FastQCFastQC Report
Thu 26 May 2016
SRR938783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3865446
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120940.3128746333540813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98860.25575315241759944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68640.17757329943297617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120900.032.3897931
GTATCAA176750.030.9417651
TATCAAC213550.025.5322362
ATCAACG213150.025.4910983
CAACGCA217750.024.9743885
TCAACGC218100.024.9125524
AACGCAG222300.024.505916
GTACATG117850.022.1520351
TACATGG114750.022.0829432
CGCAGAG247600.021.7718898
ACGCAGA248100.021.7662647
GAGTACT141500.021.2299712-13
ACATGGG115650.020.5160053
AGTACTT149850.019.84113312-13
GCAGAGT266850.019.8103439
AGAGTAC205450.019.65724810-11
GTACTTT153200.019.34531414-15
TAAGGTG15700.019.0418575
CATGGGG65250.017.6723444
CAGAGTA251250.017.53780210-11
Produced by FastQC (version 0.11.5)