FastQCFastQC Report
Thu 26 May 2016
SRR938777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687663
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67620.9833304976420136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50410.7330625611673159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37090.5393630310195546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22290.3241413308553754No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17240.25070419667773314No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16270.2365984501129187No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11330.16476093667973993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.16010749451402795No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10640.15472695200992348No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.142657086392608No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCGG600.004003623531.5793155
GTATCAA82450.026.3838061
GGTATCA60750.025.5103761
CACACCG1151.3052669E-424.7142495
GAGTACT51250.022.85162212-13
TATCAAC96450.022.493512
ATCAACG96900.022.4379373
TCAACGC97900.022.2571284
CAACGCA98500.022.1696435
GTACATG40450.022.1441331
AACGCAG99350.022.051246
AGTACTT52200.021.34794812-13
TACATGG41450.021.1417622
GTACTTT56350.020.61622214-15
ACATGGG41350.020.3909973
ACGCAGA108750.020.0581827
ATATAGG2601.535409E-820.040722
CGCAGAG109100.019.9504648
CCGGGTC2451.8260653E-719.313189
GTCGGGA2702.3685061E-819.2984712
Produced by FastQC (version 0.11.5)