##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938779_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 986993 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.630402647232554 33.0 31.0 34.0 30.0 34.0 2 31.59431728492502 33.0 31.0 34.0 30.0 34.0 3 31.59752906048979 33.0 31.0 34.0 28.0 34.0 4 35.15922098738289 37.0 35.0 37.0 33.0 37.0 5 35.25018718471154 37.0 35.0 37.0 33.0 37.0 6 35.40679923768457 37.0 35.0 37.0 33.0 37.0 7 35.35353138269471 37.0 35.0 37.0 33.0 37.0 8 35.348224354174754 37.0 35.0 37.0 33.0 37.0 9 36.82058028780346 39.0 37.0 39.0 33.0 39.0 10-11 36.744887755029666 39.0 37.0 39.0 32.5 39.0 12-13 36.652936748284944 39.0 37.0 39.0 32.0 39.0 14-15 37.61835747568625 40.0 37.0 41.0 32.0 41.0 16-17 37.640034934391636 40.0 37.0 41.0 32.5 41.0 18-19 37.72777213212252 40.0 37.0 41.0 33.0 41.0 20-21 37.65524628847419 40.0 37.0 41.0 32.0 41.0 22-23 37.54953125300787 40.0 37.0 41.0 32.0 41.0 24-25 37.47157629284098 40.0 36.5 41.0 32.0 41.0 26-27 37.27858454923186 39.5 36.0 41.0 32.0 41.0 28-29 37.11802819270248 39.0 36.0 41.0 32.0 41.0 30-31 36.841120960331025 39.0 35.0 41.0 31.0 41.0 32-33 36.47306769146286 38.5 35.0 41.0 31.0 41.0 34-35 36.060328188751086 38.0 35.0 40.5 30.0 41.0 36-37 35.70434795383554 38.0 35.0 40.0 30.0 41.0 38-39 35.45939839492276 38.0 35.0 40.0 29.5 41.0 40-41 35.30240234733174 38.0 35.0 40.0 28.5 41.0 42-43 34.99726340511027 38.0 35.0 40.0 26.5 41.0 44-45 34.7324869578609 38.0 35.0 40.0 24.0 41.0 46-47 34.37342666057408 38.0 34.5 40.0 23.0 41.0 48-49 34.03336143214795 38.0 34.0 40.0 20.5 41.0 50-51 32.84981605745938 36.5 32.5 39.0 17.5 40.5 52-53 33.05408245043278 37.5 33.0 39.5 19.0 40.5 54-55 33.63352222356187 38.0 34.0 40.0 18.5 41.0 56-57 33.550246050377254 38.0 34.0 40.0 14.0 41.0 58-59 33.36518546737413 38.0 34.0 40.0 11.0 41.0 60-61 33.014891189704485 37.0 33.0 40.0 7.0 41.0 62-63 32.65324120839763 36.5 33.0 40.0 2.0 41.0 64-65 32.21718593748892 36.0 32.5 39.5 2.0 41.0 66-67 31.729968196329658 36.0 32.0 39.0 2.0 41.0 68-69 31.275387464754054 35.0 31.0 39.0 2.0 40.0 70-71 30.730103962236814 35.0 31.0 37.5 2.0 40.0 72-73 30.225118111273332 35.0 30.5 37.0 2.0 39.5 74-75 29.7449688093026 35.0 30.0 37.0 2.0 39.0 76-77 29.23353863705214 35.0 29.0 36.0 2.0 39.0 78-79 28.741941938797943 34.5 29.0 36.0 2.0 37.5 80-81 28.213734038640595 34.0 28.0 35.0 2.0 37.0 82-83 27.66242415093116 34.0 26.5 35.0 2.0 37.0 84-85 27.16031623324583 34.0 26.0 35.0 2.0 36.0 86-87 26.76824708989831 34.0 25.0 35.0 2.0 36.0 88-89 26.283812549835712 34.0 24.0 35.0 2.0 35.5 90-91 25.733756470410633 33.0 20.0 35.0 2.0 35.0 92-93 25.194099147613002 33.0 15.0 35.0 2.0 35.0 94-95 24.60009341504955 33.0 2.0 35.0 2.0 35.0 96-97 23.939553775963965 32.5 2.0 35.0 2.0 35.0 98-99 23.272475083409912 32.0 2.0 35.0 2.0 35.0 100-101 21.320391330029697 29.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11800559578064096 1101 2 0.03255561421211439 1101 3 -0.03047883605834656 1101 4 0.019577908930990873 1101 5 -0.026226423439339897 1101 6 -0.16414385065978365 1101 7 -0.014843336034580545 1101 8 0.11648285004444858 1101 9 0.09356352404790869 1101 10-11 0.11120662283005345 1101 12-13 0.22666187822708395 1101 14-15 0.21978938740179643 1101 16-17 0.26370037706952587 1101 18-19 0.18161369540507621 1101 20-21 0.17933952410432852 1101 22-23 0.2862461728984087 1101 24-25 0.20015011509708103 1101 26-27 0.29443921776869075 1101 28-29 0.06347696234793432 1101 30-31 0.08202350977476414 1101 32-33 0.009392608134007219 1101 34-35 -0.06420180031003042 1101 36-37 -0.14122185011964206 1101 38-39 0.044467509122057436 1101 40-41 -0.07389751732720384 1101 42-43 -0.1494134880112412 1101 44-45 -0.031116256745022497 1101 46-47 -0.3770429665119863 1101 48-49 -0.28728014007727154 1101 50-51 -0.3128493017730989 1101 52-53 -0.2770841562337196 1101 54-55 -0.40354627067163307 1101 56-57 -0.28830534948190945 1101 58-59 -0.45660915432461735 1101 60-61 -0.7336263708352959 1101 62-63 -0.673322811786953 1101 64-65 -0.6377001289323516 1101 66-67 -0.7543371958048084 1101 68-69 -0.670629388939119 1101 70-71 -0.6404798584412283 1101 72-73 -0.9304817106643846 1101 74-75 -0.7338613638805356 1101 76-77 -0.7901684323112583 1101 78-79 -0.7998081031239295 1101 80-81 -0.9121098531555454 1101 82-83 -1.166272808016771 1101 84-85 -1.080215262068716 1101 86-87 -0.8114991523418489 1101 88-89 -0.7851143119034596 1101 90-91 -0.6498613761971441 1101 92-93 -0.7621712672977132 1101 94-95 -0.6895426580619777 1101 96-97 -0.7848478640895564 1101 98-99 -0.6601850489719645 1101 100-101 -0.39162326356522925 1102 1 0.2821514534708136 1102 2 0.22963469824410865 1102 3 0.10978442021410828 1102 4 0.1687894021055243 1102 5 0.0172813425730709 1102 6 0.17421760763138394 1102 7 0.30149636361134924 1102 8 0.21778151114247635 1102 9 0.30325776484072264 1102 10-11 0.21481437193654784 1102 12-13 0.3045589234992576 1102 14-15 0.2193738825217011 1102 16-17 0.47468875483846773 1102 18-19 0.3332688096867784 1102 20-21 0.2746175313953074 1102 22-23 0.2441390988385308 1102 24-25 0.3654734350405988 1102 26-27 0.34335390473516014 1102 28-29 0.42285832439435467 1102 30-31 0.48578888881284144 1102 32-33 0.6300176036694154 1102 34-35 0.6130961559774235 1102 36-37 0.49373508738318606 1102 38-39 0.5373170153974911 1102 40-41 0.5221793546472924 1102 42-43 0.5225174310284117 1102 44-45 0.9499550553217588 1102 46-47 0.840293468622825 1102 48-49 0.9682785770722404 1102 50-51 0.8828070649760278 1102 52-53 0.919618116066026 1102 54-55 0.8771612067494274 1102 56-57 0.8561081933579544 1102 58-59 0.7830163198152604 1102 60-61 0.8784575587693624 1102 62-63 0.799691991616136 1102 64-65 0.86844493822705 1102 66-67 0.8208197793196668 1102 68-69 0.6660629064812795 1102 70-71 0.6346653301551264 1102 72-73 0.7901537617849002 1102 74-75 0.6822971327775704 1102 76-77 0.6291087320643776 1102 78-79 0.5238541259347471 1102 80-81 0.44368911867822547 1102 82-83 0.4975670761657085 1102 84-85 0.1518942169191213 1102 86-87 -0.0328334825694121 1102 88-89 -0.1223262860635792 1102 90-91 0.027115724602673197 1102 92-93 -0.0194086717851647 1102 94-95 0.14376547511864501 1102 96-97 0.21106554421720958 1102 98-99 0.16367659861798955 1102 100-101 0.6102810345886915 1103 1 0.20108826503235377 1103 2 0.20829251909281865 1103 3 0.14821020267459772 1103 4 0.32166851865058277 1103 5 0.15947645765321994 1103 6 0.2713737053297791 1103 7 0.06294568796739952 1103 8 0.23377582052073365 1103 9 0.4316582869505723 1103 10-11 0.2348137146040017 1103 12-13 0.12760382870251163 1103 14-15 0.3949192337720362 1103 16-17 0.34554873361602034 1103 18-19 0.48884885870501193 1103 20-21 0.5295463516712005 1103 22-23 0.3180549696633008 1103 24-25 0.38463115728942654 1103 26-27 0.2850719841972804 1103 28-29 0.38151562876749523 1103 30-31 0.33561774758964447 1103 32-33 0.19138898716661146 1103 34-35 0.3923697565633901 1103 36-37 0.43198691148099755 1103 38-39 0.3260785069789378 1103 40-41 0.43309553535739553 1103 42-43 0.24853653123405905 1103 44-45 0.38556228973592965 1103 46-47 0.46686637149724675 1103 48-49 0.5785331995286072 1103 50-51 0.6884432220221655 1103 52-53 0.7620907193846236 1103 54-55 0.7920519017377359 1103 56-57 0.9455042456065001 1103 58-59 0.9119121889497492 1103 60-61 0.7411313997512323 1103 62-63 0.7764071600341254 1103 64-65 0.5029093530602253 1103 66-67 0.43154744644646215 1103 68-69 0.38093435101649575 1103 70-71 0.6040488930853272 1103 72-73 0.5411374129854742 1103 74-75 0.3988694252717693 1103 76-77 0.652123587779883 1103 78-79 0.5508329316554139 1103 80-81 0.49959877301520805 1103 82-83 0.6665966185683487 1103 84-85 0.6285671282449599 1103 86-87 0.6128501902292278 1103 88-89 0.45959026087465915 1103 90-91 0.4805742328334155 1103 92-93 0.29191693319553735 1103 94-95 0.14113731874862268 1103 96-97 0.24329244602111544 1103 98-99 -0.03471223236303089 1103 100-101 0.20792661027114434 1104 1 0.08796774069687885 1104 2 0.008177213837324615 1104 3 -0.11725486185588707 1104 4 -0.14290714452321396 1104 5 -0.1380043585191828 1104 6 -0.04921873442153668 1104 7 -0.1607330216926215 1104 8 0.09313375230856735 1104 9 0.02564892767018989 1104 10-11 -0.03951839150380465 1104 12-13 -0.050200583914978836 1104 14-15 -0.2285180765811603 1104 16-17 -0.03379544245713362 1104 18-19 0.1214279877794624 1104 20-21 0.08262554143263401 1104 22-23 0.23127765040934634 1104 24-25 0.10497039748847925 1104 26-27 0.22993112298165386 1104 28-29 0.2368631628533464 1104 30-31 0.4042294318555335 1104 32-33 0.14610975964438921 1104 34-35 0.14814344808474544 1104 36-37 0.11638934705165838 1104 38-39 0.16475229425085303 1104 40-41 -0.00655969518568611 1104 42-43 0.07158679353666031 1104 44-45 0.18803629548096978 1104 46-47 0.18691229130951825 1104 48-49 0.0663502889812122 1104 50-51 0.06750082070593777 1104 52-53 0.24865866856939078 1104 54-55 0.36481390090160204 1104 56-57 0.3011653290713241 1104 58-59 0.4235101444729352 1104 60-61 0.532941355112591 1104 62-63 0.3803656366561583 1104 64-65 0.4642652349768568 1104 66-67 0.3303249410183504 1104 68-69 0.3304916732373684 1104 70-71 0.25700536029413357 1104 72-73 0.10700975455874939 1104 74-75 0.09902856001034621 1104 76-77 0.09374777356294572 1104 78-79 0.11604783098246685 1104 80-81 0.024728238565238314 1104 82-83 0.062995520808375 1104 84-85 -0.04630070900605432 1104 86-87 -0.11378869528816438 1104 88-89 -0.2960924001818377 1104 90-91 -0.2515332235731087 1104 92-93 -0.05717647080440713 1104 94-95 -0.23399416970061893 1104 96-97 -0.3056094568527499 1104 98-99 -0.2523475306058067 1104 100-101 0.195538038303134 1105 1 0.05900978676544 1105 2 0.11365927938463116 1105 3 0.1290912060395435 1105 4 0.08694981123380074 1105 5 0.21463650103596166 1105 6 0.04534545092893438 1105 7 0.15211205336503042 1105 8 0.07610319336202309 1105 9 0.14999491106878082 1105 10-11 0.07549569500760356 1105 12-13 0.04541647644021651 1105 14-15 0.21961309808046536 1105 16-17 0.05985762244310422 1105 18-19 0.07634781585063877 1105 20-21 0.0731457750886122 1105 22-23 0.009438443434355293 1105 24-25 0.0145209855197308 1105 26-27 -0.07861072852183071 1105 28-29 0.0066871203736766915 1105 30-31 0.05800644026741253 1105 32-33 0.04515994853928618 1105 34-35 0.27775658600803865 1105 36-37 0.22143113962972194 1105 38-39 0.29576852431349465 1105 40-41 0.07262829025417261 1105 42-43 0.2217143170456808 1105 44-45 0.2963217658059065 1105 46-47 0.44491892208726824 1105 48-49 0.3207963788902788 1105 50-51 0.20876729480876577 1105 52-53 0.29881309764875397 1105 54-55 0.3874974091211456 1105 56-57 0.5585832615227702 1105 58-59 0.4769121426006606 1105 60-61 0.8695322636981402 1105 62-63 0.6662968028680964 1105 64-65 0.5889703299134936 1105 66-67 0.5225052038924396 1105 68-69 0.6160064701892551 1105 70-71 0.3158885345288205 1105 72-73 0.44588541302254825 1105 74-75 0.4132144663834083 1105 76-77 0.5392739998232301 1105 78-79 0.5897754309833587 1105 80-81 0.4441849684044037 1105 82-83 0.4767959659732668 1105 84-85 0.3116100845748555 1105 86-87 0.4159569692460714 1105 88-89 0.19260448077324455 1105 90-91 0.44464674133847026 1105 92-93 0.46344909482405683 1105 94-95 0.465424469855904 1105 96-97 0.5768749056742415 1105 98-99 0.5653171993045127 1105 100-101 0.5662200833933113 1106 1 -0.12977458939070985 1106 2 0.08577829684037752 1106 3 0.005427854133088772 1106 4 0.01150669094948853 1106 5 -0.08438700872333271 1106 6 -0.004063161468344845 1106 7 -0.036140914784127176 1106 8 0.05950846483976591 1106 9 -0.061041334544860604 1106 10-11 -7.24917793270663E-4 1106 12-13 -0.12749068411451248 1106 14-15 0.038603647089956894 1106 16-17 0.021946594045289203 1106 18-19 -0.12277995750496018 1106 20-21 -0.03334603517406265 1106 22-23 0.11107246700016304 1106 24-25 0.09566048572713015 1106 26-27 -0.08472584450107234 1106 28-29 0.20577901808324128 1106 30-31 0.14337085835322938 1106 32-33 0.022340234871784048 1106 34-35 0.2524210926618551 1106 36-37 0.2595333179541015 1106 38-39 0.45036172812080366 1106 40-41 0.2620517013554462 1106 42-43 0.44753020771464946 1106 44-45 0.1900261504825167 1106 46-47 0.3245662196421151 1106 48-49 0.14989320950117957 1106 50-51 0.3893294213376848 1106 52-53 0.1940550900924478 1106 54-55 0.2647774745942044 1106 56-57 0.26991155731747085 1106 58-59 0.11484503272028945 1106 60-61 0.3536673803500392 1106 62-63 0.03189037664299832 1106 64-65 0.20330825607693015 1106 66-67 0.07202816991147998 1106 68-69 0.5259372974592331 1106 70-71 0.43411224160417206 1106 72-73 0.2739199881764236 1106 74-75 0.42141877818761486 1106 76-77 0.12165793650271439 1106 78-79 0.4045545154817134 1106 80-81 0.30222167367475805 1106 82-83 0.12141461906698225 1106 84-85 0.2643080452685993 1106 86-87 0.3463217797217588 1106 88-89 0.3985110695598628 1106 90-91 0.4424857942214153 1106 92-93 0.38579569840938177 1106 94-95 0.4300807923779608 1106 96-97 0.39937061741342106 1106 98-99 0.6370942770089236 1106 100-101 0.914160036530749 1107 1 0.01800502702683815 1107 2 0.07213860392336002 1107 3 -0.004343461278029537 1107 4 -0.012457717807258462 1107 5 0.00883383602614174 1107 6 0.08986842304729947 1107 7 -0.06487433311686885 1107 8 0.004043635607203555 1107 9 -0.16896526911340004 1107 10-11 0.027826746477131792 1107 12-13 0.07643466108257257 1107 14-15 0.10422771458311075 1107 16-17 0.08505594488488555 1107 18-19 0.24700275144439132 1107 20-21 0.09758742113713481 1107 22-23 0.11088800019062717 1107 24-25 0.11608204664187127 1107 26-27 0.33550039861533065 1107 28-29 0.025641753074964413 1107 30-31 0.32019314628421824 1107 32-33 0.36701646849357417 1107 34-35 0.32635973032020615 1107 36-37 0.6103783387066173 1107 38-39 0.3870950379436593 1107 40-41 0.31700924686177956 1107 42-43 0.4179531250029882 1107 44-45 0.3725180800596064 1107 46-47 0.23485024427386492 1107 48-49 0.46653591356758994 1107 50-51 0.6395613612232083 1107 52-53 0.5961059269749569 1107 54-55 0.45381255041548485 1107 56-57 0.5319547985078259 1107 58-59 0.4865348029505938 1107 60-61 0.5612332322882949 1107 62-63 0.42836923077857847 1107 64-65 0.6777921559223046 1107 66-67 0.5591263442326131 1107 68-69 0.43122986724465306 1107 70-71 0.571995756239442 1107 72-73 0.6300928444279492 1107 74-75 0.6464682858239996 1107 76-77 0.4272570467216319 1107 78-79 0.4313266175895194 1107 80-81 0.47291315293580993 1107 82-83 0.4749669615241885 1107 84-85 0.34887251370578554 1107 86-87 0.30462956801896723 1107 88-89 0.4169007828224842 1107 90-91 0.5340138847186644 1107 92-93 0.3337462179004689 1107 94-95 0.4337023098887478 1107 96-97 0.29937604290782005 1107 98-99 0.4103682916035005 1107 100-101 0.5904126475499254 1108 1 -0.04340579634878594 1108 2 0.013495031060333673 1108 3 -0.08714803014039774 1108 4 -0.04744736038391295 1108 5 -0.03018281491551278 1108 6 -0.11514847035821418 1108 7 -0.04472453262245324 1108 8 0.01712192507741861 1108 9 -0.1025575819550184 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-1.7504573125810374 2316 88-89 -1.869423037061292 2316 90-91 -2.0505599184664796 2316 92-93 -1.9954506255808617 2316 94-95 -1.8430026205018102 2316 96-97 -1.8655754356368242 2316 98-99 -2.377782835792754 2316 100-101 -2.6301333500263837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4799.0 3 1040.0 4 868.0 5 865.0 6 1067.0 7 1295.0 8 1600.0 9 2058.0 10 2619.0 11 3796.0 12 5674.0 13 7812.0 14 8511.0 15 9313.0 16 11573.0 17 14421.0 18 11992.0 19 8434.0 20 8735.0 21 9135.0 22 9885.0 23 11377.0 24 12349.0 25 13637.0 26 15492.0 27 17731.0 28 20244.0 29 23257.0 30 27215.0 31 32228.0 32 38967.0 33 48015.0 34 64733.0 35 108855.0 36 111749.0 37 151352.0 38 142001.0 39 22297.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.11331606890617 20.78276452728483 14.782699582731244 17.321219821077754 2 13.621695734802863 26.091246701566007 38.62620858069475 21.660848982936372 3 22.296259264629978 31.055130949568166 30.810845891518774 15.83776389428308 4 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19.19430823179479 24-25 19.00568518839601 30.465289121285032 33.689937713372004 16.83908797694695 26-27 18.42432667563013 28.565612750018758 36.780819000918655 16.22924157343246 28-29 15.998495423410564 32.03151297139682 35.85221489084723 16.11777671434539 30-31 16.54261789870646 31.404849762783343 34.49414054580106 17.558391792709134 32-33 16.553138693678214 32.007590155908375 34.56394615124967 16.875324999163745 34-35 18.261144341743897 30.303170395418 34.23591163982081 17.199773623017297 36-37 17.95135499879395 29.82457836622574 35.71783334537687 16.50623328960345 38-39 17.429427339312497 31.124573389258263 35.41449163873473 16.03150763269451 40-41 16.207508685191684 32.01150565364015 35.444890360581695 16.33609530058647 42-43 15.439686379859928 32.921414343389635 33.07858056867818 18.56031870807226 44-45 16.450647091928065 31.42240137916015 32.92078275886984 19.20616877004194 46-47 17.730688442374703 32.30823794248868 32.45559847917413 17.505475135962485 48-49 17.432781525708727 31.67609530284956 32.8936922118583 17.997430959583415 50-51 15.658127208480565 32.2854415332758 34.55269617820911 17.50373508003452 52-53 16.307872537923508 33.38653074185957 32.52968944655696 17.775907273659953 54-55 15.12700356862803 31.276050399428858 33.62250092993978 19.974445102003326 56-57 17.293716209100516 31.62189703705927 31.917687442033678 19.166699311806536 58-59 18.463678697046536 30.52449268690917 32.15343884394416 18.858389772100132 60-61 16.971015966642938 31.013932675683918 34.03986575816129 17.975185599511846 62-63 17.159643796740482 30.838455421425614 33.65793151588878 18.343969265945123 64-65 16.48038323489832 32.29839144765639 33.20721028854591 18.01401502889938 66-67 16.66042322397808 30.680939040925765 32.19514355167738 20.463494183418774 68-69 15.726225375040531 31.265942181325624 32.849534313962536 20.158298129671305 70-71 17.663260501635214 30.730819642925315 31.77292798696919 19.83299186847028 72-73 16.88212803902328 28.88766963765844 33.522752720920856 20.707449602397425 74-75 15.786741527058346 31.925358109462966 32.35148623188332 19.936414131595367 76-77 16.7397706942067 29.24600404226261 32.88515984848639 21.129065415044302 78-79 16.373757018360035 29.387400247337258 33.72892711486837 20.50991561943434 80-81 17.316098363057687 29.826832911413014 32.495868979495484 20.361199746033815 82-83 16.707413062624624 28.843869138688184 33.49781139701711 20.950906401670085 84-85 16.247421694299184 28.96733450762629 33.086314602648365 21.69892919542616 86-87 16.66022124749392 29.288680147769714 32.87525060807441 21.17584799666195 88-89 17.021092524258158 28.364908252328615 32.401072972184856 22.212926251228364 90-91 16.879098397156152 27.205138057299315 33.10464160574469 22.811121939799843 92-93 17.753169018206172 28.648423341237113 32.67451548005657 20.92389216050014 94-95 17.148042644131596 27.800372451610045 32.59867702826571 22.452907875992647 96-97 16.021258121683505 27.25251704751929 33.297146480917675 23.42907834987953 98-99 17.485797052179006 27.72866326125027 31.58344737593446 23.202092310636267 100-101 17.550722152366692 26.133097085387654 32.5011055040106 23.815075258235048 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12267.0 1 7939.0 2 4870.5 3 5776.0 4 4288.5 5 4402.5 6 8341.5 7 14161.0 8 18611.0 9 17710.0 10 12337.5 11 8318.5 12 7153.5 13 6519.0 14 5693.0 15 4763.5 16 4197.5 17 3799.0 18 3379.5 19 3153.0 20 3105.5 21 3262.5 22 3445.5 23 3694.0 24 4017.0 25 4590.0 26 5871.0 27 7489.0 28 8146.0 29 8989.5 30 11370.5 31 13632.0 32 17013.5 33 21446.5 34 25156.5 35 28136.5 36 32197.0 37 35491.0 38 34834.0 39 32819.5 40 30167.0 41 27552.0 42 24726.5 43 24074.0 44 24571.0 45 24463.0 46 29197.0 47 43069.0 48 75955.0 49 77540.5 50 44827.0 51 25837.0 52 15140.0 53 11087.0 54 9031.5 55 7433.0 56 6143.5 57 5167.5 58 4472.5 59 3844.0 60 3288.0 61 2727.0 62 2222.5 63 1738.5 64 1270.5 65 998.0 66 812.0 67 665.5 68 538.0 69 414.0 70 330.5 71 261.0 72 215.5 73 207.5 74 187.5 75 147.5 76 98.5 77 72.0 78 65.5 79 47.5 80 32.5 81 25.0 82 18.5 83 17.5 84 11.5 85 8.5 86 7.5 87 4.5 88 1.0 89 0.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15572552186287034 2 0.09311109602601032 3 0.08612016498597254 4 0.10000070922488813 5 0.11722474222208262 6 0.03556256224714866 7 0.03799419043498788 8 0.04184426839906666 9 0.0423508576048665 10-11 0.041388338113846804 12-13 0.04518775715734559 14-15 0.04280678789008635 16-17 0.04133767919326682 18-19 0.04539039283966553 20-21 0.047062137218805 22-23 0.059372254919741074 24-25 0.07558310950533591 26-27 0.07771078416969522 28-29 0.06778163573601839 30-31 0.05602876616146214 32-33 0.0457956642043054 34-35 0.28308204820094973 36-37 0.4504591217972164 38-39 0.4302968714063828 40-41 0.40506872895755086 42-43 0.43318442987944195 44-45 0.442353694504419 46-47 0.4426576480278989 48-49 0.45846323124885385 50-51 0.44752090440357734 52-53 0.454815788967095 54-55 0.4460517957067578 56-57 0.42578822747476425 58-59 0.404815434354651 60-61 0.37416678740376075 62-63 0.42953698759768305 64-65 0.46798710831789075 66-67 0.46900028672949046 68-69 0.47948668328954713 70-71 0.47675110157822803 72-73 0.46656865854165125 74-75 0.46018563454857325 76-77 0.4432655550748587 78-79 0.41788543586428684 80-81 0.4240151652544648 82-83 0.43450156181452143 84-85 0.433032453117702 86-87 0.4440760978041384 88-89 0.454765130046515 90-91 0.458007300963634 92-93 0.461148154039593 94-95 0.4643903249567119 96-97 0.46525152660657165 98-99 0.4598310221045134 100-101 0.44676102059487754 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 986993.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.964635783622015 #Duplication Level Percentage of deduplicated Percentage of total 1 81.97843404314558 25.38432352257683 2 8.610108852448302 5.332177693468027 3 2.723374718703991 2.5298491880097944 4 1.3218927815339119 1.6372771410078641 5 0.8418278804386177 1.303344685514015 6 0.575873240564029 1.0699023090959578 7 0.4316826358836987 0.9356826915976854 8 0.3300281371995554 0.8175360853385174 9 0.27618447531342466 0.7696756518453844 >10 2.3163419625878365 14.782701478029193 >50 0.3490775573522585 7.675244090984116 >100 0.21731306541644754 12.203478527275992 >500 0.011144259764945996 2.2338722243643425 >1k 0.012455349149057288 9.583220498361197 >5k 0.0022944064221947637 4.939181817296261 >10k+ 0.0019666340761669405 8.802532395234866 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19972 2.023519923646875 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18616 1.8861329310339587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13201 1.3374968211527336 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12946 1.311660771656942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11529 1.1680933907332676 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10466 1.060392525580222 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8867 0.8983852975654336 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7843 0.7946358282176267 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7604 0.7704208641803943 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6762 0.6851112419237017 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6584 0.6670766661972274 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5576 0.5649482823079799 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5429 0.5500545596574646 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4753 0.4815636990333265 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4671 0.4732556360582091 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4670 0.4731543182170492 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4575 0.46352912330685225 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4302 0.435869352670181 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4063 0.41165438863294873 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4046 0.40993198533322933 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3905 0.3956461697296739 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3750 0.3799419043498789 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3183 0.32249468841217716 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3041 0.3081075549674618 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3004 0.304358794844543 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2806 0.28429786229486936 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2690 0.27254499272031313 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2645 0.26798568986811455 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2455 0.2487353000477207 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2362 0.2393127408198437 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2353 0.23840088024940398 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2344 0.2374890196789643 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2046 0.20729630301329394 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2023 0.20496599266661467 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1995 0.20212909311413557 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1838 0.18622219205202065 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1832 0.18561428500506083 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1793 0.18166288919982207 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1757 0.17801544691806323 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1621 0.16423622052030765 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1533 0.1553202504982305 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1476 0.14954513355211232 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1393 0.141135752735835 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1362 0.13799489965987602 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1343 0.13606986067783663 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1253 0.12695125497343954 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1187 0.12026427745688165 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1168 0.11833923847484228 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1096 0.1110443539113246 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1053 0.10668768674144599 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1035 0.10486396560056657 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5197676173995153E-4 0.0 12-13 0.0 0.0 0.0 2.026356823199354E-4 0.0 14-15 0.0 0.0 0.0 2.5329460289991927E-4 0.0 16-17 0.0 0.0 0.0 5.065892057998385E-4 0.0 18-19 0.0 0.0 0.0 8.612016498597255E-4 0.0 20-21 0.0 0.0 0.0 0.002279651426099273 0.0 22-23 0.0 0.0 0.0 0.006484341834237933 0.0 24-25 0.0 0.0 0.0 0.01327263719195577 0.0 26-27 0.0 0.0 0.0 0.02264453749925278 0.0 28-29 0.0 0.0 0.0 0.05076023842114382 0.0 30-31 2.026356823199354E-4 0.0 0.0 0.11580629244584309 0.0 32-33 2.026356823199354E-4 0.0 0.0 0.2377929732024442 0.0 34-35 2.026356823199354E-4 0.0 0.0 0.4303475303269628 0.0 36-37 2.026356823199354E-4 0.0 0.0 0.7016767089533563 0.0 38-39 2.026356823199354E-4 0.0 0.0 1.0657117122411202 0.0 40-41 2.026356823199354E-4 0.0 0.0 1.4562413309922158 0.0 42-43 2.026356823199354E-4 0.0 0.0 1.8437820734290922 0.0 44-45 3.039535234799031E-4 0.0 0.0 2.2807659223520327 0.0 46-47 3.039535234799031E-4 0.0 0.0 2.816534666405942 0.0 48-49 3.039535234799031E-4 0.0 0.0 3.335079377462657 0.0 50-51 3.039535234799031E-4 0.0 0.0 3.891972891398419 0.0 52-53 3.039535234799031E-4 0.0 0.0 4.381236746359903 0.0 54-55 3.039535234799031E-4 0.0 0.0 4.896792581102399 0.0 56-57 3.039535234799031E-4 0.0 0.0 5.541731299006173 0.0 58-59 3.039535234799031E-4 0.0 0.0 6.199233429213784 0.0 60-61 3.039535234799031E-4 0.0 0.0 6.972896464311297 0.0 62-63 3.039535234799031E-4 0.0 0.0 7.678524569069892 0.0 64-65 3.039535234799031E-4 0.0 0.0 8.316573673774787 0.0 66-67 3.039535234799031E-4 0.0 0.0 9.041654804036098 0.0 68-69 3.039535234799031E-4 0.0 0.0 9.857364743214998 0.0 70-71 4.052713646398708E-4 0.0 0.0 10.742376085747317 0.0 72-73 4.052713646398708E-4 0.0 0.0 11.568927033930333 0.0 74-75 4.052713646398708E-4 0.0 0.0 12.449581709292772 0.0 76-77 4.052713646398708E-4 0.0 0.0 13.2399115292611 0.0 78-79 4.052713646398708E-4 0.0 0.0 14.248429320167418 0.0 80-81 4.052713646398708E-4 0.0 0.0 15.334657895243431 0.0 82-83 4.052713646398708E-4 0.0 0.0 16.28527253992683 0.0 84-85 4.052713646398708E-4 0.0 0.0 17.372210339890962 0.0 86-87 4.052713646398708E-4 0.0 0.0 18.47196484676183 0.0 88-89 4.052713646398708E-4 0.0 0.0 19.499986322091445 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGCCG 30 0.0039296583 31.702753 94-95 ACACGGA 165 8.0035534E-11 31.570988 6 CATGGGG 1235 0.0 31.46869 4 GGGATAC 65 0.0059277853 29.145403 7 GTATGGT 100 5.0051734E-5 28.455715 1 GTACATG 10210 0.0 27.080774 1 ATGGGAT 2255 0.0 26.482306 5 TACATGG 10020 0.0 26.109776 2 GGGATGC 200 7.421477E-10 26.048704 9 ATATAGG 460 0.0 25.758106 2 ACATGGG 9795 0.0 25.403095 3 ATGGGGG 635 0.0 25.376804 5 CTACACG 190 1.2189048E-8 24.944695 4 CACGGAG 190 1.22818165E-8 24.92699 7 AGGCTTA 390 0.0 24.285376 6 CATGGGA 7390 0.0 23.79366 4 CCTTTAG 200 2.0874722E-8 23.713097 1 CCCTATA 140 1.8099036E-5 23.697458 2 ACGGAGG 180 2.006309E-7 23.680641 8 GAGTACT 9570 0.0 23.581661 12-13 >>END_MODULE