FastQCFastQC Report
Thu 26 May 2016
SRR938773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5095763
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT176110.3456008452512411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156420.3069609006541317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107960.21186228637399349No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT101570.1993224567155105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78580.15420654375017048No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT73560.14435522217183178No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC68360.13415066595522596No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT55230.1083841615082962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG187500.025.9908541
TACATGG193800.024.8878822
ACATGGG191200.024.2103253
GAGTACT142850.020.65011212-13
CATGGGG93750.020.114344
AGAGTAC248850.019.29338610-11
AGTACTT155550.019.16197612-13
CATGGGA108350.018.7158184
GTATCAA404850.018.3369161
GTACTTT162000.018.25353214-15
GAGTACA155650.017.42111
AGTACAT143900.017.1542172
CCGTCTA4004.5292836E-1016.572059
TCAACGC444350.016.5592354
CAACGCA443150.016.5399255
ATCAACG447700.016.4035783
AACGCAG449350.016.3737776
TATCAAC451200.016.2973352
TGGACCG7000.015.5676765
TACTTTT190800.015.56032514-15
Produced by FastQC (version 0.11.5)