FastQCFastQC Report
Thu 26 May 2016
SRR938772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5112160
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT174160.3406779130543645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155860.30488091139557444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108490.21221949234765733No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT100400.19639447904603924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77470.15154064035554443No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT74530.14578964664642735No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC68150.13330959907358142No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT52900.10347876435792307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG186100.025.8675271
TACATGG192450.024.1834452
ACATGGG192850.023.3460623
GAGTACT140050.020.94606812-13
CATGGGG89600.020.756074
AGTACTT151900.020.01513312-13
AGAGTAC245200.019.87965210-11
GTACTTT159000.018.50964414-15
GTATCAA397400.018.3318421
GAGTACA161150.017.929371
CATGGGA110550.017.7238644
AGTACAT143950.017.4345632
CAACGCA437900.016.4353035
TCAACGC440350.016.3546354
ATCAACG442750.016.265983
AACGCAG444550.016.1776646
TATCAAC446000.016.1687262
TACTTTT190350.015.86016814-15
GTATATA30250.015.5525511
GTGTACG4301.198714E-915.4721761
Produced by FastQC (version 0.11.5)