##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938773_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5095763 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.999878722774195 33.0 31.0 34.0 30.0 34.0 2 32.21654598143595 34.0 31.0 34.0 30.0 34.0 3 32.31594424622966 34.0 31.0 34.0 30.0 34.0 4 35.943379823590696 37.0 35.0 37.0 35.0 37.0 5 35.850864139482155 37.0 35.0 37.0 35.0 37.0 6 35.876257000178384 37.0 35.0 37.0 35.0 37.0 7 35.80802207637993 37.0 35.0 37.0 33.0 37.0 8 35.77441847275864 37.0 35.0 37.0 33.0 37.0 9 37.42815531256065 39.0 37.0 39.0 34.0 39.0 10-11 37.351791380407604 39.0 37.0 39.0 34.0 39.0 12-13 37.28846465190787 39.0 37.0 39.0 33.0 39.0 14-15 38.50770797228992 40.0 38.0 41.0 34.0 41.0 16-17 38.452924223516675 40.0 38.0 41.0 33.0 41.0 18-19 38.474860585156726 40.0 38.0 41.0 33.5 41.0 20-21 38.545176159095305 40.0 38.0 41.0 34.0 41.0 22-23 38.501969773712005 40.0 38.0 41.0 34.0 41.0 24-25 38.43332235035264 40.0 38.0 41.0 34.0 41.0 26-27 38.28630020666189 40.0 38.0 41.0 33.5 41.0 28-29 38.14202151866168 40.0 38.0 41.0 33.0 41.0 30-31 37.94034583241018 40.0 37.0 41.0 33.0 41.0 32-33 37.75147333186413 40.0 37.0 41.0 33.0 41.0 34-35 37.729982143989034 40.0 37.0 41.0 33.0 41.0 36-37 37.66392648166722 40.0 37.0 41.0 32.5 41.0 38-39 37.57959779526638 40.0 37.0 41.0 32.0 41.0 40-41 37.45494521625123 40.0 37.0 41.0 32.0 41.0 42-43 37.302824817402225 40.0 37.0 41.0 31.0 41.0 44-45 37.08288277928153 40.0 36.0 41.0 31.0 41.0 46-47 36.83410315589638 40.0 36.0 41.0 30.5 41.0 48-49 36.59698910644 39.5 36.0 41.0 30.0 41.0 50-51 36.382848947252846 39.0 35.5 41.0 30.0 41.0 52-53 36.206829575865285 39.0 35.0 41.0 29.5 41.0 54-55 35.987243029159714 39.0 35.0 40.5 29.0 41.0 56-57 35.74371355182727 38.5 35.0 40.0 28.0 41.0 58-59 35.45943208112308 38.0 34.5 40.0 28.0 41.0 60-61 35.12894428174937 38.0 34.0 40.0 27.5 41.0 62-63 34.7878885850853 37.5 34.0 40.0 26.5 41.0 64-65 34.43973336279572 37.0 34.0 40.0 26.0 41.0 66-67 34.05412447556921 36.5 33.0 39.0 26.0 41.0 68-69 33.62260735045959 36.0 33.0 39.0 26.0 40.0 70-71 33.149451907398365 35.5 32.5 38.5 25.0 40.0 72-73 32.80955678276246 35.0 32.0 38.0 24.5 40.0 74-75 32.38839747845415 35.0 32.0 37.0 23.5 39.0 76-77 30.29606969554903 33.0 29.5 35.5 20.5 37.5 78-79 31.37939519557719 34.5 31.0 36.0 21.5 38.5 80-81 31.4268604132492 35.0 31.0 36.0 22.0 37.5 82-83 31.11415052073654 34.5 31.0 35.5 20.5 37.0 84-85 30.7261097111463 34.0 31.0 35.0 19.5 37.0 86-87 30.211199873306512 34.0 30.5 35.0 17.0 36.0 88-89 29.703154660057777 34.0 30.0 35.0 9.0 36.0 90-91 29.294524784610275 34.0 29.0 35.0 4.5 35.5 92-93 29.01645357132975 34.0 29.0 35.0 2.0 35.0 94-95 28.64076704509217 34.0 29.0 35.0 2.0 35.0 96-97 28.13904276945376 34.0 29.0 35.0 2.0 35.0 98-99 27.430759534931276 33.0 28.0 35.0 2.0 35.0 100-101 24.344280532669984 30.0 20.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06426408316010424 1101 2 0.10659128942355522 1101 3 0.11481643056340829 1101 4 0.07972252846413141 1101 5 0.03056288045272737 1101 6 0.13209469111309602 1101 7 0.09644951684833813 1101 8 0.08744632146576947 1101 9 0.19792434480564225 1101 10-11 0.17750579430559554 1101 12-13 0.197531940917699 1101 14-15 0.24133496529785248 1101 16-17 0.11586135494935235 1101 18-19 0.16758716586033273 1101 20-21 0.24958188098910483 1101 22-23 0.19313292724841347 1101 24-25 0.23728355275425628 1101 26-27 0.29739834741690885 1101 28-29 0.23958935879934984 1101 30-31 0.2307424486537215 1101 32-33 0.27808112308271404 1101 34-35 0.23789324045719695 1101 36-37 0.20201633335157254 1101 38-39 0.24207570687771351 1101 40-41 0.2817915449086641 1101 42-43 0.2450256509211286 1101 44-45 0.28829436567337297 1101 46-47 0.35148830908641315 1101 48-49 0.25041871062877874 1101 50-51 0.25839469658627223 1101 52-53 0.31805046837649087 1101 54-55 0.2900652071420211 1101 56-57 0.11952046419639828 1101 58-59 0.24919548491808996 1101 60-61 0.29308828338089654 1101 62-63 0.1264533997837134 1101 64-65 0.15098733590603786 1101 66-67 0.09656597601652805 1101 68-69 0.08749265798665107 1101 70-71 0.039322219270232495 1101 72-73 0.04217109565783517 1101 74-75 0.11679030813648694 1101 76-77 0.1285327870803208 1101 78-79 0.11346927314746225 1101 80-81 0.06975470173291853 1101 82-83 0.11104344481050887 1101 84-85 0.06689036099350787 1101 86-87 0.16259076054072708 1101 88-89 0.26021749585273923 1101 90-91 0.28958960415804924 1101 92-93 0.25745200374016974 1101 94-95 0.21484438709766707 1101 96-97 0.15609940288087287 1101 98-99 0.21008026917261802 1101 100-101 0.6865529518199267 1102 1 0.05985863607314457 1102 2 0.0824740767893104 1102 3 0.11122415495929516 1102 4 0.08448694584659933 1102 5 0.07588455248250625 1102 6 0.08961661094043194 1102 7 0.06898833211944577 1102 8 0.0748620371955937 1102 9 0.1560784931336059 1102 10-11 0.19057688808963036 1102 12-13 0.1631742559176459 1102 14-15 0.224592015299514 1102 16-17 0.25563983014369995 1102 18-19 0.25429607199397 1102 20-21 0.19858005715244786 1102 22-23 0.16645974491929394 1102 24-25 0.07985181713888068 1102 26-27 0.07511630395834601 1102 28-29 0.14903858722760788 1102 30-31 0.20207694259479325 1102 32-33 0.2639055481899888 1102 34-35 0.24899645067726794 1102 36-37 0.30405145309809 1102 38-39 0.2718960322114867 1102 40-41 0.2660549080195622 1102 42-43 0.32800702442757057 1102 44-45 0.1450186956955335 1102 46-47 0.1425533403372441 1102 48-49 0.1364702534807094 1102 50-51 0.23040335320726513 1102 52-53 0.20504790996277933 1102 54-55 0.2729346875663978 1102 56-57 0.2740314927997929 1102 58-59 0.21962131921959838 1102 60-61 0.1666961586712148 1102 62-63 0.08868931307240757 1102 64-65 0.08874608008285634 1102 66-67 0.06571440431662978 1102 68-69 0.11046729286773171 1102 70-71 0.06181055916724887 1102 72-73 0.11177244310849233 1102 74-75 0.13545620609562548 1102 76-77 0.013554648594279683 1102 78-79 0.13138157788768723 1102 80-81 0.11695404310046698 1102 82-83 0.15147862726106354 1102 84-85 0.20500266003960732 1102 86-87 0.08820349264941285 1102 88-89 0.3111619496158866 1102 90-91 0.31735609801130593 1102 92-93 0.32464895340165967 1102 94-95 0.3151512440991979 1102 96-97 0.36581570658106344 1102 98-99 0.4584343097580401 1102 100-101 0.838420240518829 1103 1 0.08719600354461221 1103 2 0.10546460974153149 1103 3 0.028971321940176153 1103 4 0.10803648060302606 1103 5 0.12450605301971507 1103 6 0.08853965823512766 1103 7 0.11809382787244971 1103 8 0.16608423914576775 1103 9 0.09992693928090546 1103 10-11 0.07220154093076303 1103 12-13 0.11392381500053261 1103 14-15 0.14612017317825376 1103 16-17 0.063316095626476 1103 18-19 0.08876616058282849 1103 20-21 0.18154241195388465 1103 22-23 0.051174618523049276 1103 24-25 0.06542191789096563 1103 26-27 0.06299640491315728 1103 28-29 0.18344938006751477 1103 30-31 0.10185825784545699 1103 32-33 0.06708952718813777 1103 34-35 0.008471607278856652 1103 36-37 -0.013671387421688053 1103 38-39 0.05812092020840254 1103 40-41 0.058120934089409104 1103 42-43 0.10647886654884786 1103 44-45 0.06057141316396297 1103 46-47 0.027655156713002782 1103 48-49 0.0979254826562439 1103 50-51 0.08641021529650317 1103 52-53 0.06157373655565124 1103 54-55 -0.01339054708260079 1103 56-57 -0.04025979629992804 1103 58-59 -0.10436837244250796 1103 60-61 -0.07760916882376279 1103 62-63 -0.00950647439541541 1103 64-65 -5.279777735864855E-4 1103 66-67 -0.11788221227806872 1103 68-69 -0.02326970767962422 1103 70-71 -0.0747673159163682 1103 72-73 -0.059674373165904626 1103 74-75 0.02033579011313691 1103 76-77 0.04186330070561084 1103 78-79 -0.06656917116459482 1103 80-81 -0.0035635530397364334 1103 82-83 -0.023854847976625848 1103 84-85 -0.11517943365781136 1103 86-87 0.02069224166114836 1103 88-89 0.24058835193500983 1103 90-91 0.2668840126254288 1103 92-93 0.257746117341739 1103 94-95 0.13219547771431195 1103 96-97 0.24769553386319032 1103 98-99 0.370403688825121 1103 100-101 0.8337546279988111 1104 1 -0.034975189871023105 1104 2 -0.01929176885928996 1104 3 -0.057378472298509564 1104 4 -0.039674630508081066 1104 5 0.018776834912721085 1104 6 0.04711578992237264 1104 7 -0.028937447847717124 1104 8 -0.056996419290449296 1104 9 -0.043475529854895 1104 10-11 -0.023207512567182675 1104 12-13 -0.06216383932044778 1104 14-15 -0.037978592253843146 1104 16-17 0.005452309618242168 1104 18-19 -0.03685647316201823 1104 20-21 -0.04664030409550435 1104 22-23 -0.09776406460451881 1104 24-25 -0.10503898745881912 1104 26-27 -0.03779124940782452 1104 28-29 0.006667487063396038 1104 30-31 -0.095555733924094 1104 32-33 -0.08520594606289222 1104 34-35 0.00579136036528638 1104 36-37 -0.11996480305954549 1104 38-39 0.05208306012609398 1104 40-41 0.08068101639393888 1104 42-43 0.12461096531427529 1104 44-45 0.07393478764955574 1104 46-47 0.05525104148667026 1104 48-49 0.019902437388758187 1104 50-51 -0.01930912626728798 1104 52-53 -0.11257852681882952 1104 54-55 -0.025445279592887005 1104 56-57 0.028070244852337112 1104 58-59 0.04897977570563938 1104 60-61 0.01565379413919743 1104 62-63 -0.08328342912792408 1104 64-65 0.09970617860501818 1104 66-67 -0.022155874829508093 1104 68-69 0.014285847875932234 1104 70-71 -0.04591669863241776 1104 72-73 -0.06866696575849573 1104 74-75 -0.08904198766464333 1104 76-77 -0.13054945649603056 1104 78-79 -0.1103983892715874 1104 80-81 -0.04191952011792566 1104 82-83 -0.13883056814123407 1104 84-85 -0.09354939250555105 1104 86-87 0.1850934762290528 1104 88-89 0.32196901037122316 1104 90-91 0.25601734595876025 1104 92-93 0.2910642243376209 1104 94-95 0.2622032966443548 1104 96-97 0.28428565731998034 1104 98-99 0.3093024542572209 1104 100-101 0.6962933111263823 1105 1 2.2481012897657138E-4 1105 2 -8.917688592902095E-4 1105 3 -0.043378472298506665 1105 4 -0.07947463050808068 1105 5 -0.017023165087280745 1105 6 -0.06228421007762819 1105 7 -0.05293744784771803 1105 8 -0.021596419290446534 1105 9 -0.011675529854898059 1105 10-11 0.011492487432818166 1105 12-13 -0.05496383932045035 1105 14-15 -0.022478592253847296 1105 16-17 -0.06054769038176033 1105 18-19 -0.14255647316202413 1105 20-21 0.024959695904499313 1105 22-23 -0.007864064604518717 1105 24-25 -0.05773898745882633 1105 26-27 0.021508750592168724 1105 28-29 0.016567487063397834 1105 30-31 -0.13485573392409123 1105 32-33 -0.019905946062891644 1105 34-35 -0.04710863963472178 1105 36-37 -0.08276480305954692 1105 38-39 -0.08531693987390554 1105 40-41 -0.0891189836060633 1105 42-43 -0.10348903468572246 1105 44-45 -0.045865212350442164 1105 46-47 -0.10994895851333553 1105 48-49 -0.07859756261124318 1105 50-51 3.9087373271939896E-4 1105 52-53 -0.012978526818834268 1105 54-55 -0.04854527959288646 1105 56-57 -0.12382975514766059 1105 58-59 -0.09912022429436007 1105 60-61 -0.027946205860800433 1105 62-63 -0.10858342912791841 1105 64-65 -0.08949382139498141 1105 66-67 -0.16135587482951053 1105 68-69 -0.14341415212405906 1105 70-71 -0.15521669863242238 1105 72-73 -0.2651669657584961 1105 74-75 -0.23414198766464267 1105 76-77 -0.08684945649603293 1105 78-79 -0.29399838927158584 1105 80-81 -0.163719520117926 1105 82-83 -0.17953056814123514 1105 84-85 -0.16174939250555198 1105 86-87 -0.0696065237709469 1105 88-89 0.11126901037122394 1105 90-91 0.15341734595876133 1105 92-93 0.107864224337618 1105 94-95 0.1187032966443553 1105 96-97 0.15828565731998268 1105 98-99 0.07720245425722183 1105 100-101 0.4264933111263858 1106 1 -0.076601120243172 1106 2 -0.08843566641831302 1106 3 -0.062472910073616106 1106 4 -0.061518007859021395 1106 5 -0.05409847521132605 1106 6 -0.11597424609203699 1106 7 -0.10025005288973432 1106 8 -0.11512775182345791 1106 9 -0.10934491761000231 1106 10-11 -0.16872804077846837 1106 12-13 -0.032554035398881354 1106 14-15 -0.021172669884897743 1106 16-17 -0.13421007533573714 1106 18-19 -0.2109417472716686 1106 20-21 -0.1789847618786098 1106 22-23 -0.19123333231159734 1106 24-25 -0.14471645844721337 1106 26-27 -0.12411769998806221 1106 28-29 -0.1405237894472009 1106 30-31 -0.1752420884659074 1106 32-33 -0.19407333301766982 1106 34-35 -0.1999865908151932 1106 36-37 -0.17776196192308902 1106 38-39 -0.13212678381147924 1106 40-41 -0.10717024411025733 1106 42-43 -0.10549999907147622 1106 44-45 -0.12937209510354108 1106 46-47 -0.06422851033406118 1106 48-49 -0.03786154820548404 1106 50-51 -0.07727575291794864 1106 52-53 -0.08111914306532952 1106 54-55 -0.09007517154967104 1106 56-57 -0.08745620572788937 1106 58-59 -0.13098012825594196 1106 60-61 -0.0915269581617224 1106 62-63 -0.0650859901523333 1106 64-65 -0.1293601279175931 1106 66-67 -0.09916447827088604 1106 68-69 -0.14051327177192263 1106 70-71 -0.2640559943507057 1106 72-73 -0.08356948676690479 1106 74-75 0.038336323659891036 1106 76-77 -0.02502888826873928 1106 78-79 -0.10297682064413749 1106 80-81 -0.14909979222677094 1106 82-83 0.08625598648061228 1106 84-85 -0.12720177345793005 1106 86-87 0.014493836373109303 1106 88-89 0.21624812201588028 1106 90-91 0.24854667769145422 1106 92-93 0.23537358808312092 1106 94-95 0.2480709637111822 1106 96-97 0.2626589266277044 1106 98-99 0.3651375082788313 1106 100-101 0.7782634191695976 1107 1 -0.04527932861757478 1107 2 -0.056441079068200395 1107 3 -0.1052511565712706 1107 4 -0.03328748033070639 1107 5 -0.03511760734190972 1107 6 -0.08225196727116213 1107 7 -0.07139807063052928 1107 8 -0.013639146921505585 1107 9 -0.07836666111229107 1107 10-11 -0.035322372638134425 1107 12-13 -0.058344879919580706 1107 14-15 -0.12052727179661815 1107 16-17 -0.10329696117403131 1107 18-19 -0.0030777187276456175 1107 20-21 -0.06153364268438111 1107 22-23 -0.07364151828997478 1107 24-25 -0.09323449396256933 1107 26-27 -0.08755372477242673 1107 28-29 -0.0970979839653765 1107 30-31 -0.19546137050272705 1107 32-33 -0.11518024641764413 1107 34-35 -0.1212891678176149 1107 36-37 -0.12002444042651206 1107 38-39 -0.06396155950338311 1107 40-41 -0.06455307899431517 1107 42-43 -0.20796684312087876 1107 44-45 -0.18281952847184613 1107 46-47 -0.2485771808231405 1107 48-49 -0.4837312244165233 1107 50-51 -0.3997710103823806 1107 52-53 -0.38103694227014984 1107 54-55 -0.39394204742891503 1107 56-57 -0.4229852537680756 1107 58-59 -0.4118469487720944 1107 60-61 -0.4002886969920567 1107 62-63 -0.4201339612524819 1107 64-65 -0.25912003345646895 1107 66-67 -0.322236087679336 1107 68-69 -0.22135033659390757 1107 70-71 -0.2991434625267786 1107 72-73 -0.13974233036236683 1107 74-75 -0.18079634320267246 1107 76-77 -0.2558936819591757 1107 78-79 -0.2371112390942116 1107 80-81 -0.27380383229766636 1107 82-83 -0.16195949995045922 1107 84-85 -0.23747919148071972 1107 86-87 -0.1477190976771361 1107 88-89 0.05699876992975916 1107 90-91 0.11077114966392188 1107 92-93 0.17678487865374137 1107 94-95 0.1385209552963076 1107 96-97 0.11890229113945239 1107 98-99 0.10042322682324212 1107 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6 274.0 7 713.0 8 1582.0 9 2919.0 10 4818.0 11 7331.0 12 10478.0 13 14670.0 14 17081.0 15 22295.0 16 28071.0 17 37579.0 18 36021.0 19 25609.0 20 24462.0 21 25746.0 22 27824.0 23 31761.0 24 37295.0 25 45013.0 26 55570.0 27 68439.0 28 85043.0 29 103948.0 30 127556.0 31 155324.0 32 190596.0 33 240716.0 34 316606.0 35 439133.0 36 666960.0 37 1093553.0 38 1034146.0 39 116450.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83004746239777 20.312243596575332 14.79462411959744 23.06308482142945 2 15.138537643921039 21.840870542841177 39.54314594301187 23.47744587022591 3 18.951646691574943 25.54269105529437 30.78296616228031 24.72269609085038 4 12.686422033363797 17.671740228107154 38.23521619824156 31.40662154028749 5 13.060674132607817 38.24534225787188 34.90853087162806 13.785452737892246 6 30.53848854430632 37.602239350613445 17.830244459956244 14.029027645123998 7 27.278132833100756 33.3849906284888 21.86349718383685 17.47337935457359 8 25.3588520502229 35.422035914935606 20.524266925286753 18.69484510955474 9 25.81992137389435 17.25213672613895 21.247887705923528 35.68005419404317 10-11 24.29209325472947 26.95893627705998 28.321156223317296 20.42781424489326 12-13 25.609167851801583 24.422299467224047 28.370000724131007 21.598531956843363 14-15 22.254819115858947 25.85873555756682 27.00474003031547 24.88170529625876 16-17 21.531521233051116 29.0978829502208 27.493485868590923 21.877109948137164 18-19 21.366548545530275 28.163222052854753 29.39376530378457 21.076464097830403 20-21 22.359904171348663 26.97246260716258 29.199018713982326 21.468614507506434 22-23 21.95811500652601 27.057027573692103 29.120879444354063 21.86397797542782 24-25 22.20283792633213 27.145287172892456 29.16079495847825 21.49107994229716 26-27 21.918532834709495 27.114059663541745 29.431212252307482 21.536195249441274 28-29 21.522213650831095 27.827501004265702 29.19081009065767 21.459475254245536 30-31 21.835630895706885 27.13845796988596 29.09332714256923 21.932583991837927 32-33 21.71647307773144 27.302898506072594 29.08173908401941 21.898889332176555 34-35 22.360596101483424 27.175787292552027 28.92383365774524 21.539782948219308 36-37 22.137385701811045 26.899169894896797 29.15262074974234 21.810823653549814 38-39 21.84449541847086 27.42263164996381 29.24592078309189 21.48695214847344 40-41 22.00562785762046 27.267093812869614 29.016133675945227 21.7111446535647 42-43 21.74123498966652 27.357021477270564 28.637770472525858 22.263973060537058 44-45 22.04597205347844 27.141424831832417 28.539163025169845 22.2734400895193 46-47 22.266364136251724 27.465261792033495 28.315853841857603 21.95252022985718 48-49 22.22648502671388 27.485790388221893 28.211168170548113 22.076556414516112 50-51 21.63387933478206 27.708822078662322 28.528143145352587 22.129155441203036 52-53 22.15182495732239 27.839040002449096 27.907979629350894 22.101155410877627 54-55 21.757889839068262 27.363880541540098 28.194658974524522 22.683570644867117 56-57 22.091569015277987 27.617954367187014 28.002528767527064 22.28794785000794 58-59 22.466958191163954 27.30659780393077 27.900162115774723 22.32628188913055 60-61 22.082136456472604 27.49168788951136 28.46887907565701 21.957296578359028 62-63 21.89419916114623 27.452915294529983 28.383668942217287 22.269216602106496 64-65 21.65599145800148 27.70674381834477 28.04398477715702 22.59327994649673 66-67 21.779453830128283 27.6482699937045 27.707977150949116 22.864299025218106 68-69 21.675250820466914 27.458616548592236 28.054714082476757 22.81141854846409 70-71 21.900890266530475 27.37255098206024 27.93758027656842 22.78897847484087 72-73 21.879412366705438 27.188342550467908 28.089208622928503 22.843036459898155 74-75 21.551453629220983 27.825715206927793 27.684823646625638 22.938007517225586 76-77 21.70996932919046 27.273183529457057 27.91061346726293 23.106233674089548 78-79 21.660720371391193 27.395715055257945 27.874834508850537 23.068730064500322 80-81 21.78499292304145 27.377963553050204 27.775990344918938 23.061053178989404 82-83 21.699733680706892 27.290162434948407 27.956362962720206 23.053740921624495 84-85 21.507210990778024 27.377097404255263 27.79688733561588 23.31880426935083 86-87 21.494548218184477 27.353236036730816 27.876569356306703 23.27564638877801 88-89 21.49161013719756 27.25600646015449 27.584901013174388 23.667482389473562 90-91 21.47003074244319 27.09082855036648 27.783114050882656 23.65602665630767 92-93 21.742161085592087 27.263934763057073 27.66447340663214 23.329430744718703 94-95 21.48213765499033 26.967058799749555 27.706241746204174 23.844561799055942 96-97 21.28071117914149 26.86614613612781 27.974979210667687 23.878163474063008 98-99 21.228443190065917 27.022653483525144 27.50087548438645 24.248027842022484 100-101 21.185957887477926 26.57506904311434 28.069271344688858 24.169701724718877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1541.0 1 1029.5 2 659.5 3 811.0 4 716.0 5 737.0 6 1288.5 7 2572.0 8 3903.5 9 4301.5 10 3891.0 11 3627.0 12 3792.0 13 4133.5 14 4512.5 15 4824.0 16 5179.0 17 5523.5 18 6008.0 19 6718.0 20 7685.5 21 9037.0 22 10792.0 23 13296.0 24 16224.0 25 20488.0 26 27729.5 27 36223.0 28 43793.0 29 52327.5 30 62789.5 31 73756.5 32 84799.5 33 95882.0 34 108181.0 35 119432.5 36 132208.5 37 145530.5 38 152814.0 39 155401.5 40 157116.5 41 162437.5 42 168332.5 43 175445.0 44 183418.5 45 187896.5 46 189923.5 47 204149.0 48 236146.0 49 238336.5 50 210098.0 51 191588.0 52 175233.5 53 164317.5 54 157529.5 55 143015.0 56 127550.0 57 111913.5 58 98148.5 59 86234.5 60 71851.5 61 56275.5 62 43291.0 63 33378.5 64 25091.5 65 18786.0 66 13585.5 67 9999.0 68 7094.5 69 4545.5 70 3036.0 71 2001.5 72 1382.0 73 1055.0 74 794.0 75 571.0 76 367.0 77 198.0 78 104.5 79 54.5 80 28.5 81 17.0 82 11.5 83 10.0 84 6.5 85 3.5 86 3.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3833380791061123 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.9436219855593753E-4 16-17 0.00216846819602874 18-19 1.0793280613717711E-4 20-21 1.9624146570395835E-5 22-23 0.0 24-25 0.0 26-27 8.830865956678127E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00763379301588398 36-37 0.016994510929962795 38-39 0.011293696351262804 40-41 0.007771162041876751 42-43 0.0037285878483752093 44-45 0.0020016629501803753 46-47 0.0015110592859204795 48-49 8.830865956678127E-5 50-51 3.826708581227188E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.3173122454870843E-4 60-61 6.966572032490522E-4 62-63 0.0 64-65 0.0 66-67 0.0011578246476533543 68-69 7.162813498194481E-4 70-71 0.001923166363898792 72-73 0.0 74-75 0.0 76-77 0.09672741844548108 78-79 0.03601030895667636 80-81 9.812073285197918E-6 82-83 0.0 84-85 0.0 86-87 0.14933975540071232 88-89 0.3263004970992568 90-91 0.27844701568734653 92-93 0.0 94-95 2.9436219855593757E-5 96-97 9.812073285197918E-6 98-99 2.453018321299479E-4 100-101 6.672209833934585E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5095763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.75437654510276 #Duplication Level Percentage of deduplicated Percentage of total 1 66.45477548157267 27.083229431962753 2 15.442569429423203 12.587045787012116 3 6.684583133696309 8.172780542331068 4 3.497723496398089 5.7019016169144425 5 2.0339514900935085 4.144621245087184 6 1.3327100779522354 3.258826100539119 7 0.9159411214167936 2.6130026548754586 8 0.6720465263815899 2.1911069753586916 9 0.4674221782803768 1.7144549513253566 >10 2.252033114404619 15.873969722561455 >50 0.14352027559279149 4.054411337727363 >100 0.09350434643801055 7.26187818517616 >500 0.005743981258682925 1.5388325586776475 >1k 0.0030891974142367743 2.1794571993424694 >5k 2.4134354798637154E-4 0.7215153725658061 >10k+ 1.4480612879182292E-4 0.9029663185428768 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19104 0.37489969608084206 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15057 0.2954807749104501 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11741 0.23040710488301752 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9279 0.18209245602670296 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8271 0.16231131628374398 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7066 0.13866421966641698 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6588 0.12928387760576776 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5474 0.1074225783263468 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.9624146570395835E-5 0.0 0.0 0.0 0.0 9 1.9624146570395835E-5 0.0 0.0 1.9624146570395835E-5 0.0 10-11 1.9624146570395835E-5 0.0 0.0 3.924829314079167E-5 0.0 12-13 7.849658628158334E-5 0.0 0.0 5.887243971118751E-5 0.0 14-15 1.668052458483646E-4 0.0 0.0 5.887243971118751E-5 0.0 16-17 1.7661731913356252E-4 0.0 0.0 8.830865956678126E-5 0.0 18-19 2.256776855595521E-4 0.0 0.0 1.668052458483646E-4 0.0 20-21 2.747380519855417E-4 0.0 0.0 3.7285878483752093E-4 0.0 22-23 3.1398634512633337E-4 0.0 0.0 7.16281349819448E-4 0.0 24-25 4.906036642598959E-4 0.0 0.0 0.0011185763545125628 0.0 26-27 4.906036642598959E-4 0.0 0.0 0.002178280269313938 0.0 28-29 5.985364703970731E-4 0.0 0.0 0.006103109583393105 0.0 30-31 6.475968368230626E-4 0.0 0.0 0.018633127168590845 0.0 32-33 6.672209833934585E-4 0.0 0.0 0.044625309301080135 0.0 34-35 7.26093423104646E-4 0.0 0.0 0.08357924024331587 0.0 36-37 7.653417162454376E-4 0.0 0.0 0.14449259119782454 0.0 38-39 8.43838302527021E-4 0.0 0.0 0.2395127088916812 0.0 40-41 8.634624490974169E-4 0.0 0.0 0.3465428042866201 0.0 42-43 9.419590353790001E-4 0.0 0.0 0.45833175522487996 0.0 44-45 0.0010204556216605834 0.0 0.0 0.5962306331750515 0.0 46-47 0.0011382005010829586 0.0 0.0 0.7627218926782897 0.0 48-49 0.0011970729407941461 0.0 0.0 0.9384365010696141 0.0 50-51 0.0011970729407941461 0.0 0.0 1.1306550167266414 0.0 52-53 0.001216697087364542 0.0 0.0 1.3268768582840291 0.0 54-55 0.0012559453805053335 0.0 0.0 1.539239560395568 0.0 56-57 0.0013344419667869168 0.0 0.0 1.7975227654818327 0.0 58-59 0.0013344419667869168 0.0 0.0 2.0711422411128617 0.0 60-61 0.0013344419667869168 0.0 0.0 2.371882287304178 0.0 62-63 0.001344254040072115 0.0 0.0 2.6777442357503674 0.0 64-65 0.0013933144064981044 0.0 0.0 2.997892562899805 0.0 66-67 0.0014914351393500835 0.0 0.0 3.3612140125041137 0.0 68-69 0.0015110592859204795 0.0 0.0 3.769523818121055 0.0 70-71 0.0015797437989168647 0.0 0.0 4.211479223032939 0.0 72-73 0.0016582403851984483 0.0 0.0 4.657585134944462 0.0 74-75 0.001687676605054042 0.0 0.0 5.127740438478006 0.0 76-77 0.001687676605054042 0.0 0.0 5.617441391995664 0.0 78-79 0.001687676605054042 0.0 0.0 6.177750024873605 0.0 80-81 0.001923166363898792 1.9624146570395835E-5 0.0 6.778239097854433 0.0 82-83 0.0019722267303247817 1.9624146570395835E-5 0.0 7.3840365024825525 0.0 84-85 0.002011475023465573 1.9624146570395835E-5 0.0 8.001529898466629 0.0 86-87 0.002060535389891563 1.9624146570395835E-5 0.0 8.638814246266946 0.0 88-89 0.002080159536461959 1.9624146570395835E-5 0.0 9.320547286049214 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 340 0.0 23.817368 1 GTACATG 19265 0.0 23.61338 1 TACATGG 19815 0.0 22.317978 2 ACATGGG 19720 0.0 21.414902 3 GAGTACT 14870 0.0 20.31149 12-13 AGTACTT 16025 0.0 19.173264 12-13 AGAGTAC 26215 0.0 18.571524 10-11 GTACTTT 16525 0.0 18.30598 14-15 CATGGGG 9400 0.0 17.7179 4 CATGGGA 11110 0.0 16.656492 4 GTATCAA 41535 0.0 16.572094 1 GAGTACA 16920 0.0 16.300539 1 AGTACAT 15280 0.0 16.116734 2 GTGTACG 490 2.910383E-11 15.554201 1 ACTTTTT 19635 0.0 15.502927 16-17 TACTTTT 19740 0.0 15.444729 14-15 GTATAGG 1390 0.0 15.078623 1 CAACGCA 45940 0.0 14.904188 5 TCAACGC 46055 0.0 14.897881 4 ATCAACG 46025 0.0 14.897283 3 >>END_MODULE