##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938774_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1191610 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00043722358826 31.0 31.0 34.0 30.0 34.0 2 32.19342318375979 33.0 31.0 34.0 30.0 34.0 3 32.264860986396556 34.0 31.0 34.0 30.0 34.0 4 35.91551766097968 37.0 35.0 37.0 35.0 37.0 5 35.81396765720328 37.0 35.0 37.0 35.0 37.0 6 35.84118797257492 37.0 35.0 37.0 35.0 37.0 7 35.754589169275185 37.0 35.0 37.0 33.0 37.0 8 35.74349073941978 37.0 35.0 37.0 33.0 37.0 9 37.372851016691705 39.0 37.0 39.0 34.0 39.0 10-11 37.30689277532079 39.0 37.0 39.0 33.5 39.0 12-13 37.22159011757202 39.0 37.0 39.0 33.0 39.0 14-15 38.41315824808452 40.0 38.0 41.0 33.0 41.0 16-17 38.34143301919252 40.0 38.0 41.0 33.0 41.0 18-19 38.460153070215924 40.0 38.0 41.0 33.5 41.0 20-21 38.45584251558815 40.0 38.0 41.0 34.0 41.0 22-23 38.3658697056923 40.0 38.0 41.0 34.0 41.0 24-25 38.2663950453588 40.0 38.0 41.0 33.0 41.0 26-27 38.07437668364649 40.0 37.0 41.0 33.0 41.0 28-29 37.877812371497384 40.0 37.0 41.0 33.0 41.0 30-31 37.61604677704953 40.0 36.5 41.0 32.0 41.0 32-33 37.422734367788124 40.0 36.0 41.0 32.0 41.0 34-35 37.355805171155 40.0 36.0 41.0 32.0 41.0 36-37 37.19686264801403 40.0 36.0 41.0 31.0 41.0 38-39 37.036639504535884 40.0 36.0 41.0 31.0 41.0 40-41 36.83649054640361 39.5 35.0 41.0 31.0 41.0 42-43 36.603979070333416 39.0 35.0 41.0 30.0 41.0 44-45 36.30018588296507 39.0 35.0 41.0 30.0 41.0 46-47 35.90828207215448 39.0 35.0 41.0 28.5 41.0 48-49 35.53588632186705 39.0 35.0 41.0 27.5 41.0 50-51 35.25965962017774 39.0 35.0 40.0 27.0 41.0 52-53 35.016210421194856 38.0 34.5 40.0 26.0 41.0 54-55 34.71374946500953 38.0 34.0 40.0 26.0 41.0 56-57 34.413629878903336 38.0 34.0 40.0 24.5 41.0 58-59 34.06546311293124 37.5 33.5 40.0 23.5 41.0 60-61 33.67182928978441 37.0 33.0 40.0 22.5 41.0 62-63 33.28292016683311 36.5 33.0 39.5 22.0 41.0 64-65 32.8555324309128 36.0 32.0 39.0 20.5 40.5 66-67 32.41973967992884 35.0 32.0 39.0 19.5 40.0 68-69 32.07521378638984 35.0 31.5 38.5 18.0 40.0 70-71 31.646590746972585 35.0 31.0 37.5 15.0 40.0 72-73 31.175394214550064 35.0 31.0 37.0 10.0 39.0 74-75 30.691951225652687 35.0 30.5 36.5 7.5 39.0 76-77 28.7584578847106 32.5 28.0 34.5 4.5 36.5 78-79 29.7089878399812 34.0 29.5 35.0 2.0 37.0 80-81 29.767802385008515 34.0 30.0 35.0 2.0 37.0 82-83 29.463110833242418 34.0 30.0 35.0 2.0 36.5 84-85 29.09510074604946 34.0 29.0 35.0 2.0 36.0 86-87 28.53768766626665 34.0 29.0 35.0 2.0 36.0 88-89 27.964989803710946 33.5 27.5 35.0 2.0 35.0 90-91 27.462219182450635 33.0 26.5 35.0 2.0 35.0 92-93 27.18072649608513 33.0 26.0 35.0 2.0 35.0 94-95 26.868458639991275 33.0 25.5 35.0 2.0 35.0 96-97 26.290893413113352 33.0 25.0 35.0 2.0 35.0 98-99 25.507190691585336 33.0 22.0 34.5 2.0 35.0 100-101 22.157141178741366 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21625760086767087 1101 2 0.17343048101575675 1101 3 0.03328130952007058 1101 4 0.10955868947170444 1101 5 -0.019084847061350274 1101 6 0.1546153846174576 1101 7 0.25031303632730584 1101 8 0.17260967997593468 1101 9 0.28369655833783725 1101 10-11 0.2878860567144912 1101 12-13 0.24443972602460207 1101 14-15 0.3737191873393755 1101 16-17 0.19841365089290974 1101 18-19 0.2861511760776452 1101 20-21 0.2576833236091076 1101 22-23 0.21639621257002517 1101 24-25 0.20310732578734303 1101 26-27 0.15892622601587902 1101 28-29 0.301976502420338 1101 30-31 0.31259204503769666 1101 32-33 0.3189009842922559 1101 34-35 0.38933830693016347 1101 36-37 0.47295107923471846 1101 38-39 0.5572000542814166 1101 40-41 0.6001947574058093 1101 42-43 0.4469200564544238 1101 44-45 0.41734778855043686 1101 46-47 0.4275171906665918 1101 48-49 0.4903178648218329 1101 50-51 0.4575950653498779 1101 52-53 0.3260359165513407 1101 54-55 0.5423716503683877 1101 56-57 0.6174159424346755 1101 58-59 0.31129564044844216 1101 60-61 0.5042387080576063 1101 62-63 0.24056531042928242 1101 64-65 0.4465544099929488 1101 66-67 0.5606056287253907 1101 68-69 0.4653515416529288 1101 70-71 0.5265178108979569 1101 72-73 0.3066563943747056 1101 74-75 0.287044531238152 1101 76-77 0.36620156133541926 1101 78-79 0.12594571068850513 1101 80-81 0.5857091464590134 1101 82-83 0.599999438549137 1101 84-85 0.36024991576664434 1101 86-87 0.48322137706947643 1101 88-89 0.5338199999703797 1101 90-91 0.36486844729501655 1101 92-93 0.2387149045583037 1101 94-95 0.309534694006107 1101 96-97 0.545257047731095 1101 98-99 0.057940701272098494 1101 100-101 0.21491687437590912 1102 1 0.2354213639338738 1102 2 0.27360469704363055 1102 3 0.27108618756885505 1102 4 0.11130084975044952 1102 5 -0.02866672859445174 1102 6 0.03963280622024001 1102 7 0.0743548481739964 1102 8 0.26668633502819716 1102 9 0.16958506007999574 1102 10-11 0.09102194521623375 1102 12-13 0.20828990024063643 1102 14-15 0.2709317308933805 1102 16-17 0.19144500977792234 1102 18-19 0.2752626743354796 1102 20-21 0.2764115466056225 1102 22-23 0.13103035891149517 1102 24-25 0.1739261411183577 1102 26-27 0.2726021842040325 1102 28-29 0.424798802071912 1102 30-31 0.2285328115882166 1102 32-33 0.22003338847344622 1102 34-35 0.30484353341100245 1102 36-37 0.48079080048906775 1102 38-39 0.5136460473127826 1102 40-41 0.5823376145486634 1102 42-43 0.5845507184753274 1102 44-45 0.6063721787943379 1102 46-47 0.6544335669732106 1102 48-49 0.7503352864246224 1102 50-51 0.7785880967087664 1102 52-53 0.5673251151576082 1102 54-55 0.5371451695321454 1102 56-57 0.6862312734451237 1102 58-59 0.5787172432359 1102 60-61 0.43498783697747 1102 62-63 0.40476391670105727 1102 64-65 0.4086624239302381 1102 66-67 0.545797266356054 1102 68-69 0.611693005067572 1102 70-71 0.5221624102010978 1102 72-73 0.4046529100541463 1102 74-75 0.39941386921724487 1102 76-77 0.4380656728336767 1102 78-79 0.6546913552878095 1102 80-81 0.7311795297342734 1102 82-83 0.7493896824515751 1102 84-85 0.8798492189025318 1102 86-87 0.8656255582541448 1102 88-89 0.8635238327229935 1102 90-91 0.7825513741242851 1102 92-93 0.6703351136175364 1102 94-95 0.7254754605566269 1102 96-97 0.4868946783931172 1102 98-99 0.9290208406449203 1102 100-101 1.377373320368939 1103 1 0.1884737739222615 1103 2 0.13470673440328795 1103 3 0.11377455864899133 1103 4 0.00350468250306335 1103 5 -0.014088234595227789 1103 6 0.052998442108744825 1103 7 0.04276307020264625 1103 8 0.09817769681681199 1103 9 0.1328908092089165 1103 10-11 0.2306200990102738 1103 12-13 0.1442231658232913 1103 14-15 0.23847492195029218 1103 16-17 0.04367291821772312 1103 18-19 -0.009125331374931989 1103 20-21 0.1960544037484837 1103 22-23 0.1361177088921366 1103 24-25 0.09193269841607332 1103 26-27 0.1401465850944703 1103 28-29 -0.031237359852212876 1103 30-31 0.08784580761996352 1103 32-33 0.24931589670030974 1103 34-35 0.12273182948145944 1103 36-37 0.27730738730672044 1103 38-39 0.059701385098300364 1103 40-41 0.09674219497840397 1103 42-43 0.03815511501036184 1103 44-45 0.037535675697164095 1103 46-47 0.11414006135958488 1103 48-49 0.1515448174931393 1103 50-51 0.19327214868320652 1103 52-53 0.10074827863031288 1103 54-55 -0.021167294172848017 1103 56-57 -0.2899607150372603 1103 58-59 -0.2431347699270887 1103 60-61 -0.11418282213983133 1103 62-63 -0.1369103114251473 1103 64-65 0.11677798722872268 1103 66-67 -0.17345027768188714 1103 68-69 -0.3231030169997986 1103 70-71 -0.2640932760164816 1103 72-73 -0.35917548715839587 1103 74-75 -0.14221347785979788 1103 76-77 -0.3317982813862166 1103 78-79 -0.4108634743676305 1103 80-81 -0.1447323822866302 1103 82-83 -0.2800591778852457 1103 84-85 -0.1673267271872838 1103 86-87 -0.15660664509467281 1103 88-89 -0.5855210037075125 1103 90-91 -0.47092084809793633 1103 92-93 -0.16207269706771754 1103 94-95 0.007673667583343757 1103 96-97 -0.12225650240440444 1103 98-99 -0.008333979331851538 1103 100-101 0.5646691609612731 1104 1 0.12887530218062437 1104 2 0.16459007254246671 1104 3 0.10893567456594866 1104 4 0.11963790049614431 1104 5 -0.013451460277657645 1104 6 0.08011241269697678 1104 7 0.11000583740757008 1104 8 0.09606012184564605 1104 9 -0.020331509130180336 1104 10-11 0.09762587803756873 1104 12-13 -0.012393279774556731 1104 14-15 0.12579290061235326 1104 16-17 0.23213108334651622 1104 18-19 0.16779730655278513 1104 20-21 0.17118416153395088 1104 22-23 0.1965331740978229 1104 24-25 0.2358527051442536 1104 26-27 0.2642304710998502 1104 28-29 -0.05418676633048847 1104 30-31 -0.08560460808553216 1104 32-33 -0.03648313492213617 1104 34-35 0.2615170923611174 1104 36-37 0.3299899493021172 1104 38-39 0.31045530186108294 1104 40-41 0.44409207242937754 1104 42-43 0.38740930810917007 1104 44-45 0.33033471933423186 1104 46-47 0.44177246235919654 1104 48-49 0.43881864246430524 1104 50-51 0.29150509995195506 1104 52-53 0.32987439598903023 1104 54-55 0.35517984394710567 1104 56-57 0.3392188673349068 1104 58-59 0.5099049263435802 1104 60-61 0.6946857620585476 1104 62-63 0.6413405416121449 1104 64-65 0.592916972234498 1104 66-67 0.4760339951939372 1104 68-69 0.6832587188710235 1104 70-71 0.7076874092968595 1104 72-73 0.8398945820457584 1104 74-75 0.7840078072228636 1104 76-77 0.7172468575026691 1104 78-79 0.6542256740368302 1104 80-81 0.8327357189244431 1104 82-83 0.8182909158287153 1104 84-85 0.8541629042044896 1104 86-87 1.0172492636904096 1104 88-89 0.8249431206677258 1104 90-91 0.79206694505854 1104 92-93 0.835674563601259 1104 94-95 0.630479499474923 1104 96-97 0.8649359235855982 1104 98-99 1.2524070817984843 1104 100-101 1.7195269680546978 1105 1 0.16133286102443378 1105 2 0.18388927907106734 1105 3 0.12572231828048075 1105 4 0.03166632404410308 1105 5 -0.030227961005053317 1105 6 -0.04615618519444098 1105 7 -0.006908028345648631 1105 8 0.09381997068616243 1105 9 0.10582841389903308 1105 10-11 0.11082625129949264 1105 12-13 0.044758830256569127 1105 14-15 0.20289336834895266 1105 16-17 0.15443942435954483 1105 18-19 0.2000346176758825 1105 20-21 0.005623080465404939 1105 22-23 0.10246856037175434 1105 24-25 0.12744445860590758 1105 26-27 -0.004844865212618288 1105 28-29 0.13119153810981743 1105 30-31 0.14220833204765881 1105 32-33 0.26918302749317036 1105 34-35 0.3491351132614682 1105 36-37 0.33255105355463144 1105 38-39 0.37787646187104684 1105 40-41 0.16406448350436165 1105 42-43 0.31495360398300676 1105 44-45 0.2314174107613738 1105 46-47 0.31121048291178255 1105 48-49 0.27669530953669863 1105 50-51 0.3152165058858927 1105 52-53 0.3364122756989971 1105 54-55 0.2921505973681988 1105 56-57 0.27325684847474463 1105 58-59 0.41800076887804494 1105 60-61 0.4162165706574825 1105 62-63 0.40132993494558633 1105 64-65 0.5201490090042711 1105 66-67 0.4320271964945519 1105 68-69 0.5822309444742402 1105 70-71 0.6600892686513049 1105 72-73 0.6785653001112877 1105 74-75 0.8251013951161958 1105 76-77 0.9027577452748652 1105 78-79 0.5290880192113754 1105 80-81 0.6113461876463688 1105 82-83 0.8497761969053776 1105 84-85 0.7054464267769802 1105 86-87 0.7939905247824903 1105 88-89 0.46993422381900984 1105 90-91 0.6606920776418725 1105 92-93 0.7800598406207264 1105 94-95 0.8559711445515141 1105 96-97 1.0498889470651669 1105 98-99 1.3661425700818874 1105 100-101 1.8332320273766811 1106 1 0.08982664390843098 1106 2 0.0855142782022753 1106 3 0.13608394661579837 1106 4 0.1207764667354283 1106 5 -0.11553763524068472 1106 6 -0.012101402289594887 1106 7 -0.07615189637181174 1106 8 0.09511969197587433 1106 9 -0.06391935676618488 1106 10-11 0.0906221881066287 1106 12-13 0.22595005609530716 1106 14-15 0.19899316192262972 1106 16-17 0.15507434168475953 1106 18-19 -0.003068032504479845 1106 20-21 0.027641540677940668 1106 22-23 0.1202535433780767 1106 24-25 0.14124617813023121 1106 26-27 0.13597055351310416 1106 28-29 0.32746300609984047 1106 30-31 0.2597420587725665 1106 32-33 0.30723393208528194 1106 34-35 0.2992039220356304 1106 36-37 0.30941427700243906 1106 38-39 0.3241600930282189 1106 40-41 0.36062856254409326 1106 42-43 0.3642144309393629 1106 44-45 0.3983680005012218 1106 46-47 0.6320616265970145 1106 48-49 0.6527258773133369 1106 50-51 0.6125743184619097 1106 52-53 0.3058517969567376 1106 54-55 0.4991722200041977 1106 56-57 0.48401742869257447 1106 58-59 0.4737191950337234 1106 60-61 0.48089195310220845 1106 62-63 0.6492667142779354 1106 64-65 0.42706498584560393 1106 66-67 0.32335520583585975 1106 68-69 0.44147599406520754 1106 70-71 0.5894459413648008 1106 72-73 0.7501865460366233 1106 74-75 0.6176881184979699 1106 76-77 0.5964999695358664 1106 78-79 0.4479294601644135 1106 80-81 0.23722106688349598 1106 82-83 0.42309715856012176 1106 84-85 0.3862265375660563 1106 86-87 0.5428339813460852 1106 88-89 0.6498142602390047 1106 90-91 0.608070046323455 1106 92-93 0.7006823167635439 1106 94-95 0.907791449395237 1106 96-97 0.9170185091216432 1106 98-99 1.3127414010277612 1106 100-101 1.6480359918741385 1107 1 0.1418145292384736 1107 2 0.030312086285825046 1107 3 0.013597207779064036 1107 4 -2.7535063695438566E-4 1107 5 -0.11009359683401954 1107 6 -0.08724467098156197 1107 7 0.05028070460382139 1107 8 -0.028921602409916147 1107 9 -0.04458951390624577 1107 10-11 -0.08695914072553279 1107 12-13 0.027161523091081108 1107 14-15 0.041328501762500025 1107 16-17 -0.0467019494523484 1107 18-19 0.004802033878682721 1107 20-21 -0.05938263022003554 1107 22-23 -0.1291649593394837 1107 24-25 -0.14782827346094507 1107 26-27 0.012920709415560339 1107 28-29 0.019689884578419026 1107 30-31 0.06889853534251245 1107 32-33 -0.07309554293422593 1107 34-35 -0.18744484535514516 1107 36-37 -0.228376686498855 1107 38-39 -0.1284854359916423 1107 40-41 -0.20773062848196844 1107 42-43 0.00958875155183847 1107 44-45 0.07775174745450641 1107 46-47 0.09340022199661036 1107 48-49 0.09766532115235549 1107 50-51 0.05473673892070963 1107 52-53 -0.11687008473615634 1107 54-55 -0.024864261516121644 1107 56-57 -0.10494873342536692 1107 58-59 -0.10554904314811608 1107 60-61 -0.09153067149084393 1107 62-63 -0.18875595431845937 1107 64-65 0.004395358120746096 1107 66-67 0.16503565219476002 1107 68-69 -0.017183554144232005 1107 70-71 -0.15000546072604237 1107 72-73 -0.013198892295100961 1107 74-75 0.05334354483190751 1107 76-77 0.0039080008332419425 1107 78-79 0.16760961087585713 1107 80-81 0.09303105495446218 1107 82-83 0.1436357182069976 1107 84-85 -0.06773874792280665 1107 86-87 0.09116855800299817 1107 88-89 0.2515355471992322 1107 90-91 0.1655256906114424 1107 92-93 0.5185964896210429 1107 94-95 0.7262931356170341 1107 96-97 0.9278044844316433 1107 98-99 1.0876512457440946 1107 100-101 1.8960401996936653 1108 1 0.13178488001026523 1108 2 0.006976990079394341 1108 3 -0.04234829397307038 1108 4 0.00994681631861738 1108 5 0.060536288209675604 1108 6 -0.04551252082072921 1108 7 -0.024529287141135114 1108 8 0.03185856631651518 1108 9 0.05372037521253503 1108 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quality scores pass #Quality Count 2 2.0 3 6.0 4 17.0 5 54.0 6 182.0 7 333.0 8 678.0 9 1157.0 10 1978.0 11 3056.0 12 4184.0 13 5807.0 14 6562.0 15 8328.0 16 10469.0 17 13857.0 18 12983.0 19 8970.0 20 8307.0 21 8941.0 22 9300.0 23 10166.0 24 11523.0 25 13260.0 26 15621.0 27 18533.0 28 21825.0 29 27080.0 30 32450.0 31 38993.0 32 47896.0 33 59973.0 34 79153.0 35 111680.0 36 160921.0 37 240021.0 38 182726.0 39 14618.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.44838564513212 20.06712284405715 14.821531460678884 22.662960050131847 2 16.237023858477187 22.051258381517442 37.92197111471035 23.789746645295022 3 18.623039417259 25.65403110077962 31.24864678879835 24.474282693163033 4 13.37014627268989 18.065558362215825 35.96571025755071 32.59858510754358 5 13.510584537794246 37.57909652420198 33.87295532651817 15.037363611485596 6 30.964080193291043 36.18214944808157 17.384528492217417 15.469241866409975 7 26.922734787388492 33.99610610854222 21.200057065650675 17.88110203841861 8 25.859383523132568 34.053507439514604 20.499072683176543 19.588036354176282 9 26.218141841709958 16.54425525129866 21.074093033794615 36.16350987319677 10-11 24.627436829163905 26.693087503461705 27.349048765955303 21.330426901419088 12-13 25.68780893077433 24.66398402161781 27.86863990735224 21.779567140255622 14-15 22.8329738756808 25.83764822383162 26.67814973019696 24.651228170290615 16-17 21.722572201495748 29.03996673320306 27.191242540881692 22.0462185244195 18-19 21.456013292939804 27.144451624273042 30.034239390404576 21.365295692382574 20-21 22.418492627621454 26.288970384605705 29.964040248067743 21.328496739705106 22-23 22.171683688455115 26.46948246490043 29.788563372244276 21.57027047440018 24-25 22.169100569861897 26.77411003399198 29.79373609280596 21.26305330334016 26-27 21.832356138827777 26.816782429213365 29.92367770330688 21.427183728651976 28-29 21.43440387375064 26.926888831077285 30.092941482532037 21.54576581264004 30-31 21.762909005463197 26.65108550616393 29.969956613321475 21.6160488750514 32-33 21.477328991868145 27.023187116590158 29.72532959609268 21.774154295449012 34-35 22.051388786314227 26.869549089248906 29.56680575790739 21.512256366529478 36-37 22.254909448921335 26.538092556237185 29.390470419085684 21.816527575755796 38-39 21.756821747001755 26.645373231037556 29.775894353672467 21.821910668288226 40-41 21.788320187725656 26.507310793050255 29.820918721035046 21.883450298189043 42-43 21.89931385644069 26.754479278263084 29.164982885227165 22.181223980069063 44-45 21.74932714313409 26.84461811019327 29.10341979974168 22.302634946930958 46-47 22.013996295739933 26.889202006376294 28.646501639391037 22.450300058492733 48-49 22.12627146985503 27.009899012532486 28.374805458450876 22.48902405916161 50-51 21.771352108220015 27.202242009651652 28.40229957884693 22.6241063032814 52-53 22.094183499634948 27.32676798616997 28.158877485083206 22.420171029111874 54-55 21.63820377472495 27.060573509789275 28.12295969318821 23.178263022297564 56-57 21.88545209267391 27.525401824021955 27.77955643579313 22.809589647511007 58-59 21.921166763454973 27.013462526141236 28.10019100305135 22.965179707352444 60-61 21.86930115418281 27.19647627224045 28.19301112221682 22.74121145135992 62-63 21.619236680811532 27.228047030942047 28.19091329839707 22.96180298984935 64-65 21.42474699707197 27.256423576289436 28.043399265013804 23.27543016162479 66-67 21.43477292077814 27.372373500199533 27.69838195904689 23.494471619975435 68-69 21.31119682176827 26.964659459586475 28.054281364813537 23.669862353831718 70-71 21.4133159450793 26.993879703829027 27.793093204230583 23.799711146861092 72-73 21.393073237049034 26.97010766945561 27.63114609645773 24.00567299703762 74-75 21.191436081114194 27.20404597500512 27.43881356558051 24.165704378300173 76-77 21.184805768685777 26.923388578086133 27.752290785427142 24.13951486780095 78-79 21.031942952200914 26.959295331440536 27.65424078920996 24.35452092714859 80-81 21.083501174890902 27.007091305807318 27.509944612286002 24.399462907015774 82-83 21.045613136986272 26.7474126120607 27.590020959258478 24.61695329169455 84-85 20.828207215448007 26.59498493634662 27.445598811691745 25.131209036513624 86-87 20.909932531938328 26.423352635925333 27.684545857027526 24.982168975108813 88-89 20.884515297743867 26.35119543888903 27.412773805613806 25.351515457753298 90-91 20.73256107834878 26.32245155855097 27.591449031171017 25.353538331929233 92-93 20.749334196035683 26.241844504615415 27.513407342788653 25.495413956560252 94-95 20.517318860982094 25.897035554132643 27.687896707425402 25.897748877459865 96-97 20.59855993152122 25.785575817591326 27.64826579166002 25.967598459227432 98-99 20.329596092681328 25.866013209019727 27.48525104690293 26.31913965139601 100-101 20.40237011151911 25.327849901329273 27.994959673612428 26.27482031353918 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2672.0 1 1761.0 2 994.0 3 1134.0 4 990.0 5 949.5 6 1383.5 7 2385.5 8 3275.5 9 3407.0 10 2844.0 11 2288.5 12 2199.0 13 2214.5 14 2268.0 15 2327.0 16 2427.0 17 2498.5 18 2545.0 19 2723.5 20 2907.5 21 3249.0 22 3732.5 23 4104.5 24 4638.0 25 5415.0 26 6511.0 27 7829.5 28 8957.0 29 10385.0 30 12123.0 31 13986.0 32 16131.0 33 17859.5 34 19444.0 35 21182.5 36 23507.0 37 26231.5 38 28098.0 39 28863.0 40 29763.5 41 32192.5 42 34725.5 43 37005.5 44 39240.0 45 41241.0 46 42595.0 47 44790.0 48 49651.0 49 51236.0 50 49026.5 51 46780.0 52 43876.0 53 42680.5 54 41957.5 55 38863.0 56 35142.5 57 31058.0 58 27700.5 59 24893.0 60 21590.0 61 18049.5 62 14814.0 63 11670.0 64 8697.0 65 6598.0 66 4800.5 67 3843.5 68 2835.5 69 1773.5 70 1315.0 71 1017.5 72 795.0 73 640.0 74 519.0 75 402.5 76 277.0 77 155.0 78 84.5 79 56.0 80 37.0 81 29.0 82 27.5 83 18.5 84 12.5 85 10.0 86 5.5 87 4.0 88 3.0 89 1.5 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23036060456021684 2 0.0 3 0.0 4 0.0 5 0.028868505635233004 6 0.0032728829063200207 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0026434823474123246 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.6156040986564394E-4 26-27 0.0015525213786389842 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.005538724918387728 36-37 0.01535737363734779 38-39 0.013678972146927265 40-41 0.007049286259766199 42-43 0.008014367116757999 44-45 0.004112083651530283 46-47 7.552806706892356E-4 48-49 0.0022238819748071938 50-51 4.196003726051309E-5 52-53 0.0 54-55 0.0 56-57 0.0010490009315128272 58-59 5.035204471261571E-4 60-61 0.002601522310151811 62-63 9.65080856991801E-4 64-65 0.001930161713983602 66-67 0.0064198857008585025 68-69 3.356802980841047E-4 70-71 8.392007452102617E-5 72-73 0.0 74-75 5.035204471261571E-4 76-77 0.058534251978415754 78-79 0.13934928374216396 80-81 8.392007452102617E-4 82-83 0.0010490009315128272 84-85 0.0 86-87 0.10649457456718221 88-89 0.3507859114978894 90-91 0.38687154354193065 92-93 4.6156040986564394E-4 94-95 3.776403353446178E-4 96-97 0.0 98-99 0.0 100-101 0.0017203615276810368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1191610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.134268522931904 #Duplication Level Percentage of deduplicated Percentage of total 1 76.94434193516364 40.88381325703274 2 12.688241312376741 13.483608419511672 3 4.213786184431161 6.716893398653578 4 1.940989269608957 4.125321802061271 5 1.0720353983884028 2.848090836202884 6 0.6693610964768679 2.133960734340361 7 0.4460261193125438 1.6589490114255776 8 0.3243329785656858 1.3786556459161174 9 0.22612739935204362 1.081360256180737 >10 1.2976029438974144 12.991386015485801 >50 0.11908290094740251 4.346144264296995 >100 0.054274810748592986 5.022525517822616 >500 0.0020570608123528476 0.6895175056217969 >1k 0.0014241190239365868 1.538781781232792 >5k 3.164708942081304E-4 1.1009915542150261 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7009 0.5881958023178724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6086 0.5107375735349653 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4292 0.36018495984424437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3801 0.3189802032544205 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2174 0.1824422420087109 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1810 0.15189533488305737 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1690 0.14182492594053422 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 0.10758553553595555 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1229 0.10313777158634117 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.6784014904205235E-4 0.0 5 0.0 0.0 0.0 1.6784014904205235E-4 0.0 6 0.0 0.0 0.0 1.6784014904205235E-4 8.392007452102617E-5 7 0.0 0.0 0.0 1.6784014904205235E-4 8.392007452102617E-5 8 0.0 0.0 0.0 1.6784014904205235E-4 8.392007452102617E-5 9 0.0 0.0 0.0 3.356802980841047E-4 8.392007452102617E-5 10-11 0.0 0.0 0.0 3.356802980841047E-4 8.392007452102617E-5 12-13 0.0 0.0 0.0 3.776403353446178E-4 8.392007452102617E-5 14-15 0.0 0.0 0.0 5.035204471261571E-4 2.0980018630256545E-4 16-17 0.0 0.0 0.0 5.874405216471832E-4 2.5176022356307855E-4 18-19 0.0 0.0 0.0 7.133206334287225E-4 2.5176022356307855E-4 20-21 0.0 0.0 0.0 9.231208197312879E-4 2.5176022356307855E-4 22-23 0.0 0.0 0.0 0.001888201676723089 2.5176022356307855E-4 24-25 0.0 0.0 0.0 0.003860323427967204 3.776403353446178E-4 26-27 0.0 0.0 0.0 0.008014367116757999 4.196003726051309E-4 28-29 0.0 0.0 0.0 0.02240665989711399 4.196003726051309E-4 30-31 0.0 0.0 0.0 0.06197497503377783 4.196003726051309E-4 32-33 0.0 0.0 0.0 0.1304957158801957 4.196003726051309E-4 34-35 0.0 0.0 0.0 0.24257097540302616 4.196003726051309E-4 36-37 0.0 0.0 0.0 0.4126350064198857 4.196003726051309E-4 38-39 0.0 0.0 0.0 0.6335126425592266 4.196003726051309E-4 40-41 0.0 0.0 0.0 0.8736079757638824 4.196003726051309E-4 42-43 0.0 0.0 0.0 1.1205847550792627 4.196003726051309E-4 44-45 0.0 0.0 0.0 1.4068780893077433 5.03520447126157E-4 46-47 0.0 0.0 0.0 1.7523770361108082 6.294005589076963E-4 48-49 0.0 0.0 0.0 2.1045056688010337 7.552806706892356E-4 50-51 0.0 0.0 0.0 2.4729147959483386 7.552806706892356E-4 52-53 0.0 0.0 0.0 2.8362467585871216 9.231208197312879E-4 54-55 0.0 0.0 0.0 3.2330208709225334 0.0010909609687733402 56-57 0.0 0.0 0.0 3.695756161831472 0.0010909609687733402 58-59 0.0 0.0 0.0 4.190800681431005 0.0010909609687733402 60-61 0.0 0.0 0.0 4.7258750765770685 0.0010909609687733402 62-63 4.196003726051309E-5 0.0 0.0 5.2586416696738025 0.0010909609687733402 64-65 8.392007452102617E-5 0.0 0.0 5.8080663975629605 0.0010909609687733402 66-67 8.392007452102617E-5 0.0 0.0 6.434949354235027 0.0010909609687733402 68-69 8.392007452102617E-5 0.0 0.0 7.1241849262762145 0.0010909609687733402 70-71 8.392007452102617E-5 0.0 0.0 7.867968546756069 0.0012588011178153926 72-73 8.392007452102617E-5 0.0 0.0 8.61225568768305 0.001426641266857445 74-75 8.392007452102617E-5 0.0 0.0 9.377984407650153 0.001426641266857445 76-77 8.392007452102617E-5 0.0 0.0 10.193184011547402 0.0014686013041179582 78-79 8.392007452102617E-5 0.0 0.0 11.086177524525642 0.0015105613413784712 80-81 8.392007452102617E-5 0.0 0.0 12.018193872156159 0.0015944814158994974 82-83 8.392007452102617E-5 0.0 0.0 12.947650657513783 0.0017623215649415498 84-85 8.392007452102617E-5 0.0 0.0 13.92456424501305 0.0017623215649415498 86-87 1.2588011178153925E-4 0.0 0.0 14.918597527714605 0.0017623215649415498 88-89 1.6784014904205235E-4 0.0 0.0 15.945905119963747 0.0017623215649415498 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6265 0.0 30.905037 1 ACCGCCG 80 4.5255898E-4 29.651718 8 GTATCAA 8600 0.0 26.275509 1 GTACATG 5630 0.0 22.645523 1 GAGTACT 5560 0.0 21.845976 12-13 TACATGG 5740 0.0 21.74409 2 TATCAAC 10485 0.0 21.725521 2 TCAACGC 10485 0.0 21.589735 4 CAACGCA 10530 0.0 21.587608 5 ATCAACG 10510 0.0 21.583534 3 AACGCAG 10770 0.0 21.05718 6 ACATGGG 5900 0.0 20.67181 3 AGTACTT 5730 0.0 20.618212 12-13 GTACTTT 6225 0.0 19.512228 14-15 GTATTAC 150 7.642479E-4 19.028997 1 ACGCAGA 11810 0.0 18.880686 7 AGAGTAC 9300 0.0 18.74911 10-11 CATGGGA 3535 0.0 18.660479 4 CGCAGAG 11980 0.0 18.573162 8 TCTAGGA 590 0.0 18.500063 2 >>END_MODULE