##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938779_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 986993 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75778855574457 31.0 31.0 34.0 30.0 34.0 2 31.922168647599324 31.0 31.0 34.0 30.0 34.0 3 31.995370787837402 33.0 31.0 34.0 30.0 34.0 4 35.67015470221167 37.0 35.0 37.0 33.0 37.0 5 35.60764362057279 37.0 35.0 37.0 33.0 37.0 6 35.69221868848107 37.0 35.0 37.0 33.0 37.0 7 35.618615329592004 37.0 35.0 37.0 33.0 37.0 8 35.63460429810546 37.0 35.0 37.0 33.0 37.0 9 37.15837397023079 39.0 37.0 39.0 33.0 39.0 10-11 37.090423133700035 39.0 37.0 39.0 33.0 39.0 12-13 37.0425985797265 39.0 37.0 39.0 33.0 39.0 14-15 37.9901914197973 40.0 37.0 41.0 33.0 41.0 16-17 37.9908727822791 40.0 37.0 41.0 33.0 41.0 18-19 38.12359560807422 40.0 37.0 41.0 33.0 41.0 20-21 38.18707427509618 40.0 37.0 41.0 33.0 41.0 22-23 38.08841552067745 40.0 37.0 41.0 33.0 41.0 24-25 37.94082531487052 40.0 37.0 41.0 33.0 41.0 26-27 37.63463266710098 39.5 36.0 41.0 32.5 41.0 28-29 37.40524502200117 39.0 36.0 41.0 32.0 41.0 30-31 37.07808768653881 39.0 35.0 41.0 32.0 41.0 32-33 36.67221550710086 38.0 35.0 41.0 31.0 41.0 34-35 36.398254597550334 38.0 35.0 41.0 30.0 41.0 36-37 36.16430916936594 38.0 35.0 41.0 30.0 41.0 38-39 35.9584581653568 38.0 35.0 40.0 30.0 41.0 40-41 35.788081070483784 38.0 35.0 40.0 30.0 41.0 42-43 35.526035139053675 38.0 35.0 40.0 29.0 41.0 44-45 35.182004836913734 38.0 35.0 40.0 26.5 41.0 46-47 34.75902969929878 38.0 34.5 40.0 24.0 41.0 48-49 34.37878434801463 38.0 34.0 40.0 23.0 41.0 50-51 34.15167990046535 38.0 33.0 40.0 23.0 41.0 52-53 34.006761446129815 38.0 33.0 40.0 22.5 41.0 54-55 33.817688676616754 38.0 33.0 40.0 21.5 41.0 56-57 33.541640619538335 37.5 33.0 40.0 20.0 41.0 58-59 33.26342588042671 37.0 33.0 40.0 19.5 41.0 60-61 32.81531176006314 36.0 33.0 40.0 14.5 41.0 62-63 32.44617591006218 36.0 32.0 39.0 10.5 41.0 64-65 32.03011976782003 36.0 31.5 39.0 7.0 40.5 66-67 31.546183711535946 35.0 31.0 39.0 2.0 40.0 68-69 31.067534420203586 35.0 31.0 38.0 2.0 40.0 70-71 30.505457485514082 35.0 30.0 37.0 2.0 39.5 72-73 30.129718245215518 35.0 29.5 37.0 2.0 39.0 74-75 29.723746774293232 34.5 29.0 36.0 2.0 39.0 76-77 27.65245498195023 32.0 26.5 34.5 2.0 36.0 78-79 28.69779268951249 34.0 29.0 35.0 2.0 37.0 80-81 28.69930131216736 34.0 29.0 35.0 2.0 37.0 82-83 28.363783228452483 34.0 29.0 35.0 2.0 36.5 84-85 27.947636913331706 34.0 28.5 35.0 2.0 36.0 86-87 27.434844522706847 34.0 26.5 35.0 2.0 35.5 88-89 26.85267879306135 33.0 25.5 35.0 2.0 35.0 90-91 26.357084092794985 33.0 24.5 35.0 2.0 35.0 92-93 26.079649501060292 33.0 24.0 35.0 2.0 35.0 94-95 25.669190156363825 33.0 22.0 35.0 2.0 35.0 96-97 25.09001279644334 32.5 19.0 35.0 2.0 35.0 98-99 24.36992207644836 32.0 2.0 34.5 2.0 35.0 100-101 21.525039184675066 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15437404870825233 1101 2 0.11727623071972104 1101 3 0.08299277556901785 1101 4 0.11352147063270479 1101 5 0.06087484689930989 1101 6 0.18009067683157554 1101 7 0.23809898553025732 1101 8 0.12256759160868569 1101 9 0.22687234469447048 1101 10-11 0.13201125149490167 1101 12-13 0.2696058531572305 1101 14-15 0.3410744468736624 1101 16-17 0.30651645048693155 1101 18-19 0.3919321541265717 1101 20-21 0.21099585011409516 1101 22-23 0.30160208841355285 1101 24-25 0.37779842250815676 1101 26-27 0.3653040586503451 1101 28-29 0.3597693613954007 1101 30-31 0.17578946855382327 1101 32-33 0.4448802634397424 1101 34-35 0.11379660469891917 1101 36-37 0.018504311858215772 1101 38-39 0.01551432696503241 1101 40-41 0.35264968155946264 1101 42-43 0.16944635293857147 1101 44-45 0.18831171289258464 1101 46-47 -0.0319147038485994 1101 48-49 0.025453475251332236 1101 50-51 -0.03746609751927821 1101 52-53 0.02838019282169313 1101 54-55 -0.1730475805517102 1101 56-57 -0.26100411963452075 1101 58-59 -0.166599762927369 1101 60-61 -0.37553451573722185 1101 62-63 -0.4765317664862536 1101 64-65 -0.3946839256704813 1101 66-67 -0.4381777070572639 1101 68-69 -0.4453776616710421 1101 70-71 -0.7552476969005575 1101 72-73 -0.6768550974745935 1101 74-75 -0.44517573270756117 1101 76-77 -0.32709845937604953 1101 78-79 -0.3197318477196518 1101 80-81 -0.14096704863797527 1101 82-83 -0.3097491675515016 1101 84-85 -0.06647369499780353 1101 86-87 -0.21027349344000612 1101 88-89 0.05446427845772206 1101 90-91 0.02056982459799528 1101 92-93 -0.06844615482543048 1101 94-95 0.002452571870094289 1101 96-97 -0.06651184967276436 1101 98-99 -0.061676531567574244 1101 100-101 0.5310510823512296 1102 1 0.1357549059579206 1102 2 0.14915938455652977 1102 3 0.1458185421100353 1102 4 0.23965398930661763 1102 5 0.07651164086841789 1102 6 0.13575547273414657 1102 7 0.0030577564930140966 1102 8 0.1945880406396867 1102 9 0.13844719152913143 1102 10-11 0.15076314150635284 1102 12-13 0.07796285875999587 1102 14-15 0.2965177989146355 1102 16-17 0.4060620143472917 1102 18-19 0.22856789802317934 1102 20-21 0.26667826628092683 1102 22-23 0.16373602147083943 1102 24-25 0.09804101068686322 1102 26-27 0.16906253244972191 1102 28-29 0.16039130910130694 1102 30-31 0.18027668314721979 1102 32-33 0.24706720015679906 1102 34-35 0.19381998726581173 1102 36-37 0.4857639559765019 1102 38-39 0.33702462172347936 1102 40-41 0.3557481097842654 1102 42-43 0.3837129332500666 1102 44-45 0.286770296937668 1102 46-47 0.250229777556072 1102 48-49 0.38378357423435716 1102 50-51 0.28559013731598526 1102 52-53 0.33967073431146133 1102 54-55 0.1485115271009647 1102 56-57 -0.06780327957650911 1102 58-59 0.14255967903410038 1102 60-61 0.07960524391285162 1102 62-63 -0.08509640547323016 1102 64-65 0.11071466137481423 1102 66-67 0.2959545306448632 1102 68-69 0.1759235710331204 1102 70-71 0.1544288045721629 1102 72-73 0.38817498793606475 1102 74-75 0.2548916528976335 1102 76-77 0.46099702816951904 1102 78-79 0.528472552107953 1102 80-81 0.4956834581827323 1102 82-83 0.5338567004727182 1102 84-85 0.29691389605014606 1102 86-87 0.45345447825327767 1102 88-89 0.5349688847281548 1102 90-91 0.8143282031527086 1102 92-93 0.8686328339618932 1102 94-95 0.7952424311699033 1102 96-97 1.1743190648427593 1102 98-99 1.0822905518738999 1102 100-101 1.3928724938296426 1103 1 -4.044877936628666E-4 1103 2 0.10027027655679532 1103 3 0.12707282650966079 1103 4 0.1803085986163211 1103 5 0.18429304962596404 1103 6 0.15879967365253833 1103 7 0.09797657592376652 1103 8 0.10616038008621587 1103 9 0.1810376451824922 1103 10-11 0.1637172878661417 1103 12-13 0.3000532232039461 1103 14-15 0.2573912530093452 1103 16-17 -0.01317529209174495 1103 18-19 0.17901558843832532 1103 20-21 0.18663629090273304 1103 22-23 0.08466361159582192 1103 24-25 0.23315336759965533 1103 26-27 0.3063065248997887 1103 28-29 0.19425919465032138 1103 30-31 0.09518968048971033 1103 32-33 0.1551333747631034 1103 34-35 0.0030292162147347312 1103 36-37 -0.3258410153356337 1103 38-39 -0.10912927860612598 1103 40-41 -0.03645522195268569 1103 42-43 0.15431443948063617 1103 44-45 0.1963424673558265 1103 46-47 0.22352446912624657 1103 48-49 0.5448793475860967 1103 50-51 0.7337019495818069 1103 52-53 0.46921337171744426 1103 54-55 0.2935214340414589 1103 56-57 0.5268948639285753 1103 58-59 0.6751539827780775 1103 60-61 0.7945565073999106 1103 62-63 0.6175005276009244 1103 64-65 0.6048937469313387 1103 66-67 0.467746043542661 1103 68-69 0.4448148388397897 1103 70-71 0.6263818334666276 1103 72-73 0.6634588147383269 1103 74-75 0.7921823629106868 1103 76-77 0.6744376006122614 1103 78-79 0.6567122625060158 1103 80-81 0.7181330079099411 1103 82-83 0.6080941947210547 1103 84-85 0.6052169451341101 1103 86-87 0.6819385755009293 1103 88-89 0.712603614423621 1103 90-91 0.7970837881317188 1103 92-93 0.553191857729157 1103 94-95 0.4818779214930302 1103 96-97 0.7212291323602038 1103 98-99 1.1541921679729121 1103 100-101 1.3025280046239835 1104 1 -0.003529867087266325 1104 2 0.016700938314560432 1104 3 0.053833760279935916 1104 4 -0.05486355010722832 1104 5 -0.10615144876245353 1104 6 0.07241513399696942 1104 7 0.07206323692631855 1104 8 -0.11901190349728097 1104 9 0.0021526672320959506 1104 10-11 0.06418727478472874 1104 12-13 0.05936024536143236 1104 14-15 0.006917557442193356 1104 16-17 0.13820837097415506 1104 18-19 -0.08152540224285332 1104 20-21 0.06298657613152159 1104 22-23 0.19017466336855904 1104 24-25 0.08588967594344155 1104 26-27 0.17521591250076796 1104 28-29 -0.05375667836555209 1104 30-31 -0.05099850308165088 1104 32-33 -0.019714233495719213 1104 34-35 0.101443263226912 1104 36-37 0.22598060541123033 1104 38-39 0.323735753557969 1104 40-41 0.389896074725705 1104 42-43 0.3533222074136475 1104 44-45 0.22692196055214708 1104 46-47 0.15199306698737303 1104 48-49 0.29263730095152596 1104 50-51 0.24575000018126048 1104 52-53 0.2737793924049541 1104 54-55 0.24121937473005062 1104 56-57 0.28424473109151904 1104 58-59 -0.1052063630028286 1104 60-61 0.049342349802572016 1104 62-63 -0.277629005379076 1104 64-65 -0.1387762642620345 1104 66-67 -0.0713093973819312 1104 68-69 -0.16744258182884764 1104 70-71 0.00384134176725226 1104 72-73 -0.15162122464077754 1104 74-75 -0.2896455962537203 1104 76-77 -0.24124458843441587 1104 78-79 -0.1505340996468867 1104 80-81 -0.23989351904182143 1104 82-83 -0.29536799729253715 1104 84-85 -0.2429341249795769 1104 86-87 -0.02531419249950062 1104 88-89 -0.06341424103348459 1104 90-91 -0.044855295629798064 1104 92-93 -0.18571085430439283 1104 94-95 -0.05989831888134134 1104 96-97 -0.04990679298132861 1104 98-99 -0.04729365150356202 1104 100-101 0.3974879512194427 1105 1 -0.09215481455469643 1105 2 -0.007201659073601263 1105 3 0.12127223880329296 1105 4 0.013732312661019819 1105 5 -0.07708565018956648 1105 6 -0.18035046956612888 1105 7 -0.018109336736024773 1105 8 -0.0326528053008488 1105 9 -0.0766261756504889 1105 10-11 0.10667083681541101 1105 12-13 0.03488896205466574 1105 14-15 -0.03637039219769633 1105 16-17 -0.04388619432299379 1105 18-19 -0.2368689374569044 1105 20-21 -0.05479144624235488 1105 22-23 -0.014933614875062062 1105 24-25 -0.10317643393357656 1105 26-27 -0.10836713120855279 1105 28-29 -0.011168051194481166 1105 30-31 -0.04319477588189358 1105 32-33 8.526441520473327E-5 1105 34-35 0.2626165239819187 1105 36-37 0.39471688857345555 1105 38-39 0.04636378293618293 1105 40-41 -0.04829393641453095 1105 42-43 0.09593429357372685 1105 44-45 0.2789203106403093 1105 46-47 0.42629591252938326 1105 48-49 0.5669901775127713 1105 50-51 0.6697784533777948 1105 52-53 0.4469345263657942 1105 54-55 0.6521891760068428 1105 56-57 0.3401266000725087 1105 58-59 0.5351751179598097 1105 60-61 0.33623244388312656 1105 62-63 0.240061407121118 1105 64-65 0.5279832377403153 1105 66-67 0.3311923759397466 1105 68-69 0.28323961891339167 1105 70-71 0.32681380691787254 1105 72-73 0.24542716758462646 1105 74-75 0.19197912218282198 1105 76-77 -0.04187820349797633 1105 78-79 -0.1316334923814928 1105 80-81 0.07519127797677427 1105 82-83 0.1522428547690744 1105 84-85 0.3041217163086891 1105 86-87 0.24972410902522313 1105 88-89 0.5813360597085264 1105 90-91 0.43895021934504186 1105 92-93 0.4314756902421131 1105 94-95 0.39128863925492396 1105 96-97 0.4516953114345199 1105 98-99 0.664862885549411 1105 100-101 0.6317698726774132 1106 1 -0.04632479785010091 1106 2 -0.19415301393979334 1106 3 -0.21574918568408208 1106 4 -0.11247148343077384 1106 5 0.045868036593624595 1106 6 -0.042431032813773584 1106 7 -0.07167279739052645 1106 8 -0.17626862197629123 1106 9 -0.18162185936182595 1106 10-11 -0.1479290936755291 1106 12-13 -0.16785944876632186 1106 14-15 -0.09434431508748276 1106 16-17 -0.26979345577797176 1106 18-19 -0.5216671930036156 1106 20-21 -0.4027667144688607 1106 22-23 -0.42242938266993946 1106 24-25 -0.31094014795982616 1106 26-27 -0.40457944776628807 1106 28-29 -0.4934309075685732 1106 30-31 -0.353478754042591 1106 32-33 -0.34730254010736417 1106 34-35 -0.3834534111055845 1106 36-37 -0.3037432614315918 1106 38-39 -0.3282239274128145 1106 40-41 -0.434861309756684 1106 42-43 -0.3685482562878164 1106 44-45 -0.4073791164704659 1106 46-47 -0.4329106751361138 1106 48-49 -0.4734769906655245 1106 50-51 -0.3663337477052053 1106 52-53 -0.5234108888387539 1106 54-55 -0.664764869532803 1106 56-57 -0.7295646451487414 1106 58-59 -0.6109138760646502 1106 60-61 -0.5430157371095063 1106 62-63 -0.6104442152652823 1106 64-65 -0.4953017382117899 1106 66-67 -0.6889843116170091 1106 68-69 -0.6371483986208304 1106 70-71 -0.42133467341218633 1106 72-73 -0.5498069770614933 1106 74-75 -0.37884220588875195 1106 76-77 -0.5360030059016445 1106 78-79 -0.49807967330686154 1106 80-81 -0.602258075701041 1106 82-83 -0.39747854841712993 1106 84-85 -0.5710041287309444 1106 86-87 -0.3800863106077266 1106 88-89 -0.1857441111492335 1106 90-91 -0.2104449396849688 1106 92-93 0.09223165737105177 1106 94-95 0.13680754684149932 1106 96-97 0.13053989140918176 1106 98-99 0.13827174459175495 1106 100-101 0.32361376877257086 1107 1 -0.10252749432081387 1107 2 -0.01118364085567336 1107 3 -0.12143781656056873 1107 4 -0.1349113282616372 1107 5 0.018154611207734206 1107 6 -0.20176152680503634 1107 7 -0.11698652660410147 1107 8 -0.03930201589431448 1107 9 -0.04175803927122246 1107 10-11 -0.12012072213767055 1107 12-13 -0.06058928627276572 1107 14-15 -0.17243345596044435 1107 16-17 -0.0616253739615189 1107 18-19 -0.2765162286219933 1107 20-21 -0.15623767451009485 1107 22-23 -0.2327432475208795 1107 24-25 -0.29766667591469087 1107 26-27 -0.3472699504383385 1107 28-29 -0.19964038590728705 1107 30-31 -0.09364911456669489 1107 32-33 -0.020142515150091356 1107 34-35 -0.3090291916985919 1107 36-37 -0.3050074016263693 1107 38-39 -0.38892707148681893 1107 40-41 -0.2142118780772222 1107 42-43 -0.10098186180011481 1107 44-45 -0.13933256572853026 1107 46-47 -0.1464310071763748 1107 48-49 -0.3974820906302625 1107 50-51 -0.3727629585670442 1107 52-53 -0.5647395924168634 1107 54-55 -0.4833363368577679 1107 56-57 -0.44860813048634185 1107 58-59 -0.335141421299717 1107 60-61 -0.42353278672565864 1107 62-63 -0.16873301433209775 1107 64-65 -0.18372915866047634 1107 66-67 -0.08066702870460318 1107 68-69 0.05083955678160734 1107 70-71 0.0254846304968801 1107 72-73 -0.09986801387347555 1107 74-75 0.071541647839922 1107 76-77 0.0049974552653786475 1107 78-79 0.04102186482080228 1107 80-81 -0.26889399789945045 1107 82-83 -0.04018261482518071 1107 84-85 -0.06526395565767373 1107 86-87 -0.2366335259727279 1107 88-89 -0.0034854778312762846 1107 90-91 0.1020716758916258 1107 92-93 0.3564044051126878 1107 94-95 0.15684932314836786 1107 96-97 0.12345419572516292 1107 98-99 0.0917698321049798 1107 100-101 0.4850656310735282 1108 1 -0.10457473260480299 1108 2 -0.08511614102697962 1108 3 -0.10015476109432697 1108 4 -0.016675410931270562 1108 5 -0.09439816421275538 1108 6 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-0.952159691092838 2316 58-59 -0.7528314755941281 2316 60-61 -0.6184358869460809 2316 62-63 -0.5658842276070359 2316 64-65 -0.7194317894107698 2316 66-67 -0.9164770078074582 2316 68-69 -0.7445611626909105 2316 70-71 -0.7448003913067609 2316 72-73 -0.90307272002525 2316 74-75 -0.9505019785305606 2316 76-77 -0.8935469010739006 2316 78-79 -0.7804902679285597 2316 80-81 -0.7717590061533457 2316 82-83 -1.156450422724113 2316 84-85 -1.1782192319484786 2316 86-87 -1.3717913041958028 2316 88-89 -1.6846184084890083 2316 90-91 -2.2175086636154298 2316 92-93 -2.5805677988205566 2316 94-95 -2.5919603028976823 2316 96-97 -2.720909300109721 2316 98-99 -3.065645314986117 2316 100-101 -3.5626719427582856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 21.0 5 123.0 6 363.0 7 672.0 8 1033.0 9 1573.0 10 2462.0 11 3539.0 12 4747.0 13 6395.0 14 7161.0 15 9093.0 16 11279.0 17 14129.0 18 12852.0 19 8862.0 20 8445.0 21 9016.0 22 10003.0 23 11031.0 24 12263.0 25 13597.0 26 15457.0 27 17390.0 28 20818.0 29 24604.0 30 29280.0 31 34133.0 32 41027.0 33 50214.0 34 67827.0 35 106998.0 36 117668.0 37 166312.0 38 135130.0 39 11465.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.13437049361046 21.91958719692147 15.518556409573222 17.427485899894847 2 13.774768412744567 26.893706439660665 37.70016605994166 21.631359087653106 3 22.194382330979046 30.809235729128776 31.27306880595911 15.723313133933067 4 10.687816428282673 19.173388261112287 40.71447315229186 29.42432215831318 5 9.272912776483725 43.87163840067762 34.14907704512595 12.706371777712711 6 23.67727025419633 40.578605927296344 18.53447795475753 17.20964586374979 7 22.126296741719546 38.585278720315145 23.003101339117908 16.285323198847408 8 23.843938102904477 31.397487114903548 22.11738077169747 22.6411940104945 9 24.74840247093951 20.215948846648356 24.601998190463355 30.43365049194878 10-11 22.519359306499638 28.430343477613317 27.875577638341912 21.17471957754513 12-13 27.45465266724283 27.741179522043215 27.833784028863427 16.97038378185053 14-15 22.622164358301504 28.837880829593004 31.45249698578506 17.087457826320428 16-17 16.422959041515742 32.370272803262495 31.878922971706476 19.327845183515286 18-19 15.699721983562176 32.83952656151215 34.75864039424839 16.70211106067729 20-21 18.22550919814021 29.628072336885875 33.230681473931426 18.91573699104249 22-23 16.963798122175135 30.059635681306755 33.72607505828309 19.250491138235024 24-25 18.906111796132294 30.752903009443838 33.60925558742564 16.731729606998226 26-27 18.360888436785707 28.58797234425406 36.82851532737854 16.222623891581694 28-29 15.839777992346452 32.10478696404129 35.93835012001098 16.117084923601283 30-31 16.571748735806636 31.37742618235388 34.34107435412409 17.709750727715395 32-33 16.534869041624408 32.016235170867475 34.63915144281672 16.809744344691403 34-35 18.219658684743152 30.35233441498403 34.29293735291957 17.13506954735325 36-37 17.828296723480214 29.851761888765427 35.677367150896636 16.64257423685772 38-39 17.32014901515332 31.039503809735635 35.52373922044181 16.116607954669238 40-41 16.12856975670255 31.88441407835188 35.503655566639424 16.48336059830614 42-43 15.420666330279506 32.820342912261715 33.25688631127161 18.50210444618717 44-45 16.451387548513157 31.368135650921076 32.91746645936648 19.26301034119929 46-47 17.729190434601115 32.329980323696475 32.38606082835009 17.554768413352328 48-49 17.411387326341043 31.53044806847472 32.864754729521614 18.19340987566262 50-51 15.50073785054233 32.23801493127833 34.43778726905126 17.82345994912808 52-53 16.292466106649186 33.40859560300833 32.44146615021586 17.857472140126625 54-55 15.062467514967178 31.294801482887923 33.3674099005768 20.275321101568096 56-57 17.28294932182903 31.665827417215723 31.60042675074697 19.450796510208278 58-59 18.332888377118884 30.784198600085006 31.826915916446847 19.055997106349263 60-61 16.842639583622514 31.51321848571767 33.422054349136616 18.222087581523198 62-63 16.887455128861095 31.324386292506635 33.1447132857072 18.643445292925076 64-65 16.174481480618404 32.887568604843196 32.50240883167358 18.435541082864823 66-67 16.423289440155546 31.12308816951112 31.633382456271885 20.820239934061448 68-69 15.482185157641549 31.782669662994206 32.10871278822597 20.626432391138273 70-71 17.396066368381806 31.229381464189604 31.085760950620887 20.288791216807702 72-73 16.790747249473906 29.36510188015518 32.73108319917162 21.11306767119929 74-75 15.53237966226711 32.40868982860061 31.400020060932547 20.658910448199734 76-77 16.360201185634264 29.777461590611875 32.2697680503461 21.592569173407757 78-79 16.06121725029139 29.857700298991535 32.909643743982166 21.17143870673491 80-81 16.833452719522835 30.21470263720209 31.83573743684099 21.116107206434087 82-83 16.12554496333814 29.371180950624776 32.81071902232336 21.692555063713726 84-85 15.694437549202478 29.420472080349104 32.28857752790547 22.596512842542957 86-87 16.016993608625462 29.829576874568133 31.984014968337384 22.169414548469025 88-89 16.25167144148773 29.056374398827412 31.49648838465694 23.195465775027916 90-91 16.124345719951886 27.840276423160702 32.2222764069053 23.813101449982117 92-93 16.608577771068287 29.376804090809156 31.894907056078413 22.119711082044148 94-95 16.081573020274714 28.41073847534886 31.91137120526691 23.59631729910952 96-97 14.77746042778419 28.15967286495446 32.523077671270215 24.539789035991134 98-99 16.100771031114803 28.57060355221431 30.804668740311453 24.523956676359436 100-101 15.886877999974672 27.317721855646603 31.89280512671133 24.902595017667398 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11924.0 1 7864.5 2 4786.5 3 5610.5 4 4358.0 5 4394.0 6 8255.0 7 14343.0 8 18259.5 9 17053.0 10 12070.5 11 7941.0 12 6776.0 13 6142.5 14 5277.0 15 4400.5 16 3746.5 17 3341.0 18 3129.0 19 3090.0 20 3080.5 21 3089.5 22 3265.0 23 3529.0 24 3815.5 25 4374.0 26 5646.0 27 7189.5 28 7841.0 29 8765.0 30 11216.0 31 13571.5 32 17061.5 33 21475.0 34 24885.0 35 28000.5 36 32319.0 37 35630.0 38 35061.5 39 32868.0 40 30446.5 41 28098.5 42 25351.5 43 24686.0 44 25144.5 45 25125.0 46 28661.5 47 43942.5 48 77900.5 49 75735.5 50 45003.5 51 31171.0 52 19175.5 53 11308.0 54 7524.5 55 6176.0 56 5444.0 57 4782.0 58 4226.0 59 3639.0 60 3009.0 61 2445.5 62 1976.0 63 1510.5 64 1099.5 65 892.0 66 713.0 67 557.0 68 413.0 69 279.5 70 219.5 71 174.5 72 138.5 73 144.5 74 126.5 75 84.5 76 63.5 77 40.0 78 31.0 79 23.5 80 12.5 81 6.5 82 3.0 83 1.0 84 2.0 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37153252353360156 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.039535234799031E-4 16-17 0.0018237211408794185 18-19 1.013178411599677E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.013178411599677E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007649497007577561 36-37 0.017072056235454557 38-39 0.011499574971656334 40-41 0.007446861325257626 42-43 0.0035461244405988698 44-45 0.0018743800614594024 46-47 0.0019250389820393865 48-49 1.013178411599677E-4 50-51 4.5593028521985463E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.5593028521985463E-4 60-61 4.052713646398708E-4 62-63 0.0 64-65 0.0 66-67 0.001367790855659564 68-69 7.09224888119774E-4 70-71 0.0017224032997194508 72-73 0.0 74-75 0.0 76-77 0.09508679392862969 78-79 0.03475201951786892 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1465055983173133 88-89 0.3228493008562371 90-91 0.2740647603377126 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.039535234799031E-4 100-101 5.572481263798224E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 986993.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.499018912369195 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84170408671922 23.84750957762284 2 9.20727553921539 5.432111905254182 3 2.8353126532379083 2.5091682474103423 4 1.43190545599912 1.6895922450897074 5 0.8903372596219339 1.3132037829987195 6 0.5914600678441022 1.0468495036346603 7 0.4678314421626759 0.9660397992110412 8 0.3565952600180475 0.8415368255466874 9 0.29269049605521735 0.7770674230743221 >10 2.44771482557452 14.958949168264535 >50 0.3730295349831124 7.826624084219922 >100 0.23322932906141322 12.469078026242522 >500 0.013052598680903823 2.552706284913153 >1k 0.013396088119874977 9.966528118538589 >5k 0.0024044260727980726 4.937718015722735 >10k+ 0.0020609366338269195 8.865316992256044 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19461 1.9717465068141315 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18731 1.897784482767355 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13477 1.3654605453128847 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13296 1.3471220160629305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12461 1.2625216186943575 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10067 1.0199667069573948 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8924 0.9041604145115517 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7813 0.7915962929828277 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7581 0.768090553833715 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6826 0.6915955837579395 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6434 0.6518789900232321 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5933 0.6011187516020884 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5220 0.5288791308550314 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4883 0.4947350183841223 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4777 0.48399532722116567 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4714 0.4776123032280878 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4587 0.46474493740077183 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4188 0.42431911877794476 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4114 0.41682159853210715 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3991 0.4043595040694311 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3881 0.3932145415418346 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3798 0.3848051607255573 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3471 0.35167422666624787 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3150 0.31915119965389827 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2975 0.3014205774509039 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2937 0.2975704994868251 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2917 0.2955441426636258 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2626 0.2660606508860752 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2596 0.26302111565127617 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2450 0.24822871084192086 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2364 0.23951537650216365 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2347 0.2377929732024442 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2134 0.2162122730353711 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2105 0.213274055641732 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2025 0.20516862834893457 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1870 0.1894643629691396 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1853 0.18774195966942014 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1822 0.18460110659346116 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 1757 0.17801544691806323 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1742 0.17649567930066373 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1714 0.17365877974818464 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1682 0.17041660883106569 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1596 0.16170327449130845 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1461 0.1480253659347128 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1412 0.1430607917178744 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1401 0.14194629546511475 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1312 0.13292900760187762 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1184 0.11996032393340175 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1173 0.11884582768064211 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1132 0.11469179619308344 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 1118 0.11327334641684389 RNA PCR Primer, Index 26 (100% over 22bp) ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1102 0.1116522609582844 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5197676173995153E-4 0.0 12-13 0.0 0.0 0.0 2.026356823199354E-4 0.0 14-15 0.0 0.0 0.0 2.5329460289991927E-4 0.0 16-17 0.0 0.0 0.0 5.065892057998385E-4 0.0 18-19 0.0 0.0 0.0 8.612016498597255E-4 0.0 20-21 0.0 0.0 0.0 0.0024316281878392246 0.0 22-23 0.0 0.0 0.0 0.006636318595977884 0.0 24-25 0.0 0.0 0.0 0.013627249636015656 0.0 26-27 0.0 0.0 0.0 0.023049808863892653 0.0 28-29 1.013178411599677E-4 0.0 0.0 0.051114850865203704 0.0 30-31 2.026356823199354E-4 0.0 0.0 0.11747803682498256 0.0 32-33 2.026356823199354E-4 0.0 0.0 0.24230161713406276 0.0 34-35 2.026356823199354E-4 0.0 0.0 0.4388582289844001 0.0 36-37 2.026356823199354E-4 0.0 0.0 0.7147467104629921 0.0 38-39 2.026356823199354E-4 0.0 0.0 1.0838982647293345 0.0 40-41 2.026356823199354E-4 0.0 0.0 1.477670054397549 0.0 42-43 2.026356823199354E-4 0.0 0.0 1.8684529677515442 0.0 44-45 2.026356823199354E-4 0.0 0.0 2.308476351909284 0.0 46-47 2.026356823199354E-4 0.0 0.0 2.8487030809742317 0.0 48-49 2.026356823199354E-4 0.0 0.0 3.3725163197712646 0.0 50-51 2.026356823199354E-4 0.0 0.0 3.9350836328119856 0.0 52-53 2.026356823199354E-4 0.0 0.0 4.428400201419868 0.0 54-55 2.026356823199354E-4 0.0 0.0 4.945982392985563 0.0 56-57 2.026356823199354E-4 0.0 0.0 5.596037661867916 0.0 58-59 2.026356823199354E-4 0.0 0.0 6.258858978736424 0.0 60-61 2.026356823199354E-4 0.0 0.0 7.0388037199858555 0.0 62-63 2.026356823199354E-4 0.0 0.0 7.74985232924651 0.0 64-65 2.026356823199354E-4 0.0 0.0 8.394841706070864 0.0 66-67 2.026356823199354E-4 0.0 0.0 9.124380821343212 0.0 68-69 2.026356823199354E-4 0.0 0.0 9.942117117345312 0.0 70-71 2.026356823199354E-4 0.0 0.0 10.831383809206347 0.0 72-73 2.026356823199354E-4 0.0 0.0 11.663456579732582 0.0 74-75 2.026356823199354E-4 0.0 0.0 12.549582418517659 0.0 76-77 2.026356823199354E-4 0.0 0.0 13.345991308955586 0.0 78-79 2.026356823199354E-4 0.0 0.0 14.362006620107742 0.0 80-81 2.026356823199354E-4 0.0 0.0 15.450464187689274 0.0 82-83 2.026356823199354E-4 0.0 0.0 16.40492891033675 0.0 84-85 2.5329460289991927E-4 0.0 0.0 17.490650896206965 0.0 86-87 3.039535234799031E-4 0.0 0.0 18.587973774889996 0.0 88-89 3.039535234799031E-4 0.0 0.0 19.618832149772082 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 150 0.0 41.13005 3 CTTAACG 25 0.0016231773 37.966198 20-21 CATTCCG 125 5.700713E-9 34.16958 9 CCTTTAG 155 3.6379788E-11 33.7944 1 CATGGGG 1275 0.0 31.266283 4 ATGGGGA 590 0.0 29.761639 5 CCCTATA 160 1.9226718E-9 29.661093 2 CTACACT 85 6.4399553E-4 27.916323 4 GCTTTGC 280 0.0 27.211077 1 TTAGCAC 70 0.008427506 27.118713 3 GGATATG 300 0.0 26.984316 1 TACCTGG 365 0.0 26.004246 2 GAGTACG 110 9.31494E-5 25.974209 1 CTTTAGG 165 8.4804924E-8 25.886045 2 CATGGGT 555 0.0 25.652838 4 TGGGTAT 185 9.049472E-9 25.652838 6 GTACATG 9390 0.0 25.609999 1 TGGGGAG 260 0.0 25.554173 6 ACTATAT 225 1.03682396E-10 25.310799 8 GTATAGT 245 1.0913936E-11 25.267427 1 >>END_MODULE