##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938783_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3865446 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97621257676346 31.0 31.0 34.0 30.0 34.0 2 32.199071465491954 34.0 31.0 34.0 30.0 34.0 3 32.29279415622415 34.0 31.0 34.0 30.0 34.0 4 35.93807700327466 37.0 35.0 37.0 35.0 37.0 5 35.83838268598242 37.0 35.0 37.0 35.0 37.0 6 35.85911250603423 37.0 35.0 37.0 35.0 37.0 7 35.78738184416494 37.0 35.0 37.0 33.0 37.0 8 35.755450211954845 37.0 35.0 37.0 33.0 37.0 9 37.406871807289505 39.0 37.0 39.0 34.0 39.0 10-11 37.33495436231679 39.0 37.0 39.0 34.0 39.0 12-13 37.26699829722107 39.0 37.0 39.0 33.0 39.0 14-15 38.4971412095784 40.0 38.0 41.0 34.0 41.0 16-17 38.43506454365162 40.0 38.0 41.0 33.0 41.0 18-19 38.450160990478196 40.0 38.0 41.0 33.5 41.0 20-21 38.501924874904475 40.0 38.0 41.0 34.0 41.0 22-23 38.453374461834414 40.0 38.0 41.0 34.0 41.0 24-25 38.38228926235162 40.0 38.0 41.0 34.0 41.0 26-27 38.217735029799925 40.0 38.0 41.0 33.0 41.0 28-29 38.054311197207255 40.0 37.5 41.0 33.0 41.0 30-31 37.8295540799173 40.0 37.0 41.0 33.0 41.0 32-33 37.600918884910044 40.0 37.0 41.0 32.0 41.0 34-35 37.560474392864364 40.0 37.0 41.0 32.0 41.0 36-37 37.47422677745337 40.0 37.0 41.0 32.0 41.0 38-39 37.37090221930406 40.0 36.5 41.0 31.5 41.0 40-41 37.21328431441029 40.0 36.0 41.0 31.0 41.0 42-43 37.02848623418876 40.0 36.0 41.0 31.0 41.0 44-45 36.785125959591724 40.0 36.0 41.0 30.5 41.0 46-47 36.50685418965884 39.0 35.0 41.0 30.0 41.0 48-49 36.23516781763347 39.0 35.0 41.0 29.5 41.0 50-51 35.99562909428821 39.0 35.0 41.0 29.0 41.0 52-53 35.785543375848476 39.0 35.0 40.5 28.0 41.0 54-55 35.540880017467586 38.5 35.0 40.0 28.0 41.0 56-57 35.27252831885376 38.0 34.5 40.0 27.5 41.0 58-59 34.96293920546296 38.0 34.0 40.0 26.5 41.0 60-61 34.6167911801122 37.5 34.0 40.0 26.0 41.0 62-63 34.23852085891253 37.0 33.5 40.0 26.0 41.0 64-65 33.87141690764791 36.5 33.0 39.0 25.5 41.0 66-67 33.485250731739626 36.0 33.0 39.0 25.0 40.5 68-69 33.040207650035725 35.5 32.5 39.0 23.5 40.0 70-71 32.58017600038909 35.0 32.0 38.0 22.5 40.0 72-73 32.25195281475928 35.0 32.0 37.0 22.0 39.5 74-75 31.845457548753753 35.0 31.0 37.0 21.0 39.0 76-77 29.777875567269597 33.0 28.5 35.0 18.0 37.5 78-79 30.85244497012764 34.0 30.5 36.0 19.0 38.0 80-81 30.924271481221055 34.0 31.0 35.5 19.0 37.0 82-83 30.63705572397079 34.0 31.0 35.0 18.0 37.0 84-85 30.27420134701144 34.0 30.5 35.0 15.0 36.5 86-87 29.77425645061398 34.0 30.0 35.0 7.5 36.0 88-89 29.282950014047536 34.0 29.5 35.0 2.0 35.5 90-91 28.897731076827874 34.0 29.0 35.0 2.0 35.0 92-93 28.638899366334442 34.0 29.0 35.0 2.0 35.0 94-95 28.28667869115233 34.0 29.0 35.0 2.0 35.0 96-97 27.81026173952501 33.5 28.5 35.0 2.0 35.0 98-99 27.126938909507466 33.0 27.0 35.0 2.0 35.0 100-101 24.16688126027372 30.0 20.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06237621059971943 1101 2 0.12813595748566797 1101 3 0.14308899142778841 1101 4 0.12178371684033351 1101 5 0.15967662284603534 1101 6 0.2099500320477361 1101 7 0.11567935998207446 1101 8 0.11577738052708497 1101 9 0.26787720569409856 1101 10-11 0.1855116154271883 1101 12-13 0.19829503611169486 1101 14-15 0.303306487656414 1101 16-17 0.25581113041089765 1101 18-19 0.23224952603612792 1101 20-21 0.28817877855388474 1101 22-23 0.23581749867131663 1101 24-25 0.21975508798519883 1101 26-27 0.29004956072244426 1101 28-29 0.30729196437409456 1101 30-31 0.16889953810252933 1101 32-33 0.21282256872081717 1101 34-35 0.1795424939041439 1101 36-37 0.3167984853149548 1101 38-39 0.3085957439640765 1101 40-41 0.2873591490810625 1101 42-43 0.27569341403331293 1101 44-45 0.300689638668473 1101 46-47 0.30681331418181657 1101 48-49 0.20581929504531615 1101 50-51 0.29936975821433975 1101 52-53 0.32544773395641613 1101 54-55 0.3050648924947481 1101 56-57 0.22126263251311684 1101 58-59 0.17389009957412327 1101 60-61 0.27647731039089507 1101 62-63 0.11838803533216691 1101 64-65 0.1809129192680814 1101 66-67 0.13469002358495175 1101 68-69 0.12066498180229956 1101 70-71 0.16182515004287978 1101 72-73 0.16289639035446868 1101 74-75 0.17126604630243847 1101 76-77 0.023859947916175628 1101 78-79 0.11718692069161563 1101 80-81 0.2550445389481091 1101 82-83 0.31485258175227315 1101 84-85 0.21443497495791064 1101 86-87 0.3052495341297643 1101 88-89 0.45395279273668976 1101 90-91 0.4571173954946737 1101 92-93 0.42111696569097745 1101 94-95 0.4312468185837375 1101 96-97 0.307810721659024 1101 98-99 0.3007028567838077 1101 100-101 0.8698303082162298 1102 1 0.13914711543953828 1102 2 0.13190256893354757 1102 3 0.1103218893857445 1102 4 0.10630320710281893 1102 5 0.09249463211774156 1102 6 0.15078354323300402 1102 7 0.15145485447975204 1102 8 0.11822542389400326 1102 9 0.1930080077093237 1102 10-11 0.22600172608958502 1102 12-13 0.16069020740851414 1102 14-15 0.20143720990773772 1102 16-17 0.18410469420338416 1102 18-19 0.23313509009469868 1102 20-21 0.19435572259874334 1102 22-23 0.13634814417684282 1102 24-25 0.11703073508137862 1102 26-27 0.14872656405128026 1102 28-29 0.1427407348551739 1102 30-31 0.16782826890491975 1102 32-33 0.18061874147033308 1102 34-35 0.06044598482198893 1102 36-37 0.102227591993163 1102 38-39 0.09432150151323526 1102 40-41 0.13086284147204452 1102 42-43 -0.031189871672658853 1102 44-45 -0.05881079602937689 1102 46-47 -0.12293940541297133 1102 48-49 0.026524139978448602 1102 50-51 -0.05787226485682595 1102 52-53 0.01930480505684784 1102 54-55 0.021743061793159768 1102 56-57 -0.06162973580639175 1102 58-59 -0.14249278931414722 1102 60-61 -0.1269804238277885 1102 62-63 -0.2046361220423094 1102 64-65 -0.17797096204940033 1102 66-67 -0.21297463420174267 1102 68-69 -0.17894000211710903 1102 70-71 -0.13447910211205993 1102 72-73 -0.1406973274531751 1102 74-75 -0.03641227605642783 1102 76-77 -0.10561911067271623 1102 78-79 -0.2680190472494566 1102 80-81 -0.16065040941015596 1102 82-83 -0.07885940528347746 1102 84-85 -0.10751129189144137 1102 86-87 -0.11776436788629141 1102 88-89 0.08317616029458819 1102 90-91 0.07799519566177793 1102 92-93 0.07395098625651286 1102 94-95 0.07508106920191082 1102 96-97 0.1309189544944651 1102 98-99 0.2228081740793577 1102 100-101 0.7046400436002642 1103 1 0.008920639200407976 1103 2 0.06703691998603745 1103 3 -0.05735478629686952 1103 4 0.012595530602048655 1103 5 -0.0029914041243657152 1103 6 0.03605430958633349 1103 7 0.09502020490286611 1103 8 0.06203851427430607 1103 9 0.11953388530135811 1103 10-11 0.08220195003566033 1103 12-13 0.12314848139534007 1103 14-15 0.13919092472542616 1103 16-17 0.04765855781582928 1103 18-19 0.021702531907472178 1103 20-21 0.11590765133648517 1103 22-23 -0.013594542397889597 1103 24-25 -0.009731754525752478 1103 26-27 0.06684496475188695 1103 28-29 0.1207744335207579 1103 30-31 0.1132604899366001 1103 32-33 0.09034753331659573 1103 34-35 -0.14265381050228143 1103 36-37 -0.028948001203040974 1103 38-39 0.10470366037621659 1103 40-41 0.06756302063492825 1103 42-43 0.09923292679669515 1103 44-45 0.06306791422705516 1103 46-47 0.1083346655471118 1103 48-49 0.049552571719686966 1103 50-51 -0.015365863274432456 1103 52-53 -0.0416905667445846 1103 54-55 -0.051604778074242574 1103 56-57 1.4388986997460051E-5 1103 58-59 -0.2886424007258057 1103 60-61 -0.1547274498174147 1103 62-63 -0.03920991432133292 1103 64-65 -0.2274683650772218 1103 66-67 -0.19055002148579803 1103 68-69 -0.10953994294903424 1103 70-71 -0.12745575725773506 1103 72-73 -0.06852131416960816 1103 74-75 -0.04447008224748217 1103 76-77 -0.021702213782301527 1103 78-79 -0.09295970576427592 1103 80-81 -0.03510128768826348 1103 82-83 -0.05395038148747133 1103 84-85 -0.17547421805166508 1103 86-87 0.0810803466578669 1103 88-89 0.21481850883922604 1103 90-91 0.326799168065687 1103 92-93 0.291136524636304 1103 94-95 0.35731960704460164 1103 96-97 0.37740211767684073 1103 98-99 0.5079702092804474 1103 100-101 0.9841347445586877 1104 1 -0.013508999686308698 1104 2 0.054939060977304166 1104 3 0.0312268829299569 1104 4 0.04467568542687417 1104 5 0.06905334233262295 1104 6 0.05287328430579663 1104 7 0.01867091176844582 1104 8 -0.004491031752230867 1104 9 0.009737570201600931 1104 10-11 0.09614833412965851 1104 12-13 0.04789919086447014 1104 14-15 0.04513744767577066 1104 16-17 0.18702693501908385 1104 18-19 0.15127936719780877 1104 20-21 0.09796046881849918 1104 22-23 0.11619057740910677 1104 24-25 0.11326893107744951 1104 26-27 0.1469477184467678 1104 28-29 0.18538602628981238 1104 30-31 0.04925639367444745 1104 32-33 0.034366730206215834 1104 34-35 0.13466617526494673 1104 36-37 0.05433976989227318 1104 38-39 0.16990322297873206 1104 40-41 0.10455955790484239 1104 42-43 0.11460041879075078 1104 44-45 0.03219624352487216 1104 46-47 0.003649999626802014 1104 48-49 0.030872054132657922 1104 50-51 0.06959903210599094 1104 52-53 -0.043300432487860974 1104 54-55 -0.05775134094579215 1104 56-57 -0.010874985899093303 1104 58-59 -0.0939614665697448 1104 60-61 -0.004413287338131511 1104 62-63 -0.20324264970453498 1104 64-65 -0.04591803399162586 1104 66-67 -0.14450829515428865 1104 68-69 -0.016848993779127852 1104 70-71 -0.01667226033426772 1104 72-73 -0.08966653187032847 1104 74-75 -0.003264462036941751 1104 76-77 -0.06517075848102394 1104 78-79 -0.007973114080034094 1104 80-81 -0.0696786870825008 1104 82-83 -0.10489547248176834 1104 84-85 -0.13049994986799263 1104 86-87 0.06477557433887782 1104 88-89 0.30017610731874456 1104 90-91 0.2589027548986351 1104 92-93 0.263794842565261 1104 94-95 0.3246775179508994 1104 96-97 0.25287401228487383 1104 98-99 0.2916276853928217 1104 100-101 0.932323971042063 1105 1 0.02297021920606923 1105 2 0.04597202331417094 1105 3 0.05537221350254384 1105 4 0.07788905846236815 1105 5 0.04083254368054412 1105 6 0.07442359063543336 1105 7 0.03034657707726751 1105 8 0.024575601106981537 1105 9 0.10347859144723515 1105 10-11 0.08545053638705724 1105 12-13 0.031522698820921846 1105 14-15 0.12290783187341958 1105 16-17 -0.04335731013273403 1105 18-19 0.00667792976201298 1105 20-21 0.06212316674525198 1105 22-23 0.027809973757477735 1105 24-25 -0.007111188211887054 1105 26-27 -0.009003908389239257 1105 28-29 0.01984257907158593 1105 30-31 0.03507144661485739 1105 32-33 0.06483367978711385 1105 34-35 0.03839675838803913 1105 36-37 0.06701572828059454 1105 38-39 0.1270813274130873 1105 40-41 0.1404156858905239 1105 42-43 0.11888549162760143 1105 44-45 0.16628108712473733 1105 46-47 0.25206946888869197 1105 48-49 0.30625987692776135 1105 50-51 0.28650252011201616 1105 52-53 0.23355638741309548 1105 54-55 0.2425793759816699 1105 56-57 0.1882938086716095 1105 58-59 0.2591512774642979 1105 60-61 0.21333812264351337 1105 62-63 0.13976050736138035 1105 64-65 0.114306211062285 1105 66-67 0.24177052316010617 1105 68-69 0.15405656284550417 1105 70-71 0.12900301002634507 1105 72-73 -0.047924012614792844 1105 74-75 0.0032353732093284293 1105 76-77 -0.052882549095986775 1105 78-79 -0.09070230840513815 1105 80-81 -0.022925343467271375 1105 82-83 0.0010803040763214256 1105 84-85 0.03445978607680544 1105 86-87 0.04840761334933674 1105 88-89 0.0733570868262916 1105 90-91 0.17679074813484164 1105 92-93 0.05458452947845771 1105 94-95 0.19801199163778094 1105 96-97 0.14569125356662127 1105 98-99 0.376157472053837 1105 100-101 0.6261802947020456 1106 1 -0.19377638158593413 1106 2 -0.24359587064390098 1106 3 -0.18797908564927468 1106 4 -0.06730481262371057 1106 5 -0.09435658355754839 1106 6 -0.20566098121794596 1106 7 -0.10037411209427916 1106 8 -0.11074330794564702 1106 9 -0.14233031637498073 1106 10-11 -0.19879626732346622 1106 12-13 -0.12654459613313662 1106 14-15 -0.08027226713935676 1106 16-17 -0.1990152523065447 1106 18-19 -0.25501261532448183 1106 20-21 -0.22849129923771017 1106 22-23 -0.31583882690080856 1106 24-25 -0.2301850826787657 1106 26-27 -0.19866949005125178 1106 28-29 -0.16867396746444996 1106 30-31 -0.12277116805419297 1106 32-33 -0.25351075024988035 1106 34-35 -0.20528401448633105 1106 36-37 -0.28270402904318104 1106 38-39 -0.1595833975455463 1106 40-41 -0.16600695798158682 1106 42-43 -0.19464797673618506 1106 44-45 -0.15341300208083197 1106 46-47 -0.2664444088743352 1106 48-49 -0.20007831555142985 1106 50-51 -0.2339778031006361 1106 52-53 -0.214666870864761 1106 54-55 -0.15920447147408368 1106 56-57 -0.3347709293655896 1106 58-59 -0.3389877947204951 1106 60-61 -0.2635430396623377 1106 62-63 -0.24260435965455684 1106 64-65 -0.25078311897961214 1106 66-67 -0.302394694395808 1106 68-69 -0.4032689967722405 1106 70-71 -0.31000254865066523 1106 72-73 -0.17973382933993065 1106 74-75 -0.10774130736478682 1106 76-77 -0.307248524061432 1106 78-79 -0.27548899012362327 1106 80-81 -0.3444541603360918 1106 82-83 -0.18601186911944723 1106 84-85 -0.3021998695037702 1106 86-87 -0.1539745067606475 1106 88-89 0.01143226763888805 1106 90-91 -0.0233559339966547 1106 92-93 0.03600432675496279 1106 94-95 -0.03495185071847118 1106 96-97 0.0861989112672994 1106 98-99 0.050380595243883164 1106 100-101 0.5279043062880504 1107 1 -0.09310603433821996 1107 2 -0.053534567093507235 1107 3 -0.051563181118851276 1107 4 -0.039621238851687224 1107 5 -0.055475794803612644 1107 6 -0.020899111090365352 1107 7 0.015366291923911035 1107 8 0.006988066019538053 1107 9 0.0355314509692235 1107 10-11 -0.00923175482285643 1107 12-13 -0.04454440218094646 1107 14-15 -0.08764301407516939 1107 16-17 -0.05995364824302385 1107 18-19 -0.06166018559409281 1107 20-21 0.026734274485825438 1107 22-23 -0.06438749790546439 1107 24-25 -0.08453016139572611 1107 26-27 -0.0840130194059725 1107 28-29 -0.0069673178926379364 1107 30-31 -0.057276714015507935 1107 32-33 0.014675438775697103 1107 34-35 0.06914804107420736 1107 36-37 0.09213507239437746 1107 38-39 0.0629902690472619 1107 40-41 0.04359848181345427 1107 42-43 -0.03777159644781847 1107 44-45 -0.035513118057473037 1107 46-47 -0.043243710082649045 1107 48-49 -0.3145965811678195 1107 50-51 -0.21017729025496124 1107 52-53 -0.18161994120118408 1107 54-55 -0.22455761950413944 1107 56-57 -0.15468124903149771 1107 58-59 -0.18710984138967746 1107 60-61 -0.17359862895279576 1107 62-63 -0.19603278876024177 1107 64-65 -0.18575324808749372 1107 66-67 -0.1913647490801722 1107 68-69 -0.06735141968611202 1107 70-71 -0.0885683511588482 1107 72-73 -0.057685962052836715 1107 74-75 -0.067347310974327 1107 76-77 -0.019395005323339376 1107 78-79 -0.06160955452449102 1107 80-81 -0.08715677976186953 1107 82-83 0.0018598608269364547 1107 84-85 -0.010679653653024701 1107 86-87 -0.04818077716720737 1107 88-89 0.12723518458957628 1107 90-91 0.3473105877696838 1107 92-93 0.3914959222541441 1107 94-95 0.27954112781767293 1107 96-97 0.30503646108735794 1107 98-99 0.23699149501852546 1107 100-101 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quality scores pass #Quality Count 2 4.0 3 16.0 4 32.0 5 86.0 6 237.0 7 671.0 8 1483.0 9 2970.0 10 4693.0 11 7215.0 12 10034.0 13 13254.0 14 15628.0 15 19694.0 16 24546.0 17 32023.0 18 29816.0 19 22430.0 20 21128.0 21 21963.0 22 23562.0 23 26246.0 24 31061.0 25 36747.0 26 44166.0 27 54698.0 28 66990.0 29 82189.0 30 100101.0 31 121235.0 32 149090.0 33 187668.0 34 247638.0 35 342293.0 36 520858.0 37 819309.0 38 708311.0 39 75358.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.607579255734954 20.081937197929626 14.873743997548377 23.436739548787045 2 15.8958888573272 21.508695244998897 38.761503847162786 23.83391205051112 3 18.638366698176615 24.834883219167985 31.03101168662038 25.495738396035023 4 13.316703945676645 17.501706142059675 36.99078967860371 32.19080023365997 5 13.430119059999804 37.47158801338836 34.83572141481216 14.262571511799674 6 31.401214762798396 36.787941158665774 17.4184557228325 14.392388355703325 7 27.262287456609148 33.32321807108416 21.62071336658176 17.79378110572493 8 25.51777983704856 35.02165080045097 20.629676368522546 18.830892993977926 9 25.89380371631113 16.628947862678718 20.996645665209137 36.48060275580101 10-11 24.45108533400803 26.58452608056095 28.14075012301297 20.82363846241805 12-13 25.332367856128375 24.210673231497736 28.247943445594636 22.209015466779253 14-15 22.43568226476763 25.74055957687595 26.514805500074573 25.308952658281846 16-17 21.664078076049588 28.70106907261795 27.507661318436227 22.12719153289623 18-19 21.609896099850936 27.31990287268571 29.704533142652252 21.365667884811103 20-21 22.332823258382927 26.592397511800066 29.67237924740877 21.402399982408234 22-23 22.112157303452175 26.52550313728351 29.500192215852973 21.862147343411344 24-25 22.081733905996874 26.664322823291286 29.571218948602567 21.682724322109273 26-27 21.740180429466836 26.818251667854977 29.56195976451864 21.879608138159547 28-29 21.772933834801986 27.189501547816214 29.44093644045215 21.59662817692965 30-31 22.013721055733285 26.78813777245886 29.188145430048696 22.009995741759166 32-33 21.781328726361718 26.83091420757139 29.14870625537131 22.239050810695584 34-35 22.3998365909287 26.931579436898446 28.945435832768563 21.723148139404287 36-37 22.51235706543662 26.511756257062153 29.12096456394948 21.854922113551748 38-39 22.015052759950795 26.829806581129056 29.014383032572656 22.14075762634749 40-41 22.346672820779236 26.72193121847807 29.03377241753653 21.897623543206166 42-43 22.048403714845087 26.778641516414304 28.783413458584416 22.38954131015619 44-45 22.117242957427194 26.875627446970423 28.8676248423015 22.139504753300884 46-47 22.36809103158846 27.1033262288205 28.267738505118572 22.260844234472465 48-49 22.14058721326709 27.11257749433183 28.261100147874345 22.485735144526735 50-51 22.142067265746277 27.344386956223104 28.14039164341246 22.373154134618158 52-53 22.404646190892333 27.184456851809596 28.00293937620652 22.407957581091548 54-55 22.111730444559306 27.250594109968162 27.992591281833974 22.645084163638558 56-57 21.962704950476606 27.449937212937392 27.89127050280873 22.696087333777268 58-59 22.022202713101535 27.087630776251586 27.978243550488646 22.911922960158236 60-61 21.813749743721086 27.21232570608479 28.216085325841256 22.757839224352868 62-63 21.775197480445982 27.147734051905008 28.326588962826023 22.750479504822987 64-65 21.595308794897146 27.28906315079812 28.003676677930567 23.11195137637416 66-67 21.573975047330187 27.30332038253241 27.85863189249852 23.264072677638886 68-69 21.773729340834038 27.034962610741665 27.984770069966615 23.206537978457682 70-71 21.771712455392276 26.957229361700147 27.708646693375677 23.562411489531897 72-73 21.769467223187185 26.999251832776867 27.694579099022466 23.536701845013486 74-75 21.566657508603146 27.399296226101722 27.442279623101705 23.591766642193424 76-77 21.616235718985738 27.035608567559795 27.736559422407275 23.611596291047192 78-79 21.684300150293826 27.132195810276528 27.632002039286018 23.55150200014363 80-81 21.771022253450475 27.029769272390464 27.508304023430156 23.6909044507289 82-83 21.676437854777948 26.93812046527102 27.643498318175965 23.74194336177507 84-85 21.54460313247165 26.914306913096187 27.58781263533367 23.953277319098497 86-87 21.570400946818168 26.852143088103002 27.518859154579257 24.058596810499566 88-89 21.498719049093474 26.749359005470286 27.404680045170032 24.34724190026621 90-91 21.33334647756442 26.705739623072745 27.4955349609091 24.46537893845374 92-93 21.390843385213504 26.559755329656653 27.50169061991812 24.547710665211724 94-95 21.319975485345406 26.347665713865137 27.59139120664824 24.740967594141214 96-97 21.282970007334214 26.03115553319217 27.715903343599507 24.96997111587411 98-99 21.15271905396405 26.24346629078827 27.48700049114789 25.116814164099793 100-101 21.185544860443404 25.865151557876874 27.88733749829579 25.06196608338393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1350.0 1 970.0 2 782.0 3 978.5 4 866.5 5 887.5 6 1418.5 7 2602.5 8 3878.5 9 4369.5 10 3849.5 11 3338.5 12 3442.5 13 3604.0 14 3858.0 15 4169.5 16 4551.0 17 5026.5 18 5422.0 19 5990.0 20 6842.5 21 7914.0 22 9444.0 23 11570.0 24 14045.0 25 17477.5 26 22959.0 27 29802.0 28 36035.0 29 42339.5 30 48505.0 31 56873.5 32 67721.5 33 73420.0 34 75844.0 35 81392.0 36 90015.5 37 97822.5 38 102963.0 39 106660.0 40 111267.0 41 116577.5 42 120042.5 43 124103.5 44 129666.5 45 135148.5 46 137689.5 47 141105.5 48 146189.5 49 145683.5 50 142722.5 51 140517.0 52 137567.0 53 136289.5 54 134151.0 55 125354.5 56 113251.0 57 101378.0 58 93413.0 59 83845.0 60 68062.0 61 54970.0 62 45451.0 63 34232.0 64 23895.0 65 17203.5 66 12294.0 67 9306.5 68 6986.0 69 5144.5 70 3810.0 71 2442.5 72 1644.5 73 1280.5 74 929.5 75 598.5 76 389.0 77 225.0 78 124.5 79 68.0 80 28.0 81 16.5 82 10.0 83 9.0 84 10.5 85 9.0 86 6.0 87 5.5 88 5.0 89 1.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3863978438710565 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9402676948533236E-4 16-17 0.0022377754080641667 18-19 1.0348094372551059E-4 20-21 2.5870235931377646E-5 22-23 0.0 24-25 0.0 26-27 5.174047186275529E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007748135661447606 36-37 0.016893264063189604 38-39 0.011395838927771854 40-41 0.00724366606078574 42-43 0.0037123788561526927 44-45 0.002056683756544523 46-47 0.001616889745711103 48-49 5.174047186275529E-5 50-51 4.268588928677312E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.5272912879910877E-4 60-61 7.373017240442629E-4 62-63 0.0 64-65 0.0 66-67 0.001241771324706127 68-69 8.01977313872707E-4 70-71 0.002173099818235722 72-73 0.0 74-75 0.0 76-77 0.09734969780977408 78-79 0.03488601315346276 80-81 1.2935117965688823E-5 82-83 0.0 84-85 0.0 86-87 0.14958170415522556 88-89 0.3220585671097203 90-91 0.2787905975144912 92-93 0.0 94-95 7.761070779413294E-5 96-97 2.5870235931377646E-5 98-99 2.9750771321084295E-4 100-101 6.467558982844411E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3865446.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.071012328500686 #Duplication Level Percentage of deduplicated Percentage of total 1 72.44793048672216 34.1019742911486 2 14.307738567254372 13.469594769843907 3 5.179679830758781 7.31438319513999 4 2.4727776606143395 4.6558459099367475 5 1.4120382409347252 3.323303472367644 6 0.8821035745149148 2.4912904938603653 7 0.5760183320708704 1.8979636207245238 8 0.423268707177343 1.593894922705061 9 0.30014068674622746 1.2715133369504705 >10 1.7815922336376067 15.261386758258212 >50 0.13728022413955165 4.443020110314261 >100 0.07207600757349487 6.232985557391802 >500 0.004940226199988353 1.595725250120506 >1k 0.0022505474518613386 1.6018488201619319 >5k 1.0978280252982122E-4 0.43278546579960536 >10k+ 5.489140126491061E-5 0.31248402527644337 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12094 0.3128746333540813 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9886 0.25575315241759944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6864 0.17757329943297617 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.5870235931377646E-5 5 0.0 0.0 0.0 0.0 2.5870235931377646E-5 6 0.0 0.0 0.0 0.0 2.5870235931377646E-5 7 0.0 0.0 0.0 0.0 2.5870235931377646E-5 8 0.0 0.0 0.0 0.0 2.5870235931377646E-5 9 0.0 0.0 0.0 0.0 2.5870235931377646E-5 10-11 0.0 0.0 0.0 1.2935117965688823E-5 2.5870235931377646E-5 12-13 0.0 0.0 0.0 5.174047186275529E-5 1.681565335539547E-4 14-15 0.0 0.0 0.0 5.174047186275529E-5 1.9402676948533236E-4 16-17 0.0 0.0 0.0 6.467558982844412E-5 2.0696188745102117E-4 18-19 0.0 0.0 0.0 9.054582575982176E-5 2.0696188745102117E-4 20-21 0.0 0.0 0.0 1.9402676948533236E-4 2.4576724134808765E-4 22-23 0.0 0.0 0.0 4.1392377490204234E-4 3.4924818507359824E-4 24-25 0.0 0.0 0.0 0.001021874319289417 3.880535389706647E-4 26-27 0.0 0.0 0.0 0.0020308135206131453 3.880535389706647E-4 28-29 0.0 0.0 0.0 0.007618784481790717 4.0098865693635353E-4 30-31 0.0 0.0 0.0 0.028521935114343852 4.1392377490204234E-4 32-33 0.0 0.0 0.0 0.07225556895633777 4.1392377490204234E-4 34-35 0.0 0.0 0.0 0.14299772911068995 4.1392377490204234E-4 36-37 0.0 0.0 0.0 0.2601381574079679 4.1392377490204234E-4 38-39 0.0 0.0 0.0 0.4276479350636382 4.3979401083342E-4 40-41 0.0 0.0 0.0 0.608351533044311 4.3979401083342E-4 42-43 0.0 0.0 0.0 0.8018738329289816 4.656642467647977E-4 44-45 0.0 0.0 0.0 1.0437864091232938 5.432749545589307E-4 46-47 0.0 0.0 0.0 1.3274664812288155 6.079505443873747E-4 48-49 0.0 0.0 0.0 1.6133972638603669 6.467558982844411E-4 50-51 0.0 0.0 0.0 1.919623246580084 6.467558982844411E-4 52-53 0.0 0.0 0.0 2.22921235997088 7.243666060785741E-4 54-55 0.0 0.0 0.0 2.5772705141916354 8.278475498040847E-4 56-57 0.0 0.0 0.0 2.976719892090072 8.666529037011511E-4 58-59 0.0 0.0 0.0 3.4020912463917488 8.7958802166684E-4 60-61 0.0 0.0 0.0 3.853061199147524 9.183933755639065E-4 62-63 0.0 0.0 0.0 4.309748474044134 9.571987294609729E-4 64-65 0.0 2.5870235931377646E-5 0.0 4.799937704471877 9.701338474266618E-4 66-67 0.0 2.5870235931377646E-5 0.0 5.35394880694233 9.960040833580394E-4 68-69 0.0 2.5870235931377646E-5 0.0 5.9638913594964205 0.0010089392013237282 70-71 0.0 2.5870235931377646E-5 0.0 6.609069173389047 0.001021874319289417 72-73 0.0 2.5870235931377646E-5 0.0 7.264750303069814 0.0011382903809806165 74-75 0.0 2.5870235931377646E-5 0.0 7.9575552213121075 0.0011382903809806165 76-77 0.0 2.5870235931377646E-5 0.0 8.69501216677196 0.001177095734877683 78-79 0.0 2.5870235931377646E-5 0.0 9.49892974833952 0.0011900308528433718 80-81 0.0 2.5870235931377646E-5 0.0 10.341109408849587 0.0012029659708090606 82-83 0.0 2.5870235931377646E-5 0.0 11.204955391952183 0.0012159010887747494 84-85 0.0 2.5870235931377646E-5 0.0 12.090946297012039 0.001241771324706127 86-87 0.0 2.5870235931377646E-5 0.0 13.007257118583471 0.001306446914534571 88-89 0.0 2.5870235931377646E-5 0.0 13.97022491065714 0.0013711225043630153 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12090 0.0 32.389793 1 GTATCAA 17675 0.0 30.941765 1 TATCAAC 21355 0.0 25.532236 2 ATCAACG 21315 0.0 25.491098 3 CAACGCA 21775 0.0 24.974388 5 TCAACGC 21810 0.0 24.912552 4 AACGCAG 22230 0.0 24.50591 6 GTACATG 11785 0.0 22.152035 1 TACATGG 11475 0.0 22.082943 2 CGCAGAG 24760 0.0 21.771889 8 ACGCAGA 24810 0.0 21.766264 7 GAGTACT 14150 0.0 21.22997 12-13 ACATGGG 11565 0.0 20.516005 3 AGTACTT 14985 0.0 19.841133 12-13 GCAGAGT 26685 0.0 19.810343 9 AGAGTAC 20545 0.0 19.657248 10-11 GTACTTT 15320 0.0 19.345314 14-15 TAAGGTG 1570 0.0 19.041857 5 CATGGGG 6525 0.0 17.672344 4 CAGAGTA 25125 0.0 17.537802 10-11 >>END_MODULE