##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938780_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2982961 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67295985431925 33.0 31.0 34.0 30.0 34.0 2 31.730017254667427 33.0 31.0 34.0 30.0 34.0 3 31.718466651089305 33.0 31.0 34.0 30.0 34.0 4 35.30112428556726 37.0 35.0 37.0 33.0 37.0 5 35.361496177791125 37.0 35.0 37.0 33.0 37.0 6 35.45206692276567 37.0 35.0 37.0 33.0 37.0 7 35.389302441433195 37.0 35.0 37.0 33.0 37.0 8 35.366520045015676 37.0 35.0 37.0 33.0 37.0 9 36.919938276095465 39.0 37.0 39.0 33.0 39.0 10-11 36.815185817045545 39.0 37.0 39.0 33.0 39.0 12-13 36.74562389518334 39.0 37.0 39.0 32.0 39.0 14-15 37.841206271218425 40.0 37.5 41.0 32.5 41.0 16-17 37.77276286884073 40.0 37.5 41.0 32.5 41.0 18-19 37.85353294930775 40.0 38.0 41.0 33.0 41.0 20-21 37.80511210170029 40.0 38.0 41.0 32.0 41.0 22-23 37.79976255137093 40.0 37.5 41.0 32.0 41.0 24-25 37.73123936249921 40.0 37.0 41.0 32.0 41.0 26-27 37.58789320410156 40.0 37.0 41.0 32.0 41.0 28-29 37.44651891191336 40.0 37.0 41.0 32.0 41.0 30-31 37.18827969926526 39.5 36.5 41.0 31.5 41.0 32-33 36.9340981997418 39.0 35.5 41.0 31.0 41.0 34-35 36.61860731668969 39.0 35.0 41.0 30.0 41.0 36-37 36.37076532344875 39.0 35.0 41.0 30.0 41.0 38-39 36.19040108134166 39.0 35.0 41.0 30.0 41.0 40-41 36.06686778673942 39.0 35.0 41.0 30.0 41.0 42-43 35.923311099273505 39.0 35.0 41.0 29.5 41.0 44-45 35.58373274072306 39.0 35.0 41.0 26.5 41.0 46-47 35.07459132050336 39.0 35.0 41.0 23.5 41.0 48-49 34.635352088076246 39.0 35.0 41.0 21.0 41.0 50-51 33.49616907495606 38.0 34.0 40.0 16.5 40.5 52-53 33.609039474535535 38.0 33.5 39.5 15.0 40.5 54-55 34.156551661252024 39.0 34.0 40.5 14.5 41.0 56-57 34.096675249860795 39.0 34.0 41.0 12.5 41.0 58-59 33.94651002812306 39.0 34.0 41.0 8.5 41.0 60-61 33.66711331458909 38.5 34.0 40.0 8.0 41.0 62-63 33.33404945622822 38.0 33.5 40.0 4.5 41.0 64-65 32.99694431137383 38.0 33.0 40.0 2.0 41.0 66-67 32.61409217217389 37.0 32.5 40.0 2.0 41.0 68-69 32.191074405599 37.0 32.0 39.0 2.0 41.0 70-71 31.74700205601079 36.0 31.5 39.0 2.0 41.0 72-73 31.296170315334326 36.0 31.0 39.0 2.0 40.0 74-75 30.807614145810152 35.0 31.0 38.0 2.0 40.0 76-77 30.32905592798565 35.0 30.5 37.0 2.0 39.0 78-79 29.845390033594136 35.0 30.0 37.0 2.0 39.0 80-81 29.308437656409186 34.5 29.0 36.0 2.0 39.0 82-83 28.83432368039676 34.0 29.0 36.0 2.0 37.5 84-85 28.39554187936081 34.0 29.0 35.5 2.0 37.0 86-87 27.97236068456812 34.0 27.5 35.0 2.0 37.0 88-89 27.45869909127206 34.0 26.5 35.0 2.0 36.0 90-91 26.948831882146635 34.0 25.5 35.0 2.0 36.0 92-93 26.456568490167992 34.0 25.0 35.0 2.0 35.5 94-95 25.91139709838647 33.5 22.5 35.0 2.0 35.0 96-97 25.30393323948922 33.0 19.0 35.0 2.0 35.0 98-99 24.59513718080793 33.0 2.0 35.0 2.0 35.0 100-101 22.262960528146362 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29240083309129616 1101 2 0.31309772054083496 1101 3 0.4248770643648676 1101 4 0.2502852122035222 1101 5 0.2716270088943773 1101 6 0.3367048903936194 1101 7 0.2745976616592216 1101 8 0.35182782426436177 1101 9 0.5096103778240249 1101 10-11 0.43446733105574964 1101 12-13 0.41593497409477465 1101 14-15 0.5155121259954214 1101 16-17 0.634437230468869 1101 18-19 0.6396713498886726 1101 20-21 0.6318857424770457 1101 22-23 0.5475769825198427 1101 24-25 0.6072008391261292 1101 26-27 0.5423986280880158 1101 28-29 0.632703181818492 1101 30-31 0.5193355411583767 1101 32-33 0.3857968984841662 1101 34-35 0.4203366693192194 1101 36-37 0.4987267450068984 1101 38-39 0.5337815882334453 1101 40-41 0.6208283566363448 1101 42-43 0.7761545761939814 1101 44-45 0.5745775242606967 1101 46-47 0.6418995610162952 1101 48-49 0.6415847791509535 1101 50-51 0.6604469408202931 1101 52-53 0.6684621335679921 1101 54-55 0.6434983808776664 1101 56-57 0.6511427997302945 1101 58-59 0.6023840613385119 1101 60-61 0.4808290968096145 1101 62-63 0.35273847012880566 1101 64-65 0.34125054143830624 1101 66-67 0.24767698079924827 1101 68-69 0.23936667970292547 1101 70-71 0.188676521299584 1101 72-73 0.257038507499896 1101 74-75 0.24698449798248134 1101 76-77 0.27766724271232235 1101 78-79 0.19695264142522362 1101 80-81 0.10649302881428468 1101 82-83 0.28579027138802715 1101 84-85 0.26017897801241574 1101 86-87 0.04207736724167788 1101 88-89 0.020727362717522624 1101 90-91 -0.050094056681970756 1101 92-93 0.013525013736909841 1101 94-95 -0.04520690013370654 1101 96-97 0.021975718153363744 1101 98-99 0.09357475658203995 1101 100-101 0.47607519391926445 1102 1 0.10478023802410874 1102 2 0.09724341021081528 1102 3 0.2355863419731854 1102 4 0.18586345489300982 1102 5 0.21181098428532863 1102 6 0.259268885595084 1102 7 0.21609590701732628 1102 8 0.18633286887536116 1102 9 0.36211254420992844 1102 10-11 0.3961580854111233 1102 12-13 0.33538991613071545 1102 14-15 0.406354157441811 1102 16-17 0.5611846967122034 1102 18-19 0.4636588740948113 1102 20-21 0.5121833059432603 1102 22-23 0.4884137987079953 1102 24-25 0.3656948019979751 1102 26-27 0.42145156208773926 1102 28-29 0.5083488345211222 1102 30-31 0.5540074242449933 1102 32-33 0.47276637042868686 1102 34-35 0.5465883624111072 1102 36-37 0.6100440047235267 1102 38-39 0.5602678185825667 1102 40-41 0.6120478035003245 1102 42-43 0.6522843517337478 1102 44-45 0.705549897332638 1102 46-47 0.7640952394964913 1102 48-49 0.7263688214067443 1102 50-51 0.7197742994674954 1102 52-53 0.6520392356220057 1102 54-55 0.47815241508528317 1102 56-57 0.6290343367975808 1102 58-59 0.598970058706243 1102 60-61 0.5450477204345106 1102 62-63 0.4347855598031174 1102 64-65 0.36646096279383045 1102 66-67 0.49804771222240873 1102 68-69 0.5573252907166051 1102 70-71 0.44605405795015685 1102 72-73 0.33676966156061283 1102 74-75 0.3474080914621922 1102 76-77 0.25814848533285684 1102 78-79 0.2745456018472616 1102 80-81 0.3915045882661161 1102 82-83 0.4539742587280209 1102 84-85 0.28332775426887835 1102 86-87 0.18904944308123817 1102 88-89 0.3220748161654292 1102 90-91 0.2694392760373141 1102 92-93 0.18168242690908443 1102 94-95 0.138674578828887 1102 96-97 0.20640143856224924 1102 98-99 0.08727001970862247 1102 100-101 0.41347890145754107 1103 1 0.1912175345222593 1103 2 0.22925539130998374 1103 3 0.2968133684295857 1103 4 0.2890047475070858 1103 5 0.2868589879787038 1103 6 0.23457586842369693 1103 7 0.10518815924310587 1103 8 0.19556838056529813 1103 9 0.25093054173957086 1103 10-11 0.2541832399788788 1103 12-13 0.3676451264834597 1103 14-15 0.5120728969064174 1103 16-17 0.46949758155052734 1103 18-19 0.41164668380802993 1103 20-21 0.44965852572403975 1103 22-23 0.4252300774937936 1103 24-25 0.3850900819469487 1103 26-27 0.4443957951013431 1103 28-29 0.43711771431170376 1103 30-31 0.5370883419072783 1103 32-33 0.3361782885769813 1103 34-35 0.3892642539273794 1103 36-37 0.44818271411376287 1103 38-39 0.5128122697350648 1103 40-41 0.450902311566459 1103 42-43 0.3287523501633842 1103 44-45 0.28030029515397104 1103 46-47 0.20735024017513837 1103 48-49 0.18854684882588657 1103 50-51 0.17060110136342388 1103 52-53 0.24937636238629324 1103 54-55 0.25833794504264773 1103 56-57 0.23173834215668165 1103 58-59 0.23172988553780982 1103 60-61 0.24302555724968045 1103 62-63 0.0903711002685128 1103 64-65 0.08544257422929036 1103 66-67 4.904033286763365E-4 1103 68-69 0.03756362838531402 1103 70-71 -0.03390783584966783 1103 72-73 0.010203223330069733 1103 74-75 -0.025513653882534015 1103 76-77 -0.005375737377036671 1103 78-79 -0.040933953468513806 1103 80-81 -0.006670663281994393 1103 82-83 0.059363466884779115 1103 84-85 0.0881936528440157 1103 86-87 0.07520315657496113 1103 88-89 0.1529337356578715 1103 90-91 0.15742295551129004 1103 92-93 -0.09506251403433552 1103 94-95 -0.11906536301695425 1103 96-97 -0.19973182076082807 1103 98-99 -0.00973648116145398 1103 100-101 0.52915284315063 1104 1 0.14347542307965355 1104 2 0.24617500402480275 1104 3 0.1565156204975544 1104 4 0.3252661683288167 1104 5 0.32852607040153714 1104 6 0.31411750557168006 1104 7 0.3644412283550835 1104 8 0.3658795657364422 1104 9 0.47723992419199845 1104 10-11 0.34017200791043933 1104 12-13 0.38122995903351153 1104 14-15 0.4757969246190612 1104 16-17 0.45837402547167727 1104 18-19 0.5199337019050603 1104 20-21 0.4634030086397871 1104 22-23 0.425678678391165 1104 24-25 0.3747955436524819 1104 26-27 0.4381461484940772 1104 28-29 0.4892132085127372 1104 30-31 0.5411082882248408 1104 32-33 0.5203017249180562 1104 34-35 0.6394683902022678 1104 36-37 0.6413234858237189 1104 38-39 0.6094897128014125 1104 40-41 0.5253891853129531 1104 42-43 0.5036343585649874 1104 44-45 0.42014653189550444 1104 46-47 0.7181436858624153 1104 48-49 0.7128228510476831 1104 50-51 0.9021224030460715 1104 52-53 0.831298203554141 1104 54-55 0.7688989830361166 1104 56-57 0.6733208597764317 1104 58-59 0.71613653497554 1104 60-61 0.6715836816239644 1104 62-63 0.7976608856385567 1104 64-65 0.9112399113942331 1104 66-67 0.8167282590912279 1104 68-69 0.8440258095419111 1104 70-71 0.9023924997487072 1104 72-73 0.781939786922468 1104 74-75 0.7532088604776845 1104 76-77 0.6958527389265257 1104 78-79 0.5477314526254737 1104 80-81 0.6320971019787507 1104 82-83 0.5420813147978514 1104 84-85 0.6080950687432001 1104 86-87 0.6778778822590432 1104 88-89 0.7447227177870097 1104 90-91 0.7748557776308509 1104 92-93 0.8525742447971574 1104 94-95 0.8201253094104253 1104 96-97 0.7058241782679673 1104 98-99 0.7189917706337816 1104 100-101 1.0852017626835178 1105 1 0.028995140784804363 1105 2 0.10772863254580045 1105 3 0.11716936626849161 1105 4 0.16847453589477368 1105 5 0.09421146087147036 1105 6 0.18493811475767785 1105 7 0.19683271508002065 1105 8 0.19665551240900925 1105 9 0.22835577355088077 1105 10-11 0.25705982388225124 1105 12-13 0.2430631563496135 1105 14-15 0.327324204722899 1105 16-17 0.3570926665915266 1105 18-19 0.2843653707367082 1105 20-21 0.3590654571167633 1105 22-23 0.2309967983418204 1105 24-25 0.2385644323830789 1105 26-27 0.29855490341521573 1105 28-29 0.3553383975698452 1105 30-31 0.31650381414237927 1105 32-33 0.35253367820438797 1105 34-35 0.3135558446638953 1105 36-37 0.5295890622519934 1105 38-39 0.471355330508608 1105 40-41 0.4721950290656167 1105 42-43 0.3473007212334025 1105 44-45 0.4784742972549836 1105 46-47 0.6277882841743363 1105 48-49 0.5798289272454227 1105 50-51 0.6094391031205859 1105 52-53 0.6489497708802716 1105 54-55 0.5669335310724577 1105 56-57 0.5304586369636368 1105 58-59 0.49142914898258994 1105 60-61 0.5724296255191135 1105 62-63 0.5163170170479887 1105 64-65 0.479851904267683 1105 66-67 0.43088028492616814 1105 68-69 0.44913009189885855 1105 70-71 0.3124014849916037 1105 72-73 0.42690990593840894 1105 74-75 0.2963852300308716 1105 76-77 0.30462714654376555 1105 78-79 0.369070097423446 1105 80-81 0.5050766923151429 1105 82-83 0.35782824122491164 1105 84-85 0.3806941646937396 1105 86-87 0.3528846945366446 1105 88-89 0.5473741464132331 1105 90-91 0.7016511623826922 1105 92-93 0.6738026146028595 1105 94-95 0.6666319380676136 1105 96-97 0.7236861014204514 1105 98-99 0.7509476597236251 1105 100-101 1.1533590139092418 1106 1 0.012530981675887176 1106 2 0.0654661476103584 1106 3 0.002302220296328983 1106 4 0.03835047687521609 1106 5 0.07202797217681933 1106 6 0.10307180282702433 1106 7 0.0636556522513061 1106 8 0.07552385368731507 1106 9 0.026269508639401806 1106 10-11 0.05627013532566849 1106 12-13 0.11332796617302421 1106 14-15 0.18952146485162302 1106 16-17 0.1811984709026504 1106 18-19 0.13608727101933482 1106 20-21 0.19518292705500784 1106 22-23 0.16249311526094345 1106 24-25 0.0021807023049333907 1106 26-27 -0.01677495266388007 1106 28-29 -0.10805420156596313 1106 30-31 -0.14546536071737393 1106 32-33 -0.10706772356373762 1106 34-35 -0.17803500353244317 1106 36-37 0.004991300515328589 1106 38-39 -0.11382124114042114 1106 40-41 0.11309349354852571 1106 42-43 0.12518732751695438 1106 44-45 0.21577849631551516 1106 46-47 -0.03176914802939024 1106 48-49 -0.13223702666299886 1106 50-51 -0.13732988972664373 1106 52-53 -0.15459642816230001 1106 54-55 -0.28276028126841624 1106 56-57 -0.1053919265456571 1106 58-59 0.044614412014567506 1106 60-61 0.13900906507038258 1106 62-63 0.1776019032386742 1106 64-65 0.06955907178865317 1106 66-67 0.1468089113910267 1106 68-69 0.188577725067816 1106 70-71 0.19920559872007715 1106 72-73 0.1835942874978329 1106 74-75 0.03365213054464178 1106 76-77 0.10089810246663333 1106 78-79 0.16993918094831173 1106 80-81 0.12485437544991385 1106 82-83 0.1106496216063313 1106 84-85 0.14971401365489 1106 86-87 0.2500485480932433 1106 88-89 0.4062563818109872 1106 90-91 0.4401152860004842 1106 92-93 0.42793897414639304 1106 94-95 0.43938881660973905 1106 96-97 0.3473118334523555 1106 98-99 0.38445838297366564 1106 100-101 0.8732720672175738 1107 1 0.11572720945405379 1107 2 0.17019298895927548 1107 3 0.21293959481330305 1107 4 0.30551855591620836 1107 5 0.23695483275584905 1107 6 0.15724356937337802 1107 7 0.18581563645142296 1107 8 0.2118400644182401 1107 9 0.11484694755129254 1107 10-11 0.17682284534325987 1107 12-13 0.23582155396761806 1107 14-15 0.24121637900472592 1107 16-17 0.2938398707514054 1107 18-19 0.3326343601249562 1107 20-21 0.26940501674533124 1107 22-23 0.3145302735690052 1107 24-25 0.2568709674467087 1107 26-27 0.21517602092588817 1107 28-29 0.21137562538894628 1107 30-31 0.15558638389624235 1107 32-33 0.21629648190749862 1107 34-35 0.32689433877148133 1107 36-37 0.25716768229037257 1107 38-39 0.11485086713035031 1107 40-41 0.22110212371434557 1107 42-43 0.20890526324158287 1107 44-45 0.33561365001354915 1107 46-47 0.3841422816780309 1107 48-49 0.37352681187676495 1107 50-51 0.4233229468459285 1107 52-53 0.21437954198423625 1107 54-55 0.22488044292749265 1107 56-57 0.3058289131868932 1107 58-59 0.2924481000656556 1107 60-61 0.31549023904319284 1107 62-63 0.276254690436609 1107 64-65 0.3055100401810975 1107 66-67 0.09409986829736994 1107 68-69 0.2731282080296751 1107 70-71 0.24864649598706023 1107 72-73 0.2003335868458258 1107 74-75 0.13213113203367755 1107 76-77 0.10305791775353512 1107 78-79 0.09847200078548113 1107 80-81 -0.07178366692391336 1107 82-83 -0.12451460101608447 1107 84-85 0.004658168348210978 1107 86-87 -0.02211903720143482 1107 88-89 0.02880584089890803 1107 90-91 0.25146618890656924 1107 92-93 0.20385578308995989 1107 94-95 0.14028087327102767 1107 96-97 0.23982521007944513 1107 98-99 0.4586333718643729 1107 100-101 0.9380294948512571 1108 1 0.15952544020880666 1108 2 0.11463680601178794 1108 3 0.1541954766126601 1108 4 0.15177867261110833 1108 5 0.17760794775679045 1108 6 0.1522769779937434 1108 7 0.1472512660392269 1108 8 0.16487836669567457 1108 9 0.2751330382721662 1108 10-11 0.2572536283283924 1108 12-13 0.268655875443244 1108 14-15 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60-61 -1.141219664332226 2316 62-63 -1.5446661028698188 2316 64-65 -1.6615997644827623 2316 66-67 -1.540023670863011 2316 68-69 -1.7358387609318164 2316 70-71 -1.606497089385556 2316 72-73 -1.5025816709223854 2316 74-75 -1.5720366873256637 2316 76-77 -1.5555605740129685 2316 78-79 -1.4846838085429148 2316 80-81 -1.3823885608369864 2316 82-83 -1.3077017337177956 2316 84-85 -1.2940514972085104 2316 86-87 -1.347264939159647 2316 88-89 -1.2885011908810604 2316 90-91 -1.6289209459605267 2316 92-93 -1.3073416228568568 2316 94-95 -1.2676025085780687 2316 96-97 -1.5377872395240857 2316 98-99 -2.085036973338614 2316 100-101 -2.21714207248597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13188.0 3 2392.0 4 1962.0 5 2198.0 6 2588.0 7 3225.0 8 4217.0 9 5135.0 10 7051.0 11 10147.0 12 15474.0 13 20910.0 14 23222.0 15 29519.0 16 39232.0 17 51817.0 18 42301.0 19 23572.0 20 21194.0 21 21074.0 22 22642.0 23 25226.0 24 28390.0 25 32569.0 26 37079.0 27 43090.0 28 50345.0 29 58278.0 30 69729.0 31 82934.0 32 101670.0 33 127263.0 34 164455.0 35 227179.0 36 322201.0 37 525361.0 38 603876.0 39 120218.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.035813652833255 22.72779680923621 15.661506491480887 19.574883046449646 2 13.59456273904707 23.848393368637026 42.10384926644661 20.4531946258693 3 17.769272551195115 30.960771489889293 32.39701940851054 18.872936550405058 4 10.26648323327214 19.45049900755907 43.335736503323865 26.947281255844924 5 9.609676920698718 41.5365925786527 36.58592484558463 12.26780565506395 6 23.837332724243566 42.47084430590638 19.804307518675767 13.88751545117429 7 23.226354331579234 36.739677110508374 24.92767231028201 15.106296247630386 8 23.54235667558952 34.92388686962381 22.445971356122744 19.08778509866392 9 26.050606375385343 18.03363283350982 24.638864025666454 31.276896765438384 10-11 21.860278164429868 28.89099893525336 30.00501047361993 19.24371242669684 12-13 24.57137424301369 28.124044633384464 29.64001617950108 17.664564944100764 14-15 21.766438310161863 28.548492175431527 30.24993283632212 19.43513667808449 16-17 17.405477808768904 31.914318124968062 31.06247865063974 19.617725415623294 18-19 17.26606883819955 30.674030483270627 34.6149839734166 17.44491670511322 20-21 18.983905306203617 28.875487654374897 34.10517143734342 18.035435602078064 22-23 18.432622655988894 28.78946467228247 34.17881874459456 18.599093927134078 24-25 18.696974284213216 29.661520843515575 34.187854831368625 17.453650040902584 26-27 18.175383650157816 28.797642465890522 35.5683707599162 17.45860312403546 28-29 17.18021205691576 29.611978959100334 35.40286744643507 17.804941537548842 30-31 17.95995300930736 29.371317924851205 34.61382634558601 18.054902720255424 32-33 17.77407558244499 29.804187641963747 34.651607837868475 17.770128937722788 34-35 18.59828825173291 29.246011158192374 34.53180273241804 17.623897857656672 36-37 18.65970336988492 28.681626916669483 34.70039530374639 17.958274409699204 38-39 17.715155764490035 30.198155159930618 34.43304929304575 17.6536397825336 40-41 17.624557289516048 29.948429705471906 34.85091271902995 17.5761002859821 42-43 17.419419426156278 30.15321119286338 33.503751200905874 18.92361818007447 44-45 17.95367237152295 29.59613925211471 33.39456055891999 19.055627817442357 46-47 18.846744758580883 30.200384682048337 32.78625241267667 18.16661814669411 48-49 18.46998122485321 30.345131127274627 32.47908519338569 18.70580245448647 50-51 17.67867496148095 30.546032264280658 32.92531890239849 18.849973871839904 52-53 18.1426753097384 31.442446812610648 31.85147636206722 18.563401515583735 54-55 17.88380682736114 30.424683888547825 32.25201810505455 19.43949117903648 56-57 18.512050238124324 30.551234332974285 31.625157692376288 19.311557736525103 58-59 18.81088109483784 29.96144652892604 32.086126301305804 19.141546074930318 60-61 18.383261574885637 30.83869524897701 32.32003847295333 18.458004703184024 62-63 17.857083363130418 30.572448983884332 32.68990699102722 18.880560661958025 64-65 17.791753886427248 30.779884368311233 32.27784798748748 19.150513757774032 66-67 17.9501405745055 30.3286255210313 31.258713313185048 20.462520591278153 68-69 17.552361157100897 30.37442875638706 31.8927116663067 20.180498420205346 70-71 18.590323068288523 30.126638917478655 31.689643411823603 19.593394602409216 72-73 18.32656185220727 29.366704781321584 32.204970099381455 20.10176326708969 74-75 17.477848624327276 31.1377261635174 31.293335348650412 20.09108986350491 76-77 17.777153605640304 29.807594731351394 31.83689652787326 20.578355135135045 78-79 17.742000068644387 29.725627709181996 31.897499123438088 20.63487309873553 80-81 18.262966594385343 30.01060241969395 31.09633663767842 20.630094348242288 82-83 18.179261809681996 29.47769997492583 31.766130348665598 20.576907866726575 84-85 17.632980437566967 29.581635845293093 31.447813906901285 21.33756981023866 86-87 17.57498321048507 29.76857140789779 31.596663938948932 21.059781442668204 88-89 18.076409211029635 29.07135934428977 31.244718861244703 21.60751258343589 90-91 18.001021398534963 28.462714408688015 31.708398461236513 21.82786573154051 92-93 18.63908940917371 29.101383721818824 31.345441642677734 20.914085226329732 94-95 18.155444175514805 28.12534193698697 31.610633049301885 22.108580838196342 96-97 17.904246080170385 27.924475449605605 31.84087935218883 22.330399118035178 98-99 18.1655402880396 28.350788442594414 30.893961052908708 22.58971021645727 100-101 18.150074249194567 27.271416146877424 31.663208884592652 22.915300719335352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6544.0 1 4086.5 2 1763.0 3 1891.0 4 1693.0 5 1819.0 6 2858.5 7 4894.0 8 7294.0 9 8185.5 10 7391.5 11 6862.0 12 7219.0 13 7757.0 14 8319.0 15 8733.5 16 9204.0 17 9955.5 18 10665.5 19 11520.5 20 12677.0 21 14210.0 22 16141.0 23 18177.5 24 20352.0 25 24015.0 26 31329.5 27 39317.0 28 42226.5 29 47466.0 30 59004.5 31 69571.5 32 84828.0 33 105646.5 34 125182.5 35 138486.5 36 155223.5 37 168862.0 38 161882.5 39 151296.0 40 139340.0 41 125544.0 42 112128.0 43 105106.0 44 102787.0 45 96802.0 46 89714.5 47 93433.0 48 125088.0 49 117448.5 50 71988.0 51 46344.0 52 31450.5 53 24389.0 54 19562.5 55 15718.0 56 12445.5 57 9681.0 58 7516.0 59 5748.0 60 4334.0 61 3340.0 62 2629.5 63 2090.0 64 1580.0 65 1143.5 66 824.5 67 729.5 68 576.5 69 392.5 70 314.0 71 269.0 72 241.0 73 198.5 74 164.5 75 149.0 76 117.0 77 84.0 78 62.0 79 48.5 80 33.0 81 28.0 82 26.0 83 18.5 84 14.5 85 10.5 86 8.5 87 7.5 88 4.0 89 2.0 90 2.0 91 0.5 92 0.0 93 0.5 94 1.0 95 2.0 96 1.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27026836757168465 2 0.16731697129127734 3 0.20006966232545448 4 0.1659424980748994 5 0.24207490476744414 6 0.1958121477283813 7 0.24365052040573107 8 0.24995298295887877 9 0.14894596342359154 10-11 0.2077298362264877 12-13 0.21304334853858298 14-15 0.17301600657869814 16-17 0.28941042139002154 18-19 0.2335933993102826 20-21 0.19963385374465173 22-23 0.24715375092064562 24-25 0.23424711218148678 26-27 0.34742324824226667 28-29 0.31894483367365517 30-31 0.2935003173021706 32-33 0.1927112020572847 34-35 0.36188874075122 36-37 0.40075951378512825 38-39 0.3813492700709128 40-41 0.34269640132740586 42-43 0.37700794613137756 44-45 0.3860593551172811 46-47 0.39142315303485364 48-49 0.4116212045682126 50-51 0.4032402703220055 52-53 0.4031732228480359 54-55 0.39749094942910757 56-57 0.37526471180816645 58-59 0.35085943128321156 60-61 0.31658141021622477 62-63 0.3846345962954259 64-65 0.41887909362542786 66-67 0.4240752728580762 68-69 0.42898650032635355 70-71 0.4285674536140432 72-73 0.4203038524472831 74-75 0.414956816398203 76-77 0.39941856430573514 78-79 0.3728845264822437 80-81 0.3845005013474866 82-83 0.39477552673333643 84-85 0.39495990728675295 86-87 0.389294395736317 88-89 0.39868104209206895 90-91 0.4198512819979879 92-93 0.4188455698884431 94-95 0.42742764655655907 96-97 0.42824897811268736 98-99 0.424913366282697 100-101 0.3984296140646827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2982961.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.527919610098955 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9408692080786 18.620286454345724 2 12.017702165619584 6.135738695640976 3 4.94830017817143 3.789594274649957 4 2.6029189494702383 2.6578842277471786 5 1.569407755995608 2.0031857515260834 6 1.0304874556023453 1.5783720555499252 7 0.7254451428510691 1.296337370176719 8 0.5432030972942914 1.1093475999748346 9 0.42092016948016137 0.9670694623882907 >10 2.4276056037688063 11.802509549458302 >50 0.3192891078137658 5.759852084943783 >100 0.38042088484646375 20.66459717299478 >500 0.048379156259601326 8.390754361108716 >1k 0.023328031511478994 10.066523168878112 >5k 9.278194351156418E-4 1.493324325850957 >10k+ 7.95273801527693E-4 3.6646234447656663 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26488 0.8879767452541284 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24341 0.8160012819477023 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19331 0.6480473596537132 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14084 0.47214831169431987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13101 0.43919447823823377 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10960 0.3674201573537167 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8912 0.29876354400878863 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6909 0.23161549882817775 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6205 0.20801478799085873 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5819 0.19507462551471508 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5645 0.189241495279355 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5556 0.18625788268770527 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5088 0.1705687737788057 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4907 0.16450097738455177 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4861 0.16295888548324972 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4677 0.1567905178780413 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4487 0.15042100785092397 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4195 0.14063207665135413 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3724 0.12484239653150007 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3498 0.11726603197292891 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3466 0.11619327238941442 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3332 0.11170109163344744 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3327 0.1115334729485233 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3106 0.10412472707487627 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3071 0.1029513962804073 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3066 0.10278377759548314 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3056 0.10244854022563486 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 3019 0.10120816195719623 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2985 0.10006835489971205 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.352373698482816E-5 0.0 0.0 6.704747396965633E-5 0.0 12-13 3.352373698482816E-5 0.0 0.0 1.0057121095448448E-4 0.0 14-15 3.352373698482816E-5 0.0 0.0 1.0057121095448448E-4 0.0 16-17 3.352373698482816E-5 0.0 0.0 2.3466615889379712E-4 0.0 18-19 3.352373698482816E-5 0.0 0.0 3.519992383406957E-4 0.0 20-21 3.352373698482816E-5 0.0 0.0 4.8609418628000836E-4 0.0 22-23 3.352373698482816E-5 0.0 0.0 0.0010727595835145012 3.352373698482816E-5 24-25 3.352373698482816E-5 0.0 0.0 0.0025813277478317683 3.352373698482816E-5 26-27 3.352373698482816E-5 0.0 0.0 0.004676561309383528 3.352373698482816E-5 28-29 3.352373698482816E-5 0.0 0.0 0.012353497078909178 3.352373698482816E-5 30-31 6.704747396965633E-5 0.0 0.0 0.038334393242151 3.352373698482816E-5 32-33 6.704747396965633E-5 0.0 0.0 0.09128513580968708 3.352373698482816E-5 34-35 6.704747396965633E-5 0.0 0.0 0.16681411523650494 3.352373698482816E-5 36-37 6.704747396965633E-5 0.0 0.0 0.2759674028591054 3.352373698482816E-5 38-39 6.704747396965633E-5 0.0 0.0 0.44863141019946284 3.352373698482816E-5 40-41 6.704747396965633E-5 0.0 0.0 0.657752481510821 3.352373698482816E-5 42-43 8.38093424620704E-5 0.0 0.0 0.8586267135239113 3.352373698482816E-5 44-45 1.0057121095448448E-4 0.0 0.0 1.0951534398203666 3.352373698482816E-5 46-47 1.1733307944689856E-4 0.0 0.0 1.3885196621745977 3.352373698482816E-5 48-49 1.3409494793931265E-4 0.0 0.0 1.699586417656818 3.352373698482816E-5 50-51 1.3409494793931265E-4 0.0 0.0 2.0495742317784242 3.352373698482816E-5 52-53 1.3409494793931265E-4 0.0 0.0 2.3816771322186243 3.352373698482816E-5 54-55 1.3409494793931265E-4 0.0 0.0 2.7320672311840486 3.352373698482816E-5 56-57 1.676186849241408E-4 0.0 0.0 3.1517508944971118 3.352373698482816E-5 58-59 1.676186849241408E-4 0.0 0.0 3.61208208890428 3.352373698482816E-5 60-61 1.8438055341655487E-4 0.0 0.0 4.135287052026493 3.352373698482816E-5 62-63 3.0171363286345343E-4 0.0 0.0 4.648015847340948 3.352373698482816E-5 64-65 4.358085808027661E-4 0.0 0.0 5.162823114348461 3.352373698482816E-5 66-67 4.693323177875943E-4 0.0 0.0 5.730765504476928 3.352373698482816E-5 68-69 4.693323177875943E-4 0.0 0.0 6.358799193150698 3.352373698482816E-5 70-71 4.693323177875943E-4 0.0 0.0 7.066468519031929 3.352373698482816E-5 72-73 4.693323177875943E-4 0.0 0.0 7.757443023894714 3.352373698482816E-5 74-75 4.860941862800084E-4 0.0 0.0 8.46390549524449 3.352373698482816E-5 76-77 5.196179232648365E-4 0.0 0.0 9.178296330391179 3.352373698482816E-5 78-79 5.363797917572506E-4 0.0 0.0 10.00075093170846 3.352373698482816E-5 80-81 5.363797917572506E-4 0.0 0.0 10.901818696255164 3.352373698482816E-5 82-83 5.363797917572506E-4 0.0 0.0 11.768005012469153 3.352373698482816E-5 84-85 5.363797917572506E-4 0.0 0.0 12.62798943734095 3.352373698482816E-5 86-87 5.363797917572506E-4 0.0 0.0 13.506763916792742 3.352373698482816E-5 88-89 5.363797917572506E-4 0.0 0.0 14.428113542215268 3.352373698482816E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCGC 40 5.423568E-4 47.43945 9 TCACGCA 65 0.0058796788 29.195961 4 ATCACGC 65 0.0058796788 29.195961 3 CATTCCG 685 0.0 29.086962 9 GTAAGCG 210 0.0 28.282207 94-95 CATGGGT 2790 0.0 27.377754 4 GTATGGT 695 0.0 27.3474 1 GTACATG 22190 0.0 26.48825 1 TGTACGC 45 8.9364493E-4 26.396727 92-93 AGGCTTA 1760 0.0 25.337406 6 CATGGGG 7365 0.0 25.05838 4 ACATGGG 21150 0.0 24.944256 3 TACATGG 22925 0.0 24.937134 2 GAGTACT 20080 0.0 24.563326 12-13 GACATAT 1285 0.0 24.035387 1 ACCAGCG 100 0.0016660987 23.720922 8 TACCTGG 1005 0.0 23.603302 2 CTTTGCG 465 0.0 23.466253 2 AGTACTT 21620 0.0 23.186947 12-13 GACGTGA 205 2.7079295E-8 23.142363 7 >>END_MODULE