##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938782_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3864700 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.578936786813983 33.0 31.0 34.0 30.0 34.0 2 31.649324915258624 33.0 31.0 34.0 30.0 34.0 3 31.666499340181645 33.0 31.0 34.0 28.0 34.0 4 35.23070044246643 37.0 35.0 37.0 33.0 37.0 5 35.26218464563873 37.0 35.0 37.0 33.0 37.0 6 35.3201086759645 37.0 35.0 37.0 33.0 37.0 7 35.22939115584651 37.0 35.0 37.0 32.0 37.0 8 35.201993945196264 37.0 35.0 37.0 32.0 37.0 9 36.78561699485083 39.0 37.0 39.0 32.0 39.0 10-11 36.668030636271894 39.0 37.0 39.0 32.0 39.0 12-13 36.56295831500505 39.0 37.0 39.0 32.0 39.0 14-15 37.73223199730897 40.0 37.0 41.0 32.0 41.0 16-17 37.62407172613657 40.0 37.0 41.0 32.0 41.0 18-19 37.57099749010273 40.0 37.0 41.0 32.0 41.0 20-21 37.45895671074081 40.0 37.0 41.0 32.0 41.0 22-23 37.45572903459518 40.0 37.0 41.0 32.0 41.0 24-25 37.39142585452946 40.0 37.0 41.0 31.5 41.0 26-27 37.256508137759724 40.0 37.0 41.0 31.0 41.0 28-29 37.08414042487127 40.0 36.5 41.0 30.5 41.0 30-31 36.85980308950242 39.5 36.0 41.0 30.0 41.0 32-33 36.67111664553523 39.0 36.0 41.0 30.0 41.0 34-35 36.37557481822651 39.0 35.0 41.0 30.0 41.0 36-37 36.16067663725516 39.0 35.0 41.0 29.5 41.0 38-39 35.9878022873703 39.0 35.0 41.0 29.0 41.0 40-41 35.876844127616636 39.0 35.0 40.5 28.0 41.0 42-43 35.87336533236733 39.0 35.0 41.0 27.5 41.0 44-45 35.702817941884234 39.0 35.0 41.0 27.0 41.0 46-47 35.41412321784356 39.0 35.0 41.0 26.0 41.0 48-49 35.13475845473128 39.0 35.0 41.0 25.0 41.0 50-51 33.978805470023545 38.0 33.0 40.0 23.5 40.5 52-53 34.03423008254198 38.0 33.5 39.5 23.5 40.5 54-55 34.53521618754367 38.0 34.0 40.0 24.0 41.0 56-57 34.421303852821694 38.0 34.0 40.0 23.0 41.0 58-59 34.208750614536704 38.0 34.0 40.0 23.0 41.0 60-61 33.900348021838695 37.5 33.5 40.0 22.0 41.0 62-63 33.49822871115481 37.0 33.0 40.0 21.5 41.0 64-65 33.0953080446089 36.5 32.5 39.5 20.0 41.0 66-67 32.71029950578311 36.0 32.0 39.0 18.5 41.0 68-69 32.274745775868766 35.0 32.0 39.0 17.0 40.5 70-71 31.843381892514294 35.0 31.0 38.5 13.0 40.0 72-73 31.415533935363676 35.0 31.0 37.5 9.5 40.0 74-75 30.942508862266152 35.0 31.0 37.0 7.0 39.0 76-77 30.470718813879472 35.0 30.0 36.5 4.0 39.0 78-79 30.01796970010609 34.0 30.0 36.0 2.0 38.5 80-81 29.539972831008875 34.0 29.0 35.5 2.0 37.0 82-83 29.10886472429943 34.0 29.0 35.0 2.0 37.0 84-85 28.740255776645018 34.0 29.0 35.0 2.0 36.5 86-87 28.29170453075271 34.0 28.5 35.0 2.0 36.0 88-89 27.809389732708876 34.0 27.0 35.0 2.0 36.0 90-91 27.331419903226642 34.0 26.0 35.0 2.0 35.0 92-93 26.84087924030326 33.0 25.0 35.0 2.0 35.0 94-95 26.293205035319687 33.0 24.5 35.0 2.0 35.0 96-97 25.662921442802805 33.0 21.5 35.0 2.0 35.0 98-99 24.862386601806094 32.5 10.0 35.0 2.0 35.0 100-101 22.445740937200817 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16750289251517714 1101 2 0.18066403307615886 1101 3 0.30904584578594907 1101 4 0.08112868638328052 1101 5 0.13449467757310174 1101 6 0.1399857012597181 1101 7 0.22713323341022829 1101 8 0.23519871014141813 1101 9 0.3436169362915109 1101 10-11 0.19693812639899733 1101 12-13 0.19069004535543144 1101 14-15 0.2403730298340676 1101 16-17 0.39706571608765984 1101 18-19 0.416798522353929 1101 20-21 0.5381940929796514 1101 22-23 0.39172917607439217 1101 24-25 0.41792270918934804 1101 26-27 0.3966182726477143 1101 28-29 0.4359688876978822 1101 30-31 0.4134255756247214 1101 32-33 0.39022564613367194 1101 34-35 0.4364154077603217 1101 36-37 0.5463657257940184 1101 38-39 0.43523744835443523 1101 40-41 0.6022446771128713 1101 42-43 0.7427937012022454 1101 44-45 0.7088001226257958 1101 46-47 0.7111664227938945 1101 48-49 0.6515550970780524 1101 50-51 0.6921955270527533 1101 52-53 0.6807139760037515 1101 54-55 0.6895753445814989 1101 56-57 0.659482485411111 1101 58-59 0.6813187339227298 1101 60-61 0.6195264302806933 1101 62-63 0.5131959913265973 1101 64-65 0.5629103599194423 1101 66-67 0.5100697837933055 1101 68-69 0.439413824485122 1101 70-71 0.4143821775502019 1101 72-73 0.39194930681920326 1101 74-75 0.20773820911875873 1101 76-77 0.2608176050553652 1101 78-79 0.181673343559428 1101 80-81 0.14283435557539192 1101 82-83 0.15508201537537403 1101 84-85 0.08200781006785718 1101 86-87 -0.11480166289164728 1101 88-89 0.053088825396372386 1101 90-91 -0.07929662236945845 1101 92-93 -0.17996180341648937 1101 94-95 -0.33966441541422654 1101 96-97 -0.35770524064890097 1101 98-99 -0.42075110606891286 1101 100-101 0.044183033915739856 1102 1 0.24107104948385683 1102 2 0.1774453436598762 1102 3 0.17451331376558699 1102 4 0.2232529180324434 1102 5 0.2849941773985307 1102 6 0.2732705245505116 1102 7 0.27492474158299984 1102 8 0.2800017326659301 1102 9 0.38639887453401656 1102 10-11 0.4053594640415312 1102 12-13 0.4156884589808456 1102 14-15 0.41827126440732343 1102 16-17 0.5758150298550433 1102 18-19 0.554001583181126 1102 20-21 0.5418289006507564 1102 22-23 0.41943018337200044 1102 24-25 0.37317542512005275 1102 26-27 0.4587984494832611 1102 28-29 0.4837239307898429 1102 30-31 0.4808337180762834 1102 32-33 0.49376896090714695 1102 34-35 0.6071539605962073 1102 36-37 0.6270808232562786 1102 38-39 0.5033396476295025 1102 40-41 0.6228897271647824 1102 42-43 0.6985983821701112 1102 44-45 0.6786501405999203 1102 46-47 0.5719437492086143 1102 48-49 0.5218900790924437 1102 50-51 0.7206318656255775 1102 52-53 0.5807832165882942 1102 54-55 0.4607098378489525 1102 56-57 0.5991628890766876 1102 58-59 0.48804533540017303 1102 60-61 0.4598996058781424 1102 62-63 0.3309323912870923 1102 64-65 0.40052173633555554 1102 66-67 0.32763261571168556 1102 68-69 0.373474227025163 1102 70-71 0.4277354676192999 1102 72-73 0.27334426269358403 1102 74-75 0.14853908853017117 1102 76-77 0.1599976144960884 1102 78-79 0.12808320561231668 1102 80-81 0.0978508739698718 1102 82-83 0.09222077419590491 1102 84-85 -0.0021967731046856898 1102 86-87 -0.11150860768064419 1102 88-89 -0.12901445031639014 1102 90-91 -0.3223946367740318 1102 92-93 -0.3820514248228122 1102 94-95 -0.44394370749780165 1102 96-97 -0.5817575002779805 1102 98-99 -0.6457132340676601 1102 100-101 -0.2446615010884443 1103 1 0.23572107001179532 1103 2 0.3953150916931918 1103 3 0.4843230128426619 1103 4 0.35023955777685245 1103 5 0.4234303355063318 1103 6 0.3383024156159564 1103 7 0.405357193076739 1103 8 0.4190291462952942 1103 9 0.5529426278664502 1103 10-11 0.5096066777940322 1103 12-13 0.5128141871717204 1103 14-15 0.6014522558460556 1103 16-17 0.6577624447050638 1103 18-19 0.6976638537888178 1103 20-21 0.6787062492490747 1103 22-23 0.6076605110928597 1103 24-25 0.5895390256500193 1103 26-27 0.6284111509798933 1103 28-29 0.6609610029207573 1103 30-31 0.7534080234252158 1103 32-33 0.6772143926140899 1103 34-35 0.6889139679314553 1103 36-37 0.6823641328404975 1103 38-39 0.7472880731944542 1103 40-41 0.8572129437417502 1103 42-43 0.8387420113459569 1103 44-45 0.8329875049255051 1103 46-47 0.8007083515246478 1103 48-49 0.7578067540239672 1103 50-51 0.8525574668907652 1103 52-53 0.6957741859902242 1103 54-55 0.708990322686958 1103 56-57 0.7212775691954434 1103 58-59 0.7303203383034642 1103 60-61 0.670345247244164 1103 62-63 0.6521814719094792 1103 64-65 0.6077421741952378 1103 66-67 0.45421727940526324 1103 68-69 0.43229281372526174 1103 70-71 0.4609279309692269 1103 72-73 0.5304765561518572 1103 74-75 0.40072984211323615 1103 76-77 0.360705446957585 1103 78-79 0.33043037700916855 1103 80-81 0.39927216182470815 1103 82-83 0.2627230786090031 1103 84-85 0.2343615588794279 1103 86-87 0.312958923278682 1103 88-89 0.3526936437950994 1103 90-91 0.278760105745139 1103 92-93 0.11744113383439725 1103 94-95 0.15327385249450742 1103 96-97 0.13000207756698856 1103 98-99 0.21577495254812007 1103 100-101 0.6508676394026303 1104 1 0.0833950733118165 1104 2 0.12776750827765326 1104 3 0.07747770262236742 1104 4 0.16073655943790754 1104 5 0.18851380486851355 1104 6 0.15359226056791186 1104 7 0.2960378875056193 1104 8 0.3094356209188476 1104 9 0.2974384967664747 1104 10-11 0.3031117810149482 1104 12-13 0.33821214400645516 1104 14-15 0.40718636172388045 1104 16-17 0.4119461943317475 1104 18-19 0.42541002644213677 1104 20-21 0.39014420988176823 1104 22-23 0.27976097131842437 1104 24-25 0.2012116946988698 1104 26-27 0.20096043740116443 1104 28-29 0.2643878258407284 1104 30-31 0.31839604718228287 1104 32-33 0.2589693634863437 1104 34-35 0.4047920950998929 1104 36-37 0.3956984469432072 1104 38-39 0.4167884825230601 1104 40-41 0.4522847570371269 1104 42-43 0.43433549090408974 1104 44-45 0.3807007159994029 1104 46-47 0.347760992342792 1104 48-49 0.4246835132675386 1104 50-51 0.5878882253824713 1104 52-53 0.501813770247054 1104 54-55 0.3897112489012713 1104 56-57 0.29772141168424326 1104 58-59 0.3419270400312584 1104 60-61 0.3692640456121481 1104 62-63 0.39480244741987747 1104 64-65 0.47764109238637786 1104 66-67 0.3448657492118272 1104 68-69 0.44070716633742535 1104 70-71 0.42791321887656153 1104 72-73 0.2120873511657173 1104 74-75 0.12481862363885199 1104 76-77 0.2581540711111181 1104 78-79 0.12499495956851092 1104 80-81 0.11180382751888374 1104 82-83 0.09687826631776986 1104 84-85 0.20650106127053647 1104 86-87 0.1652680969317224 1104 88-89 0.307857256552019 1104 90-91 0.04545045900428235 1104 92-93 0.0371538668522291 1104 94-95 0.1928369252578399 1104 96-97 0.06274487457036315 1104 98-99 0.19054907657661957 1104 100-101 0.5032055185008133 1105 1 0.01639775899171525 1105 2 0.07642174632479737 1105 3 0.14294565457516129 1105 4 0.1243941776977735 1105 5 0.24630639976651736 1105 6 0.19599790310052612 1105 7 0.1691987960236574 1105 8 0.17539065012067567 1105 9 0.20315195379215822 1105 10-11 0.2188828049056113 1105 12-13 0.14753292032302312 1105 14-15 0.19247817145967616 1105 16-17 0.29138327183046187 1105 18-19 0.23276725614962146 1105 20-21 0.3890355014577409 1105 22-23 0.2785107177518569 1105 24-25 0.32030291887303974 1105 26-27 0.3687438316962073 1105 28-29 0.3117669496631379 1105 30-31 0.35625679159569046 1105 32-33 0.39592145894155806 1105 34-35 0.28694285785105933 1105 36-37 0.48715722501621883 1105 38-39 0.49820939870444647 1105 40-41 0.4165210739238674 1105 42-43 0.40944078766840875 1105 44-45 0.5219452564733515 1105 46-47 0.5872966729883373 1105 48-49 0.5081935952631724 1105 50-51 0.5475812299954512 1105 52-53 0.4384251991046071 1105 54-55 0.3414046079338533 1105 56-57 0.3296955314961494 1105 58-59 0.42279932116670693 1105 60-61 0.36334651389490347 1105 62-63 0.3178551337948434 1105 64-65 0.3255942787682926 1105 66-67 0.21534387959315637 1105 68-69 0.23925927593703022 1105 70-71 0.22382057688906798 1105 72-73 0.31620431394909687 1105 74-75 0.21724405887245268 1105 76-77 0.1846796578165808 1105 78-79 0.16968722254568647 1105 80-81 0.39487002124819526 1105 82-83 0.33018876122707397 1105 84-85 0.2101443556343625 1105 86-87 0.3069720602092083 1105 88-89 0.2739139890598423 1105 90-91 0.42023041451153276 1105 92-93 0.5045039406851437 1105 94-95 0.602476092104574 1105 96-97 0.6027633473080591 1105 98-99 0.6685953743562898 1105 100-101 0.9401949521812298 1106 1 0.042983386415183844 1106 2 0.011522587819925434 1106 3 0.13004112436394522 1106 4 0.13549282917348648 1106 5 0.19092274774473594 1106 6 0.20455234023200575 1106 7 0.15957042211960015 1106 8 0.12133778980578569 1106 9 0.16919588805556174 1106 10-11 0.17242073533986968 1106 12-13 0.18301387058580332 1106 14-15 0.2298739534019134 1106 16-17 0.33295814944759883 1106 18-19 0.31818711447426296 1106 20-21 0.30953743136016243 1106 22-23 0.37409020975452023 1106 24-25 0.23225011805820373 1106 26-27 0.10753740161965197 1106 28-29 0.1726221663366232 1106 30-31 -0.004614963743236444 1106 32-33 0.14834360684380954 1106 34-35 0.20008860466086276 1106 36-37 0.299965498237313 1106 38-39 0.21375782541741728 1106 40-41 0.3059896823625152 1106 42-43 0.525385803097322 1106 44-45 0.5498918823181072 1106 46-47 0.34157686003248955 1106 48-49 0.29462961267946497 1106 50-51 0.3122506021746645 1106 52-53 0.1758929030380969 1106 54-55 0.08145642125661823 1106 56-57 0.3679661990541021 1106 58-59 0.38534289745118855 1106 60-61 0.4150490243403553 1106 62-63 0.35194513988707143 1106 64-65 0.4578343160654299 1106 66-67 0.40994388462978293 1106 68-69 0.43475253201076924 1106 70-71 0.5003010353630586 1106 72-73 0.542383351167814 1106 74-75 0.42012993529580456 1106 76-77 0.4290551414991448 1106 78-79 0.4712020338176579 1106 80-81 0.36948362648284316 1106 82-83 0.3914256981279216 1106 84-85 0.6926005177323304 1106 86-87 0.6605522539471167 1106 88-89 0.8507639480687423 1106 90-91 0.807989799176621 1106 92-93 0.9639805568389619 1106 94-95 0.9533323317613807 1106 96-97 0.9429137206382592 1106 98-99 1.1521887898927687 1106 100-101 1.5013234254477652 1107 1 -0.02758814675562249 1107 2 0.002355519230743397 1107 3 0.07696282925390818 1107 4 0.11462169495742103 1107 5 0.07762983830496495 1107 6 5.152480140466764E-4 1107 7 0.008881572004497684 1107 8 0.008817839804216021 1107 9 0.045905622439882166 1107 10-11 -0.008689788542348253 1107 12-13 0.06456615120437448 1107 14-15 0.07991995180008615 1107 16-17 0.17983809408129758 1107 18-19 0.22339427679172985 1107 20-21 0.07769909880953207 1107 22-23 0.07688714400560315 1107 24-25 -0.009695341778900968 1107 26-27 -0.03253393245553582 1107 28-29 -0.046500316999718905 1107 30-31 0.005382554193808176 1107 32-33 0.01578840714838492 1107 34-35 -0.07109874023446139 1107 36-37 -0.006049117464158371 1107 38-39 -0.028117624197676605 1107 40-41 0.06026959099464335 1107 42-43 -0.028437919704643377 1107 44-45 0.263346967007827 1107 46-47 0.39367320030960684 1107 48-49 0.2960150321513382 1107 50-51 0.22170477090810436 1107 52-53 0.20595336417640198 1107 54-55 0.2517926825528747 1107 56-57 0.37730033455014933 1107 58-59 0.4455860140940402 1107 60-61 0.4029363824224461 1107 62-63 0.34589643787788305 1107 64-65 0.30226791460508196 1107 66-67 0.006645212611111617 1107 68-69 0.1685502772675065 1107 70-71 0.256631654689528 1107 72-73 0.28690896591514914 1107 74-75 0.49401876889832863 1107 76-77 0.27123595732658146 1107 78-79 0.21148280974832545 1107 80-81 0.20451757626415912 1107 82-83 0.058492960869298116 1107 84-85 0.2932320591102524 1107 86-87 0.11648868078326302 1107 88-89 0.19588842844342835 1107 90-91 0.31116119105877615 1107 92-93 0.4312098201282559 1107 94-95 0.44418651858618574 1107 96-97 0.5186805687405354 1107 98-99 0.6394777611604958 1107 100-101 1.0174852399056675 1108 1 0.1288077784436652 1108 2 0.09441853464874228 1108 3 0.04969031003942348 1108 4 0.1633958161140896 1108 5 0.15096468747893965 1108 6 0.17389217841691362 1108 7 0.09066476461004669 1108 8 0.07266588370020344 1108 9 0.12869156559472827 1108 10-11 0.2608606055183529 1108 12-13 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-1.505547598987782 2316 96-97 -1.7801083434129943 2316 98-99 -2.09731465134654 2316 100-101 -2.0731177836814147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16966.0 3 3470.0 4 3356.0 5 3766.0 6 4769.0 7 5939.0 8 7317.0 9 8918.0 10 10834.0 11 14012.0 12 17441.0 13 21488.0 14 23064.0 15 25293.0 16 29402.0 17 35353.0 18 32150.0 19 26342.0 20 27160.0 21 29239.0 22 32533.0 23 36771.0 24 42284.0 25 48512.0 26 55138.0 27 64708.0 28 75332.0 29 89225.0 30 105354.0 31 125986.0 32 153581.0 33 190938.0 34 249260.0 35 342417.0 36 497795.0 37 710145.0 38 603583.0 39 94848.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.77200980245892 19.24896247796198 14.32430363752178 23.654724082057324 2 15.5150642083988 20.8600187962422 39.56497533317195 24.05994166218705 3 18.60721122834381 24.60820264779513 30.8321856650703 25.95240045879076 4 13.02042917280278 16.815488455296183 37.48696967010911 32.67711270179193 5 13.54562721616777 37.076851530279164 34.890686564268506 14.486834689284558 6 31.379517038369066 36.29093017117962 17.80272213606117 14.526830654390144 7 27.238880686752275 33.15028101107963 21.90649183838413 17.704346463783967 8 25.487657020658013 34.49585558327665 20.94163887207119 19.07484852399414 9 26.082020434881308 16.586159965088424 21.193389190472292 36.13843040955798 10-11 24.354885590575822 26.30452205618299 28.365250359178358 20.97534199406283 12-13 25.33247399757343 24.093788433817792 28.323851857153194 22.24988571145559 14-15 22.57226668104371 25.51063996338001 26.6737549643572 25.243338391219083 16-17 21.726165684855665 28.462567271634548 27.59402718450028 22.217239859009503 18-19 21.64150062316967 27.210384959481328 29.735625892268615 21.41248852508039 20-21 22.396288201823257 26.48887470399587 29.664504665998702 21.450332428182172 22-23 22.14965589719054 26.35176576496422 29.542714128985992 21.955864208859243 24-25 22.02766778121398 26.503082727905557 29.682953131954033 21.786296358926425 26-27 21.735708770120514 26.67534399018751 29.55414083851714 22.03480640117484 28-29 21.79668415242277 27.04134673114826 29.44427326994714 21.717695846481828 30-31 21.989500467053915 26.708399405772994 29.215906069676738 22.086194057496357 32-33 21.704049813546597 26.763037220164343 29.167306152754907 22.365606813534153 34-35 22.42099131845214 26.838099818453827 28.898925447133085 21.84198341596095 36-37 22.44364643173192 26.39441983975003 29.11758085334077 22.044352875177285 38-39 21.986655306054097 26.771683504346395 29.012313693680845 22.22934749591866 40-41 22.317774130520547 26.66996486265904 29.03422875762946 21.978032249190953 42-43 21.98990627253064 26.74954653025521 28.755981333460767 22.504565863753378 44-45 22.07792949913597 26.817736359818152 28.845792704197205 22.25854143684868 46-47 22.310385761301266 27.07891859036467 28.298335753378755 22.312359894955307 48-49 22.112157545368337 27.066418986503166 28.262935009937518 22.558488458190983 50-51 22.091819477444552 27.28179618498257 28.232809794160723 22.39357454341215 52-53 22.355959137579827 27.096020140768196 28.06073924475237 22.48728147689961 54-55 22.075533381070272 27.193300445673213 28.09964854511654 22.631517628139978 56-57 21.928718037820254 27.34466367007231 28.073437598610628 22.653180693496807 58-59 22.06053568776439 26.948551520143077 28.10409498180897 22.88681781028357 60-61 21.814046002204226 27.008125426011226 28.471579438083083 22.706249133701466 62-63 21.83208450475947 26.93839856956141 28.55483676917035 22.674680156508767 64-65 21.695632336579628 27.058152713621812 28.261937685874738 22.984277263923815 66-67 21.592101710831734 27.057520646468387 28.137687642312713 23.212690000387166 68-69 21.817016492523557 26.772491213372483 28.29659803454469 23.11389425955927 70-71 21.81041369922566 26.64644335182994 28.041257017430915 23.501885931513485 72-73 21.788593783432557 26.630618992207626 28.056360336783893 23.52442688757592 74-75 21.5713527235342 27.093638039335456 27.89721186889082 23.437797368239526 76-77 21.66351838591198 26.68817706815938 28.01357230912268 23.63473223680596 78-79 21.692341394692797 26.794684720330487 27.985572582296626 23.527401302680094 80-81 21.80655500534411 26.608952905622935 27.84579880442645 23.738693284606505 82-83 21.70249536257868 26.525049868027338 28.043690080762957 23.728764688631024 84-85 21.61977201917487 26.50135519799087 27.97777288263627 23.901099900197995 86-87 21.628523017755 26.378614516276087 27.972961635040257 24.019900830928655 88-89 21.67040634682714 26.22361577173036 27.837391129846672 24.26858675159583 90-91 21.48171301826691 26.109753719489053 27.940407108358308 24.468126153885727 92-93 21.639524437749554 25.920494087950306 27.961322080204848 24.47865939409529 94-95 21.532621150007646 25.701392045864253 28.07454681496897 24.691439989159132 96-97 21.57194155152517 25.350023815209568 28.147226067273483 24.93080856599178 98-99 21.517151007677974 25.47835487627377 27.90722510583084 25.09726901021742 100-101 21.797599425379175 24.80066865913581 28.233976144922906 25.16775577056211 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3941.0 1 2698.5 2 1454.5 3 1445.5 4 1385.0 5 1485.5 6 2072.5 7 3246.5 8 4642.0 9 5214.5 10 4491.5 11 3881.5 12 4003.0 13 4242.0 14 4553.0 15 4867.0 16 5225.0 17 5561.0 18 6003.5 19 6656.0 20 7374.5 21 8364.5 22 9965.5 23 12160.0 24 14448.5 25 17942.0 26 23462.0 27 30100.5 28 36163.0 29 41987.0 30 48131.5 31 56544.0 32 66900.0 33 72556.5 34 75248.5 35 80766.0 36 89480.0 37 97541.0 38 102288.5 39 105963.0 40 110126.0 41 114911.0 42 118195.5 43 122246.5 44 127696.5 45 132951.5 46 135829.5 47 138267.5 48 143068.0 49 142902.0 50 139748.5 51 138014.0 52 136078.5 53 135583.5 54 133368.5 55 124791.5 56 113518.0 57 102083.0 58 93779.0 59 84601.5 60 69708.0 61 56343.0 62 46435.5 63 35379.0 64 25336.5 65 18696.5 66 13574.0 67 10235.5 68 7794.5 69 5879.5 70 4383.0 71 2970.5 72 2133.5 73 1627.0 74 1154.0 75 822.0 76 576.5 77 403.5 78 276.5 79 174.0 80 111.5 81 84.5 82 66.5 83 57.0 84 44.5 85 40.5 86 40.5 87 26.0 88 16.0 89 16.5 90 12.5 91 6.0 92 5.5 93 6.0 94 3.5 95 5.0 96 6.0 97 3.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2733718011747354 2 0.16772323854374208 3 0.19675524620281007 4 0.16544621833518772 5 0.23875074391285223 6 0.19618599115067145 7 0.24157114394390253 8 0.24928196237741612 9 0.15028333376458716 10-11 0.206510207778094 12-13 0.21303076564804513 14-15 0.1733122881465573 16-17 0.2873961756410588 18-19 0.23309700623593033 20-21 0.1992780810929697 22-23 0.24657800087975779 24-25 0.23349807229539163 26-27 0.34367480011385104 28-29 0.31647993375941214 30-31 0.2921442802804875 32-33 0.19240820762284264 34-35 0.3593810645069475 36-37 0.39528294563614247 38-39 0.37662690506378244 40-41 0.338952570704065 42-43 0.3722928040986364 44-45 0.3812197583253551 46-47 0.3857996739721065 48-49 0.40494734390767717 50-51 0.3981162832820141 52-53 0.39829740988951273 54-55 0.3915051621083137 56-57 0.37076616554971925 58-59 0.3454860661888374 60-61 0.3131420291355086 62-63 0.38015887391000597 64-65 0.41415892566046525 66-67 0.41972210003363775 68-69 0.4238491991616426 70-71 0.42271068905736536 72-73 0.4140166118974306 74-75 0.40964369808782053 76-77 0.39445493828757733 78-79 0.36881258571169817 80-81 0.37946024270965406 82-83 0.38913757859601 84-85 0.3901467125520739 86-87 0.38582554920174916 88-89 0.3937563070872254 90-91 0.41450824126064123 92-93 0.4138096100602893 94-95 0.4217533055605869 96-97 0.4222578725386188 98-99 0.41978678810774445 100-101 0.39343286671669214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3864700.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.92810921154231 #Duplication Level Percentage of deduplicated Percentage of total 1 74.09478231795576 36.253176012583936 2 13.45463262762042 13.16619469210785 3 4.880310797100339 7.163531390003837 4 2.338454642291207 4.576646564970492 5 1.33259224456673 3.2600609438307635 6 0.8134200891212645 2.3879464173232625 7 0.5794952399169035 1.9847524470356221 8 0.39596179322310393 1.549892948993453 9 0.28832623869212 1.269653192575314 >10 1.6253813141613036 14.580922379084404 >50 0.12346741918934087 4.144869564419719 >100 0.0666097419177234 5.965512303646412 >500 0.00460646356630662 1.5819475072177618 >1k 0.0018002271090663993 1.3694115048497693 >5k 1.058957122980234E-4 0.41750729875442427 >10k+ 5.29478561490117E-5 0.327974832603066 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12660 0.3275804072761146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9680 0.2504722229409786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6436 0.16653297798017958 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.587522964266308E-5 5 0.0 0.0 0.0 0.0 2.587522964266308E-5 6 0.0 0.0 0.0 0.0 1.0350091857065232E-4 7 0.0 0.0 0.0 0.0 1.0350091857065232E-4 8 0.0 0.0 0.0 0.0 1.0350091857065232E-4 9 0.0 0.0 0.0 0.0 1.293761482133154E-4 10-11 0.0 0.0 0.0 1.293761482133154E-5 1.293761482133154E-4 12-13 0.0 0.0 0.0 2.587522964266308E-5 1.8112660749864155E-4 14-15 0.0 0.0 0.0 2.587522964266308E-5 2.0700183714130463E-4 16-17 0.0 0.0 0.0 9.056330374932077E-5 2.3287706678396772E-4 18-19 0.0 0.0 0.0 1.1643853339198386E-4 3.3637798535462E-4 20-21 0.0 0.0 0.0 1.6818899267731003E-4 3.493156001759516E-4 22-23 0.0 0.0 0.0 4.0106605946127775E-4 3.751908298186146E-4 24-25 0.0 0.0 0.0 0.0010479468005278546 4.3987890392527235E-4 26-27 0.0 0.0 0.0 0.0023934587419463344 4.3987890392527235E-4 28-29 0.0 0.0 0.0 0.007684943203870934 4.3987890392527235E-4 30-31 0.0 0.0 0.0 0.02480140761249256 4.3987890392527235E-4 32-33 0.0 0.0 0.0 0.06554195668486558 4.3987890392527235E-4 34-35 0.0 0.0 0.0 0.13047584547312857 4.6575413356793544E-4 36-37 0.0 0.0 0.0 0.23710766683054313 4.6575413356793544E-4 38-39 0.0 0.0 0.0 0.39097471990063914 4.916293632105985E-4 40-41 0.0 0.0 0.0 0.5566926281470748 4.916293632105985E-4 42-43 0.0 0.0 0.0 0.7395528760317748 5.045669780319301E-4 44-45 0.0 0.0 0.0 0.9658964473309701 5.563174373172562E-4 46-47 0.0 0.0 0.0 1.232191373198437 6.598183558879085E-4 48-49 0.0 0.0 0.0 1.505860739514063 7.503816596372293E-4 50-51 0.0 0.0 0.0 1.7972158252904493 7.503816596372293E-4 52-53 0.0 0.0 0.0 2.093810645069475 8.797578078505446E-4 54-55 0.0 0.0 0.0 2.431521204750692 9.573834967785339E-4 56-57 0.0 0.0 0.0 2.821072787020985 9.573834967785339E-4 58-59 0.0 0.0 0.0 3.232256061272544 9.573834967785339E-4 60-61 0.0 0.0 0.0 3.672238466116387 9.703211115998654E-4 62-63 0.0 0.0 0.0 4.116619659999483 0.0010350091857065232 64-65 0.0 0.0 0.0 4.597601366212125 0.0010350091857065232 66-67 0.0 0.0 0.0 5.140670685952339 0.0010479468005278546 68-69 0.0 0.0 0.0 5.741584081558724 0.0010608844153491863 70-71 0.0 0.0 0.0 6.37291380961006 0.0010608844153491863 72-73 0.0 0.0 0.0 7.015861515770952 0.00117732294874117 74-75 0.0 0.0 0.0 7.680867855202215 0.001229073408026496 76-77 0.0 0.0 0.0 8.391854477708488 0.001267886252490491 78-79 0.0 0.0 0.0 9.183338939633089 0.001267886252490491 80-81 0.0 0.0 0.0 10.012885864362048 0.0012808238673118226 82-83 0.0 0.0 0.0 10.865164178332083 0.001319636711775817 84-85 0.0 0.0 0.0 11.738323802623748 0.001319636711775817 86-87 0.0 0.0 0.0 12.64496597407302 0.0013325743265971486 88-89 0.0 0.0 0.0 13.592516883587342 0.00134551194141848 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10960 0.0 33.824856 1 GTATCAA 16680 0.0 32.48334 1 TATCAAC 20365 0.0 26.347908 2 ATCAACG 20275 0.0 26.347868 3 CAACGCA 20640 0.0 25.881927 5 TCAACGC 20730 0.0 25.815334 4 AACGCAG 21215 0.0 25.110418 6 TACATGG 11705 0.0 22.94103 2 GTACATG 12070 0.0 22.720634 1 ACGCAGA 23485 0.0 22.584072 7 CGCAGAG 23450 0.0 22.435707 8 GAGTACT 12980 0.0 21.957973 12-13 ACATGGG 12170 0.0 20.856007 3 GCAGAGT 24730 0.0 20.640865 9 AGTACTT 13685 0.0 20.341227 12-13 AGAGTAC 19435 0.0 20.158503 10-11 GTACTTT 14050 0.0 19.880869 14-15 CATGGGG 7250 0.0 17.930008 4 CAGAGTA 23830 0.0 17.755096 10-11 TACTTTT 16915 0.0 16.092615 14-15 >>END_MODULE