##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938778_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 976952 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.798794618364056 31.0 31.0 34.0 30.0 34.0 2 31.948128464858048 31.0 31.0 34.0 30.0 34.0 3 32.01227388858408 33.0 31.0 34.0 30.0 34.0 4 35.68614527632883 37.0 35.0 37.0 33.0 37.0 5 35.621143106314335 37.0 35.0 37.0 33.0 37.0 6 35.70445426182658 37.0 35.0 37.0 33.0 37.0 7 35.61695763967933 37.0 35.0 37.0 33.0 37.0 8 35.646375666358225 37.0 35.0 37.0 33.0 37.0 9 37.163478860783336 39.0 37.0 39.0 33.0 39.0 10-11 37.09435571041361 39.0 37.0 39.0 33.0 39.0 12-13 37.034305165453375 39.0 37.0 39.0 33.0 39.0 14-15 37.9815405465161 40.0 37.0 41.0 33.0 41.0 16-17 37.97979992875801 40.0 37.0 41.0 33.0 41.0 18-19 38.20974776652282 40.0 37.0 41.0 33.0 41.0 20-21 38.22284257568437 40.0 37.0 41.0 33.0 41.0 22-23 38.09747357086121 40.0 37.0 41.0 33.0 41.0 24-25 37.944767501371615 40.0 37.0 41.0 33.0 41.0 26-27 37.627926448791754 39.5 36.0 41.0 32.5 41.0 28-29 37.38630096463285 39.0 36.0 41.0 32.0 41.0 30-31 37.05642498300838 39.0 35.0 41.0 32.0 41.0 32-33 36.675409334337814 38.5 35.0 41.0 31.0 41.0 34-35 36.439408998599724 38.0 35.0 41.0 30.5 41.0 36-37 36.19065215077097 38.0 35.0 41.0 30.0 41.0 38-39 35.95592413956878 38.0 35.0 40.0 30.0 41.0 40-41 35.763204333478 38.0 35.0 40.0 30.0 41.0 42-43 35.48387126491373 38.0 35.0 40.0 28.5 41.0 44-45 35.14262727339727 38.0 35.0 40.0 26.5 41.0 46-47 34.695789557726485 38.0 34.0 40.0 24.0 41.0 48-49 34.29728533233977 38.0 33.5 40.0 23.0 41.0 50-51 34.07475188136162 38.0 33.0 40.0 23.0 41.0 52-53 33.90830306913748 38.0 33.0 40.0 22.0 41.0 54-55 33.68624507652372 37.5 33.0 40.0 20.5 41.0 56-57 33.39776672753626 37.0 33.0 40.0 20.0 41.0 58-59 33.10650932696795 37.0 33.0 40.0 18.5 41.0 60-61 32.63232123993809 36.0 32.0 40.0 13.5 41.0 62-63 32.27258862257307 36.0 32.0 39.0 8.5 41.0 64-65 31.818072945241937 35.5 31.0 39.0 4.5 40.0 66-67 31.3283185867883 35.0 31.0 38.5 2.0 40.0 68-69 31.0041926317772 35.0 31.0 38.0 2.0 40.0 70-71 30.521730340896994 35.0 30.0 37.0 2.0 39.5 72-73 29.971246284361975 35.0 29.5 37.0 2.0 39.0 74-75 29.474461386025105 34.0 29.0 36.0 2.0 39.0 76-77 27.518395990795863 32.0 26.5 34.5 2.0 36.0 78-79 28.44740478549611 34.0 28.5 35.0 2.0 37.0 80-81 28.447977996871906 34.0 29.0 35.0 2.0 37.0 82-83 28.09302197037316 34.0 28.5 35.0 2.0 36.0 84-85 27.655368431611787 34.0 27.0 35.0 2.0 36.0 86-87 27.097757105773876 33.0 26.0 35.0 2.0 35.5 88-89 26.411755132288995 33.0 24.5 35.0 2.0 35.0 90-91 25.812052178612667 33.0 23.0 35.0 2.0 35.0 92-93 25.510445241936146 32.0 20.0 35.0 2.0 35.0 94-95 25.125694506997274 32.0 19.0 35.0 2.0 35.0 96-97 24.43801896101344 32.0 7.5 34.0 2.0 35.0 98-99 23.625988277827368 32.0 2.0 34.0 2.0 35.0 100-101 20.291875138184885 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.043762653598207635 1101 2 -0.0018342168142915227 1101 3 -0.09214381770131652 1101 4 0.012743485264344656 1101 5 -0.16496246852751995 1101 6 0.14065588423100195 1101 7 0.18501774886397726 1101 8 0.1673417746287953 1101 9 0.1808830712157743 1101 10-11 0.20619958390407334 1101 12-13 0.20656799094428635 1101 14-15 0.2469926457236511 1101 16-17 0.273118947674881 1101 18-19 0.26632423914123393 1101 20-21 0.10817346246738424 1101 22-23 0.09430662268350432 1101 24-25 0.16353547428173698 1101 26-27 0.03855678057968959 1101 28-29 0.1845775109148704 1101 30-31 0.3295464812485278 1101 32-33 0.3606420515785942 1101 34-35 0.5145620781280726 1101 36-37 0.27546439249898214 1101 38-39 0.48254410312890883 1101 40-41 0.25172651181153327 1101 42-43 0.3098083398252527 1101 44-45 0.5438641904629975 1101 46-47 0.5939894539193986 1101 48-49 0.4265670740252858 1101 50-51 0.3705420948761784 1101 52-53 0.07389752310239572 1101 54-55 0.16166883814563704 1101 56-57 0.06264939136499237 1101 58-59 0.10454099884157841 1101 60-61 0.08439849378085285 1101 62-63 -0.18838796906236865 1101 64-65 -0.21496168197196752 1101 66-67 -0.2967670345654021 1101 68-69 -0.42533740903439465 1101 70-71 -0.5044104956624658 1101 72-73 -0.45857629428428837 1101 74-75 -0.5643267295341836 1101 76-77 -0.5914892238196714 1101 78-79 -0.8646558335335683 1101 80-81 -0.7198130813157846 1101 82-83 -0.4526143144078496 1101 84-85 -0.6979540145996346 1101 86-87 -0.7004250831255767 1101 88-89 -0.5460844230324788 1101 90-91 -0.9913906260457317 1101 92-93 -1.0270607774370788 1101 94-95 -0.812332897043472 1101 96-97 -0.5607098229025027 1101 98-99 -0.9698926940517758 1101 100-101 -0.10961454396177217 1102 1 0.25499201041533226 1102 2 0.1711363296828523 1102 3 0.29691376377423495 1102 4 0.18544892573022054 1102 5 -0.14004250158780707 1102 6 -0.07592683219242957 1102 7 0.1224014865025751 1102 8 -0.0048735538464868 1102 9 0.2251078811302918 1102 10-11 0.2573856549213005 1102 12-13 0.21423267414258618 1102 14-15 0.3063056646575717 1102 16-17 0.3328569159824539 1102 18-19 0.32010177730870026 1102 20-21 0.4100429055442092 1102 22-23 0.30754766644382414 1102 24-25 0.3797400247487914 1102 26-27 0.3014322453506679 1102 28-29 0.34237630657604257 1102 30-31 0.3041057703188059 1102 32-33 0.38160826281240645 1102 34-35 0.46880789602473527 1102 36-37 0.5031258676915158 1102 38-39 0.59651017846803 1102 40-41 0.7052967548847988 1102 42-43 0.5385747947783983 1102 44-45 0.809320540420778 1102 46-47 0.8901552187057433 1102 48-49 0.7161341491928752 1102 50-51 0.7584674062643799 1102 52-53 0.640875225234609 1102 54-55 0.6637891294503717 1102 56-57 0.5806999932002341 1102 58-59 0.6244540262410609 1102 60-61 0.7455528857412403 1102 62-63 0.8251335524234733 1102 64-65 0.8364092949326256 1102 66-67 0.7601082341607786 1102 68-69 0.7224759117639543 1102 70-71 0.9238248561154165 1102 72-73 0.7568802784516713 1102 74-75 0.7525345710002611 1102 76-77 0.4840905006928331 1102 78-79 0.3189713070862439 1102 80-81 0.5602302267619308 1102 82-83 0.5616317931004424 1102 84-85 0.8642267164246782 1102 86-87 0.6422829415671565 1102 88-89 0.5412618878722881 1102 90-91 0.7657850590528597 1102 92-93 0.8608728881327785 1102 94-95 1.0809073447154098 1102 96-97 1.16105360003964 1102 98-99 1.105518832936685 1102 100-101 1.5305719659284627 1103 1 0.35200346915668845 1103 2 0.22690625007301435 1103 3 0.17031370529437595 1103 4 0.1422643114489972 1103 5 0.0559245952184213 1103 6 0.19656060291424637 1103 7 0.09782560337401236 1103 8 0.10629775350928128 1103 9 0.171362687788168 1103 10-11 0.23785062493657705 1103 12-13 0.22837045488726204 1103 14-15 0.2070598341057064 1103 16-17 0.09728019979181113 1103 18-19 0.16486314088237464 1103 20-21 0.42746383360847773 1103 22-23 0.44676447774698147 1103 24-25 0.3421054797055163 1103 26-27 0.3416001818393397 1103 28-29 0.33929287347755377 1103 30-31 0.3359028121208709 1103 32-33 0.4645067671625398 1103 34-35 0.4090519666639878 1103 36-37 0.4777180258740046 1103 38-39 0.39821050783098855 1103 40-41 0.3504851317378126 1103 42-43 0.38970163537665314 1103 44-45 0.5635021258868775 1103 46-47 0.5125066605651867 1103 48-49 0.38509844889660627 1103 50-51 0.5688911645329355 1103 52-53 0.5096938769981065 1103 54-55 0.642858858401766 1103 56-57 0.5562515520193827 1103 58-59 0.4319675841885342 1103 60-61 0.629745670651225 1103 62-63 0.5718170274509262 1103 64-65 0.46959661252788365 1103 66-67 0.5911113843491798 1103 68-69 0.46243696197387507 1103 70-71 0.5895015949256255 1103 72-73 0.5135753942804051 1103 74-75 0.74572994336798 1103 76-77 0.8249248868474517 1103 78-79 0.31836664745555154 1103 80-81 0.4349338276865744 1103 82-83 0.5369759139441008 1103 84-85 0.7748507406110612 1103 86-87 0.7783076355133858 1103 88-89 0.9478160451830995 1103 90-91 0.9354985629340469 1103 92-93 0.9137215742669831 1103 94-95 1.010237750378021 1103 96-97 1.0616571562768478 1103 98-99 1.0386901513263922 1103 100-101 1.304114245208389 1104 1 -0.008419988974956993 1104 2 0.09501846977073569 1104 3 -0.06323915013934212 1104 4 0.06032549225837869 1104 5 0.09226757654806761 1104 6 0.0025370920972918043 1104 7 0.11954511998634842 1104 8 -0.0774027305369458 1104 9 0.003997940842268122 1104 10-11 0.021225667468989684 1104 12-13 -0.09892108143815648 1104 14-15 -0.035395740109223084 1104 16-17 -0.0624748135441493 1104 18-19 0.21191441026892477 1104 20-21 0.16295781321487368 1104 22-23 0.1344624592412771 1104 24-25 -0.03757390826687157 1104 26-27 0.026065700405418113 1104 28-29 -0.033099478372470514 1104 30-31 -0.1369615122449872 1104 32-33 -0.03430013022185818 1104 34-35 -0.02400037838085467 1104 36-37 0.019316915092176146 1104 38-39 0.003414279220670835 1104 40-41 0.044006485750124114 1104 42-43 0.018506776584246154 1104 44-45 0.0039776107563156415 1104 46-47 0.1762712450647328 1104 48-49 0.04462900088493171 1104 50-51 -0.17256448651473733 1104 52-53 -0.06324618249238512 1104 54-55 -0.09056832062947962 1104 56-57 -0.2593142113138427 1104 58-59 0.014806443990742935 1104 60-61 0.15462356148288592 1104 62-63 -0.048396567552750014 1104 64-65 -0.08882315763247917 1104 66-67 -0.2370895222770919 1104 68-69 -0.31171101787399635 1104 70-71 -0.24579316837466791 1104 72-73 -0.07239659469958681 1104 74-75 0.03476865609038171 1104 76-77 0.1460374248522136 1104 78-79 -0.22590766736109202 1104 80-81 -0.04630197197755592 1104 82-83 -0.14525706486104184 1104 84-85 -0.1811559328607899 1104 86-87 -0.004813858993070852 1104 88-89 0.09075456801888393 1104 90-91 0.018035579848223904 1104 92-93 0.24169966596136305 1104 94-95 0.1413637889919599 1104 96-97 0.013933051652614381 1104 98-99 0.08864130765905642 1104 100-101 0.7931793005828389 1105 1 0.10127784243059423 1105 2 0.08043521649478436 1105 3 0.14674389904364205 1105 4 -7.294719399624228E-4 1105 5 -0.01853824906773127 1105 6 0.037352447744218864 1105 7 0.036266225674900454 1105 8 0.0113456843268267 1105 9 0.04099726195564557 1105 10-11 0.2279989492240162 1105 12-13 0.06378327838029207 1105 14-15 0.1965411789410183 1105 16-17 0.09826760662262046 1105 18-19 0.14964257381654988 1105 20-21 0.1485901811628807 1105 22-23 0.11710601814429822 1105 24-25 0.09391981309926933 1105 26-27 0.03715738425773907 1105 28-29 0.06799165041530841 1105 30-31 -0.03829345364724901 1105 32-33 0.06915856230227746 1105 34-35 -0.08833876280147024 1105 36-37 -0.07116607644949369 1105 38-39 -0.009466420331435188 1105 40-41 0.06716228382618539 1105 42-43 0.10226494777468531 1105 44-45 0.19561257469847249 1105 46-47 -0.08856071982103941 1105 48-49 -0.11356545957098518 1105 50-51 -0.26520796002321134 1105 52-53 -0.26037735278190866 1105 54-55 -0.028101144157382407 1105 56-57 0.062201584541959676 1105 58-59 0.12874624937657586 1105 60-61 -0.06659313998205363 1105 62-63 -0.09879215777849737 1105 64-65 -0.18592636120880002 1105 66-67 -0.160111677891571 1105 68-69 -0.277376006537871 1105 70-71 -0.16251213919962382 1105 72-73 -0.05265017379842263 1105 74-75 -0.052700975474859746 1105 76-77 -0.1264160030982744 1105 78-79 -0.4877252048386218 1105 80-81 -0.42690759008461754 1105 82-83 -0.3586889861955882 1105 84-85 -0.37248134158042845 1105 86-87 -0.3452733670351016 1105 88-89 -0.1085082635788055 1105 90-91 -0.1663227015011799 1105 92-93 -0.20191965370030474 1105 94-95 0.00968326706050604 1105 96-97 0.25338575801920626 1105 98-99 0.17194688776554656 1105 100-101 0.9310616012826465 1106 1 0.1880223277433366 1106 2 0.14735114601216992 1106 3 0.1170512218998283 1106 4 0.17657443621782676 1106 5 0.06332737191088 1106 6 -0.015457838908055521 1106 7 2.923273457753339E-4 1106 8 0.014270976400062807 1106 9 0.1905651050213777 1106 10-11 0.20751278533875706 1106 12-13 0.13166028844199928 1106 14-15 0.13156818241650825 1106 16-17 0.25182162877256786 1106 18-19 0.07844965855579034 1106 20-21 0.11894360751382038 1106 22-23 0.21174585359028697 1106 24-25 0.15574409292215563 1106 26-27 0.15403278323028502 1106 28-29 0.33045225979204673 1106 30-31 0.20468716261968467 1106 32-33 0.34121224500891145 1106 34-35 0.2822826717771676 1106 36-37 0.27944238835478785 1106 38-39 0.37560364684794223 1106 40-41 0.5763870358299172 1106 42-43 0.5334775203496918 1106 44-45 0.490685580342344 1106 46-47 0.4815905604375459 1106 48-49 0.39792697641608044 1106 50-51 0.258875796347688 1106 52-53 0.42485888963127394 1106 54-55 0.5627359129197558 1106 56-57 0.573153951019151 1106 58-59 0.46588438760600326 1106 60-61 0.6768348321734621 1106 62-63 0.4317060639438566 1106 64-65 0.5135001315818251 1106 66-67 0.35845789785495086 1106 68-69 0.049858493061890385 1106 70-71 0.3388152361436205 1106 72-73 0.18657427422622064 1106 74-75 0.31573332957828626 1106 76-77 0.3183895009497171 1106 78-79 0.25357542034502956 1106 80-81 -0.06959587979694959 1106 82-83 0.1922128184269205 1106 84-85 0.272878924299949 1106 86-87 0.4478551857204671 1106 88-89 0.29143043397213475 1106 90-91 0.2837416407387394 1106 92-93 0.4221245853893798 1106 94-95 0.5562954201443766 1106 96-97 0.543189286203134 1106 98-99 0.3191178557362804 1106 100-101 0.9479917164907228 1107 1 0.014255619117790275 1107 2 0.1382101042331101 1107 3 0.09118357219654172 1107 4 0.15527131327830546 1107 5 0.02327462360421606 1107 6 0.15743972148275986 1107 7 0.1755995262908101 1107 8 0.1125836299995413 1107 9 0.10640757939648893 1107 10-11 0.029084145405896322 1107 12-13 0.18537964347407154 1107 14-15 0.12062141025322859 1107 16-17 0.07936613515630597 1107 18-19 0.19512082081676851 1107 20-21 0.2687173292665008 1107 22-23 0.18058089524945586 1107 24-25 0.27110104311096705 1107 26-27 0.23498944570106772 1107 28-29 0.24818288378054376 1107 30-31 0.09756464268921405 1107 32-33 0.0848210084207679 1107 34-35 0.2945031956637578 1107 36-37 0.3265142579226179 1107 38-39 0.22106159805576198 1107 40-41 -0.008846435368305094 1107 42-43 0.16371338919822165 1107 44-45 0.17619478132538546 1107 46-47 0.21332196366238776 1107 48-49 0.2938554664958062 1107 50-51 0.03505214352744446 1107 52-53 -0.08269820441655895 1107 54-55 -0.07725090000358392 1107 56-57 0.016130334163669602 1107 58-59 0.2114262565744056 1107 60-61 0.15175990560150154 1107 62-63 0.2867111589507445 1107 64-65 0.14031035756037014 1107 66-67 0.20080040220069506 1107 68-69 0.09248122278675908 1107 70-71 0.06964152637918986 1107 72-73 0.02683681693436668 1107 74-75 0.18703495398636605 1107 76-77 -0.05509098817094937 1107 78-79 0.007368203417620123 1107 80-81 0.09713971931591914 1107 82-83 0.1056111880247137 1107 84-85 0.0276067909905926 1107 86-87 -0.14436893464158018 1107 88-89 0.040288553015475514 1107 90-91 0.25291535050317293 1107 92-93 0.3213168769436585 1107 94-95 0.45866759541261004 1107 96-97 0.4644489517309154 1107 98-99 0.6121946404872247 1107 100-101 1.2576377633647873 1108 1 -0.021733588513161095 1108 2 0.06385719633685483 1108 3 -0.03413627710461142 1108 4 -0.12033270364317161 1108 5 0.0538772904080389 1108 6 -0.1329716572231021 1108 7 -0.07830295663984543 1108 8 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127.0 6 358.0 7 660.0 8 1003.0 9 1557.0 10 2413.0 11 3417.0 12 4779.0 13 6245.0 14 7197.0 15 9233.0 16 11435.0 17 14420.0 18 12914.0 19 9028.0 20 8821.0 21 9426.0 22 10334.0 23 11452.0 24 12500.0 25 14065.0 26 15751.0 27 18087.0 28 21511.0 29 25155.0 30 29978.0 31 35177.0 32 42067.0 33 51448.0 34 69399.0 35 108789.0 36 118160.0 37 160226.0 38 120974.0 39 8805.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.03098071399261 22.002769798933112 15.54965121050472 17.41659827656955 2 13.838346203293508 26.919951031371042 37.68076630172209 21.560936463613363 3 22.196894013216617 30.789230177122317 31.363362785479737 15.65051302418133 4 10.636039436942655 19.240351624235377 40.64068654345352 29.482922395368448 5 9.203393040887473 43.935988860505475 34.15012721343702 12.710490885170037 6 23.642300723710473 40.58449601293875 18.546641962923914 17.22656130042686 7 22.160556506358553 38.613667815819 22.91791203662002 16.307863641202434 8 23.902095497015207 31.370835005199847 22.091566422915353 22.635503074869597 9 24.665694936905805 20.221873746100115 24.624955985555072 30.487475331439008 10-11 22.533041541447275 28.434303834784103 27.909201270891508 21.12345335287711 12-13 27.425451813395128 27.743123510674017 27.866466315642942 16.964958360287916 14-15 22.580485018711258 28.969335238578765 31.389362015738747 17.060817726971234 16-17 16.416810442115263 32.42570976279226 31.866610504248776 19.290869290843702 18-19 15.653327901473151 32.79987143687714 34.89117172593945 16.65562893571025 20-21 18.24946363792694 29.594493895298847 33.28126663336582 18.874775833408396 22-23 16.90236572523522 30.177685290577223 33.74152466037226 19.178424323815296 24-25 18.892020518972576 30.755735071260084 33.6695499613849 16.682694448382442 26-27 18.258011486875873 28.704855257379926 36.824485818488526 16.21264743725568 28-29 15.790182117442821 32.17430334346007 35.969576806229995 16.065937732867123 30-31 16.49098420393223 31.431175738419082 34.41039068449627 17.667449373152415 32-33 16.501066582595662 32.03233116877799 34.700783661838045 16.7658185867883 34-35 18.16893875221685 30.45114994740977 34.323479809702704 17.056431490670672 36-37 17.769310188969758 29.85908528542025 35.79414954906166 16.57745497654834 38-39 17.23498110225258 31.06422162983346 35.622305074751544 16.078492193162415 40-41 16.10232776680464 31.986563351402758 35.47071520006797 16.440393681724633 42-43 15.435428518782965 32.85082904034006 33.26449189129706 18.44925054957991 44-45 16.439839720672992 31.344291929887774 33.01797128627282 19.197897063166415 46-47 17.737424234747277 32.35723275075401 32.41440055437928 17.490942460119445 48-49 17.4602776533811 31.544648454993286 32.91459279636619 18.080481095259422 50-51 15.550508110940967 32.253171087218206 34.45051548080151 17.745805321039317 52-53 16.285191084106486 33.43618724359027 32.44499217975908 17.83362949254416 54-55 15.058109303220629 31.375543527215257 33.39186572114086 20.174481448423258 56-57 17.312473706244887 31.633984787239378 31.652511968456704 19.401029538059028 58-59 18.308632282749663 30.768648107526598 31.885185408859623 19.03753420086412 60-61 16.833413175123756 31.549125220588536 33.44040322151388 18.17705838277383 62-63 17.01296693983086 31.28970280854827 33.117234729452804 18.580095522168065 64-65 16.148629317752718 32.90087779143151 32.63468962541731 18.315803265398458 66-67 16.420377132129317 31.195394815636007 31.667705668651518 20.716522383583154 68-69 15.526324005156864 31.75851795971239 32.21370990648963 20.501448128641115 70-71 17.43980256962471 31.240941213079044 31.158849155332096 20.16040706196415 72-73 16.79099894365332 29.40031854175026 32.746337588745405 21.062344925851015 74-75 15.518730004703421 32.452222404763404 31.460409632646964 20.568637957886214 76-77 16.376077164622092 29.782897688051897 32.29558196078271 21.545443186543302 78-79 16.052186248553486 29.87087198820034 32.974946213744886 21.101995549501293 80-81 16.891287069019786 30.22935284450652 31.82821542063034 21.051144665843353 82-83 16.10964167927398 29.425014279192503 32.80498615071867 21.660357890814847 84-85 15.734140469542005 29.419101450224783 32.318629779149845 22.52812830108337 86-87 16.076773764216675 29.843787541220102 31.99417424300251 22.085264451560715 88-89 16.284965587078837 29.101474340639395 31.486474897798526 23.12708517448324 90-91 16.12151753081473 27.979699134182457 32.197265650082315 23.701517684920496 92-93 16.60561760546765 29.41773167722081 31.822264576412323 22.15438614089922 94-95 16.139362301038947 28.491273862531347 31.81083985874405 23.558523977685656 96-97 14.787435603218585 28.20709534050326 32.476500868518485 24.528968187759673 98-99 16.09777143605827 28.53205684619101 30.71650398381906 24.653667733931655 100-101 15.964157416254965 27.19352344083072 31.868996871348603 24.973322271565713 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11613.0 1 7653.5 2 4690.0 3 5497.0 4 4189.5 5 4314.0 6 8313.0 7 14360.0 8 18055.0 9 16745.5 10 11826.5 11 7903.5 12 6904.0 13 6160.0 14 5300.0 15 4496.0 16 3869.0 17 3466.5 18 3142.5 19 3005.5 20 3035.5 21 3082.5 22 3181.5 23 3481.0 24 3811.0 25 4394.5 26 5740.5 27 7218.5 28 7778.0 29 8878.5 30 11389.0 31 13565.0 32 16827.0 33 21336.5 34 25056.0 35 28122.0 36 32126.0 37 35213.0 38 34550.5 39 32325.0 40 30227.5 41 28208.0 42 25396.0 43 24539.0 44 24868.5 45 24916.0 46 28605.5 47 43465.5 48 76388.0 49 74382.0 50 44308.5 51 30314.5 52 18450.5 53 11023.5 54 7544.5 55 6139.5 56 5342.0 57 4669.0 58 4096.0 59 3589.0 60 3002.5 61 2441.5 62 1951.5 63 1449.0 64 1055.0 65 845.5 66 663.0 67 517.5 68 395.5 69 284.0 70 202.5 71 149.5 72 126.5 73 155.0 74 137.0 75 80.5 76 57.0 77 45.5 78 40.0 79 28.5 80 17.0 81 8.5 82 5.0 83 4.5 84 4.0 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22027694298184558 2 0.0 3 0.0 4 0.0 5 0.025282716039273165 6 0.004299085318418919 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0024054405948296335 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.558979356201737E-4 26-27 0.0011259509167287646 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006090370867760135 36-37 0.015916851595574807 38-39 0.011873664212776063 40-41 0.007318680958736969 42-43 0.007113962610240831 44-45 0.003326673163062259 46-47 3.5825710986824326E-4 48-49 0.001484208026597008 50-51 0.0 52-53 0.0 54-55 0.0 56-57 9.212325682326256E-4 58-59 3.070775227442085E-4 60-61 0.00204718348496139 62-63 6.14155045488417E-4 64-65 0.0011771305038527993 66-67 0.005425036235147684 68-69 1.5353876137210424E-4 70-71 0.0 72-73 0.0 74-75 3.5825710986824326E-4 76-77 0.055888109139445946 78-79 0.1362400609241805 80-81 7.676938068605213E-4 82-83 6.14155045488417E-4 84-85 0.0 86-87 0.09637116255455744 88-89 0.33394680598432674 90-91 0.36793005183468586 92-93 5.629754583643823E-4 94-95 2.0471834849613904E-4 96-97 1.0235917424806952E-4 98-99 0.0 100-101 0.0013818488523489381 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 976952.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.38035044226932 #Duplication Level Percentage of deduplicated Percentage of total 1 80.76742162769305 23.729751517401443 2 9.189675677929882 5.3999178373675765 3 2.846775844731127 2.5091781584636337 4 1.4614835575465952 1.7175559634533382 5 0.8816618755243011 1.2951767437246198 6 0.634199333809566 1.1179799206547274 7 0.48194571036005984 0.991181370516889 8 0.3504320970987715 0.8236654255185006 9 0.30067919516476016 0.7950654112176129 >10 2.447269552061454 14.92928837227896 >50 0.36876912317555116 7.663636052668924 >100 0.23968248404875936 12.81287368308984 >500 0.011862015222209338 2.3428083272056104 >1k 0.013606429225475418 9.99834659836759 >5k 0.0027910624052257266 5.9317454973338055 >10k+ 0.0017444140032660792 7.941829120736939 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19359 1.9815712542683777 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18513 1.8949753928545108 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13550 1.3869668110613418 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13509 1.382770084917171 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12548 1.2844029184647763 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9653 0.9880731090166149 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8899 0.9108942916335705 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7702 0.7883703600586314 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7164 0.73330112431317 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6849 0.701057984425028 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6411 0.6562246661043736 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5985 0.612619657874696 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5206 0.5328818611354498 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4864 0.4978750235426101 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4779 0.48917449373152416 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4676 0.478631498783973 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4532 0.463891777692251 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4299 0.4400420900924508 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3959 0.40523997084810714 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3943 0.4036022240601381 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3895 0.39868898369623074 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3760 0.38487049517274136 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3535 0.3618396809669257 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3108 0.318132313563 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2963 0.3032902332970299 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2927 0.29960530302409943 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2872 0.2939755484404556 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2563 0.2623465635978021 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2561 0.262141845249306 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2477 0.2535436746124682 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2337 0.23921339021773844 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2336 0.23911103104349035 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2169 0.22201704894406274 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2143 0.21935571041361296 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1992 0.20389947510215445 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1859 0.1902857049271612 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1819 0.18619133795723844 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1740 0.17810496319164093 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1679 0.1718610535625087 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1655 0.16940443338055503 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 1647 0.1685855599865705 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1584 0.1621369320089421 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1558 0.15947559347849227 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1536 0.15722369164503475 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1482 0.15169629623563902 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1475 0.1509797820159025 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1247 0.12764189028734269 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1208 0.12364988249166796 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1167 0.1194531563474971 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1116 0.11423283846084557 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1043 0.1067606187407365 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 1037 0.10614646369524808 RNA PCR Primer, Index 26 (100% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.117958712403475E-5 0.0 12-13 0.0 0.0 0.0 1.5353876137210424E-4 0.0 14-15 0.0 0.0 0.0 3.0707752274420854E-4 0.0 16-17 0.0 0.0 0.0 0.0011771305038527995 0.0 18-19 0.0 0.0 0.0 0.0013306692652249037 0.0 20-21 0.0 0.0 0.0 0.0024054405948296335 0.0 22-23 0.0 0.0 0.0 0.006295089216256274 0.0 24-25 0.0 0.0 0.0 0.014688541504597974 0.0 26-27 0.0 0.0 0.0 0.024003226361172296 0.0 28-29 0.0 0.0 0.0 0.04954184033606564 0.0 30-31 0.0 0.0 0.0 0.1114691407561477 0.0 32-33 0.0 0.0 0.0 0.2229382815122954 0.0 34-35 0.0 0.0 0.0 0.4066218197004561 0.0 36-37 0.0 0.0 0.0 0.6623150369721338 0.0 38-39 0.0 0.0 0.0 1.0076237112979962 0.0 40-41 0.0 0.0 0.0 1.3891163537205513 0.0 42-43 0.0 0.0 0.0 1.7460939739106935 0.0 44-45 0.0 0.0 0.0 2.161313964247988 0.0 46-47 1.023591742480695E-4 0.0 0.0 2.6615432487983033 0.0 48-49 1.023591742480695E-4 0.0 0.0 3.164689769814689 0.0 50-51 1.023591742480695E-4 0.0 0.0 3.67843046536575 0.0 52-53 1.023591742480695E-4 0.0 0.0 4.144778863239955 0.0 54-55 1.023591742480695E-4 0.0 0.0 4.634311614081347 0.0 56-57 1.023591742480695E-4 0.0 0.0 5.265663000843439 0.0 58-59 1.023591742480695E-4 0.0 0.0 5.917588581629394 0.0 60-61 1.023591742480695E-4 0.0 0.0 6.684207617160311 0.0 62-63 1.023591742480695E-4 0.0 0.0 7.376206814664385 0.0 64-65 1.023591742480695E-4 0.0 0.0 8.014160368165477 0.0 66-67 1.023591742480695E-4 0.0 0.0 8.724840114969824 0.0 68-69 1.023591742480695E-4 0.0 0.0 9.530202097953635 0.0 70-71 1.023591742480695E-4 0.0 0.0 10.391861626773885 0.0 72-73 1.023591742480695E-4 0.0 0.0 11.223222840016705 0.0 74-75 1.023591742480695E-4 0.0 0.0 12.107452566758653 0.0 76-77 1.023591742480695E-4 0.0 0.0 12.907491872681565 0.0 78-79 1.023591742480695E-4 0.0 0.0 13.938760553230864 0.0 80-81 1.023591742480695E-4 0.0 0.0 15.01624440095317 0.0 82-83 1.023591742480695E-4 0.0 0.0 15.988247119612836 0.0 84-85 1.023591742480695E-4 0.0 0.0 17.06317198797894 0.0 86-87 1.023591742480695E-4 0.0 0.0 18.1573915606908 0.0 88-89 1.023591742480695E-4 0.0 0.0 19.1861012618839 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 190 3.8744474E-10 27.547897 1 CGCTAGG 35 0.008428833 27.11915 36-37 AAGTTCG 35 0.008435149 27.114983 14-15 CATATAG 200 7.221388E-10 26.111538 3 ATGGGAG 1295 0.0 23.829412 5 CTTTAGG 180 1.960143E-7 23.73776 2 CATTCCG 140 1.7940396E-5 23.724398 9 GGGATAG 120 1.7231144E-4 23.724398 7 GTACATG 9695 0.0 23.656399 1 AGGCTTA 410 0.0 23.146938 6 TACATGG 9535 0.0 22.704601 2 ATGGGAT 2040 0.0 22.574144 5 GTAAGCG 95 3.3229298E-7 22.476318 94-95 GGGATAT 150 3.0579693E-5 22.142773 7 ACATGGG 9270 0.0 22.0733 3 GAGTACT 10120 0.0 21.850069 12-13 GTATAGT 220 5.5342753E-8 21.628515 1 CATGGGG 1405 0.0 21.62586 4 TGGGATA 485 0.0 21.524265 6 GGACTAA 375 0.0 21.51012 8 >>END_MODULE