FastQCFastQC Report
Thu 26 May 2016
SRR938776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688702
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62580.9086658670949118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.7522847327291049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.5487133767580173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23660.3435448132864432No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18060.26223243144349806No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT16730.24292074075579856No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15390.2234638493862367No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10690.15521952891090776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.1492663009545493No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.14839509686337488No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT9410.1366338416325203RNA PCR Primer, Index 17 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.12617939253842736No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG856.3340785E-427.9940811
GGTCCGA700.00843996227.1093588
GTGTAGG2059.349606E-1025.5360661
GGTATCA64450.023.7765121
CATCCCG1000.001657875323.737925
GTATCAA83800.021.8641151
AGTACTT47800.020.94473612-13
GAGTACT47150.020.73031412-13
TAATACC2801.5825208E-920.346794
GTACATG41100.019.9159851
TACATGG41600.019.4011862
GTACAGG3254.7475623E-1019.0359761
GTACTTT51250.018.84043314-15
CATGGGA27000.018.81454
GTGCTAG2051.5622109E-518.5716841
CGGTATA1152.077135E-618.56940834-35
ATGGGAT11000.018.5587395
ACATGGG40600.018.475823
ATCAACG99000.018.4148713
TATCAAC98800.018.4040972
Produced by FastQC (version 0.11.5)