FastQCFastQC Report
Thu 26 May 2016
SRR938774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191610
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70090.5881958023178724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60860.5107375735349653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42920.36018495984424437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38010.3189802032544205No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT21740.1824422420087109No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.15189533488305737No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16900.14182492594053422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.10758553553595555No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12290.10313777158634117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62650.030.9050371
ACCGCCG804.5255898E-429.6517188
GTATCAA86000.026.2755091
GTACATG56300.022.6455231
GAGTACT55600.021.84597612-13
TACATGG57400.021.744092
TATCAAC104850.021.7255212
TCAACGC104850.021.5897354
CAACGCA105300.021.5876085
ATCAACG105100.021.5835343
AACGCAG107700.021.057186
ACATGGG59000.020.671813
AGTACTT57300.020.61821212-13
GTACTTT62250.019.51222814-15
GTATTAC1507.642479E-419.0289971
ACGCAGA118100.018.8806867
AGAGTAC93000.018.7491110-11
CATGGGA35350.018.6604794
CGCAGAG119800.018.5731628
TCTAGGA5900.018.5000632
Produced by FastQC (version 0.11.5)