##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938774_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1191610 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.502569632681833 33.0 31.0 34.0 30.0 34.0 2 31.55789394180982 33.0 31.0 34.0 28.0 34.0 3 31.55744413021039 33.0 31.0 34.0 28.0 34.0 4 35.13637096029741 37.0 35.0 37.0 33.0 37.0 5 35.16566494071047 37.0 35.0 37.0 33.0 37.0 6 35.235842263827934 37.0 35.0 37.0 33.0 37.0 7 35.13588422386519 37.0 35.0 37.0 32.0 37.0 8 35.112180998816726 37.0 35.0 37.0 32.0 37.0 9 36.67528134204983 39.0 37.0 39.0 32.0 39.0 10-11 36.56129018722568 39.0 37.0 39.0 32.0 39.0 12-13 36.45369164407818 39.0 37.0 39.0 32.0 39.0 14-15 37.583492082140964 40.0 37.0 41.0 32.0 41.0 16-17 37.47585115935583 40.0 37.0 41.0 32.0 41.0 18-19 37.422498132778344 40.0 37.0 41.0 32.0 41.0 20-21 37.31901293208348 40.0 37.0 41.0 31.5 41.0 22-23 37.287953692902875 40.0 37.0 41.0 31.5 41.0 24-25 37.20250921022818 40.0 37.0 41.0 31.0 41.0 26-27 37.06225400928156 40.0 36.5 41.0 31.0 41.0 28-29 36.87616502043454 39.5 36.0 41.0 30.5 41.0 30-31 36.6120282642811 39.0 36.0 41.0 30.0 41.0 32-33 36.38807453781019 39.0 35.0 41.0 30.0 41.0 34-35 36.06248856588985 39.0 35.0 41.0 29.5 41.0 36-37 35.8293392972533 39.0 35.0 41.0 28.5 41.0 38-39 35.63733268435142 38.5 35.0 40.0 27.0 41.0 40-41 35.48480291370498 38.5 35.0 40.5 27.0 41.0 42-43 35.43313332382239 39.0 35.0 41.0 26.5 41.0 44-45 35.20309077634461 39.0 35.0 41.0 25.5 41.0 46-47 34.82756816408053 38.5 34.5 41.0 24.0 41.0 48-49 34.47412911942666 38.0 34.0 40.0 23.0 41.0 50-51 33.29830649289616 37.0 32.5 39.5 20.5 40.5 52-53 33.34809291630651 37.5 33.0 39.5 20.0 40.5 54-55 33.8140163308465 38.0 33.5 40.0 20.0 41.0 56-57 33.69098027039048 38.0 33.0 40.0 18.5 41.0 58-59 33.46446572284556 37.5 33.0 40.0 17.5 41.0 60-61 33.15207408464178 37.0 33.0 40.0 14.5 41.0 62-63 32.741747299871605 36.5 32.5 40.0 11.5 41.0 64-65 32.32036194728141 36.0 32.0 39.0 8.0 41.0 66-67 31.921303530517534 35.5 31.5 39.0 6.5 41.0 68-69 31.47385050477925 35.0 31.0 38.5 2.0 40.0 70-71 31.018710819815208 35.0 31.0 38.0 2.0 40.0 72-73 30.580294307701344 35.0 30.0 37.0 2.0 39.5 74-75 30.091233709015533 35.0 30.0 36.5 2.0 39.0 76-77 29.602748802040935 34.5 29.0 36.0 2.0 39.0 78-79 29.14643591443509 34.0 29.0 35.5 2.0 37.5 80-81 28.651954918135967 34.0 29.0 35.0 2.0 37.0 82-83 28.215741307978284 34.0 27.5 35.0 2.0 37.0 84-85 27.83970132845478 34.0 27.0 35.0 2.0 36.0 86-87 27.376008089895183 34.0 26.0 35.0 2.0 36.0 88-89 26.884726126836803 33.5 25.5 35.0 2.0 35.5 90-91 26.39192395162847 33.0 24.5 35.0 2.0 35.0 92-93 25.876763790166244 33.0 23.0 35.0 2.0 35.0 94-95 25.304023967573283 33.0 19.0 35.0 2.0 35.0 96-97 24.661425298545666 32.0 8.5 35.0 2.0 35.0 98-99 23.86593935935415 32.0 2.0 35.0 2.0 35.0 100-101 21.571388709393176 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.08018448833053426 1101 2 0.1383045991911267 1101 3 0.3754166904827514 1101 4 0.20841010166332552 1101 5 0.2295032634447054 1101 6 0.3952123873441096 1101 7 0.35879957730586653 1101 8 0.3827056803465112 1101 9 0.4565886512385049 1101 10-11 0.33891924405315876 1101 12-13 0.3524840191223291 1101 14-15 0.2558595659025187 1101 16-17 0.6878103303362337 1101 18-19 0.7517015572220771 1101 20-21 0.6169606061340573 1101 22-23 0.7307882402182742 1101 24-25 0.56033885183205 1101 26-27 0.6467143446365711 1101 28-29 0.7269823229584915 1101 30-31 0.5748096316930642 1101 32-33 0.5676865405021019 1101 34-35 0.4293273597275373 1101 36-37 0.30907222598849415 1101 38-39 0.44951917702098854 1101 40-41 0.6106033336862495 1101 42-43 0.6430691572327092 1101 44-45 0.7361359529592377 1101 46-47 0.6930778238671778 1101 48-49 0.8290969293517776 1101 50-51 0.8769853601534265 1101 52-53 0.7701220370653985 1101 54-55 0.770695582304171 1101 56-57 0.6144078795378718 1101 58-59 0.6211777902449498 1101 60-61 0.6995583744653189 1101 62-63 0.6835667205868035 1101 64-65 0.5207444130458967 1101 66-67 0.3946583449055261 1101 68-69 0.46184976614325635 1101 70-71 0.49133289417283166 1101 72-73 0.2335348644645272 1101 74-75 0.05824948191559898 1101 76-77 0.20474413528164703 1101 78-79 0.20084219679491966 1101 80-81 -0.076324666926606 1101 82-83 -0.16960503838611984 1101 84-85 -0.15024356014400553 1101 86-87 -0.15203814503795599 1101 88-89 -0.2556680098511528 1101 90-91 -0.36943271430122593 1101 92-93 -0.5829558825710812 1101 94-95 -0.6781356409759063 1101 96-97 -0.7407920064296647 1101 98-99 -0.6854865557487351 1101 100-101 -0.5474222950585634 1102 1 0.2151116789168519 1102 2 0.13220703821551538 1102 3 -0.01656937223501842 1102 4 0.3469118438236052 1102 5 0.33229071989070036 1102 6 0.34643189953923326 1102 7 0.28040236476230973 1102 8 0.2685941820886697 1102 9 0.27453290210958414 1102 10-11 0.32890182245036925 1102 12-13 0.3855850644184997 1102 14-15 0.3394832592823107 1102 16-17 0.5223051038554019 1102 18-19 0.4241754248178964 1102 20-21 0.40267489184834204 1102 22-23 0.2721645468384821 1102 24-25 0.42183710967177035 1102 26-27 0.48077357808605115 1102 28-29 0.614177444909707 1102 30-31 0.6701929069543908 1102 32-33 0.4858050074010478 1102 34-35 0.4689615060689931 1102 36-37 0.616563515187103 1102 38-39 0.7378467031533944 1102 40-41 0.7660911385642919 1102 42-43 0.7044803070584962 1102 44-45 0.703470447732748 1102 46-47 0.6943844440762419 1102 48-49 0.8957345600137998 1102 50-51 0.8935358828015154 1102 52-53 0.4961673332326413 1102 54-55 0.6173854777745547 1102 56-57 0.6631883673427481 1102 58-59 0.5758816229975636 1102 60-61 0.5218580260332644 1102 62-63 0.3159709017714718 1102 64-65 0.4750127057288225 1102 66-67 0.36155729960934835 1102 68-69 0.37517729227565866 1102 70-71 0.2339287129881633 1102 72-73 0.25400524773978717 1102 74-75 0.2956188198946954 1102 76-77 0.29141660914924117 1102 78-79 0.20040665672523517 1102 80-81 0.319581256418342 1102 82-83 0.3452033239832204 1102 84-85 0.15637664891522718 1102 86-87 -0.01745626350485452 1102 88-89 0.0344016765599946 1102 90-91 -0.1320633763221295 1102 92-93 -0.4152729557418091 1102 94-95 -0.8898081148435004 1102 96-97 -0.9738059437118949 1102 98-99 -1.0683262770030844 1102 100-101 -0.684181876940098 1103 1 0.06753618530477468 1103 2 0.17658373198245414 1103 3 0.26701863105573054 1103 4 0.21925021006440915 1103 5 0.3182627001462137 1103 6 0.20974490766931098 1103 7 0.22674927242781706 1103 8 0.22287154852305235 1103 9 0.4030172226670743 1103 10-11 0.24924184616310896 1103 12-13 0.3330510680963883 1103 14-15 0.32306581832218484 1103 16-17 0.27989227236101044 1103 18-19 0.39015641833319137 1103 20-21 0.39513309209998937 1103 22-23 0.316448990798996 1103 24-25 0.4429411432567534 1103 26-27 0.3254687605289419 1103 28-29 0.31493873265652184 1103 30-31 0.3065819773183094 1103 32-33 0.1943515738935062 1103 34-35 0.16478969760984086 1103 36-37 0.2788475599025446 1103 38-39 0.2758203529791814 1103 40-41 0.40122500664404726 1103 42-43 0.42380346569186855 1103 44-45 0.437913500868639 1103 46-47 0.35902010270960716 1103 48-49 0.08036574226311899 1103 50-51 0.07832736799315398 1103 52-53 -0.047826750681416286 1103 54-55 0.11138924035682152 1103 56-57 0.15486168809380985 1103 58-59 0.27958129392283837 1103 60-61 0.3227088414610719 1103 62-63 0.09336455740445615 1103 64-65 -0.0839273591182561 1103 66-67 -0.07238730211345157 1103 68-69 -0.02338270336894155 1103 70-71 -0.0318747374570556 1103 72-73 4.99755095574983E-4 1103 74-75 -0.29401623382191744 1103 76-77 -0.22138796644116 1103 78-79 -0.1659793285631892 1103 80-81 -0.2542896664426699 1103 82-83 -0.2654026816304089 1103 84-85 -0.23610544554857427 1103 86-87 -0.05219209635425415 1103 88-89 -0.030061278337409902 1103 90-91 -0.23540584389859376 1103 92-93 -0.5132740082969818 1103 94-95 -0.6269279246576716 1103 96-97 -0.6293980967316415 1103 98-99 -0.30846462146688935 1103 100-101 0.05841394187523363 1104 1 0.12253906881111831 1104 2 0.16269484309356486 1104 3 0.044318828095327945 1104 4 0.03710872601981663 1104 5 0.18408954559027535 1104 6 0.19597616487808267 1104 7 0.27554360633784825 1104 8 0.16448955602016468 1104 9 0.36971272439717495 1104 10-11 0.3219497589851059 1104 12-13 0.33062111327132726 1104 14-15 0.3686132039430987 1104 16-17 0.2143300486970361 1104 18-19 0.2352655710629108 1104 20-21 0.27691684113882076 1104 22-23 0.1614650303030345 1104 24-25 0.09581954339131471 1104 26-27 0.1989203776954227 1104 28-29 0.24700963086873884 1104 30-31 0.42299603681465925 1104 32-33 0.4364895136282243 1104 34-35 0.38132150028188505 1104 36-37 0.5330016112289613 1104 38-39 0.40695681491689584 1104 40-41 0.4960638306540446 1104 42-43 0.4292385747845131 1104 44-45 0.43024968166448474 1104 46-47 0.5895579367157566 1104 48-49 0.6395842519709234 1104 50-51 0.7774109536332929 1104 52-53 0.6961133805181277 1104 54-55 0.6703133921253723 1104 56-57 0.6881151242722083 1104 58-59 0.7915598598590137 1104 60-61 0.6885191668166968 1104 62-63 0.6465201946593524 1104 64-65 0.6546857944765136 1104 66-67 0.5693355328538736 1104 68-69 0.7650715570116837 1104 70-71 0.8365142235194902 1104 72-73 0.3940773454454387 1104 74-75 0.35702092736609004 1104 76-77 0.6672801539932891 1104 78-79 0.6180941047316963 1104 80-81 0.6217450918233816 1104 82-83 0.5689609044903143 1104 84-85 0.714526522925091 1104 86-87 0.403796886248589 1104 88-89 0.7722713581485543 1104 90-91 0.7566900447019442 1104 92-93 0.7142301367433888 1104 94-95 0.8214167624818884 1104 96-97 0.42240139144598743 1104 98-99 0.2817786143078145 1104 100-101 0.9342789937783458 1105 1 -0.05086833730829099 1105 2 0.0793615097602327 1105 3 0.12034341469615484 1105 4 0.2306963295507316 1105 5 0.319021703496297 1105 6 0.3436435676358158 1105 7 0.2345094273691899 1105 8 0.22819765823829385 1105 9 0.31989326956824726 1105 10-11 0.4743495897421184 1105 12-13 0.4614055139367039 1105 14-15 0.4642753381435156 1105 16-17 0.43730913095956225 1105 18-19 0.5255709039557459 1105 20-21 0.5499414219436574 1105 22-23 0.4157410238810684 1105 24-25 0.5056614287118038 1105 26-27 0.5392089022095732 1105 28-29 0.5152959876816467 1105 30-31 0.5161999456321027 1105 32-33 0.34286104834911413 1105 34-35 0.30969172510844345 1105 36-37 0.5436481252291756 1105 38-39 0.400179126011011 1105 40-41 0.38355636831349926 1105 42-43 0.28056339380464834 1105 44-45 0.23018867884183436 1105 46-47 0.20200019413898218 1105 48-49 0.2853860695942174 1105 50-51 0.3484082411087286 1105 52-53 0.15376061322143642 1105 54-55 0.1697823342987519 1105 56-57 0.14240792622893395 1105 58-59 0.10319589470452684 1105 60-61 0.2131659010646345 1105 62-63 0.09688476259707812 1105 64-65 0.1315554368058045 1105 66-67 0.22031099841698065 1105 68-69 0.28818162870810937 1105 70-71 0.06988841860101402 1105 72-73 0.2605456442636367 1105 74-75 0.3050533327610019 1105 76-77 0.13719967663533694 1105 78-79 0.09644389868415715 1105 80-81 0.28638026306598263 1105 82-83 0.10541407576769402 1105 84-85 0.272287212184537 1105 86-87 0.3356171757004631 1105 88-89 0.3696150547167498 1105 90-91 0.6978520253085563 1105 92-93 0.38596829697940294 1105 94-95 0.7734136268498659 1105 96-97 0.3687109380254796 1105 98-99 0.42530622931479556 1105 100-101 1.0142347123944973 1106 1 0.13058943565055614 1106 2 0.028462477948338005 1106 3 0.07233574147527833 1106 4 0.009614433208717799 1106 5 0.07686833638411628 1106 6 0.12725873892795647 1106 7 0.12955658570582784 1106 8 -0.02172345493476513 1106 9 0.17843216042641075 1106 10-11 0.24118549819169033 1106 12-13 0.16720491203368937 1106 14-15 0.24590756567119598 1106 16-17 0.2632187621028237 1106 18-19 0.2734857895872267 1106 20-21 0.1844747385960659 1106 22-23 0.1299225817395211 1106 24-25 0.10415562861866334 1106 26-27 0.06055873574793935 1106 28-29 -0.0121111030972898 1106 30-31 0.03146923815279479 1106 32-33 -0.0220784485822918 1106 34-35 -0.042477836392002644 1106 36-37 0.10727295755123833 1106 38-39 0.22996324717004768 1106 40-41 0.2151430708441353 1106 42-43 0.13313920508789323 1106 44-45 0.40439755169851566 1106 46-47 0.1949313089106255 1106 48-49 0.17688019304689107 1106 50-51 0.27323465533755353 1106 52-53 0.10325705810144115 1106 54-55 0.12304171557581611 1106 56-57 0.3281752830684539 1106 58-59 0.3370300030053599 1106 60-61 0.19853187338822664 1106 62-63 0.08501177386364844 1106 64-65 0.20701676835984273 1106 66-67 0.17482735613920752 1106 68-69 0.44041721883023044 1106 70-71 0.3649001299933836 1106 72-73 0.3017885114349035 1106 74-75 0.13497646154461407 1106 76-77 0.22714282251688545 1106 78-79 0.25728023805737266 1106 80-81 0.28728659277816604 1106 82-83 0.2539517155572355 1106 84-85 0.5763052275726785 1106 86-87 0.7347442078181743 1106 88-89 0.8185519939078638 1106 90-91 0.9443101110345573 1106 92-93 0.9037849042203092 1106 94-95 0.9411596877213029 1106 96-97 0.9406539866016956 1106 98-99 0.9094861190817518 1106 100-101 1.2064429875183897 1107 1 -0.03290222949776833 1107 2 -0.07032089841430533 1107 3 0.06377640400058837 1107 4 -0.07518963159995451 1107 5 -0.06776239651216542 1107 6 -0.050697649635296216 1107 7 0.04620738137562341 1107 8 0.11642434816403124 1107 9 0.04686560709126297 1107 10-11 0.027673722141202006 1107 12-13 0.16983720567348826 1107 14-15 0.09282612854956085 1107 16-17 0.2353715224530717 1107 18-19 0.10360710817485597 1107 20-21 0.17108809704797778 1107 22-23 0.1432136535302817 1107 24-25 0.030660422576453072 1107 26-27 0.05197120863260096 1107 28-29 0.2290994013253922 1107 30-31 0.30536116182074835 1107 32-33 0.2568267620321407 1107 34-35 0.33475490346917525 1107 36-37 0.3429765125399413 1107 38-39 0.11325950998001133 1107 40-41 0.1533692548949901 1107 42-43 0.28991039114983863 1107 44-45 0.7247267516971405 1107 46-47 0.6918899217052754 1107 48-49 0.5141090826156969 1107 50-51 0.5720585972806447 1107 52-53 0.5580584070788746 1107 54-55 0.518679540573153 1107 56-57 0.48341460280429516 1107 58-59 0.5874928975314191 1107 60-61 0.7206418336133211 1107 62-63 0.6896022162003632 1107 64-65 0.5826062584367477 1107 66-67 0.43221871429546255 1107 68-69 0.626870377616978 1107 70-71 0.6645151007552244 1107 72-73 0.47505716902358586 1107 74-75 0.4232461332728086 1107 76-77 0.23936578194787117 1107 78-79 0.2801451306101512 1107 80-81 0.43145804079749794 1107 82-83 0.2680142209287375 1107 84-85 0.5229418940445392 1107 86-87 0.37040100925653263 1107 88-89 0.6570366975900477 1107 90-91 0.8550988365533989 1107 92-93 0.9492805777980209 1107 94-95 0.9498647862647225 1107 96-97 1.0825761075627547 1107 98-99 1.249564568789026 1107 100-101 1.530348490342508 1108 1 0.14781798156758086 1108 2 0.09171835123099825 1108 3 -0.00461682956311904 1108 4 0.08262171900554449 1108 5 0.12314327579419881 1108 6 0.08952941640951906 1108 7 0.029939258865432805 1108 8 0.08013213471955538 1108 9 0.22195935648262832 1108 10-11 0.2710962828217376 1108 12-13 0.16266573305519927 1108 14-15 0.36578105842666275 1108 16-17 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2316 94-95 -1.2533888678312373 2316 96-97 -1.6041535660489963 2316 98-99 -2.3462704266593377 2316 100-101 -2.1882273072169944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7732.0 3 1569.0 4 1419.0 5 1522.0 6 1793.0 7 2128.0 8 2643.0 9 3089.0 10 3798.0 11 4710.0 12 6238.0 13 7929.0 14 8426.0 15 9852.0 16 11509.0 17 14128.0 18 12542.0 19 9251.0 20 9384.0 21 10051.0 22 10781.0 23 12446.0 24 13747.0 25 15798.0 26 18049.0 27 20725.0 28 24253.0 29 28582.0 30 32967.0 31 39200.0 32 47827.0 33 59031.0 34 77396.0 35 108053.0 36 150690.0 37 207614.0 38 170905.0 39 23830.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.942479559171524 19.170366605290884 14.303362427922728 22.58379140761486 2 16.10359697716058 21.450811610527822 38.66922773009642 23.77636368221518 3 18.768114662502867 25.442971027470808 30.944699356511695 24.844214953514633 4 13.152764970566999 17.273301161433057 36.67689095373349 32.89704291426646 5 13.625256244285236 37.126463757363474 33.9381413467451 15.31013865160619 6 30.87661562366521 35.69431038135949 17.837359710221943 15.591714284753355 7 27.012044422535634 33.67559300989429 21.67541803853744 17.63694452903263 8 25.811102857609146 33.512910094126376 20.90288637956257 19.773100668701908 9 26.541403998578 16.497643025768298 21.308324501858603 35.6526284737951 10-11 24.64579516032872 26.403217926477428 27.6513963436323 21.29959056956155 12-13 25.799593733010013 24.634824338512658 27.92608312284846 21.63949880562887 14-15 23.057472298240445 25.652819184416824 26.945532937594912 24.34417557974782 16-17 21.72967009199621 28.754211531105895 27.507762429881456 22.008355947016444 18-19 21.41562706733525 27.035624720587748 30.361361264955782 21.18738694712122 20-21 22.510796303022964 26.14803211311566 30.17591919925738 21.165252384604 22-23 22.26263878764885 26.263500210097906 29.987133343400085 21.486727658853155 24-25 22.1500175593179 26.48008226593009 30.070005951241875 21.299894223510137 26-27 21.83467025499472 26.569865454539332 30.092400008757497 21.503064281708454 28-29 21.548619877285947 26.80233373053839 30.161922444414795 21.48712394776087 30-31 21.76241744322808 26.602185242773192 29.964210495309075 21.67118681868965 32-33 21.514782332331954 26.851900897878604 29.826738604576082 21.806578165213363 34-35 22.11622882278408 26.74869809047655 29.534133884345547 21.60093920239382 36-37 22.207221115978395 26.45040922128532 29.45108448169025 21.891285181046037 38-39 21.83188578658183 26.610432613055636 29.729207256517753 21.82847434384479 40-41 21.85212378814491 26.478433736765794 29.792521652147702 21.876920822941592 42-43 21.84766257372103 26.70343804064196 29.134442230169654 22.31445715546736 44-45 21.792181602074933 26.77073058141406 29.08316998322836 22.35391783328265 46-47 21.97457407232683 26.842288822094325 28.860980442371716 22.322156663207128 48-49 22.132008437741323 26.974567548905238 28.519001137923873 22.374422875429563 50-51 21.807087310748077 27.165075700769226 28.449419079795433 22.578417908687264 52-53 22.115559195241786 27.199114006009694 28.176142278030746 22.509184520717778 54-55 21.70268996319672 26.962522320178028 28.33844361330142 22.99634410332384 56-57 21.893626438788097 27.31695894808171 28.07055520764327 22.718859405486924 58-59 22.085313564146006 26.72688476993395 28.30165244717794 22.886149218742105 60-61 21.903478337364927 26.74972293764803 28.64267016973936 22.704128555247685 62-63 21.850131536717825 26.74929514346052 28.58950351312124 22.811069806700413 64-65 21.655983329106064 26.781625951451836 28.45613062349851 23.10626009594359 66-67 21.60042539582753 26.90354731074921 28.168526860485255 23.32750043293801 68-69 21.49184978181248 26.58032149159696 28.4806344882917 23.447194238298863 70-71 21.53549767023232 26.539631332910275 28.29173966942497 23.633131327432437 72-73 21.521978283575834 26.42618577229144 28.155764003790324 23.896071940342402 74-75 21.391971269748044 26.760271427403787 28.00693988605981 23.840817416788358 76-77 21.45342814813317 26.383309090434224 28.1711333839675 23.992129377465098 78-79 21.27819783185278 26.466396750221726 28.192862515276502 24.062542902648996 80-81 21.38998220509419 26.455317939160377 28.056101336197365 24.098598519548073 82-83 21.35018786541086 26.133721420026625 28.177157924887535 24.338932789674985 84-85 21.198059818745115 25.947249479255518 28.09400148693874 24.760689215060623 86-87 21.430271314417322 25.743215665329604 28.223935800757253 24.60257721949582 88-89 21.402252571602364 25.637587624462437 28.059668673062028 24.900491130873174 90-91 21.243772185765298 25.560318948355228 28.187492758355543 25.008416107523935 92-93 21.52555526905206 25.330237410278063 28.15343441850745 24.990772902162426 94-95 21.30635755338693 25.008469434191234 28.1553066693634 25.529866343058433 96-97 21.410897972873894 24.774314304832437 28.256472662366445 25.55831505992722 98-99 21.326811328871752 24.783897856949334 28.026177906787137 25.863112907391777 100-101 21.590483536212666 24.08080855736977 28.44530135342286 25.883406552994703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3557.0 1 2331.5 2 1235.5 3 1296.5 4 1104.0 5 1135.0 6 1635.0 7 2614.0 8 3672.0 9 3877.0 10 3149.0 11 2536.5 12 2384.0 13 2415.0 14 2568.5 15 2601.0 16 2541.5 17 2611.0 18 2741.5 19 2865.5 20 3078.0 21 3376.5 22 3816.0 23 4284.0 24 4642.5 25 5349.5 26 6491.0 27 7842.5 28 9002.0 29 10347.0 30 12140.5 31 14040.5 32 16030.5 33 17706.5 34 19459.0 35 21284.5 36 23439.5 37 26050.0 38 27837.5 39 28588.0 40 29495.5 41 31668.0 42 33895.0 43 36129.5 44 38702.0 45 40561.0 46 41723.0 47 43810.0 48 48276.5 49 49960.5 50 47601.5 51 45421.5 52 43526.5 53 42789.5 54 42062.5 55 39206.5 56 35562.5 57 31418.0 58 27897.0 59 25110.5 60 21981.5 61 18549.0 62 15021.0 63 11702.5 64 8970.0 65 7066.5 66 5315.5 67 4153.5 68 3110.0 69 2068.5 70 1583.5 71 1244.5 72 1004.0 73 782.5 74 580.5 75 482.5 76 366.5 77 231.5 78 154.5 79 115.0 80 80.5 81 64.5 82 58.5 83 45.5 84 37.5 85 29.5 86 19.0 87 15.5 88 12.5 89 11.0 90 8.5 91 5.5 92 6.5 93 6.0 94 4.0 95 3.5 96 2.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27710408606842846 2 0.16784014904205236 3 0.19393929221809147 4 0.16439942598669027 5 0.23522796888243636 6 0.19184129035506584 7 0.23866869193779847 8 0.24244509529124464 9 0.14576916944302246 10-11 0.20115641862689976 12-13 0.20900294559461569 14-15 0.16947659049521235 16-17 0.2815098899807823 18-19 0.22914376347966198 20-21 0.19419105244165455 22-23 0.24156393450877386 24-25 0.23350760735475531 26-27 0.3403798222572822 28-29 0.31272815770260404 30-31 0.2863772543030018 32-33 0.18890408774682993 34-35 0.35410075444146993 36-37 0.3911095073052425 38-39 0.37168201005362494 40-41 0.3329948556994318 42-43 0.3662272052097582 44-45 0.37654937437584446 46-47 0.3821720193687532 48-49 0.40277439766366513 50-51 0.394424350248823 52-53 0.3939627898389574 54-55 0.3880044645479645 56-57 0.3666887656196239 58-59 0.3388692609159037 60-61 0.31054623576505735 62-63 0.3767172145248865 64-65 0.40877468299191855 66-67 0.41544632891634004 68-69 0.4209430937974673 70-71 0.42052349342486217 72-73 0.4115860054883729 74-75 0.4067606012034139 76-77 0.3914451876033266 78-79 0.36329000260152233 80-81 0.3753325332952896 82-83 0.3851931420515101 84-85 0.3866617433556281 86-87 0.3822978994805347 88-89 0.3906899069326374 90-91 0.41099856496672565 92-93 0.410201324258776 94-95 0.4187611718599206 96-97 0.41993605290321495 98-99 0.4162855296615503 100-101 0.3907738270071584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1191610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.437071592226886 #Duplication Level Percentage of deduplicated Percentage of total 1 78.618909904058 43.584021368540974 2 11.769744692551754 13.049603582864478 3 3.931409558883455 6.538374995225615 4 1.7989789154681801 3.989204917188647 5 0.9951144030175764 2.7583114201270744 6 0.6171884513136248 2.0529072216821427 7 0.4263214878397134 1.654381038987241 8 0.28421362645368986 1.2604776925759704 9 0.21844452704369127 1.0898932396186127 >10 1.1807456035615922 12.347962476289949 >50 0.10730211873654233 4.071228216069471 >100 0.04888547251502459 4.724464597740873 >500 0.0012183282476532593 0.41393914757749456 >1k 0.0012183282476532593 1.313326000349889 >5k 3.045820619133148E-4 1.1519040851616023 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7772 0.6522268191774154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5872 0.4927786775874657 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3943 0.3308968538364062 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3366 0.2824749708377741 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1951 0.16372806539052207 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1673 0.1403982846736768 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1294 0.10859257643020787 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.392007452102617E-5 2 0.0 0.0 0.0 0.0 8.392007452102617E-5 3 0.0 0.0 0.0 0.0 8.392007452102617E-5 4 0.0 0.0 0.0 8.392007452102617E-5 8.392007452102617E-5 5 0.0 0.0 0.0 8.392007452102617E-5 8.392007452102617E-5 6 0.0 0.0 0.0 8.392007452102617E-5 8.392007452102617E-5 7 0.0 0.0 0.0 8.392007452102617E-5 8.392007452102617E-5 8 0.0 0.0 0.0 8.392007452102617E-5 1.6784014904205235E-4 9 0.0 0.0 0.0 1.6784014904205235E-4 1.6784014904205235E-4 10-11 0.0 0.0 0.0 1.6784014904205235E-4 1.6784014904205235E-4 12-13 8.392007452102617E-5 0.0 0.0 2.0980018630256545E-4 1.6784014904205235E-4 14-15 8.392007452102617E-5 0.0 0.0 3.356802980841047E-4 2.0980018630256545E-4 16-17 8.392007452102617E-5 0.0 0.0 4.196003726051309E-4 2.5176022356307855E-4 18-19 8.392007452102617E-5 0.0 0.0 5.454804843866702E-4 2.5176022356307855E-4 20-21 8.392007452102617E-5 0.0 0.0 7.552806706892356E-4 2.5176022356307855E-4 22-23 8.392007452102617E-5 0.0 0.0 0.0016784014904205236 2.5176022356307855E-4 24-25 8.392007452102617E-5 0.0 0.0 0.0035666031671436125 2.5176022356307855E-4 26-27 8.392007452102617E-5 0.0 0.0 0.007846526967715947 2.5176022356307855E-4 28-29 1.6784014904205235E-4 0.0 0.0 0.02186117941272732 2.5176022356307855E-4 30-31 1.6784014904205235E-4 0.0 0.0 0.06088401406500449 2.5176022356307855E-4 32-33 1.6784014904205235E-4 0.0 0.0 0.12705499282483362 2.5176022356307855E-4 34-35 1.6784014904205235E-4 0.0 0.0 0.23686441033559635 2.5176022356307855E-4 36-37 1.6784014904205235E-4 0.0 0.0 0.4048723995266908 2.5176022356307855E-4 38-39 1.6784014904205235E-4 0.0 0.0 0.621931672275325 2.5176022356307855E-4 40-41 1.6784014904205235E-4 0.0 0.0 0.8562784803752905 2.5176022356307855E-4 42-43 3.356802980841047E-4 0.0 0.0 1.0992690561509217 2.937202608235916E-4 44-45 3.356802980841047E-4 0.0 0.0 1.3823734275476036 3.356802980841047E-4 46-47 3.356802980841047E-4 0.0 0.0 1.72145248864981 4.61560409865644E-4 48-49 3.356802980841047E-4 0.0 0.0 2.0682102365706903 5.874405216471832E-4 50-51 3.356802980841047E-4 0.0 0.0 2.4320037596193385 5.874405216471832E-4 52-53 3.356802980841047E-4 0.0 0.0 2.7901326776378177 7.133206334287225E-4 54-55 3.356802980841047E-4 0.0 0.0 3.181451985129363 8.392007452102618E-4 56-57 3.356802980841047E-4 0.0 0.0 3.639697552051426 8.392007452102618E-4 58-59 3.356802980841047E-4 0.0 0.0 4.130420187813127 8.811607824707748E-4 60-61 3.356802980841047E-4 0.0 0.0 4.65894881714655 9.65080856991801E-4 62-63 3.356802980841047E-4 0.0 0.0 5.1831975226794 0.0010070408942523142 64-65 3.356802980841047E-4 0.0 0.0 5.72532120408523 0.0010070408942523142 66-67 3.356802980841047E-4 0.0 0.0 6.343854113342452 0.0010070408942523142 68-69 3.356802980841047E-4 0.0 0.0 7.024361997633454 0.0010070408942523142 70-71 3.356802980841047E-4 0.0 0.0 7.758159129245307 0.0010070408942523142 72-73 3.356802980841047E-4 0.0 0.0 8.48771829709385 0.0011748810432943664 74-75 3.356802980841047E-4 0.0 0.0 9.244677369273504 0.0011748810432943664 76-77 3.356802980841047E-4 0.0 0.0 10.047330922029857 0.0011748810432943664 78-79 3.356802980841047E-4 0.0 0.0 10.92643566267487 0.0011748810432943664 80-81 3.356802980841047E-4 0.0 0.0 11.850563523300409 0.0011748810432943664 82-83 3.356802980841047E-4 0.0 0.0 12.768858938746739 0.0011748810432943664 84-85 3.356802980841047E-4 0.0 0.0 13.731170433279345 0.0012168410805548794 86-87 3.356802980841047E-4 0.0 0.0 14.713790585846041 0.0012588011178153926 88-89 3.356802980841047E-4 0.0 0.0 15.723936522855631 0.0012588011178153926 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5875 0.0 31.228008 1 GTATCAA 8410 0.0 28.65344 1 GTACATG 5130 0.0 23.903814 1 ATCAACG 10025 0.0 23.854996 3 TATCAAC 10080 0.0 23.771912 2 TCAACGC 10080 0.0 23.630692 4 CAACGCA 10165 0.0 23.386412 5 AACGCAG 10345 0.0 23.024391 6 GAGTACT 5385 0.0 22.345964 12-13 TACATGG 5385 0.0 21.852404 2 ACGCAGA 11260 0.0 20.985737 7 AGTACTT 5665 0.0 20.948212 12-13 CGCAGAG 11290 0.0 20.845915 8 ACATGGG 5320 0.0 20.78153 3 AGAGTAC 8770 0.0 20.025839 10-11 GAACGCG 60 0.004765756 19.7911 78-79 ACGATAC 145 6.190238E-4 19.634348 3 GTACTTT 6175 0.0 19.564413 14-15 GCAGAGT 11655 0.0 19.54087 9 GCGGTAT 150 7.688898E-4 19.01187 1 >>END_MODULE