##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938776_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688702 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38923075582763 33.0 31.0 34.0 30.0 34.0 2 31.424788950808914 33.0 31.0 34.0 28.0 34.0 3 31.411893678252714 33.0 31.0 34.0 28.0 34.0 4 34.99422827289597 37.0 35.0 37.0 32.0 37.0 5 35.03410328414902 37.0 35.0 37.0 32.0 37.0 6 35.109375317626494 37.0 35.0 37.0 32.0 37.0 7 35.02790611904714 37.0 35.0 37.0 32.0 37.0 8 35.00934221187103 37.0 35.0 37.0 32.0 37.0 9 36.5365339435634 39.0 37.0 39.0 32.0 39.0 10-11 36.423734793858586 39.0 37.0 39.0 32.0 39.0 12-13 36.30889847858725 39.0 37.0 39.0 32.0 39.0 14-15 37.382627754819936 40.0 37.0 41.0 32.0 41.0 16-17 37.295444183405884 40.0 37.0 41.0 32.0 41.0 18-19 37.29660361085055 40.0 37.0 41.0 32.0 41.0 20-21 37.21581177345209 40.0 37.0 41.0 31.0 41.0 22-23 37.16725811744412 40.0 37.0 41.0 31.0 41.0 24-25 37.080761345255276 40.0 36.5 41.0 31.0 41.0 26-27 36.9113317516139 40.0 36.0 41.0 30.5 41.0 28-29 36.70933001501375 39.5 36.0 41.0 30.0 41.0 30-31 36.41028921071813 39.0 35.5 41.0 30.0 41.0 32-33 36.125159357748345 39.0 35.0 41.0 30.0 41.0 34-35 35.76615865788106 39.0 35.0 41.0 28.0 41.0 36-37 35.488645306678364 38.0 35.0 40.0 27.0 41.0 38-39 35.277925721139184 38.0 35.0 40.0 26.0 41.0 40-41 35.075948668654945 38.0 35.0 40.0 25.0 41.0 42-43 34.939431713571324 38.0 35.0 41.0 24.5 41.0 44-45 34.63938031252995 38.0 34.5 41.0 23.5 41.0 46-47 34.156967745120525 38.0 34.0 40.5 19.0 41.0 48-49 33.711286594201844 38.0 33.0 40.0 13.5 41.0 50-51 32.51272829177206 37.0 31.5 39.5 8.5 40.5 52-53 32.5609370961606 37.5 32.5 39.5 7.5 40.5 54-55 33.03425574486498 38.0 33.0 40.0 4.5 41.0 56-57 32.92109794947597 38.0 33.0 40.0 2.0 41.0 58-59 32.707796695813286 38.0 33.0 40.0 2.0 41.0 60-61 32.38564865500609 37.0 32.0 40.0 2.0 41.0 62-63 31.982109823987734 37.0 31.5 40.0 2.0 41.0 64-65 31.58054862625635 36.0 31.0 39.5 2.0 41.0 66-67 31.17304218660611 36.0 31.0 39.0 2.0 41.0 68-69 30.7169588588388 35.0 30.0 39.0 2.0 40.5 70-71 30.254618833690042 35.0 29.5 38.0 2.0 40.0 72-73 29.787249056921574 35.0 29.0 37.5 2.0 40.0 74-75 29.288362020148046 35.0 29.0 37.0 2.0 39.0 76-77 28.783590725742048 34.5 28.0 36.0 2.0 39.0 78-79 28.283613957851145 34.0 27.0 36.0 2.0 38.5 80-81 27.74813126722443 34.0 26.0 35.5 2.0 37.0 82-83 27.271248667783745 34.0 26.0 35.0 2.0 37.0 84-85 26.853513566099707 34.0 25.0 35.0 2.0 36.5 86-87 26.358297202563662 34.0 23.5 35.0 2.0 36.0 88-89 25.837359264239105 33.0 20.5 35.0 2.0 36.0 90-91 25.301268908758793 33.0 18.0 35.0 2.0 35.0 92-93 24.759883084410966 33.0 8.0 35.0 2.0 35.0 94-95 24.184545710626658 32.0 2.0 35.0 2.0 35.0 96-97 23.530140757540998 32.0 2.0 35.0 2.0 35.0 98-99 22.747694213172025 31.5 2.0 34.5 2.0 35.0 100-101 20.53308978338962 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06869948154519889 1101 2 0.18544897440466102 1101 3 0.38682746139404856 1101 4 0.4399358031817471 1101 5 0.04705792972519163 1101 6 0.5477383908905153 1101 7 0.4727059766657433 1101 8 0.5106613941035718 1101 9 0.8007392782885745 1101 10-11 0.5317936649393218 1101 12-13 0.40125252918141285 1101 14-15 0.5413311553787992 1101 16-17 0.6583432322418119 1101 18-19 0.7011732391730376 1101 20-21 0.584962980495817 1101 22-23 0.7130445763881781 1101 24-25 0.6845628940897157 1101 26-27 0.642181173010222 1101 28-29 0.6056224785602353 1101 30-31 0.6200620501023195 1101 32-33 0.8455752537886738 1101 34-35 0.9377815904173019 1101 36-37 0.4527004622387736 1101 38-39 0.6723872893264726 1101 40-41 0.7605182181659913 1101 42-43 0.6383194371425986 1101 44-45 0.5681799418999631 1101 46-47 0.42686942321724786 1101 48-49 0.43763866169440746 1101 50-51 0.47409720415625856 1101 52-53 0.7233792867404532 1101 54-55 0.7040716978092476 1101 56-57 0.6507386761511427 1101 58-59 0.5912185550220102 1101 60-61 0.3996975208868747 1101 62-63 0.573515334244199 1101 64-65 0.2129295806154765 1101 66-67 0.2401343900423747 1101 68-69 0.22798286736427897 1101 70-71 -0.06397667370276494 1101 72-73 -0.04037340560142155 1101 74-75 -0.33253885585144616 1101 76-77 -0.2767641968685126 1101 78-79 -0.38088183097106665 1101 80-81 -0.33975210811102485 1101 82-83 -0.18765136051983689 1101 84-85 -0.07061595753157945 1101 86-87 -0.18008560970049814 1101 88-89 -0.20466171036196457 1101 90-91 -0.03360385354147866 1101 92-93 -0.1489205766985151 1101 94-95 0.0435865881215598 1101 96-97 -0.009519300650726592 1101 98-99 0.42421765374140463 1101 100-101 0.579005235701306 1102 1 0.08184677841794752 1102 2 0.23255456535254382 1102 3 0.3161557053626822 1102 4 0.33565507703369235 1102 5 0.30211888400335596 1102 6 0.32827179374582016 1102 7 0.03357000996498272 1102 8 0.14372354719005642 1102 9 0.39347646073249365 1102 10-11 0.3871779409415481 1102 12-13 0.3556944705378484 1102 14-15 0.4208720875525458 1102 16-17 0.38056508308599035 1102 18-19 0.7200399865030178 1102 20-21 0.6886910961162016 1102 22-23 0.5036998877998116 1102 24-25 0.5090711709921507 1102 26-27 0.4795966937899365 1102 28-29 0.6709497331777641 1102 30-31 0.6704253692415847 1102 32-33 0.6042382037038863 1102 34-35 0.3862272618947955 1102 36-37 0.5298653919703327 1102 38-39 0.5710155673876116 1102 40-41 0.679708955700498 1102 42-43 0.5579402304506615 1102 44-45 0.3832916115636422 1102 46-47 0.5196166328683205 1102 48-49 0.22374941999637343 1102 50-51 0.30904157543923105 1102 52-53 0.31851408582864593 1102 54-55 0.06117619690568432 1102 56-57 0.14204174514501489 1102 58-59 0.20884549389392504 1102 60-61 0.05227373323192097 1102 62-63 0.1601704584812964 1102 64-65 0.08170686297494356 1102 66-67 0.09784386397052458 1102 68-69 0.20340952502874288 1102 70-71 0.0443806692210309 1102 72-73 -0.07658610717613712 1102 74-75 -0.14751794355385783 1102 76-77 -0.07701108899136244 1102 78-79 -0.10714018976236517 1102 80-81 -3.96879813802542E-4 1102 82-83 -0.0768652051961709 1102 84-85 -0.21446515724903392 1102 86-87 -0.3107378906673013 1102 88-89 -0.05501344250629003 1102 90-91 -0.18183271451759353 1102 92-93 -0.2964085384792554 1102 94-95 -0.4583865434197989 1102 96-97 -0.4961864575364068 1102 98-99 -0.4494350795638553 1102 100-101 -0.09775102963196503 1103 1 0.2521585271476887 1103 2 0.5379227771181654 1103 3 0.5510523305754731 1103 4 0.47732596705747454 1103 5 0.46152787390628447 1103 6 0.3790820285644756 1103 7 0.5664992005098597 1103 8 0.5058471810964704 1103 9 0.5437954866314243 1103 10-11 0.45880607759638536 1103 12-13 0.5889050016412511 1103 14-15 0.6422214826378578 1103 16-17 0.6433813475591137 1103 18-19 0.6185852024140317 1103 20-21 0.7909255769031773 1103 22-23 0.868903323663254 1103 24-25 0.8864476215528825 1103 26-27 0.7396482837214506 1103 28-29 0.720047883997097 1103 30-31 0.9460643956603079 1103 32-33 0.8960137030346473 1103 34-35 1.016183209031368 1103 36-37 1.0133358219730582 1103 38-39 0.9497304972798872 1103 40-41 0.9755161367070855 1103 42-43 1.1636930925872875 1103 44-45 1.1366118200025639 1103 46-47 1.115194453096251 1103 48-49 1.0587493550216536 1103 50-51 1.1626762615463306 1103 52-53 1.2970886703152829 1103 54-55 1.4006106569007457 1103 56-57 1.2747981029218423 1103 58-59 1.3939454899954384 1103 60-61 1.2131316594986075 1103 62-63 1.2098273919341835 1103 64-65 1.028235913174413 1103 66-67 1.1952767125924098 1103 68-69 1.2617080968842878 1103 70-71 1.2141498789984304 1103 72-73 1.395195369829306 1103 74-75 1.4802451066196234 1103 76-77 1.327459821824327 1103 78-79 1.368075201450452 1103 80-81 1.3616033021154301 1103 82-83 1.1749768282528166 1103 84-85 1.131531749954064 1103 86-87 0.9699204332446989 1103 88-89 1.14632373733075 1103 90-91 1.0526838085549315 1103 92-93 0.7833771748782468 1103 94-95 0.44489857866770777 1103 96-97 0.9092265867893481 1103 98-99 1.0432715075737455 1103 100-101 1.0982094917252283 1104 1 -0.2708243435951374 1104 2 -0.07090750579950722 1104 3 0.14097674060497312 1104 4 0.3554392346895412 1104 5 0.15968337806289412 1104 6 0.33663599745693773 1104 7 0.25871309833770084 1104 8 0.4704489510079739 1104 9 0.4710925166662818 1104 10-11 0.4977512539171087 1104 12-13 0.49812580480563184 1104 14-15 0.4482288740296454 1104 16-17 0.5172332515548206 1104 18-19 0.41373596334189244 1104 20-21 0.4108764685631314 1104 22-23 0.3585851250845451 1104 24-25 0.3247395563879607 1104 26-27 0.13644115714595983 1104 28-29 0.41970136013010517 1104 30-31 0.5568766107313721 1104 32-33 0.2678522488914439 1104 34-35 0.3561453302434856 1104 36-37 0.7099137416566208 1104 38-39 0.27168762385537804 1104 40-41 0.3876553663933251 1104 42-43 0.5557574027240619 1104 44-45 0.5076877619441049 1104 46-47 0.7260841855982676 1104 48-49 0.5738966800417629 1104 50-51 0.6993922879609187 1104 52-53 0.9402507948795105 1104 54-55 0.7396132380216898 1104 56-57 0.51363140273412 1104 58-59 0.6300342537390762 1104 60-61 0.5927952757496158 1104 62-63 0.4092944072197824 1104 64-65 0.48053545728811997 1104 66-67 0.33204469663638747 1104 68-69 0.3828091796142452 1104 70-71 0.520999486802129 1104 72-73 0.41254251383574214 1104 74-75 0.278932338451213 1104 76-77 0.36530927878330033 1104 78-79 0.1161037446255655 1104 80-81 0.3622779132712317 1104 82-83 0.255178038362768 1104 84-85 0.13426790575991276 1104 86-87 0.1264846930998864 1104 88-89 0.2557573991703883 1104 90-91 0.1330107226069579 1104 92-93 -0.22996839363878152 1104 94-95 -0.018201029135990865 1104 96-97 -0.40007424788482737 1104 98-99 -0.37214649993496707 1104 100-101 0.3746408406123294 1105 1 0.12105871455510808 1105 2 0.2024549629546044 1105 3 0.2546028259884743 1105 4 0.20177729876006367 1105 5 0.01471273275306828 1105 6 0.14472457132879413 1105 7 0.05804665642216378 1105 8 0.20633896547975183 1105 9 0.28244751929752 1105 10-11 0.023145733261934254 1105 12-13 0.16603339920110471 1105 14-15 0.24491989330342534 1105 16-17 0.2925794231111922 1105 18-19 0.3970122181109943 1105 20-21 0.5173694464607763 1105 22-23 0.4305791472526863 1105 24-25 0.32715494581040616 1105 26-27 0.29742120911280523 1105 28-29 0.3268178168463294 1105 30-31 0.37552300594252586 1105 32-33 0.23412827342769305 1105 34-35 0.3854547961032395 1105 36-37 0.29690373474963394 1105 38-39 0.5996276153038664 1105 40-41 0.6586898108743142 1105 42-43 0.8869299046974817 1105 44-45 0.8697793041753883 1105 46-47 0.6640847365126206 1105 48-49 0.8048880791997703 1105 50-51 0.6944042518472457 1105 52-53 0.8591625745795497 1105 54-55 0.6601315086439712 1105 56-57 0.7314830834340285 1105 58-59 0.7204335433062354 1105 60-61 0.41811225706786104 1105 62-63 0.4579639901693966 1105 64-65 0.5281772149515831 1105 66-67 0.23238572906632982 1105 68-69 0.07593035610418042 1105 70-71 -0.004357990832392744 1105 72-73 0.048749920755888354 1105 74-75 0.1989685590142969 1105 76-77 -0.03205825509382265 1105 78-79 -0.08517219768465267 1105 80-81 -0.07582439234644767 1105 82-83 -0.13619419062025884 1105 84-85 -0.2100556207497597 1105 86-87 -0.2636387281604726 1105 88-89 -0.1582773495468892 1105 90-91 0.02630909617622379 1105 92-93 -0.008877007636939993 1105 94-95 0.16666259900504343 1105 96-97 0.12286784032724896 1105 98-99 -0.08187630522374789 1105 100-101 0.28786771865469163 1106 1 0.13710183272502618 1106 2 0.11385347425419567 1106 3 0.24639604440244156 1106 4 0.2617751412232323 1106 5 -0.016858544325550895 1106 6 -0.07045308961632912 1106 7 0.017733284375289315 1106 8 -0.08580983942803044 1106 9 0.047255651175937885 1106 10-11 0.17460583613166136 1106 12-13 0.185979420072222 1106 14-15 0.28198474763916437 1106 16-17 0.1606012671538508 1106 18-19 0.13686619764696673 1106 20-21 -0.004205921796156531 1106 22-23 0.13087387492472402 1106 24-25 -0.028358273173473947 1106 26-27 0.07713568150345651 1106 28-29 -0.0933054884186788 1106 30-31 0.07220793025572902 1106 32-33 0.10952950403784456 1106 34-35 0.17441105713263738 1106 36-37 0.17272120713418815 1106 38-39 0.373846475025303 1106 40-41 0.33994427605961164 1106 42-43 0.5037849487539745 1106 44-45 0.6146403974766415 1106 46-47 0.48021069595499455 1106 48-49 0.596745820177297 1106 50-51 0.381273401309592 1106 52-53 0.3463120002880231 1106 54-55 0.36234970075024364 1106 56-57 0.3388235936304298 1106 58-59 0.3938272469624451 1106 60-61 0.37067494267200374 1106 62-63 0.3005726964411153 1106 64-65 0.10496008329502615 1106 66-67 0.3185264045381899 1106 68-69 0.2733612589519261 1106 70-71 0.2996053636081655 1106 72-73 0.3943303286931794 1106 74-75 0.4070059943136357 1106 76-77 0.4837304365636328 1106 78-79 0.7986820993756005 1106 80-81 0.5715282489060662 1106 82-83 0.48433171915806383 1106 84-85 0.6851705237115802 1106 86-87 0.5375554481184288 1106 88-89 0.6966517061643778 1106 90-91 0.4257312426811133 1106 92-93 0.4948050528514365 1106 94-95 0.5468551896979186 1106 96-97 0.750367071959186 1106 98-99 0.7178635691099124 1106 100-101 1.357083942517459 1107 1 0.025806234043020737 1107 2 0.19865229353374048 1107 3 0.23139899025236232 1107 4 0.39795870834001335 1107 5 0.2850900808453005 1107 6 0.14135054419369908 1107 7 0.1689119219048223 1107 8 0.06212737131826884 1107 9 -0.082149485513483 1107 10-11 0.17782742554168607 1107 12-13 0.33065040731877815 1107 14-15 0.3806290615536909 1107 16-17 0.6451142298853725 1107 18-19 0.48027394096722986 1107 20-21 0.41186016839644424 1107 22-23 0.339843739971073 1107 24-25 0.3921861040222723 1107 26-27 0.37131301571756836 1107 28-29 0.43184334147386494 1107 30-31 0.5398511908057415 1107 32-33 0.6716730512378959 1107 34-35 0.8344718785189045 1107 36-37 0.7714250331683559 1107 38-39 0.49578085696218466 1107 40-41 0.24054415822561026 1107 42-43 0.3966274261244891 1107 44-45 1.014682849455319 1107 46-47 0.8821228203591289 1107 48-49 1.0189357074811838 1107 50-51 0.6774473671377379 1107 52-53 0.6023599194467621 1107 54-55 0.671978364961042 1107 56-57 0.8645397723969026 1107 58-59 0.8019633472125989 1107 60-61 0.5274844185884362 1107 62-63 0.3580137943206907 1107 64-65 0.5965599476073891 1107 66-67 0.3836148097919221 1107 68-69 0.5084212372498378 1107 70-71 0.5206859450018975 1107 72-73 0.5770107148871588 1107 74-75 0.831582317615208 1107 76-77 0.5663947544853123 1107 78-79 0.6009044802968084 1107 80-81 0.6713419759715791 1107 82-83 0.5656034534800938 1107 84-85 0.6621661138635133 1107 86-87 0.6326012625318675 1107 88-89 0.8012700450224166 1107 90-91 0.7889892535083511 1107 92-93 0.57540529209016 1107 94-95 0.5956967862890643 1107 96-97 0.579337175054011 1107 98-99 0.7658719960260534 1107 100-101 1.1036525728856965 1108 1 0.037710583900679495 1108 2 0.07107694763049821 1108 3 -0.15187218577539596 1108 4 0.07979262286578859 1108 5 0.08345812708687816 1108 6 0.21018939957629357 1108 7 0.22551977708839388 1108 8 -0.05255718396135478 1108 9 0.05941341739132611 1108 10-11 0.21813521236664002 1108 12-13 0.16621873564779577 1108 14-15 0.2934036857976352 1108 16-17 0.4252958561281588 1108 18-19 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1698.0 9 2052.0 10 2559.0 11 3284.0 12 4587.0 13 5910.0 14 6228.0 15 7429.0 16 8697.0 17 11260.0 18 9859.0 19 6487.0 20 6360.0 21 6644.0 22 7214.0 23 7791.0 24 8724.0 25 9739.0 26 10942.0 27 12440.0 28 14488.0 29 16377.0 30 18932.0 31 22465.0 32 26654.0 33 32378.0 34 41654.0 35 57385.0 36 77289.0 37 111117.0 38 103008.0 39 16645.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.085874330744886 20.041760645105867 15.139689940314893 20.732675083834348 2 15.470272188591757 22.442686804220276 39.71155048884571 22.375490518342257 3 18.65229118354782 27.65335901561595 31.841035593432398 21.85331420740383 4 12.506235737795043 18.351758150818316 38.70884231255336 30.43316379883328 5 12.24035953800298 38.38414563152012 34.55663189269747 14.818862937779434 6 27.931445863764658 37.55303052346728 18.984490936072397 15.531032676695666 7 24.723824050813466 35.52343165536735 22.880613394424376 16.872130899394815 8 24.75470324951785 33.48902878352316 21.877078708926167 19.879189258032824 9 26.333653752466766 17.026326237091737 22.6555717300161 33.9844482804254 10-11 23.372230063001062 27.42059392687221 28.553085306057124 20.654090704069606 12-13 25.535761575913135 26.103025699676678 28.671829301298224 19.689383423111956 14-15 22.49727996834872 26.78220255653884 28.986082747144692 21.734434727967745 16-17 19.763418814371065 29.911713440729475 29.415893328052352 20.90897441684711 18-19 19.414112130354486 28.03411675842768 33.04742610728106 19.504345003936773 20-21 20.955783396695285 26.90751482931556 32.82592849629213 19.31077327769703 22-23 20.53454203528874 27.09455938563473 32.52156420938025 19.84933436969628 24-25 20.224978296100137 27.3322868888069 32.90966156470382 19.53307325038914 26-27 20.00030595653669 27.21803919740316 33.1660528110121 19.61560203504805 28-29 19.589619223444505 27.826147118049104 32.962934641308266 19.621299017198123 30-31 19.94903401251579 27.441398215462353 32.75569453899444 19.853873233027418 32-33 19.722254350187058 27.68196370000677 32.55811585125892 20.03766609854726 34-35 20.354301477579824 27.500369777485034 32.45604383994428 19.689284904990867 36-37 20.31432907317969 27.204800800036153 32.423185304292176 20.05768482249198 38-39 19.976300934407806 27.667201571775667 32.43186518642245 19.92463230739408 40-41 20.005900629036194 27.593599638677528 32.32473856935243 20.07576116293385 42-43 19.77572648775408 27.687387092613385 31.7820401922969 20.75484622733563 44-45 19.908858228121165 27.765153613167094 31.57159431781411 20.754393840897627 46-47 20.34716685203028 27.927421035830324 31.124105189355124 20.601306922784275 48-49 20.29714265719686 28.107251864157938 30.680077766494946 20.91552771215025 50-51 19.96387473367092 28.468911205174162 30.584017849788577 20.98319621136634 52-53 20.198176120754187 28.742607938853176 30.084385940487397 20.974829999905243 54-55 19.873134850525652 28.27089929504784 30.34515833726917 21.510807517157335 56-57 20.180618194299004 28.541770052474448 29.93025511427274 21.347356638953805 58-59 20.364400401861854 28.01857190420301 30.095198129695888 21.52182956423925 60-61 20.198880477988997 28.108144719267404 30.4577426322896 21.235232170453997 62-63 20.082600118208394 27.885738689490164 30.495505967628972 21.536155224672466 64-65 19.86875957310774 28.06046012155888 30.340509200025373 21.730271105308013 66-67 19.827255614156087 27.99634301578715 29.92223805100822 22.25416331904854 68-69 19.811352394951122 27.83700508620891 30.01453827488859 22.337104243951377 70-71 19.982136868046137 27.76149437858174 29.872552021814897 22.383816731557225 72-73 19.85493743456948 27.473091470047038 29.91126236071865 22.760708734664835 74-75 19.536282385874873 27.93401081837666 29.706941548689986 22.82276524705848 76-77 19.596343916732266 27.489212513484357 29.8584506836934 23.055992886089975 78-79 19.55414124131579 27.48779801766063 29.840022502073133 23.118038238950447 80-81 19.74016471635948 27.29261604005897 29.686257676669992 23.280961566911557 82-83 19.689874455640386 27.06109582551338 29.717752956396566 23.531276762449664 84-85 19.559758924972723 26.93085601459453 29.58584453153414 23.92354052889861 86-87 19.608900640705613 26.77079560171739 29.661003978628088 23.95929977894891 88-89 19.743041403177326 26.47313502024823 29.459639117999494 24.32418445857496 90-91 19.727064422507016 26.290261788654632 29.5994908429742 24.383182945864156 92-93 19.94514823609004 26.19521658208773 29.515191766111613 24.34444341571061 94-95 19.66667492967933 25.894191636761853 29.454262037629757 24.984871395929055 96-97 19.77150106448132 25.49323398174342 29.557656974539942 25.177607979235322 98-99 19.861550070537287 25.48550431425728 29.27476004563963 25.378185569565805 100-101 20.18791969789405 24.633355418380447 29.79010458691122 25.388620296814285 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2563.0 1 1716.0 2 981.5 3 1046.5 4 872.0 5 874.5 6 1259.5 7 2047.0 8 2960.0 9 3197.0 10 2557.5 11 2030.5 12 1991.5 13 1973.0 14 2038.5 15 2117.0 16 2171.5 17 2287.5 18 2393.5 19 2545.5 20 2737.5 21 2946.5 22 3255.0 23 3639.0 24 4039.5 25 4638.0 26 5667.5 27 6728.0 28 7474.0 29 8449.5 30 9708.5 31 10940.0 32 12454.0 33 13997.5 34 15608.0 35 17208.0 36 18641.0 37 20099.5 38 20578.0 39 20387.5 40 20487.5 41 21044.0 42 21566.0 43 22069.0 44 22742.5 45 23240.0 46 23512.5 47 24579.0 48 27053.0 49 27338.0 50 24680.5 51 21907.5 52 19782.0 53 18594.5 54 17559.0 55 16066.0 56 14437.5 57 12713.5 58 11448.5 59 10217.5 60 8730.0 61 7363.5 62 5928.5 63 4369.5 64 3135.5 65 2439.5 66 1871.0 67 1382.0 68 1025.5 69 774.0 70 620.5 71 499.0 72 395.0 73 340.0 74 301.5 75 254.0 76 196.5 77 137.5 78 94.5 79 68.0 80 51.0 81 41.0 82 27.5 83 19.5 84 15.5 85 11.0 86 8.5 87 6.5 88 5.5 89 5.0 90 4.0 91 3.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2803825166762983 2 0.16901359368783594 3 0.1928265055132699 4 0.1645123725501015 5 0.23203068961611842 6 0.19776332869659158 7 0.23812911825433933 8 0.2435015434832482 9 0.15405792345600855 10-11 0.20502336278971164 12-13 0.21141219279165735 14-15 0.17569282505350645 16-17 0.2847385371321704 18-19 0.23101428484308162 20-21 0.2005221416519772 22-23 0.244517948256285 24-25 0.23428130018498566 26-27 0.3383175887393967 28-29 0.31138286225392114 30-31 0.2859727429280008 32-33 0.19551271812772433 34-35 0.3598798899959634 36-37 0.3968334635299447 38-39 0.37737657216038284 40-41 0.3389709918077775 42-43 0.3739643561366164 44-45 0.3815873919343925 46-47 0.3866694157995766 48-49 0.40692491091938165 50-51 0.4001004788718488 52-53 0.39879367273508715 54-55 0.39175143966476067 56-57 0.3714959445449556 58-59 0.34797343408324644 60-61 0.313488272140926 62-63 0.38122439022973653 64-65 0.4154917511492634 66-67 0.4197751712642042 68-69 0.42485719512938835 70-71 0.4257283992205627 72-73 0.4152739501264698 74-75 0.41120833103432247 76-77 0.39523625602945833 78-79 0.36924533397608833 80-81 0.3794819820473877 82-83 0.3905172338689302 84-85 0.39080763523265505 86-87 0.38645161477678297 88-89 0.39436505193828386 90-91 0.41563695183112576 92-93 0.4143301456943642 94-95 0.4232599876289019 96-97 0.4242763924019387 98-99 0.42028337365072266 100-101 0.3945828529610775 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 688702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.78182291213777 #Duplication Level Percentage of deduplicated Percentage of total 1 83.63125162176259 54.17784932481459 2 10.259577137012624 13.292682184867385 3 2.682149378023891 5.2126357809313255 4 1.0963919617229134 2.8410507962650042 5 0.5590424103414648 1.8107893213557718 6 0.35643410926293806 1.3854270807670332 7 0.2322104255757892 1.0530110267602044 8 0.16702806518570462 0.8656306032173856 9 0.1316928669048463 0.7678173584359335 >10 0.8047903837988977 9.641604979984916 >50 0.055871513705064685 2.459048376421482 >100 0.02041877647653967 2.475170547806207 >500 0.001121910795295212 0.49654584160225346 >1k 0.0015706751134132969 1.7912571446324854 >5k 4.487643181180848E-4 1.7294796321380597 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6819 0.9901234496197194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5079 0.7374742631791399 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3577 0.519382839021812 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2196 0.31886069736983486 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1716 0.24916437007588185 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1631 0.2368223121175777 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1091 0.1584139439118806 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1090 0.15826874323001822 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1022 0.14839509686337488 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 969 0.14069946072466757 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.4520068186240202E-4 0.0 20-21 0.0 0.0 0.0 4.3560204558720604E-4 0.0 22-23 0.0 0.0 0.0 0.0018876088642112263 0.0 24-25 0.0 0.0 0.0 0.005372425228908875 0.0 26-27 0.0 0.0 0.0 0.012124256935510568 0.0 28-29 0.0 0.0 0.0 0.03252495273717806 0.0 30-31 0.0 0.0 0.0 0.08211098559318834 0.0 32-33 0.0 0.0 0.0 0.17409561755302003 0.0 34-35 0.0 0.0 0.0 0.31283486907254515 0.0 36-37 0.0 0.0 0.0 0.5217786502725417 0.0 38-39 0.0 0.0 0.0 0.8214728576365394 0.0 40-41 0.0 0.0 0.0 1.1552166248972706 0.0 42-43 0.0 0.0 0.0 1.4806113529509135 0.0 44-45 0.0 0.0 0.0 1.8673533690914212 0.0 46-47 0.0 0.0 0.0 2.298163792177168 0.0 48-49 0.0 7.260034093120101E-5 0.0 2.734419240832755 0.0 50-51 0.0 1.4520068186240202E-4 0.0 3.213291089614957 0.0 52-53 0.0 1.4520068186240202E-4 0.0 3.6650104108888897 0.0 54-55 0.0 1.4520068186240202E-4 0.0 4.143301456943642 0.0 56-57 0.0 1.4520068186240202E-4 0.0 4.714666140072193 0.0 58-59 0.0 1.4520068186240202E-4 0.0 5.3186283762788555 0.0 60-61 0.0 1.4520068186240202E-4 0.0 5.971015039886627 0.0 62-63 0.0 1.4520068186240202E-4 0.0 6.624853710313023 0.0 64-65 0.0 1.4520068186240202E-4 0.0 7.292486445516348 0.0 66-67 0.0 1.4520068186240202E-4 0.0 8.036857741083953 0.0 68-69 0.0 1.4520068186240202E-4 0.0 8.845698139398461 0.0 70-71 0.0 1.4520068186240202E-4 0.0 9.716321427845426 0.0 72-73 0.0 1.4520068186240202E-4 0.0 10.60037577936466 0.0 74-75 0.0 1.4520068186240202E-4 0.0 11.500547406570622 0.0 76-77 0.0 1.4520068186240202E-4 0.0 12.400210831390064 0.0 78-79 0.0 1.4520068186240202E-4 0.0 13.404346146809505 0.0 80-81 0.0 1.4520068186240202E-4 0.0 14.497344279528736 0.0 82-83 0.0 1.4520068186240202E-4 0.0 15.59223002111218 0.0 84-85 0.0 1.4520068186240202E-4 0.0 16.68653495996817 0.0 86-87 0.0 1.4520068186240202E-4 0.0 17.761963810182053 0.0 88-89 0.0 2.9040136372480405E-4 0.0 18.888721101434292 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5905 0.0 24.949856 1 GTATCAA 7880 0.0 24.305532 1 TAATACC 225 2.8521754E-9 23.190691 4 GAGTACT 4445 0.0 22.421898 12-13 GTACATG 3485 0.0 22.228548 1 GTACACG 150 3.0183997E-5 22.178545 1 TACATGG 3690 0.0 20.953896 2 AGTACTT 4515 0.0 20.865444 12-13 ATCAACG 9125 0.0 20.793646 3 TATCAAC 9155 0.0 20.77732 2 TCAACGC 9165 0.0 20.702892 4 GTATAGT 115 0.003710235 20.66324 1 CAACGCA 9220 0.0 20.579393 5 AACGCAG 9365 0.0 20.362062 6 ACATGGG 3650 0.0 20.013884 3 GTACTTT 5060 0.0 19.64981 14-15 AGAGTAC 7210 0.0 18.82384 10-11 CATGGGG 1145 0.0 18.642775 4 CGCAGAG 10120 0.0 18.514845 8 ACGCAGA 10230 0.0 18.501236 7 >>END_MODULE