##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938772_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5112160 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.065112203060934 33.0 31.0 34.0 30.0 34.0 2 32.26346886638916 34.0 31.0 34.0 30.0 34.0 3 32.35588557478639 34.0 31.0 34.0 30.0 34.0 4 35.9698076742512 37.0 35.0 37.0 35.0 37.0 5 35.87316574598604 37.0 35.0 37.0 35.0 37.0 6 35.89515468999405 37.0 35.0 37.0 35.0 37.0 7 35.814124753528844 37.0 35.0 37.0 33.0 37.0 8 35.796788832900376 37.0 35.0 37.0 33.0 37.0 9 37.44570651153329 39.0 37.0 39.0 34.0 39.0 10-11 37.37324086100591 39.0 37.0 39.0 34.0 39.0 12-13 37.304038312572374 39.0 37.0 39.0 34.0 39.0 14-15 38.52368783449657 40.0 38.0 41.0 34.0 41.0 16-17 38.461909642890674 40.0 38.0 41.0 33.5 41.0 18-19 38.59775007042033 40.0 38.0 41.0 34.0 41.0 20-21 38.59928572266909 40.0 38.0 41.0 34.0 41.0 22-23 38.52745620246628 40.0 38.0 41.0 34.0 41.0 24-25 38.454011611530156 40.0 38.0 41.0 34.0 41.0 26-27 38.290234753998305 40.0 38.0 41.0 33.5 41.0 28-29 38.13542416512785 40.0 38.0 41.0 33.0 41.0 30-31 37.94034830678226 40.0 37.0 41.0 33.0 41.0 32-33 37.8182869863228 40.0 37.0 41.0 33.0 41.0 34-35 37.79771847907733 40.0 37.0 41.0 33.0 41.0 36-37 37.70036227348127 40.0 37.0 41.0 33.0 41.0 38-39 37.595292205251795 40.0 37.0 41.0 32.0 41.0 40-41 37.45646742277238 40.0 37.0 41.0 32.0 41.0 42-43 37.28109174986699 40.0 37.0 41.0 31.0 41.0 44-45 37.06724095098745 40.0 36.0 41.0 31.0 41.0 46-47 36.80191719351507 40.0 36.0 41.0 30.5 41.0 48-49 36.536938397859224 39.0 36.0 41.0 30.0 41.0 50-51 36.33316993208351 39.0 35.5 41.0 30.0 41.0 52-53 36.14155034271228 39.0 35.0 41.0 29.5 41.0 54-55 35.890409435541926 39.0 35.0 40.0 28.5 41.0 56-57 35.63210814997966 38.5 35.0 40.0 28.0 41.0 58-59 35.34051037134988 38.0 34.5 40.0 28.0 41.0 60-61 34.99042185690588 38.0 34.0 40.0 27.0 41.0 62-63 34.658117019029135 37.5 34.0 40.0 26.0 41.0 64-65 34.27293560843166 37.0 33.5 39.5 26.0 41.0 66-67 33.870617605865235 36.0 33.0 39.0 26.0 40.5 68-69 33.541774514099714 36.0 33.0 39.0 25.5 40.0 70-71 33.11842430596852 35.5 32.0 38.5 25.0 40.0 72-73 32.63847894040875 35.0 32.0 38.0 23.5 40.0 74-75 32.1503947450784 35.0 31.5 37.0 22.0 39.0 76-77 30.164833847140933 33.0 29.0 35.5 20.0 37.5 78-79 31.13534191026885 34.0 31.0 36.0 20.0 38.0 80-81 31.18205934477794 34.0 31.0 36.0 20.0 37.5 82-83 30.85723656927796 34.0 31.0 35.0 19.5 37.0 84-85 30.454355888704576 34.0 31.0 35.0 18.0 36.5 86-87 29.88508115943163 34.0 30.0 35.0 11.0 36.0 88-89 29.284361796187913 34.0 29.0 35.0 4.5 36.0 90-91 28.768981213420552 34.0 29.0 35.0 2.0 35.0 92-93 28.479283805671187 34.0 29.0 35.0 2.0 35.0 94-95 28.141587900222216 33.5 29.0 35.0 2.0 35.0 96-97 27.538626529686084 33.0 27.0 35.0 2.0 35.0 98-99 26.729135727363776 33.0 25.5 35.0 2.0 35.0 100-101 23.095900265249913 28.5 12.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16341796124095254 1101 2 0.1597741610671406 1101 3 0.0946101624040594 1101 4 0.11018770137144429 1101 5 -0.05390769444905885 1101 6 0.0899466604181356 1101 7 0.17201899191932313 1101 8 0.23093615332756912 1101 9 0.29835620508178096 1101 10-11 0.2982599065022029 1101 12-13 0.18994186354512266 1101 14-15 0.3286870687559045 1101 16-17 0.34898159400307094 1101 18-19 0.31708732966628617 1101 20-21 0.3007718150453158 1101 22-23 0.2409061573843303 1101 24-25 0.26060567891146036 1101 26-27 0.10555654854861984 1101 28-29 0.14180459232208875 1101 30-31 0.2291781106778501 1101 32-33 0.25816719018430234 1101 34-35 0.31335989749807425 1101 36-37 0.2933044681803594 1101 38-39 0.3144493671521147 1101 40-41 0.394829282042366 1101 42-43 0.39811478364900665 1101 44-45 0.3608460881670652 1101 46-47 0.4236456878424022 1101 48-49 0.36304169934508224 1101 50-51 0.38095122389761826 1101 52-53 0.317578531503635 1101 54-55 0.33025786966874904 1101 56-57 0.33169181208881326 1101 58-59 0.3257354172308169 1101 60-61 0.45809444676404354 1101 62-63 0.19547644870706193 1101 64-65 0.3021672193595677 1101 66-67 0.3008615667696617 1101 68-69 0.26787105558626223 1101 70-71 0.2570602235431423 1101 72-73 0.2501023183490432 1101 74-75 0.05355619424026514 1101 76-77 0.06907513196440362 1101 78-79 -0.12197178955397092 1101 80-81 0.12325397553096451 1101 82-83 0.17930484653874856 1101 84-85 0.3112784591867346 1101 86-87 0.3118638221013441 1101 88-89 0.3151832723914829 1101 90-91 0.25578511150521877 1101 92-93 0.2834592673223568 1101 94-95 0.27286357702505626 1101 96-97 0.16690506698191498 1101 98-99 0.09754616594518595 1101 100-101 0.8318780125769614 1102 1 0.2015106481008928 1102 2 0.12812021734900014 1102 3 0.17815047119761118 1102 4 0.09270851040025718 1102 5 -0.10685498514436631 1102 6 -0.08230584918857886 1102 7 0.05137369233744238 1102 8 0.1009616099248376 1102 9 0.22368176760308955 1102 10-11 0.18604409031615887 1102 12-13 0.19690330515845744 1102 14-15 0.2600426933509894 1102 16-17 0.28102224591217606 1102 18-19 0.27929833477131893 1102 20-21 0.24433542728679924 1102 22-23 0.17307579361283842 1102 24-25 0.23238385364912517 1102 26-27 0.17023029746893314 1102 28-29 0.21562820276432149 1102 30-31 0.1995941756918853 1102 32-33 0.19873844245451266 1102 34-35 0.2921305310769142 1102 36-37 0.2511354635316181 1102 38-39 0.24382925853483783 1102 40-41 0.27047764442502853 1102 42-43 0.3300060472796318 1102 44-45 0.22528250513715165 1102 46-47 0.2619935867000791 1102 48-49 0.22465743449311049 1102 50-51 0.25415269616063796 1102 52-53 0.11644043598389686 1102 54-55 0.1406736714576695 1102 56-57 0.08213726843408864 1102 58-59 0.09445465530324526 1102 60-61 0.03653386610548637 1102 62-63 0.04587369794040086 1102 64-65 0.13883232326085704 1102 66-67 0.20172426702438884 1102 68-69 0.12413208566157863 1102 70-71 0.13389418234375938 1102 72-73 0.14985863047528625 1102 74-75 0.07140396056249187 1102 76-77 -0.01735567750820266 1102 78-79 -0.11294946782338044 1102 80-81 0.07843797349835313 1102 82-83 0.11798697709246753 1102 84-85 0.14311928069948365 1102 86-87 0.0575297162397419 1102 88-89 -0.025623193187779947 1102 90-91 0.05672244169793572 1102 92-93 0.16142882581920048 1102 94-95 0.16412831111111004 1102 96-97 0.3049681650917755 1102 98-99 0.41606418526928124 1102 100-101 1.1034017617821732 1103 1 0.16634817687965153 1103 2 0.16315426872295546 1103 3 0.16927868290945014 1103 4 0.01193875371173192 1103 5 0.021959925024816584 1103 6 0.049493963173155464 1103 7 0.10623919734868537 1103 8 0.08902496949662009 1103 9 0.14762538644703938 1103 10-11 0.2029288396351845 1103 12-13 0.16752708933437788 1103 14-15 0.21158247973738753 1103 16-17 0.15110551454411336 1103 18-19 0.015479013685030907 1103 20-21 0.17743686931903824 1103 22-23 0.20029478643956367 1103 24-25 0.20931007226774057 1103 26-27 0.22629052106555037 1103 28-29 0.1019354912272874 1103 30-31 0.21417853809924736 1103 32-33 0.26968335826862955 1103 34-35 0.17707990073191837 1103 36-37 0.2124975325503371 1103 38-39 0.17688570675197468 1103 40-41 0.3020860338811815 1103 42-43 0.2600469139276953 1103 44-45 0.33646751451028223 1103 46-47 0.3430036033309989 1103 48-49 0.29962175873307473 1103 50-51 0.39897343349756653 1103 52-53 0.2946757012222392 1103 54-55 0.3231961403417287 1103 56-57 0.19714850966229847 1103 58-59 0.37990348364594695 1103 60-61 0.44998273616119633 1103 62-63 0.3748373845336417 1103 64-65 0.37238182089046035 1103 66-67 0.38369329635977323 1103 68-69 0.3452511630821462 1103 70-71 0.3703783546952195 1103 72-73 0.45614412584020414 1103 74-75 0.4385166023833236 1103 76-77 0.40505506610871933 1103 78-79 0.2729107727162088 1103 80-81 0.4205818600476974 1103 82-83 0.3779671774703921 1103 84-85 0.4229059967364428 1103 86-87 0.45523721496287095 1103 88-89 0.4144563521278464 1103 90-91 0.36384036169948075 1103 92-93 0.43250798880457353 1103 94-95 0.5581295614861084 1103 96-97 0.6261422193301804 1103 98-99 0.7665222609736411 1103 100-101 1.3130151942169874 1104 1 0.11583455410411858 1104 2 0.1931860984236664 1104 3 0.0969316107995084 1104 4 0.053879638724779966 1104 5 -0.01920695917542048 1104 6 0.08245330033916787 1104 7 0.07801028809399924 1104 8 0.023647711199615173 1104 9 0.11706810469657114 1104 10-11 0.13826003659111308 1104 12-13 0.09311619631875345 1104 14-15 0.08692183580459556 1104 16-17 0.21775146298758585 1104 18-19 0.176550594835426 1104 20-21 0.18344148349340372 1104 22-23 0.17538646380035772 1104 24-25 0.12534279198976606 1104 26-27 0.1330700915613221 1104 28-29 0.09895350811888903 1104 30-31 -0.05159276262490664 1104 32-33 0.010931938454696422 1104 34-35 0.08443034877806355 1104 36-37 0.18000423888071282 1104 38-39 0.13580895497940304 1104 40-41 0.17234198705908454 1104 42-43 0.11449512305014764 1104 44-45 0.13044439998026292 1104 46-47 0.08510961654474869 1104 48-49 0.22218359305478685 1104 50-51 0.10949054582160045 1104 52-53 0.15845753980801192 1104 54-55 0.08780651753030355 1104 56-57 0.012829831383946555 1104 58-59 0.1690135481773467 1104 60-61 0.2259590047455049 1104 62-63 0.10868652161032344 1104 64-65 0.06654280101856358 1104 66-67 0.0812743564080094 1104 68-69 0.10667319060848257 1104 70-71 0.08266659544113253 1104 72-73 0.10255834091842786 1104 74-75 0.1365417991452702 1104 76-77 0.07092287320209678 1104 78-79 0.024839200771324954 1104 80-81 0.24529240794215923 1104 82-83 0.2261284506988126 1104 84-85 0.30722297762755346 1104 86-87 0.30349262132572363 1104 88-89 0.3583803292218981 1104 90-91 0.41064010507938775 1104 92-93 0.46979888730155395 1104 94-95 0.4347894809413937 1104 96-97 0.3726310456474984 1104 98-99 0.634613501635922 1104 100-101 1.5292276427773182 1105 1 0.07878265464702139 1105 2 0.09278889645623423 1105 3 0.08116205231916496 1105 4 0.031622975118935415 1105 5 -0.11547005767835117 1105 6 -0.08993105685756575 1105 7 -0.0024158023232772052 1105 8 0.03352071826567027 1105 9 0.04597212595559341 1105 10-11 0.0472740450665583 1105 12-13 0.014018851751707473 1105 14-15 0.011240193846894897 1105 16-17 0.036111325172008435 1105 18-19 0.07795229545267546 1105 20-21 0.009666447377156828 1105 22-23 -0.010877213654495677 1105 24-25 -0.02762921619654435 1105 26-27 0.11830181280359398 1105 28-29 0.04564739121314432 1105 30-31 -0.012659553771925403 1105 32-33 0.019758282336894695 1105 34-35 0.039330676346558846 1105 36-37 0.060424619398851576 1105 38-39 0.14178125923876905 1105 40-41 0.11282584300174392 1105 42-43 0.1525187859185948 1105 44-45 0.13890284209456638 1105 46-47 0.0042090471586107014 1105 48-49 0.053863103013505054 1105 50-51 0.026642127378352143 1105 52-53 0.09138948543111525 1105 54-55 0.09644006700948893 1105 56-57 0.08395447336360462 1105 58-59 0.12083092831410625 1105 60-61 0.09542371535086858 1105 62-63 0.16249868117038346 1105 64-65 0.09344495666472596 1105 66-67 0.03735709944355392 1105 68-69 0.13707760729508323 1105 70-71 0.14429102307207842 1105 72-73 0.20255442815886227 1105 74-75 0.15635748087139945 1105 76-77 0.2579124016793095 1105 78-79 -0.01271379351911861 1105 80-81 0.1961336455616589 1105 82-83 0.2609168642442903 1105 84-85 0.22865021582554945 1105 86-87 0.24379608085351734 1105 88-89 0.15677380019110032 1105 90-91 0.17755673338622202 1105 92-93 0.3468091386178713 1105 94-95 0.4321313067828747 1105 96-97 0.4477612614896458 1105 98-99 0.5950874642115132 1105 100-101 1.365734917950892 1106 1 0.11517969989090915 1106 2 0.09008857977927676 1106 3 0.05729401073347162 1106 4 0.04934730718255764 1106 5 -0.032075844584291247 1106 6 0.008419247621532122 1106 7 0.09201348787857455 1106 8 0.0277365570186916 1106 9 0.0740186798472493 1106 10-11 0.044622545344019215 1106 12-13 0.09064847666083864 1106 14-15 0.13275681672246975 1106 16-17 0.1534447456641388 1106 18-19 0.02754478429174867 1106 20-21 0.0011379520722698544 1106 22-23 0.06687098561123861 1106 24-25 0.033177547209078284 1106 26-27 0.10884210900041325 1106 28-29 0.2556008269558703 1106 30-31 0.1775323225904799 1106 32-33 0.1761829512247246 1106 34-35 0.09010332007718347 1106 36-37 0.12268665727658146 1106 38-39 0.20896178320473524 1106 40-41 0.2725221055538114 1106 42-43 0.28948565550319927 1106 44-45 0.3353488729166614 1106 46-47 0.33008440906104397 1106 48-49 0.2330306328734224 1106 50-51 0.24959507097317868 1106 52-53 0.36680662128802766 1106 54-55 0.4002130828804198 1106 56-57 0.3640079606862372 1106 58-59 0.37942459197196854 1106 60-61 0.3663583598327733 1106 62-63 0.35073753261894325 1106 64-65 0.3751439312130387 1106 66-67 0.3207088426997373 1106 68-69 0.25897051308022156 1106 70-71 0.3971103253725019 1106 72-73 0.36418097696114415 1106 74-75 0.46960804838902703 1106 76-77 0.4541664495629192 1106 78-79 0.2964647647751484 1106 80-81 0.29353312351405947 1106 82-83 0.37007102683588045 1106 84-85 0.4722958908003356 1106 86-87 0.5217054910395902 1106 88-89 0.6126520513272631 1106 90-91 0.5832594405792477 1106 92-93 0.7497539707510583 1106 94-95 0.9253402570712623 1106 96-97 1.0703948162822314 1106 98-99 1.1378041060079944 1106 100-101 1.8078902213445254 1107 1 -0.03664901643607976 1107 2 -0.041001602018141625 1107 3 -0.03859878528319882 1107 4 -0.017476314917821867 1107 5 -0.102752290974081 1107 6 -0.12651042090623577 1107 7 -0.06344138632878327 1107 8 -0.03993389193941255 1107 9 -0.10136438205834963 1107 10-11 -0.12428852010057057 1107 12-13 -0.011102103198552982 1107 14-15 -0.081801251549102 1107 16-17 -0.015488020349515352 1107 18-19 -0.02774837381441131 1107 20-21 -0.02363739296677636 1107 22-23 -0.050452095633097827 1107 24-25 -0.13059664748352162 1107 26-27 -0.09432910834527775 1107 28-29 -0.11487694747991384 1107 30-31 -0.15364402704793179 1107 32-33 -0.05204561041541922 1107 34-35 -3.1981506531764126E-4 1107 36-37 -0.024889684748615082 1107 38-39 -0.057259356119836013 1107 40-41 -0.18671831396937932 1107 42-43 -0.07350531446465425 1107 44-45 -0.05398464132684211 1107 46-47 -0.11949987003739437 1107 48-49 -0.07142799055782945 1107 50-51 -0.16010031792264812 1107 52-53 -0.15106480666132427 1107 54-55 -0.07459381810240728 1107 56-57 -0.07654325828014663 1107 58-59 -0.04627161419179515 1107 60-61 -0.031290077106241654 1107 62-63 -0.029545726855552346 1107 64-65 -0.04794636824326659 1107 66-67 -0.03394298068156587 1107 68-69 -0.014027872570473221 1107 70-71 0.038894201546888496 1107 72-73 -0.08089383737281963 1107 74-75 0.0287129727311779 1107 76-77 -0.07354611436003822 1107 78-79 -0.05795822934969053 1107 80-81 0.12627263507390296 1107 82-83 0.02872410351516308 1107 84-85 0.03357134066538592 1107 86-87 0.12390706063237644 1107 88-89 0.062485778827525706 1107 90-91 0.29554489692995745 1107 92-93 0.4944267694265889 1107 94-95 0.5238586056593988 1107 96-97 0.6664858012169894 1107 98-99 0.7879599319547204 1107 100-101 1.4071960987237624 1108 1 0.18441806604865008 1108 2 0.1424021812596976 1108 3 0.08042384623339416 1108 4 0.03462874845437369 1108 5 0.02205482212921339 1108 6 0.0743680475384565 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38.0 5 107.0 6 295.0 7 680.0 8 1587.0 9 3021.0 10 5093.0 11 7803.0 12 10938.0 13 15031.0 14 17445.0 15 22673.0 16 28690.0 17 38912.0 18 37447.0 19 27030.0 20 26051.0 21 27471.0 22 30095.0 23 34077.0 24 39802.0 25 47930.0 26 58501.0 27 71855.0 28 88301.0 29 107752.0 30 130992.0 31 160274.0 32 196637.0 33 247651.0 34 326349.0 35 452716.0 36 688961.0 37 1098953.0 38 968032.0 39 92933.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.84318259847582 20.310902285640225 14.776966305683914 23.068948810200038 2 15.112829019436012 21.826038308660138 39.53567572219962 23.525456949704235 3 18.919087039529277 25.54519029138368 30.763004287815715 24.77271838127132 4 12.67951316077744 17.67137570029107 38.23514913461237 31.413962004319114 5 13.054989477968304 38.255857068643174 34.89984258531847 13.789310868070062 6 30.560806294392055 37.566077638425355 17.864353511624362 14.00876255555823 7 27.293316328127442 33.35683937904917 21.896732496635472 17.453111796187912 8 25.36818096460205 35.394275609527085 20.520797471127665 18.7167459547432 9 25.80050311414353 17.21790789020688 21.254049169040094 35.727539826609494 10-11 24.305665315639573 26.96018903946668 28.305158680479487 20.428986964414257 12-13 25.624579434133516 24.408889784357296 28.379442349222245 21.58708843228694 14-15 22.252677928703328 25.86655151638446 27.006998998466404 24.87377155644581 16-17 21.598908763479805 29.086752560006367 27.451276887964966 21.863061788548862 18-19 21.38303574223029 28.17113509749304 29.37533254045257 21.070496619824105 20-21 22.36779560890113 26.96873728521799 29.205952082876905 21.457515023003975 22-23 21.974400253513192 27.090652483490345 29.06748810678852 21.867459156207943 24-25 22.212163378529993 27.150652451746975 29.15431412732103 21.482870042402002 26-27 21.943363918644305 27.109260206359643 29.400503061905447 21.546872813090605 28-29 21.52291790554286 27.8032964539451 29.1889631779913 21.484822462520736 30-31 21.839261681950486 27.13720814684986 29.059516916528434 21.964013254671215 32-33 21.739255031141436 27.307009170292005 29.056191512002755 21.8975442865638 34-35 22.39102313614118 27.175240851694827 28.887668403006177 21.546067609157816 36-37 22.16260416624888 26.911304604038882 29.1177063571945 21.80838487251774 38-39 21.83553565761986 27.432739799405958 29.236926035006334 21.494798507967847 40-41 22.02401737968568 27.26792280086038 28.998887848794386 21.709171970659554 42-43 21.73029567792884 27.372282657868595 28.58825123209326 22.3091704321093 44-45 22.06845204909743 27.155710199496948 28.518328984706052 22.25750876669957 46-47 22.31389196463354 27.455123171225154 28.269284544030214 21.961700320111095 48-49 22.26116275034629 27.51017249571553 28.173015661564822 22.05564909237336 50-51 21.683394597409293 27.670796983309497 28.482645833457624 22.16316258582358 52-53 22.19190127069575 27.840423617414167 27.84580294826453 22.121872163625554 54-55 21.7756193076899 27.360499280147728 28.15105552251886 22.71282588964352 56-57 22.115966475156586 27.637759471934125 27.98262823841406 22.263645814495227 58-59 22.499916375513262 27.27034613104221 27.907388668422357 22.322348825022168 60-61 22.08376442456192 27.50438329062034 28.420018125882358 21.99183415893539 62-63 21.902558272279105 27.462470574841525 28.353343288755628 22.28162786412374 64-65 21.67863473104963 27.731203970519225 28.00374485752073 22.58641644091042 66-67 21.813232188163195 27.63908561276271 27.679129741509968 22.868552457564128 68-69 21.671570996854747 27.433061908015592 28.06786343668778 22.82750365844188 70-71 21.94636666017234 27.37563172954816 27.920930034216486 22.757071576063016 72-73 21.90600450690119 27.148671011861914 28.083432443429 22.861892037807895 74-75 21.574237320229358 27.818932544674546 27.670697138712384 22.936132996383705 76-77 21.716853754007705 27.270911427753 27.89159232904876 23.12064248919054 78-79 21.709451100961733 27.375390682007616 27.866756254585095 23.048401962445556 80-81 21.814009010969556 27.39364087476728 27.742450391008393 23.049899723254764 82-83 21.71964435114114 27.27878506788083 27.935616156190356 23.065954424787677 84-85 21.517460329880127 27.368763888454197 27.75188961221871 23.361886169446965 86-87 21.52652077879493 27.319526005571714 27.833920423319668 23.320032792313683 88-89 21.523180108030857 27.22532668880419 27.544737201150788 23.706756002014167 90-91 21.519343944342577 27.080176834006707 27.720395906761325 23.680083314889394 92-93 21.753773765017208 27.226241633634157 27.634740155582715 23.385244445765917 94-95 21.489133771890145 26.932375666464175 27.722327597614814 23.856162964030865 96-97 21.280595600385553 26.836572265792146 27.949166226946474 23.933665906875824 98-99 21.23861537980032 26.99745313135739 27.48370551782417 24.280225971018123 100-101 21.28165466165829 26.415198052073485 28.107173124952013 24.19597416131621 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1537.0 1 1017.5 2 637.0 3 821.0 4 733.0 5 744.0 6 1277.5 7 2516.5 8 3834.0 9 4232.5 10 3823.5 11 3511.0 12 3784.0 13 4145.0 14 4478.0 15 4793.0 16 5110.0 17 5515.0 18 5893.0 19 6542.5 20 7567.0 21 8963.0 22 10806.0 23 13292.5 24 16242.0 25 20689.0 26 27932.5 27 36372.0 28 43747.0 29 52299.5 30 62674.0 31 73470.5 32 85151.5 33 96359.5 34 108073.5 35 119446.0 36 132495.0 37 145953.5 38 153131.5 39 155127.5 40 156866.5 41 163000.5 42 169269.0 43 176207.0 44 184800.5 45 188821.0 46 190179.5 47 204732.5 48 236631.0 49 238351.5 50 210177.5 51 191630.5 52 175778.0 53 165077.5 54 158141.0 55 143774.0 56 128411.0 57 113210.0 58 99194.5 59 86879.0 60 72281.5 61 56977.5 62 44091.0 63 33847.5 64 25301.5 65 18950.0 66 13622.0 67 10112.5 68 7232.0 69 4583.0 70 3056.5 71 2026.5 72 1416.5 73 1124.5 74 863.0 75 617.0 76 404.0 77 222.5 78 121.0 79 71.0 80 45.0 81 28.0 82 22.0 83 15.5 84 10.0 85 5.0 86 3.0 87 2.5 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23671011861913557 2 0.0 3 0.0 4 0.0 5 0.0284615504992019 6 0.003540577759694532 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0031493536978498327 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.814434602985822E-4 26-27 0.0012812588025413914 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006895324090012833 36-37 0.01798652624331007 38-39 0.01451441269443836 40-41 0.008704735376044569 42-43 0.008460220337391631 44-45 0.003951363024631467 46-47 5.574942881286971E-4 48-49 0.0017800694813933835 50-51 2.9341804638352478E-5 52-53 0.0 54-55 0.0 56-57 0.0013790648180025664 58-59 4.107852649369347E-4 60-61 0.002601640011267253 62-63 7.433257175049295E-4 64-65 0.001623579856655504 66-67 0.005858580326124378 68-69 2.1517323401458482E-4 70-71 7.824481236893993E-5 72-73 0.0 74-75 4.107852649369347E-4 76-77 0.060512581765828924 78-79 0.14289458858877657 80-81 0.0011247691778035116 82-83 7.628869205971644E-4 84-85 0.0 86-87 0.10710736753153265 88-89 0.3568941504178273 90-91 0.3942169259178116 92-93 4.6946887421363964E-4 94-95 2.640762417451723E-4 96-97 1.467090231917624E-4 98-99 0.0 100-101 0.0011247691778035116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5112160.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.4105154787838 #Duplication Level Percentage of deduplicated Percentage of total 1 66.06738201881112 26.69816963713889 2 15.605632389128091 12.61263298434141 3 6.5779312737735935 7.9745278067161145 4 3.6214809159004115 5.853836424324548 5 2.0932581710749183 4.2294820861656826 6 1.4007674754982664 3.3963441450479763 7 0.930782679038734 2.6329385501075033 8 0.6776661988023829 2.190787233292182 9 0.507423295426627 1.8454713240719876 >10 2.2675729608343573 15.878005709113285 >50 0.14637430675728966 4.081644517443576 >100 0.0942908257672454 7.248270382334233 >500 0.006098071977153859 1.648297822866943 >1k 0.002952238403010558 2.0919220396510902 >5k 2.4198675434431515E-4 0.717080099321777 >10k+ 1.4519205260658908E-4 0.900589238062708 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19474 0.38093486901818413 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14884 0.2911489468248255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11690 0.22867046414822698 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9385 0.18358189102062533 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8178 0.1599715188882977 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6882 0.13462019968076117 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6550 0.12812588025413915 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5670 0.11091202153297236 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.8683609276704955E-5 0.0 0.0 0.0 0.0 3 5.8683609276704955E-5 0.0 0.0 0.0 0.0 4 5.8683609276704955E-5 0.0 0.0 0.0 0.0 5 9.780601546117492E-5 0.0 0.0 0.0 0.0 6 9.780601546117492E-5 0.0 0.0 0.0 0.0 7 1.7605082783011487E-4 0.0 0.0 0.0 0.0 8 1.9561203092234984E-4 0.0 0.0 0.0 0.0 9 1.9561203092234984E-4 0.0 0.0 0.0 0.0 10-11 1.9561203092234984E-4 0.0 0.0 2.9341804638352478E-5 0.0 12-13 2.2495383556070235E-4 0.0 0.0 5.8683609276704955E-5 0.0 14-15 3.129792494757598E-4 0.0 0.0 6.846421082282246E-5 0.0 16-17 3.3254045256799473E-4 0.0 0.0 1.6627022628399736E-4 0.0 18-19 4.0100466339081716E-4 0.0 0.0 2.3473443710681982E-4 0.0 20-21 4.4990767112140464E-4 0.0 0.0 3.031986479296423E-4 0.0 22-23 4.6946887421363964E-4 0.0 0.0 5.281524834903446E-4 0.0 24-25 5.477136865825795E-4 0.0 0.0 0.0010954273731651593 0.0 26-27 5.477136865825795E-4 0.0 0.0 0.0017409470752089138 0.0 28-29 6.455197020437545E-4 0.0 0.0 0.005203280022534506 0.0 30-31 7.042033113204595E-4 0.0 0.0 0.017223639322712904 0.0 32-33 7.139839128665769E-4 0.0 0.0 0.04184141341429064 0.0 34-35 7.72667522143282E-4 0.0 0.0 0.07776556289318018 0.0 36-37 9.389377484272793E-4 0.0 0.0 0.1346397608838534 0.0 38-39 9.976213577039842E-4 0.0 0.0 0.2241029232261901 0.0 40-41 0.0010269631623423367 0.0 0.0 0.32751322337329036 0.0 42-43 0.0011247691778035118 0.0 0.0 0.43666473662796157 0.0 44-45 0.0012519169979030391 0.0 0.0 0.5686832962974555 0.0 46-47 0.0014377484272792714 0.0 0.0 0.7293492378955275 0.0 48-49 0.0014670902319176239 0.0 0.0 0.899042674720666 0.0 50-51 0.0014670902319176239 0.0 0.0 1.0870747394447748 0.0 52-53 0.0014670902319176239 0.0 0.0 1.276730384025539 0.0 54-55 0.0014964320365559764 0.0 0.0 1.4865634095959437 0.0 56-57 0.0015648962473787987 0.0 0.0 1.7373771556445807 0.0 58-59 0.0015648962473787987 0.0 0.0 2.0079672780194673 0.0 60-61 0.0015648962473787987 0.0 0.0 2.302118869518951 0.0 62-63 0.0015648962473787987 0.0 0.0 2.6047404619573724 0.0 64-65 0.0015844574504710338 0.0 0.0 2.9195780257268944 0.0 66-67 0.0016235798566555037 0.0 0.0 3.27947482081938 0.0 68-69 0.0016431410597477387 0.0 0.0 3.6854382491940783 0.0 70-71 0.0016822634659322089 0.0 0.0 4.120606553785484 0.0 72-73 0.0017311664736627962 0.0 0.0 4.56028371568965 0.0 74-75 0.001750727676755031 0.0 0.0 5.023062658445745 0.0 76-77 0.0017800694813933837 0.0 0.0 5.511095114393916 0.0 78-79 0.0017800694813933837 0.0 0.0 6.066066007323714 0.0 80-81 0.0019267785045851459 0.0 0.0 6.665401708866702 0.0 82-83 0.0019756815123157336 0.0 0.0 7.267329269819411 0.0 84-85 0.002024584520046321 0.0 0.0 7.880631670370255 0.0 86-87 0.002083268129323026 0.0 0.0 8.514512456574128 0.0 88-89 0.0020930487308691435 0.0 0.0 9.189911896341272 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18600 0.0 24.371567 1 TACATGG 19070 0.0 23.245472 2 ACATGGG 19215 0.0 22.205547 3 AGTACTT 15885 0.0 20.297894 12-13 GAGTACT 14805 0.0 20.16002 12-13 AGAGTAC 25675 0.0 19.904625 10-11 CATGGGG 9230 0.0 19.437136 4 GTACTTT 16220 0.0 18.503693 14-15 CATGGGA 10720 0.0 18.196558 4 CGTATAG 345 1.0641088E-9 17.923704 1 GTATCAA 42240 0.0 17.150606 1 TACTTTT 19590 0.0 16.168344 14-15 GAGTACA 16065 0.0 15.54469 1 AGTACAT 14725 0.0 15.503578 2 CAACGCA 46415 0.0 15.409804 5 ACTTTTT 19630 0.0 15.385663 16-17 TCAACGC 46595 0.0 15.350274 4 ATCAACG 46905 0.0 15.248823 3 AACGCAG 47065 0.0 15.2032 6 TATCAAC 47560 0.0 15.118649 2 >>END_MODULE