FastQCFastQC Report
Thu 26 May 2016
SRR938772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5112160
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT194740.38093486901818413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148840.2911489468248255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116900.22867046414822698No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT93850.18358189102062533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81780.1599715188882977No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT68820.13462019968076117No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC65500.12812588025413915No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT56700.11091202153297236No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG186000.024.3715671
TACATGG190700.023.2454722
ACATGGG192150.022.2055473
AGTACTT158850.020.29789412-13
GAGTACT148050.020.1600212-13
AGAGTAC256750.019.90462510-11
CATGGGG92300.019.4371364
GTACTTT162200.018.50369314-15
CATGGGA107200.018.1965584
CGTATAG3451.0641088E-917.9237041
GTATCAA422400.017.1506061
TACTTTT195900.016.16834414-15
GAGTACA160650.015.544691
AGTACAT147250.015.5035782
CAACGCA464150.015.4098045
ACTTTTT196300.015.38566316-17
TCAACGC465950.015.3502744
ATCAACG469050.015.2488233
AACGCAG470650.015.20326
TATCAAC475600.015.1186492
Produced by FastQC (version 0.11.5)