FastQCFastQC Report
Thu 26 May 2016
SRR938776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688702
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68190.9901234496197194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50790.7374742631791399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35770.519382839021812No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21960.31886069736983486No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17160.24916437007588185No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16310.2368223121175777No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10910.1584139439118806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.15826874323001822No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.14839509686337488No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.14069946072466757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.024.9498561
GTATCAA78800.024.3055321
TAATACC2252.8521754E-923.1906914
GAGTACT44450.022.42189812-13
GTACATG34850.022.2285481
GTACACG1503.0183997E-522.1785451
TACATGG36900.020.9538962
AGTACTT45150.020.86544412-13
ATCAACG91250.020.7936463
TATCAAC91550.020.777322
TCAACGC91650.020.7028924
GTATAGT1150.00371023520.663241
CAACGCA92200.020.5793935
AACGCAG93650.020.3620626
ACATGGG36500.020.0138843
GTACTTT50600.019.6498114-15
AGAGTAC72100.018.8238410-11
CATGGGG11450.018.6427754
CGCAGAG101200.018.5148458
ACGCAGA102300.018.5012367
Produced by FastQC (version 0.11.5)