FastQCFastQC Report
Thu 26 May 2016
SRR938782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864700
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121540.31448754107692706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99590.2576914120112816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.17621031386653555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111800.032.7626531
GTATCAA166000.031.5507281
ATCAACG204800.025.4674243
TATCAAC205600.025.4375782
CAACGCA208000.025.0299825
TCAACGC209500.024.8281174
AACGCAG213750.024.393816
GAGTACT136550.021.68220312-13
TACATGG113550.021.566512
ACGCAGA241150.021.5625487
GTACATG117500.021.537931
CGCAGAG240650.021.528498
ACATGGG117900.019.885223
AGTACTT142800.019.86934312-13
GTACTTT145750.019.7438914-15
GCAGAGT258050.019.709149
AGAGTAC202500.019.68179910-11
CATGGGG69050.018.007564
CAGAGTA246650.016.96672210-11
TATTCCG2858.673487E-716.652235
Produced by FastQC (version 0.11.5)