##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938770_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2682336 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.439496394187753 33.0 31.0 34.0 30.0 34.0 2 31.50840237762905 33.0 31.0 34.0 28.0 34.0 3 31.5111197851425 33.0 31.0 34.0 28.0 34.0 4 35.06370380146261 37.0 35.0 37.0 32.0 37.0 5 35.11931950359686 37.0 35.0 37.0 32.0 37.0 6 35.20031122126385 37.0 35.0 37.0 32.0 37.0 7 35.13010376030445 37.0 35.0 37.0 32.0 37.0 8 35.1038024319101 37.0 35.0 37.0 32.0 37.0 9 36.61734957887453 39.0 37.0 39.0 32.0 39.0 10-11 36.502986948689504 39.0 37.0 39.0 32.0 39.0 12-13 36.43755871747611 39.0 37.0 39.0 32.0 39.0 14-15 37.4922282294239 40.0 37.0 41.0 32.0 41.0 16-17 37.41631697147561 40.0 37.0 41.0 32.0 41.0 18-19 37.45535551847345 40.0 37.0 41.0 32.0 41.0 20-21 37.35653176932345 40.0 37.0 41.0 31.5 41.0 22-23 37.38033751178078 40.0 37.0 41.0 32.0 41.0 24-25 37.302451855397685 40.0 37.0 41.0 31.0 41.0 26-27 37.15534500524916 40.0 37.0 41.0 31.0 41.0 28-29 37.01443145079513 40.0 36.0 41.0 30.5 41.0 30-31 36.82668744706107 39.0 36.0 41.0 30.0 41.0 32-33 36.62415465474869 39.0 35.5 41.0 30.0 41.0 34-35 36.31156834937905 39.0 35.0 41.0 30.0 41.0 36-37 36.07252260716032 39.0 35.0 41.0 29.5 41.0 38-39 35.899317982534626 39.0 35.0 40.5 29.0 41.0 40-41 35.85324489549407 39.0 35.0 40.5 29.0 41.0 42-43 35.801289808584755 39.0 35.0 41.0 27.5 41.0 44-45 35.580127359137705 39.0 35.0 41.0 26.5 41.0 46-47 35.218671710031856 39.0 35.0 41.0 24.5 41.0 48-49 34.890889135440155 39.0 35.0 41.0 24.0 41.0 50-51 33.76685881261706 38.0 33.0 40.0 22.0 40.5 52-53 33.90946212555026 38.0 33.5 39.5 22.5 40.5 54-55 34.47955904853083 39.0 34.0 40.0 22.5 41.0 56-57 34.3870040889732 39.0 34.0 40.0 21.5 41.0 58-59 34.23502126504658 38.5 34.0 40.0 20.5 41.0 60-61 33.89868923952853 38.0 34.0 40.0 19.0 41.0 62-63 33.563382812593204 38.0 33.0 40.0 16.5 41.0 64-65 33.19424822244491 37.5 33.0 40.0 13.0 41.0 66-67 32.75537665676485 37.0 32.0 39.5 8.5 41.0 68-69 32.29653313380576 36.5 32.0 39.0 7.5 41.0 70-71 31.798692632093818 36.0 31.0 39.0 4.0 40.0 72-73 31.325746103396444 35.0 31.0 38.5 2.0 40.0 74-75 30.82427406559059 35.0 30.5 37.5 2.0 39.5 76-77 30.326020677499017 35.0 30.0 37.0 2.0 39.0 78-79 29.814880760650418 34.5 29.5 36.5 2.0 39.0 80-81 29.244952906720115 34.0 29.0 36.0 2.0 38.0 82-83 28.768024214714337 34.0 29.0 35.5 2.0 37.0 84-85 28.332527319470792 34.0 28.0 35.0 2.0 37.0 86-87 27.916961745284706 34.0 27.0 35.0 2.0 36.0 88-89 27.365261473581235 34.0 26.0 35.0 2.0 36.0 90-91 26.817134952518998 34.0 25.0 35.0 2.0 36.0 92-93 26.29547957452012 33.0 24.5 35.0 2.0 35.0 94-95 25.719267645813204 33.0 21.5 35.0 2.0 35.0 96-97 25.04738612164919 32.5 16.5 35.0 2.0 35.0 98-99 24.27486582590697 32.0 2.0 35.0 2.0 35.0 100-101 21.84584462945731 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34120113546951814 1101 2 0.4190683776780695 1101 3 0.5131089879375459 1101 4 0.2716683501470243 1101 5 0.29192081192458375 1101 6 0.31783030026931414 1101 7 0.4211918465915687 1101 8 0.49109179637387257 1101 9 0.5495143157726332 1101 10-11 0.48616005195363243 1101 12-13 0.4495120006772453 1101 14-15 0.5257057380195462 1101 16-17 0.6390889889847742 1101 18-19 0.6031268828478247 1101 20-21 0.7522397289404168 1101 22-23 0.58074187441931 1101 24-25 0.619593049671451 1101 26-27 0.6398328674999192 1101 28-29 0.6317757537659929 1101 30-31 0.6739511758965335 1101 32-33 0.4881930774406129 1101 34-35 0.6707007277464214 1101 36-37 0.6326841247510302 1101 38-39 0.6155091242029371 1101 40-41 0.7204529426278157 1101 42-43 0.9206522459989799 1101 44-45 0.9448887241776305 1101 46-47 0.8545171187960037 1101 48-49 0.8113928776069201 1101 50-51 0.9271348938511181 1101 52-53 0.9422014321481953 1101 54-55 0.9749304032686794 1101 56-57 0.8575098909384096 1101 58-59 0.7465190046653731 1101 60-61 0.7506250436294337 1101 62-63 0.7023630237139855 1101 64-65 0.6800404116946339 1101 66-67 0.6202336210469426 1101 68-69 0.6790412573431084 1101 70-71 0.5343385157227409 1101 72-73 0.4482026188509636 1101 74-75 0.47984849069405655 1101 76-77 0.375272362052808 1101 78-79 0.18585594636738634 1101 80-81 0.41861127366606965 1101 82-83 0.5052829317158576 1101 84-85 0.4821891382223562 1101 86-87 0.23610445818995984 1101 88-89 0.30845616940808185 1101 90-91 0.3132537270025928 1101 92-93 0.390300000776751 1101 94-95 0.351186886018958 1101 96-97 0.38971389214501073 1101 98-99 0.45448403419689143 1101 100-101 0.8523419013581908 1102 1 0.3752463157396555 1102 2 0.3439610868070737 1102 3 0.4142476976248588 1102 4 0.5164119410704799 1102 5 0.5651816874917088 1102 6 0.5050371484791967 1102 7 0.5097432434435518 1102 8 0.5304186937887678 1102 9 0.6600808177067847 1102 10-11 0.6833467150618304 1102 12-13 0.6597531022070768 1102 14-15 0.7900999702341096 1102 16-17 0.8675440004424289 1102 18-19 0.9671351286766026 1102 20-21 0.9676996609000597 1102 22-23 0.8709366851916585 1102 24-25 0.9743482823062877 1102 26-27 0.8613395104843633 1102 28-29 0.966045178858721 1102 30-31 1.0688272123769664 1102 32-33 1.001807207625319 1102 34-35 1.0439810828569591 1102 36-37 0.973464472192461 1102 38-39 1.002064174619676 1102 40-41 1.1072630676956834 1102 42-43 1.2585460932241403 1102 44-45 1.2712539027298888 1102 46-47 1.2024335745621144 1102 48-49 1.1485196602919103 1102 50-51 1.1409515652726299 1102 52-53 1.1172159267690844 1102 54-55 1.1621167749506398 1102 56-57 1.2005782411284471 1102 58-59 1.1394910308139714 1102 60-61 1.1021779620331529 1102 62-63 0.9325011750899748 1102 64-65 0.9383937614091877 1102 66-67 0.9456153894213699 1102 68-69 1.1651163747572113 1102 70-71 1.1731598653023205 1102 72-73 1.1564713015736778 1102 74-75 0.9482301240921167 1102 76-77 1.0130672010474768 1102 78-79 1.0271475167867052 1102 80-81 1.0919034392084477 1102 82-83 0.9926389441459484 1102 84-85 0.9547177670158824 1102 86-87 0.8985383887937068 1102 88-89 0.9700079221068414 1102 90-91 0.7466164330332745 1102 92-93 0.7359711707457386 1102 94-95 0.85313622080022 1102 96-97 0.8236312283251195 1102 98-99 0.7551211072957962 1102 100-101 1.0810186268316038 1103 1 0.31612901339027744 1103 2 0.4418288576638325 1103 3 0.4561004286510517 1103 4 0.4379510844902583 1103 5 0.4364858335589261 1103 6 0.32694931301303853 1103 7 0.3438633717104622 1103 8 0.44888591612006223 1103 9 0.6358511033326764 1103 10-11 0.5921171732174244 1103 12-13 0.5476877729430711 1103 14-15 0.6890720799154124 1103 16-17 0.6605467311711593 1103 18-19 0.7004071842192658 1103 20-21 0.7476932059094921 1103 22-23 0.6740063752926133 1103 24-25 0.7857966691535694 1103 26-27 0.8429778882292212 1103 28-29 0.8264085621066783 1103 30-31 0.9406190523169968 1103 32-33 0.8110100311631356 1103 34-35 0.9146986279377813 1103 36-37 0.9084689639140109 1103 38-39 0.8757731765056036 1103 40-41 0.95357346052797 1103 42-43 1.145340729335203 1103 44-45 1.2178455487227353 1103 46-47 1.0307492682207524 1103 48-49 0.8580427049057064 1103 50-51 0.8687508596856475 1103 52-53 0.8758230766378929 1103 54-55 0.8676476904884254 1103 56-57 0.8827722834993565 1103 58-59 0.8839236758132927 1103 60-61 0.8465069404788395 1103 62-63 0.7225288095887734 1103 64-65 0.6324765875646889 1103 66-67 0.5908552057069798 1103 68-69 0.5561994150875123 1103 70-71 0.48922178561739926 1103 72-73 0.47464611580678806 1103 74-75 0.4601120661816296 1103 76-77 0.4881457318083342 1103 78-79 0.4679266966161961 1103 80-81 0.5524345927182353 1103 82-83 0.4844558306392557 1103 84-85 0.5341322963965034 1103 86-87 0.4882966687320689 1103 88-89 0.5369888605494353 1103 90-91 0.4769904357818504 1103 92-93 0.061195161893181194 1103 94-95 0.11196617195646752 1103 96-97 0.07543936934732898 1103 98-99 0.23522114477638567 1103 100-101 0.7781292005226099 1104 1 0.10392436291102314 1104 2 0.14604574550974192 1104 3 0.0761536376356986 1104 4 0.3374841354108611 1104 5 0.2522019037919847 1104 6 0.29485713387067847 1104 7 0.3423549868049278 1104 8 0.34996700791897695 1104 9 0.43656817662857605 1104 10-11 0.37901982596873296 1104 12-13 0.3639383240444545 1104 14-15 0.49201586524129226 1104 16-17 0.48366490946907703 1104 18-19 0.4556662660202093 1104 20-21 0.5637583070205565 1104 22-23 0.37249414142501536 1104 24-25 0.3946632489658768 1104 26-27 0.42965228586665916 1104 28-29 0.4507389301840874 1104 30-31 0.471668804233893 1104 32-33 0.4769459296158516 1104 34-35 0.5506983491796262 1104 36-37 0.5778502665893939 1104 38-39 0.6330563166521443 1104 40-41 0.7142044686994353 1104 42-43 0.6513932221796352 1104 44-45 0.7262826485181577 1104 46-47 0.7613930712751937 1104 48-49 0.9747104310089085 1104 50-51 1.0308793473701314 1104 52-53 0.9422009059166001 1104 54-55 0.9167623417374742 1104 56-57 0.9454852885589915 1104 58-59 0.9109511165800939 1104 60-61 0.9370725804445996 1104 62-63 1.0514692719774175 1104 64-65 1.0406200873675715 1104 66-67 0.986265056017352 1104 68-69 0.8920634895754276 1104 70-71 1.0448599901815676 1104 72-73 0.8786700598498776 1104 74-75 0.8583474045520489 1104 76-77 0.8018625286787788 1104 78-79 0.5888272604308185 1104 80-81 0.6265874711586825 1104 82-83 0.5945954838127818 1104 84-85 0.695564471853185 1104 86-87 0.6336524294619252 1104 88-89 0.8917802059832809 1104 90-91 0.7774710311812925 1104 92-93 0.7848189830027508 1104 94-95 0.8164803338041544 1104 96-97 0.8842026686776094 1104 98-99 0.9904103912429214 1104 100-101 1.3913682887151602 1105 1 0.1048526679904569 1105 2 0.12117735331847257 1105 3 0.22852884523134875 1105 4 0.2730707302154869 1105 5 0.34458196834248156 1105 6 0.3346058256263902 1105 7 0.2360307615675623 1105 8 0.31561580308081716 1105 9 0.21914110928925368 1105 10-11 0.3913560995876395 1105 12-13 0.36776905133427107 1105 14-15 0.45972299086408697 1105 16-17 0.5436776785600443 1105 18-19 0.5180350499013855 1105 20-21 0.4789887773617991 1105 22-23 0.5229383177794773 1105 24-25 0.4731911999576397 1105 26-27 0.43974025941975725 1105 28-29 0.6271071030290045 1105 30-31 0.6619360934263838 1105 32-33 0.6831177874794179 1105 34-35 0.5454712473715801 1105 36-37 0.6448782019135564 1105 38-39 0.6855133874383483 1105 40-41 0.7160336605648183 1105 42-43 0.7127945319906814 1105 44-45 0.7639357992717777 1105 46-47 0.7779028008524804 1105 48-49 0.731508209343751 1105 50-51 0.8251833927434973 1105 52-53 0.8447778338143266 1105 54-55 0.7634882058054018 1105 56-57 0.556991571265101 1105 58-59 0.6173266531952706 1105 60-61 0.7662781549753461 1105 62-63 0.7133077077246028 1105 64-65 0.7392754159170707 1105 66-67 0.7795526660822105 1105 68-69 0.7600111128010738 1105 70-71 0.5979418063693238 1105 72-73 0.8542344850057653 1105 74-75 0.7062757566342732 1105 76-77 0.6247618957078878 1105 78-79 0.655817985213826 1105 80-81 0.695344717421964 1105 82-83 0.5963091271556245 1105 84-85 0.6727955375630863 1105 86-87 0.8053692846619995 1105 88-89 0.8251557112632639 1105 90-91 0.8963450010368383 1105 92-93 1.0519985023208989 1105 94-95 1.1412363109921344 1105 96-97 1.1091873927712363 1105 98-99 0.903049832849792 1105 100-101 1.4481045717346213 1106 1 0.12549046240767225 1106 2 0.15912855792013403 1106 3 0.2249265828759235 1106 4 0.154396999744435 1106 5 0.1045764962809912 1106 6 0.07569341922322081 1106 7 0.0993686977026158 1106 8 0.1137683410699637 1106 9 0.2235353242942395 1106 10-11 0.12315668569672766 1106 12-13 0.16973920722389835 1106 14-15 0.25053527136708453 1106 16-17 0.22128214470272667 1106 18-19 0.3066269773281718 1106 20-21 0.28057952273780273 1106 22-23 0.2763281636853705 1106 24-25 0.20085647865871437 1106 26-27 0.14956843455137658 1106 28-29 0.1595646037076719 1106 30-31 0.011058960444607635 1106 32-33 0.2022611854158498 1106 34-35 0.3181217878445324 1106 36-37 0.49175212647178057 1106 38-39 0.4744443796128692 1106 40-41 0.5379226012292904 1106 42-43 0.48245868421659566 1106 44-45 0.6820630182889005 1106 46-47 0.5442746317059317 1106 48-49 0.5123831406757873 1106 50-51 0.4373470266198254 1106 52-53 0.2603961340124741 1106 54-55 0.23996074610307971 1106 56-57 0.5371361106393593 1106 58-59 0.6085026868484533 1106 60-61 0.7766916376218589 1106 62-63 0.8120634549923196 1106 64-65 0.6672266953320758 1106 66-67 0.6477075125973002 1106 68-69 0.6860578583138732 1106 70-71 0.6652428189144537 1106 72-73 0.6932412524068177 1106 74-75 0.4940284386925349 1106 76-77 0.6000748292244786 1106 78-79 0.6701364570551505 1106 80-81 0.6877973872963388 1106 82-83 0.5153274264987608 1106 84-85 0.6623200667199818 1106 86-87 0.7129317912081667 1106 88-89 0.7111351033941844 1106 90-91 0.6793424937211618 1106 92-93 0.6851999147967724 1106 94-95 0.8858468113651341 1106 96-97 0.9725694153392155 1106 98-99 0.992753165890992 1106 100-101 1.477076839497979 1107 1 -0.046594475260697266 1107 2 0.04019105536211853 1107 3 -0.01574439909780523 1107 4 0.01797998492526176 1107 5 -0.01778927216794557 1107 6 -0.12569903339707622 1107 7 -0.12425514227000889 1107 8 0.014146350214808479 1107 9 0.03200736767399093 1107 10-11 -0.004864790092113935 1107 12-13 0.02604352242601493 1107 14-15 0.12877516235101183 1107 16-17 0.18629242399082813 1107 18-19 0.233942204632136 1107 20-21 0.18089771366705065 1107 22-23 0.17118597473746888 1107 24-25 -0.01924123492117502 1107 26-27 0.12013905310320894 1107 28-29 0.10006122083016322 1107 30-31 0.11348283127083647 1107 32-33 0.12068973465621013 1107 34-35 0.04941416563958967 1107 36-37 0.07505017230512578 1107 38-39 0.06873546554436416 1107 40-41 -0.03449692490549694 1107 42-43 -0.07625355028760339 1107 44-45 0.17240408181478983 1107 46-47 0.24297906885325204 1107 48-49 0.16394282193915188 1107 50-51 0.11188936479843647 1107 52-53 0.038263026938913924 1107 54-55 -0.13272549581228077 1107 56-57 0.023946718822706714 1107 58-59 0.14298696987466286 1107 60-61 0.18308019681094123 1107 62-63 0.1220978557134984 1107 64-65 0.07547106218774502 1107 66-67 0.00688218388571471 1107 68-69 0.15538567472862042 1107 70-71 0.16594686357032984 1107 72-73 0.10748947911156037 1107 74-75 0.18772296910380604 1107 76-77 0.04986242208700986 1107 78-79 0.01236250274577344 1107 80-81 -0.0433123875248107 1107 82-83 -0.004642412527271489 1107 84-85 0.13507391896059318 1107 86-87 0.0024690081079690174 1107 88-89 0.14748904672639185 1107 90-91 0.30250059116608696 1107 92-93 0.4622945493127233 1107 94-95 0.631044038318592 1107 96-97 0.5746523485726414 1107 98-99 0.6649905350648844 1107 100-101 0.8905971467327305 1108 1 0.09632795333655508 1108 2 0.11531222537296415 1108 3 0.09595796894268815 1108 4 0.16090382576039985 1108 5 0.17890806830566675 1108 6 0.15765039944818682 1108 7 0.1551708112730097 1108 8 0.09699754553295747 1108 9 0.14846502313314147 1108 10-11 0.18780710750369423 1108 12-13 0.3321065421295586 1108 14-15 0.31543911752093123 1108 16-17 0.4323832082912631 1108 18-19 0.3438157246068343 1108 20-21 0.30937238376827736 1108 22-23 0.2922408881955292 1108 24-25 0.2509304649613213 1108 26-27 0.2760030884882454 1108 28-29 0.3614757747889499 1108 30-31 0.4793922559816153 1108 32-33 0.5171369677997433 1108 34-35 0.6424216109577401 1108 36-37 0.7546478539754062 1108 38-39 0.6625853107630917 1108 40-41 0.7469160473468506 1108 42-43 0.7826936828559283 1108 44-45 0.8510261895515896 1108 46-47 0.7527969466551312 1108 48-49 0.7759784781213668 1108 50-51 0.7696640168230218 1108 52-53 0.8580271549667557 1108 54-55 0.9125634198149797 1108 56-57 0.8953956466516999 1108 58-59 0.8818163093992482 1108 60-61 0.8958764843199774 1108 62-63 0.8480474444698203 1108 64-65 0.8766810676107326 1108 66-67 0.8432731534717561 1108 68-69 0.8995928161331719 1108 70-71 0.8806846957286787 1108 72-73 1.0012538839380234 1108 74-75 1.0072402379487038 1108 76-77 1.1353464525009649 1108 78-79 1.0941185075812783 1108 80-81 1.0214274217665853 1108 82-83 1.170576201897891 1108 84-85 1.0781840339732476 1108 86-87 1.1534181731929287 1108 88-89 1.2894483290835836 1108 90-91 1.3349113094867633 1108 92-93 1.37527894690853 1108 94-95 1.454470099191692 1108 96-97 1.3956228102049621 1108 98-99 1.5914219793888158 1108 100-101 2.217145383130056 1109 1 -0.06069880510757031 1109 2 -0.02155741283220891 1109 3 -0.005680326062702079 1109 4 -0.007043729710311197 1109 5 0.032477311253920504 1109 6 -0.008115102034025767 1109 7 0.018208955811282124 1109 8 -0.011239546858348604 1109 9 -0.02679492566312547 1109 10-11 0.051349288827800876 1109 12-13 0.07435720463404749 1109 14-15 -0.004236142989306302 1109 16-17 0.09343082159075777 1109 18-19 -0.014689738248002016 1109 20-21 0.024509207789115806 1109 22-23 0.005425218841416779 1109 24-25 -0.0024434654869409655 1109 26-27 -0.05225855306314031 1109 28-29 0.0905264563825483 1109 30-31 0.02184658247281135 1109 32-33 0.10909125779704709 1109 34-35 0.18743107129729708 1109 36-37 0.16280367727760137 1109 38-39 0.13045756249233875 1109 40-41 0.20333995477588473 1109 42-43 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-3.2450932416252947 2316 58-59 -3.0497002192574314 2316 60-61 -2.9090396833746723 2316 62-63 -3.112075262595223 2316 64-65 -3.4809011376119443 2316 66-67 -3.209737012040101 2316 68-69 -3.5385121457179913 2316 70-71 -3.436141466759306 2316 72-73 -3.356795043689342 2316 74-75 -3.395123725424412 2316 76-77 -3.3194683980076825 2316 78-79 -3.1974382475653123 2316 80-81 -3.264096799236299 2316 82-83 -3.273405344481475 2316 84-85 -3.3215773480958006 2316 86-87 -3.2979179745762153 2316 88-89 -3.516081248776075 2316 90-91 -3.8240479708921207 2316 92-93 -3.657335424486856 2316 94-95 -3.6322189252596075 2316 96-97 -3.917949374691702 2316 98-99 -4.356762196356133 2316 100-101 -4.23364193713989 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13982.0 3 3002.0 4 2597.0 5 2852.0 6 3121.0 7 3949.0 8 4922.0 9 5803.0 10 7434.0 11 9900.0 12 13192.0 13 16565.0 14 17980.0 15 21415.0 16 26536.0 17 33251.0 18 28596.0 19 19799.0 20 19640.0 21 21168.0 22 23388.0 23 26179.0 24 29617.0 25 33667.0 26 38513.0 27 44193.0 28 51573.0 29 59841.0 30 70317.0 31 83832.0 32 102095.0 33 126052.0 34 160381.0 35 220386.0 36 307123.0 37 468732.0 38 481569.0 39 79167.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.693723156130616 22.501840215121057 15.44812462546155 20.35631200328678 2 13.65310332259857 23.95003704389702 40.36952034128184 22.027339292222575 3 19.47710196679734 28.54536184327253 32.19190234555197 19.785633844378165 4 10.754134870027737 18.705497775168823 43.14443553217454 27.3959318226289 5 10.380832305090344 40.82622905242956 36.77209925241566 12.020839390064445 6 25.155162573323093 41.38029696786782 19.067889041951418 14.396651416857662 7 23.794383924944608 35.65736433934716 24.545954274238724 16.0022974614695 8 23.046459237733053 33.76273633982707 22.45602076083187 20.73478366160801 9 24.3219166369843 19.266916521241743 25.155375046950407 31.255791794823544 10-11 22.27305907210826 27.59800273753168 30.148879435040403 19.980058755319657 12-13 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27.96317373864478 32.95786081909911 19.01819342769957 38-39 19.111416215605345 29.785090306751634 32.44346601449645 18.660027463146562 40-41 18.794070960172505 29.762226058904734 32.73301159814036 18.7106913827824 42-43 18.28233608937477 30.052888155807146 31.03005053075251 20.63472522406557 44-45 19.137236425458536 28.885567423308416 31.201747037672046 20.775449113561002 46-47 20.34922722973156 29.867545145707826 30.73427911419822 19.04894851036239 48-49 19.61948782043976 29.55068950847734 30.980838699691503 19.8489839713914 50-51 17.985806195164255 29.85158674401483 32.33907705459648 19.82353000622443 52-53 19.101534684084893 30.962759044024967 30.247827010477323 19.687879261412817 54-55 18.433800887576457 29.345564410111596 31.08057708376023 21.140057618551715 56-57 19.719363642962932 29.468145852027085 30.265425663676414 20.54706484133357 58-59 20.692509905103293 28.73082248176941 30.39363783927153 20.183029773855765 60-61 19.544614737942744 29.614402308713377 31.650962297359786 19.190020655984092 62-63 18.9118243177501 29.309352485678403 31.796452944179077 19.98237025239242 64-65 18.623728062327285 30.502452347492888 30.830024729365803 20.043794860814028 66-67 19.145426624048064 29.232940017236125 29.75286900619737 21.868764352518443 68-69 18.435422362094826 29.274650486970806 30.86162047052005 21.428306680414316 70-71 19.99348565299309 29.117708447404606 30.20990985678732 20.678896042814984 72-73 19.46436483028505 27.81945289083778 31.685645548717957 21.03053673015921 74-75 18.362953435372827 30.718103425041104 30.046316815553286 20.872626324032787 76-77 18.929470959681733 28.713893874550706 30.45515458795353 21.901480577814034 78-79 18.927628226091915 28.543621604591348 31.020360665233305 21.508389504083432 80-81 19.66051194330427 28.747830229402076 30.260574550202367 21.331083277091285 82-83 19.159088017125878 28.24399883232659 31.340260780395063 21.25665237015247 84-85 18.649921789081088 28.580023177902657 30.87081060719331 21.899244425822946 86-87 18.610873988569963 29.040303014539592 30.784672482104785 21.564150514785656 88-89 19.00156745443603 28.373414206197356 30.14084259284211 22.484175746524503 90-91 19.191241005433568 27.26249038143157 30.87590881550336 22.670359797631498 92-93 20.07344814128847 28.228943321956105 30.725225524539333 20.97238301221609 94-95 19.300369107578817 27.333447645989178 30.951723993340412 22.41445925309159 96-97 18.386430481884048 27.15625814296882 31.64305268107716 22.814258694069974 98-99 19.210366169257416 27.82246538704382 29.950954176289045 23.016214267409712 100-101 19.149884593029025 26.293140068918564 31.173374692956074 23.383600645096337 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5674.0 1 3521.0 2 1404.5 3 1454.0 4 1281.5 5 1310.5 6 2089.5 7 3497.5 8 4891.5 9 5160.5 10 4310.0 11 3721.0 12 3820.5 13 4082.0 14 4206.5 15 4331.5 16 4587.0 17 4897.5 18 5228.0 19 5687.5 20 6540.5 21 7494.0 22 8586.0 23 10009.5 24 11581.0 25 14274.0 26 19865.0 27 26425.0 28 29629.0 29 34055.0 30 43427.0 31 52307.0 32 64013.5 33 80510.0 34 97911.0 35 111249.0 36 123856.0 37 135561.0 38 133374.0 39 126380.0 40 118569.5 41 108912.0 42 100820.5 43 97179.5 44 95714.5 45 91834.5 46 91632.5 47 112052.5 48 172087.0 49 165113.5 50 90492.5 51 51371.5 52 34458.5 53 26771.0 54 21868.5 55 17950.0 56 14958.0 57 12590.5 58 10199.0 59 8198.5 60 6791.5 61 5627.5 62 4382.0 63 3154.5 64 2333.5 65 1853.5 66 1354.5 67 1058.5 68 826.0 69 575.5 70 444.0 71 355.5 72 280.0 73 226.5 74 197.0 75 165.0 76 127.5 77 98.5 78 82.0 79 65.0 80 45.0 81 38.5 82 33.0 83 25.0 84 13.5 85 9.0 86 7.5 87 4.5 88 7.0 89 6.5 90 2.0 91 2.0 92 1.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27502147381983466 2 0.16522911372773583 3 0.191661298211708 4 0.16329050499266312 5 0.2330058575808549 6 0.1917731410233468 7 0.23632386099280625 8 0.24336995812605133 9 0.14830356823306254 10-11 0.20400128842919008 12-13 0.2095934290111306 14-15 0.17115678274459278 16-17 0.28175068298676975 18-19 0.22819661668038604 20-21 0.19484881834341408 22-23 0.24143134939097863 24-25 0.23022842775849112 26-27 0.33826858380158187 28-29 0.3095436216790141 30-31 0.2850500459301146 32-33 0.18975997041384823 34-35 0.3559397480405139 36-37 0.3932020447848443 38-39 0.3738345979027236 40-41 0.3362554131920833 42-43 0.3692304021569259 44-45 0.37860655786597947 46-47 0.3837513272013648 48-49 0.4038084714219248 50-51 0.39543890101762047 52-53 0.39568122710950454 54-55 0.38971627715543466 56-57 0.3681119740405378 58-59 0.3436929601660642 60-61 0.30915217183827826 62-63 0.37832695083688245 64-65 0.41076136621213744 66-67 0.4176210586593178 68-69 0.42254214237142546 70-71 0.4215914784724956 72-73 0.4129795819763072 74-75 0.4083008243560836 76-77 0.39172944776493324 78-79 0.3664716128031686 80-81 0.3767797919425456 82-83 0.3860813857771733 84-85 0.38751670185987136 86-87 0.38224144924424086 88-89 0.39100246948928097 90-91 0.41271861541581667 92-93 0.4124576488553261 94-95 0.4212373095689727 96-97 0.42202020925044437 98-99 0.4198765553607005 100-101 0.390965188552068 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2682336.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.429577960882103 #Duplication Level Percentage of deduplicated Percentage of total 1 71.69300987797233 17.51429974064216 2 11.654431454353913 5.69425683607791 3 4.821595729275059 3.5336864629254374 4 2.823629808111334 2.759203381197056 5 1.7524750101782365 2.1406112442823444 6 1.2129913135509711 1.7779719516159742 7 0.8584940510758042 1.4680853144796902 8 0.6639435854367047 1.2975889265642853 9 0.5076864870904111 1.1162309954455407 >10 3.1887343283621536 14.607563889954566 >50 0.38039585589967323 6.5539204766546995 >100 0.38668792408684355 19.613039226790576 >500 0.036768090487348734 6.19174112409531 >1k 0.015603265290849672 6.268467990517873 >5k 0.0021628288521969847 3.6880223250432387 >10k+ 0.0013903899764123471 5.77531011371333 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 39991 1.490901960082555 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 23859 0.8894858809634587 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 17722 0.6606927692876656 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14495 0.5403871849015186 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14419 0.5375538336733354 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11131 0.41497411211719937 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10675 0.3979740047481002 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10435 0.38902657981699534 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10299 0.38395637235603597 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9831 0.36650889374038154 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9047 0.3372806389654391 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8460 0.3153967288214452 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8294 0.3092080932440977 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8243 0.3073067654462379 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7518 0.28027808596685877 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6808 0.25380862054567366 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6387 0.23811334597902722 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6311 0.23527999475084402 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5712 0.21294871336029492 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5412 0.20176443219641388 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5382 0.20064600408002575 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5203 0.19397271631891008 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5112 0.19058015103253284 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4566 0.17022475931426934 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4494 0.1675405318349379 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4050 0.15098779571239399 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3662 0.13652279207377452 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3478 0.1296630996265941 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3330 0.12414552091907949 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3273 0.12202050749794209 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3039 0.11329676819011487 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2925 0.10904674134784009 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.7280937212936784E-5 0.0 5 0.0 0.0 0.0 3.7280937212936784E-5 0.0 6 0.0 0.0 0.0 3.7280937212936784E-5 0.0 7 0.0 0.0 0.0 3.7280937212936784E-5 0.0 8 0.0 0.0 0.0 3.7280937212936784E-5 0.0 9 0.0 0.0 0.0 3.7280937212936784E-5 0.0 10-11 0.0 0.0 0.0 5.5921405819405175E-5 0.0 12-13 3.7280937212936784E-5 0.0 0.0 7.456187442587357E-5 0.0 14-15 3.7280937212936784E-5 0.0 0.0 9.320234303234196E-5 0.0 16-17 3.7280937212936784E-5 0.0 0.0 2.236856232776207E-4 0.0 18-19 3.7280937212936784E-5 0.0 0.0 3.168879663099627E-4 0.0 20-21 3.7280937212936784E-5 0.0 0.0 7.64259212865204E-4 0.0 22-23 3.7280937212936784E-5 0.0 0.0 0.001994530140892118 0.0 24-25 3.7280937212936784E-5 0.0 0.0 0.004604195745797693 0.0 26-27 3.7280937212936784E-5 0.0 0.0 0.008313648998484902 0.0 28-29 3.7280937212936784E-5 0.0 0.0 0.020802762964818727 0.0 30-31 3.7280937212936784E-5 0.0 0.0 0.055175787075146435 0.0 32-33 3.7280937212936784E-5 0.0 0.0 0.12764992901709554 0.0 34-35 3.7280937212936784E-5 0.0 0.0 0.23032163010152346 0.0 36-37 3.7280937212936784E-5 0.0 0.0 0.37651882538205506 0.0 38-39 3.7280937212936784E-5 0.0 0.0 0.5848447025279457 0.0 40-41 3.7280937212936784E-5 0.0 0.0 0.815949232310941 0.0 42-43 3.7280937212936784E-5 0.0 0.0 1.0500921584767904 0.0 44-45 3.7280937212936784E-5 0.0 0.0 1.3295500638249647 0.0 46-47 3.7280937212936784E-5 0.0 0.0 1.6672594335683524 0.0 48-49 3.7280937212936784E-5 0.0 0.0 2.0037012514465005 0.0 50-51 3.7280937212936784E-5 0.0 0.0 2.3694458859740166 0.0 52-53 3.7280937212936784E-5 0.0 0.0 2.714219993319256 0.0 54-55 3.7280937212936784E-5 0.0 0.0 3.088520602937141 0.0 56-57 3.7280937212936784E-5 0.0 0.0 3.5604786275843145 0.0 58-59 3.7280937212936784E-5 0.0 0.0 4.037879669064576 0.0 60-61 3.7280937212936784E-5 0.0 0.0 4.567604505923195 0.0 62-63 3.7280937212936784E-5 0.0 0.0 5.079975066509192 0.0 64-65 3.7280937212936784E-5 0.0 0.0 5.598310577049258 0.0 66-67 3.7280937212936784E-5 0.0 0.0 6.195197022296982 0.0 68-69 3.7280937212936784E-5 0.0 0.0 6.858760423750045 0.0 70-71 3.7280937212936784E-5 0.0 0.0 7.570528077019434 0.0 72-73 3.7280937212936784E-5 0.0 0.0 8.25275058754757 0.0 74-75 3.7280937212936784E-5 0.0 0.0 8.993261843408133 0.0 76-77 3.7280937212936784E-5 0.0 0.0 9.726671080729632 0.0 78-79 3.7280937212936784E-5 0.0 0.0 10.619773212602745 0.0 80-81 3.7280937212936784E-5 0.0 0.0 11.559830684895553 0.0 82-83 3.7280937212936784E-5 0.0 0.0 12.423424954964627 0.0 84-85 3.7280937212936784E-5 0.0 0.0 13.322305632105746 0.0 86-87 3.7280937212936784E-5 0.0 0.0 14.271366450735478 0.0 88-89 3.7280937212936784E-5 0.0 0.0 15.24814564618303 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 620 0.0 35.963173 9 GATCGTG 120 1.5224214E-7 31.63014 7 GTAAGCG 340 0.0 29.347183 94-95 ACGAGTG 65 0.005876887 29.198692 5 AGTTCGC 65 0.0058784974 29.197052 8 GTATGGT 505 0.0 27.295769 1 CATGGGG 3790 0.0 25.664417 4 TAGGCAT 985 0.0 25.048622 5 TCGTGCA 95 0.0012359358 24.968832 9 CATATAG 830 0.0 24.581429 3 GTACATG 13335 0.0 24.202791 1 TAGGACG 295 0.0 24.126038 4 GAGTACG 220 2.1555024E-9 23.766144 1 ACATGGG 13350 0.0 22.675463 3 TACATGG 14215 0.0 22.363752 2 GAGTACT 11290 0.0 22.305462 12-13 TTAGGCA 1215 0.0 22.259495 4 AGTACTT 12295 0.0 20.887598 12-13 ATGGGAT 3045 0.0 20.88018 5 GTACTTT 12025 0.0 20.765615 14-15 >>END_MODULE