##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938771_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2692940 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.946554323527447 31.0 31.0 34.0 30.0 34.0 2 32.160657125669346 33.0 31.0 34.0 30.0 34.0 3 32.24684137039815 34.0 31.0 34.0 30.0 34.0 4 35.8810237138592 37.0 35.0 37.0 35.0 37.0 5 35.800578549837724 37.0 35.0 37.0 35.0 37.0 6 35.846500479030354 37.0 35.0 37.0 35.0 37.0 7 35.78912155488054 37.0 35.0 37.0 33.0 37.0 8 35.76993620355448 37.0 35.0 37.0 33.0 37.0 9 37.38602902404064 39.0 37.0 39.0 34.0 39.0 10-11 37.3062671281202 39.0 37.0 39.0 34.0 39.0 12-13 37.27473411958677 39.0 37.0 39.0 33.0 39.0 14-15 38.39806698255438 40.0 38.0 41.0 33.0 41.0 16-17 38.3687237740165 40.0 38.0 41.0 33.0 41.0 18-19 38.47237220287121 40.0 38.0 41.0 33.5 41.0 20-21 38.55640693814196 40.0 38.0 41.0 34.0 41.0 22-23 38.50671255208063 40.0 38.0 41.0 34.0 41.0 24-25 38.42319249593381 40.0 38.0 41.0 34.0 41.0 26-27 38.23901720795858 40.0 38.0 41.0 33.5 41.0 28-29 38.10845117232468 40.0 37.0 41.0 33.0 41.0 30-31 37.91649609720231 40.0 37.0 41.0 33.0 41.0 32-33 37.683863918245486 40.0 36.5 41.0 33.0 41.0 34-35 37.58730866636464 40.0 36.5 41.0 33.0 41.0 36-37 37.47436816267722 40.0 36.5 41.0 32.5 41.0 38-39 37.356590195102754 40.0 36.0 41.0 32.0 41.0 40-41 37.2686864542099 40.0 36.0 41.0 32.0 41.0 42-43 37.0806445743314 40.0 36.0 41.0 31.0 41.0 44-45 36.794432850342005 40.0 36.0 41.0 30.5 41.0 46-47 36.45281291079638 40.0 36.0 41.0 30.0 41.0 48-49 36.1413421762089 39.5 36.0 41.0 29.5 41.0 50-51 35.92780288457968 39.0 35.0 41.0 28.0 41.0 52-53 35.807145164764165 39.0 35.0 41.0 28.0 41.0 54-55 35.63197471908026 39.0 35.0 41.0 27.5 41.0 56-57 35.39564565122134 39.0 35.0 40.0 27.0 41.0 58-59 35.15040606177635 39.0 35.0 40.0 26.0 41.0 60-61 34.78405720142298 38.0 34.0 40.0 26.0 41.0 62-63 34.49719934346848 38.0 34.0 40.0 25.5 41.0 64-65 34.17320660690547 37.5 34.0 40.0 24.5 41.0 66-67 33.746842113080874 37.0 33.0 39.5 23.0 41.0 68-69 33.28983880071594 36.5 33.0 39.0 22.0 40.5 70-71 32.77393926340728 36.0 32.0 39.0 21.5 40.0 72-73 32.41125387123367 35.5 32.0 38.0 20.5 40.0 74-75 31.980384078367884 35.0 31.5 37.5 19.5 39.5 76-77 29.87028322205471 33.0 29.0 35.5 13.5 38.0 78-79 30.915733919062436 34.5 31.0 36.0 16.5 39.0 80-81 30.901668436727146 35.0 31.0 36.0 15.0 38.0 82-83 30.566499439274548 34.0 31.0 36.0 10.5 37.0 84-85 30.15031935356896 34.0 30.5 35.0 7.0 37.0 86-87 29.680700646876645 34.0 30.0 35.0 2.0 36.0 88-89 29.147198415115078 34.0 29.0 35.0 2.0 36.0 90-91 28.706947796831717 34.0 29.0 35.0 2.0 35.5 92-93 28.456813928271703 34.0 29.0 35.0 2.0 35.0 94-95 28.087696718085066 34.0 29.0 35.0 2.0 35.0 96-97 27.5885103270032 33.5 27.5 35.0 2.0 35.0 98-99 26.94504129315915 33.0 26.5 35.0 2.0 35.0 100-101 23.913263570669976 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12420665987753665 1101 2 0.17278742948808912 1101 3 0.21685682630246106 1101 4 0.18293539394558422 1101 5 0.14635292731735916 1101 6 0.19845950585234107 1101 7 0.23669602252700628 1101 8 0.29585492684675074 1101 9 0.3110515542029759 1101 10-11 0.23900006846316302 1101 12-13 0.2721781563537391 1101 14-15 0.31644876659942867 1101 16-17 0.3258364962620206 1101 18-19 0.3086292380198472 1101 20-21 0.36359917029486155 1101 22-23 0.2971639849826886 1101 24-25 0.28266436063227474 1101 26-27 0.3310379335553719 1101 28-29 0.3722185742538997 1101 30-31 0.25120422151453425 1101 32-33 0.3544081088217439 1101 34-35 0.3642644321909714 1101 36-37 0.3202742317864775 1101 38-39 0.3611767976005993 1101 40-41 0.41738534444396436 1101 42-43 0.39200044190143757 1101 44-45 0.4208438567929562 1101 46-47 0.3678622311124329 1101 48-49 0.28063467537550224 1101 50-51 0.3739180969672802 1101 52-53 0.3492440829392862 1101 54-55 0.3649772011500687 1101 56-57 0.2423708247633627 1101 58-59 0.24397954329788973 1101 60-61 0.2858688973042902 1101 62-63 0.23582910502754117 1101 64-65 0.2572437112783206 1101 66-67 0.28627055769691623 1101 68-69 0.2755691643539464 1101 70-71 0.26537887614702527 1101 72-73 0.35120936969969563 1101 74-75 0.3363679912471369 1101 76-77 0.3417453940112658 1101 78-79 0.33888796441029356 1101 80-81 0.4230050040937421 1101 82-83 0.4122283884154818 1101 84-85 0.426864266854345 1101 86-87 0.41338153707972936 1101 88-89 0.5956379619545267 1101 90-91 0.7290342949847748 1101 92-93 0.6923856130507922 1101 94-95 0.70868023586101 1101 96-97 0.7546362672074949 1101 98-99 0.9676226358910078 1101 100-101 1.5574164724392539 1102 1 0.10271463045069851 1102 2 0.14169929399317738 1102 3 0.16056882228092206 1102 4 0.11080146511521605 1102 5 0.06748727183559566 1102 6 0.0658860112707842 1102 7 0.058276989823006886 1102 8 0.010980648258538395 1102 9 0.15577376836922951 1102 10-11 0.22981481668199422 1102 12-13 0.2057756448256569 1102 14-15 0.2863889752167381 1102 16-17 0.23406687434463436 1102 18-19 0.22056673016528094 1102 20-21 0.25121070514980204 1102 22-23 0.20501661880034305 1102 24-25 0.14376467674839688 1102 26-27 0.11407786859205515 1102 28-29 0.20878363907220177 1102 30-31 0.15394582800313827 1102 32-33 0.1390730934026223 1102 34-35 0.1646334760122059 1102 36-37 0.2349866821214448 1102 38-39 0.27615122796321145 1102 40-41 0.24428687475535327 1102 42-43 0.20756891922710707 1102 44-45 0.06820327212313515 1102 46-47 0.07944421346019936 1102 48-49 0.15043591857224214 1102 50-51 0.10697881639730156 1102 52-53 0.10910990276276067 1102 54-55 0.17232955945367223 1102 56-57 -0.006630799319459868 1102 58-59 0.029021305427754385 1102 60-61 -0.0422886387300494 1102 62-63 -0.0019349292715418187 1102 64-65 0.061387539280211456 1102 66-67 0.10631275484897884 1102 68-69 0.0510527640175269 1102 70-71 -0.03891189268217943 1102 72-73 0.022335980490176155 1102 74-75 0.0822700822537783 1102 76-77 -0.057456581956177644 1102 78-79 -0.004396259751807463 1102 80-81 -0.09027718996482292 1102 82-83 -0.018368655367087428 1102 84-85 0.10287931862198718 1102 86-87 0.016782927217295907 1102 88-89 0.2372720019572334 1102 90-91 0.10750577203010536 1102 92-93 0.22210270362241147 1102 94-95 0.31753003720087847 1102 96-97 0.24354440985609926 1102 98-99 0.24172939033548246 1102 100-101 0.6651071813320719 1103 1 0.004213892255748419 1103 2 0.049476808360026325 1103 3 -0.009858563754285399 1103 4 0.08923089369340431 1103 5 0.05268950570459907 1103 6 0.017931098149226443 1103 7 0.08315834278813838 1103 8 0.06302389486884863 1103 9 0.05736890969022568 1103 10-11 0.027597494624707508 1103 12-13 0.11080690696039852 1103 14-15 0.101753636717973 1103 16-17 0.07607908228132487 1103 18-19 0.1549575372372871 1103 20-21 0.11192065585072442 1103 22-23 0.07906178640315176 1103 24-25 0.18234651664228352 1103 26-27 0.14546240437050528 1103 28-29 0.11617144960005277 1103 30-31 0.0949702604234588 1103 32-33 0.1112568220611152 1103 34-35 0.04686049117926672 1103 36-37 0.04950033559647693 1103 38-39 0.1786277649311856 1103 40-41 0.12529567881458092 1103 42-43 0.21888030512067047 1103 44-45 0.09666487446470029 1103 46-47 0.17904331336804802 1103 48-49 0.12661055475973626 1103 50-51 0.10606950743963495 1103 52-53 0.12596946509751916 1103 54-55 0.01978927033982103 1103 56-57 0.012083258426919485 1103 58-59 0.030338025005995917 1103 60-61 -0.015619132792558332 1103 62-63 0.11189508779037283 1103 64-65 0.1645729970175367 1103 66-67 0.048426424963743386 1103 68-69 -0.0765253704423543 1103 70-71 -0.09958738861510597 1103 72-73 0.014683480640570679 1103 74-75 0.010852446392114246 1103 76-77 -0.08503137609744371 1103 78-79 -2.0763938226409095E-4 1103 80-81 0.09338984274509343 1103 82-83 0.05211701613515274 1103 84-85 -0.05055334588986682 1103 86-87 0.1684448362495914 1103 88-89 0.23641604531085747 1103 90-91 0.332407105049203 1103 92-93 0.3682896603277044 1103 94-95 0.24608796752708884 1103 96-97 0.18730179509179834 1103 98-99 0.295938586139858 1103 100-101 0.7339548595554675 1104 1 0.05976269545329416 1104 2 0.06704487475795418 1104 3 0.09430391190154097 1104 4 0.0759428039965826 1104 5 0.09998078435064883 1104 6 0.09773791026518808 1104 7 0.07702022399774933 1104 8 0.06431744300829934 1104 9 0.11723254477684009 1104 10-11 0.11536601290813309 1104 12-13 0.15347529094619006 1104 14-15 0.1245839350708664 1104 16-17 0.2288273612688556 1104 18-19 0.1329601576863766 1104 20-21 0.18322813554870265 1104 22-23 0.1202087113868231 1104 24-25 0.15177881727549192 1104 26-27 0.2204068426468524 1104 28-29 0.17577427172846427 1104 30-31 0.13054936960598695 1104 32-33 0.03837334701089645 1104 34-35 0.0889397924409181 1104 36-37 0.048851608434333116 1104 38-39 0.2291980604085282 1104 40-41 0.19606673416506482 1104 42-43 0.26296302850072806 1104 44-45 0.16320793542026735 1104 46-47 0.14370263169972475 1104 48-49 0.11125834684132485 1104 50-51 0.06469482183117492 1104 52-53 0.03672463429466433 1104 54-55 -0.02519735924012423 1104 56-57 0.03338022898525139 1104 58-59 0.0253510288456269 1104 60-61 -0.013484327648832561 1104 62-63 -0.029454868663179923 1104 64-65 0.012459548188324732 1104 66-67 -0.14009499542723347 1104 68-69 0.10638849622843338 1104 70-71 -0.07874473740331212 1104 72-73 -0.20523803208660496 1104 74-75 -0.15186749622903406 1104 76-77 -0.13747093126712073 1104 78-79 -0.16106335528003157 1104 80-81 -0.20192854963147866 1104 82-83 -0.13935004323329636 1104 84-85 -0.13171199844238402 1104 86-87 -0.0364274983614159 1104 88-89 0.12279046124232096 1104 90-91 0.0426351195760013 1104 92-93 0.15786618632013472 1104 94-95 0.3434941706387882 1104 96-97 0.1730102666001656 1104 98-99 0.35400725416537426 1104 100-101 1.0039384130148363 1105 1 -0.02759484007197699 1105 2 -0.021050752213263024 1105 3 0.006900001103801401 1105 4 -0.05929864958416431 1105 5 -0.006658140241569299 1105 6 -0.0555454083029332 1105 7 -0.05337805698683695 1105 8 0.002736733150698001 1105 9 0.00808500778929755 1105 10-11 0.05757242373883997 1105 12-13 0.03577890724645982 1105 14-15 0.005794978936229711 1105 16-17 -0.06758577226627693 1105 18-19 -0.10969251369597544 1105 20-21 0.04212100707513855 1105 22-23 0.05231451541159515 1105 24-25 -0.00230585150868734 1105 26-27 -0.08801313877695094 1105 28-29 -0.09673871298551973 1105 30-31 -0.05954388671927546 1105 32-33 -0.07327425193111026 1105 34-35 0.09938893840404006 1105 36-37 -0.029219206316888346 1105 38-39 -0.04587223073688307 1105 40-41 -0.024790790998224566 1105 42-43 -0.17779234353112372 1105 44-45 -0.08181639638089422 1105 46-47 0.009078331549687846 1105 48-49 -0.03646312733003754 1105 50-51 -0.029380751602694488 1105 52-53 -0.14697513856794586 1105 54-55 -0.12158031986360385 1105 56-57 -0.036700488625719174 1105 58-59 0.013131361933126584 1105 60-61 -0.14189883389202862 1105 62-63 -0.23635336435077647 1105 64-65 -0.13963598371358188 1105 66-67 -0.10462624504227591 1105 68-69 -0.07710577620802894 1105 70-71 -0.09036572853273839 1105 72-73 -0.24702034105055048 1105 74-75 -0.1730443296355766 1105 76-77 -0.13190466672720547 1105 78-79 -0.27237190750344453 1105 80-81 -0.009584933209470847 1105 82-83 -0.1097147224345214 1105 84-85 -0.030487459372739778 1105 86-87 -0.08130637122200923 1105 88-89 0.011227859479394198 1105 90-91 0.18792131225163544 1105 92-93 -0.03949175643799663 1105 94-95 -0.021754939209596813 1105 96-97 -3.027495330698571E-4 1105 98-99 0.07330367171905294 1105 100-101 0.3047871239779063 1106 1 0.02059809947174429 1106 2 -0.015024454567175383 1106 3 -0.10292097430971126 1106 4 -0.07442558353243811 1106 5 0.02886655789219361 1106 6 -0.09006901482160856 1106 7 -0.010395550648389928 1106 8 -0.006464372677157826 1106 9 -0.002734709665027424 1106 10-11 -0.05380164909824714 1106 12-13 -0.004599968091618223 1106 14-15 0.10213588457747136 1106 16-17 0.03152763101710576 1106 18-19 -0.10097443997786826 1106 20-21 -0.01730743808182922 1106 22-23 -0.0935314156519027 1106 24-25 -0.10535536828198389 1106 26-27 -0.0753649336138622 1106 28-29 -0.04127499423214687 1106 30-31 0.04879851457734219 1106 32-33 0.0010628185706735849 1106 34-35 -0.10169159188478005 1106 36-37 -0.07147954289412439 1106 38-39 -0.016723455549772837 1106 40-41 -0.1535468125030306 1106 42-43 -0.1279422333966309 1106 44-45 -0.1409948929618352 1106 46-47 -0.153761652207983 1106 48-49 -0.14892895940293727 1106 50-51 -0.1757202374091733 1106 52-53 -0.3229221431274141 1106 54-55 -0.23326740500338872 1106 56-57 -0.2584036503310543 1106 58-59 -0.23481459843518593 1106 60-61 -0.2709065337207974 1106 62-63 -0.24918045427908453 1106 64-65 -0.1941001964541229 1106 66-67 -0.21513414660289953 1106 68-69 -0.2734826894353688 1106 70-71 -0.34075978673327967 1106 72-73 -0.23459599409886778 1106 74-75 0.015707278129092117 1106 76-77 -0.06802082333685178 1106 78-79 -0.16298762136969458 1106 80-81 -0.20164259378017135 1106 82-83 0.010218287311023033 1106 84-85 -0.10349579517216512 1106 86-87 0.056254715907268604 1106 88-89 0.22412516275912608 1106 90-91 0.3478078234898838 1106 92-93 0.17043275880374864 1106 94-95 0.20215920071576576 1106 96-97 0.13319220472014237 1106 98-99 0.2558098083602012 1106 100-101 0.7467634503594311 1107 1 -0.12513696099495775 1107 2 -0.17045497370056495 1107 3 -0.2082166125878473 1107 4 -0.07024594221559965 1107 5 -0.11639923318775658 1107 6 -0.027739589721825553 1107 7 -0.04520144337418941 1107 8 -0.033096451122801795 1107 9 -0.21660900576094377 1107 10-11 -0.18033355740682566 1107 12-13 -0.17677876762329703 1107 14-15 -0.16912033909162716 1107 16-17 -0.16879850331137192 1107 18-19 -0.14632956306741818 1107 20-21 -0.13882918869637706 1107 22-23 -0.15397629853409 1107 24-25 -0.16811817492921222 1107 26-27 -0.09847749165884778 1107 28-29 -0.1274038706212295 1107 30-31 -0.2563791819057073 1107 32-33 -0.1446600400306579 1107 34-35 -0.2153661793325341 1107 36-37 -0.14360419485386444 1107 38-39 -0.17077186221583673 1107 40-41 -0.2307299271885057 1107 42-43 -0.275318947184509 1107 44-45 -0.29813080221899924 1107 46-47 -0.3353139247120893 1107 48-49 -0.4479696200377674 1107 50-51 -0.42917121203675634 1107 52-53 -0.3334209869647893 1107 54-55 -0.526689465516597 1107 56-57 -0.5292854202848432 1107 58-59 -0.5133152848977716 1107 60-61 -0.5061536889635505 1107 62-63 -0.6317773356545615 1107 64-65 -0.558882067295805 1107 66-67 -0.46047497197726983 1107 68-69 -0.271848285457736 1107 70-71 -0.2754574130254568 1107 72-73 -0.18649494887272766 1107 74-75 -0.22914102253865565 1107 76-77 -0.38319457502860743 1107 78-79 -0.24573679638004364 1107 80-81 -0.24184580221042395 1107 82-83 -0.2365569750003722 1107 84-85 -0.2595967709194511 1107 86-87 -0.2156676578350094 1107 88-89 0.010200193034108906 1107 90-91 0.15366588163047723 1107 92-93 0.105355042969979 1107 94-95 0.2966073089098522 1107 96-97 0.17371940856364532 1107 98-99 0.060423491560730014 1107 100-101 0.36531526694443883 1108 1 -0.19891085512599815 1108 2 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-0.7352382634808734 2316 62-63 -0.7204026204023819 2316 64-65 -0.7676392020956868 2316 66-67 -0.9354559331198686 2316 68-69 -1.0883873027091369 2316 70-71 -0.9854944218921275 2316 72-73 -1.1738143063804785 2316 74-75 -1.0497586020366185 2316 76-77 -1.1327615483847033 2316 78-79 -1.0715212307064839 2316 80-81 -1.064643068440457 2316 82-83 -1.3384728375655897 2316 84-85 -1.2750007096248268 2316 86-87 -1.4885547263949341 2316 88-89 -1.8146294354361885 2316 90-91 -2.091919035968008 2316 92-93 -2.1176596431942585 2316 94-95 -2.0236390367270083 2316 96-97 -2.2031355678310334 2316 98-99 -2.5906247907907733 2316 100-101 -3.3647572763510674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 14.0 5 62.0 6 182.0 7 468.0 8 965.0 9 1853.0 10 3109.0 11 5110.0 12 7386.0 13 10438.0 14 12485.0 15 16752.0 16 21949.0 17 30637.0 18 27227.0 19 16554.0 20 14608.0 21 14692.0 22 15883.0 23 17564.0 24 20403.0 25 23880.0 26 28638.0 27 35137.0 28 43336.0 29 52959.0 30 64338.0 31 78638.0 32 96987.0 33 122165.0 34 159303.0 35 218732.0 36 320453.0 37 548884.0 38 589629.0 39 71504.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.45071621818605 23.2892911559366 15.985852719608493 20.27413990626886 2 13.951629074543066 24.52676257176172 39.8252096221973 21.69639873149792 3 19.341871708987206 28.602159721345444 32.489175399377636 19.56679317028972 4 10.961105706031326 19.47284380639747 42.416912370866044 27.149138116705164 5 10.250989624722422 41.05490653337988 36.78849138859388 11.905612453303824 6 25.026699443730642 42.004946266905314 18.608138317229496 14.360215972134544 7 23.66669884958447 35.82103574531924 24.328020676286883 16.184244728809404 8 23.01354653278573 34.4121294941588 22.05370338737588 20.52062058567959 9 23.85311221193194 19.540799275141666 24.868508024686772 31.73758048823962 10-11 22.23811150638336 28.000549585211704 29.849161139869434 19.912177768535503 12-13 25.394568761279494 26.818161563198583 28.810816431112464 18.976453244409456 14-15 21.618914837666807 28.559691503707757 29.6946991078688 20.12669455075664 16-17 18.246178029544154 31.435399475915137 30.370147698499512 19.948274796041197 18-19 17.83635036257824 31.748062337909253 32.12092675058004 18.294660548932466 20-21 19.60537553751662 29.092813059332922 31.289167229867726 20.01264417328273 22-23 19.011507868723402 29.21706387814062 31.42702770949223 20.34440054364375 24-25 20.336936582322664 29.75205537442349 31.237959256426063 18.673048786827778 26-27 19.728990909734005 28.239703476002305 33.76727738187565 18.26402823238804 28-29 18.04990085185708 30.582170415976588 32.76575044375292 18.602178288413405 30-31 19.058519684805457 29.230116526918536 31.946032960259046 19.765330828016964 32-33 19.029536491715373 29.757346988792914 32.26748460790066 18.94563191159105 34-35 20.327250310093063 28.817208493950403 31.99917184726338 18.856369348693153 36-37 19.950525359328104 28.10739722201434 32.947024325619715 18.99505309303784 38-39 18.98136955064463 29.848846947321118 32.54715176769224 18.62263173434201 40-41 18.740924637821095 29.78614640160729 32.70162623062839 18.77130272994322 42-43 18.290927145481024 30.096856634207825 31.076619309087857 20.535596911223294 44-45 19.165999631993675 28.83788170212023 31.237739698468765 20.75837896741733 46-47 20.36774481042742 29.954677113892085 30.672156411303796 19.005421664376694 48-49 19.66152600160531 29.57370561054194 30.925834018922444 19.838934368930307 50-51 18.008205195237302 29.86362799153932 32.23405898709509 19.894107826128288 52-53 19.02545173676354 31.062816104332068 30.199466011125388 19.712266147779005 54-55 18.403937703773572 29.445160308064793 30.89040231122862 21.260499676933016 56-57 19.70413377201126 29.556414179298464 30.074806716822504 20.664645331867774 58-59 20.56628397862621 28.922700952069025 30.16757432300831 20.34344074629645 60-61 19.41896254425254 29.953791871996412 31.3139673986404 19.31327818511065 62-63 18.76967180850669 29.63699525425743 31.45220465364991 20.141128283585967 64-65 18.469442319546665 30.86108491091521 30.38916574450229 20.280307025035835 66-67 19.071210936847248 29.50887792596499 29.335905515027395 22.084005622160372 68-69 18.419402107151928 29.65811511947608 30.285555775060864 21.63692699831113 70-71 20.017936070719365 29.48120008281043 29.711323954959294 20.789539891510913 72-73 19.538311288034638 28.170921000839233 31.114339717929102 21.176427993197024 74-75 18.38111877724717 31.152940652223965 29.38024983846651 21.085690732062357 76-77 18.985747405349766 29.163994978899705 29.875862699595984 21.974394916154537 78-79 18.998041632398948 29.060715339085107 30.309991909475276 21.631251119040666 80-81 19.708003040544178 29.25389323709152 29.556945032917564 21.481158689446733 82-83 19.188674831225352 28.89338418234346 30.54416362785654 21.373777358574646 84-85 18.637808491834203 29.263091639620637 29.983642413124688 22.11545745542047 86-87 18.629600373095194 29.73561638435351 29.841888266319756 21.792894976231544 88-89 18.97471948570563 29.1594229462807 29.14832103088363 22.71753653713004 90-91 19.26500674380899 27.969845380211556 29.821488285285092 22.943659590694363 92-93 19.939619894984663 29.222355492510044 29.63495287678151 21.203071735723782 94-95 19.352532799195824 28.23604095079002 29.84300241427629 22.56842383573786 96-97 18.29313283866942 28.344416946611684 30.485794426499368 22.876655788219526 98-99 19.0647829980395 28.996450529004303 28.700305410097858 23.238461062858335 100-101 18.869037506579737 27.867738734147128 29.805611179654857 23.457612579618278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3882.0 1 2371.5 2 998.5 3 1109.5 4 899.0 5 867.5 6 1517.5 7 2978.0 8 4339.5 9 4561.0 10 3847.0 11 3237.5 12 3240.5 13 3416.5 14 3611.0 15 3801.0 16 4040.5 17 4375.0 18 4801.5 19 5398.0 20 6145.5 21 7094.0 22 8338.0 23 9690.0 24 11046.0 25 13705.5 26 19402.0 27 26016.5 28 29137.5 29 33402.5 30 43196.5 31 52356.5 32 64764.0 33 81883.0 34 98866.0 35 112028.0 36 125159.5 37 136356.5 38 134181.5 39 127308.5 40 119892.5 41 110133.0 42 101413.5 43 98792.0 44 97601.0 45 93807.0 46 91262.0 47 114450.0 48 178391.5 49 168767.0 50 95113.5 51 55877.0 52 34578.5 53 24842.0 54 20294.0 55 17086.0 56 14267.0 57 11978.5 58 9727.5 59 7699.0 60 6303.5 61 5159.5 62 3929.0 63 2752.0 64 1943.5 65 1476.5 66 1022.5 67 789.5 68 556.5 69 360.5 70 265.0 71 202.5 72 153.5 73 116.0 74 127.5 75 105.0 76 64.5 77 60.5 78 56.0 79 38.0 80 24.0 81 17.0 82 7.0 83 2.5 84 3.0 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3931390970463508 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.4137188351764245E-4 16-17 0.0020609445438814085 18-19 1.4853654370316458E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.6710361166606013E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007426827185158229 36-37 0.016691794098643117 38-39 0.011325911457366299 40-41 0.00779816854441614 42-43 0.0034906087770243677 44-45 0.001912408000178244 46-47 0.0014853654370316457 48-49 1.8567067962895572E-5 50-51 4.4560963110949373E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.755791068497627E-4 60-61 5.755791068497627E-4 62-63 0.0 64-65 0.0 66-67 9.283533981447786E-4 68-69 5.941461748126583E-4 70-71 0.0015782007768461233 72-73 0.0 74-75 0.0 76-77 0.10000222804815555 78-79 0.03349499060506361 80-81 3.7134135925791145E-5 82-83 0.0 84-85 0.0 86-87 0.15158154284907946 88-89 0.3237539640690101 90-91 0.27902589734639466 92-93 0.0 94-95 1.8567067962895572E-5 96-97 1.8567067962895572E-5 98-99 1.29969475740269E-4 100-101 4.641766990723893E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2692940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.30068746448529 #Duplication Level Percentage of deduplicated Percentage of total 1 69.05926888571777 15.400691719462456 2 12.61578019040234 5.626811422936147 3 5.37315260940898 3.5947499112423973 4 2.992701240835911 2.669571801858359 5 1.9842391941559618 2.212494906182713 6 1.3023635179769402 1.7426161067730785 7 0.9038099409876538 1.4108908114882392 8 0.7254003460260336 1.2941541122684848 9 0.5604311926583163 1.124820078553967 >10 3.5621772997991004 14.943550133945882 >50 0.42071230134794974 6.636635753009395 >100 0.4327796587270115 20.006590562591757 >500 0.04484465088376083 6.793983666007304 >1k 0.018838087545966446 7.215164107451695 >5k 0.001667087393448358 2.7093624354438086 >10k+ 0.001833796132793194 6.6179124707843675 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 43484 1.614740766597102 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 22294 0.8278684263295878 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 17372 0.6450942093028438 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14157 0.5257079623014251 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14149 0.5254108892140188 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11671 0.4333925003899084 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11372 0.4222893937480969 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11334 0.42087829658291676 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11314 0.420135613864401 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10828 0.4020884238044665 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10035 0.3726410540153141 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9188 0.341188440886169 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8987 0.333724479565085 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8493 0.31538021641774416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8047 0.2988183917948413 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7361 0.27334437454974864 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7054 0.2619441948205307 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6181 0.22952609415731506 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5968 0.22161652320512154 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5862 0.2176803047969877 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5736 0.21300140367033799 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4972 0.18463092382303356 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4612 0.17126263488974877 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4546 0.16881178191864654 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4408 0.16368727116088735 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4109 0.1525841645190758 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4096 0.15210142075204053 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3743 0.13899307077023623 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3458 0.12840984203138578 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3196 0.1186806984188285 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3182 0.11816082051586742 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3022 0.11221935876774083 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2788 0.10352997096110571 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2779 0.10319576373777359 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2736 0.10159899589296456 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.426827185158229E-5 0.0 14-15 0.0 0.0 0.0 1.1140240777737343E-4 0.0 16-17 0.0 0.0 0.0 2.9707308740632916E-4 0.0 18-19 0.0 0.0 0.0 5.19877902961076E-4 0.0 20-21 0.0 0.0 0.0 0.0010026216699963609 0.0 22-23 0.0 0.0 0.0 0.002283749359436155 0.0 24-25 0.0 0.0 0.0 0.004790303534427057 0.0 26-27 0.0 0.0 0.0 0.009005027962004352 1.8567067962895572E-5 28-29 0.0 3.7134135925791145E-5 0.0 0.02246615223510364 3.7134135925791145E-5 30-31 0.0 3.7134135925791145E-5 0.0 0.059748824704597955 3.7134135925791145E-5 32-33 0.0 3.7134135925791145E-5 0.0 0.13331154797359018 3.7134135925791145E-5 34-35 0.0 5.570120388868672E-5 0.0 0.24395270596448493 3.7134135925791145E-5 36-37 0.0 7.426827185158229E-5 0.0 0.4022555274161326 3.7134135925791145E-5 38-39 0.0 7.426827185158229E-5 0.0 0.6261557999806903 3.7134135925791145E-5 40-41 0.0 7.426827185158229E-5 0.0 0.8665064947603734 3.7134135925791145E-5 42-43 0.0 7.426827185158229E-5 0.0 1.1104034995209697 3.7134135925791145E-5 44-45 0.0 7.426827185158229E-5 0.0 1.4035589355871279 3.7134135925791145E-5 46-47 0.0 7.426827185158229E-5 0.0 1.756964507192882 3.7134135925791145E-5 48-49 0.0 7.426827185158229E-5 0.0 2.1062667567788367 3.7134135925791145E-5 50-51 0.0 7.426827185158229E-5 0.0 2.4734490928130595 3.7134135925791145E-5 52-53 0.0 9.283533981447787E-5 0.0 2.835599753429338 3.7134135925791145E-5 54-55 0.0 1.1140240777737343E-4 0.0 3.225081138086998 3.7134135925791145E-5 56-57 0.0 1.1140240777737343E-4 0.0 3.7108142030643085 3.7134135925791145E-5 58-59 0.0 1.29969475740269E-4 0.0 4.203769857479187 3.7134135925791145E-5 60-61 0.0 1.4853654370316458E-4 0.0 4.745259827549073 3.7134135925791145E-5 62-63 0.0 1.4853654370316458E-4 0.0 5.263949438160523 3.7134135925791145E-5 64-65 0.0 1.4853654370316458E-4 0.0 5.7948376124235965 3.7134135925791145E-5 66-67 0.0 1.4853654370316458E-4 0.0 6.405489910655269 3.7134135925791145E-5 68-69 0.0 1.4853654370316458E-4 0.0 7.081999598951332 3.7134135925791145E-5 70-71 0.0 1.4853654370316458E-4 0.0 7.816995551330516 3.7134135925791145E-5 72-73 0.0 1.4853654370316458E-4 0.0 8.515971391861683 3.7134135925791145E-5 74-75 0.0 1.4853654370316458E-4 0.0 9.25926682362028 3.7134135925791145E-5 76-77 0.0 1.4853654370316458E-4 0.0 10.003100700349805 3.7134135925791145E-5 78-79 0.0 1.4853654370316458E-4 0.0 10.90291651503561 3.7134135925791145E-5 80-81 0.0 1.4853654370316458E-4 0.0 11.856818198697335 3.7134135925791145E-5 82-83 0.0 1.4853654370316458E-4 0.0 12.73827118316784 3.7134135925791145E-5 84-85 0.0 1.4853654370316458E-4 0.0 13.663059704263741 3.7134135925791145E-5 86-87 0.0 1.4853654370316458E-4 0.0 14.606378159186614 3.7134135925791145E-5 88-89 0.0 1.4853654370316458E-4 0.0 15.595817210929319 3.7134135925791145E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 690 0.0 44.69755 9 TCGCAAC 30 0.003971968 31.634462 12-13 TAGGCAT 965 0.0 30.484833 5 GATCGTG 80 4.5240592E-4 29.655106 7 GGCATTC 1080 0.0 28.996103 7 GTACACG 105 6.7746354E-5 27.221401 1 GGTTCGC 35 0.008436414 27.115002 10-11 CCTTTAG 1235 0.0 27.000984 1 CTACGGG 80 6.3077096E-8 26.693806 42-43 ATTGCCG 215 5.820766E-11 26.4827 8 CTTTGCG 490 0.0 26.14491 2 ATGGGCG 185 9.084033E-9 25.64766 5 TTAGGCA 1290 0.0 24.643621 4 CATGGGG 3670 0.0 21.978716 4 CGATAGC 65 2.9634062E-4 21.901186 54-55 GTGTACG 195 4.2704596E-7 21.899155 9 GAGTACT 12225 0.0 21.581303 12-13 TCGTGCA 110 0.0028966507 21.567348 9 TGGGCGA 135 3.8258728E-4 21.088076 6 CTTTAGG 1670 0.0 20.740816 2 >>END_MODULE