##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938776_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688702 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.994525934293787 31.0 31.0 34.0 30.0 34.0 2 32.19097664882634 33.0 31.0 34.0 30.0 34.0 3 32.25321692110666 34.0 31.0 34.0 30.0 34.0 4 35.903684031700216 37.0 35.0 37.0 35.0 37.0 5 35.81487784266635 37.0 35.0 37.0 35.0 37.0 6 35.85492564273082 37.0 35.0 37.0 35.0 37.0 7 35.771599327430444 37.0 35.0 37.0 33.0 37.0 8 35.76854866110451 37.0 35.0 37.0 33.0 37.0 9 37.385236285069595 39.0 37.0 39.0 34.0 39.0 10-11 37.32768091278957 39.0 37.0 39.0 34.0 39.0 12-13 37.25729270424654 39.0 37.0 39.0 33.0 39.0 14-15 38.41176444964586 40.0 38.0 41.0 33.0 41.0 16-17 38.36138416906006 40.0 38.0 41.0 33.0 41.0 18-19 38.51490775400681 40.0 38.0 41.0 34.0 41.0 20-21 38.52744147686518 40.0 38.0 41.0 34.0 41.0 22-23 38.42704319139483 40.0 38.0 41.0 34.0 41.0 24-25 38.31871259267433 40.0 38.0 41.0 33.0 41.0 26-27 38.12100444023685 40.0 37.0 41.0 33.0 41.0 28-29 37.92144207509199 40.0 37.0 41.0 33.0 41.0 30-31 37.6199009150547 40.0 36.5 41.0 33.0 41.0 32-33 37.344488617718554 39.5 36.0 41.0 32.0 41.0 34-35 37.20002773333023 40.0 35.5 41.0 31.5 41.0 36-37 37.00199941338924 40.0 35.0 41.0 31.0 41.0 38-39 36.818894093526666 39.5 35.0 41.0 31.0 41.0 40-41 36.59489154961072 39.0 35.0 41.0 30.5 41.0 42-43 36.306925201320745 39.0 35.0 41.0 30.0 41.0 44-45 35.94120533990028 39.0 35.0 41.0 29.5 41.0 46-47 35.44576318930393 39.0 35.0 41.0 26.5 41.0 48-49 34.9831051746619 39.0 35.0 41.0 24.5 41.0 50-51 34.67337542217098 39.0 35.0 40.5 23.5 41.0 52-53 34.42780839898824 38.0 34.0 40.0 23.0 41.0 54-55 34.14630420704455 38.0 34.0 40.0 21.5 41.0 56-57 33.84475506096977 38.0 34.0 40.0 20.0 41.0 58-59 33.513850693042855 38.0 33.0 40.0 18.0 41.0 60-61 33.12886633115629 37.0 33.0 40.0 14.0 41.0 62-63 32.753119636649814 37.0 32.5 40.0 9.5 41.0 64-65 32.33513406378957 36.0 32.0 39.0 7.0 41.0 66-67 31.89932002520684 36.0 31.5 39.0 2.0 40.0 68-69 31.567599629447862 35.0 31.0 39.0 2.0 40.0 70-71 31.15112051366193 35.0 31.0 38.0 2.0 40.0 72-73 30.67242943972865 35.0 30.5 37.0 2.0 39.5 74-75 30.201243063037424 35.0 30.0 37.0 2.0 39.0 76-77 28.263563921696175 32.5 27.0 35.0 2.0 37.5 78-79 29.13967434391072 34.0 29.0 36.0 2.0 38.0 80-81 29.14827022427697 34.0 29.0 35.5 2.0 37.0 82-83 28.80684534094572 34.0 29.0 35.0 2.0 37.0 84-85 28.41487174423771 34.0 29.0 35.0 2.0 36.5 86-87 27.855308246527528 34.0 27.5 35.0 2.0 36.0 88-89 27.269864905285594 33.5 26.5 35.0 2.0 35.5 90-91 26.76057278764981 33.0 25.5 35.0 2.0 35.0 92-93 26.465216450656452 33.0 25.0 35.0 2.0 35.0 94-95 26.150769128011824 33.0 24.5 35.0 2.0 35.0 96-97 25.595905050370114 33.0 21.5 35.0 2.0 35.0 98-99 24.86153880778624 32.5 10.0 34.5 2.0 35.0 100-101 21.728148749386527 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.011362214384785574 1101 2 0.13073606666811344 1101 3 0.018121184463204543 1101 4 0.1183555637442808 1101 5 -0.29454146553764105 1101 6 -0.11731299492085867 1101 7 0.0871649976542912 1101 8 0.18818038716293728 1101 9 0.2867781870970987 1101 10-11 0.19973183924332005 1101 12-13 0.2993373577883176 1101 14-15 0.21721065633376924 1101 16-17 0.044490330375374754 1101 18-19 0.15724832970705904 1101 20-21 0.25003497969417054 1101 22-23 0.17832226344074797 1101 24-25 0.083608347821162 1101 26-27 -0.09888983360293224 1101 28-29 0.03751477127007519 1101 30-31 0.2948289865568938 1101 32-33 0.3136501172885531 1101 34-35 0.27324193979000455 1101 36-37 0.26794955345187077 1101 38-39 0.12731701555316022 1101 40-41 0.28209323922109064 1101 42-43 0.3790433215902098 1101 44-45 0.3261726737171955 1101 46-47 0.4158717669780643 1101 48-49 0.4362887768852488 1101 50-51 0.19252536533713993 1101 52-53 0.3295917270449351 1101 54-55 0.3510648353067225 1101 56-57 0.38941251499218765 1101 58-59 0.30683444677518423 1101 60-61 0.48232206207234896 1101 62-63 0.2722783208656807 1101 64-65 0.4218279151597599 1101 66-67 0.4575327094650916 1101 68-69 0.39053029208836065 1101 70-71 0.4295898754772587 1101 72-73 0.4012180626963051 1101 74-75 0.1862396382504663 1101 76-77 0.18070602614131204 1101 78-79 -0.13382083552195212 1101 80-81 0.12967336528715023 1101 82-83 0.4056448266369692 1101 84-85 0.37508793979952415 1101 86-87 0.25609161608002395 1101 88-89 0.30607113518853524 1101 90-91 0.0729078913680219 1101 92-93 0.03522352115114202 1101 94-95 0.3446445592905043 1101 96-97 0.44912345364783235 1101 98-99 0.14395342023038893 1101 100-101 0.8649598481746352 1102 1 0.256246667534338 1102 2 0.19670172014119913 1102 3 0.15124461492675323 1102 4 0.060204447047645715 1102 5 -0.09402851818493474 1102 6 -0.07060403572268115 1102 7 0.010911429639747894 1102 8 0.07511359927847394 1102 9 0.0842976788736749 1102 10-11 0.03220728929780137 1102 12-13 0.022706766784729382 1102 14-15 0.22576943470797772 1102 16-17 0.36502783324426247 1102 18-19 0.3785811015167937 1102 20-21 0.2816897585496818 1102 22-23 0.1774109017237322 1102 24-25 0.14327914346890225 1102 26-27 0.08671822738031665 1102 28-29 0.2061891076360851 1102 30-31 0.14700901280588852 1102 32-33 0.22805453460755132 1102 34-35 0.024585028481766358 1102 36-37 0.0877818094174998 1102 38-39 -0.046178179186355806 1102 40-41 0.1808240176220437 1102 42-43 0.4035787833653828 1102 44-45 0.2177874774401829 1102 46-47 0.14191509788258116 1102 48-49 0.1468623554172197 1102 50-51 0.2172468875117488 1102 52-53 0.30258065923650435 1102 54-55 -0.00412669549128708 1102 56-57 -0.17144483350227802 1102 58-59 0.12487740333063613 1102 60-61 0.0924453075896352 1102 62-63 -0.10638467361489745 1102 64-65 0.33113628297554953 1102 66-67 0.20814826857102275 1102 68-69 0.18184071436747828 1102 70-71 -0.04454884313479823 1102 72-73 0.11846530465727056 1102 74-75 -0.11943531534585006 1102 76-77 -0.2702976972949429 1102 78-79 -0.19956143390111336 1102 80-81 -0.17310929611189252 1102 82-83 -0.23005225366313553 1102 84-85 -0.21680472897508452 1102 86-87 -0.3211546454429097 1102 88-89 -0.466573083903711 1102 90-91 -0.34979793934152426 1102 92-93 -0.4911123813754905 1102 94-95 -0.4279461813635841 1102 96-97 -0.5612090262587621 1102 98-99 -0.33262636625694597 1102 100-101 0.49038126940869375 1103 1 0.28097604780540664 1103 2 0.22201587293576353 1103 3 0.17965806209580393 1103 4 0.006970654992755954 1103 5 0.03895525112880449 1103 6 0.1688473177008376 1103 7 0.07927783510801589 1103 8 0.07441187225028756 1103 9 0.13506892885742872 1103 10-11 0.08394341394531324 1103 12-13 0.16040445238503054 1103 14-15 0.06641567332115272 1103 16-17 0.0467653873357321 1103 18-19 -0.017173449962683662 1103 20-21 0.1710313046882348 1103 22-23 0.2826537122704309 1103 24-25 0.27890738408331117 1103 26-27 0.10019723404672476 1103 28-29 -0.07411185528933828 1103 30-31 0.12258826247776256 1103 32-33 0.3665449118508022 1103 34-35 0.1483846204404955 1103 36-37 0.3185200522410412 1103 38-39 0.10942654057765822 1103 40-41 0.13139449570606843 1103 42-43 0.22849788983295838 1103 44-45 0.1928664217308551 1103 46-47 0.2296472200870383 1103 48-49 0.14697606118567563 1103 50-51 0.2045508262795721 1103 52-53 0.18226696126501452 1103 54-55 0.13764815409222564 1103 56-57 0.0501839528998147 1103 58-59 0.07709174195291268 1103 60-61 -0.07956553019447199 1103 62-63 -0.16446882887849767 1103 64-65 0.014348763320690239 1103 66-67 0.07249078933044473 1103 68-69 0.06820966684496454 1103 70-71 0.05654208249513104 1103 72-73 0.2100114432093747 1103 74-75 0.3273211463905348 1103 76-77 0.22847103166948912 1103 78-79 -0.02840501374855009 1103 80-81 0.17001897698986923 1103 82-83 0.2868606672106466 1103 84-85 0.14798286864943933 1103 86-87 0.26614864357766166 1103 88-89 0.31741844339217096 1103 90-91 0.42729976964967875 1103 92-93 0.24420982828494786 1103 94-95 0.3512112264047964 1103 96-97 0.374258077038057 1103 98-99 0.6063786108069742 1103 100-101 1.2776930051434903 1104 1 0.16125142324118613 1104 2 0.059036521903315986 1104 3 0.030941918357541454 1104 4 -0.063788094001616 1104 5 -0.44997023157433347 1104 6 -0.23253793974083692 1104 7 -0.20220681225052317 1104 8 -0.13135291053330178 1104 9 -0.01784572654616312 1104 10-11 -0.006511047706602824 1104 12-13 -0.147159572822801 1104 14-15 -0.09953238580997947 1104 16-17 0.14911144190799774 1104 18-19 0.12000935709014726 1104 20-21 -0.020815956641875744 1104 22-23 -0.18795670607229198 1104 24-25 -0.07072975053723951 1104 26-27 -0.09424371862154857 1104 28-29 -0.14678758940036118 1104 30-31 -0.15587111000814957 1104 32-33 -0.1100699647917267 1104 34-35 -0.21927426934228578 1104 36-37 0.16679120402436354 1104 38-39 -0.07269333070149742 1104 40-41 -0.01671198391313311 1104 42-43 -0.48434192982377766 1104 44-45 -0.3946088564938748 1104 46-47 -0.3413210354774563 1104 48-49 -0.27419499673870007 1104 50-51 -0.27589592553056974 1104 52-53 -0.32470541738878467 1104 54-55 -0.19060254984984937 1104 56-57 -0.36087631795028585 1104 58-59 -0.15581880712191065 1104 60-61 -0.027186792597262865 1104 62-63 -0.05914778101043794 1104 64-65 0.005134664366551078 1104 66-67 -0.22795839275590524 1104 68-69 -0.5224857273625965 1104 70-71 -0.5511359142868493 1104 72-73 -0.20921958387563677 1104 74-75 -0.26300551625360313 1104 76-77 -0.5448240038628249 1104 78-79 -0.6197854944404249 1104 80-81 -0.4899853807467913 1104 82-83 -0.5668136590162227 1104 84-85 -0.4346067163599443 1104 86-87 -0.40917902469801604 1104 88-89 -0.44884764671241584 1104 90-91 -0.4180089592320577 1104 92-93 -0.5243105525141907 1104 94-95 -0.5655790491382469 1104 96-97 -0.33520240164255455 1104 98-99 -0.31361292512758965 1104 100-101 0.6755899781925692 1105 1 0.1657183519347747 1105 2 0.14188746256901652 1105 3 0.10751901347660464 1105 4 0.06065071339214967 1105 5 -0.19563486451736622 1105 6 0.013583047629246892 1105 7 0.06323934614968607 1105 8 0.06872561531856292 1105 9 0.20044380772564097 1105 10-11 0.11890727718486005 1105 12-13 0.13506069424732203 1105 14-15 0.18810349269284643 1105 16-17 0.042461206741570834 1105 18-19 0.1731859539195142 1105 20-21 0.12503964623932262 1105 22-23 0.08091005488021352 1105 24-25 0.10224707816951195 1105 26-27 0.21460647378734166 1105 28-29 0.13741946394788584 1105 30-31 0.06503732309275279 1105 32-33 0.09300158829339011 1105 34-35 0.13946142332972755 1105 36-37 0.1551212757254561 1105 38-39 0.30572727185079884 1105 40-41 0.16014488524881898 1105 42-43 0.11392536230882655 1105 44-45 0.28506425395212176 1105 46-47 0.2445085428675 1105 48-49 0.4754822661214533 1105 50-51 0.32196434778873595 1105 52-53 0.3646775585354405 1105 54-55 0.01819193442853617 1105 56-57 0.22646005226415866 1105 58-59 0.08217927701176109 1105 60-61 0.20905576594766728 1105 62-63 0.28281483081694603 1105 64-65 0.31537139308645834 1105 66-67 0.241798876024518 1105 68-69 0.15189305503445283 1105 70-71 0.2676091521517776 1105 72-73 0.09739303288531787 1105 74-75 0.27226328061522764 1105 76-77 0.3873713898353728 1105 78-79 0.09110883689755767 1105 80-81 0.011449463352672495 1105 82-83 0.24526390479116955 1105 84-85 0.24664924502671326 1105 86-87 0.38342022211161364 1105 88-89 0.007395775205750965 1105 90-91 0.18844496130471455 1105 92-93 0.3328217327114338 1105 94-95 0.6624121280094961 1105 96-97 0.7784113498375156 1105 98-99 0.5776709108808262 1105 100-101 1.1276347338168264 1106 1 0.17514230995643842 1106 2 -0.07563295770287226 1106 3 0.05751300901090417 1106 4 0.09244792121383227 1106 5 0.037293625349335 1106 6 -0.11909936091459628 1106 7 0.08612348438673934 1106 8 -0.05602886309691257 1106 9 -0.15512436738493562 1106 10-11 -0.0363875420807247 1106 12-13 0.015547401988605714 1106 14-15 0.006948762700439204 1106 16-17 -0.01426979930156591 1106 18-19 -0.04938534147780871 1106 20-21 0.1182077855266428 1106 22-23 0.16961026106746857 1106 24-25 0.05951054801793987 1106 26-27 -0.053067518327473806 1106 28-29 0.17235018869707375 1106 30-31 0.09085245475338866 1106 32-33 0.10433476286458188 1106 34-35 0.03040607843932719 1106 36-37 0.08861825619160157 1106 38-39 0.32302013304993693 1106 40-41 0.2703207245001096 1106 42-43 0.25667084827365017 1106 44-45 0.3358813701395462 1106 46-47 0.3060166611086643 1106 48-49 0.1408554283987442 1106 50-51 0.06967908885378193 1106 52-53 0.05114166098994133 1106 54-55 0.1256433745843637 1106 56-57 0.0962862891332108 1106 58-59 0.1635367340493019 1106 60-61 0.06042488589653061 1106 62-63 -0.02999849543314781 1106 64-65 0.24649406533828966 1106 66-67 0.25914364023220315 1106 68-69 -0.08339345974976098 1106 70-71 -0.1590305249504489 1106 72-73 -0.049161726824106466 1106 74-75 -0.047717749344002414 1106 76-77 0.09024803775589518 1106 78-79 -0.20651013802820017 1106 80-81 -0.3586362885590866 1106 82-83 0.003183184418119822 1106 84-85 0.23500974093451887 1106 86-87 0.1845889557392688 1106 88-89 0.4019746586193804 1106 90-91 -0.01044838513667301 1106 92-93 0.24308651947148618 1106 94-95 0.3755879611545545 1106 96-97 0.6758275977181398 1106 98-99 0.8777059199689425 1106 100-101 1.7020921438363459 1107 1 0.07549323308859712 1107 2 0.09394484348300836 1107 3 0.02599737418520931 1107 4 0.09527276315955646 1107 5 0.08765278099137674 1107 6 0.050813691878602185 1107 7 0.02225706132301042 1107 8 -0.025816543691398408 1107 9 0.14455089481554495 1107 10-11 -0.062366566042285854 1107 12-13 -0.11413049858251867 1107 14-15 -0.2527581599476889 1107 16-17 0.12990525361463057 1107 18-19 0.066876543588414 1107 20-21 0.004416645294263333 1107 22-23 0.09023200401874476 1107 24-25 -0.07264305673034244 1107 26-27 0.02964936863322265 1107 28-29 0.2551940348463333 1107 30-31 0.10356920285246929 1107 32-33 0.20732048590707564 1107 34-35 0.18292473052949276 1107 36-37 0.2166794108457495 1107 38-39 6.013224591612243E-4 1107 40-41 -0.13047356392339537 1107 42-43 0.1505161048601522 1107 44-45 0.06809369351253736 1107 46-47 -0.19447728948437515 1107 48-49 0.03771398197651621 1107 50-51 0.07550830097282812 1107 52-53 0.08797569557442131 1107 54-55 0.1364686291474584 1107 56-57 0.24515089707728066 1107 58-59 0.5210518253309715 1107 60-61 0.37568637223156287 1107 62-63 0.3875831700730288 1107 64-65 0.29065404185084986 1107 66-67 0.2075942421972954 1107 68-69 -0.0881772423004108 1107 70-71 -0.10877551551777387 1107 72-73 -0.04369355489756188 1107 74-75 -0.013288004393110953 1107 76-77 -0.12649212637853324 1107 78-79 0.010719017355960148 1107 80-81 0.37087418458209953 1107 82-83 0.10371601626265559 1107 84-85 -0.011881246748455254 1107 86-87 0.12766263769459485 1107 88-89 0.1233210608141917 1107 90-91 0.40858339235147767 1107 92-93 0.48920880894240426 1107 94-95 0.586616945540559 1107 96-97 0.879831176380268 1107 98-99 0.7105939042736118 1107 100-101 1.380448974538254 1108 1 0.09583208414469979 1108 2 0.03142915994012441 1108 3 0.11477487987454538 1108 4 0.09410893611551074 1108 5 -0.01287981049235043 1108 6 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10 1427.0 11 2179.0 12 3206.0 13 4285.0 14 4808.0 15 6207.0 16 8043.0 17 10889.0 18 10226.0 19 6303.0 20 5516.0 21 5514.0 22 5866.0 23 6248.0 24 7092.0 25 7826.0 26 9054.0 27 10377.0 28 12480.0 29 14886.0 30 17731.0 31 21396.0 32 25546.0 33 32111.0 34 42315.0 35 59135.0 36 83979.0 37 131619.0 38 118721.0 39 11987.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49680171506665 21.09150660687802 15.45743115500657 20.954260523048752 2 15.58874520474748 23.105784504764035 39.137972591919294 22.167497698569193 3 18.5399490636008 27.5320820906575 32.278401979375694 21.649566866366005 4 12.46039651402203 19.171281628338527 37.908703619272195 30.45961823836725 5 12.081634550163185 39.04119636940926 34.42888204937522 14.44828703105233 6 27.788063199438923 38.15184767314721 18.409065498049156 15.65102362936471 7 24.658270195236838 35.79022567089975 22.2864170570145 17.26508707684891 8 24.977566494652258 34.134937897668365 21.397062880607287 19.490432727072086 9 25.82103725559095 17.189727922962327 22.327218448617835 34.66201637282889 10-11 23.502254966589323 27.777326042323097 28.16152704653101 20.55889194455657 12-13 25.464061379232238 26.211336688437086 28.43646453763747 19.888137394693207 14-15 22.330848465664392 27.119639553827348 28.43283452059091 22.11667745991735 16-17 19.796494004813574 30.165933982161462 29.03833797387021 20.999234039154757 18-19 19.50081457582525 28.245961243033996 32.564084320939976 19.689139860200783 20-21 20.97844931479798 27.13401442133172 32.34773530496499 19.539800958905303 22-23 20.554245522736974 27.422165174487663 32.161951032522055 19.86163827025332 24-25 20.31314092721136 27.726388649911897 32.48230904770513 19.47816137517161 26-27 20.19621210306181 27.627674720321888 32.75883974511158 19.417273431504725 28-29 19.589459592102244 28.129728097203145 32.6014008961786 19.679411414516 30-31 20.12590351124289 27.751988523338106 32.363126577242404 19.7589813881766 32-33 19.57806133857605 28.14453856675311 32.316589758705504 19.960810335965338 34-35 20.348901795042885 27.87068546779806 32.20389561262564 19.576517124533417 36-37 20.216446067112223 27.65510951555234 32.094330635018835 20.034113782316602 38-39 19.90540288365818 27.83847622256317 32.27701823034848 19.97910266343017 40-41 20.03835098044768 27.740348905341623 32.17532540610871 20.04597470810199 42-43 19.863993883645502 27.84233687724671 31.705209719659827 20.58845951944796 44-45 19.809184441729087 27.97398774894707 31.390909071108236 20.825918738215606 46-47 20.420807623644276 28.030149579025395 30.82999189051248 20.719050906817852 48-49 20.270817724033385 28.173334378793424 30.479953302782665 21.075894594390526 50-51 19.912966711291677 28.58456923313712 30.42193866142395 21.08052539414725 52-53 20.128734924539206 28.826909171165465 30.024088793120974 21.020267111174356 54-55 19.75600477419842 28.492512000836356 30.071279014726255 21.68020421023897 56-57 20.287776992070487 28.79490571270508 29.49049866921836 21.42681862600607 58-59 20.25291275105417 28.475699002172224 29.827311324590237 21.44407692218337 60-61 20.10624573009635 28.810496503125876 29.79512448697629 21.288133279801478 62-63 19.77819015010934 28.767616575044503 29.818294811368702 21.63589846347746 64-65 19.583266781861212 28.853112430846974 29.57622442608431 21.98739636120751 66-67 19.648914419804143 28.73317031237294 29.272620610341182 22.34529465748173 68-69 19.708901461663725 28.571086312429987 29.28641799095835 22.433594234947943 70-71 19.876535770576947 28.582557174625002 29.045457284469396 22.495449770328655 72-73 19.818658868422045 28.375843252959914 29.015089254859138 22.7904086237589 74-75 19.5532429742274 28.915785109986764 28.551691492551505 22.97928042323433 76-77 19.583870892755442 28.532927693899136 28.85495484500854 23.028246568336876 78-79 19.453203098588478 28.686516923367734 28.74540609902833 23.11487387901546 80-81 19.564986960789437 28.712399737473355 28.384677039954 23.337936261783206 82-83 19.506169254280724 28.555715680687094 28.3954856813042 23.542629383727977 84-85 19.41579957659481 28.308179735212036 28.284366823386602 23.99165386480655 86-87 19.381430411284672 28.389457250756607 28.206447636574012 24.02266470138471 88-89 19.41990991240431 28.13801967587701 28.10574144481151 24.33632896690716 90-91 19.332042163945466 28.098678968979137 28.142487369549734 24.426791497525663 92-93 19.477288270458562 28.19607171223339 27.95097118181145 24.375668835496597 94-95 19.272860857936486 27.90863642897696 27.965700545521337 24.852802167565223 96-97 19.220024364709793 27.847353205527508 27.89933512511235 25.033287304650347 98-99 19.153639745492246 27.93196476850655 27.659713490014546 25.254681995986655 100-101 19.20873765966746 27.501934813132628 28.226564076800102 25.06276345039981 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1998.0 1 1346.5 2 801.0 3 892.5 4 773.5 5 755.5 6 1098.0 7 1938.0 8 2728.5 9 2841.5 10 2315.0 11 1822.5 12 1805.0 13 1865.5 14 1959.0 15 1994.0 16 2003.5 17 2115.5 18 2235.0 19 2397.5 20 2644.0 21 2908.5 22 3201.0 23 3628.0 24 4025.5 25 4649.5 26 5759.5 27 6826.5 28 7747.0 29 8728.0 30 9924.0 31 11205.0 32 12561.0 33 13993.5 34 15576.0 35 17144.0 36 18683.0 37 20127.0 38 20535.5 39 20427.5 40 20737.5 41 21293.0 42 21935.5 43 22679.0 44 23628.0 45 24219.5 46 24302.0 47 25380.0 48 27739.0 49 28192.0 50 25764.5 51 22791.0 52 20021.0 53 18329.0 54 17113.0 55 15462.5 56 13867.0 57 12129.5 58 11050.5 59 9979.0 60 8508.0 61 7136.0 62 5616.0 63 4162.0 64 2966.0 65 2197.5 66 1587.5 67 1135.0 68 847.5 69 626.0 70 489.5 71 383.5 72 302.5 73 277.0 74 243.0 75 209.0 76 158.0 77 97.5 78 57.0 79 34.0 80 19.5 81 17.5 82 16.0 83 8.5 84 3.5 85 3.0 86 2.5 87 2.0 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23449910120777925 2 0.0 3 0.0 4 0.0 5 0.03179894932786605 6 0.0036300170465600507 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0035574167056288495 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.630017046560051E-4 26-27 0.0012342057958304171 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008058637843363312 36-37 0.01800488455093785 38-39 0.014157066481584199 40-41 0.009075042616400126 42-43 0.008203838525225713 44-45 0.0038478180693536537 46-47 3.630017046560051E-4 48-49 0.0014520068186240204 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0013068061367616183 58-59 8.712040911744122E-4 60-61 0.002250610568867231 62-63 5.808027274496081E-4 64-65 0.0017424081823488244 66-67 0.005517625910771277 68-69 7.260034093120101E-5 70-71 7.260034093120101E-5 72-73 0.0 74-75 7.986037502432111E-4 76-77 0.058951476836135226 78-79 0.14077206106559878 80-81 0.0014520068186240204 82-83 6.534030683808091E-4 84-85 0.0 86-87 0.11035251821542554 88-89 0.36024289170061946 90-91 0.4008264822811608 92-93 7.260034093120102E-4 94-95 4.356020455872061E-4 96-97 1.4520068186240202E-4 98-99 0.0 100-101 7.260034093120102E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 688702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.50254392181645 #Duplication Level Percentage of deduplicated Percentage of total 1 82.15479902358852 51.34883934359845 2 11.137982938934408 13.9230453568238 3 2.9386570261575216 5.51020619545695 4 1.2093017586956067 3.023377451504081 5 0.6463595717491981 2.019955876127036 6 0.37888136099527436 1.4208629344058838 7 0.2443620669393078 1.0691275575189956 8 0.19204966142687965 0.9602873918802818 9 0.15258524645659877 0.8583269461627278 >10 0.8540037285845119 9.901247765561974 >50 0.061297672610674536 2.6332634356601825 >100 0.02554057675636315 2.8301852364103044 >500 0.0018574964911782126 0.7685704548820985 >1k 0.0018574964911782126 2.07264411567714 >5k 4.6437412279455315E-4 1.6600599383301218 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6258 0.9086658670949118 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5181 0.7522847327291049 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3779 0.5487133767580173 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2366 0.3435448132864432 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1806 0.26223243144349806 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1673 0.24292074075579856 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1539 0.2234638493862367 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1069 0.15521952891090776 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1028 0.1492663009545493 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1022 0.14839509686337488 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 941 0.1366338416325203 RNA PCR Primer, Index 17 (95% over 21bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 869 0.12617939253842736 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.9040136372480405E-4 0.0 22-23 0.0 0.0 0.0 0.0015246071595552213 0.0 24-25 0.0 0.0 0.0 0.005082023865184071 0.0 26-27 0.0 0.0 0.0 0.011979056253648167 0.0 28-29 0.0 0.0 0.0 0.03281535410090286 0.0 30-31 0.0 0.0 0.0 0.08378079343460597 0.0 32-33 0.0 0.0 0.0 0.17903244073634172 0.0 34-35 0.0 0.0 0.0 0.32031270418845886 0.0 36-37 0.0 0.0 0.0 0.5348467116401578 0.0 38-39 0.0 0.0 0.0 0.8408571486651701 0.0 40-41 0.0 0.0 0.0 1.182078751041815 0.0 42-43 0.0 0.0 0.0 1.5127007036425044 0.0 44-45 0.0 0.0 0.0 1.903363138193297 0.0 46-47 0.0 0.0 0.0 2.340417190599127 0.0 48-49 0.0 0.0 0.0 2.7810286597105858 0.0 50-51 0.0 0.0 0.0 3.266797540881252 0.0 52-53 0.0 1.4520068186240202E-4 0.0 3.726285098634823 0.0 54-55 0.0 1.4520068186240202E-4 0.0 4.213578586965045 0.0 56-57 0.0 1.4520068186240202E-4 0.0 4.79365531100534 0.0 58-59 0.0 1.4520068186240202E-4 0.0 5.405530984373502 0.0 60-61 0.0 1.4520068186240202E-4 0.0 6.068444697416298 0.0 62-63 0.0 1.4520068186240202E-4 0.0 6.733463820346101 0.0 64-65 0.0 1.4520068186240202E-4 0.0 7.412930411121211 0.0 66-67 0.0 1.4520068186240202E-4 0.0 8.167901356464771 0.0 68-69 0.0 1.4520068186240202E-4 0.0 8.986107198759406 0.0 70-71 0.0 1.4520068186240202E-4 0.0 9.868346541755361 0.0 72-73 0.0 1.4520068186240202E-4 0.0 10.765614155324073 0.0 74-75 0.0 1.4520068186240202E-4 0.0 11.680886653443725 0.0 76-77 0.0 1.4520068186240202E-4 0.0 12.59521534713127 0.0 78-79 0.0 1.4520068186240202E-4 0.0 13.614015931418812 0.0 80-81 0.0 1.4520068186240202E-4 0.0 14.717686314254932 0.0 82-83 0.0 1.4520068186240202E-4 0.0 15.826946923342751 0.0 84-85 0.0 1.4520068186240202E-4 0.0 16.935844530725916 0.0 86-87 0.0 1.4520068186240202E-4 0.0 18.02586604946697 0.0 88-89 0.0 1.4520068186240202E-4 0.0 19.16736120992824 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACG 85 6.3340785E-4 27.994081 1 GGTCCGA 70 0.008439962 27.109358 8 GTGTAGG 205 9.349606E-10 25.536066 1 GGTATCA 6445 0.0 23.776512 1 CATCCCG 100 0.0016578753 23.73792 5 GTATCAA 8380 0.0 21.864115 1 AGTACTT 4780 0.0 20.944736 12-13 GAGTACT 4715 0.0 20.730314 12-13 TAATACC 280 1.5825208E-9 20.34679 4 GTACATG 4110 0.0 19.915985 1 TACATGG 4160 0.0 19.401186 2 GTACAGG 325 4.7475623E-10 19.035976 1 GTACTTT 5125 0.0 18.840433 14-15 CATGGGA 2700 0.0 18.8145 4 GTGCTAG 205 1.5622109E-5 18.571684 1 CGGTATA 115 2.077135E-6 18.569408 34-35 ATGGGAT 1100 0.0 18.558739 5 ACATGGG 4060 0.0 18.47582 3 ATCAACG 9900 0.0 18.414871 3 TATCAAC 9880 0.0 18.404097 2 >>END_MODULE