##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938772_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5112160 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49615074645551 33.0 31.0 34.0 30.0 34.0 2 31.56689422866264 33.0 31.0 34.0 28.0 34.0 3 31.591404220525177 33.0 31.0 34.0 28.0 34.0 4 35.142844903132925 37.0 35.0 37.0 32.0 37.0 5 35.17809301743294 37.0 35.0 37.0 33.0 37.0 6 35.23160249287972 37.0 35.0 37.0 32.0 37.0 7 35.13670385903415 37.0 35.0 37.0 32.0 37.0 8 35.1040030046008 37.0 35.0 37.0 32.0 37.0 9 36.66222770805295 39.0 37.0 39.0 32.0 39.0 10-11 36.54560088103659 39.0 37.0 39.0 32.0 39.0 12-13 36.43584834981691 39.0 37.0 39.0 32.0 39.0 14-15 37.580447501643135 40.0 37.0 41.0 32.0 41.0 16-17 37.47669400018779 40.0 37.0 41.0 32.0 41.0 18-19 37.41994577634503 40.0 37.0 41.0 32.0 41.0 20-21 37.30813090748333 40.0 37.0 41.0 31.0 41.0 22-23 37.326985559919876 40.0 37.0 41.0 31.5 41.0 24-25 37.26294247441395 40.0 37.0 41.0 31.0 41.0 26-27 37.14210519623799 40.0 37.0 41.0 31.0 41.0 28-29 36.98513211636569 40.0 36.5 41.0 30.0 41.0 30-31 36.7818731221245 39.0 36.0 41.0 30.0 41.0 32-33 36.626858020875716 39.0 36.0 41.0 30.0 41.0 34-35 36.34156139479202 39.0 35.0 41.0 30.0 41.0 36-37 36.14120342477544 39.0 35.0 41.0 29.0 41.0 38-39 35.993488075490596 39.0 35.0 41.0 29.0 41.0 40-41 35.915697278645425 39.0 35.0 40.5 28.0 41.0 42-43 35.935701249569654 39.0 35.0 41.0 28.0 41.0 44-45 35.78638667412601 39.0 35.0 41.0 27.0 41.0 46-47 35.514339633344804 39.0 35.0 41.0 26.5 41.0 48-49 35.26042015508122 39.0 35.0 41.0 25.0 41.0 50-51 34.145141681794 38.0 33.5 40.0 23.5 40.5 52-53 34.24090814841476 38.0 33.5 39.5 24.0 40.5 54-55 34.76637624800476 38.0 34.0 40.0 24.0 41.0 56-57 34.671905026446744 38.0 34.0 40.0 23.5 41.0 58-59 34.48170919924259 38.0 34.0 40.0 23.0 41.0 60-61 34.17844394541642 38.0 34.0 40.0 23.0 41.0 62-63 33.8042048762167 37.5 33.0 40.0 22.0 41.0 64-65 33.42228099277018 37.0 33.0 40.0 21.0 41.0 66-67 33.03510981659416 36.5 32.5 39.0 20.0 41.0 68-69 32.61251682263466 36.0 32.0 39.0 18.5 41.0 70-71 32.18020523614284 35.5 31.5 39.0 17.5 40.0 72-73 31.753263004287817 35.0 31.0 38.0 13.5 40.0 74-75 31.284213424462457 35.0 31.0 37.0 9.5 39.5 76-77 30.816545648023535 35.0 30.5 37.0 7.5 39.0 78-79 30.37046532189916 34.5 30.0 36.0 4.5 39.0 80-81 29.875152088354042 34.0 29.5 36.0 2.0 37.5 82-83 29.444110121748928 34.0 29.0 35.0 2.0 37.0 84-85 29.074903758880787 34.0 29.0 35.0 2.0 37.0 86-87 28.645331816688053 34.0 29.0 35.0 2.0 36.0 88-89 28.181342720102656 34.0 28.0 35.0 2.0 36.0 90-91 27.71085157741542 34.0 27.0 35.0 2.0 35.5 92-93 27.220155765860223 33.5 26.0 35.0 2.0 35.0 94-95 26.687543132452817 33.0 25.0 35.0 2.0 35.0 96-97 26.071223709743045 33.0 24.5 35.0 2.0 35.0 98-99 25.284775906857377 32.5 19.0 35.0 2.0 35.0 100-101 22.77583418750587 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08107627579181909 1101 2 0.16298212956925795 1101 3 0.2626066813028203 1101 4 0.14513197278126455 1101 5 0.1122221700988888 1101 6 0.20515763312499047 1101 7 0.24010195232301612 1101 8 0.26722012973584697 1101 9 0.2994812434005141 1101 10-11 0.27052847620767295 1101 12-13 0.2813888659059032 1101 14-15 0.3207029942472346 1101 16-17 0.4256947679883112 1101 18-19 0.5039558240847484 1101 20-21 0.5824718093608467 1101 22-23 0.4317750884768614 1101 24-25 0.43742650629407365 1101 26-27 0.40266772044910226 1101 28-29 0.4997459226334513 1101 30-31 0.5502641871759906 1101 32-33 0.44195701306217927 1101 34-35 0.42585491822465116 1101 36-37 0.4239994567210701 1101 38-39 0.41623646431835937 1101 40-41 0.5770833083489961 1101 42-43 0.5770682163994465 1101 44-45 0.6080541017036225 1101 46-47 0.48503771848219657 1101 48-49 0.41373780694159024 1101 50-51 0.4852130371716328 1101 52-53 0.5384214443747979 1101 54-55 0.6154201531299464 1101 56-57 0.6708508110556224 1101 58-59 0.5700529009337671 1101 60-61 0.467927375850401 1101 62-63 0.3360956836534186 1101 64-65 0.31329914360682665 1101 66-67 0.33139563578098574 1101 68-69 0.5357705574933789 1101 70-71 0.38308903819241635 1101 72-73 0.3187277842241798 1101 74-75 0.28473626252416295 1101 76-77 0.24326133098010772 1101 78-79 0.15323408437402364 1101 80-81 0.11493270145483336 1101 82-83 0.23469582164862146 1101 84-85 0.2826943805543358 1101 86-87 0.07759202118728581 1101 88-89 0.08255671369093776 1101 90-91 0.12229484861274642 1101 92-93 0.10294973494285387 1101 94-95 -0.052699631078159115 1101 96-97 -0.16360906583487278 1101 98-99 -0.12025886822158682 1101 100-101 0.3069017062329422 1102 1 0.13624697607575698 1102 2 0.1346570205495219 1102 3 0.14956260883148786 1102 4 0.3151221106670903 1102 5 0.3054284823334683 1102 6 0.37214556016059674 1102 7 0.326614136877204 1102 8 0.30991287612638985 1102 9 0.41605345531888105 1102 10-11 0.474466909779089 1102 12-13 0.4518996558484858 1102 14-15 0.5056464654498001 1102 16-17 0.5950478689928431 1102 18-19 0.6780292014563898 1102 20-21 0.5227507653875705 1102 22-23 0.5869382090009765 1102 24-25 0.424622891571218 1102 26-27 0.47354575757940864 1102 28-29 0.5449215720310789 1102 30-31 0.6878158019697906 1102 32-33 0.651831975514483 1102 34-35 0.661888819533047 1102 36-37 0.6352199770127953 1102 38-39 0.558935755223608 1102 40-41 0.6266055427257626 1102 42-43 0.6939579034881831 1102 44-45 0.65069492533501 1102 46-47 0.662454676776342 1102 48-49 0.6428938486361275 1102 50-51 0.6888509888404499 1102 52-53 0.6631292785400831 1102 54-55 0.5599744708765257 1102 56-57 0.5457729770741082 1102 58-59 0.43356389704260323 1102 60-61 0.46346035334296687 1102 62-63 0.39704166737181623 1102 64-65 0.4171504199717688 1102 66-67 0.3800884903506372 1102 68-69 0.5098883661410767 1102 70-71 0.4444167183918779 1102 72-73 0.2368169030997791 1102 74-75 0.1272503809804384 1102 76-77 0.1427864182998242 1102 78-79 0.17684124432188142 1102 80-81 0.2508456395872187 1102 82-83 0.20184520679728735 1102 84-85 0.05724816783810738 1102 86-87 -0.04460720940890894 1102 88-89 0.10037053360339243 1102 90-91 0.018456813755161505 1102 92-93 -0.07987946879234542 1102 94-95 0.018065853239601637 1102 96-97 -0.05438344282797303 1102 98-99 -0.21140698475359088 1102 100-101 0.006328236612127114 1103 1 0.16567812575483032 1103 2 0.3778924914236619 1103 3 0.45044855345495804 1103 4 0.35645119515739054 1103 5 0.3281087240611811 1103 6 0.2716832760503536 1103 7 0.32490999612998905 1103 8 0.3973264461126149 1103 9 0.4703098643695327 1103 10-11 0.4198432465240529 1103 12-13 0.46845732122253736 1103 14-15 0.5511723949128609 1103 16-17 0.561103628749926 1103 18-19 0.5459557548336775 1103 20-21 0.5081093209829888 1103 22-23 0.5849070038373441 1103 24-25 0.5271868008525971 1103 26-27 0.48393962179783756 1103 28-29 0.6040258303168216 1103 30-31 0.6537453486074938 1103 32-33 0.6427882577349067 1103 34-35 0.6026400303334114 1103 36-37 0.6099050779163093 1103 38-39 0.6872292526916652 1103 40-41 0.7352931219604741 1103 42-43 0.8137655947668634 1103 44-45 0.8490010644247192 1103 46-47 0.7300867471960331 1103 48-49 0.7435480790175077 1103 50-51 0.822810035641794 1103 52-53 0.6675015095603598 1103 54-55 0.6575716605440931 1103 56-57 0.6641961516385138 1103 58-59 0.6810884658333194 1103 60-61 0.592895978969274 1103 62-63 0.495990253306438 1103 64-65 0.4375656800772276 1103 66-67 0.45478841734114184 1103 68-69 0.5039790612521671 1103 70-71 0.5068426489836781 1103 72-73 0.4753133964919556 1103 74-75 0.39257246424563164 1103 76-77 0.29418957112424593 1103 78-79 0.3921917982828411 1103 80-81 0.3723183754248218 1103 82-83 0.4285145564284818 1103 84-85 0.49341061488298266 1103 86-87 0.4481487277730061 1103 88-89 0.5541436458072262 1103 90-91 0.5795115851715416 1103 92-93 0.2883822338275195 1103 94-95 0.2824529087845882 1103 96-97 0.37746229290858935 1103 98-99 0.4781471737978187 1103 100-101 0.9062772631502369 1104 1 0.12317815168173851 1104 2 0.14818655230455136 1104 3 0.15005859330238636 1104 4 0.2538449522137327 1104 5 0.24221669598988882 1104 6 0.3007752892374569 1104 7 0.34523249892863817 1104 8 0.3740416724257969 1104 9 0.43651593316203474 1104 10-11 0.32170449183686145 1104 12-13 0.37584643629377723 1104 14-15 0.45889170423861003 1104 16-17 0.5657685499431935 1104 18-19 0.5442709329208739 1104 20-21 0.5737550150514394 1104 22-23 0.4620307139638484 1104 24-25 0.43931411219781324 1104 26-27 0.44334757701291494 1104 28-29 0.36128727817415296 1104 30-31 0.4523726090116753 1104 32-33 0.420863576749511 1104 34-35 0.5301120136363977 1104 36-37 0.5824428089508089 1104 38-39 0.5416855603321906 1104 40-41 0.5662726414103361 1104 42-43 0.5891203943390337 1104 44-45 0.6250663778659131 1104 46-47 0.5941358368779319 1104 48-49 0.7186202189462279 1104 50-51 0.7336769107258618 1104 52-53 0.7012334492338965 1104 54-55 0.6445292205417772 1104 56-57 0.6225929767708394 1104 58-59 0.6884550293477858 1104 60-61 0.6769325724630093 1104 62-63 0.6560709021169728 1104 64-65 0.7120091924476313 1104 66-67 0.5597532322903902 1104 68-69 0.5572213990927466 1104 70-71 0.7537759227492131 1104 72-73 0.5186887096686377 1104 74-75 0.4532845294566883 1104 76-77 0.43840401895038994 1104 78-79 0.23627042794056408 1104 80-81 0.24655639129799312 1104 82-83 0.2830251649239415 1104 84-85 0.3105395904056891 1104 86-87 0.40568742072501607 1104 88-89 0.4462492647765721 1104 90-91 0.5270173989170495 1104 92-93 0.49201919866452926 1104 94-95 0.5431799785881033 1104 96-97 0.5301337298937092 1104 98-99 0.6878710974533 1104 100-101 1.1432598858279306 1105 1 0.05629407662454611 1105 2 0.1462367696162623 1105 3 0.15246289306117333 1105 4 0.15835758104333308 1105 5 0.20100578334896113 1105 6 0.18945784830506085 1105 7 0.1413365505797941 1105 8 0.18160032770587975 1105 9 0.24783186131239177 1105 10-11 0.32378325909920846 1105 12-13 0.3097443444346979 1105 14-15 0.39625212023774026 1105 16-17 0.3618921653921703 1105 18-19 0.3299516319490152 1105 20-21 0.38333357558585845 1105 22-23 0.3960181947256345 1105 24-25 0.3621594982523888 1105 26-27 0.4229593129712583 1105 28-29 0.4717368030639122 1105 30-31 0.48492682547077237 1105 32-33 0.4476933918409145 1105 34-35 0.41962722841334 1105 36-37 0.6140524465780928 1105 38-39 0.6748118586375682 1105 40-41 0.6467619729415262 1105 42-43 0.5379395087154819 1105 44-45 0.6421616680067075 1105 46-47 0.6896219000037149 1105 48-49 0.6999226878861364 1105 50-51 0.7827429156811903 1105 52-53 0.7797726568477614 1105 54-55 0.7226963178963501 1105 56-57 0.6925706342312665 1105 58-59 0.7644975210067315 1105 60-61 0.7422491550906543 1105 62-63 0.6871543645770615 1105 64-65 0.626597575259737 1105 66-67 0.5495779531735749 1105 68-69 0.6195926064600172 1105 70-71 0.47461827163792236 1105 72-73 0.6101217680964908 1105 74-75 0.5725111475050397 1105 76-77 0.4722115696436582 1105 78-79 0.552645190231889 1105 80-81 0.6460446501188493 1105 82-83 0.5861568284871268 1105 84-85 0.4870666955357379 1105 86-87 0.4901483169585177 1105 88-89 0.5972892341842488 1105 90-91 0.68654743099599 1105 92-93 0.6956948836732799 1105 94-95 0.7292825959398961 1105 96-97 0.719689528176847 1105 98-99 0.676034487923161 1105 100-101 1.024199616932627 1106 1 0.08778814770962384 1106 2 0.12442341995489059 1106 3 0.21369939166028118 1106 4 0.21916193553756358 1106 5 0.15476070683536136 1106 6 0.12071616726156975 1106 7 0.2248365367833074 1106 8 0.16041369649254733 1106 9 0.09238433448332728 1106 10-11 0.19393395621627008 1106 12-13 0.25712806226678 1106 14-15 0.3908126340629252 1106 16-17 0.42298723169344754 1106 18-19 0.3439685946270927 1106 20-21 0.37216916548519663 1106 22-23 0.3445761473670217 1106 24-25 0.17504095484665783 1106 26-27 0.11718321427771627 1106 28-29 0.08292262692693697 1106 30-31 0.06650437263542841 1106 32-33 0.17172724317779142 1106 34-35 0.2080598950329957 1106 36-37 0.3516215513112755 1106 38-39 0.37195636426836387 1106 40-41 0.5674635296968589 1106 42-43 0.5233864172572851 1106 44-45 0.5299922600718716 1106 46-47 0.5612107537224986 1106 48-49 0.5789161519985413 1106 50-51 0.5962581349643941 1106 52-53 0.3595442686883388 1106 54-55 0.3234793856435516 1106 56-57 0.4963204369560188 1106 58-59 0.6486464387412383 1106 60-61 0.7052372352130973 1106 62-63 0.5022595931265457 1106 64-65 0.4517053703948193 1106 66-67 0.49678184212012866 1106 68-69 0.6375554754997665 1106 70-71 0.6257497409702211 1106 72-73 0.524910925571703 1106 74-75 0.46215760252913185 1106 76-77 0.5350404499217838 1106 78-79 0.5645685899153996 1106 80-81 0.5816959474928289 1106 82-83 0.5173942337128921 1106 84-85 0.7097487460945544 1106 86-87 0.7541509539135234 1106 88-89 0.8880788016123837 1106 90-91 0.9416150688481331 1106 92-93 0.9818589944849556 1106 94-95 1.0494769235521382 1106 96-97 1.1433184962561462 1106 98-99 1.3451463456508108 1106 100-101 1.7764230421506042 1107 1 0.02444490757386575 1107 2 0.04927225854908457 1107 3 0.10699790987909807 1107 4 0.14456619334782062 1107 5 0.07056934790102787 1107 6 -0.031296463190571444 1107 7 -0.0015220990771069864 1107 8 0.036261857106168804 1107 9 0.06426493896168495 1107 10-11 0.09709823082175717 1107 12-13 0.12448510021553005 1107 14-15 0.16832538693223142 1107 16-17 0.20226951817498673 1107 18-19 0.17355535955817203 1107 20-21 0.12977795454730057 1107 22-23 0.11405763497923971 1107 24-25 0.023863904445683204 1107 26-27 0.04593920067888746 1107 28-29 0.08978541386830585 1107 30-31 -0.0016737326583253775 1107 32-33 0.06354936570396319 1107 34-35 0.12012972991546178 1107 36-37 0.13941471446408826 1107 38-39 0.08768756097759933 1107 40-41 0.08087130919947327 1107 42-43 0.10024539902909879 1107 44-45 0.32870156645824267 1107 46-47 0.3415170763599278 1107 48-49 0.25971343378959233 1107 50-51 0.2946786219165105 1107 52-53 0.23637436202817952 1107 54-55 0.14328214982022303 1107 56-57 0.31656060097483163 1107 58-59 0.4216912174689682 1107 60-61 0.28820467484298007 1107 62-63 0.2384375202016713 1107 64-65 0.27219548747984135 1107 66-67 0.035950071564208486 1107 68-69 0.15167724698138585 1107 70-71 0.127428507085952 1107 72-73 0.1549502879527438 1107 74-75 0.14789565690657724 1107 76-77 0.07271599022269726 1107 78-79 0.1221697945925726 1107 80-81 0.012983510745304017 1107 82-83 -0.0921164263852603 1107 84-85 0.17947821858183488 1107 86-87 0.119612688672877 1107 88-89 0.24401315746306906 1107 90-91 0.31354930473288434 1107 92-93 0.4123254687639424 1107 94-95 0.43524858857840343 1107 96-97 0.604526674357114 1107 98-99 0.8122760394983182 1107 100-101 1.2832735729566132 1108 1 0.16950455013265753 1108 2 0.2798379751001647 1108 3 0.17857397199224678 1108 4 0.2542638816220091 1108 5 0.2673573857430114 1108 6 0.28534740736787967 1108 7 0.21476329920250237 1108 8 0.23330278785189051 1108 9 0.32236564267080325 1108 10-11 0.39082485675155 1108 12-13 0.38314199282819317 1108 14-15 0.49284097320740017 1108 16-17 0.6063141706775141 1108 18-19 0.5907780721408784 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2316 54-55 -2.4197108787148593 2316 56-57 -2.1656298709259616 2316 58-59 -1.9134516538879964 2316 60-61 -1.805031916953432 2316 62-63 -2.195510834986713 2316 64-65 -2.2292226559384396 2316 66-67 -1.9967782565877457 2316 68-69 -2.2045804162193043 2316 70-71 -2.031233472706255 2316 72-73 -2.0130470949248576 2316 74-75 -2.070557037705875 2316 76-77 -1.989999854144724 2316 78-79 -1.7801016014709035 2316 80-81 -1.7732420329367784 2316 82-83 -1.704466940492864 2316 84-85 -1.7180755754726817 2316 86-87 -1.7573453420051806 2316 88-89 -1.7567103555602905 2316 90-91 -2.145565426164673 2316 92-93 -1.938701703109519 2316 94-95 -1.8466349545007041 2316 96-97 -2.1309741030245597 2316 98-99 -2.443145592483429 2316 100-101 -2.350076409239854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23968.0 3 5681.0 4 5341.0 5 6102.0 6 7184.0 7 8546.0 8 10554.0 9 12345.0 10 14985.0 11 18532.0 12 22637.0 13 27617.0 14 29148.0 15 31205.0 16 36117.0 17 44172.0 18 40894.0 19 32774.0 20 34077.0 21 37027.0 22 40966.0 23 46392.0 24 52806.0 25 60715.0 26 70750.0 27 81546.0 28 95094.0 29 112564.0 30 133498.0 31 160662.0 32 196017.0 33 245099.0 34 318774.0 35 438001.0 36 642635.0 37 950456.0 38 876994.0 39 140244.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1442246393158 19.465000637511157 14.225306250551691 23.16546847262135 2 14.758451645264062 21.145160215841717 40.42221978392316 23.67416835497106 3 18.97191546809121 25.3382679676561 30.489842749199948 25.19997381505274 4 12.306592991911634 16.890503174647336 38.84760869880409 31.955295134636945 5 13.172765650398135 37.99687452818905 34.8639517806758 13.966408040737017 6 30.618956668819326 37.063569449738985 18.215284930172484 14.102188951269204 7 27.428678435778732 33.19122944278098 21.990729030018883 17.389363091421412 8 25.425655917217316 34.81397114654518 20.862650369924133 18.897722566313373 9 26.109121612355963 17.113768419732867 21.377508041797757 35.39960192611341 10-11 24.30385123631771 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28.888299494064967 21.613493197626813 36-37 22.201943963265418 26.782994151219835 29.118391644375315 21.896670241139432 38-39 21.89225380896357 27.30751554379956 29.224306794206644 21.57592385303023 40-41 22.036142650899443 27.176925862713674 29.02918519550852 21.757746290878362 42-43 21.716664416763823 27.25536233662637 28.65631711357919 22.37165613303062 44-45 21.989942616200135 27.105774551692747 28.589825814187396 22.31445701791972 46-47 22.273234923126807 27.341441731062332 28.355701699519276 22.029621646291588 48-49 22.237194872773447 27.397508354964888 28.245718120391437 22.119578651870224 50-51 21.700653784116213 27.574654879075716 28.553813822513447 22.170877514294624 52-53 22.149294941172347 27.77762774634046 27.950169354940872 22.12290795754632 54-55 21.770768216470106 27.24643726676993 28.2894559829153 22.693338533844663 56-57 22.082637591873215 27.527318488056284 28.11754352343896 22.272500396631536 58-59 22.494661557046843 27.16035665785007 28.040419443200104 22.30456234190298 60-61 22.132927671413846 27.259864804216903 28.635184695681932 21.972022828687315 62-63 21.91170406211585 27.26628989753398 28.582029761881383 22.239976278468784 64-65 21.715084141273422 27.513031545218325 28.267649159530155 22.504235153978097 66-67 21.841528147603405 27.441964810596836 27.90699478992371 22.809512251876047 68-69 21.69957921161163 27.219855695456 28.312659976303518 22.767905116628853 70-71 21.963695807180745 27.12562486021038 28.164063432391234 22.74661590021764 72-73 21.915612828858393 26.85971753473358 28.392342161035884 22.832327475372146 74-75 21.59763586749418 27.48387520045508 28.036418200575298 22.882070731475434 76-77 21.74275224734366 26.95301118960763 28.1811292755208 23.123107287527912 78-79 21.71396729567059 27.047760997096816 28.18421700576435 23.054054701468242 80-81 21.85626461349102 27.01765096655473 28.040671430695014 23.08541298925924 82-83 21.757236061142823 26.86003547343425 28.314695689564346 23.068032775858583 84-85 21.607951085771248 26.934316797342305 28.144662533397184 23.313069583489256 86-87 21.587322378573994 26.864015460552253 28.29172267978062 23.256939481093138 88-89 21.602974374575233 26.72723446580696 27.99909343715075 23.670697722467057 90-91 21.618750184773923 26.566546456804748 28.168748755691897 23.64595460272944 92-93 21.9398341573476 26.613486987816483 28.107663951469426 23.339014903366493 94-95 21.68360102756394 26.31879726337873 28.171579171841145 23.826022537216186 96-97 21.509150254058305 26.120085806868538 28.473448895672206 23.89731504340095 98-99 21.49236617002915 26.279856743031488 28.00368848779662 24.224088599142746 100-101 21.646054294739507 25.57404467292671 28.487416148902422 24.29248488343136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4984.0 1 3314.0 2 1560.5 3 1489.0 4 1411.5 5 1514.5 6 2079.5 7 3344.5 8 4877.0 9 5352.0 10 4815.0 11 4485.0 12 4676.0 13 4976.5 14 5291.5 15 5568.5 16 5836.0 17 6184.5 18 6621.5 19 7174.5 20 8037.0 21 9506.5 22 11322.0 23 13541.0 24 16481.0 25 20895.0 26 27897.5 27 36231.5 28 43571.5 29 51979.0 30 62256.5 31 73514.0 32 84680.0 33 95170.5 34 107406.0 35 118476.0 36 131430.5 37 144994.0 38 151569.0 39 153925.0 40 155803.5 41 161481.5 42 166936.5 43 173118.0 44 181724.5 45 186095.0 46 188836.0 47 202417.0 48 231353.0 49 233570.5 50 207659.0 51 190802.5 52 176072.0 53 165463.0 54 158086.5 55 144185.5 56 129430.5 57 114266.0 58 99781.5 59 87446.0 60 73694.5 61 58361.0 62 45385.0 63 35411.5 64 26681.5 65 19930.0 66 14628.5 67 11081.5 68 8078.5 69 5331.5 70 3649.0 71 2559.0 72 1820.5 73 1352.5 74 1046.5 75 805.5 76 546.0 77 360.0 78 252.5 79 174.0 80 124.0 81 95.5 82 71.5 83 55.0 84 42.5 85 32.5 86 21.5 87 17.5 88 16.5 89 13.5 90 11.5 91 7.5 92 6.5 93 3.5 94 2.5 95 3.0 96 3.5 97 2.5 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27796469594065915 2 0.16470533003661858 3 0.19545554129761197 4 0.1620841288222591 5 0.23727739350881036 6 0.19453616475227692 7 0.24197208225094674 8 0.24913148258270476 9 0.1482739194391412 10-11 0.20614573878751838 12-13 0.21135879941159902 14-15 0.17224617382867516 16-17 0.28724648680792464 18-19 0.23200564927545303 20-21 0.19861467559700793 22-23 0.24500406873024316 24-25 0.23326734687490217 26-27 0.3445999342743576 28-29 0.31588408813495666 30-31 0.29124675284028667 32-33 0.19097602578949016 34-35 0.3603760445682451 36-37 0.4010046633908172 38-39 0.3821183218052643 40-41 0.3447662045006416 42-43 0.3784603768270164 44-45 0.3869597195705925 46-47 0.3926520296704329 48-49 0.41128407561578667 50-51 0.40388994084692187 52-53 0.4036356452067228 54-55 0.3981291665362587 56-57 0.3769835059935526 58-59 0.3516713091922005 60-61 0.31867155957560017 62-63 0.3861381490407186 64-65 0.41931394948514916 66-67 0.42517252981127357 68-69 0.42942709148383457 70-71 0.4288109135864292 72-73 0.42019420362429977 74-75 0.41555819849144005 76-77 0.3995669149635379 78-79 0.3742840599668242 80-81 0.3851796500891991 82-83 0.3947548590028481 84-85 0.39565467434509094 86-87 0.39072525116584766 88-89 0.3990583236831398 90-91 0.42016486181966134 92-93 0.419216143469688 94-95 0.4277741698225408 96-97 0.42822407749366215 98-99 0.4250062595849895 100-101 0.39897029826922475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5112160.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.96953740562381 #Duplication Level Percentage of deduplicated Percentage of total 1 68.85469526764993 29.586544038561126 2 14.511833325044837 12.471335297693848 3 6.1771976797464285 7.962939802853905 4 3.216190445808121 5.527928626590479 5 1.8992563853227058 4.080508414599694 6 1.2032919148650352 3.10229381674067 7 0.8379163079839599 2.520341329708928 8 0.6102554101732546 2.0977914139539173 9 0.43904209932723437 1.6978892318716683 >10 2.024618960086819 15.069909100782553 >50 0.13304374198329963 3.9613090647834492 >100 0.0841536831169114 6.826673259998116 >500 0.005624130308271216 1.6005966381132088 >1k 0.002514851937754572 1.898630121222838 >5k 1.8289832274539326E-4 0.5364788320872806 >10k+ 1.8289832274539326E-4 1.0588310104382215 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17416 0.3406779130543645 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15586 0.30488091139557444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10849 0.21221949234765733 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10040 0.19639447904603924 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7747 0.15154064035554443 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7453 0.14578964664642735 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6815 0.13330959907358142 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5290 0.10347876435792307 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.912240618446997E-5 0.0 0.0 0.0 0.0 3 3.912240618446997E-5 0.0 0.0 0.0 0.0 4 3.912240618446997E-5 0.0 0.0 0.0 0.0 5 3.912240618446997E-5 0.0 0.0 0.0 0.0 6 5.8683609276704955E-5 0.0 0.0 0.0 0.0 7 7.824481236893994E-5 0.0 0.0 0.0 0.0 8 1.1736721855340991E-4 0.0 0.0 0.0 0.0 9 1.1736721855340991E-4 0.0 0.0 1.9561203092234986E-5 0.0 10-11 1.369284216456449E-4 0.0 0.0 4.890300773058747E-5 0.0 12-13 1.369284216456449E-4 0.0 0.0 7.824481236893994E-5 0.0 14-15 2.1517323401458484E-4 0.0 0.0 8.802541391505743E-5 0.0 16-17 2.4451503865293735E-4 0.0 0.0 1.9561203092234987E-4 0.0 18-19 3.5210165566022973E-4 0.0 0.0 2.738568432912898E-4 0.0 20-21 4.107852649369347E-4 0.0 0.0 3.423210541141122E-4 0.0 22-23 4.890300773058746E-4 0.0 0.0 5.477136865825795E-4 0.0 24-25 5.672748896748146E-4 0.0 0.0 0.0010954273731651593 0.0 26-27 5.672748896748146E-4 0.0 0.0 0.001750727676755031 0.0 28-29 6.455197020437545E-4 0.0 0.0 0.005144596413257801 0.0 30-31 6.846421082282245E-4 0.0 0.0 0.016861757065506557 0.0 32-33 6.846421082282245E-4 0.0 0.0 0.04106874589214735 0.0 34-35 8.117899283277518E-4 0.0 0.0 0.07602461581797126 0.0 36-37 9.389377484272793E-4 0.0 0.0 0.13183272824011769 0.0 38-39 0.0010563049669806892 0.0 0.0 0.21976033613971394 0.0 40-41 0.0010660855685268068 0.0 0.0 0.32169376545335043 0.0 42-43 0.001154110982441864 0.0 0.0 0.4289185002034365 0.0 44-45 0.0012519169979030391 0.0 0.0 0.5592156740008137 0.0 46-47 0.0013301618102719791 0.0 0.0 0.7173875622046259 0.0 48-49 0.001427967825733154 0.0 0.0 0.8853596131576477 0.0 50-51 0.001427967825733154 0.0 0.0 1.0711519201276956 0.0 52-53 0.001427967825733154 0.0 0.0 1.2589003474069669 0.0 54-55 0.001486651435009859 0.0 0.0 1.4665522988325872 0.0 56-57 0.0015257738411943288 0.0 0.0 1.7139721761447215 0.0 58-59 0.0015257738411943288 0.0 0.0 1.981051062564552 0.0 60-61 0.0015257738411943288 0.0 0.0 2.2719554943507245 0.0 62-63 0.0015257738411943288 0.0 0.0 2.570928922412444 0.0 64-65 0.0015648962473787987 0.0 0.0 2.881658633532597 0.0 66-67 0.0016431410597477387 0.0 0.0 3.2376627492097274 0.0 68-69 0.0016822634659322086 0.0 0.0 3.6400464774185473 0.0 70-71 0.0017213858721166785 0.0 0.0 4.070520093267817 0.0 72-73 0.001809411286031736 0.0 0.0 4.504925510938625 0.0 74-75 0.0018583142937623235 0.0 0.0 4.9606819817846075 0.0 76-77 0.0018583142937623235 0.0 0.0 5.4430319082344845 0.0 78-79 0.0018583142937623235 0.0 0.0 5.992437638884542 0.0 80-81 0.002122390535507496 9.780601546117493E-6 0.0 6.586873259052925 0.0 82-83 0.0021712935432380833 1.9561203092234986E-5 0.0 7.182883556070232 0.0 84-85 0.0022299771525147883 1.9561203092234986E-5 0.0 7.790298034490313 0.0 86-87 0.0022886607617914933 1.9561203092234986E-5 0.0 8.417684501267566 0.0 88-89 0.0022886607617914933 1.9561203092234986E-5 1.9561203092234986E-5 9.08708843228694 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18610 0.0 25.867527 1 TACATGG 19245 0.0 24.183445 2 ACATGGG 19285 0.0 23.346062 3 GAGTACT 14005 0.0 20.946068 12-13 CATGGGG 8960 0.0 20.75607 4 AGTACTT 15190 0.0 20.015133 12-13 AGAGTAC 24520 0.0 19.879652 10-11 GTACTTT 15900 0.0 18.509644 14-15 GTATCAA 39740 0.0 18.331842 1 GAGTACA 16115 0.0 17.92937 1 CATGGGA 11055 0.0 17.723864 4 AGTACAT 14395 0.0 17.434563 2 CAACGCA 43790 0.0 16.435303 5 TCAACGC 44035 0.0 16.354635 4 ATCAACG 44275 0.0 16.26598 3 AACGCAG 44455 0.0 16.177664 6 TATCAAC 44600 0.0 16.168726 2 TACTTTT 19035 0.0 15.860168 14-15 GTATATA 3025 0.0 15.552551 1 GTGTACG 430 1.198714E-9 15.472176 1 >>END_MODULE