##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938781_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2985582 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.710329175350065 33.0 31.0 34.0 30.0 34.0 2 31.764863936076786 33.0 31.0 34.0 30.0 34.0 3 31.750134814585564 33.0 31.0 34.0 30.0 34.0 4 35.33838929897085 37.0 35.0 37.0 33.0 37.0 5 35.400879627489715 37.0 35.0 37.0 33.0 37.0 6 35.51467687037234 37.0 35.0 37.0 33.0 37.0 7 35.455387592770855 37.0 35.0 37.0 33.0 37.0 8 35.436765427980205 37.0 35.0 37.0 33.0 37.0 9 36.98752136099427 39.0 37.0 39.0 33.0 39.0 10-11 36.893716200057476 39.0 37.0 39.0 33.0 39.0 12-13 36.814391297911094 39.0 37.0 39.0 32.0 39.0 14-15 37.903438257599355 40.0 37.5 41.0 33.0 41.0 16-17 37.883193796050485 40.0 37.5 41.0 33.0 41.0 18-19 37.965041991812654 40.0 38.0 41.0 33.0 41.0 20-21 37.9100074960259 40.0 38.0 41.0 33.0 41.0 22-23 37.820714855595995 40.0 37.5 41.0 32.0 41.0 24-25 37.80935861081692 40.0 37.0 41.0 32.0 41.0 26-27 37.70482405105604 40.0 37.0 41.0 32.0 41.0 28-29 37.604401922305264 40.0 37.0 41.0 32.0 41.0 30-31 37.367420154596324 40.0 37.0 41.0 32.0 41.0 32-33 37.0836905501172 39.5 36.0 41.0 31.5 41.0 34-35 36.73143661771809 39.0 35.0 41.0 30.5 41.0 36-37 36.43956873400228 39.0 35.0 41.0 30.0 41.0 38-39 36.25142250321713 39.0 35.0 41.0 30.0 41.0 40-41 36.09947373744885 39.0 35.0 41.0 30.0 41.0 42-43 35.76957273322253 39.0 35.0 41.0 28.5 41.0 44-45 35.47461684187539 39.0 35.0 41.0 26.5 41.0 46-47 35.0588675842767 39.0 35.0 41.0 23.5 41.0 48-49 34.67631821869237 39.0 35.0 41.0 21.0 41.0 50-51 33.526497011302986 38.0 34.0 40.0 16.5 40.5 52-53 33.663227638698245 38.0 33.5 39.5 15.0 40.5 54-55 34.23339017317227 39.0 35.0 40.5 15.0 41.0 56-57 34.18684430707313 39.0 34.5 41.0 13.5 41.0 58-59 34.0282378779079 39.0 34.0 41.0 10.5 41.0 60-61 33.76715360690143 38.5 34.0 40.5 8.0 41.0 62-63 33.4669334153274 38.0 34.0 40.0 6.5 41.0 64-65 33.12356518762506 38.0 33.0 40.0 2.0 41.0 66-67 32.72791921307135 37.5 33.0 40.0 2.0 41.0 68-69 32.32764600000938 37.0 32.0 39.5 2.0 41.0 70-71 31.905768289063907 36.0 32.0 39.0 2.0 41.0 72-73 31.465495504729063 36.0 31.0 39.0 2.0 40.0 74-75 30.986985619554247 35.0 31.0 38.0 2.0 40.0 76-77 30.533927555833337 35.0 31.0 37.0 2.0 39.5 78-79 30.068667013667685 35.0 30.0 37.0 2.0 39.0 80-81 29.571172555300777 35.0 29.5 36.0 2.0 39.0 82-83 29.05341739064611 34.5 29.0 36.0 2.0 37.5 84-85 28.574961933720125 34.0 29.0 35.5 2.0 37.0 86-87 28.1986962675954 34.0 29.0 35.0 2.0 37.0 88-89 27.764683401762202 34.0 27.5 35.0 2.0 36.0 90-91 27.29322473809127 34.0 26.0 35.0 2.0 36.0 92-93 26.826642845515547 34.0 25.5 35.0 2.0 36.0 94-95 26.307359503105257 34.0 24.5 35.0 2.0 35.0 96-97 25.72986406000572 33.5 21.5 35.0 2.0 35.0 98-99 25.10140367941661 33.0 10.0 35.0 2.0 35.0 100-101 23.030389552187813 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2455671722875863 1101 2 0.16604249342720223 1101 3 0.21224477818027765 1101 4 0.1750723237944669 1101 5 0.049941187866430425 1101 6 -0.001382806936248926 1101 7 0.21399016300947693 1101 8 0.25290195071589494 1101 9 0.33259831437912624 1101 10-11 0.3346951740531523 1101 12-13 0.34444309358578806 1101 14-15 0.44692702652879035 1101 16-17 0.43554282960310786 1101 18-19 0.3047258984706289 1101 20-21 0.3929630044388688 1101 22-23 0.3298302042861678 1101 24-25 0.46683359962751325 1101 26-27 0.4098408123884667 1101 28-29 0.33878093191691505 1101 30-31 0.3803217578976046 1101 32-33 0.35805485310424245 1101 34-35 0.4486850195975052 1101 36-37 0.42837755271659006 1101 38-39 0.48929777058331325 1101 40-41 0.5263424106059702 1101 42-43 0.444736909655596 1101 44-45 0.6579967362574308 1101 46-47 0.6454751803345289 1101 48-49 0.5127962827322889 1101 50-51 0.566244827233298 1101 52-53 0.5622999652106273 1101 54-55 0.575824688285735 1101 56-57 0.6598913627328997 1101 58-59 0.5168286175210071 1101 60-61 0.493655909508 1101 62-63 0.601237788046511 1101 64-65 0.5286127292559826 1101 66-67 0.43845170398333266 1101 68-69 0.3147523965603085 1101 70-71 0.13176257727242202 1101 72-73 0.0283273067979799 1101 74-75 0.05496441060775581 1101 76-77 0.030853043102894162 1101 78-79 -0.06184114000637919 1101 80-81 -0.011122312813007795 1101 82-83 -0.004877570256486052 1101 84-85 0.07862978130176046 1101 86-87 0.07060064089918328 1101 88-89 -0.06308842596526887 1101 90-91 -0.04083504823859485 1101 92-93 -0.24990455874806017 1101 94-95 -0.2840095199500041 1101 96-97 -0.2560327244242373 1101 98-99 -0.40700388260447085 1101 100-101 -0.12682936600371875 1102 1 0.31776097275645654 1102 2 0.3020693233308229 1102 3 0.3599514726319555 1102 4 0.2780517455188729 1102 5 0.21733894769971585 1102 6 0.2943283157519545 1102 7 0.3503902672032737 1102 8 0.24531638155725943 1102 9 0.372784821281968 1102 10-11 0.39329767470436394 1102 12-13 0.3226376755081617 1102 14-15 0.29829001168117486 1102 16-17 0.3897543430180619 1102 18-19 0.4227044084992855 1102 20-21 0.3556462552854498 1102 22-23 0.42564955828460427 1102 24-25 0.524365378736654 1102 26-27 0.46068725677503153 1102 28-29 0.4612834065196836 1102 30-31 0.4603743757668468 1102 32-33 0.5156767598507841 1102 34-35 0.5120696249634875 1102 36-37 0.48028885774393615 1102 38-39 0.49118133401129427 1102 40-41 0.49543253824338507 1102 42-43 0.3860877822786719 1102 44-45 0.6169336990081433 1102 46-47 0.6062463447002457 1102 48-49 0.7063947458737374 1102 50-51 0.6043422484367369 1102 52-53 0.6054944168907497 1102 54-55 0.603073450984354 1102 56-57 0.48875004989101 1102 58-59 0.33966985221753987 1102 60-61 0.3525832343321724 1102 62-63 0.3269516458219712 1102 64-65 0.39323854327004426 1102 66-67 0.3271729855670742 1102 68-69 0.15031621526308925 1102 70-71 0.18274343166158147 1102 72-73 0.20949909632649977 1102 74-75 0.15899762238165493 1102 76-77 0.20861365784631758 1102 78-79 -0.08463001731817599 1102 80-81 -0.07275607160435982 1102 82-83 -0.05573352232733342 1102 84-85 -0.12875768939373344 1102 86-87 -0.2592597394081899 1102 88-89 -0.210056777098373 1102 90-91 -0.1859431493065813 1102 92-93 -0.20989856760452952 1102 94-95 -0.25350691510499246 1102 96-97 -0.27141746003247036 1102 98-99 -0.2731315200100468 1102 100-101 -0.12593212713943203 1103 1 0.055680089587138326 1103 2 0.11438247339036067 1103 3 0.12998229555092777 1103 4 0.21463073765220742 1103 5 0.2047800851937751 1103 6 0.16834281759918923 1103 7 0.06512547632750909 1103 8 0.09496794302432932 1103 9 0.27031644125758447 1103 10-11 0.23095442493380602 1103 12-13 0.27376371537423694 1103 14-15 0.2818926084051512 1103 16-17 0.3111161741398405 1103 18-19 0.39603197738993146 1103 20-21 0.3208753594141456 1103 22-23 0.4414695411313332 1103 24-25 0.32106150254577415 1103 26-27 0.3516093545466248 1103 28-29 0.30902279598956994 1103 30-31 0.25385903611885396 1103 32-33 0.2543828109622339 1103 34-35 0.2736778127979065 1103 36-37 0.32871688633000673 1103 38-39 0.23765836910317972 1103 40-41 0.36976560809814174 1103 42-43 0.33155790309513833 1103 44-45 0.47821643053400464 1103 46-47 0.28072567317620667 1103 48-49 0.4118508023328502 1103 50-51 0.46663657981061846 1103 52-53 0.33937012647488984 1103 54-55 0.4617535251793825 1103 56-57 0.46064151624096183 1103 58-59 0.4461066448616009 1103 60-61 0.37889858151782363 1103 62-63 0.4272383697612696 1103 64-65 0.2550801693878313 1103 66-67 0.18497311431846697 1103 68-69 0.15534652124117088 1103 70-71 0.0575563270704329 1103 72-73 0.2513089827828523 1103 74-75 0.2001620966756903 1103 76-77 0.2961255118357222 1103 78-79 0.16587310877363848 1103 80-81 0.023405884194392712 1103 82-83 0.09582106917731537 1103 84-85 0.02635863758589707 1103 86-87 -0.009991350827537104 1103 88-89 0.00809769044231956 1103 90-91 -0.029890408093812937 1103 92-93 -0.014108223551083654 1103 94-95 -0.07000493086685111 1103 96-97 -0.0736407133070216 1103 98-99 -0.08531619879876473 1103 100-101 0.2961203803040071 1104 1 0.15644857710061189 1104 2 0.17933169136356142 1104 3 0.25765279561226606 1104 4 0.22034242345159782 1104 5 0.2263061059937712 1104 6 0.2720231596738074 1104 7 0.25331851882707923 1104 8 0.21465258358672656 1104 9 0.2787894894360079 1104 10-11 0.2499081070489595 1104 12-13 0.28352509441893403 1104 14-15 0.4299487844677188 1104 16-17 0.38874031734624737 1104 18-19 0.3233568466553365 1104 20-21 0.43794355367462146 1104 22-23 0.4949644369903723 1104 24-25 0.3774089833555152 1104 26-27 0.4632280652484013 1104 28-29 0.4010267741958984 1104 30-31 0.37084747328197665 1104 32-33 0.427918153004903 1104 34-35 0.4427840358438644 1104 36-37 0.3676155441928586 1104 38-39 0.3906525307332842 1104 40-41 0.2444875703457754 1104 42-43 0.3177364930823856 1104 44-45 0.4724563767227039 1104 46-47 0.4083086471849171 1104 48-49 0.4642737076690082 1104 50-51 0.5526029520129967 1104 52-53 0.5187269847895664 1104 54-55 0.501130036444799 1104 56-57 0.4912909846126041 1104 58-59 0.4880962818270973 1104 60-61 0.4564090097739637 1104 62-63 0.5327071378718671 1104 64-65 0.46357981997280717 1104 66-67 0.5043660180795229 1104 68-69 0.4320400563802167 1104 70-71 0.41866820741949695 1104 72-73 0.5623702458821604 1104 74-75 0.3227488179522595 1104 76-77 0.2896883685106566 1104 78-79 0.5111693384119249 1104 80-81 0.4887300920961444 1104 82-83 0.5237692717981837 1104 84-85 0.6170066198315745 1104 86-87 0.6647736789996124 1104 88-89 0.4991696571571076 1104 90-91 0.5014099608939695 1104 92-93 0.4982626473275005 1104 94-95 0.5195363511686573 1104 96-97 0.6267994606624434 1104 98-99 0.6112970755138996 1104 100-101 0.6933188699689765 1105 1 0.19152860479447398 1105 2 0.22477252097541012 1105 3 0.29022549443372014 1105 4 0.31404202076416254 1105 5 0.23728278566257188 1105 6 0.23173014072215636 1105 7 0.16579457072297288 1105 8 0.19069258432470804 1105 9 0.2457305457840846 1105 10-11 0.2804940721247746 1105 12-13 0.3584251872496935 1105 14-15 0.37307027721750075 1105 16-17 0.30270811885930726 1105 18-19 0.30780854309873007 1105 20-21 0.2753583212432744 1105 22-23 0.37744315194456846 1105 24-25 0.34974406794706425 1105 26-27 0.3359881953085804 1105 28-29 0.3044944305395063 1105 30-31 0.2920614273190978 1105 32-33 0.29163887514280873 1105 34-35 0.30011614907408557 1105 36-37 0.322867910843307 1105 38-39 0.29030190281472557 1105 40-41 0.29880522052332736 1105 42-43 0.2595633559365851 1105 44-45 0.4150986646320831 1105 46-47 0.46919005636758726 1105 48-49 0.3678004149637033 1105 50-51 0.4503756812277899 1105 52-53 0.4681870175522249 1105 54-55 0.5389059555033668 1105 56-57 0.37085417265025455 1105 58-59 0.35608481586811536 1105 60-61 0.44399750730413956 1105 62-63 0.3239333803330098 1105 64-65 0.3301953738840808 1105 66-67 0.3243456433772707 1105 68-69 0.37470831942532357 1105 70-71 0.3822474257572672 1105 72-73 0.34262345004866646 1105 74-75 0.4058101406353032 1105 76-77 0.38523596321308773 1105 78-79 0.42456932831318284 1105 80-81 0.5573584034404355 1105 82-83 0.359517746547926 1105 84-85 0.19883363805107024 1105 86-87 0.2685262607338963 1105 88-89 0.09770220764354676 1105 90-91 0.24669249997077358 1105 92-93 0.2858695459351388 1105 94-95 0.310319681151924 1105 96-97 0.447020994584026 1105 98-99 0.5897233901227992 1105 100-101 0.9970866119577124 1106 1 0.15281482119040746 1106 2 0.08479484452438157 1106 3 0.11307863397965079 1106 4 0.11704097583208295 1106 5 0.1419788054212887 1106 6 0.10588992033648026 1106 7 0.09599643260195023 1106 8 0.18780477203250712 1106 9 0.15851054008445686 1106 10-11 0.215776678755347 1106 12-13 0.12973776443217844 1106 14-15 0.20040665035324423 1106 16-17 0.19699424026141799 1106 18-19 0.2385879674361462 1106 20-21 0.24169654675861807 1106 22-23 0.1822534017783326 1106 24-25 0.13346996326387028 1106 26-27 -0.019761068489273725 1106 28-29 0.00322293818651076 1106 30-31 0.11683272968444669 1106 32-33 0.10859717285345738 1106 34-35 0.07056903213668875 1106 36-37 0.07191987246580567 1106 38-39 0.10853288030118335 1106 40-41 0.2316621598222781 1106 42-43 0.14881527956154628 1106 44-45 0.08365190867122152 1106 46-47 0.12155041544425416 1106 48-49 0.1277555303812008 1106 50-51 0.14472758585399248 1106 52-53 0.06550059216986881 1106 54-55 0.05003471963370032 1106 56-57 -0.0015945306840308149 1106 58-59 -0.11108674298056087 1106 60-61 0.01607597220392165 1106 62-63 -0.08389387339549614 1106 64-65 -0.18792018610452033 1106 66-67 -0.2525953179602425 1106 68-69 -0.061166098737501784 1106 70-71 -0.1559239431037618 1106 72-73 -0.052249495709858707 1106 74-75 -0.1255026740317433 1106 76-77 -0.20827548354287373 1106 78-79 4.660700249701222E-4 1106 80-81 -0.031050212499525998 1106 82-83 -0.11400296210601013 1106 84-85 -0.0840128519270813 1106 86-87 -0.06389465690645935 1106 88-89 -0.0867530027677752 1106 90-91 -0.00733975043295132 1106 92-93 0.10193870144003014 1106 94-95 0.030526530206735458 1106 96-97 0.07178232259770922 1106 98-99 0.1339710390256208 1106 100-101 0.47007345531289246 1107 1 -0.015089957384770258 1107 2 0.04547458079837696 1107 3 0.052494585902167046 1107 4 0.03743073019348486 1107 5 -0.07147705548074867 1107 6 0.009713947409238699 1107 7 -0.056528218777941674 1107 8 0.06498255370004813 1107 9 0.022197452973188092 1107 10-11 -0.14842280779578942 1107 12-13 -0.06603590964448358 1107 14-15 0.027126995764298556 1107 16-17 0.00935639674662525 1107 18-19 0.0472098240205483 1107 20-21 0.07544939114860938 1107 22-23 0.02106109184192917 1107 24-25 0.014967425192800476 1107 26-27 0.04895910188238872 1107 28-29 -0.0864062700520094 1107 30-31 0.052917973864381906 1107 32-33 0.16239756960938934 1107 34-35 0.135650717182493 1107 36-37 0.24555952472089615 1107 38-39 0.2799075229291148 1107 40-41 0.1926257516304375 1107 42-43 0.15661415930949119 1107 44-45 0.259259003600917 1107 46-47 0.363978795163014 1107 48-49 0.48870891155862495 1107 50-51 0.5759131859473428 1107 52-53 0.4798471118034584 1107 54-55 0.363360759659372 1107 56-57 0.4173569834066413 1107 58-59 0.3866409540846121 1107 60-61 0.2762813517476488 1107 62-63 0.36028285803574533 1107 64-65 0.3284856718801876 1107 66-67 0.24541740156831793 1107 68-69 0.4008357683492676 1107 70-71 0.5355084625208342 1107 72-73 0.3001707155043327 1107 74-75 0.3149188791510582 1107 76-77 0.319190529643425 1107 78-79 0.22565832161490817 1107 80-81 0.2007284793728097 1107 82-83 0.2837743294512549 1107 84-85 0.140838364596636 1107 86-87 0.13037513713053528 1107 88-89 0.206283055345299 1107 90-91 0.26652074317810914 1107 92-93 0.4120431416057322 1107 94-95 0.30857746666744035 1107 96-97 0.1800835653773305 1107 98-99 0.23966186112726007 1107 100-101 0.5520566515320411 1108 1 0.022585013120565378 1108 2 -0.07841808015862384 1108 3 -0.05592961139531738 1108 4 -0.024969325112550678 1108 5 0.06290958584487072 1108 6 0.08343848028289358 1108 7 0.037194206119750106 1108 8 0.04105441981410962 1108 9 -0.002685616693149484 1108 10-11 0.08029281151281253 1108 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-1.961626321166122 2316 68-69 -2.170051664458043 2316 70-71 -1.9384081091839853 2316 72-73 -2.0327958636823347 2316 74-75 -1.9863618107898944 2316 76-77 -2.01511026343184 2316 78-79 -1.7585899546837638 2316 80-81 -1.7416614799950345 2316 82-83 -1.7638471641546474 2316 84-85 -1.8909475223469734 2316 86-87 -1.887731352504236 2316 88-89 -1.9144754595665887 2316 90-91 -2.1316549822728135 2316 92-93 -2.1674144536150983 2316 94-95 -2.1194077883482727 2316 96-97 -2.1494996384625793 2316 98-99 -2.4767835156698226 2316 100-101 -2.8187433012748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8264.0 3 1922.0 4 1836.0 5 2100.0 6 2428.0 7 3172.0 8 3871.0 9 5067.0 10 6995.0 11 10233.0 12 15579.0 13 22467.0 14 25274.0 15 28470.0 16 39208.0 17 53672.0 18 40755.0 19 22463.0 20 20298.0 21 20101.0 22 21682.0 23 23920.0 24 27043.0 25 31043.0 26 35730.0 27 41922.0 28 48019.0 29 57111.0 30 68075.0 31 81483.0 32 99574.0 33 124504.0 34 163248.0 35 223513.0 36 318877.0 37 524628.0 38 626783.0 39 134215.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13053916161429 22.7715198995161 15.599528444941853 19.498412493927756 2 13.645653478524707 23.839402158425337 42.06622791871876 20.4487164443312 3 17.746924030544136 30.979744294429494 32.397060371536746 18.87627130348962 4 10.268230604756928 19.465268899513188 43.33564349829386 26.93085699743602 5 9.627006849101448 41.57166136067832 36.541799036906944 12.259532753313295 6 23.792185790042616 42.44738734427974 19.844133981244877 13.916292884432766 7 23.294693925694787 36.81898923652404 24.81962859121812 15.06668824656305 8 23.5640534501455 34.85237406523234 22.469442027985057 19.114130456637103 9 26.043006738631636 18.1063067814916 24.585097802145143 31.26558867773162 10-11 21.879190290346962 28.900120986976262 29.98463075012763 19.23605797254914 12-13 24.572204403572695 28.132853140183716 29.57726962139382 17.717672834849772 14-15 21.761742849535935 28.554790525663385 30.249173585083327 19.434293039717343 16-17 17.418829270286945 31.91239247858619 31.028194410980863 19.640583840145997 18-19 17.262133856334874 30.70994778083197 34.56057733471959 17.467341028113566 20-21 18.988762858838097 28.90465227999371 34.09373058910917 18.012854272059027 22-23 18.455894798406625 28.815593887260544 34.105335659378504 18.623175654954323 24-25 18.693178256478195 29.72656507961623 34.13563570794768 17.4446209559579 26-27 18.20781664897221 28.799970365831136 35.546219078974055 17.445993906222608 28-29 17.190455456416746 29.617957476790423 35.37393588904319 17.817651177749642 30-31 17.932232712886737 29.366232185355752 34.6431340032597 18.05840109849781 32-33 17.79203368334698 29.796132842654043 34.6430066891162 17.768826784882773 34-35 18.5710683904896 29.247145770025213 34.52783026450455 17.653955574980635 36-37 18.636897957150747 28.735124871823437 34.68907991184212 17.9388972591837 38-39 17.712548836470667 30.18576700343588 34.46024512779241 17.641439032301044 40-41 17.635604450236727 29.969553508073965 34.812804445998474 17.582037595690835 42-43 17.431133391689187 30.123682892521202 33.5095056503282 18.935678065461406 44-45 17.93295395695219 29.624856858942582 33.41308515374435 19.02910403036088 46-47 18.854503134694443 30.194976614718446 32.76530491721856 18.185215333368554 48-49 18.438739694141887 30.358499310222275 32.49756751963065 18.705193476005192 50-51 17.63736296094382 30.555105454135507 32.94580863033717 18.861722954583506 52-53 18.13183188842819 31.398534677723333 31.859300118464272 18.6103333153842 54-55 17.874176017488107 30.421408259031818 32.280074888457285 19.42434083502279 56-57 18.49014345509471 30.489733669908947 31.65792424626273 19.362198628733612 58-59 18.803148128402146 29.954344872636224 32.13112873750269 19.11137826145894 60-61 18.33914235034005 30.829621783393442 32.34221919581684 18.489016670449672 62-63 17.844434096278768 30.53319933056939 32.72659735040601 18.895769222745837 64-65 17.75599957185585 30.80954923018201 32.288969027540574 19.14548217042157 66-67 17.99847420923866 30.306990918801713 31.212771403860458 20.481763468099174 68-69 17.54174003875999 30.374144656160784 31.879957139813015 20.204158165266215 70-71 18.543050704707525 30.138009536807903 31.701260359299095 19.617679399185477 72-73 18.297615815955336 29.31176285760783 32.28499605095904 20.10562527547779 74-75 17.46969085794187 31.084929545538305 31.32852008523902 20.116859511280808 76-77 17.776576086069543 29.758661307959166 31.919771487142285 20.54499111882901 78-79 17.69513268166174 29.679265953565498 31.938703644176748 20.686897720596015 80-81 18.239011907733733 29.97770453990339 31.17885689255048 20.60442665981239 82-83 18.138708094359014 29.488832312496392 31.771663392485067 20.600796200659524 84-85 17.603196308007863 29.57695250490065 31.45832814612386 21.36152304096763 86-87 17.586595446084598 29.80746921003134 31.55143349452579 21.054501849358275 88-89 18.092558383257213 29.05357720395092 31.278301462934277 21.57556294985759 90-91 17.991542589702853 28.477785625229597 31.71845227607795 21.812219508989592 92-93 18.619291045146593 29.122621964733497 31.411479594026137 20.84660739609377 94-95 18.215151805833933 28.124426762793814 31.590961490240034 22.06945994113222 96-97 17.908876611380855 27.907410782609514 31.837531645324635 22.34618096068499 98-99 18.204094133604155 28.327116714528884 30.90891650771026 22.5598726441567 100-101 18.14753325751614 27.33058365609483 31.63634736119657 22.885535725192458 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5777.0 1 3390.0 2 1262.5 3 1511.0 4 1262.0 5 1363.5 6 2503.5 7 4763.5 8 7340.5 9 8426.0 10 7774.0 11 7274.5 12 7791.5 13 8483.5 14 9034.0 15 9464.5 16 9971.0 17 10563.5 18 11084.5 19 11727.0 20 12810.0 21 14255.5 22 16052.5 23 18196.5 24 20232.0 25 23536.0 26 31209.0 27 39298.5 28 42213.5 29 47455.0 30 58960.0 31 69695.0 32 84515.0 33 105324.5 34 125122.5 35 137984.5 36 154467.5 37 168794.0 38 162312.0 39 151705.0 40 139397.5 41 125337.0 42 111916.0 43 104914.5 44 102785.5 45 96711.5 46 89486.5 47 93369.0 48 125933.0 49 118749.5 50 72409.0 51 46388.5 52 31654.5 53 24677.0 54 19892.0 55 15855.5 56 12483.0 57 9649.5 58 7305.5 59 5590.5 60 4308.0 61 3328.0 62 2559.5 63 2057.5 64 1566.0 65 1086.5 66 754.0 67 647.5 68 533.0 69 401.5 70 328.5 71 258.5 72 222.0 73 194.5 74 171.0 75 150.5 76 118.5 77 87.5 78 71.5 79 59.5 80 41.0 81 29.0 82 23.0 83 20.5 84 14.0 85 6.5 86 4.5 87 4.0 88 3.5 89 2.0 90 2.5 91 2.0 92 0.5 93 0.0 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16097363931052638 2 0.0955257634859803 3 0.08775508426832691 4 0.10420078899189505 5 0.12496725931493424 6 0.04099703173451608 7 0.044312968124807825 8 0.04749492728720899 9 0.04839927357547038 10-11 0.046104913547844274 12-13 0.050408931993829 14-15 0.04881795241262842 16-17 0.0471934785244552 18-19 0.05106207097979557 20-21 0.053373178160907996 22-23 0.06621824488491691 24-25 0.08236250084573124 26-27 0.08504204540354277 28-29 0.0744578444001873 30-31 0.06266784834581667 32-33 0.05210039449594753 34-35 0.2982165621309346 36-37 0.4721022567794152 38-39 0.4494266109589353 40-41 0.4240881677341302 42-43 0.45507040168382584 44-45 0.4643315775617618 46-47 0.4635947028083637 48-49 0.48014089045284974 50-51 0.46990837967270704 52-53 0.47635603376494096 54-55 0.4676642611055399 56-57 0.4452398225873548 58-59 0.42340153444119105 60-61 0.39379256707737387 62-63 0.4523071213585827 64-65 0.4896867679400532 66-67 0.49114377029336326 68-69 0.5021801444408494 70-71 0.49844552921339963 72-73 0.4892680891028952 74-75 0.48256922770836647 76-77 0.46374542718974054 78-79 0.4378543278998869 80-81 0.443062692634133 82-83 0.4552713675256617 84-85 0.45311098472592615 86-87 0.46475025639891987 88-89 0.47645651668585887 90-91 0.4803586034481719 92-93 0.4825022390944211 94-95 0.4862368543218709 96-97 0.4878948225170168 98-99 0.4816313871131324 100-101 0.4675972724915946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2985582.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.330034628184688 #Duplication Level Percentage of deduplicated Percentage of total 1 72.68253374838835 18.41051096710879 2 12.177227080147848 6.168991672308267 3 4.905767200434896 3.727897591944858 4 2.6252488585321214 2.659905779768839 5 1.5951738464844243 2.020290438471252 6 1.070587319603463 1.6270808326830688 7 0.7424969356732907 1.3165231164347833 8 0.5366255226444064 1.087419445676042 9 0.4216194960574347 0.961167279154734 >10 2.4650250593662792 11.851101854458138 >50 0.3183091479129865 5.737989324500585 >100 0.3851170136599341 20.69107495961447 >500 0.04902273199953502 8.454676385060406 >1k 0.02350953905154813 10.108839695259414 >5k 9.350384850047551E-4 1.4954429418514552 >10k+ 8.014615585755044E-4 3.6810877157049884 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26530 0.8886039639842416 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24463 0.8193712314717867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19307 0.6466745847208349 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14194 0.475418193169707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13392 0.4485557589776466 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10909 0.36538939476457183 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9048 0.30305648948848163 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6712 0.22481378840038557 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6181 0.2070283113979117 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5822 0.19500385519473257 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5803 0.19436746336225233 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5561 0.1862618410748725 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5071 0.16984963065827702 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4964 0.16626573981220413 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4915 0.16462451877054457 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4801 0.16080616777566317 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4429 0.14834628558183965 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4328 0.14496336057760262 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3733 0.12503424792887952 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3608 0.12084745955729904 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3438 0.11515342737194958 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3401 0.11391413801396177 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3285 0.11002879840513508 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3267 0.10942590087962749 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3092 0.10356439715941482 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3082 0.10322945408968837 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 3006 0.10068388675976744 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.349430697264386E-5 0.0 14-15 0.0 0.0 0.0 3.349430697264386E-5 3.349430697264386E-5 16-17 0.0 0.0 0.0 6.698861394528772E-5 3.349430697264386E-5 18-19 0.0 0.0 0.0 1.5072438137689738E-4 3.349430697264386E-5 20-21 0.0 0.0 0.0 3.349430697264386E-4 3.349430697264386E-5 22-23 3.349430697264386E-5 0.0 0.0 9.545877487203501E-4 3.349430697264386E-5 24-25 1.0048292091793158E-4 0.0 0.0 0.002394842948544036 3.349430697264386E-5 26-27 1.0048292091793158E-4 0.0 0.0 0.005225111887732442 3.349430697264386E-5 28-29 1.0048292091793158E-4 0.0 0.0 0.01361543578437973 3.349430697264386E-5 30-31 1.0048292091793158E-4 0.0 0.0 0.04071233012524861 3.349430697264386E-5 32-33 1.0048292091793158E-4 0.0 0.0 0.09627938539286478 3.349430697264386E-5 34-35 1.3397722789057544E-4 0.0 0.0 0.1769504237364775 3.349430697264386E-5 36-37 1.3397722789057544E-4 0.0 0.0 0.29707775569386474 3.349430697264386E-5 38-39 1.674715348632193E-4 0.0 0.0 0.4824854919409348 3.349430697264386E-5 40-41 2.0096584183586317E-4 0.0 0.0 0.7069140958111351 3.349430697264386E-5 42-43 2.0096584183586317E-4 0.0 0.0 0.9242251594496483 3.349430697264386E-5 44-45 2.0096584183586317E-4 0.0 0.0 1.1742434138469484 3.349430697264386E-5 46-47 2.0096584183586317E-4 0.0 0.0 1.4781037667027737 3.349430697264386E-5 48-49 2.0096584183586317E-4 0.0 0.0 1.8041206036210027 3.349430697264386E-5 50-51 2.0096584183586317E-4 0.0 0.0 2.17394799405945 3.349430697264386E-5 52-53 2.0096584183586317E-4 0.0 0.0 2.526860759476712 3.349430697264386E-5 54-55 2.0096584183586317E-4 0.0 0.0 2.8930875119156 3.349430697264386E-5 56-57 2.0096584183586317E-4 0.0 0.0 3.3303556894434654 3.349430697264386E-5 58-59 2.0096584183586317E-4 0.0 0.0 3.7967974083445037 3.349430697264386E-5 60-61 2.0096584183586317E-4 0.0 0.0 4.322306337591799 3.349430697264386E-5 62-63 3.0144876275379475E-4 0.0 0.0 4.849992396792317 3.349430697264386E-5 64-65 4.0193168367172634E-4 0.0 0.0 5.369706810933346 3.349430697264386E-5 66-67 4.0193168367172634E-4 0.0 0.0 5.945792143709333 3.349430697264386E-5 68-69 4.0193168367172634E-4 0.0 0.0 6.582284459110485 3.349430697264386E-5 70-71 4.0193168367172634E-4 0.0 0.0 7.296935739832301 3.349430697264386E-5 72-73 4.0193168367172634E-4 3.349430697264386E-5 0.0 8.004486227475915 3.349430697264386E-5 74-75 4.0193168367172634E-4 3.349430697264386E-5 0.0 8.719120761044245 3.349430697264386E-5 76-77 4.1867883715804825E-4 3.349430697264386E-5 0.0 9.429786219236316 3.349430697264386E-5 78-79 4.3542599064437016E-4 3.349430697264386E-5 0.0 10.261399619906605 3.349430697264386E-5 80-81 4.3542599064437016E-4 3.349430697264386E-5 0.0 11.166616760149278 3.349430697264386E-5 82-83 4.3542599064437016E-4 3.349430697264386E-5 0.0 12.032880021382766 3.349430697264386E-5 84-85 4.3542599064437016E-4 3.349430697264386E-5 0.0 12.899494972839467 3.349430697264386E-5 86-87 4.3542599064437016E-4 3.349430697264386E-5 0.0 13.785251920731033 3.349430697264386E-5 88-89 4.3542599064437016E-4 3.349430697264386E-5 0.0 14.69823974019136 3.349430697264386E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 22290 0.0 28.070967 1 TACGGAC 70 0.008497011 27.074852 3 GGCGAAC 70 0.008523448 27.057615 8 CATTCCG 710 0.0 26.677414 9 ACATGGG 21485 0.0 26.640093 3 CATGGGT 2795 0.0 26.614725 4 TACATGG 23180 0.0 26.531721 2 CATGGGG 7785 0.0 26.353205 4 GTATGGT 655 0.0 26.052818 1 GAGTACT 20125 0.0 24.224274 12-13 ATGGGGA 4060 0.0 24.040602 5 GATTGGC 1400 0.0 24.014437 9 GCTCGCA 60 1.6984598E-4 23.777803 86-87 CATGGGA 9425 0.0 23.024397 4 AGTACTT 21530 0.0 22.687439 12-13 ATGGGTA 1730 0.0 22.184162 5 GTACTTT 21930 0.0 22.166029 14-15 GGATATG 1630 0.0 22.101385 1 GTATAGG 2030 0.0 21.949535 1 CATATAG 1280 0.0 21.469513 3 >>END_MODULE