FastQCFastQC Report
Thu 26 May 2016
SRR938777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687663
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61350.8921521152075944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.7451324267846314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37940.5517237367722271No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23240.3379562372848328No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT18550.26975422554361655No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16870.24532365417362867No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.22452858449560323No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10830.15748993329581495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.15458153194224497No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.1509460302502825No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT9710.141202885715823RNA PCR Primer, Index 17 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.12186201671458258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58400.026.8353941
GTATCAA79350.025.5733491
GAGTACT49200.022.95240612-13
CTACACG1252.2714619E-422.7749584
GTACATG39000.022.3517441
GTGTAGC3007.2759576E-1222.2296051
TACACGG1503.0567506E-522.142325
TACATGG39700.021.3933542
AGTACTT49700.021.14626512-13
ATCAACG95150.021.1433323
TATCAAC95500.021.1155262
TCAACGC95450.021.0768784
ACATGGG39050.021.0204223
CAACGCA95900.020.9779765
GCGGTAT1150.003661129220.710811
GTACTTT54000.020.69252614-15
AACGCAG97700.020.5914846
AGAGTAC78600.019.95102710-11
CATGGGG10550.019.3389264
CGCAGAG104950.019.0333848
Produced by FastQC (version 0.11.5)