##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938782_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3864700 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04455895671074 33.0 31.0 34.0 30.0 34.0 2 32.24828110849484 34.0 31.0 34.0 30.0 34.0 3 32.331814112350244 34.0 31.0 34.0 30.0 34.0 4 35.963011876730405 37.0 35.0 37.0 35.0 37.0 5 35.86126322871115 37.0 35.0 37.0 35.0 37.0 6 35.877017621031385 37.0 35.0 37.0 35.0 37.0 7 35.791594690402874 37.0 35.0 37.0 33.0 37.0 8 35.7771110306104 37.0 35.0 37.0 33.0 37.0 9 37.42373069060988 39.0 37.0 39.0 34.0 39.0 10-11 37.357844463994624 39.0 37.0 39.0 34.0 39.0 12-13 37.28154086992522 39.0 37.0 39.0 33.0 39.0 14-15 38.50896007452066 40.0 38.0 41.0 34.0 41.0 16-17 38.44421015861516 40.0 38.0 41.0 33.0 41.0 18-19 38.564446658214095 40.0 38.0 41.0 34.0 41.0 20-21 38.55573175149429 40.0 38.0 41.0 34.0 41.0 22-23 38.478200119026056 40.0 38.0 41.0 34.0 41.0 24-25 38.39848578156131 40.0 38.0 41.0 34.0 41.0 26-27 38.221877377286724 40.0 38.0 41.0 33.0 41.0 28-29 38.04740018630165 40.0 37.5 41.0 33.0 41.0 30-31 37.82217326053769 40.0 37.0 41.0 33.0 41.0 32-33 37.66680001035009 40.0 37.0 41.0 32.5 41.0 34-35 37.634185059642405 40.0 37.0 41.0 32.0 41.0 36-37 37.51258402980827 40.0 37.0 41.0 32.0 41.0 38-39 37.3852962713794 40.0 36.5 41.0 31.5 41.0 40-41 37.20967345460191 40.0 36.0 41.0 31.0 41.0 42-43 37.01049292312469 40.0 36.0 41.0 31.0 41.0 44-45 36.77085698760577 39.5 36.0 41.0 30.5 41.0 46-47 36.47332432012834 39.0 35.0 41.0 30.0 41.0 48-49 36.176235154086996 39.0 35.0 41.0 29.5 41.0 50-51 35.943218619815255 39.0 35.0 41.0 29.0 41.0 52-53 35.71628224700494 39.0 35.0 40.0 28.0 41.0 54-55 35.43712345072062 38.5 35.0 40.0 28.0 41.0 56-57 35.15608391336973 38.0 34.0 40.0 27.0 41.0 58-59 34.84294667115171 38.0 34.0 40.0 26.5 41.0 60-61 34.482689988873645 37.5 34.0 40.0 26.0 41.0 62-63 34.108930576758866 37.0 33.5 40.0 26.0 41.0 64-65 33.70715488912464 36.0 33.0 39.0 25.0 41.0 66-67 33.30449142236138 36.0 33.0 39.0 24.0 40.0 68-69 32.97276722643414 35.5 32.0 39.0 23.0 40.0 70-71 32.55570238310865 35.0 32.0 38.0 22.0 40.0 72-73 32.09027091365436 35.0 31.5 37.0 21.5 39.5 74-75 31.61382772272104 35.0 31.0 37.0 20.0 39.0 76-77 29.648883354464772 33.0 28.5 35.0 17.5 37.0 78-79 30.61076849432039 34.0 30.5 36.0 18.0 38.0 80-81 30.685122001707768 34.0 31.0 35.5 18.0 37.0 82-83 30.385612337309496 34.0 30.5 35.0 15.5 37.0 84-85 30.013280332237947 34.0 30.0 35.0 9.0 36.0 86-87 29.46417238077988 34.0 29.5 35.0 4.5 36.0 88-89 28.88831215877041 34.0 29.0 35.0 2.0 35.5 90-91 28.39557857013481 34.0 29.0 35.0 2.0 35.0 92-93 28.121635961394155 33.0 28.5 35.0 2.0 35.0 94-95 27.810634201878543 33.0 27.5 35.0 2.0 35.0 96-97 27.25030493958134 33.0 27.0 35.0 2.0 35.0 98-99 26.467730483608044 33.0 25.0 35.0 2.0 35.0 100-101 22.99292040779362 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07403880228047655 1101 2 0.10506507029553802 1101 3 0.016606458785311418 1101 4 0.08133191781668359 1101 5 -0.04358440082791759 1101 6 0.05336613970708726 1101 7 0.1039836815126094 1101 8 0.14362945798899318 1101 9 0.1238580510362084 1101 10-11 0.14153910130048786 1101 12-13 0.09961580678564985 1101 14-15 0.22731798466577402 1101 16-17 0.22105888114397487 1101 18-19 0.20462861010318534 1101 20-21 0.17867121281300768 1101 22-23 0.029916605842736033 1101 24-25 0.1286559151830815 1101 26-27 0.06636400574195278 1101 28-29 0.017801900197845555 1101 30-31 0.005427552503114441 1101 32-33 0.10693134876222388 1101 34-35 0.1890233747178982 1101 36-37 0.26729272071492005 1101 38-39 0.2520668183367718 1101 40-41 0.26898750016859907 1101 42-43 0.29761798205588974 1101 44-45 0.3315723442601808 1101 46-47 0.27918735937130634 1101 48-49 0.23133077372671806 1101 50-51 0.15746986866234636 1101 52-53 0.0827441292199822 1101 54-55 0.0739474252429062 1101 56-57 0.11204519414361158 1101 58-59 0.07207904993638437 1101 60-61 0.1091371716655658 1101 62-63 -0.021746709222213667 1101 64-65 0.09025933113331774 1101 66-67 -0.06422964730064251 1101 68-69 0.016172305670572484 1101 70-71 0.15177435397921357 1101 72-73 0.08928314167793872 1101 74-75 -0.024960791615743716 1101 76-77 -0.09213103163963865 1101 78-79 -0.20376005067342007 1101 80-81 -0.004066149143074682 1101 82-83 2.8184893600879946E-4 1101 84-85 0.10696046168427031 1101 86-87 0.11244751975675271 1101 88-89 0.10085763035490203 1101 90-91 -0.023697092113245333 1101 92-93 -0.1066412501987557 1101 94-95 0.11193627145006602 1101 96-97 0.030146088253047765 1101 98-99 -0.17380152281103634 1101 100-101 0.4045538840007552 1102 1 0.27448311636943856 1102 2 0.199603232868931 1102 3 0.19880782412981546 1102 4 0.14495271657997222 1102 5 0.05601989561998266 1102 6 0.057489277978078235 1102 7 0.14576936216349168 1102 8 0.17468500148522992 1102 9 0.24501888099293012 1102 10-11 0.28599526768921635 1102 12-13 0.24613390121814405 1102 14-15 0.34401697461174763 1102 16-17 0.3000896938501256 1102 18-19 0.30905760282854544 1102 20-21 0.27629002867984553 1102 22-23 0.2541837438564656 1102 24-25 0.2839632067061828 1102 26-27 0.29632213693813725 1102 28-29 0.31270818063326544 1102 30-31 0.29270498630319963 1102 32-33 0.3739389711126151 1102 34-35 0.4761793539574981 1102 36-37 0.4030396188860337 1102 38-39 0.39216835061669286 1102 40-41 0.5376131528074097 1102 42-43 0.47985455630224294 1102 44-45 0.49310777109112536 1102 46-47 0.5758271308874257 1102 48-49 0.5089602864958067 1102 50-51 0.5738849406277637 1102 52-53 0.37084945673957037 1102 54-55 0.3942305306551219 1102 56-57 0.34535369820243744 1102 58-59 0.468160388888208 1102 60-61 0.4813628465853981 1102 62-63 0.3081179550808244 1102 64-65 0.2907890653918912 1102 66-67 0.35127918126868707 1102 68-69 0.3818436335508011 1102 70-71 0.425062332791569 1102 72-73 0.42871622882891813 1102 74-75 0.35033713814389955 1102 76-77 0.2741456309383459 1102 78-79 0.20171406141621873 1102 80-81 0.4133892543502249 1102 82-83 0.35170405169104413 1102 84-85 0.49935645867977385 1102 86-87 0.5629294833238276 1102 88-89 0.549628606390165 1102 90-91 0.5230531872609561 1102 92-93 0.5072883317104306 1102 94-95 0.6030068130053294 1102 96-97 0.6544088906548069 1102 98-99 0.90038352570188 1102 100-101 1.4141553981916992 1103 1 0.2300862549261069 1103 2 0.19036693156002826 1103 3 0.1562561461367551 1103 4 0.10300077089014792 1103 5 -0.050484437852091446 1103 6 0.07023270642373092 1103 7 0.09274101778350285 1103 8 0.12261509855285624 1103 9 0.17924689624774715 1103 10-11 0.20907550268820785 1103 12-13 0.16037444233067788 1103 14-15 0.27009647156561556 1103 16-17 0.12069909533565237 1103 18-19 0.07003817856590189 1103 20-21 0.19111401961082208 1103 22-23 0.2853673865499431 1103 24-25 0.29617457582227047 1103 26-27 0.3228655187049796 1103 28-29 0.22818389479505896 1103 30-31 0.3354920362673326 1103 32-33 0.3425686273028319 1103 34-35 0.2709607787293038 1103 36-37 0.37258063506566685 1103 38-39 0.29175310133856414 1103 40-41 0.4161218693407207 1103 42-43 0.3921077512718938 1103 44-45 0.33324414555465154 1103 46-47 0.16707011387819648 1103 48-49 0.25580257936352524 1103 50-51 0.2929763341795848 1103 52-53 0.18808746555486522 1103 54-55 0.14647380496462858 1103 56-57 0.10020188751948211 1103 58-59 0.2114959078184171 1103 60-61 0.21162713285875157 1103 62-63 0.17678240845979332 1103 64-65 0.20971970384330518 1103 66-67 0.2580731875624167 1103 68-69 0.2106403346134158 1103 70-71 0.10320069873366577 1103 72-73 0.1908469272394342 1103 74-75 0.344569429935067 1103 76-77 0.2148787637675369 1103 78-79 -0.012222530561523115 1103 80-81 0.2481427311728659 1103 82-83 0.3329044572202804 1103 84-85 0.3861015694840262 1103 86-87 0.36327213603093256 1103 88-89 0.25166556289777375 1103 90-91 0.2064856918758622 1103 92-93 0.30007940814585154 1103 94-95 0.4322894808971718 1103 96-97 0.48109077597016636 1103 98-99 0.47235881927401735 1103 100-101 0.982409773129362 1104 1 0.13672069053013303 1104 2 0.12167369726784472 1104 3 0.07850399778260453 1104 4 0.036804496304895906 1104 5 -0.045508321334480684 1104 6 0.032242981869032405 1104 7 0.043108475082775044 1104 8 -0.007570443508072344 1104 9 0.04113409709698601 1104 10-11 0.11558538702183085 1104 12-13 0.01172150054697596 1104 14-15 0.021313554838457094 1104 16-17 0.1658687693577292 1104 18-19 0.1174875203555672 1104 20-21 0.16000235432515098 1104 22-23 0.04990552145780214 1104 24-25 0.10464259933645081 1104 26-27 0.13452764402301653 1104 28-29 0.02111143893933587 1104 30-31 -0.11924527302776511 1104 32-33 -0.030856713422515725 1104 34-35 0.05384562628406542 1104 36-37 0.07825915174491627 1104 38-39 0.034799621272561865 1104 40-41 0.08585288827913473 1104 42-43 0.06151700701146012 1104 44-45 0.009039741808152257 1104 46-47 -0.07233447170625595 1104 48-49 0.10231808334005876 1104 50-51 0.04063380424725693 1104 52-53 -0.09773410084607548 1104 54-55 -0.1264948983717744 1104 56-57 -0.17208409544603853 1104 58-59 0.10643377157023082 1104 60-61 0.21226044674230593 1104 62-63 0.1375051480853955 1104 64-65 0.01947843733698562 1104 66-67 0.025955230816002484 1104 68-69 0.10718108006070537 1104 70-71 -0.019094020071655393 1104 72-73 0.08393791301202214 1104 74-75 0.0749560895210557 1104 76-77 0.0610458002008194 1104 78-79 -0.07638568679146118 1104 80-81 0.013733376068291392 1104 82-83 0.0328162155778422 1104 84-85 0.0328221379843292 1104 86-87 0.24815964380762168 1104 88-89 0.30342217053243203 1104 90-91 0.2530356423865676 1104 92-93 0.20194170150115198 1104 94-95 0.15956077164303295 1104 96-97 0.14287052460983318 1104 98-99 0.5586780249281098 1104 100-101 1.1764713479652826 1105 1 0.08174597430537034 1105 2 0.005829913437878531 1105 3 0.014337856244480918 1105 4 0.014728177863347014 1105 5 -0.035002196394422924 1105 6 -0.071147731177021 1105 7 -0.02266510640545505 1105 8 0.03526115036648747 1105 9 0.05065510833462383 1105 10-11 0.05843634170754086 1105 12-13 0.05598875694120409 1105 14-15 0.057884770704141886 1105 16-17 0.08133119447039405 1105 18-19 0.018028961736575866 1105 20-21 -0.03473744352611874 1105 22-23 -0.014596616869212653 1105 24-25 -0.06178719150605616 1105 26-27 0.03904760050471623 1105 28-29 0.024635052583114714 1105 30-31 0.006142567411075106 1105 32-33 0.01763246847962563 1105 34-35 0.0550887266753719 1105 36-37 -0.008661510228819225 1105 38-39 0.0416205112976229 1105 40-41 -0.06851067684721102 1105 42-43 -0.0672371109568175 1105 44-45 -0.04516961840510447 1105 46-47 -0.19142411003497273 1105 48-49 -0.08888956429506578 1105 50-51 -0.1424009020143444 1105 52-53 -0.07827702549871418 1105 54-55 -0.1309934695587529 1105 56-57 -0.09726073501116872 1105 58-59 -0.10134953769649968 1105 60-61 -0.12142475308164791 1105 62-63 -0.10314194029558621 1105 64-65 -0.11953171436836385 1105 66-67 -0.18873136495684406 1105 68-69 -0.07955259386274349 1105 70-71 -0.09220574713563678 1105 72-73 -0.163669079932113 1105 74-75 -0.1860710700056849 1105 76-77 -0.01366574125851372 1105 78-79 -0.13123161212013912 1105 80-81 -0.12353237937382389 1105 82-83 -0.005348133887434159 1105 84-85 -0.02296159561415223 1105 86-87 0.1493112982763236 1105 88-89 0.08927864247572259 1105 90-91 0.04475495124947315 1105 92-93 0.11444723340249041 1105 94-95 0.28566362593541683 1105 96-97 0.44467243645113186 1105 98-99 0.6936513226647243 1105 100-101 1.3135505135055503 1106 1 0.14861555006348226 1106 2 0.02023349262690033 1106 3 0.03419058318008439 1106 4 0.026662178736799547 1106 5 -0.04218806711568135 1106 6 0.012301807756301741 1106 7 0.04571023818269282 1106 8 0.02216605036529984 1106 9 0.024515666051044605 1106 10-11 0.027933138295260562 1106 12-13 0.07566249441472195 1106 14-15 0.09521531136257977 1106 16-17 0.16168141359629118 1106 18-19 -0.10958409847238215 1106 20-21 -0.034330892466137186 1106 22-23 0.0691793568275898 1106 24-25 0.09176051859664369 1106 26-27 0.02714328441539493 1106 28-29 0.12657119858997845 1106 30-31 0.12000080775709421 1106 32-33 0.058272732085718815 1106 34-35 0.0756857914178255 1106 36-37 -0.007278427632968487 1106 38-39 0.09308143326916962 1106 40-41 0.2596207840786704 1106 42-43 0.18768188611763037 1106 44-45 0.14974119610753434 1106 46-47 0.1486788981231939 1106 48-49 0.053376590392801404 1106 50-51 0.03769198591587042 1106 52-53 0.074083782548513 1106 54-55 0.11024674636901466 1106 56-57 0.007036329953102438 1106 58-59 0.08494459949992716 1106 60-61 0.13925853976076752 1106 62-63 0.1898838056112666 1106 64-65 0.14146460615370415 1106 66-67 0.10247597588801938 1106 68-69 0.14358277436362954 1106 70-71 0.19295203042785403 1106 72-73 0.2220412064914754 1106 74-75 0.2781570362264354 1106 76-77 0.12827547921540017 1106 78-79 0.07755028222316795 1106 80-81 0.061715418113056586 1106 82-83 0.12150290812678222 1106 84-85 0.12233165677997349 1106 86-87 0.19112114237705669 1106 88-89 0.3838263044303538 1106 90-91 0.22231598805873531 1106 92-93 0.40911275373630573 1106 94-95 0.5121859954101176 1106 96-97 0.6921004619406403 1106 98-99 0.6918963647512228 1106 100-101 1.2573687322384366 1107 1 -0.026179139460595025 1107 2 -0.03368835448345919 1107 3 -0.03703511204665233 1107 4 -0.029141726901841025 1107 5 -0.10956009431795621 1107 6 -0.11745385656887919 1107 7 0.045999208285742554 1107 8 -0.007305276695923624 1107 9 -0.026441811085959444 1107 10-11 -0.10921827776823534 1107 12-13 -0.013328229942615621 1107 14-15 -0.03653176202250563 1107 16-17 0.011083699708265726 1107 18-19 -0.023872693030767778 1107 20-21 -0.07587209179947507 1107 22-23 -0.08083731104042613 1107 24-25 -0.01230650590292015 1107 26-27 -0.1344537018063079 1107 28-29 -0.05780761209810947 1107 30-31 -0.03312746507519648 1107 32-33 -0.12734989237103633 1107 34-35 -5.497110633925217E-4 1107 36-37 -0.08142012625177131 1107 38-39 -0.07678889123531718 1107 40-41 -0.28501147655398995 1107 42-43 -0.19883985852674613 1107 44-45 -0.06356707417022989 1107 46-47 -0.1741164301632807 1107 48-49 -0.17103292644220147 1107 50-51 -0.28088584118683 1107 52-53 -0.3588867679945267 1107 54-55 -0.20072985999352966 1107 56-57 -0.2896480972241662 1107 58-59 -0.1930476807407686 1107 60-61 -0.19130721141979734 1107 62-63 -0.1652764330342933 1107 64-65 -0.2069228876136293 1107 66-67 -0.22701762244715695 1107 68-69 -0.3481698014884387 1107 70-71 -0.2564407376585578 1107 72-73 -0.22884083171961223 1107 74-75 -0.20293441278198543 1107 76-77 -0.2368222639837363 1107 78-79 -0.14766218456565383 1107 80-81 -0.057414286240632606 1107 82-83 -0.12350323426728238 1107 84-85 -0.2157150942604389 1107 86-87 -0.08797011070626937 1107 88-89 -0.14148611133252942 1107 90-91 -0.07721061472565083 1107 92-93 -0.006867927065865587 1107 94-95 0.0360551459169578 1107 96-97 0.13614812427861978 1107 98-99 0.40406800419262723 1107 100-101 1.1208713488219573 1108 1 0.14695277176480204 1108 2 0.010484536093088082 1108 3 0.034178032601602126 1108 4 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-1.5784194745360658 2316 96-97 -1.814203521003165 2316 98-99 -1.8212550364642617 2316 100-101 -1.9474005951088955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 15.0 4 33.0 5 105.0 6 244.0 7 653.0 8 1623.0 9 2823.0 10 4827.0 11 7189.0 12 10044.0 13 13456.0 14 16013.0 15 20373.0 16 25102.0 17 32697.0 18 31275.0 19 23026.0 20 22425.0 21 23229.0 22 25387.0 23 28215.0 24 33026.0 25 38846.0 26 46740.0 27 56813.0 28 69297.0 29 84614.0 30 102245.0 31 124088.0 32 152981.0 33 191279.0 34 251757.0 35 352019.0 36 532569.0 37 817889.0 38 660609.0 39 61172.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.57868883156547 20.057364332239025 14.903107238985545 23.460839597209954 2 15.8592905012032 21.472818071260384 38.77348823970813 23.894403187828292 3 18.60240122131084 24.78575309855875 31.064946826403084 25.546898853727328 4 13.309415996066964 17.45374802701374 36.999560121096074 32.23727585582322 5 13.401234952941909 37.451421966239515 34.877805296523036 14.269537784295542 6 31.450226027734 36.748564523349295 17.407033641517685 14.394175807399012 7 27.222087096022978 33.33663673765105 21.655523067767227 17.78575309855875 8 25.541465055502364 35.017207027712374 20.622687401350685 18.818640515434574 9 25.90001811266075 16.66147437058504 20.977022796077314 36.46148472067689 10-11 24.45938882707584 26.598675188242293 28.112880689316118 20.829055295365748 12-13 25.3451626258183 24.20830853623826 28.23184205759826 22.214686780345176 14-15 22.434186353403888 25.70821797293451 26.514076124925605 25.343519548735994 16-17 21.65388412690999 28.740770507750966 27.4855375672631 22.119807798075936 18-19 21.60394079747458 27.354076642430204 29.689471369058403 21.35251119103682 20-21 22.320969286102414 26.57413253292623 29.672975910161203 21.43192227081015 22-23 22.139454550159133 26.46901441250291 29.5095220845085 21.88200895282946 24-25 22.056723825196848 26.664726019421998 29.566938098527956 21.711612056853202 26-27 21.780541521995104 26.786034265247427 29.529236130257257 21.904188082500216 28-29 21.80345175563433 27.14728180712604 29.45075943799001 21.59850699924962 30-31 22.016948275415945 26.75942505239734 29.204181437110254 22.01944523507646 32-33 21.78409708386162 26.793916733511008 29.145496416280693 22.27648976634668 34-35 22.40449969698052 26.92677434597217 28.955529885587506 21.71319607145981 36-37 22.509428310228124 26.484387080578493 29.118102808533326 21.88808180066006 38-39 22.01839705777505 26.837580472085648 29.00939029160484 22.134632178534456 40-41 22.325622919692446 26.739882710278863 29.023014792219932 21.91147957780876 42-43 22.095197110319234 26.73416621564464 28.772233725458328 22.398402948577793 44-45 22.126931405102386 26.85866689575795 28.859278093353318 22.155123605786347 46-47 22.404565861968944 27.10510906333537 28.249226779648822 22.241098295046864 48-49 22.157843434825665 27.131881032762024 28.203459221290956 22.50681631112136 50-51 22.185889063762428 27.34273510410582 28.12081670040152 22.350559131730233 52-53 22.433423034129426 27.18265324604756 27.976906357543925 22.407017362279092 54-55 22.13368437394882 27.244520920123165 27.975043341009652 22.646751364918362 56-57 21.968164233369826 27.422142306917035 27.871523582878332 22.738169876834803 58-59 22.051987120305704 27.101113983845526 27.953925388026146 22.892973507822624 60-61 21.801661232727838 27.253221549448842 28.232197381359004 22.71291983646432 62-63 21.79923745007653 27.1583694835128 28.288984944361285 22.753408122049386 64-65 21.616325155506196 27.300002561689496 28.01061263968647 23.07305964311784 66-67 21.573349200970533 27.324384368928257 27.84624124993094 23.256025180170266 68-69 21.80217724983 27.008659422277375 27.99976039487752 23.189402933015103 70-71 21.750734921780413 26.94895615361308 27.754840806407334 23.545468118199175 72-73 21.72670582451419 26.987062385178668 27.713819960152147 23.572411830154994 74-75 21.5732987121193 27.37878208623865 27.461337380237577 23.586581821404476 76-77 21.628880432735283 27.020906002279666 27.732288734422767 23.617924830562284 78-79 21.708477322045113 27.133841127153545 27.63790364614205 23.519777904659296 80-81 21.760177298564496 27.02576612347634 27.462026163958477 23.75203041400069 82-83 21.672574249957727 26.948852365101196 27.61844459759842 23.76012878734266 84-85 21.55803814008849 26.922568375294333 27.545618029860012 23.97377545475716 86-87 21.58673584363174 26.825621724951628 27.527087547887263 24.060554883529374 88-89 21.540023891294997 26.744345616495252 27.349261988523814 24.366368503685933 90-91 21.32347994363307 26.731224552084214 27.48050210726601 24.46479339701671 92-93 21.425609620882884 26.586791934590124 27.43784635014082 24.549752094386164 94-95 21.280595132206678 26.365404706072614 27.569900541764376 24.784099619956336 96-97 21.28005477277114 26.069437839063063 27.675515836441384 24.974991551724408 98-99 21.128418764716535 26.285714285714285 27.43366884881103 25.152198100758145 100-101 21.23678099472566 25.76175328525572 27.93480709202002 25.066658627998606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1375.0 1 989.0 2 776.0 3 938.0 4 848.5 5 917.5 6 1420.5 7 2593.5 8 3856.0 9 4237.5 10 3730.5 11 3204.5 12 3309.0 13 3585.0 14 3818.5 15 4159.0 16 4562.5 17 4959.5 18 5367.5 19 6057.5 20 6846.5 21 7852.5 22 9530.0 23 11635.0 24 13855.0 25 17389.0 26 22915.0 27 29732.0 28 36077.0 29 42078.0 30 48671.0 31 57259.0 32 67803.5 33 73413.5 34 75906.0 35 81751.0 36 90004.0 37 97819.0 38 102916.0 39 106539.5 40 111146.5 41 116473.0 42 120278.0 43 124363.0 44 129673.5 45 134953.5 46 137527.5 47 140361.5 48 145782.0 49 145705.0 50 142347.0 51 140020.5 52 137286.0 53 136580.0 54 134158.5 55 124994.0 56 113603.0 57 101803.5 58 92901.0 59 83599.5 60 68323.5 61 55075.0 62 45587.5 63 34240.0 64 23990.0 65 17489.5 66 12497.5 67 9426.0 68 7123.5 69 5257.5 70 3887.5 71 2519.0 72 1699.0 73 1319.0 74 980.0 75 654.0 76 404.5 77 237.0 78 147.5 79 101.0 80 56.5 81 33.0 82 28.5 83 23.5 84 12.0 85 7.5 86 6.0 87 4.0 88 2.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23272181540611175 2 0.0 3 0.0 4 0.0 5 0.02771237094729216 6 0.0033637798535462 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003066214712655575 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.5875229642663075E-4 26-27 0.0014360752451678008 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006779310166377727 36-37 0.01740109193469092 38-39 0.014218438688643363 40-41 0.008732890004398789 42-43 0.008500012937614822 44-45 0.00403653582425544 46-47 4.916293632105985E-4 48-49 0.0018888917639144046 50-51 6.468807410665769E-5 52-53 0.0 54-55 0.0 56-57 0.001371387171061143 58-59 3.10502755711957E-4 60-61 0.002587522964266308 62-63 9.056330374932077E-4 64-65 0.0016301394674877738 66-67 0.005964240432633839 68-69 2.0700183714130463E-4 70-71 7.762568892798924E-5 72-73 0.0 74-75 4.5281651874660387E-4 76-77 0.05931896395580511 78-79 0.1411881905451911 80-81 8.4094496338655E-4 82-83 8.926954226718762E-4 84-85 0.0 86-87 0.10531218464563874 88-89 0.3494837891686289 90-91 0.38618780241674644 92-93 6.080678966025823E-4 94-95 3.234403705332885E-4 96-97 1.552513778559785E-4 98-99 0.0 100-101 0.0012031981783838333 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3864700.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.750774330812625 #Duplication Level Percentage of deduplicated Percentage of total 1 72.09474645086704 33.70485221761639 2 14.521631512084948 13.577950354734018 3 5.196366115171902 7.288024187720493 4 2.50641164761064 4.687067412702612 5 1.4137854764308433 3.3047782880399392 6 0.9014399703461748 2.528580997585707 7 0.6085112596834812 1.9913860805454666 8 0.4388764728594856 1.6414251953405463 9 0.334039296989401 1.4054936212057307 >10 1.7655435862902247 15.14527266018282 >50 0.1385146948168498 4.45670339547373 >100 0.0727644153304134 6.314435426450722 >500 0.004820389742902872 1.5441872813054303 >1k 0.0023824914425541713 1.6606475885664322 >5k 1.1081355546763574E-4 0.43436904402855214 >10k+ 5.540677773381787E-5 0.3148262485015817 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12154 0.31448754107692706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9959 0.2576914120112816 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6810 0.17621031386653555 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.762568892798923E-5 2 0.0 0.0 0.0 0.0 7.762568892798923E-5 3 0.0 0.0 0.0 0.0 7.762568892798923E-5 4 0.0 0.0 0.0 2.587522964266308E-5 7.762568892798923E-5 5 0.0 0.0 0.0 2.587522964266308E-5 7.762568892798923E-5 6 0.0 0.0 0.0 2.587522964266308E-5 7.762568892798923E-5 7 0.0 0.0 0.0 2.587522964266308E-5 1.293761482133154E-4 8 0.0 0.0 0.0 2.587522964266308E-5 1.293761482133154E-4 9 0.0 0.0 0.0 2.587522964266308E-5 1.8112660749864155E-4 10-11 0.0 0.0 0.0 3.8812844463994615E-5 1.8112660749864155E-4 12-13 0.0 0.0 0.0 1.0350091857065232E-4 2.716899112479623E-4 14-15 0.0 0.0 0.0 1.0350091857065232E-4 3.493156001759516E-4 16-17 0.0 0.0 0.0 1.940642223199731E-4 3.751908298186146E-4 18-19 0.0 0.0 0.0 2.0700183714130463E-4 3.881284446399462E-4 20-21 0.0 0.0 0.0 2.716899112479623E-4 4.0106605946127775E-4 22-23 0.0 0.0 0.0 5.0456697803193E-4 4.7869174838926695E-4 24-25 0.0 0.0 0.0 0.0011643853339198386 5.692550521385878E-4 26-27 0.0 0.0 0.0 0.002496959660516987 5.951302817812508E-4 28-29 0.0 0.0 0.0 0.008008383574404223 5.951302817812508E-4 30-31 0.0 0.0 0.0 0.025668227805521776 5.951302817812508E-4 32-33 0.0 0.0 0.0 0.06724972184128135 5.951302817812508E-4 34-35 0.0 0.0 0.0 0.13390431340078143 5.951302817812508E-4 36-37 0.0 0.0 0.0 0.24265790358889433 5.951302817812508E-4 38-39 0.0 0.0 0.0 0.3992289181566486 6.210055114239138E-4 40-41 0.0 0.0 0.0 0.5672109089968174 6.46880741066577E-4 42-43 1.293761482133154E-5 0.0 0.0 0.752244676171501 6.46880741066577E-4 44-45 2.587522964266308E-5 0.0 0.0 0.9820296530131705 6.598183558879085E-4 46-47 2.587522964266308E-5 0.0 0.0 1.2522834890159649 6.856935855305715E-4 48-49 2.587522964266308E-5 0.0 0.0 1.5293942608740654 7.503816596372293E-4 50-51 2.587522964266308E-5 0.0 0.0 1.824747069630243 7.503816596372293E-4 52-53 2.587522964266308E-5 0.0 0.0 2.1249256087147774 7.891945041012239E-4 54-55 2.587522964266308E-5 0.0 0.0 2.467138458353818 8.280073485652185E-4 56-57 2.587522964266308E-5 0.0 0.0 2.861192330581934 8.280073485652185E-4 58-59 2.587522964266308E-5 0.0 0.0 3.2769555204802443 8.4094496338655E-4 60-61 2.587522964266308E-5 0.0 0.0 3.7228892281418995 8.538825782078816E-4 62-63 2.587522964266308E-5 0.0 0.0 4.173351100991021 8.538825782078816E-4 64-65 2.587522964266308E-5 0.0 0.0 4.6612026806737905 8.538825782078816E-4 66-67 2.587522964266308E-5 0.0 0.0 5.2116852537066265 8.538825782078816E-4 68-69 2.587522964266308E-5 0.0 0.0 5.819196832871892 8.538825782078816E-4 70-71 2.587522964266308E-5 0.0 0.0 6.458819572023701 8.797578078505447E-4 72-73 2.587522964266308E-5 0.0 0.0 7.1105648562631 0.0010220715708851916 74-75 2.587522964266308E-5 0.0 0.0 7.7861671022330325 0.0010350091857065232 76-77 2.587522964266308E-5 0.0 0.0 8.507840194581727 0.0010350091857065232 78-79 2.587522964266308E-5 0.0 0.0 9.309454808911429 0.0010738220301705179 80-81 2.587522964266308E-5 0.0 0.0 10.14896369705281 0.001151447719098507 82-83 2.587522964266308E-5 0.0 0.0 11.01252361114705 0.0012420110228478277 84-85 2.587522964266308E-5 0.0 0.0 11.896007452066137 0.0012420110228478277 86-87 2.587522964266308E-5 0.0 0.0 12.814992108054959 0.001267886252490491 88-89 2.587522964266308E-5 0.0 0.0 13.774613812197583 0.001293761482133154 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11180 0.0 32.762653 1 GTATCAA 16600 0.0 31.550728 1 ATCAACG 20480 0.0 25.467424 3 TATCAAC 20560 0.0 25.437578 2 CAACGCA 20800 0.0 25.029982 5 TCAACGC 20950 0.0 24.828117 4 AACGCAG 21375 0.0 24.39381 6 GAGTACT 13655 0.0 21.682203 12-13 TACATGG 11355 0.0 21.56651 2 ACGCAGA 24115 0.0 21.562548 7 GTACATG 11750 0.0 21.53793 1 CGCAGAG 24065 0.0 21.52849 8 ACATGGG 11790 0.0 19.88522 3 AGTACTT 14280 0.0 19.869343 12-13 GTACTTT 14575 0.0 19.74389 14-15 GCAGAGT 25805 0.0 19.70914 9 AGAGTAC 20250 0.0 19.681799 10-11 CATGGGG 6905 0.0 18.00756 4 CAGAGTA 24665 0.0 16.966722 10-11 TATTCCG 285 8.673487E-7 16.65223 5 >>END_MODULE