FastQCFastQC Report
Thu 26 May 2016
SRR938775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191106
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70240.5897040229836806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61210.5138921305072764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42510.3568951881696507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37370.3137420179228381No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT22390.187976552884462No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17820.14960885093350215No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.1494409397652266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.1125004827446088No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.10108252330187238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66000.028.4452671
GTATCAA90100.025.2790571
GTACATG57300.021.7873481
TGGACCG1100.002894564321.5688255
GAGTACT55800.021.13292312-13
TACATGG58150.020.9716472
ATCAACG108250.020.8654693
GTATAGT1604.8701964E-520.8466281
TATCAAC108450.020.8269922
CATGGGG23600.020.7097264
TCAACGC108700.020.6917824
ACATGGG57150.020.591343
CAACGCA109850.020.4751665
GGGTATA1656.171979E-520.2149121
AGTACTT58350.020.1280512-13
AACGCAG112450.019.9595536
TTATAGG5200.019.1630693
AGAGTAC95250.018.80699210-11
ATGGGGA13700.018.3571155
GTACTTT63850.018.31991214-15
Produced by FastQC (version 0.11.5)