##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938780_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2982961 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.994196370653185 31.0 31.0 34.0 30.0 34.0 2 32.18897665775717 33.0 31.0 34.0 30.0 34.0 3 32.248910059501284 34.0 31.0 34.0 30.0 34.0 4 35.902098284221616 37.0 35.0 37.0 35.0 37.0 5 35.82416095952981 37.0 35.0 37.0 35.0 37.0 6 35.86676895876278 37.0 35.0 37.0 35.0 37.0 7 35.79534864854083 37.0 35.0 37.0 33.0 37.0 8 35.79202711668037 37.0 35.0 37.0 33.0 37.0 9 37.39264811038428 39.0 37.0 39.0 34.0 39.0 10-11 37.32445647127133 39.0 37.0 39.0 34.0 39.0 12-13 37.27038016923453 39.0 37.0 39.0 33.0 39.0 14-15 38.383759626760124 40.0 38.0 41.0 33.0 41.0 16-17 38.35276374716263 40.0 38.0 41.0 33.0 41.0 18-19 38.588966801778504 40.0 38.0 41.0 34.0 41.0 20-21 38.6260976928629 40.0 38.0 41.0 34.0 41.0 22-23 38.53879450653227 40.0 38.0 41.0 34.0 41.0 24-25 38.45413382876947 40.0 38.0 41.0 34.0 41.0 26-27 38.25535633888609 40.0 38.0 41.0 33.5 41.0 28-29 38.10013825189132 40.0 37.0 41.0 33.0 41.0 30-31 37.832725436236004 40.0 37.0 41.0 33.0 41.0 32-33 37.58180965155093 40.0 36.0 41.0 33.0 41.0 34-35 37.4671507606033 40.0 36.0 41.0 33.0 41.0 36-37 37.29353970769313 40.0 36.0 41.0 32.0 41.0 38-39 37.131084851595446 40.0 36.0 41.0 31.5 41.0 40-41 36.95535191375281 40.0 36.0 41.0 31.0 41.0 42-43 36.66727137900898 40.0 35.0 41.0 30.5 41.0 44-45 36.26590441510969 40.0 35.0 41.0 30.0 41.0 46-47 35.743924074099525 39.0 35.0 41.0 28.0 41.0 48-49 35.27610652636759 39.0 35.0 41.0 25.0 41.0 50-51 35.018662664379455 39.0 35.0 41.0 24.0 41.0 52-53 34.85407670432164 39.0 35.0 41.0 23.5 41.0 54-55 34.63478201692882 39.0 35.0 40.0 23.0 41.0 56-57 34.402265902906535 39.0 34.0 40.0 22.0 41.0 58-59 34.15788372694112 38.0 34.0 40.0 20.5 41.0 60-61 33.814013659581875 38.0 34.0 40.0 18.5 41.0 62-63 33.514953095263394 38.0 33.5 40.0 16.5 41.0 64-65 33.15532921818287 37.5 33.0 40.0 13.0 41.0 66-67 32.759551499332375 37.0 32.5 39.5 8.0 41.0 68-69 32.42491688627508 36.5 32.0 39.0 7.5 40.5 70-71 32.00167836589215 36.0 31.5 39.0 4.5 40.0 72-73 31.503896296331064 35.5 31.0 38.5 2.0 40.0 74-75 30.99803249187636 35.0 31.0 37.5 2.0 39.5 76-77 29.01450454766254 33.0 28.5 35.5 2.0 38.5 78-79 29.929519695363098 34.0 29.5 36.0 2.0 39.0 80-81 29.922082454313013 34.0 30.0 36.0 2.0 38.5 82-83 29.558813038454073 34.0 30.0 36.0 2.0 37.0 84-85 29.111548223392795 34.0 29.0 35.0 2.0 37.0 86-87 28.563751923005363 34.0 29.0 35.0 2.0 36.5 88-89 27.94292516730859 34.0 28.0 35.0 2.0 36.0 90-91 27.41076333213877 33.5 26.0 35.0 2.0 35.5 92-93 27.122349571449305 33.0 26.0 35.0 2.0 35.0 94-95 26.78253570864654 33.0 26.0 35.0 2.0 35.0 96-97 26.19153317793964 33.0 25.0 35.0 2.0 35.0 98-99 25.44776733587868 33.0 22.0 35.0 2.0 35.0 100-101 22.0128706007219 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09200529831952764 1101 2 0.15859580656029948 1101 3 0.09412748425058481 1101 4 0.1323473030767488 1101 5 -0.07082724201585933 1101 6 0.10020593189128135 1101 7 0.2314529064794968 1101 8 0.18024353402041982 1101 9 0.16188405046823817 1101 10-11 0.3262820302563938 1101 12-13 0.27706990957316435 1101 14-15 0.3307684508739186 1101 16-17 0.3892789679998643 1101 18-19 0.41769222171722475 1101 20-21 0.3464482917619591 1101 22-23 0.21145586616157885 1101 24-25 0.23712635604587717 1101 26-27 0.22262560960563604 1101 28-29 0.20807455715361556 1101 30-31 0.20916575383996872 1101 32-33 0.3478230298075715 1101 34-35 0.3631658598701861 1101 36-37 0.41961190809322346 1101 38-39 0.37889135649504624 1101 40-41 0.2907799395548878 1101 42-43 0.25230386803571747 1101 44-45 0.21727726993941587 1101 46-47 0.2591567548987186 1101 48-49 0.2877038977899744 1101 50-51 0.23961543042887712 1101 52-53 0.11250317644184094 1101 54-55 0.13579902218354079 1101 56-57 0.13794044399459437 1101 58-59 0.22183052967372419 1101 60-61 0.12580785072677259 1101 62-63 0.0786295119940732 1101 64-65 0.07181828080337027 1101 66-67 0.010256124932645605 1101 68-69 0.009685084127525556 1101 70-71 0.13328418987141077 1101 72-73 0.0776721916487162 1101 74-75 -0.012896401470460006 1101 76-77 -0.059999453556795146 1101 78-79 -0.10436993431932962 1101 80-81 0.12015079191875344 1101 82-83 0.13072150543440486 1101 84-85 0.03676145791934715 1101 86-87 0.05113141363046125 1101 88-89 0.09548904064301311 1101 90-91 0.08633408982473512 1101 92-93 -0.005206243009983069 1101 94-95 0.04613985641824314 1101 96-97 0.07131998971167874 1101 98-99 0.06366970724713994 1101 100-101 0.9166422757805641 1102 1 0.3177273999864383 1102 2 0.2489906700661635 1102 3 0.19515998091046072 1102 4 0.10761951092830913 1102 5 0.057355092373555294 1102 6 -0.0335562309248445 1102 7 0.1404161554633916 1102 8 0.19091813451795048 1102 9 0.24150312004127272 1102 10-11 0.2550139084787446 1102 12-13 0.2697799992183505 1102 14-15 0.32318062356045374 1102 16-17 0.3481312651655273 1102 18-19 0.31977388145884333 1102 20-21 0.35307565272429287 1102 22-23 0.26576543691969334 1102 24-25 0.2830844188973316 1102 26-27 0.20813708703227718 1102 28-29 0.20976192345816713 1102 30-31 0.2733434271078963 1102 32-33 0.30608372500771 1102 34-35 0.42632718012961135 1102 36-37 0.3832642965694717 1102 38-39 0.46198759680419244 1102 40-41 0.4491792809636195 1102 42-43 0.5374296279511199 1102 44-45 0.5093556287586409 1102 46-47 0.549523226986885 1102 48-49 0.4868795882640242 1102 50-51 0.5365318436555597 1102 52-53 0.44759534212823127 1102 54-55 0.46623174672156864 1102 56-57 0.5405231543804803 1102 58-59 0.5996482316293097 1102 60-61 0.6071012546822558 1102 62-63 0.5638722604824622 1102 64-65 0.5165644246862371 1102 66-67 0.6782165656431332 1102 68-69 0.584279084365015 1102 70-71 0.4988125527856724 1102 72-73 0.4997857951581466 1102 74-75 0.4576718675044269 1102 76-77 0.34504886661973444 1102 78-79 0.4409665917311969 1102 80-81 0.5717363922298304 1102 82-83 0.5423292264063875 1102 84-85 0.6740359540121368 1102 86-87 0.796106344294909 1102 88-89 0.9179355120540613 1102 90-91 0.8129709154227669 1102 92-93 0.8880966061640017 1102 94-95 0.9300810059009841 1102 96-97 1.0560175632349917 1102 98-99 1.2614366556539203 1102 100-101 1.646799400682145 1103 1 0.19431716506874608 1103 2 0.16602698260414428 1103 3 0.14755581155800002 1103 4 0.044275919293674804 1103 5 -0.051099200861756344 1103 6 -0.036309966991368015 1103 7 0.037246403603688805 1103 8 0.07444137453252608 1103 9 0.08871759232172849 1103 10-11 0.09782473817034543 1103 12-13 0.20544471233213102 1103 14-15 0.17308267024237267 1103 16-17 0.10282813528093726 1103 18-19 -0.03023786710153331 1103 20-21 0.1600021101417184 1103 22-23 0.21480994815282628 1103 24-25 0.16502263703719677 1103 26-27 0.0979329758631664 1103 28-29 -0.011716925971022363 1103 30-31 0.1651092673537491 1103 32-33 0.28367687309162903 1103 34-35 0.09102503050791455 1103 36-37 0.18460702567005427 1103 38-39 0.12119883300084666 1103 40-41 0.2606804404954417 1103 42-43 0.2780622186035444 1103 44-45 0.2361506409474714 1103 46-47 0.28400752599674206 1103 48-49 0.1912059044068357 1103 50-51 0.2626797120580662 1103 52-53 0.31333380360681673 1103 54-55 0.15578078200179135 1103 56-57 0.11211749483381084 1103 58-59 0.17443681835289482 1103 60-61 0.18920417158465597 1103 62-63 0.1987245715921233 1103 64-65 0.19645160688524754 1103 66-67 0.14798512386855833 1103 68-69 0.004600128979866724 1103 70-71 0.09765406689538025 1103 72-73 0.10641413589656423 1103 74-75 0.21497470452635525 1103 76-77 0.2826099506202375 1103 78-79 0.11199929991787272 1103 80-81 0.185837717360414 1103 82-83 0.17336617429094403 1103 84-85 0.16652538882124546 1103 86-87 0.15752775712861933 1103 88-89 0.10193487281562241 1103 90-91 0.15179288994955087 1103 92-93 0.22472192827644832 1103 94-95 0.3132107421154018 1103 96-97 0.4479654696804545 1103 98-99 0.6034294644647069 1103 100-101 1.0256422501872215 1104 1 0.185321864776391 1104 2 0.19333467874958643 1104 3 0.13777473212196867 1104 4 0.09377667929593514 1104 5 -0.0058067247178499315 1104 6 0.02928138794132451 1104 7 0.07601233953732844 1104 8 0.09470948736542084 1104 9 0.1464095802016061 1104 10-11 0.19168085993035788 1104 12-13 0.05336692934353238 1104 14-15 0.1319361145327136 1104 16-17 0.17243403170110838 1104 18-19 0.1739216901347831 1104 20-21 0.2779924612727456 1104 22-23 0.2198335298422549 1104 24-25 0.27726224895377527 1104 26-27 0.225568869921851 1104 28-29 0.25663704549813104 1104 30-31 0.1414228073677819 1104 32-33 0.09707428276443864 1104 34-35 0.13243475986728726 1104 36-37 0.22404025024156482 1104 38-39 0.40120229209210834 1104 40-41 0.3597143894805015 1104 42-43 0.42796850716492685 1104 44-45 0.4089862975262264 1104 46-47 0.37184391430634633 1104 48-49 0.4351375274856295 1104 50-51 0.26357940838976646 1104 52-53 0.30230587144492205 1104 54-55 0.22986852983402883 1104 56-57 0.1965750328256135 1104 58-59 0.3136893552969937 1104 60-61 0.42061529788472996 1104 62-63 0.38130305804013886 1104 64-65 0.20846321812331325 1104 66-67 0.14966020543991476 1104 68-69 0.23443429216249712 1104 70-71 0.2751162580301241 1104 72-73 0.32043418199560847 1104 74-75 0.3614597356415459 1104 76-77 0.35380528284267143 1104 78-79 0.30229380495185154 1104 80-81 0.3328120934767185 1104 82-83 0.27341623133268556 1104 84-85 0.48065910265461653 1104 86-87 0.6050755441417266 1104 88-89 0.6668219368121377 1104 90-91 0.6965918635307418 1104 92-93 0.717508558888742 1104 94-95 0.6516890579370838 1104 96-97 0.7657270717718738 1104 98-99 1.0735598788571892 1104 100-101 1.6460065689956984 1105 1 0.09093160842812864 1105 2 0.11956824323802095 1105 3 0.04104404317381238 1105 4 0.05460245862106916 1105 5 -0.11304595832262976 1105 6 -0.07387881251258221 1105 7 0.058319938500289936 1105 8 0.0760791781125576 1105 9 0.0974398351617225 1105 10-11 0.09035127001062904 1105 12-13 0.09341005074683295 1105 14-15 0.08365062239516874 1105 16-17 0.09175577428799642 1105 18-19 0.05932451178188103 1105 20-21 -0.04428295017797268 1105 22-23 -0.04756289243059797 1105 24-25 -0.002184398897654205 1105 26-27 0.06136986305887859 1105 28-29 0.04561209195689031 1105 30-31 0.026550968940973974 1105 32-33 0.045108048830087455 1105 34-35 0.007070872370157133 1105 36-37 -0.012100232335953365 1105 38-39 -0.0012591918554036852 1105 40-41 0.004993015915289334 1105 42-43 0.13092026961844283 1105 44-45 0.19566748715823223 1105 46-47 0.12804662576977677 1105 48-49 0.15542506667406286 1105 50-51 0.06846128001437535 1105 52-53 -0.008737815146247385 1105 54-55 0.19957732508658665 1105 56-57 0.23444045144420755 1105 58-59 0.16809554329941534 1105 60-61 0.1581975746158335 1105 62-63 0.259116368870977 1105 64-65 0.23090908777119523 1105 66-67 0.10521441775626528 1105 68-69 0.08372759042244837 1105 70-71 0.05211134211708668 1105 72-73 0.07077142826466343 1105 74-75 0.04282739440642658 1105 76-77 0.06500004389011949 1105 78-79 -0.15489792250590284 1105 80-81 -0.07279388301689949 1105 82-83 0.08902662681693485 1105 84-85 0.09907538024805618 1105 86-87 0.12488971137318217 1105 88-89 0.012349732217547427 1105 90-91 0.054500367022612295 1105 92-93 0.170968117133512 1105 94-95 0.3284878388390524 1105 96-97 0.4190598251911659 1105 98-99 0.5668307070463534 1105 100-101 1.2807589565463111 1106 1 -0.019937089514112216 1106 2 -0.014114239546039187 1106 3 0.008605371076569668 1106 4 -0.01826893357978321 1106 5 -0.07984926583288399 1106 6 -0.06798213682635179 1106 7 -0.08231847679016369 1106 8 -0.06205064974889751 1106 9 -0.0886125458000464 1106 10-11 -0.06608874855866986 1106 12-13 0.011783796863888085 1106 14-15 -0.10996301198179737 1106 16-17 0.027571054796069916 1106 18-19 -0.20308659334831702 1106 20-21 -0.21328721152325159 1106 22-23 -0.08644030147308257 1106 24-25 -0.11324698355878127 1106 26-27 -0.09055270450077302 1106 28-29 0.0650923944902857 1106 30-31 -0.13671512184928503 1106 32-33 -0.013683771823160384 1106 34-35 -0.0437899408455138 1106 36-37 -0.1272866341511687 1106 38-39 0.07339809419936216 1106 40-41 0.16673994401574532 1106 42-43 0.09022602152537473 1106 44-45 -0.0091611794203601 1106 46-47 0.13459753220661952 1106 48-49 0.1033243643221553 1106 50-51 0.194603650703435 1106 52-53 0.05069076454481092 1106 54-55 0.06776929966618184 1106 56-57 0.06884695324362866 1106 58-59 0.09133874888440374 1106 60-61 0.0036943967358311625 1106 62-63 0.11707775189452008 1106 64-65 0.14749677572355324 1106 66-67 0.09942262711711436 1106 68-69 0.09450785630357217 1106 70-71 -0.006559162411207353 1106 72-73 0.09176656663734306 1106 74-75 0.3052225613910906 1106 76-77 0.22927971310403805 1106 78-79 0.12632452120724835 1106 80-81 0.13554539288959688 1106 82-83 0.31351162754318906 1106 84-85 0.23807227608882187 1106 86-87 0.37247971041141525 1106 88-89 0.331234616305057 1106 90-91 0.09316488406613033 1106 92-93 0.26624235291860643 1106 94-95 0.35447002842696307 1106 96-97 0.4379747343349756 1106 98-99 0.5134706721160747 1106 100-101 1.04758049159895 1107 1 0.035055855110940115 1107 2 0.06401396818083072 1107 3 0.016069386065666436 1107 4 0.02862462251110287 1107 5 -0.018718924602204368 1107 6 0.03737812906903315 1107 7 0.10523041059149563 1107 8 0.09719423023177143 1107 9 0.10076580160505699 1107 10-11 0.01829184669148276 1107 12-13 0.07908401801027054 1107 14-15 0.067848028705491 1107 16-17 0.05604007741224848 1107 18-19 0.08347044864350295 1107 20-21 0.03874202085714984 1107 22-23 0.09187942784999592 1107 24-25 0.059238418441665885 1107 26-27 0.10249268377872056 1107 28-29 0.04983433408171578 1107 30-31 0.16479496226950374 1107 32-33 0.033302036868334994 1107 34-35 0.08540203031970606 1107 36-37 -0.05195848775279188 1107 38-39 -0.07302594812769314 1107 40-41 -0.002737828304844925 1107 42-43 -0.01525020978697711 1107 44-45 0.13618928950870668 1107 46-47 -0.036033397621643815 1107 48-49 -0.021357299239099348 1107 50-51 -0.12573375993497393 1107 52-53 -0.13239329186700388 1107 54-55 0.009106065835716493 1107 56-57 0.09272445998066559 1107 58-59 -0.03753683484067949 1107 60-61 0.07900597664606579 1107 62-63 0.03590585801359225 1107 64-65 -0.08649674840375354 1107 66-67 -0.05885457505640801 1107 68-69 -0.03250545925488524 1107 70-71 -0.09689849606157708 1107 72-73 0.0066976500466893185 1107 74-75 0.09655432513087803 1107 76-77 0.09198802857155286 1107 78-79 0.1180964987651798 1107 80-81 0.05947565252219533 1107 82-83 0.07552135694422546 1107 84-85 -0.026478947492503124 1107 86-87 0.011758723854310205 1107 88-89 0.022738671521487674 1107 90-91 0.19487370107595225 1107 92-93 0.22410565275514926 1107 94-95 0.2598065165393706 1107 96-97 0.5541249813380666 1107 98-99 0.8036856596554749 1107 100-101 1.1982208675737738 1108 1 0.11125197782880036 1108 2 0.04277455541967612 1108 3 0.0075280723069610644 1108 4 0.03585358919659143 1108 5 -0.0169209761738216 1108 6 -0.007419537032980372 1108 7 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-1.8104240079919585 2316 100-101 -1.793314774803651 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 18.0 5 66.0 6 249.0 7 604.0 8 1329.0 9 2534.0 10 4632.0 11 7540.0 12 11657.0 13 16087.0 14 19358.0 15 26729.0 16 36135.0 17 51524.0 18 44477.0 19 23773.0 20 18702.0 21 18670.0 22 19961.0 23 21396.0 24 24394.0 25 28793.0 26 34028.0 27 41179.0 28 49695.0 29 60183.0 30 71867.0 31 86682.0 32 106673.0 33 132359.0 34 173180.0 35 236820.0 36 342463.0 37 586838.0 38 612413.0 39 69944.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.43331914182958 23.68140824520768 16.26373212802999 19.621540484932755 2 13.820629904313197 24.54225851427491 41.127456912778946 20.509654668632944 3 17.693191429589593 30.836138990754485 32.59885060515374 18.87181897450218 4 10.522363517323893 20.175322439683256 42.42874781131902 26.873566231673827 5 9.55458972739927 41.97980166505316 36.446771046303525 12.018837561244037 6 23.8529563416366 42.9900745664705 19.297334555434045 13.859634536458854 7 23.121824254490754 36.88201086102031 24.5334417714479 15.462723113041035 8 23.657500047771325 35.444311876688964 21.98231891063946 18.915869164900244 9 25.442404376054533 18.209054694312126 24.38117695806281 31.967363971570528 10-11 21.866377066277433 29.21410973861207 29.676452357238325 19.243060837872168 12-13 24.41748316521738 28.25952132796909 29.465655099077733 17.8573404077358 14-15 21.43683742429083 28.92577207680556 30.002990317339044 19.63440018156456 16-17 17.369725424987408 32.10044311165458 30.870491881594397 19.659339581763614 18-19 17.184887767557136 30.89891218825858 34.41295410835073 17.503245935833554 20-21 18.844513890728038 29.055425129594386 33.96789297614015 18.132168003537423 22-23 18.32704148663023 29.02267243856021 33.942666364059065 18.707619710750492 24-25 18.630812239289213 29.937920535737934 34.010380995195696 17.420886229777157 26-27 18.155552016896166 28.965428229003408 35.41279113621697 17.466228617883452 28-29 17.067202688871895 29.91239241813755 35.192682706880845 17.827722186109707 30-31 17.90843058290068 29.49802226713658 34.46669265873741 18.12685449122533 32-33 17.696879040657922 29.990167487942347 34.5009539179359 17.811999553463824 34-35 18.47642787217208 29.39031299869919 34.451447652509756 17.681811476618968 36-37 18.542400193666218 28.913400421331314 34.58886667375256 17.95533271124991 38-39 17.559760253218414 30.43557999687182 34.34599129238992 17.65866845751985 40-41 17.568783132643222 30.115116866954462 34.633075044556826 17.683024955845493 42-43 17.383158895587457 30.265435041636408 33.38011939413811 18.97128666863802 44-45 17.961594119450506 29.77028181600117 33.18654421332883 19.081579851219495 46-47 18.840519950029176 30.440047329137137 32.488529355513464 18.230903365320227 48-49 18.415101626414874 30.539250171937777 32.314160951191795 18.731487250455555 50-51 17.639036789122752 30.705770324481335 32.65422053024169 19.00097235615423 52-53 18.085368866706606 31.67163432575887 31.508591630933157 18.73440517660137 54-55 17.840193016268064 30.665922216214025 31.876548168078635 19.61733659943928 56-57 18.43933696612167 30.74301746490734 31.29789238715227 19.519753181818718 58-59 18.702658070710466 30.214002701349973 31.727536437885544 19.355802790054017 60-61 18.242877077999086 31.252284908498893 31.848150491763434 18.656687521738583 62-63 17.780551328739875 30.894161764622076 32.25345875326314 19.07182815337491 64-65 17.623835432422688 31.177213740694732 31.746253378190936 19.452697448691644 66-67 17.885447978109173 30.688443282524286 30.689013216432066 20.737095522934474 68-69 17.458582205509497 30.706694732776906 31.381478177526528 20.453244884187065 70-71 18.48985134889596 30.488562024674838 31.186560454421418 19.835026172007787 72-73 18.300892971782066 29.763060931738632 31.56950426103459 20.366541835444714 74-75 17.339737293266456 31.528612988636066 30.625780687950773 20.505869030146705 76-77 17.599683212846987 30.10059937645527 31.415732319551832 20.88398509114591 78-79 17.53958826293042 30.010606554242898 31.412790003821982 21.037015179004694 80-81 18.02812566890948 30.353995487998642 30.619958507288214 20.997920335803663 82-83 17.875559515859543 29.95593938321171 31.163021935435424 21.005479165493327 84-85 17.311138161042 29.949235005083874 30.78928956831819 21.950337265555937 86-87 17.17677195306897 30.22461076785276 30.92400874293613 21.674608536142134 88-89 17.685310207114252 29.51711581203533 30.575151773382252 22.22242220746817 90-91 17.67742096156121 28.933681296593356 30.982758385689646 22.406139356155787 92-93 18.17985551216078 29.69721249099046 30.64181431133609 21.481117685512665 94-95 17.72236132766601 28.663463572457992 30.820573868343036 22.793601231532957 96-97 17.279545551688216 28.576823504860528 31.059275903193388 23.084355040257865 98-99 17.435745891414605 28.940036426892608 30.146237245475216 23.47798043621757 100-101 17.310055757296187 27.98797020913679 31.13491173772884 23.567062295838184 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4449.0 1 2693.0 2 1117.0 3 1303.5 4 1097.0 5 1180.5 6 2221.0 7 4210.0 8 6240.5 9 7036.5 10 6447.5 11 5853.0 12 6265.0 13 6839.0 14 7282.5 15 7846.5 16 8546.0 17 9387.0 18 10199.0 19 11117.0 20 12439.0 21 14098.0 22 15882.0 23 17811.5 24 19764.0 25 23404.5 26 31223.0 27 39242.0 28 42165.5 29 47727.5 30 59695.0 31 70167.5 32 85509.0 33 106469.0 34 125431.0 35 138616.0 36 155525.5 37 169052.5 38 162712.5 39 152489.5 40 140119.5 41 125574.5 42 111534.0 43 105311.0 44 104217.0 45 98162.0 46 89918.0 47 95938.0 48 129867.0 49 118731.5 50 72026.5 51 49339.5 52 34382.0 53 25077.5 54 19194.0 55 15191.5 56 12006.5 57 9314.0 58 7048.0 59 5318.5 60 3972.5 61 3015.0 62 2301.0 63 1753.5 64 1252.0 65 906.0 66 608.0 67 511.5 68 413.5 69 286.0 70 230.0 71 176.5 72 127.5 73 128.5 74 126.5 75 95.0 76 77.5 77 57.0 78 36.0 79 20.5 80 16.5 81 13.0 82 8.0 83 8.0 84 5.5 85 4.5 86 4.5 87 2.5 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2347332063677668 2 0.0 3 0.0 4 0.0 5 0.027958796645346687 6 0.003989324701194551 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0026316133533090106 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.352373698482816E-4 26-27 0.001206854531453814 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006738271133950461 36-37 0.01709710586226236 38-39 0.013325685451469194 40-41 0.008481505457161524 42-43 0.007878078191434618 44-45 0.0037043729368235116 46-47 6.872366081889773E-4 48-49 0.0016929487177338221 50-51 1.676186849241408E-5 52-53 0.0 54-55 0.0 56-57 0.001223616399946228 58-59 2.681898958786253E-4 60-61 0.0024639946683848698 62-63 7.375222136662196E-4 64-65 0.0013577113478855406 66-67 0.005330274180587678 68-69 1.3409494793931265E-4 70-71 1.0057121095448448E-4 72-73 0.0 74-75 5.028560547724225E-4 76-77 0.059655489964501714 78-79 0.13887208045965066 80-81 9.219027670827744E-4 82-83 8.380934246207041E-4 84-85 0.0 86-87 0.10673957855969288 88-89 0.3526529512118999 90-91 0.3880204937308936 92-93 3.687611068331098E-4 94-95 4.022848438179379E-4 96-97 1.1733307944689856E-4 98-99 0.0 100-101 0.0011062833204993293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2982961.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.803363885588467 #Duplication Level Percentage of deduplicated Percentage of total 1 70.5074723422394 16.783150208153888 2 12.98509742132779 6.181779980193637 3 5.428525058636298 3.8765147199826577 4 2.823833324009319 2.6886692865457866 5 1.749279528061508 2.081936857202927 6 1.1679780974444414 1.6681084598320406 7 0.8168572506558249 1.3610765265959366 8 0.595183378387538 1.1333893227529968 9 0.44742339448191626 0.9585163682800423 >10 2.6328416375253973 11.92044387738116 >50 0.34709015934337023 5.861316681377215 >100 0.4140098542321816 20.728267615988365 >500 0.05617826815164007 9.001316876897071 >1k 0.026113014090586466 10.261506615468727 >5k 0.0012703628476501525 1.9156012945563354 >10k+ 8.469085651001016E-4 3.578405308791313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26211 0.8786906701093309 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24199 0.8112409112958567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19251 0.645365460694927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13565 0.454749492199194 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12949 0.4340988702165398 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10329 0.34626667931629007 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8682 0.2910530845022781 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6920 0.2319842599350109 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6617 0.22182656762860792 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6572 0.22031799946429068 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6433 0.21565820002339958 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5717 0.1916552043422626 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5624 0.1885374968026736 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5301 0.17770932975657408 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5148 0.17258019799789537 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4958 0.16621068797077804 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4741 0.15893603704507034 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4504 0.15099091137966605 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4181 0.14016274433356654 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3552 0.11907631377010963 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3491 0.11703136581403512 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3486 0.11686374712911098 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3314 0.11109766436772053 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3301 0.11066185578691777 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3209 0.10757767198431357 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 3045 0.10207977911880176 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 3032 0.10164397053799899 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3011 0.10093997206131759 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.028560547724224E-5 0.0 12-13 0.0 0.0 0.0 1.0057121095448448E-4 0.0 14-15 0.0 0.0 0.0 1.0057121095448448E-4 0.0 16-17 0.0 0.0 0.0 2.01142421908969E-4 0.0 18-19 0.0 0.0 0.0 3.017136328634535E-4 0.0 20-21 0.0 0.0 0.0 4.02284843817938E-4 0.0 22-23 0.0 0.0 0.0 9.721883725600167E-4 0.0 24-25 0.0 0.0 0.0 0.0024807565368772837 0.0 26-27 0.0 0.0 0.0 0.0046262757039062866 0.0 28-29 0.0 0.0 0.0 0.012521115763833319 0.0 30-31 0.0 0.0 0.0 0.039474200299635165 0.0 32-33 0.0 0.0 0.0 0.09379941608354919 0.0 34-35 1.676186849241408E-5 0.0 0.0 0.17065258312126774 0.0 36-37 5.028560547724225E-5 0.0 0.0 0.2818005330944655 0.0 38-39 6.704747396965633E-5 0.0 0.0 0.45751520050044236 0.0 40-41 6.704747396965633E-5 0.0 0.0 0.6694857894555108 0.0 42-43 6.704747396965633E-5 0.0 0.0 0.8721200176603046 0.0 44-45 6.704747396965633E-5 0.0 0.0 1.1113621666525306 0.0 46-47 6.704747396965633E-5 0.0 0.0 1.4084662856805705 0.0 48-49 6.704747396965633E-5 0.0 0.0 1.7226507487023799 0.0 50-51 6.704747396965633E-5 0.0 0.0 2.076728458736135 0.0 52-53 6.704747396965633E-5 0.0 0.0 2.41233458969125 0.0 54-55 6.704747396965633E-5 0.0 0.0 2.7651719214565658 0.0 56-57 1.0057121095448448E-4 0.0 0.0 3.1894148129995665 0.0 58-59 1.0057121095448448E-4 0.0 0.0 3.6540202838723 0.0 60-61 1.0057121095448448E-4 0.0 0.0 4.182488473701131 0.0 62-63 1.1733307944689856E-4 0.0 0.0 4.700061449009893 0.0 64-65 1.5085681643172674E-4 0.0 0.0 5.219327373036389 0.0 66-67 1.676186849241408E-4 0.0 0.0 5.792046895685194 0.0 68-69 1.676186849241408E-4 0.0 0.0 6.4236508623478485 0.0 70-71 2.0114242190896896E-4 0.0 0.0 7.137019223516499 0.0 72-73 2.0114242190896896E-4 0.0 0.0 7.833072574532486 0.0 74-75 2.3466615889379715E-4 0.0 0.0 8.545099986221743 0.0 76-77 3.017136328634535E-4 0.0 0.0 9.265977664474995 0.0 78-79 3.017136328634535E-4 0.0 0.0 10.09357815941945 0.0 80-81 3.017136328634535E-4 0.0 0.0 10.998048583270112 0.0 82-83 3.017136328634535E-4 0.0 0.0 11.867704606262032 0.0 84-85 3.017136328634535E-4 0.0 0.0 12.73234883057472 0.0 86-87 3.017136328634535E-4 0.0 0.0 13.615364062755095 0.0 88-89 3.017136328634535E-4 0.0 0.0 14.541222630802078 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTCCG 40 5.419005E-4 47.44755 9 CTTAACG 45 5.7521174E-7 36.90427 20-21 TCACGCA 90 2.4269755E-5 31.64231 4 ATATCGC 60 0.0039733844 31.631699 9 TAGCCCG 65 0.0058675683 29.208282 5 GTAAGCG 145 0.0 27.814314 94-95 CATGGGT 2885 0.0 26.48741 4 ATCACGC 110 9.53133E-5 25.88916 3 TACGCGC 55 9.5459436E-5 25.883951 64-65 CATTCCG 770 0.0 25.264282 9 CTTTGCG 420 0.0 24.861814 2 CACGCAG 135 1.3516974E-5 24.610687 5 GTACATG 22780 0.0 24.53983 1 TATCACG 120 1.7206612E-4 23.73173 2 CATGGGG 7770 0.0 23.456846 4 ACATGGG 21940 0.0 23.190899 3 TACATGG 23780 0.0 23.033152 2 GAGTACT 20800 0.0 23.029188 12-13 GACAATG 1385 0.0 22.267805 7 AGTACTT 22295 0.0 21.782915 12-13 >>END_MODULE