380087 1105 34-35 0.3790361820537882 1105 36-37 0.3438109372406757 1105 38-39 0.4002972252738175 1105 40-41 0.37661026174384205 1105 42-43 0.3182877361222296 1105 44-45 0.45567254344089037 1105 46-47 0.5205023921826211 1105 48-49 0.5331405821392039 1105 50-51 0.47105375365537583 1105 52-53 0.5659575563940606 1105 54-55 0.6033323153528087 1105 56-57 0.5049350454207655 1105 58-59 0.44987878272393544 1105 60-61 0.6183051586071073 1105 62-63 0.6059707268190806 1105 64-65 0.5519400143712758 1105 66-67 0.44100793122493087 1105 68-69 0.3148364280593725 1105 70-71 0.2291203539470743 1105 72-73 0.23923444647917336 1105 74-75 0.1647125649014498 1105 76-77 0.19668373332459055 1105 78-79 0.06966316618902724 1105 80-81 0.1849752039261361 1105 82-83 0.19220598144164214 1105 84-85 -0.026626234695644513 1105 86-87 -0.007509787461057016 1105 88-89 0.008364757764656616 1105 90-91 0.11820950821774545 1105 92-93 0.13617232004881075 1105 94-95 0.2484776408477174 1105 96-97 0.2173213393197102 1105 98-99 0.2454525697492187 1105 100-101 0.4019651407481071 1106 1 -0.049207785267622484 1106 2 0.07977345707582728 1106 3 0.0499440012027641 1106 4 0.044044650444348576 1106 5 0.07792287375477258 1106 6 0.013265270480864899 1106 7 0.09635586394907847 1106 8 0.05608177299709638 1106 9 0.14534018589607456 1106 10-11 0.14236647683758719 1106 12-13 -0.01445500945705902 1106 14-15 -0.00483948464197681 1106 16-17 0.09973263000618005 1106 18-19 0.07413079014053636 1106 20-21 0.09996470970782667 1106 22-23 0.0773186243157582 1106 24-25 0.01404840248911654 1106 26-27 -0.08079049120424031 1106 28-29 0.05122449998568612 1106 30-31 0.01801706931255609 1106 32-33 0.027062576775691127 1106 34-35 0.01977907467102824 1106 36-37 0.12028900870298997 1106 38-39 0.08590350424707083 1106 40-41 0.1437973893381539 1106 42-43 0.19517290660677844 1106 44-45 0.011182089789635086 1106 46-47 0.09234422701353395 1106 48-49 0.00862035180731624 1106 50-51 0.0488595647831076 1106 52-53 -0.0356604212983882 1106 54-55 -0.04750580986928554 1106 56-57 -0.08212352790853572 1106 58-59 -0.12457467809098688 1106 60-61 -0.07821437276725618 1106 62-63 -0.2051986331428992 1106 64-65 -0.13043157835416963 1106 66-67 -0.2366886480706114 1106 68-69 -0.049673831300623306 1106 70-71 -0.13136648990546718 1106 72-73 -0.2120831042382676 1106 74-75 -0.2731267858625124 1106 76-77 -0.41653942743764816 1106 78-79 -0.10499038806880279 1106 80-81 -0.11766308110685131 1106 82-83 -0.15298159415088364 1106 84-85 -0.27862243674915277 1106 86-87 -0.17439231498555685 1106 88-89 -0.2452850868843619 1106 90-91 -0.19736106856705504 1106 92-93 -0.0883587158739374 1106 94-95 -0.13093952981694557 1106 96-97 0.05893181261175684 1106 98-99 0.023161273964561246 1106 100-101 0.054126466857255195 1107 1 0.09646158376193625 1107 2 0.05187662046983377 1107 3 0.04351792545879718 1107 4 0.09131528083970153 1107 5 0.04409259432267021 1107 6 0.09331410245455629 1107 7 -0.11564557603315251 1107 8 -0.03318119741448555 1107 9 0.11034196473082147 1107 10-11 0.05319019290512017 1107 12-13 0.06665005850231864 1107 14-15 0.13032883935740358 1107 16-17 0.14556778868894327 1107 18-19 0.2633334042100728 1107 20-21 0.144626358989278 1107 22-23 0.28303599933077095 1107 24-25 0.18983378198984013 1107 26-27 0.21745391730642183 1107 28-29 0.010597013916125775 1107 30-31 0.13802787481741774 1107 32-33 0.30886382878217233 1107 34-35 0.25152903365351875 1107 36-37 0.35127802954325915 1107 38-39 0.3652172066872055 1107 40-41 0.323388093841281 1107 42-43 0.4035163181242609 1107 44-45 0.44920196199544193 1107 46-47 0.4255580316732406 1107 48-49 0.4643934739808415 1107 50-51 0.5792003258086496 1107 52-53 0.5302126341758893 1107 54-55 0.45329614577767785 1107 56-57 0.41491235832046414 1107 58-59 0.43848585923550587 1107 60-61 0.3347285105688158 1107 62-63 0.30381203873792373 1107 64-65 0.31915205873422536 1107 66-67 0.38751693730134207 1107 68-69 0.4109910159366592 1107 70-71 0.4138598900727999 1107 72-73 0.32882473859054784 1107 74-75 0.3342848799105056 1107 76-77 0.33756612878509173 1107 78-79 0.28967680069008495 1107 80-81 0.252518348760546 1107 82-83 0.43513612806109236 1107 84-85 0.27435949355999156 1107 86-87 0.36163085046073107 1107 88-89 0.30888444818856087 1107 90-91 0.445069609030849 1107 92-93 0.5147487900193362 1107 94-95 0.5459009580404626 1107 96-97 0.5559395081158449 1107 98-99 0.3746436550433998 1107 100-101 0.5240695864315974 1108 1 0.19524164477378392 1108 2 0.08241545552385432 1108 3 0.07023363311117237 1108 4 0.012751014972799624 1108 5 0.10002580959069718 1108 6 0.1303115416038807 1108 7 0.10763113181913297 1108 8 0.17835105483933233 1108 9 0.1606645495701713 1108 10-11 0.24052621205272118 1108 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2316 100-101 -2.8138625547794938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10475.0 3 2870.0 4 2942.0 5 3433.0 6 4222.0 7 5341.0 8 6601.0 9 8438.0 10 10680.0 11 14024.0 12 18370.0 13 23113.0 14 24954.0 15 24012.0 16 28297.0 17 34557.0 18 30324.0 19 24490.0 20 24950.0 21 27168.0 22 30499.0 23 34320.0 24 39547.0 25 45737.0 26 52809.0 27 62379.0 28 72368.0 29 86337.0 30 102269.0 31 122721.0 32 150456.0 33 188075.0 34 246930.0 35 340211.0 36 499880.0 37 721747.0 38 632585.0 39 107292.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.828091812406505 19.246391172095272 14.320417846418069 23.60509916908015 2 15.516839120298739 20.851201677140597 39.512472257187106 24.119486945373556 3 18.61896393554005 24.569885944200202 30.85223755112884 25.958912569130906 4 12.992943065428788 16.804620026155977 37.53481205246734 32.6676248559479 5 13.541481842004993 37.107620211255075 34.87488217630181 14.476015770438122 6 31.459287359927284 36.25104363845103 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21.756410674628263 27.05490322176432 29.493621040237233 21.695065063370183 30-31 21.989201961731204 26.63901383802011 29.23361833945053 22.13816586079816 32-33 21.678948773642155 26.76264229166373 29.188115218893973 22.37029371580014 34-35 22.39450833999418 26.8417311463874 28.930618518810185 21.833141994808237 36-37 22.438877311452956 26.439935201015263 29.10569993235485 22.015487555176932 38-39 21.99346301196499 26.773169373736792 29.025643157894056 22.20772445640416 40-41 22.353699954599392 26.64692162452334 28.989696855147386 22.00968156572988 42-43 21.982368407887634 26.731281620146973 28.76500241364009 22.521347558325306 44-45 22.0664680034739 26.83910191692091 28.838477883526153 22.255952196079036 46-47 22.321915542961772 27.086160057854585 28.280781868591344 22.311142530592303 48-49 22.1174964134176 27.093715403029446 28.245970797329957 22.542817386223003 50-51 22.076415567463545 27.306312194263587 28.22030007247807 22.396972165794793 52-53 22.35022562332342 27.10006446380669 28.08672461477677 22.46298529809312 54-55 22.001545188310892 27.1829553643311 28.16154430460353 22.653955142754477 56-57 21.90193390426795 27.388269711582 28.044009077889008 22.665787306261038 58-59 22.012479597410554 26.977104283942992 28.101554071652142 22.908862046994315 60-61 21.798860980329874 27.012705728837272 28.451960220801613 22.736473070031245 62-63 21.83072348128804 26.92794552665287 28.56972640926683 22.671604582792256 64-65 21.676219657080225 27.066778730592674 28.263506554075867 22.99349505825123 66-67 21.587335631502597 27.060033172669463 28.152742464656548 23.199888731171388 68-69 21.79552329476104 26.744011453518052 28.31845418586279 23.14201106585812 70-71 21.757814744453547 26.637052825231187 28.094510368051058 23.510622062264204 72-73 21.75079052768007 26.640994041682358 28.08374686528332 23.524468565354255 74-75 21.51820453415564 27.077143033536633 27.90868001286746 23.495972419440264 76-77 21.594407771794756 26.707682335322648 28.069911429443223 23.62799846343937 78-79 21.671548163903978 26.816218384813382 28.00009406149751 23.51213938978513 80-81 21.73866100394107 26.599414004565887 27.9254329839994 23.736492007493652 82-83 21.63765714842608 26.543403826787515 28.080849819705684 23.738089205080726 84-85 21.579416244558463 26.49742836894583 28.04210920784512 23.88104617865059 86-87 21.57641266580889 26.396636083918047 28.029575784732035 23.99737546554103 88-89 21.651112348114864 26.225377780382907 27.862022123363005 24.26148774813922 90-91 21.487272574141507 26.123742126266713 28.0192868773538 24.36969842223798 92-93 21.611888960360645 25.95490936655041 27.983973574542492 24.449228098546453 94-95 21.550272509296487 25.724563036922472 28.085686369751915 24.639478084029125 96-97 21.58490245252348 25.352244305560284 28.16570137814553 24.897151863770706 98-99 21.51654136765125 25.47967457695498 27.96197800272477 25.041806052668996 100-101 21.720570238086108 24.932262881748645 28.239669252865014 25.107497627300233 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2932.0 1 1820.5 2 922.5 3 1145.5 4 1024.0 5 1037.5 6 1643.5 7 2924.0 8 4417.0 9 5029.5 10 4498.0 11 4164.5 12 4433.0 13 4663.0 14 5096.5 15 5512.0 16 5738.0 17 6163.5 18 6759.5 19 7219.5 20 7815.5 21 8821.0 22 10211.0 23 12174.5 24 14437.5 25 17851.0 26 23096.5 27 29752.5 28 36088.5 29 41957.0 30 48168.0 31 56695.0 32 66873.0 33 72476.5 34 75183.5 35 80562.5 36 88970.5 37 97170.5 38 102408.5 39 106137.5 40 110484.5 41 115117.0 42 118168.5 43 122469.5 44 127674.0 45 132891.5 46 135918.0 47 138754.5 48 143769.0 49 143457.5 50 140181.5 51 138299.0 52 136319.0 53 135794.0 54 133446.0 55 124910.0 56 114081.5 57 102336.0 58 94065.5 59 84774.5 60 69277.0 61 56017.0 62 46242.0 63 35175.5 64 25015.5 65 18332.5 66 13284.5 67 9912.0 68 7435.5 69 5564.0 70 4136.0 71 2747.0 72 1949.5 73 1477.5 74 1020.5 75 729.5 76 527.5 77 352.5 78 217.5 79 134.0 80 98.0 81 67.5 82 57.5 83 47.0 84 35.5 85 31.0 86 28.5 87 22.0 88 15.0 89 14.5 90 11.5 91 7.5 92 4.5 93 3.5 94 2.5 95 4.0 96 3.5 97 2.5 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16114569961655137 2 0.09403830761055774 3 0.08767422957143885 4 0.10337746278178507 5 0.1243064836502696 6 0.04035756805294913 7 0.04366895825216547 8 0.04853256260726447 9 0.04796341741677416 10-11 0.04674751632799941 12-13 0.0511583915542993 14-15 0.048351470955744824 16-17 0.04766590970356332 18-19 0.051843952806480804 20-21 0.05374541514743706 22-23 0.06669346823109158 24-25 0.08339270552479584 26-27 0.08476382802915886 28-29 0.07512716514472069 30-31 0.06285173819528199 32-33 0.05263299500238782 34-35 0.296382357947828 36-37 0.46989403033957783 38-39 0.44868043687584824 40-41 0.42371565920206883 42-43 0.4533370793434962 44-45 0.46296080710996873 46-47 0.4622623107398215 48-49 0.47779738741661376 50-51 0.46761744957761664 52-53 0.4740979436784268 54-55 0.4661428461295281 56-57 0.44482577172207294 58-59 0.4233276056630981 60-61 0.39563351809855835 62-63 0.45011623497003966 64-65 0.48718828305970385 66-67 0.48910268051862577 68-69 0.4990756564701718 70-71 0.4956478502092644 72-73 0.4865285920434537 74-75 0.4794660176341876 76-77 0.4628702612842089 78-79 0.4372069872402822 80-81 0.4429631147350138 82-83 0.4545659155502367 84-85 0.45270325856317745 86-87 0.46324537970521384 88-89 0.4743695811557062 90-91 0.4780302195399962 92-93 0.4798799414090896 94-95 0.48368286609100214 96-97 0.4851962748929878 98-99 0.4789874182694572 100-101 0.46658264014036155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3865446.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.79655702966749 #Duplication Level Percentage of deduplicated Percentage of total 1 73.95461274731079 36.08730478531125 2 13.601779641028038 13.274400319167897 3 4.83360296710492 7.075895485293156 4 2.35193583391227 4.590654841984746 5 1.3486826139134438 3.2905534042374196 6 0.8178006296126407 2.394351303707472 7 0.546541741809257 1.8668548691302447 8 0.41274463186881316 1.6112413574140523 9 0.29257341041918283 1.284891759619557 >10 1.641784382092405 14.670491167709136 >50 0.1246386169133944 4.167209628593662 >100 0.06655585384554252 5.950487933735895 >500 0.004728163686999243 1.5943398966529156 >1k 0.0018593901810858631 1.3976591715197264 >5k 1.0625086749062059E-4 0.4134520286853027 >10k+ 5.3125433745310296E-5 0.3302120472376567 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12738 0.3295350652938885 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9615 0.2487423184801961 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6334 0.16386207438934602 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 2.5870235931377646E-5 6 0.0 0.0 0.0 0.0 1.0348094372551059E-4 7 0.0 0.0 0.0 0.0 1.0348094372551059E-4 8 0.0 0.0 0.0 0.0 1.0348094372551059E-4 9 0.0 0.0 0.0 0.0 1.2935117965688823E-4 10-11 0.0 0.0 0.0 1.2935117965688823E-5 1.2935117965688823E-4 12-13 0.0 0.0 0.0 2.5870235931377646E-5 1.4228629762257707E-4 14-15 0.0 0.0 0.0 2.5870235931377646E-5 2.0696188745102117E-4 16-17 0.0 0.0 0.0 3.880535389706647E-5 2.5870235931377646E-4 18-19 0.0 0.0 0.0 5.174047186275529E-5 2.7163747727946533E-4 20-21 0.0 0.0 0.0 1.681565335539547E-4 2.8457259524515414E-4 22-23 0.0 0.0 0.0 4.0098865693635353E-4 3.751184210049759E-4 24-25 0.0 0.0 0.0 9.960040833580394E-4 4.6566424676479763E-4 26-27 0.0 0.0 0.0 0.001992008166716079 4.915344826961753E-4 28-29 0.0 0.0 0.0 0.00747649818416814 5.174047186275529E-4 30-31 0.0 0.0 0.0 0.027939854805887857 5.303398365932417E-4 32-33 0.0 0.0 0.0 0.07063867921062666 5.562100725246195E-4 34-35 0.0 0.0 0.0 0.1399321061528217 6.467558982844411E-4 36-37 0.0 0.0 0.0 0.25533922864269737 6.726261342158188E-4 38-39 0.0 0.0 0.0 0.4203654636489554 7.243666060785741E-4 40-41 0.0 0.0 0.0 0.5981457249693826 7.761070779413294E-4 42-43 0.0 0.0 0.0 0.7890680661429497 8.019773138727071E-4 44-45 0.0 0.0 0.0 1.0281737217387075 8.278475498040847E-4 46-47 0.0 0.0 0.0 1.3075463995616547 8.795880216668399E-4 48-49 0.0 0.0 0.0 1.590114051522127 9.313284935295953E-4 50-51 0.0 0.0 0.0 1.8929639684527995 9.571987294609729E-4 52-53 0.0 0.0 0.0 2.1990217946389627 9.830689653923506E-4 54-55 0.0 0.0 0.0 2.542940711110697 0.0010089392013237282 56-57 0.0 0.0 0.0 2.9387294506248436 0.0010477445552207947 58-59 0.0 0.0 0.0 3.359030238683971 0.0010736147911521723 60-61 0.0 0.0 0.0 3.804748533545676 0.001086549909117861 62-63 0.0 0.0 0.0 4.256235891020079 0.001086549909117861 64-65 0.0 0.0 0.0 4.740617253481228 0.001086549909117861 66-67 0.0 0.0 0.0 5.289700076006753 0.0010994850270835501 68-69 0.0 0.0 0.0 5.893511382645108 0.001112420145049239 70-71 0.0 0.0 0.0 6.531225633471532 0.0011382903809806165 72-73 1.2935117965688823E-5 0.0 0.0 7.178162623407493 0.0012288362067404382 74-75 2.5870235931377646E-5 0.0 0.0 7.860166718148436 0.001241771324706127 76-77 2.5870235931377646E-5 2.5870235931377646E-5 0.0 8.588905394099413 0.001241771324706127 78-79 2.5870235931377646E-5 2.5870235931377646E-5 0.0 9.385527569134325 0.0012676415606375046 80-81 2.5870235931377646E-5 2.5870235931377646E-5 0.0 10.221524243256795 0.0012805766786031935 82-83 2.5870235931377646E-5 2.5870235931377646E-5 0.0 11.078643965017232 0.0013452522684316377 84-85 2.5870235931377646E-5 2.5870235931377646E-5 0.0 11.9582966622739 0.0013452522684316377 86-87 2.5870235931377646E-5 2.5870235931377646E-5 0.0 12.868295146278076 0.0013581873863973265 88-89 2.5870235931377646E-5 2.5870235931377646E-5 0.0 13.82179443200086 0.0013711225043630153 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10990 0.0 33.859818 1 GTATCAA 16465 0.0 31.381767 1 TATCAAC 19985 0.0 25.55655 2 ATCAACG 20085 0.0 25.31136 3 CAACGCA 20480 0.0 24.84631 5 TCAACGC 20465 0.0 24.841372 4 AACGCAG 21095 0.0 24.127844 6 GTACATG 11470 0.0 23.019953 1 TACATGG 11110 0.0 22.90065 2 ACGCAGA 23385 0.0 21.624214 7 CGCAGAG 23275 0.0 21.584011 8 ACATGGG 11440 0.0 21.328917 3 GAGTACT 13405 0.0 21.264256 12-13 AGAGTAC 19535 0.0 19.766176 10-11 GCAGAGT 25090 0.0 19.475613 9 AGTACTT 13970 0.0 19.370478 12-13 GTACTTT 14615 0.0 19.082947 14-15 CATGGGG 6580 0.0 18.505245 4 CAGAGTA 23910 0.0 17.278177 10-11 TATTGCG 250 4.6779296E-6 17.056507 5 >>END_MODULE