##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938777_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 687663 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.939352560774683 31.0 31.0 34.0 30.0 34.0 2 32.154671692384206 33.0 31.0 34.0 30.0 34.0 3 32.2223676422899 34.0 31.0 34.0 30.0 34.0 4 35.888592231950824 37.0 35.0 37.0 35.0 37.0 5 35.79946427247067 37.0 35.0 37.0 35.0 37.0 6 35.8345163255839 37.0 35.0 37.0 35.0 37.0 7 35.76662842118887 37.0 35.0 37.0 33.0 37.0 8 35.74386727219583 37.0 35.0 37.0 33.0 37.0 9 37.365654397575554 39.0 37.0 39.0 34.0 39.0 10-11 37.307305322519895 39.0 37.0 39.0 34.0 39.0 12-13 37.239280723261245 39.0 37.0 39.0 33.0 39.0 14-15 38.40274233163628 40.0 38.0 41.0 33.0 41.0 16-17 38.35434580601254 40.0 38.0 41.0 33.0 41.0 18-19 38.41659650148401 40.0 38.0 41.0 33.5 41.0 20-21 38.47293805250537 40.0 38.0 41.0 34.0 41.0 22-23 38.40699514151554 40.0 38.0 41.0 34.0 41.0 24-25 38.31466212374375 40.0 38.0 41.0 33.0 41.0 26-27 38.11947131080195 40.0 37.0 41.0 33.0 41.0 28-29 37.93573741789219 40.0 37.0 41.0 33.0 41.0 30-31 37.620835351036774 40.0 36.5 41.0 33.0 41.0 32-33 37.29585494639089 39.5 35.5 41.0 32.0 41.0 34-35 37.136819924875994 39.5 35.0 41.0 31.0 41.0 36-37 36.97908714006715 40.0 35.0 41.0 31.0 41.0 38-39 36.8134471390783 39.0 35.0 41.0 31.0 41.0 40-41 36.58922684512618 39.0 35.0 41.0 30.5 41.0 42-43 36.323675986638804 39.0 35.0 41.0 30.0 41.0 44-45 35.95829134328879 39.0 35.0 41.0 29.5 41.0 46-47 35.46655992833699 39.0 35.0 41.0 26.5 41.0 48-49 35.02263390643382 39.0 35.0 41.0 24.5 41.0 50-51 34.711482223123824 39.0 35.0 41.0 23.5 41.0 52-53 34.48798466690806 38.5 34.0 40.0 23.0 41.0 54-55 34.24086071229657 38.0 34.0 40.0 22.0 41.0 56-57 33.95453732424167 38.0 34.0 40.0 20.5 41.0 58-59 33.61868167983445 38.0 33.5 40.0 18.5 41.0 60-61 33.25287168278648 37.0 33.0 40.0 15.5 41.0 62-63 32.86025422336232 37.0 33.0 40.0 10.5 41.0 64-65 32.4721564196416 36.0 32.0 39.5 7.5 41.0 66-67 32.04721935017589 36.0 32.0 39.0 3.5 40.5 68-69 31.580201348625707 35.0 31.0 39.0 2.0 40.0 70-71 31.10439561238572 35.0 31.0 38.0 2.0 40.0 72-73 30.771216424324123 35.0 31.0 37.0 2.0 39.5 74-75 30.357166955325503 35.0 30.0 37.0 2.0 39.0 76-77 28.334322189793546 32.5 27.0 35.0 2.0 37.5 78-79 29.31439164241787 34.0 29.0 36.0 2.0 38.0 80-81 29.326434605322667 34.0 30.0 35.5 2.0 37.0 82-83 28.992439610681394 34.0 29.0 35.0 2.0 37.0 84-85 28.607597762276 34.0 29.0 35.0 2.0 36.5 86-87 28.100584152411862 34.0 28.5 35.0 2.0 36.0 88-89 27.59508581965294 34.0 27.0 35.0 2.0 35.5 90-91 27.195471473672423 33.5 26.0 35.0 2.0 35.0 92-93 26.91238222792269 33.0 26.0 35.0 2.0 35.0 94-95 26.537523467163425 33.0 25.0 35.0 2.0 35.0 96-97 26.071948032684617 33.0 24.5 35.0 2.0 35.0 98-99 25.430281256952895 33.0 21.0 35.0 2.0 35.0 100-101 22.770945215897903 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -7.370996484397097E-4 1101 2 0.06332074701499835 1101 3 0.10890930990537129 1101 4 0.17036217701992484 1101 5 0.15595397333853356 1101 6 0.12269526267925102 1101 7 0.050391260395969084 1101 8 0.1564818735009652 1101 9 0.40699986100815266 1101 10-11 0.3344590998405508 1101 12-13 0.3456362236630568 1101 14-15 0.562187378409071 1101 16-17 0.3922661930097249 1101 18-19 0.3118306165619984 1101 20-21 0.33562636701217485 1101 22-23 0.26235338029133715 1101 24-25 0.3144772416931687 1101 26-27 0.3844375865263956 1101 28-29 0.45973228678777645 1101 30-31 0.4650306137741822 1101 32-33 0.6952629835855433 1101 34-35 0.7770768996595265 1101 36-37 0.5530142071349715 1101 38-39 0.8840225436376556 1101 40-41 0.939198447077608 1101 42-43 0.844928945153633 1101 44-45 0.736690324242808 1101 46-47 0.8034723309215863 1101 48-49 0.8420727877078704 1101 50-51 0.8889601474260971 1101 52-53 0.7985718544860276 1101 54-55 1.1298639709281062 1101 56-57 1.04309157683754 1101 58-59 0.9870714346023775 1101 60-61 0.9253315499997967 1101 62-63 0.9151699047580166 1101 64-65 0.629017328145622 1101 66-67 0.6380959236951327 1101 68-69 0.7496356407185978 1101 70-71 0.5233970985234926 1101 72-73 0.6827677849210261 1101 74-75 0.684571755491973 1101 76-77 0.6848662176910132 1101 78-79 0.5128920194167073 1101 80-81 0.773551771629645 1101 82-83 0.7003334114986082 1101 84-85 0.7912543093015607 1101 86-87 0.9623249264893659 1101 88-89 1.023956397023838 1101 90-91 0.8913444535887471 1101 92-93 0.9351628134392129 1101 94-95 1.0570029674014592 1101 96-97 0.8213292446824987 1101 98-99 1.1098421586063587 1101 100-101 1.0624682399324001 1102 1 -0.15585449507916493 1102 2 -0.08834424617648295 1102 3 -0.06736035342809998 1102 4 0.07756683157337818 1102 5 -0.17157721519564006 1102 6 -0.04636241031911226 1102 7 0.04467393557214194 1102 8 -0.11603324508653401 1102 9 0.060496507180481274 1102 10-11 0.15315754878121624 1102 12-13 0.029531661265352227 1102 14-15 0.0636866287838842 1102 16-17 0.1328385673729926 1102 18-19 0.010106478630966365 1102 20-21 0.11245813461461296 1102 22-23 -0.20268932771245574 1102 24-25 -0.04021123975417851 1102 26-27 0.13949354478580034 1102 28-29 0.051028381859509864 1102 30-31 0.21469935738402768 1102 32-33 0.06461958968616699 1102 34-35 -0.10301745683194241 1102 36-37 -0.10892546523068347 1102 38-39 -0.16362335818207185 1102 40-41 -0.09292196060845725 1102 42-43 0.14465293636903453 1102 44-45 -0.05320830442450131 1102 46-47 -0.23109295524267282 1102 48-49 -0.21358745556498349 1102 50-51 -0.10795882428070769 1102 52-53 -0.06519351053788114 1102 54-55 -0.31964751186261253 1102 56-57 -0.4284730995398647 1102 58-59 -0.5247357352072086 1102 60-61 -0.4142612682699749 1102 62-63 -0.6220121922668653 1102 64-65 -0.2898810208450229 1102 66-67 -0.09795921387369333 1102 68-69 -0.4817096957874618 1102 70-71 -0.4854367872858987 1102 72-73 -0.20154235358770123 1102 74-75 -0.26591974187860856 1102 76-77 -0.40159972365024643 1102 78-79 -0.15034173068071865 1102 80-81 -0.17634942456124847 1102 82-83 0.08684428450247594 1102 84-85 0.049393588285919776 1102 86-87 0.033155336972196636 1102 88-89 0.013216354201212255 1102 90-91 0.05279010690887631 1102 92-93 0.0957075410753987 1102 94-95 0.024206292344494784 1102 96-97 0.0639648076349637 1102 98-99 0.1047426441434034 1102 100-101 0.2713660845880561 1103 1 0.05560754498513987 1103 2 -0.021665988919565393 1103 3 0.1442755454899114 1103 4 0.1316753194980933 1103 5 -0.031677285642338404 1103 6 -0.06829346286512816 1103 7 0.09509300314678626 1103 8 0.10068068710400269 1103 9 0.15582083296938976 1103 10-11 -0.12786496706929285 1103 12-13 0.09332882894657502 1103 14-15 0.17664520973436737 1103 16-17 0.11331783075335267 1103 18-19 8.362861019151069E-5 1103 20-21 0.03088446361878283 1103 22-23 0.010750639051153144 1103 24-25 0.17050456198350616 1103 26-27 -0.05349994340986797 1103 28-29 -0.06257483159400579 1103 30-31 -0.2535409200676142 1103 32-33 0.004589740153100763 1103 34-35 -0.058213804441273 1103 36-37 -0.02270840809659802 1103 38-39 0.019530170475292152 1103 40-41 0.006651746513881562 1103 42-43 -0.05866709036458673 1103 44-45 -0.16496906561017255 1103 46-47 -0.03179764807697438 1103 48-49 0.293387219747963 1103 50-51 0.128597596304175 1103 52-53 0.17950153651690215 1103 54-55 -0.07191332172876486 1103 56-57 -0.3963588855625204 1103 58-59 -0.8816442538394611 1103 60-61 -0.47105214993757016 1103 62-63 -0.25078289727580483 1103 64-65 -0.18476302261522193 1103 66-67 -0.2431584516042271 1103 68-69 -0.2128381074852257 1103 70-71 -0.28040526690151424 1103 72-73 -0.21729577205918815 1103 74-75 -0.24844241126680444 1103 76-77 -0.49345025922582764 1103 78-79 -0.3577948565274376 1103 80-81 -0.2716105289488162 1103 82-83 -0.5253353184671248 1103 84-85 -0.7883577167844358 1103 86-87 -0.5945056066523762 1103 88-89 -0.5167799428309934 1103 90-91 -0.563145524672926 1103 92-93 -0.46870264840817555 1103 94-95 -0.444242841522307 1103 96-97 -0.5909298835736472 1103 98-99 -0.5032270908686094 1103 100-101 -0.02542001031629937 1104 1 0.11781327727537061 1104 2 0.026739371004531165 1104 3 0.07669243444245666 1104 4 0.06292185094334002 1104 5 0.22992782723114402 1104 6 0.19304983006887255 1104 7 0.14726454220047458 1104 8 0.1383559050635057 1104 9 -0.014675416515920858 1104 10-11 0.22031639135101244 1104 12-13 0.17110277763881498 1104 14-15 0.1803151644417582 1104 16-17 0.16987205657193272 1104 18-19 0.08709064627968388 1104 20-21 0.164211232805485 1104 22-23 0.28772928170497636 1104 24-25 0.12051597464753883 1104 26-27 0.24988782758496342 1104 28-29 0.16449802148248693 1104 30-31 0.16465936994831765 1104 32-33 0.1303248499832037 1104 34-35 0.18598811467650478 1104 36-37 0.29301175338141405 1104 38-39 0.2684303029516997 1104 40-41 0.24328326515904308 1104 42-43 0.4009813100722184 1104 44-45 0.42363908168928077 1104 46-47 0.4202232885670725 1104 48-49 0.4457552356451302 1104 50-51 0.5077218197954707 1104 52-53 0.43933097313409775 1104 54-55 0.40027670136509386 1104 56-57 0.4866120770397444 1104 58-59 0.5458058884831729 1104 60-61 0.570758480399526 1104 62-63 0.17963341880576422 1104 64-65 0.4031402248471423 1104 66-67 0.3750342299690175 1104 68-69 0.5605466648880686 1104 70-71 0.44430785005367923 1104 72-73 0.4640156549266159 1104 74-75 0.4581368776570933 1104 76-77 0.3457665634885032 1104 78-79 0.41874853844920423 1104 80-81 0.4024124574992065 1104 82-83 0.4954245411794851 1104 84-85 0.42611533142623514 1104 86-87 0.6401891157737332 1104 88-89 0.7417974574088966 1104 90-91 0.7956764646215468 1104 92-93 0.5690162062079125 1104 94-95 0.6513986397072173 1104 96-97 0.8495519546261043 1104 98-99 0.5934258656827076 1104 100-101 1.4691847087083865 1105 1 -0.08317904241248542 1105 2 -0.06917260928749869 1105 3 -0.03462126445730007 1105 4 -0.19393278979497097 1105 5 -0.08806002182725337 1105 6 -0.055462663685872826 1105 7 -0.12202437466139315 1105 8 -0.18103131373779746 1105 9 -0.2504724624831596 1105 10-11 0.01437977609550245 1105 12-13 -0.10546415960150313 1105 14-15 -0.08337302916007161 1105 16-17 -0.14264159647520813 1105 18-19 -0.2234677822735236 1105 20-21 -0.07711083635842186 1105 22-23 -0.0925402537778055 1105 24-25 -0.078933145177011 1105 26-27 -0.38988368799519435 1105 28-29 -0.150499675072723 1105 30-31 -0.04913815617360484 1105 32-33 0.0018813081718960234 1105 34-35 -0.005730947248309803 1105 36-37 -0.1850838925672349 1105 38-39 0.11207831063417473 1105 40-41 -0.01690974837396908 1105 42-43 -0.05326557162587875 1105 44-45 -0.2717027689826139 1105 46-47 -0.33669299365141825 1105 48-49 -0.6146663920792648 1105 50-51 -0.4927085891362708 1105 52-53 -0.5438690199447507 1105 54-55 -0.5867072134528613 1105 56-57 -0.5982822997432606 1105 58-59 -0.5697405253885393 1105 60-61 -0.6313001287523932 1105 62-63 -0.7685066306371979 1105 64-65 -0.5428975256930286 1105 66-67 -0.6170053676527019 1105 68-69 -0.7939924190546463 1105 70-71 -0.6750624984477831 1105 72-73 -0.8712631700353732 1105 74-75 -0.9908585825019642 1105 76-77 -0.7998165540996496 1105 78-79 -0.9247340105233341 1105 80-81 -0.7345408528645656 1105 82-83 -0.6051765173236845 1105 84-85 -0.39076297098902657 1105 86-87 -0.3611496939184704 1105 88-89 -0.2238768895699117 1105 90-91 -0.3391918497110673 1105 92-93 -0.2085780890353135 1105 94-95 -0.04202151078450811 1105 96-97 -0.14030423688207705 1105 98-99 -0.020517869704473668 1105 100-101 0.20019642924105696 1106 1 -0.19293287219016975 1106 2 -0.23119352638219226 1106 3 -0.1781617809081908 1106 4 -0.0012351771139051948 1106 5 -0.00834165236031481 1106 6 -0.14529972580233874 1106 7 -0.009019482036237036 1106 8 -0.06296066243512399 1106 9 -0.1568969987097475 1106 10-11 -0.09726569774529992 1106 12-13 0.037579399510768496 1106 14-15 -0.2168140900197102 1106 16-17 0.01730492450280252 1106 18-19 -0.14815469915019008 1106 20-21 -0.25400841184784184 1106 22-23 -0.1246853781453865 1106 24-25 -0.29024261173338317 1106 26-27 -0.06605346144677782 1106 28-29 -0.1205058950548974 1106 30-31 -0.054874005714161456 1106 32-33 -0.1708001453802197 1106 34-35 -0.17007313604080565 1106 36-37 -0.24082647507818677 1106 38-39 -0.07605370688437318 1106 40-41 -0.1642079035400812 1106 42-43 -0.21141022147936184 1106 44-45 -0.1384123984127541 1106 46-47 -0.1721840836872559 1106 48-49 -3.4055478629824165E-4 1106 50-51 0.053309660419671445 1106 52-53 -0.0598999018356281 1106 54-55 0.02840093087636575 1106 56-57 -0.1340696404764472 1106 58-59 -0.04513632786242994 1106 60-61 -0.09564744742709053 1106 62-63 -0.18243682576509457 1106 64-65 -0.1954986965886505 1106 66-67 -0.1528920998536094 1106 68-69 -0.16449495514016377 1106 70-71 -0.13520331371431382 1106 72-73 -0.2904098141305589 1106 74-75 -0.23188609790764048 1106 76-77 -0.2709866161070984 1106 78-79 -0.2942554566371278 1106 80-81 -0.49717799705914345 1106 82-83 -0.2103551334097098 1106 84-85 -0.2427081581976651 1106 86-87 -0.2883149851354716 1106 88-89 0.0510751780932317 1106 90-91 -0.03568878140027465 1106 92-93 0.11027294559780643 1106 94-95 0.3456468936297554 1106 96-97 0.26802797428952374 1106 98-99 0.3676827599885648 1106 100-101 1.0026061914068904 1107 1 -0.07489281141883097 1107 2 -0.016739355980888604 1107 3 -0.3091507136592391 1107 4 0.02651876253430885 1107 5 -0.04374286397298022 1107 6 -0.013357267868691736 1107 7 0.026667787131209764 1107 8 0.07379391925337586 1107 9 -0.20245643193437957 1107 10-11 -0.06927019838416015 1107 12-13 -0.11183163976811272 1107 14-15 0.021783338005029407 1107 16-17 -0.020597701505550958 1107 18-19 0.04379309852448188 1107 20-21 -0.08076559983850728 1107 22-23 -0.12588681987546124 1107 24-25 -0.1028844486042999 1107 26-27 0.05740580077900859 1107 28-29 0.15696675466444532 1107 30-31 0.057508472307084446 1107 32-33 0.17301560812715877 1107 34-35 0.08361541720721988 1107 36-37 0.10201668274294917 1107 38-39 0.2615990955261367 1107 40-41 0.1639128254617077 1107 42-43 -0.07823453764287791 1107 44-45 0.049695871193314645 1107 46-47 -0.02288428901301387 1107 48-49 -0.03292886711029297 1107 50-51 0.1373946894386222 1107 52-53 0.10319740223083329 1107 54-55 0.011577568238031688 1107 56-57 -0.012609602716850077 1107 58-59 0.07346949118392132 1107 60-61 0.016204234909174886 1107 62-63 -0.1301423263340311 1107 64-65 -0.12309752947381725 1107 66-67 0.01710554811760545 1107 68-69 0.1610035796094742 1107 70-71 0.21866895205222292 1107 72-73 0.10339608628327213 1107 74-75 -0.00797295769494255 1107 76-77 -0.0907735350129606 1107 78-79 -0.029143671701550744 1107 80-81 -0.09117015272530793 1107 82-83 -0.03356520772110727 1107 84-85 -0.14375767158198016 1107 86-87 -0.21194397484375926 1107 88-89 -0.1463045347049814 1107 90-91 0.10859229239621726 1107 92-93 -0.05004642177001983 1107 94-95 0.016416896998876496 1107 96-97 -0.2500794689280532 1107 98-99 -0.5607265693018206 1107 100-101 -0.02626972953951423 1108 1 -0.26239799752149295 1108 2 -0.3102333016487435 1108 3 -0.25679789272296816 1108 4 -0.18876774307477717 1108 5 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-0.4741446270848755 2316 54-55 -0.50433247201412 2316 56-57 -0.3660243896842701 2316 58-59 -0.2897292253042636 2316 60-61 -0.3623092837359181 2316 62-63 -0.377780360814171 2316 64-65 -0.2849453307953027 2316 66-67 -0.5035103856562273 2316 68-69 -0.1605949242258724 2316 70-71 -0.36486943938090377 2316 72-73 -0.49921555645994786 2316 74-75 -0.20177944360669287 2316 76-77 -0.10863732327148412 2316 78-79 -0.27769022748494976 2316 80-81 -0.3232513025696129 2316 82-83 -0.5176228898051072 2316 84-85 -0.5134052754401104 2316 86-87 -0.8763540530696226 2316 88-89 -1.042778674600175 2316 90-91 -1.4841585240388078 2316 92-93 -1.378214379543241 2316 94-95 -1.2592713767157129 2316 96-97 -1.3697821346833514 2316 98-99 -1.9117717960049418 2316 100-101 -2.341732629212938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 8.0 5 65.0 6 103.0 7 264.0 8 476.0 9 816.0 10 1364.0 11 2155.0 12 3141.0 13 4348.0 14 4805.0 15 6215.0 16 7895.0 17 10760.0 18 9952.0 19 6112.0 20 5247.0 21 5448.0 22 5634.0 23 6160.0 24 6846.0 25 7555.0 26 8517.0 27 10004.0 28 12277.0 29 14484.0 30 17423.0 31 20814.0 32 25152.0 33 31319.0 34 40963.0 35 57895.0 36 81795.0 37 131132.0 38 126092.0 39 14421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.428422536938264 21.05400679062625 15.56983163567677 20.947739036758716 2 15.6556336461319 22.967209228939176 39.15275360169153 22.2244035232374 3 18.478818840042287 27.611344510319736 32.34389519284882 21.565941456789155 4 12.475296766003115 19.212026821277284 37.84193711163753 30.470739301082073 5 12.067684316300282 38.9770861599359 34.555007321900405 14.400222201863414 6 27.79617341633911 38.23907931646751 18.384150375983584 15.580596891209794 7 24.69014619079404 35.796167599536396 22.264248621781306 17.249437587888252 8 25.05035169843368 34.1628093993715 21.265503596965374 19.52133530522945 9 25.712303846506213 17.18705237885418 22.275445966992553 34.82519780764706 10-11 23.421501520366807 27.82402135929954 28.21345630054256 20.54102081979109 12-13 25.43564216774786 26.24526839454791 28.40446555943827 19.914623878265953 14-15 22.270606780656777 27.145894349258793 28.42871934176965 22.154779528314783 16-17 19.792146109956203 30.136414567659404 29.075404295666452 20.996035026717944 18-19 19.411644092592002 28.21502424156053 32.59224735407802 19.781084311769444 20-21 20.947542618986333 27.07009101842036 32.36338148191774 19.618984880675562 22-23 20.46423902405684 27.46614257274275 32.16102945774311 19.908588945457296 24-25 20.30943936201308 27.77857758815001 32.50807444925785 19.40390860057906 26-27 20.164993848722194 27.707143916633463 32.78551090506674 19.34235132957761 28-29 19.589101056767632 28.09704753636592 32.712244224278464 19.601607182587983 30-31 20.15325820932637 27.692707038185855 32.445398400088415 19.70863635239936 32-33 19.583138833992813 28.131657512473407 32.245809357199676 20.039394296334105 34-35 20.292956550256285 27.969831757950175 32.1787552691531 19.558456422640432 36-37 20.22855131568996 27.712974221436177 32.078633055437585 19.97984140743628 38-39 19.841619581579003 27.814233876538903 32.28153610099115 20.062610440890943 40-41 19.975829444884372 27.81009971444714 32.20072031744577 20.013350523222712 42-43 19.847157841159042 27.89340313755431 31.627928959662615 20.631510061624038 44-45 19.89656097601765 27.982924490094184 31.42442065493931 20.696093878948858 46-47 20.42517176214987 28.050109684920344 30.827534005233755 20.697184547696036 48-49 20.30343395447182 28.227457675427754 30.463585307898356 21.00552306220207 50-51 19.849067743556223 28.54894028221816 30.441882937703905 21.16010903652171 52-53 20.146641596247 28.86384755323465 30.023645303004525 20.965865547513825 54-55 19.687768572687496 28.41006423204389 30.134237264474024 21.76792993079459 56-57 20.19695693966376 28.768161148702198 29.451999016960347 21.5828828946737 58-59 20.14348629376517 28.457460538360806 29.84353493688392 21.555518230990103 60-61 20.069307808980348 28.84803034070765 29.764548314057738 21.318113536254266 62-63 19.793488961889764 28.83876259156011 29.76348880192769 21.604259644622438 64-65 19.527006687868912 28.98818171109977 29.486899833203182 21.99791176782814 66-67 19.665616963169732 28.885799482152734 29.130108215680984 22.318475338996546 68-69 19.737412310806565 28.718501692706816 29.13178973207534 22.412296264411275 70-71 19.955733101431402 28.64652939662356 28.876007602738035 22.521729899207006 72-73 19.878268861346328 28.390068972738096 28.979311086971382 22.75235107894419 74-75 19.575940540642726 28.856067579613853 28.58871278518693 22.97927909455649 76-77 19.53564132255104 28.570700759326368 28.86844869033851 23.02520922778408 78-79 19.469190038403354 28.601550680786687 28.778002443849644 23.151256836960314 80-81 19.564452355296126 28.689198051952776 28.537961181567134 23.208388411183968 82-83 19.52918798888409 28.47892063408966 28.466923478506185 23.52496789852006 84-85 19.366681063253367 28.351896204972494 28.35538628659678 23.926036445177363 86-87 19.371610325001022 28.31576479223586 28.304550829833218 24.008074052929903 88-89 19.368020547100844 28.226805913491802 28.119577007496538 24.285596531910812 90-91 19.30696065242673 28.070317427332945 28.17545816791166 24.447263752328663 92-93 19.46513044907171 28.270533677106375 27.94202974422064 24.322306129601273 94-95 19.245836987012535 27.82082211781061 28.119005966585377 24.814334928591474 96-97 19.185705789027473 27.810497293005444 28.083305339970305 24.92049157799678 98-99 19.13407851694259 27.87661934922829 27.744213896246766 25.24508823758235 100-101 19.12671769490234 27.625412904806673 28.2486146830149 24.99925471727609 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2034.0 1 1358.5 2 784.5 3 872.0 4 772.0 5 774.0 6 1095.0 7 1911.5 8 2691.5 9 2808.0 10 2306.5 11 1842.5 12 1836.0 13 1862.5 14 1952.0 15 2033.5 16 2055.5 17 2151.0 18 2247.5 19 2315.5 20 2514.5 21 2920.5 22 3323.5 23 3724.0 24 4155.0 25 4763.0 26 5780.0 27 6861.5 28 7752.0 29 8773.0 30 9978.5 31 11190.0 32 12592.0 33 14037.0 34 15586.0 35 17090.0 36 18574.0 37 20019.5 38 20434.5 39 20247.5 40 20578.0 41 21251.5 42 21827.0 43 22583.0 44 23431.0 45 24103.5 46 24268.5 47 25289.0 48 28002.5 49 28307.0 50 25775.0 51 22919.0 52 20117.5 53 18222.0 54 16894.5 55 15440.0 56 13916.0 57 12223.0 58 11036.5 59 9887.5 60 8325.5 61 6962.0 62 5564.0 63 4128.0 64 2950.0 65 2181.0 66 1564.5 67 1170.5 68 855.5 69 596.5 70 477.5 71 373.0 72 312.0 73 290.0 74 249.0 75 215.0 76 152.5 77 70.5 78 45.5 79 35.5 80 23.5 81 18.0 82 13.0 83 8.0 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3782375960317772 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.454200676784995E-4 16-17 0.0023994311166952416 18-19 1.454200676784995E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.454200676784995E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007634553553121223 36-37 0.01672330778302744 38-39 0.01134276527892296 40-41 0.007198293350085725 42-43 0.003708211725801737 44-45 0.0016723307783027444 46-47 0.0016723307783027444 48-49 1.454200676784995E-4 50-51 5.089702368747483E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.1813010151774926E-4 60-61 8.72520406070997E-4 62-63 0.0 64-65 0.0 66-67 0.0012360705752672457 68-69 0.0010179404737494966 70-71 0.002326721082855992 72-73 0.0 74-75 0.0 76-77 0.09779499551379092 78-79 0.03242867509230539 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14818304896439097 88-89 0.32188731980635865 90-91 0.2785521396381658 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.6355016919624873E-4 100-101 6.543903045532477E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 687663.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.617750689710064 #Duplication Level Percentage of deduplicated Percentage of total 1 82.34574929793669 51.563055998955676 2 10.969007529068083 13.737091575374757 3 3.0413603113053336 5.713294251926891 4 1.149212595407426 2.8784443115478733 5 0.5936173561581454 1.8585491806497785 6 0.3735140236347886 1.4033164806644196 7 0.2595644294302506 1.1377338510986192 8 0.1826964727767957 0.9152033747381436 9 0.13488606170012185 0.760163560474957 >10 0.8577257653288817 9.998837509443922 >50 0.06479673083714564 2.79351287273786 >100 0.02345676723404619 2.655928423507604 >500 0.0020902069810799966 0.8551091895902372 >1k 0.001857961760959997 2.0923998333034577 >5k 4.6449044023999926E-4 1.6373595859857961 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6135 0.8921521152075944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5124 0.7451324267846314 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3794 0.5517237367722271 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2324 0.3379562372848328 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1855 0.26975422554361655 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1687 0.24532365417362867 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1544 0.22452858449560323 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1083 0.15748993329581495 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1063 0.15458153194224497 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1038 0.1509460302502825 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 971 0.141202885715823 RNA PCR Primer, Index 17 (95% over 21bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 838 0.12186201671458258 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.454200676784995E-4 0.0 12-13 0.0 0.0 0.0 1.454200676784995E-4 0.0 14-15 0.0 0.0 0.0 2.1813010151774926E-4 0.0 16-17 0.0 0.0 0.0 2.90840135356999E-4 0.0 18-19 0.0 0.0 0.0 3.6355016919624873E-4 0.0 20-21 0.0 0.0 0.0 7.271003383924975E-4 0.0 22-23 0.0 7.271003383924975E-5 0.0 0.0015996207444634945 0.0 24-25 0.0 1.454200676784995E-4 0.0 0.004798862233390483 0.0 26-27 0.0 1.454200676784995E-4 0.0 0.010179404737494964 0.0 28-29 0.0 1.454200676784995E-4 0.0 0.030247374077127895 0.0 30-31 0.0 1.454200676784995E-4 0.0 0.08259859844138771 0.0 32-33 0.0 1.454200676784995E-4 0.0 0.18715562710222886 0.0 34-35 0.0 1.454200676784995E-4 0.0 0.3381743673863506 0.0 36-37 0.0 1.454200676784995E-4 0.0 0.5736821669916805 0.0 38-39 0.0 1.454200676784995E-4 0.0 0.9028404901819641 0.0 40-41 0.0 1.454200676784995E-4 0.0 1.2575200352498244 0.0 42-43 0.0 1.454200676784995E-4 0.0 1.5940220718578724 0.0 44-45 0.0 1.454200676784995E-4 0.0 1.9851293438791968 0.0 46-47 0.0 1.454200676784995E-4 0.0 2.426988219520317 0.0 48-49 0.0 1.454200676784995E-4 0.0 2.872991567090275 0.0 50-51 0.0 1.454200676784995E-4 0.0 3.362839065065301 0.0 52-53 0.0 1.454200676784995E-4 0.0 3.8340727943774784 0.0 54-55 0.0 1.454200676784995E-4 0.0 4.327628504078305 0.0 56-57 0.0 1.454200676784995E-4 0.0 4.907272893844805 0.0 58-59 0.0 1.454200676784995E-4 0.0 5.522836040327893 0.0 60-61 0.0 1.454200676784995E-4 0.0 6.1790440957271215 0.0 62-63 0.0 1.454200676784995E-4 0.0 6.836851771870815 0.0 64-65 0.0 1.454200676784995E-4 0.0 7.5040390423797705 0.0 66-67 0.0 1.454200676784995E-4 0.0 8.246626617980027 0.0 68-69 0.0 4.362602030354985E-4 0.0 9.052108372851237 0.0 70-71 0.0 4.362602030354985E-4 0.0 9.935971544201156 0.0 72-73 0.0 4.362602030354985E-4 0.0 10.816126503825275 0.0 74-75 0.0 4.362602030354985E-4 0.0 11.737944312839282 0.0 76-77 0.0 4.362602030354985E-4 0.0 12.664924534255878 0.0 78-79 0.0 4.362602030354985E-4 0.0 13.6860642494943 0.0 80-81 0.0 4.362602030354985E-4 0.0 14.77765998752296 0.0 82-83 0.0 4.362602030354985E-4 0.0 15.888669304586696 0.0 84-85 0.0 4.362602030354985E-4 0.0 17.006731494932836 0.0 86-87 0.0 4.362602030354985E-4 0.0 18.132864499035136 0.0 88-89 0.0 4.362602030354985E-4 0.0 19.275938941021984 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5840 0.0 26.835394 1 GTATCAA 7935 0.0 25.573349 1 GAGTACT 4920 0.0 22.952406 12-13 CTACACG 125 2.2714619E-4 22.774958 4 GTACATG 3900 0.0 22.351744 1 GTGTAGC 300 7.2759576E-12 22.229605 1 TACACGG 150 3.0567506E-5 22.14232 5 TACATGG 3970 0.0 21.393354 2 AGTACTT 4970 0.0 21.146265 12-13 ATCAACG 9515 0.0 21.143332 3 TATCAAC 9550 0.0 21.115526 2 TCAACGC 9545 0.0 21.076878 4 ACATGGG 3905 0.0 21.020422 3 CAACGCA 9590 0.0 20.977976 5 GCGGTAT 115 0.0036611292 20.71081 1 GTACTTT 5400 0.0 20.692526 14-15 AACGCAG 9770 0.0 20.591484 6 AGAGTAC 7860 0.0 19.951027 10-11 CATGGGG 1055 0.0 19.338926 4 CGCAGAG 10495 0.0 19.033384 8 >>END_MODULE