FastQCFastQC Report
Thu 26 May 2016
SRR938774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191610
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77720.6522268191774154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58720.4927786775874657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39430.3308968538364062No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33660.2824749708377741No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.16372806539052207No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16730.1403982846736768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.10859257643020787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58750.031.2280081
GTATCAA84100.028.653441
GTACATG51300.023.9038141
ATCAACG100250.023.8549963
TATCAAC100800.023.7719122
TCAACGC100800.023.6306924
CAACGCA101650.023.3864125
AACGCAG103450.023.0243916
GAGTACT53850.022.34596412-13
TACATGG53850.021.8524042
ACGCAGA112600.020.9857377
AGTACTT56650.020.94821212-13
CGCAGAG112900.020.8459158
ACATGGG53200.020.781533
AGAGTAC87700.020.02583910-11
GAACGCG600.00476575619.791178-79
ACGATAC1456.190238E-419.6343483
GTACTTT61750.019.56441314-15
GCAGAGT116550.019.540879
GCGGTAT1507.688898E-419.011871
Produced by FastQC (version 0.11.5)