FastQCFastQC Report
Thu 26 May 2016
SRR938775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191106
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76470.64200835190151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59520.4997036367879936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39680.33313575785866245No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33920.28477734139530825No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.1603551657031364No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16690.1401218699259344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.10880643704254701No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.10158625680669899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60700.031.307351
GTATCAA87100.029.4897941
GTACATG56600.025.7884311
TATCAAC102700.024.8174062
ATCAACG103600.024.647543
TCAACGC103950.024.564554
CAACGCA104950.024.3756315
TACATGG58450.024.3153922
AACGCAG107100.023.8200346
GAGTACT56350.023.22731412-13
ACATGGG59950.023.1538373
TAAGGTA2052.7454007E-823.1095094
ACGCAGA117250.021.6799987
CGCAGAG116300.021.490778
CATGGGA34350.021.2392184
CCTATAA5600.021.1564431
GTACTTT63200.020.78513314-15
TATAAGC6600.020.0982693
AGTACTT59300.019.95644212-13
GCAGAGT119050.019.8810069
Produced by FastQC (version 0.11.5)