##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938781_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2985582 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.929598986060338 31.0 31.0 34.0 30.0 34.0 2 32.148035123470066 33.0 31.0 34.0 30.0 34.0 3 32.213748274205834 34.0 31.0 34.0 30.0 34.0 4 35.87867022242229 37.0 35.0 37.0 35.0 37.0 5 35.80447229384421 37.0 35.0 37.0 35.0 37.0 6 35.85003359478989 37.0 35.0 37.0 35.0 37.0 7 35.7900539995217 37.0 35.0 37.0 33.0 37.0 8 35.768245856251816 37.0 35.0 37.0 33.0 37.0 9 37.375170737229794 39.0 37.0 39.0 34.0 39.0 10-11 37.301894237036535 39.0 37.0 39.0 34.0 39.0 12-13 37.258100263198266 39.0 37.0 39.0 33.0 39.0 14-15 38.368187844112136 40.0 38.0 41.0 33.0 41.0 16-17 38.33923134584815 40.0 38.0 41.0 33.0 41.0 18-19 38.4716222833605 40.0 38.0 41.0 33.5 41.0 20-21 38.57405105604201 40.0 38.0 41.0 34.0 41.0 22-23 38.51064248109749 40.0 38.0 41.0 34.0 41.0 24-25 38.429609369295505 40.0 38.0 41.0 34.0 41.0 26-27 38.24146481322569 40.0 38.0 41.0 33.5 41.0 28-29 38.0948639159802 40.0 37.0 41.0 33.0 41.0 30-31 37.82265183136822 40.0 37.0 41.0 33.0 41.0 32-33 37.52405762092617 40.0 36.0 41.0 33.0 41.0 34-35 37.393888863209924 40.0 36.0 41.0 32.0 41.0 36-37 37.23858329799684 40.0 36.0 41.0 32.0 41.0 38-39 37.100743674097714 40.0 36.0 41.0 31.0 41.0 40-41 36.941183494541434 40.0 35.5 41.0 31.0 41.0 42-43 36.67457852438821 40.0 35.0 41.0 30.5 41.0 44-45 36.264387814503166 40.0 35.0 41.0 30.0 41.0 46-47 35.76397583452741 39.0 35.0 41.0 28.0 41.0 48-49 35.32043032145826 39.0 35.0 41.0 25.5 41.0 50-51 35.05687098863806 39.0 35.0 41.0 24.0 41.0 52-53 34.90087694794516 39.0 35.0 41.0 23.5 41.0 54-55 34.70918802431151 39.0 35.0 40.5 23.0 41.0 56-57 34.48985859373482 39.0 34.5 40.0 22.5 41.0 58-59 34.249745443267 38.5 34.0 40.0 21.0 41.0 60-61 33.92146104176673 38.0 34.0 40.0 19.0 41.0 62-63 33.60971026754582 38.0 33.5 40.0 17.5 41.0 64-65 33.28833339697252 37.5 33.0 40.0 13.5 41.0 66-67 32.90931617352999 37.0 33.0 39.5 10.0 41.0 68-69 32.455300172629656 36.5 32.0 39.0 8.0 40.5 70-71 31.970956249066347 36.0 31.5 39.0 4.5 40.0 72-73 31.611583604134804 35.5 31.0 38.5 2.0 40.0 74-75 31.172202605723108 35.0 31.0 37.5 2.0 40.0 76-77 29.087567348677744 33.0 28.5 35.5 2.0 38.5 78-79 30.115656679334215 34.5 30.0 36.0 2.0 39.0 80-81 30.108211229837266 35.0 30.0 36.0 2.0 38.5 82-83 29.75835783441888 34.0 30.0 36.0 2.0 37.5 84-85 29.322466775322198 34.0 29.5 35.0 2.0 37.0 86-87 28.816020461002243 34.0 29.0 35.0 2.0 36.5 88-89 28.28071796386768 34.0 29.0 35.0 2.0 36.0 90-91 27.847257084213396 34.0 27.5 35.0 2.0 36.0 92-93 27.569087032277125 34.0 27.0 35.0 2.0 35.0 94-95 27.187562927429227 33.5 26.5 35.0 2.0 35.0 96-97 26.692524271649546 33.0 25.5 35.0 2.0 35.0 98-99 26.044050372758143 33.0 24.5 35.0 2.0 35.0 100-101 23.10973605816219 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.043632536648395615 1101 2 0.0949200320815109 1101 3 0.11071477914610739 1101 4 0.1399561570600767 1101 5 0.10229137740099503 1101 6 0.14954305445531446 1101 7 0.11839782962782408 1101 8 0.13586463293125206 1101 9 0.18196828330221138 1101 10-11 0.16171338883519581 1101 12-13 0.2019760921456708 1101 14-15 0.2802801168687665 1101 16-17 0.18630829522094672 1101 18-19 0.15365901073171528 1101 20-21 0.2044789260194122 1101 22-23 0.14223886423724252 1101 24-25 0.16946990049045496 1101 26-27 0.18872697922197545 1101 28-29 0.28053622337528594 1101 30-31 0.23845833260287463 1101 32-33 0.405060123972504 1101 34-35 0.3602897025108902 1101 36-37 0.37646596699880064 1101 38-39 0.3569371197889666 1101 40-41 0.31104225870383573 1101 42-43 0.3872008006087029 1101 44-45 0.5181584909581147 1101 46-47 0.48180518609887457 1101 48-49 0.39930802720825653 1101 50-51 0.49878186557575077 1101 52-53 0.4767589280663671 1101 54-55 0.35142655808530776 1101 56-57 0.1978207301011352 1101 58-59 0.3495236646698032 1101 60-61 0.5540284401959497 1101 62-63 0.3919429775145957 1101 64-65 0.3865321200968239 1101 66-67 0.40172413302093446 1101 68-69 0.45307781671753133 1101 70-71 0.36776887530531255 1101 72-73 0.40825221172511306 1101 74-75 0.4422862485062886 1101 76-77 0.3639212382993371 1101 78-79 0.4230128685985477 1101 80-81 0.571445242584911 1101 82-83 0.6026550040759098 1101 84-85 0.5473874172659166 1101 86-87 0.6273411298371201 1101 88-89 0.7118435392764404 1101 90-91 0.7582635821289543 1101 92-93 0.6773893251996519 1101 94-95 0.7098156712949901 1101 96-97 0.6458823174215382 1101 98-99 0.6521232857442136 1101 100-101 1.0751506989264143 1102 1 0.03412068460359308 1102 2 0.08306355904166196 1102 3 0.12916802217814194 1102 4 0.06282174705747678 1102 5 0.09344975197770822 1102 6 0.08482698256159438 1102 7 0.06682502420974856 1102 8 0.11837778739855764 1102 9 0.17206492305214738 1102 10-11 0.19571729610270694 1102 12-13 0.233118837652313 1102 14-15 0.18383260449574834 1102 16-17 0.2587996992324051 1102 18-19 0.14551600474057125 1102 20-21 0.1901396449566377 1102 22-23 0.16223769205699057 1102 24-25 0.10047159363971758 1102 26-27 0.2206695423894658 1102 28-29 0.20127534814736947 1102 30-31 0.1338240528425132 1102 32-33 0.2775162323340581 1102 34-35 0.22950551392010965 1102 36-37 0.156907878955046 1102 38-39 0.12556592937479394 1102 40-41 0.0820016231216556 1102 42-43 0.11096532261964143 1102 44-45 -0.12263260047194535 1102 46-47 -0.05861705927753036 1102 48-49 -0.02411539034840615 1102 50-51 0.029679495166789138 1102 52-53 0.01277025914216523 1102 54-55 0.1553876156523799 1102 56-57 0.016789862687744517 1102 58-59 -0.01098753616374637 1102 60-61 0.0015791044314283909 1102 62-63 -0.19652680628326635 1102 64-65 -0.04689911199486829 1102 66-67 -0.14100730745835932 1102 68-69 -0.13217615567111096 1102 70-71 -0.18415610515834757 1102 72-73 -0.1093868869985144 1102 74-75 0.0049920312622155905 1102 76-77 -0.10281007193770009 1102 78-79 -0.0737091944387025 1102 80-81 -0.009879972314802643 1102 82-83 -0.015667605978791244 1102 84-85 0.01786670874807328 1102 86-87 -0.051443709964907214 1102 88-89 0.1594637528737337 1102 90-91 0.17481020885466592 1102 92-93 0.3195921123838126 1102 94-95 0.3790400786927499 1102 96-97 0.4173046930401867 1102 98-99 0.40561208491066125 1102 100-101 0.6228564816823408 1103 1 0.043157728129504136 1103 2 0.07229119841962017 1103 3 -0.058043776908576206 1103 4 0.02262984739391527 1103 5 -0.0036555572853629315 1103 6 0.022621295091326488 1103 7 0.09120731804200943 1103 8 0.04050917289182365 1103 9 -0.03345129463140495 1103 10-11 -0.08449764435486884 1103 12-13 0.11778690557682125 1103 14-15 0.10051752112821077 1103 16-17 0.03330225185088409 1103 18-19 -0.0052826562042724845 1103 20-21 0.0983456487030594 1103 22-23 0.02572805403784173 1103 24-25 0.040438649480535105 1103 26-27 0.10492870497578366 1103 28-29 0.10867538247428143 1103 30-31 -0.029301483833805264 1103 32-33 -0.03340550638008466 1103 34-35 -0.040074386297568765 1103 36-37 -0.09465357538557839 1103 38-39 0.029979229474513147 1103 40-41 0.06682889865471253 1103 42-43 0.05832622477571903 1103 44-45 0.0040692300590379205 1103 46-47 -0.17258100787068287 1103 48-49 -0.13234759765035875 1103 50-51 -0.1425621540732891 1103 52-53 -0.3062785925360245 1103 54-55 -0.13977894881448094 1103 56-57 -0.19914239564621283 1103 58-59 -0.3074514185477213 1103 60-61 -0.4118115236084634 1103 62-63 -0.3679260073931303 1103 64-65 -0.2063260384527652 1103 66-67 -0.40620664292193 1103 68-69 -0.3315093273861436 1103 70-71 -0.40043653464200446 1103 72-73 -0.40124744894127673 1103 74-75 -0.35913151964805934 1103 76-77 -0.4479062509550964 1103 78-79 -0.3991599006869144 1103 80-81 -0.3605988384673431 1103 82-83 -0.3431448860446302 1103 84-85 -0.3309925071111657 1103 86-87 -0.31586928932068403 1103 88-89 -0.12831520421966403 1103 90-91 -0.14770965899181476 1103 92-93 -0.23487684162321543 1103 94-95 -0.15431094861904526 1103 96-97 -0.13719023533676022 1103 98-99 0.10994806032974225 1103 100-101 0.5068221593536535 1104 1 -0.009974282334525242 1104 2 0.0392275271947895 1104 3 -0.013356871445111551 1104 4 0.019823750869157664 1104 5 0.041263915613576785 1104 6 0.05210786363274167 1104 7 0.047982730451359146 1104 8 0.04125073220384934 1104 9 -0.01650231624275733 1104 10-11 0.0943441447553397 1104 12-13 0.07400050974428751 1104 14-15 0.05552906742472885 1104 16-17 0.062270931808896535 1104 18-19 0.09639724253867854 1104 20-21 0.11212727414643808 1104 22-23 0.08640184049257016 1104 24-25 0.0287615337778675 1104 26-27 0.09530146571539433 1104 28-29 0.13343429111650096 1104 30-31 0.04015961757098552 1104 32-33 0.07492079625756531 1104 34-35 0.11543008768087759 1104 36-37 0.09660093671713099 1104 38-39 0.18053945259890725 1104 40-41 0.22968211514630354 1104 42-43 0.3206633570864099 1104 44-45 0.21621546535487823 1104 46-47 0.19241729235708505 1104 48-49 0.14284377239362556 1104 50-51 0.15699021626629417 1104 52-53 0.10556111988230299 1104 54-55 0.08445991598577507 1104 56-57 0.17491657398231553 1104 58-59 0.24699154046690097 1104 60-61 0.2176515375779502 1104 62-63 0.17410165986150616 1104 64-65 0.2792744535476501 1104 66-67 0.22723449928491846 1104 68-69 0.3051135779249492 1104 70-71 0.3508681799485025 1104 72-73 0.20731806233554906 1104 74-75 0.07880273519196734 1104 76-77 0.16156208810867767 1104 78-79 0.2552906267938262 1104 80-81 0.3639514110727511 1104 82-83 0.30910182690412213 1104 84-85 0.37115163683204244 1104 86-87 0.45948648184148055 1104 88-89 0.646807874201297 1104 90-91 0.5013510304578546 1104 92-93 0.6824090964048288 1104 94-95 0.691908470987368 1104 96-97 0.8143834229427327 1104 98-99 0.8998442457852285 1104 100-101 1.3020161457812875 1105 1 -0.029155342139482343 1105 2 0.03165832409253255 1105 3 0.032172079421584954 1105 4 0.005319793423716135 1105 5 0.02693049585829499 1105 6 -0.04553959017278686 1105 7 -0.013133850812948822 1105 8 0.06997207094777735 1105 9 0.08472861388072772 1105 10-11 0.10171889847706694 1105 12-13 0.02933559627789606 1105 14-15 0.17902942816353118 1105 16-17 0.0690397001796228 1105 18-19 -0.027710135273800063 1105 20-21 0.030651102327958313 1105 22-23 0.06409423613807519 1105 24-25 0.05490653960892189 1105 26-27 -0.06494381967609542 1105 28-29 0.09099765588217679 1105 30-31 0.0374679744761508 1105 32-33 0.05644579890363133 1105 34-35 0.0036960385990383315 1105 36-37 -0.0775133718441694 1105 38-39 -0.07544029067935298 1105 40-41 0.03868275457161019 1105 42-43 -0.13836669250424194 1105 44-45 -0.07636354760938246 1105 46-47 0.12839058554791194 1105 48-49 0.029797007924507568 1105 50-51 0.03131492342699005 1105 52-53 0.08373551208840269 1105 54-55 0.10042242585389971 1105 56-57 0.03651219015623042 1105 58-59 0.11300851315465366 1105 60-61 0.02523780658713548 1105 62-63 -0.1593752043035792 1105 64-65 -0.11215245290593145 1105 66-67 -0.09963812593223054 1105 68-69 -0.021247252152996055 1105 70-71 0.007493393211653654 1105 72-73 -0.21872574970739578 1105 74-75 -0.09526884791244328 1105 76-77 0.020744929759395347 1105 78-79 -0.19123506528574907 1105 80-81 -0.06172280149222331 1105 82-83 -0.0031673099736728716 1105 84-85 0.041919097706689 1105 86-87 0.0654334163385002 1105 88-89 0.08997852550233532 1105 90-91 0.22319471160554016 1105 92-93 0.32371990371204973 1105 94-95 0.3152702167495356 1105 96-97 0.4032197829862767 1105 98-99 0.6384235612263076 1105 100-101 1.0697719110476243 1106 1 -0.035477181219942366 1106 2 -0.050822914626948545 1106 3 -0.024357321167379098 1106 4 0.005868382765406466 1106 5 0.018097020034225864 1106 6 -0.09026885777038984 1106 7 -0.09272442742546616 1106 8 -0.043671417225489506 1106 9 -0.07666494553791381 1106 10-11 -0.0961204669641802 1106 12-13 0.023220606252252196 1106 14-15 0.03914532063051723 1106 16-17 -0.06879515305491424 1106 18-19 -0.1044632463215791 1106 20-21 -0.036746779309737576 1106 22-23 -0.08246959971259571 1106 24-25 -0.12523856345937645 1106 26-27 -0.13878085776861582 1106 28-29 -0.023006096373926255 1106 30-31 0.046809429781554 1106 32-33 0.014194920210748307 1106 34-35 -0.008465783382533232 1106 36-37 -0.051546572186147444 1106 38-39 -0.11001899306370433 1106 40-41 -0.12354081339647394 1106 42-43 -0.16172709907781524 1106 44-45 0.02479798939071287 1106 46-47 -0.055533371894860295 1106 48-49 -0.032635546459459874 1106 50-51 -0.046826284894471826 1106 52-53 0.03907867728266723 1106 54-55 0.14982467720756176 1106 56-57 0.1160495702265294 1106 58-59 0.1743669248578925 1106 60-61 0.14688737436522814 1106 62-63 0.12572667657416048 1106 64-65 0.029980395958894235 1106 66-67 0.09181504211183977 1106 68-69 0.07469223678022985 1106 70-71 0.01866229223321625 1106 72-73 0.20439954714830577 1106 74-75 0.2857031764059741 1106 76-77 0.10831308782912075 1106 78-79 0.22470252377096145 1106 80-81 0.1155831736193953 1106 82-83 0.17184622664644422 1106 84-85 0.04950340472673176 1106 86-87 0.026839562439000986 1106 88-89 0.25895639194101605 1106 90-91 0.4136554316587393 1106 92-93 0.5555053126604648 1106 94-95 0.4642670819532988 1106 96-97 0.4351080227506898 1106 98-99 0.43475964938057743 1106 100-101 0.9552917647571348 1107 1 -0.12145652657264705 1107 2 -0.07705781748008889 1107 3 -0.15888559002787161 1107 4 -0.08022473818680709 1107 5 -0.10069462475252067 1107 6 -0.08814037886263293 1107 7 -0.08678475766643601 1107 8 0.004394397582842657 1107 9 -0.1683925842566225 1107 10-11 -0.07798142734693414 1107 12-13 -0.06479240500861039 1107 14-15 -0.14154083375422033 1107 16-17 -0.11460879445755978 1107 18-19 -0.12277907571652946 1107 20-21 -0.11163767142712544 1107 22-23 -0.1539860242414477 1107 24-25 -0.11717799983256327 1107 26-27 -0.09408951054882664 1107 28-29 -0.17409075708925315 1107 30-31 -0.277007595715844 1107 32-33 -0.17148102354327222 1107 34-35 -0.16688488601395335 1107 36-37 -0.13309625419793036 1107 38-39 -0.0642143953625478 1107 40-41 -0.18370593388192447 1107 42-43 -0.24234265424439627 1107 44-45 -0.25794487775439734 1107 46-47 -0.15305160516400917 1107 48-49 -0.3173878657312983 1107 50-51 -0.20201124317675578 1107 52-53 -0.18020307777889144 1107 54-55 -0.28609136223464304 1107 56-57 -0.3516501205372293 1107 58-59 -0.2647756738934959 1107 60-61 -0.2975177834619487 1107 62-63 -0.3327735276384587 1107 64-65 -0.3516573892094428 1107 66-67 -0.2816959561961099 1107 68-69 -0.14882342924462932 1107 70-71 -0.25142201840334266 1107 72-73 -0.23833077550762383 1107 74-75 -0.12168698638064868 1107 76-77 -0.19624581292354648 1107 78-79 -0.19949812970940428 1107 80-81 -0.19840247315113047 1107 82-83 -0.07922501438278928 1107 84-85 -0.15759181669809053 1107 86-87 -0.1266536401982563 1107 88-89 -0.11224875421686775 1107 90-91 0.1613384936810256 1107 92-93 0.14304630104951954 1107 94-95 0.19643465299011353 1107 96-97 0.08779925327755933 1107 98-99 0.008422931952488 1107 100-101 0.3265074132780512 1108 1 -0.19096601416448777 1108 2 -0.18216965920509764 1108 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-1.0797417934343585 2316 54-55 -1.0379218236916472 2316 56-57 -0.9429976578601114 2316 58-59 -0.8824692233945086 2316 60-61 -0.8355230955698687 2316 62-63 -0.8950869132295836 2316 64-65 -0.901806882170753 2316 66-67 -0.985242781017412 2316 68-69 -1.0435048466508476 2316 70-71 -1.1551511411861277 2316 72-73 -1.3176158567143865 2316 74-75 -1.2359275218503392 2316 76-77 -1.253584636777962 2316 78-79 -1.266678741378776 2316 80-81 -1.2580728980046594 2316 82-83 -1.3742341940275722 2316 84-85 -1.4744189113261257 2316 86-87 -1.541182370472093 2316 88-89 -1.8031866606823286 2316 90-91 -2.4074543110889373 2316 92-93 -2.4392389988572454 2316 94-95 -2.2770432237781897 2316 96-97 -2.381712096101449 2316 98-99 -2.7950929583291746 2316 100-101 -3.573987107711396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 26.0 5 80.0 6 256.0 7 624.0 8 1333.0 9 2540.0 10 4618.0 11 7663.0 12 11369.0 13 16458.0 14 19268.0 15 26681.0 16 36289.0 17 50893.0 18 43944.0 19 23411.0 20 18316.0 21 18006.0 22 18826.0 23 20682.0 24 23954.0 25 27462.0 26 32809.0 27 39218.0 28 47440.0 29 58076.0 30 70644.0 31 85118.0 32 103724.0 33 129908.0 34 168438.0 35 230301.0 36 331544.0 37 577111.0 38 652357.0 39 86181.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.399220845509724 23.7054693807282 16.277476107038584 19.61783366672349 2 13.780127291764218 24.554073544119706 41.11074490668821 20.555054257427866 3 17.6960806971639 30.78434958410119 32.6240913831876 18.89547833554731 4 10.47169362623435 20.197301564653056 42.43999997320456 26.89100483590804 5 9.543197942645689 41.94254252604685 36.49636821229495 12.017891319012508 6 23.846539803629575 42.94964934810031 19.31090822492901 13.892902623341111 7 23.101793888092843 36.92405701802864 24.535182754987133 15.438966338891378 8 23.705528771274746 35.46206401297972 21.974844435691267 18.857562780054273 9 25.438658191267233 18.15702934972143 24.43630756080389 31.968004898207454 10-11 21.88283557443741 29.223431143408558 29.666795284805442 19.22693799734859 12-13 24.417885691968934 28.263651777107444 29.467085479481053 17.851377051442565 14-15 21.42501504316337 28.946211542799706 30.012203677314474 19.61656973672245 16-17 17.376171172717672 32.09094890425378 30.883756528379873 19.649123394648676 18-19 17.16167952681875 30.90355338110296 34.37845724397177 17.556309848106515 20-21 18.843886667285194 29.06208205371082 33.97268404374224 18.121347235261748 22-23 18.299145694206356 29.044387325486287 33.93803955141745 18.71842742888991 24-25 18.61230071724709 29.940879198762588 34.00710481239504 17.439715271595286 26-27 18.154360421057568 29.016781510444794 35.387642759231674 17.44121530926596 28-29 17.08809203699647 29.88594853532745 35.202483133941726 17.823476293734352 30-31 17.900044279473818 29.490698965896765 34.47296038092406 18.13629637370536 32-33 17.658650809121973 29.997082645862683 34.49148273267993 17.852783812335417 34-35 18.483625289559804 29.40656381888827 34.43810031686397 17.671710574687964 36-37 18.530710735529038 28.932594385598527 34.531585761135766 18.005109117736673 38-39 17.566410070882075 30.372968337554102 34.36103964840348 17.69958194316035 40-41 17.550603130833718 30.11353149508314 34.624342495106916 17.711522878976226 42-43 17.36026430550887 30.300458769757473 33.39015828084185 18.949118643891804 44-45 17.923598027147655 29.743596184925597 33.2344729988863 19.098332789040455 46-47 18.783442543386755 30.417285903244682 32.56542973427508 18.233841819093488 48-49 18.393150547054233 30.552440119286643 32.317707622451444 18.736701711207683 50-51 17.601894448789427 30.733496004943788 32.66852337832203 18.99608616794476 52-53 18.077446876354426 31.678563844503348 31.504560919780467 18.73942835936176 54-55 17.863903922250334 30.609944727694632 31.892860420514324 19.633290929540706 56-57 18.421600880498342 30.68078518694178 31.35606390981725 19.541550022742634 58-59 18.709527126277127 30.187741715176475 31.742858008929296 19.359873149617098 60-61 18.235383567778385 31.246422364172094 31.857413062402678 18.660781005646847 62-63 17.740795596972383 30.85642933270632 32.274377324086224 19.128397746235073 64-65 17.603368455463624 31.148064263517128 31.773151767394097 19.47541551362515 66-67 17.86030691213832 30.658924919905743 30.75719843445669 20.723569733499243 68-69 17.49307541620772 30.691476988308885 31.31621771939823 20.499229876085163 70-71 18.483904030440158 30.48488878021658 31.138256858916176 19.89295033042709 72-73 18.296064217964872 29.722680535989298 31.586956914933168 20.39429833111266 74-75 17.38433243501602 31.484179633987612 30.614282240447594 20.517205690548778 76-77 17.590499795066336 30.08322234506135 31.41687530226668 20.909402557605638 78-79 17.49485592743582 29.996217125598466 31.438735360627884 21.070191586337824 80-81 17.9895105861287 30.30274001898793 30.68444120517226 21.023308189711116 82-83 17.900479705464463 29.89577911442392 31.18999913584688 21.013742044264735 84-85 17.297196995426685 29.90215977990221 30.86426700053792 21.936376224133184 86-87 17.18168781395973 30.20472480564897 30.91076205731154 21.70282532307976 88-89 17.666407803922056 29.499078746584573 30.622628188304667 22.2118852611887 90-91 17.69034391061216 28.91781779658526 31.014514221242994 22.377324071559578 92-93 18.166591974362117 29.68871730871904 30.65603289408899 21.488657822829854 94-95 17.729611467648766 28.598432164722347 30.896694664004315 22.77526170362457 96-97 17.27440701250326 28.513105403419736 31.13480573214967 23.077681851927338 98-99 17.441915769743748 28.880471386110163 30.218286040425003 23.45932680372109 100-101 17.298356992203477 28.06132582787623 31.04635946142115 23.593957718499137 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4444.0 1 2702.5 2 1182.0 3 1373.0 4 1131.0 5 1196.5 6 2285.5 7 4401.5 8 6349.5 9 7096.0 10 6571.5 11 5987.5 12 6384.5 13 6947.0 14 7568.0 15 8169.0 16 8640.0 17 9427.0 18 10418.0 19 11425.5 20 12557.5 21 14010.0 22 15788.0 23 17819.0 24 20097.0 25 23849.0 26 31215.5 27 39090.0 28 41978.5 29 47246.5 30 59151.0 31 70075.0 32 85504.5 33 105940.0 34 125233.0 35 138569.5 36 154965.5 37 168518.0 38 162127.5 39 151900.0 40 140097.0 41 125728.5 42 111897.0 43 105070.0 44 103023.0 45 98077.0 46 90446.0 47 96007.5 48 130465.0 49 119503.5 50 72815.0 51 50104.0 52 34949.5 53 25423.5 54 19274.5 55 15373.0 56 12096.0 57 9331.5 58 7024.0 59 5334.5 60 4056.0 61 3075.5 62 2357.0 63 1867.0 64 1307.5 65 867.5 66 583.0 67 498.5 68 386.0 69 261.0 70 230.0 71 194.5 72 151.5 73 137.0 74 124.5 75 103.0 76 72.5 77 43.5 78 35.5 79 26.0 80 15.0 81 13.5 82 9.5 83 5.0 84 2.5 85 2.0 86 2.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38816552350596967 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.177129953221851E-4 16-17 0.00199291126487231 18-19 1.0048292091793158E-4 20-21 3.349430697264386E-5 22-23 0.0 24-25 0.0 26-27 1.5072438137689738E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007753932064167054 36-37 0.01763475262109699 38-39 0.0114550529846442 40-41 0.008139116594352458 42-43 0.0032656949298327763 44-45 0.0019091754974407001 46-47 0.0017082096556048367 48-49 1.674715348632193E-5 50-51 5.694032185349457E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.861503720212675E-4 60-61 7.033804464255211E-4 62-63 0.0 64-65 0.0 66-67 0.0012392893579878227 68-69 7.871162138571306E-4 70-71 0.0017417039625774809 72-73 0.0 74-75 0.0 76-77 0.09745168613690731 78-79 0.03614035722348272 80-81 1.674715348632193E-5 82-83 0.0 84-85 0.0 86-87 0.15095884152570588 88-89 0.3264020214484144 90-91 0.2813019371097495 92-93 0.0 94-95 1.674715348632193E-5 96-97 1.674715348632193E-5 98-99 1.3397722789057544E-4 100-101 7.033804464255211E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2985582.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.095301376697634 #Duplication Level Percentage of deduplicated Percentage of total 1 71.16957499158157 17.148523582736406 2 12.647669418603646 6.0949881270819395 3 5.256615988020534 3.7997923935876585 4 2.852695282639106 2.749462102842916 5 1.70632394993995 2.055719491004011 6 1.1056338602824864 1.5984348645472868 7 0.7950428555553163 1.3409758048396943 8 0.5803584948712524 1.1187130272359564 9 0.4251606939454716 0.9219937548727839 >10 2.6287197936671376 11.950465244696442 >50 0.3434874472775502 5.891115777993843 >100 0.40794072253499186 20.908028562387287 >500 0.05385100072068167 8.895755472583659 >1k 0.024693334527359213 9.857392444556922 >5k 0.0013951036456135149 2.0881913062879414 >10k+ 8.37062187368109E-4 3.5804480427453518 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26128 0.8751392525812387 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24152 0.8089545020032946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19521 0.6538423664129809 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13653 0.4572977730975066 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12858 0.43066979905425473 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10200 0.3416419311209674 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8823 0.29552027041963674 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6886 0.23064179781362562 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6690 0.22407691364698742 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6658 0.2230050958238628 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6331 0.2120524574438083 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5612 0.18797005073047734 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5528 0.18515652894477524 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5332 0.17859164477813705 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5226 0.1750412482390368 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5034 0.16861034130028918 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4656 0.1559494932646298 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4451 0.14908316033523783 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4184 0.14014018037354192 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3607 0.12081396525032641 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3594 0.12037853925968203 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3500 0.11723007440425351 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3351 0.11223942266532959 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3214 0.10765070261007736 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3191 0.10688033354970655 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3068 0.10276053379207135 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2999 0.10044942661095893 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.349430697264386E-5 0.0 14-15 0.0 0.0 0.0 3.349430697264386E-5 0.0 16-17 0.0 0.0 0.0 6.698861394528772E-5 0.0 18-19 0.0 0.0 0.0 1.5072438137689738E-4 0.0 20-21 0.0 0.0 0.0 3.5169022321276055E-4 0.0 22-23 1.674715348632193E-5 0.0 0.0 0.0010048292091793158 0.0 24-25 3.349430697264386E-5 0.0 0.0 0.0024785787159756455 0.0 26-27 3.349430697264386E-5 0.0 0.0 0.005275353348191408 0.0 28-29 3.349430697264386E-5 0.0 0.0 0.01379965447272927 0.0 30-31 3.349430697264386E-5 0.0 0.0 0.04149944633910574 0.0 32-33 3.349430697264386E-5 0.0 0.0 0.09788711212755169 0.0 34-35 6.698861394528772E-5 0.0 0.0 0.18008214143841972 0.0 36-37 6.698861394528772E-5 0.0 0.0 0.30226937327462455 3.349430697264386E-5 38-39 6.698861394528772E-5 0.0 0.0 0.4915959434374939 3.349430697264386E-5 40-41 6.698861394528772E-5 0.0 0.0 0.7191060235491773 3.349430697264386E-5 42-43 6.698861394528772E-5 0.0 0.0 0.938359756992104 3.349430697264386E-5 44-45 6.698861394528772E-5 0.0 0.0 1.1907896014914345 3.349430697264386E-5 46-47 6.698861394528772E-5 0.0 0.0 1.4978821549701198 3.349430697264386E-5 48-49 6.698861394528772E-5 0.0 0.0 1.8266287779066193 3.349430697264386E-5 50-51 6.698861394528772E-5 0.0 0.0 2.198851011293611 3.349430697264386E-5 52-53 6.698861394528772E-5 0.0 0.0 2.5539241595106077 3.349430697264386E-5 54-55 6.698861394528772E-5 0.0 0.0 2.9237682971025416 3.349430697264386E-5 56-57 6.698861394528772E-5 0.0 0.0 3.3647041012439116 3.349430697264386E-5 58-59 6.698861394528772E-5 0.0 0.0 3.8357177930467157 3.349430697264386E-5 60-61 1.3397722789057544E-4 0.0 0.0 4.365932672423668 3.349430697264386E-5 62-63 1.8421868834954123E-4 0.0 0.0 4.897989738684116 3.349430697264386E-5 64-65 2.1771299532218508E-4 0.0 0.0 5.422795287484986 3.349430697264386E-5 66-67 2.3446014880850702E-4 0.0 0.0 6.002682224102369 3.349430697264386E-5 68-69 2.3446014880850702E-4 0.0 0.0 6.642071797056655 3.349430697264386E-5 70-71 2.3446014880850702E-4 0.0 0.0 7.361378786447668 3.349430697264386E-5 72-73 2.3446014880850702E-4 0.0 0.0 8.074020408751124 3.349430697264386E-5 74-75 2.3446014880850702E-4 0.0 0.0 8.794114514355995 3.349430697264386E-5 76-77 2.3446014880850702E-4 0.0 0.0 9.510607981961307 3.349430697264386E-5 78-79 2.3446014880850702E-4 0.0 0.0 10.346726366919416 3.349430697264386E-5 80-81 2.3446014880850702E-4 0.0 0.0 11.255812099617428 3.349430697264386E-5 82-83 2.3446014880850702E-4 0.0 0.0 12.12611142484112 3.349430697264386E-5 84-85 2.3446014880850702E-4 0.0 0.0 12.993630722586083 3.349430697264386E-5 86-87 2.3446014880850702E-4 0.0 0.0 13.88119636305417 3.349430697264386E-5 88-89 2.3446014880850702E-4 0.0 0.0 14.796746496997905 3.349430697264386E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGAGT 50 0.0016239809 37.96301 3 CATTCCG 635 0.0 34.375954 9 TCACGCA 120 5.4263983E-6 27.68136 4 GTACCCG 70 0.008279762 27.219 1 GTACATG 22400 0.0 25.2839 1 CATGGGG 7575 0.0 24.932802 4 CTTTGCG 345 0.0 24.758486 2 ACTCCCG 745 0.0 24.204603 6 ACATGGG 21380 0.0 24.082006 3 ATGGGCG 375 0.0 24.043238 5 AGGCTTA 1920 0.0 23.974035 6 CCTTTAG 1055 0.0 23.477999 1 TACATGG 23570 0.0 23.414818 2 CTCCCGC 775 0.0 23.26765 7 ATGGGGA 4040 0.0 23.139582 5 CATGGGT 2570 0.0 23.080622 4 GATTGGC 1325 0.0 22.921062 9 GAGTACT 21805 0.0 22.372707 12-13 CTTACTA 2235 0.0 22.293713 9 TACCTGG 1100 0.0 22.001291 2 >>END_MODULE