FastQCFastQC Report
Thu 26 May 2016
SRR938773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5095763
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT191040.37489969608084206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150570.2954807749104501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117410.23040710488301752No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT92790.18209245602670296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82710.16231131628374398No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT70660.13866421966641698No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC65880.12928387760576776No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT54740.1074225783263468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG3400.023.8173681
GTACATG192650.023.613381
TACATGG198150.022.3179782
ACATGGG197200.021.4149023
GAGTACT148700.020.3114912-13
AGTACTT160250.019.17326412-13
AGAGTAC262150.018.57152410-11
GTACTTT165250.018.3059814-15
CATGGGG94000.017.71794
CATGGGA111100.016.6564924
GTATCAA415350.016.5720941
GAGTACA169200.016.3005391
AGTACAT152800.016.1167342
GTGTACG4902.910383E-1115.5542011
ACTTTTT196350.015.50292716-17
TACTTTT197400.015.44472914-15
GTATAGG13900.015.0786231
CAACGCA459400.014.9041885
TCAACGC460550.014.8978814
ATCAACG460250.014.8972833
Produced by FastQC (version 0.11.5)