##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938773_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5095763 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.534315077055194 33.0 31.0 34.0 30.0 34.0 2 31.602972704970778 33.0 31.0 34.0 28.0 34.0 3 31.62539525484211 33.0 31.0 34.0 28.0 34.0 4 35.17741641438191 37.0 35.0 37.0 33.0 37.0 5 35.21495465939056 37.0 35.0 37.0 33.0 37.0 6 35.29677891220608 37.0 35.0 37.0 32.0 37.0 7 35.2050181690161 37.0 35.0 37.0 32.0 37.0 8 35.17642578746304 37.0 35.0 37.0 32.0 37.0 9 36.73414835030593 39.0 37.0 39.0 32.0 39.0 10-11 36.622826159693844 39.0 37.0 39.0 32.0 39.0 12-13 36.50930007930118 39.0 37.0 39.0 32.0 39.0 14-15 37.64924899372282 40.0 37.0 41.0 32.0 41.0 16-17 37.59488569228985 40.0 37.0 41.0 32.0 41.0 18-19 37.532519663885466 40.0 37.0 41.0 32.0 41.0 20-21 37.42231654023156 40.0 37.0 41.0 31.5 41.0 22-23 37.33783596293627 40.0 37.0 41.0 31.5 41.0 24-25 37.350586948411845 40.0 37.0 41.0 31.0 41.0 26-27 37.26552667382686 40.0 37.0 41.0 31.0 41.0 28-29 37.16121717199171 40.0 37.0 41.0 31.0 41.0 30-31 36.97678522333162 39.5 36.0 41.0 30.0 41.0 32-33 36.79332427744383 39.0 36.0 41.0 30.0 41.0 34-35 36.473487777983394 39.0 35.5 41.0 30.0 41.0 36-37 36.23654514544731 39.0 35.0 41.0 29.5 41.0 38-39 36.083995958995736 39.0 35.0 41.0 29.0 41.0 40-41 35.93382934017929 39.0 35.0 41.0 28.5 41.0 42-43 35.696711267772855 39.0 35.0 40.0 27.0 41.0 44-45 35.68508190039451 39.0 35.0 41.0 27.0 41.0 46-47 35.53187942610361 39.0 35.0 41.0 26.5 41.0 48-49 35.32899018262819 39.0 35.0 41.0 26.0 41.0 50-51 34.205616705486506 38.0 33.5 40.0 24.0 40.5 52-53 34.326389786966146 38.0 33.5 39.5 24.0 40.5 54-55 34.87726548507064 39.0 34.0 40.0 24.5 41.0 56-57 34.797082792115724 38.5 34.0 40.0 24.0 41.0 58-59 34.58884950104626 38.0 34.0 40.0 24.0 41.0 60-61 34.31150290937785 38.0 34.0 40.0 23.0 41.0 62-63 33.97467238959112 37.5 33.5 40.0 22.5 41.0 64-65 33.59543291161697 37.0 33.0 40.0 22.0 41.0 66-67 33.200242731068926 36.5 32.5 39.5 20.5 41.0 68-69 32.80499289311532 36.0 32.0 39.0 20.0 41.0 70-71 32.395372096386744 35.5 32.0 39.0 18.5 40.0 72-73 31.97879630587215 35.0 31.5 38.0 17.5 40.0 74-75 31.52566220210791 35.0 31.0 37.0 13.5 39.5 76-77 31.08740310332329 35.0 31.0 37.0 9.5 39.0 78-79 30.668703685787584 35.0 30.5 36.0 7.5 39.0 80-81 30.21702598806106 34.0 30.0 36.0 4.5 37.5 82-83 29.74948275655677 34.0 29.5 35.0 2.0 37.0 84-85 29.337988344434386 34.0 29.0 35.0 2.0 37.0 86-87 28.96445026976333 34.0 29.0 35.0 2.0 36.0 88-89 28.591186050057665 34.0 29.0 35.0 2.0 36.0 90-91 28.172858706340932 34.0 28.5 35.0 2.0 35.5 92-93 27.724467268199092 34.0 27.0 35.0 2.0 35.0 94-95 27.21703285651236 34.0 26.0 35.0 2.0 35.0 96-97 26.64211581268595 33.0 25.0 35.0 2.0 35.0 98-99 25.950875070131794 33.0 24.5 35.0 2.0 35.0 100-101 23.724317732202223 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3389786218715152 1101 2 0.2411943071933358 1101 3 0.39165745468293167 1101 4 0.2947923364683689 1101 5 0.21736799782188854 1101 6 0.13928949233918786 1101 7 0.3073249552091255 1101 8 0.36230127932420686 1101 9 0.48886207614558685 1101 10-11 0.4194163429238742 1101 12-13 0.39831493374687454 1101 14-15 0.5788997000530358 1101 16-17 0.591322035990892 1101 18-19 0.6098033496598703 1101 20-21 0.5859113120399044 1101 22-23 0.5578384728380286 1101 24-25 0.5602978813763073 1101 26-27 0.5642732758826057 1101 28-29 0.4876330441493337 1101 30-31 0.4813319904933877 1101 32-33 0.5391823747701991 1101 34-35 0.42171784182659877 1101 36-37 0.5806374578938005 1101 38-39 0.6149998494459297 1101 40-41 0.5247436208464009 1101 42-43 0.577832869155614 1101 44-45 0.5785898766456938 1101 46-47 0.6815725790795923 1101 48-49 0.5508764451256809 1101 50-51 0.44567148787159994 1101 52-53 0.4149449164802732 1101 54-55 0.44500748021211933 1101 56-57 0.3940537553520045 1101 58-59 0.3930013201296063 1101 60-61 0.2993584843072554 1101 62-63 0.38626725731104017 1101 64-65 0.32601450753099925 1101 66-67 0.11972791583468023 1101 68-69 0.15527447846350384 1101 70-71 0.06667989470323477 1101 72-73 -0.023154461348703137 1101 74-75 -0.08538573566860919 1101 76-77 -0.07657591320485224 1101 78-79 -0.13704332497901106 1101 80-81 -0.23200304838914576 1101 82-83 -0.19888591908072684 1101 84-85 -0.21439734300430047 1101 86-87 -0.24015013258593143 1101 88-89 -0.262374812237784 1101 90-91 -0.4798604514315983 1101 92-93 -0.28293655805049767 1101 94-95 -0.31628706048056543 1101 96-97 -0.4276077441620991 1101 98-99 -0.4991817157084739 1101 100-101 -0.16636462712647315 1102 1 0.38126156661478916 1102 2 0.33731357760379055 1102 3 0.4006823503791601 1102 4 0.3235984592358676 1102 5 0.2713429035171515 1102 6 0.289067232613462 1102 7 0.3673223036655173 1102 8 0.30679118819411855 1102 9 0.4561926063205277 1102 10-11 0.4220746182954187 1102 12-13 0.5012686392704921 1102 14-15 0.42554844307387185 1102 16-17 0.46478764378952064 1102 18-19 0.5466539272881903 1102 20-21 0.5639409535875757 1102 22-23 0.5149545358411771 1102 24-25 0.5346595471219686 1102 26-27 0.5072374752416451 1102 28-29 0.5441894959586477 1102 30-31 0.6648253040070315 1102 32-33 0.6314765234000248 1102 34-35 0.6264462292593151 1102 36-37 0.6613262795279269 1102 38-39 0.5443407359053225 1102 40-41 0.6150355624863693 1102 42-43 0.5704106051764484 1102 44-45 0.6986203976963026 1102 46-47 0.6743042238290045 1102 48-49 0.7006932039103333 1102 50-51 0.631705849715722 1102 52-53 0.6171593327136193 1102 54-55 0.4743176492420247 1102 56-57 0.44690932289039154 1102 58-59 0.4224583231032497 1102 60-61 0.3581012590224333 1102 62-63 0.4874934504456334 1102 64-65 0.49577176180099514 1102 66-67 0.5406758778335572 1102 68-69 0.4373452071028865 1102 70-71 0.3474456256803009 1102 72-73 0.29471620077912775 1102 74-75 0.1544409166666867 1102 76-77 0.32534439350293454 1102 78-79 0.13647984155404913 1102 80-81 0.06512157896263915 1102 82-83 0.07499664652268123 1102 84-85 -0.004201090187677892 1102 86-87 -0.020965687199325345 1102 88-89 -0.024591079063334575 1102 90-91 0.04722531948669584 1102 92-93 -0.13440327243122852 1102 94-95 -0.28811102233950336 1102 96-97 -0.27344648863390475 1102 98-99 -0.281222708812745 1102 100-101 -0.17375314652579377 1103 1 0.18089869690325955 1103 2 0.28763438280965303 1103 3 0.22736493060580543 1103 4 0.35903603145504803 1103 5 0.3287538585334744 1103 6 0.3319071442273227 1103 7 0.25724608075404376 1103 8 0.3522710276394747 1103 9 0.46783788597347353 1103 10-11 0.2938473077884183 1103 12-13 0.36317720218480076 1103 14-15 0.4650984542235008 1103 16-17 0.46017651240635615 1103 18-19 0.5280084550507596 1103 20-21 0.5273785359427805 1103 22-23 0.487232252802805 1103 24-25 0.45349583003871174 1103 26-27 0.495442182411864 1103 28-29 0.5407657999583222 1103 30-31 0.4168076571867019 1103 32-33 0.3733410959638306 1103 34-35 0.4831433546824897 1103 36-37 0.5967557429267458 1103 38-39 0.3383759383217537 1103 40-41 0.45569344121896194 1103 42-43 0.44112560041250504 1103 44-45 0.4675648826848544 1103 46-47 0.47039602581160267 1103 48-49 0.5020970434367769 1103 50-51 0.568745167358486 1103 52-53 0.47514043916216053 1103 54-55 0.45213369051591457 1103 56-57 0.457585345108555 1103 58-59 0.44447326360478456 1103 60-61 0.2871016948715308 1103 62-63 0.2616683779730522 1103 64-65 0.1940154419891229 1103 66-67 0.18693239022992003 1103 68-69 0.0777463909906615 1103 70-71 0.16345674490023043 1103 72-73 0.2074158804807169 1103 74-75 0.15363941045330165 1103 76-77 0.2197612172779948 1103 78-79 0.25738818147580034 1103 80-81 0.18257733521149788 1103 82-83 0.1950307542483607 1103 84-85 0.24670785789560057 1103 86-87 0.19647359800816133 1103 88-89 0.12722022868666727 1103 90-91 0.0805903278995963 1103 92-93 0.06387233623710031 1103 94-95 0.04596173405126436 1103 96-97 0.13725639405173595 1103 98-99 0.20390090001776073 1103 100-101 0.3900992526714333 1104 1 0.17329046645058455 1104 2 0.16065413589607402 1104 3 0.19403735858934112 1104 4 0.1275150438007273 1104 5 0.0739061219079602 1104 6 0.17550302900098558 1104 7 0.11612591614499479 1104 8 0.13412287949132207 1104 9 0.21085434687881843 1104 10-11 0.14773537363203815 1104 12-13 0.20796732564159015 1104 14-15 0.1963605941411899 1104 16-17 0.2991625206368127 1104 18-19 0.2882035167791557 1104 20-21 0.3300867663954534 1104 22-23 0.36997916638304673 1104 24-25 0.2686982991745097 1104 26-27 0.30519773796741845 1104 28-29 0.2799863760900081 1104 30-31 0.3539722662402056 1104 32-33 0.3570246350584796 1104 34-35 0.3310437662051271 1104 36-37 0.37926026967571147 1104 38-39 0.384150423918463 1104 40-41 0.23079467578686064 1104 42-43 0.44797086790222806 1104 44-45 0.44370397733505484 1104 46-47 0.4746910875400019 1104 48-49 0.4351381957001479 1104 50-51 0.3646822043955211 1104 52-53 0.5409112210551683 1104 54-55 0.5381209333142749 1104 56-57 0.44838699119909364 1104 58-59 0.36840741998337734 1104 60-61 0.419391406805687 1104 62-63 0.39901446850802813 1104 64-65 0.2507479522771803 1104 66-67 0.3113216906414422 1104 68-69 0.34519906588777616 1104 70-71 0.35337567494138256 1104 72-73 0.308479254966322 1104 74-75 0.1756842664203795 1104 76-77 0.06629743126976351 1104 78-79 0.1185021732453464 1104 80-81 0.049340298174463726 1104 82-83 0.04068301762284676 1104 84-85 0.15847493608490026 1104 86-87 0.08405713710281049 1104 88-89 -0.027370717815387025 1104 90-91 -0.03000308773826177 1104 92-93 -0.08225811643779579 1104 94-95 -0.07094402726560745 1104 96-97 0.06045269034803269 1104 98-99 0.03441571483257633 1104 100-101 0.26272517859736055 1105 1 0.1142904664505835 1105 2 0.12845413589607446 1105 3 0.11123735858934225 1105 4 0.27091504380072706 1105 5 0.1773061219079608 1105 6 0.15410302900098571 1105 7 0.20052591614498994 1105 8 0.1355228794913259 1105 9 0.16885434687882395 1105 10-11 0.14133537363203885 1105 12-13 0.16876732564158914 1105 14-15 0.26046059414119327 1105 16-17 0.19296252063681152 1105 18-19 0.23830351677915473 1105 20-21 0.22348676639546028 1105 22-23 0.28587916638305444 1105 24-25 0.16659829917451674 1105 26-27 0.1804977379674213 1105 28-29 0.15368637609000757 1105 30-31 0.11007226624019495 1105 32-33 0.14592463505847775 1105 34-35 0.20464376620512326 1105 36-37 0.20986026967571547 1105 38-39 0.2783504239184609 1105 40-41 0.1645946757868657 1105 42-43 0.094770867902227 1105 44-45 0.15070397733505558 1105 46-47 0.18129108754000356 1105 48-49 0.23023819570015291 1105 50-51 0.31588220439552117 1105 52-53 0.22351122105516907 1105 54-55 0.2964209333142662 1105 56-57 0.2531869911990938 1105 58-59 0.259507419983386 1105 60-61 0.219691406805687 1105 62-63 0.16881446850802462 1105 64-65 0.17124795227718437 1105 66-67 0.08392169064143928 1105 68-69 0.06879906588778084 1105 70-71 0.0510756749413801 1105 72-73 0.0911792549663133 1105 74-75 0.0977842664203834 1105 76-77 0.05999743126976753 1105 78-79 -0.03469782675465183 1105 80-81 0.007740298174461202 1105 82-83 0.06278301762284855 1105 84-85 0.02777493608490289 1105 86-87 0.02805713710280955 1105 88-89 -0.029170717815389935 1105 90-91 0.10539691226173531 1105 92-93 0.1813418835622045 1105 94-95 0.3044559727343916 1105 96-97 0.26045269034803553 1105 98-99 0.3713157148325763 1105 100-101 0.5880251785973591 1106 1 0.1957908265946422 1106 2 0.20298570852512654 1106 3 0.19697845502791722 1106 4 0.266099597622258 1106 5 0.1644639450372125 1106 6 0.1012679749793719 1106 7 0.15487525588088857 1106 8 0.1918779815321372 1106 9 0.20664106156469586 1106 10-11 0.2706019602666956 1106 12-13 0.21286448450513262 1106 14-15 0.30525291106796004 1106 16-17 0.2993326686960387 1106 18-19 0.3879861498323791 1106 20-21 0.33970729460673965 1106 22-23 0.24493014677520364 1106 24-25 0.21793975575715052 1106 26-27 0.14097372836357636 1106 28-29 0.20234499953939178 1106 30-31 0.19765653994969057 1106 32-33 0.21163876070873755 1106 34-35 0.2264353628437803 1106 36-37 0.32920032569812463 1106 38-39 0.34053593812414107 1106 40-41 0.3467239274875382 1106 42-43 0.49406282468493146 1106 44-45 0.37254255276522485 1106 46-47 0.40348296429070274 1106 48-49 0.475575570650129 1106 50-51 0.5367946093575071 1106 52-53 0.35299849596513155 1106 54-55 0.30851080926464647 1106 56-57 0.5047229655888543 1106 58-59 0.449134510819718 1106 60-61 0.4448074932402548 1106 62-63 0.25582147130915445 1106 64-65 0.19597684383380454 1106 66-67 0.2379108062877009 1106 68-69 0.39769486420710365 1106 70-71 0.40060740763446034 1106 72-73 0.2593891389199001 1106 74-75 0.0953474516944901 1106 76-77 0.021554774206940408 1106 78-79 0.2799967310684792 1106 80-81 0.21192685279631007 1106 82-83 0.2942162309081624 1106 84-85 0.2868386015510893 1106 86-87 0.4314299262184562 1106 88-89 0.5174895462902533 1106 90-91 0.6159295253069565 1106 92-93 0.696628278120027 1106 94-95 0.6700256806175453 1106 96-97 0.7575322821847692 1106 98-99 0.7841318812991602 1106 100-101 1.0416554306981993 1107 1 0.05348329262323048 1107 2 0.06384522773683088 1107 3 0.09768631404854489 1107 4 0.07491606863320754 1107 5 0.03734798237307757 1107 6 0.043455650207128826 1107 7 -0.039916732652798714 1107 8 0.065455161714425 1107 9 0.060311579831129336 1107 10-11 0.0821606002776818 1107 12-13 0.14340051444726498 1107 14-15 0.16827218263153298 1107 16-17 0.13825105039636298 1107 18-19 0.23609409620367217 1107 20-21 0.15939583222122877 1107 22-23 0.18902035676538986 1107 24-25 0.1347949487606357 1107 26-27 0.2066146082866922 1107 28-29 0.06741428306675346 1107 30-31 0.056542624931566365 1107 32-33 0.3328086713217857 1107 34-35 0.30745559119526433 1107 36-37 0.32168935915147756 1107 38-39 0.29821278103316473 1107 40-41 0.33518316612978794 1107 42-43 0.4169103239526777 1107 44-45 0.5022569138742767 1107 46-47 0.43827670046865563 1107 48-49 0.42007670575139144 1107 50-51 0.6198933593028926 1107 52-53 0.5568648276771597 1107 54-55 0.48922101214753866 1107 56-57 0.4444480081482425 1107 58-59 0.47935775502477185 1107 60-61 0.42479243163815994 1107 62-63 0.35508624619821916 1107 64-65 0.3792662810119083 1107 66-67 0.3475024553241397 1107 68-69 0.3824797123205741 1107 70-71 0.5574798531045673 1107 72-73 0.44526262114684556 1107 74-75 0.5391777232591721 1107 76-77 0.4179096898428831 1107 78-79 0.3911583419485396 1107 80-81 0.3309758519781667 1107 82-83 0.36352830733510544 1107 84-85 0.32919247648695205 1107 86-87 0.27172753521081106 1107 88-89 0.3246534839977784 1107 90-91 0.3574162264123082 1107 92-93 0.525971012454594 1107 94-95 0.45762037951405077 1107 96-97 0.4632629780894604 1107 98-99 0.5832900151873233 1107 100-101 0.61511859601951 1108 1 0.12665607940355272 1108 2 0.06160232402251964 1108 3 0.06513173021617291 1108 4 0.04796870609833803 1108 5 0.1704458289241515 1108 6 0.16885676839959274 1108 7 0.08530386525833222 1108 8 0.16669034286834972 1108 9 0.05712497139693795 1108 10-11 0.10905056252949663 1108 12-13 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-2.650279610876993 2316 68-69 -2.791075087290931 2316 70-71 -2.6130226731313684 2316 72-73 -2.608929955779555 2316 74-75 -2.6998591842720927 2316 76-77 -2.650002134890755 2316 78-79 -2.6293913191624654 2316 80-81 -2.5843445675021606 2316 82-83 -2.394273348137208 2316 84-85 -2.481786101792622 2316 86-87 -2.62043273441396 2316 88-89 -2.760819641559756 2316 90-91 -3.076023578310597 2316 92-93 -3.2317308846673996 2316 94-95 -3.1696453287840463 2316 96-97 -3.2087174581585565 2316 98-99 -3.4740800468894335 2316 100-101 -3.765703504866682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15514.0 3 4831.0 4 4961.0 5 5657.0 6 6380.0 7 8136.0 8 9722.0 9 11904.0 10 14854.0 11 19175.0 12 23874.0 13 29888.0 14 31316.0 15 29250.0 16 35032.0 17 43583.0 18 38358.0 19 30533.0 20 31671.0 21 34518.0 22 38282.0 23 43650.0 24 50100.0 25 57603.0 26 66538.0 27 78099.0 28 91473.0 29 109091.0 30 129232.0 31 155893.0 32 190590.0 33 240088.0 34 313079.0 35 432545.0 36 636660.0 37 960042.0 38 916079.0 39 157506.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15180691063862 19.449079866987287 14.224136406038642 23.17497681633545 2 14.724025677475089 21.14262839179501 40.41760762475106 23.715738305978846 3 18.938316498501667 25.348843101673452 30.520027285606567 25.19281311421831 4 12.298824021209422 16.949562406395515 38.76964220888514 31.981971363509924 5 13.150577862448284 37.98568552360305 34.89296562179673 13.970770992151932 6 30.596281673727795 37.05614867808265 18.20002194803107 14.147547700158485 7 27.353396356914267 33.23764554644421 22.010625388596665 17.39833270804486 8 25.412023861995443 34.81960673059839 20.868703070901777 18.8996663365044 9 26.103739421431705 17.126870177002356 21.38446843829361 35.38492196327233 10-11 24.295197591065147 26.710071340399477 28.464382563198953 20.53034850533642 12-13 25.6847376583477 24.276809872418692 28.41953577846666 21.61891669076695 14-15 22.447939633416798 25.566133049463485 27.14920679921169 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22.067697848836918 27.189807040841934 28.998108769081917 21.74438634123923 42-43 21.746492978070762 27.24588383260919 28.639604705993676 22.368018483326367 44-45 22.014992133087983 27.060560682473962 28.60231968673908 22.322127497698972 46-47 22.263406009503278 27.372480395532595 28.353563837898747 22.01054975706538 48-49 22.205066066699665 27.419100088930254 28.26954740219151 22.106286442178575 50-51 21.649108199700727 27.594689981258725 28.59130677206709 22.164895046973463 52-53 22.142110690687964 27.767030587919812 27.978630060276714 22.112228661115505 54-55 21.737016554107306 27.260300021815574 28.333817521445166 22.668865902631957 56-57 22.064165493092744 27.52611602371176 28.13688696346876 22.272831519726736 58-59 22.490846634457487 27.183029768182404 28.054177321410467 22.271946275949645 60-61 22.107150758809034 27.28760272060488 28.641620416002812 21.963626104583273 62-63 21.89378576780621 27.296349586807978 28.597370734204603 22.212493911181213 64-65 21.689732693085123 27.55704552401424 28.25777221230736 22.49544957059328 66-67 21.8028163570128 27.479576018112844 27.909136821894908 22.808470802979443 68-69 21.64417886957341 27.26863126110317 28.33464406004131 22.752545809282115 70-71 21.920636313703604 27.132505859927758 28.188979095564402 22.757878730804237 72-73 21.881597634839732 26.860530559781154 28.41567431402346 22.842197491355655 74-75 21.567869243860997 27.512781231963462 28.066130144461887 22.853219379713654 76-77 21.721394093166072 27.003307313617732 28.18426402439079 23.09103456882541 78-79 21.68180175968246 27.07990828474618 28.19162953439312 23.046660421178235 80-81 21.835739422339557 27.04981762764857 28.090851382080746 23.023591567931128 82-83 21.747974531675133 26.922898742946 28.298894424890797 23.03023230048807 84-85 21.56484099124522 26.98198873948775 28.172304643753876 23.280865625513155 86-87 21.55879359119721 26.927596269161512 28.279058185868056 23.234551953773217 88-89 21.557753854589883 26.775534621533808 28.030235139521203 23.636476384355106 90-91 21.608201911778654 26.57777344849147 28.226725424897214 23.58729921483266 92-93 21.90004144859487 26.64436750666786 28.177610581183444 23.27798046355383 94-95 21.64601287392633 26.382458252421443 28.201037151191482 23.770491722460747 96-97 21.468861879281054 26.210941158954732 28.463232850887444 23.856964110876774 98-99 21.49393123525795 26.337033050977464 28.010503259666308 24.158532454098275 100-101 21.54541023807937 25.692792950166066 28.497866524605847 24.263930287148714 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3457.0 1 2044.5 2 813.0 3 1000.0 4 878.5 5 911.5 6 1525.0 7 2925.0 8 4628.0 9 5385.5 10 4918.0 11 4695.5 12 5180.5 13 5659.0 14 6123.0 15 6537.5 16 7018.0 17 7336.5 18 7573.0 19 7980.5 20 8742.0 21 9983.0 22 11513.0 23 13815.0 24 16628.5 25 20927.5 26 27803.5 27 35865.5 28 43406.5 29 51996.0 30 62177.0 31 72840.0 32 83849.5 33 94679.5 34 107107.0 35 118419.5 36 130932.0 37 144179.0 38 151117.5 39 153902.0 40 156163.5 41 161435.5 42 166203.5 43 172744.5 44 180963.0 45 185039.5 46 188602.5 47 201520.0 48 230407.0 49 233880.0 50 208174.0 51 190457.5 52 175221.0 53 165122.5 54 158001.0 55 143997.5 56 128866.5 57 113419.5 58 98961.0 59 86785.0 60 72776.5 61 57332.5 62 44602.0 63 34503.5 64 25898.5 65 19544.0 66 14387.5 67 10703.5 68 7754.5 69 5170.0 70 3535.5 71 2441.5 72 1715.5 73 1283.0 74 997.0 75 761.0 76 526.5 77 320.0 78 211.5 79 134.5 80 83.0 81 68.0 82 54.0 83 41.5 84 35.5 85 30.0 86 24.5 87 20.0 88 19.5 89 14.0 90 12.5 91 12.5 92 8.0 93 7.0 94 6.0 95 5.5 96 5.5 97 3.5 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15952468747074774 2 0.09348943426136576 3 0.08701346589313513 4 0.10194744143320637 5 0.12225843313356606 6 0.03752136824259684 7 0.041485445849816796 8 0.04529253028447359 9 0.045685013215881504 10-11 0.043673538192415935 12-13 0.04831464885631455 14-15 0.04609712029385982 16-17 0.04489023527978048 18-19 0.04951172179710869 20-21 0.05119939840216274 22-23 0.06399434196606082 24-25 0.08102810118916441 26-27 0.08250972425522929 28-29 0.07265840267689058 30-31 0.061276397666061 32-33 0.05009063412093537 34-35 0.2951373523454682 36-37 0.46954695498986115 38-39 0.4477347160768662 40-41 0.42298866725159706 42-43 0.4529841752844471 44-45 0.46253132259094465 46-47 0.46143237038300244 48-49 0.47829932436025774 50-51 0.4671233728884173 52-53 0.4739820121147706 54-55 0.4650628374985258 56-57 0.4432015382191048 58-59 0.4226354326133299 60-61 0.39209044847650887 62-63 0.45017792232488046 64-65 0.48693394885123187 66-67 0.4883665115508708 68-69 0.49885561789274735 70-71 0.4966479014035778 72-73 0.4870124454375135 74-75 0.48000662511188213 76-77 0.46155011526242484 78-79 0.43632327484618105 80-81 0.44131762014834675 82-83 0.45349440309527744 84-85 0.45119837794654105 86-87 0.4621879000259627 88-89 0.4732264824718104 90-91 0.4780442104548426 92-93 0.4792412833956367 94-95 0.4839412664992465 96-97 0.4852658963927482 98-99 0.4785348141191025 100-101 0.4662108500728939 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5095763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.62331295217624 #Duplication Level Percentage of deduplicated Percentage of total 1 68.44729972111895 29.17450676744659 2 14.574482314825405 12.424254416415224 3 6.20474640123482 7.93400542946163 4 3.335665174033311 5.68708402465989 5 1.971356548895384 4.201287356194503 6 1.2696670275333586 3.2470449035768225 7 0.8786362891947211 2.6215272667839797 8 0.6121671282207153 2.087407286814924 9 0.4246600348283976 1.6290375806495567 >10 2.0576365106144103 15.190127572338607 >50 0.13041988462167617 3.866317093701583 >100 0.0848008465575654 6.85789492593006 >500 0.005595173461453587 1.5768376606543684 >1k 0.0024970164909907124 1.890847311381718 >5k 1.8496418451730495E-4 0.5434491006154263 >10k+ 1.8496418451730495E-4 1.0683713033750335 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17611 0.3456008452512411 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15642 0.3069609006541317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10796 0.21186228637399349 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10157 0.1993224567155105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7858 0.15420654375017048 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7356 0.14435522217183178 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6836 0.13415066595522596 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5523 0.1083841615082962 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.9624146570395835E-5 0.0 0.0 0.0 0.0 3 1.9624146570395835E-5 0.0 0.0 0.0 0.0 4 1.9624146570395835E-5 0.0 0.0 1.9624146570395835E-5 0.0 5 5.887243971118751E-5 0.0 0.0 1.9624146570395835E-5 0.0 6 9.812073285197918E-5 0.0 0.0 1.9624146570395835E-5 0.0 7 1.5699317256316668E-4 0.0 0.0 1.9624146570395835E-5 0.0 8 1.7661731913356252E-4 0.0 0.0 1.9624146570395835E-5 0.0 9 1.7661731913356252E-4 0.0 0.0 1.9624146570395835E-5 0.0 10-11 2.1586561227435422E-4 0.0 0.0 4.906036642598959E-5 0.0 12-13 2.4530183212994797E-4 0.0 0.0 7.849658628158334E-5 0.0 14-15 3.4342256498192715E-4 0.0 0.0 7.849658628158334E-5 0.0 16-17 3.5323463826712504E-4 0.0 0.0 1.079328061371771E-4 0.0 18-19 3.826708581227188E-4 1.9624146570395835E-5 0.0 1.8642939241876046E-4 0.0 20-21 4.1210707797831254E-4 1.9624146570395835E-5 0.0 3.924829314079167E-4 0.0 22-23 5.004157375450938E-4 1.9624146570395835E-5 0.0 7.653417162454376E-4 0.0 24-25 6.868451299638543E-4 1.9624146570395835E-5 0.0 0.0011970729407941461 9.812073285197918E-6 26-27 6.966572032490522E-4 1.9624146570395835E-5 0.0 0.0022567768555955213 1.9624146570395835E-5 28-29 8.536503758122189E-4 1.9624146570395835E-5 0.0 0.006112921656678303 1.9624146570395835E-5 30-31 9.223348888086043E-4 1.9624146570395835E-5 0.0 0.018485946069312878 1.9624146570395835E-5 32-33 9.321469620938022E-4 1.9624146570395835E-5 0.0 0.04401696075739786 1.9624146570395835E-5 34-35 9.910194018049898E-4 1.9624146570395835E-5 0.0 0.08207799303068058 1.9624146570395835E-5 36-37 0.0010597039148013752 1.9624146570395835E-5 0.0 0.14250074032092935 1.9624146570395835E-5 38-39 0.0011774487942237501 1.9624146570395835E-5 0.0 0.23596073836243953 1.9624146570395835E-5 40-41 0.0011774487942237501 1.9624146570395835E-5 0.0 0.3413424054454652 1.9624146570395835E-5 42-43 0.0012265091606497398 1.9624146570395835E-5 0.0 0.45167916953751575 1.9624146570395835E-5 44-45 0.0012559453805053335 1.9624146570395835E-5 0.0 0.5876941294169293 1.9624146570395835E-5 46-47 0.0013148178202165212 1.9624146570395835E-5 0.0 0.751879551698146 1.9624146570395835E-5 48-49 0.0013540661133573128 1.9624146570395835E-5 0.0 0.9257200540919976 1.9624146570395835E-5 50-51 0.0013540661133573128 1.9624146570395835E-5 0.0 1.1163980742432487 1.9624146570395835E-5 52-53 0.0013540661133573128 1.9624146570395835E-5 1.9624146570395835E-5 1.3110696082215756 1.9624146570395835E-5 54-55 0.0013540661133573128 1.9624146570395835E-5 1.9624146570395835E-5 1.5218820027540527 1.9624146570395835E-5 56-57 0.0013736902599277086 1.9624146570395835E-5 1.9624146570395835E-5 1.7781144845237113 1.9624146570395835E-5 58-59 0.0013736902599277086 1.9624146570395835E-5 1.9624146570395835E-5 2.048515600117195 1.9624146570395835E-5 60-61 0.0013835023332129065 1.9624146570395835E-5 1.9624146570395835E-5 2.346380708835949 1.9624146570395835E-5 62-63 0.0013933144064981044 1.9624146570395835E-5 1.9624146570395835E-5 2.648857491998745 1.9624146570395835E-5 64-65 0.0013933144064981044 1.9624146570395835E-5 1.9624146570395835E-5 2.965807083257208 1.9624146570395835E-5 66-67 0.001452186846209292 1.9624146570395835E-5 1.9624146570395835E-5 3.3260181056301086 1.9624146570395835E-5 68-69 0.0014718109927796877 1.9624146570395835E-5 1.9624146570395835E-5 3.7311684236492164 1.9624146570395835E-5 70-71 0.0015306834324908753 1.9624146570395835E-5 1.9624146570395835E-5 4.169198999247022 1.9624146570395835E-5 72-73 0.0016189920920576565 1.9624146570395835E-5 1.9624146570395835E-5 4.61014376061053 1.9624146570395835E-5 74-75 0.0016484283119132502 1.9624146570395835E-5 1.9624146570395835E-5 5.073420800771151 1.9624146570395835E-5 76-77 0.0016582403851984483 2.9436219855593753E-5 1.9624146570395835E-5 5.557577932882671 1.9624146570395835E-5 78-79 0.001687676605054042 3.924829314079167E-5 1.9624146570395835E-5 6.114393467671083 1.9624146570395835E-5 80-81 0.0017759852646208232 3.924829314079167E-5 1.9624146570395835E-5 6.710368986940719 1.9624146570395835E-5 82-83 0.0018250456310468129 3.924829314079167E-5 1.9624146570395835E-5 7.310387080403857 1.9624146570395835E-5 84-85 0.0018839180707580002 3.924829314079167E-5 1.9624146570395835E-5 7.924642492203818 1.9624146570395835E-5 86-87 0.0019427905104691878 3.924829314079167E-5 1.9624146570395835E-5 8.558011822763342 1.9624146570395835E-5 88-89 0.0019427905104691878 3.924829314079167E-5 1.9624146570395835E-5 9.234014611747053 1.9624146570395835E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18750 0.0 25.990854 1 TACATGG 19380 0.0 24.887882 2 ACATGGG 19120 0.0 24.210325 3 GAGTACT 14285 0.0 20.650112 12-13 CATGGGG 9375 0.0 20.11434 4 AGAGTAC 24885 0.0 19.293386 10-11 AGTACTT 15555 0.0 19.161976 12-13 CATGGGA 10835 0.0 18.715818 4 GTATCAA 40485 0.0 18.336916 1 GTACTTT 16200 0.0 18.253532 14-15 GAGTACA 15565 0.0 17.4211 1 AGTACAT 14390 0.0 17.154217 2 CCGTCTA 400 4.5292836E-10 16.57205 9 TCAACGC 44435 0.0 16.559235 4 CAACGCA 44315 0.0 16.539925 5 ATCAACG 44770 0.0 16.403578 3 AACGCAG 44935 0.0 16.373777 6 TATCAAC 45120 0.0 16.297335 2 TGGACCG 700 0.0 15.567676 5 TACTTTT 19080 0.0 15.560325 14-15 >>END_MODULE