FastQCFastQC Report
Thu 26 May 2016
SRR938782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864700
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126600.3275804072761146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96800.2504722229409786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64360.16653297798017958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109600.033.8248561
GTATCAA166800.032.483341
TATCAAC203650.026.3479082
ATCAACG202750.026.3478683
CAACGCA206400.025.8819275
TCAACGC207300.025.8153344
AACGCAG212150.025.1104186
TACATGG117050.022.941032
GTACATG120700.022.7206341
ACGCAGA234850.022.5840727
CGCAGAG234500.022.4357078
GAGTACT129800.021.95797312-13
ACATGGG121700.020.8560073
GCAGAGT247300.020.6408659
AGTACTT136850.020.34122712-13
AGAGTAC194350.020.15850310-11
GTACTTT140500.019.88086914-15
CATGGGG72500.017.9300084
CAGAGTA238300.017.75509610-11
TACTTTT169150.016.09261514-15
Produced by FastQC (version 0.11.5)