##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938768_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3492315 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.379626980956758 31.0 31.0 34.0 30.0 34.0 2 31.396401527353632 33.0 31.0 34.0 28.0 34.0 3 31.413131690583466 33.0 31.0 34.0 28.0 34.0 4 34.95828726790109 37.0 35.0 37.0 32.0 37.0 5 35.009222822110836 37.0 35.0 37.0 32.0 37.0 6 35.09255207505623 37.0 35.0 37.0 32.0 37.0 7 35.00422355944409 37.0 35.0 37.0 32.0 37.0 8 34.988919957105814 37.0 35.0 37.0 32.0 37.0 9 36.47902437208557 39.0 37.0 39.0 32.0 39.0 10-11 36.350338958541826 39.0 37.0 39.0 32.0 39.0 12-13 36.245374486551185 39.0 37.0 39.0 32.0 39.0 14-15 37.25478801310878 40.0 37.0 41.0 31.5 41.0 16-17 37.16375684896695 40.0 37.0 41.0 31.0 41.0 18-19 37.19392952812103 40.0 37.0 41.0 31.0 41.0 20-21 37.10030882093969 40.0 36.5 41.0 31.0 41.0 22-23 37.12435375961218 40.0 37.0 41.0 31.0 41.0 24-25 37.049158938984604 40.0 36.5 41.0 31.0 41.0 26-27 36.84951514969297 39.5 36.0 41.0 30.0 41.0 28-29 36.6646133009193 39.0 36.0 41.0 30.0 41.0 30-31 36.44716341452589 39.0 35.5 41.0 30.0 41.0 32-33 36.232659711394874 39.0 35.0 41.0 30.0 41.0 34-35 35.9269740845256 39.0 35.0 41.0 28.5 41.0 36-37 35.66354495513721 38.5 35.0 40.0 27.0 41.0 38-39 35.43643657573844 38.0 35.0 40.0 26.5 41.0 40-41 35.297405159614755 38.0 35.0 40.0 26.0 41.0 42-43 35.28681061129938 39.0 35.0 40.5 25.5 41.0 44-45 35.058350979221515 39.0 35.0 41.0 24.5 41.0 46-47 34.650136657203035 38.0 34.0 40.0 23.5 41.0 48-49 34.30775273708127 38.0 34.0 40.0 21.5 41.0 50-51 33.15084807641922 37.5 32.0 39.5 17.5 40.5 52-53 33.1949537770791 37.5 33.0 39.5 18.0 40.5 54-55 33.7180219997337 38.0 33.0 40.0 18.0 41.0 56-57 33.64469442189493 38.0 33.0 40.0 16.5 41.0 58-59 33.4653616583842 38.0 33.0 40.0 14.5 41.0 60-61 33.10848663422401 37.5 32.5 40.0 12.5 41.0 62-63 32.673819085620856 37.0 32.0 40.0 8.0 41.0 64-65 32.222711582431714 36.0 31.0 39.5 7.5 41.0 66-67 31.797926733413224 36.0 31.0 39.0 4.0 41.0 68-69 31.371764431329936 35.5 31.0 39.0 2.0 40.0 70-71 30.87431660660622 35.0 30.0 38.0 2.0 40.0 72-73 30.375516813345875 35.0 29.5 37.0 2.0 39.5 74-75 29.87564881747494 35.0 29.0 37.0 2.0 39.0 76-77 29.349081626371046 34.0 29.0 36.0 2.0 39.0 78-79 28.873774702453815 34.0 28.5 36.0 2.0 38.5 80-81 28.37810836078647 34.0 27.5 35.0 2.0 37.0 82-83 27.928881272164737 34.0 26.5 35.0 2.0 37.0 84-85 27.537898070477606 34.0 26.0 35.0 2.0 36.0 86-87 27.05079596199083 34.0 25.5 35.0 2.0 36.0 88-89 26.493814990915766 33.0 24.5 35.0 2.0 35.5 90-91 25.93506384733336 33.0 22.5 35.0 2.0 35.0 92-93 25.41355003772569 32.5 19.5 35.0 2.0 35.0 94-95 24.859002552747963 32.0 15.5 35.0 2.0 35.0 96-97 24.157194296619863 32.0 2.0 35.0 2.0 35.0 98-99 23.304064209557268 31.5 2.0 34.0 2.0 35.0 100-101 20.895467190101694 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2895851928058022 1101 2 0.37219415517743926 1101 3 0.45223012623159065 1101 4 0.2448136070419551 1101 5 0.2301671797348419 1101 6 0.3982875250491489 1101 7 0.349139986506124 1101 8 0.42551429235959404 1101 9 0.5882816787035736 1101 10-11 0.3882493454179965 1101 12-13 0.5146616839004992 1101 14-15 0.584047796813536 1101 16-17 0.6184457518650248 1101 18-19 0.6699948625130574 1101 20-21 0.827105282754566 1101 22-23 0.6313758880907017 1101 24-25 0.6568110558692055 1101 26-27 0.6732357527660966 1101 28-29 0.6730744157027289 1101 30-31 0.8199482908856766 1101 32-33 0.796053212062624 1101 34-35 0.7904340229189017 1101 36-37 0.863791749842818 1101 38-39 0.8318299744223339 1101 40-41 1.0094060301367165 1101 42-43 1.160413823651652 1101 44-45 1.1549853345787824 1101 46-47 1.2277806359053187 1101 48-49 1.1992529190512755 1101 50-51 1.111715259809671 1101 52-53 1.1982216546249376 1101 54-55 1.2783754878422044 1101 56-57 1.2516162485657603 1101 58-59 1.0909816760618156 1101 60-61 1.062655150404602 1101 62-63 0.9851747772917534 1101 64-65 0.9285645739655166 1101 66-67 0.9105489686359007 1101 68-69 1.0391807448295793 1101 70-71 0.9075519188951091 1101 72-73 0.9978805088329423 1101 74-75 0.9452060384236951 1101 76-77 0.9231798187685456 1101 78-79 0.9052982772260663 1101 80-81 0.8682454682056466 1101 82-83 1.0604775249539635 1101 84-85 1.0256503821982257 1101 86-87 0.9104090123590005 1101 88-89 0.9589690745769914 1101 90-91 0.9731675407344866 1101 92-93 0.8202634515920657 1101 94-95 0.7283372615039347 1101 96-97 0.7438409714883569 1101 98-99 0.9065114367311722 1101 100-101 1.1326434916354238 1102 1 0.3358247049721612 1102 2 0.37424564347642075 1102 3 0.37554892499658266 1102 4 0.4848547578016209 1102 5 0.504570699005626 1102 6 0.5453092936687298 1102 7 0.43286382696513215 1102 8 0.5004176930162316 1102 9 0.6637565728764443 1102 10-11 0.6970567340346392 1102 12-13 0.63123240660628 1102 14-15 0.7388478059445234 1102 16-17 0.9141349645760428 1102 18-19 0.8761528200944397 1102 20-21 0.7782667051918466 1102 22-23 0.790865209228194 1102 24-25 0.7499752674529603 1102 26-27 0.7762505444056913 1102 28-29 0.8759484432865392 1102 30-31 0.9842961135357129 1102 32-33 0.9056168676884511 1102 34-35 1.1252160540012142 1102 36-37 1.1993456399874987 1102 38-39 1.0329300328945479 1102 40-41 1.1319754182014705 1102 42-43 1.23094213290765 1102 44-45 1.1272283341058653 1102 46-47 1.1715726151692607 1102 48-49 1.203521253688514 1102 50-51 1.200918339728176 1102 52-53 1.1148339346291678 1102 54-55 1.1644787659564386 1102 56-57 1.1121816310941597 1102 58-59 1.0986896999033817 1102 60-61 1.0494162227990742 1102 62-63 0.9477196891401931 1102 64-65 1.0361213879629716 1102 66-67 1.0774366907692183 1102 68-69 1.1454191712792436 1102 70-71 1.047136608870165 1102 72-73 0.9890037263035261 1102 74-75 0.9039198464894724 1102 76-77 0.8185897699830811 1102 78-79 0.772308590163334 1102 80-81 0.7840627390220227 1102 82-83 0.686302046518712 1102 84-85 0.691702763914595 1102 86-87 0.6413121552184258 1102 88-89 0.7096235899283059 1102 90-91 0.5710796252693875 1102 92-93 0.5808919605602405 1102 94-95 0.7805990327982748 1102 96-97 0.7586797179582625 1102 98-99 0.4162174696619445 1102 100-101 0.6729665334876991 1103 1 0.014438671410268 1103 2 0.22466610450370084 1103 3 0.3379668650925538 1103 4 0.30041680183016695 1103 5 0.3003338591047111 1103 6 0.22976950722990352 1103 7 0.18678120264372922 1103 8 0.21323080505008818 1103 9 0.3085855125296604 1103 10-11 0.30079943948855004 1103 12-13 0.36803967901444423 1103 14-15 0.47194715536873844 1103 16-17 0.523643861628706 1103 18-19 0.5655625551972108 1103 20-21 0.4382459633519602 1103 22-23 0.46379299970370624 1103 24-25 0.4557272588690324 1103 26-27 0.48873665335619165 1103 28-29 0.5663290560483603 1103 30-31 0.7097817318619235 1103 32-33 0.6055729508313235 1103 34-35 0.5477509062754393 1103 36-37 0.6330141534756706 1103 38-39 0.7509748751568424 1103 40-41 0.7554399436720445 1103 42-43 0.7919755551915628 1103 44-45 0.8204023940418068 1103 46-47 0.7973531665033207 1103 48-49 0.7738133147493045 1103 50-51 0.8847443715349925 1103 52-53 0.8134436330348436 1103 54-55 0.800683886797593 1103 56-57 0.8102796043770724 1103 58-59 0.8454952571209375 1103 60-61 0.7621534675647581 1103 62-63 0.6383240345556445 1103 64-65 0.5476496626806622 1103 66-67 0.5742946211324806 1103 68-69 0.5743073288853182 1103 70-71 0.5944683706102438 1103 72-73 0.6427268067858556 1103 74-75 0.49260772567623334 1103 76-77 0.5046376916108386 1103 78-79 0.49843381503279716 1103 80-81 0.550432518161319 1103 82-83 0.5037404691815652 1103 84-85 0.4267054164768105 1103 86-87 0.40269691235940286 1103 88-89 0.44223502745747467 1103 90-91 0.39172916396288926 1103 92-93 0.2502126254159869 1103 94-95 0.3389558004218607 1103 96-97 0.2777061677929247 1103 98-99 0.4877171844616477 1103 100-101 0.9486483616415562 1104 1 0.0985556811610806 1104 2 0.23585294583138605 1104 3 0.2388493628764614 1104 4 0.3947081442905258 1104 5 0.32240221328574137 1104 6 0.38498077483553317 1104 7 0.39652807548919355 1104 8 0.39831107689092704 1104 9 0.526241377791159 1104 10-11 0.3287182812102074 1104 12-13 0.4583696307528484 1104 14-15 0.5591292690297252 1104 16-17 0.656781850400499 1104 18-19 0.567007448332248 1104 20-21 0.5565774909792651 1104 22-23 0.5057539964534286 1104 24-25 0.42505750608660264 1104 26-27 0.43367903491829196 1104 28-29 0.5073720975139366 1104 30-31 0.6191927433856108 1104 32-33 0.5052508785374172 1104 34-35 0.6456421699413468 1104 36-37 0.5584184674715331 1104 38-39 0.5213599830067466 1104 40-41 0.6229825419188657 1104 42-43 0.6585154941258651 1104 44-45 0.6763750129721728 1104 46-47 0.7478909385903805 1104 48-49 0.8058343922633426 1104 50-51 0.9299595788628778 1104 52-53 0.8309448641980453 1104 54-55 0.7790351130361515 1104 56-57 0.8983855829055827 1104 58-59 0.8105046139612853 1104 60-61 0.796756048082834 1104 62-63 0.9124439990981514 1104 64-65 0.912929875425661 1104 66-67 0.7784923951894172 1104 68-69 0.7487217876659713 1104 70-71 0.8339562067178008 1104 72-73 0.6540761616563344 1104 74-75 0.6121428384507794 1104 76-77 0.67798153087017 1104 78-79 0.47355643888781884 1104 80-81 0.5000503651031103 1104 82-83 0.5334331708460986 1104 84-85 0.6963242483491676 1104 86-87 0.8070581848896872 1104 88-89 0.8666041794322616 1104 90-91 0.6475530583783318 1104 92-93 0.7102756609719769 1104 94-95 0.8686494870169561 1104 96-97 0.7810342481632624 1104 98-99 0.8839577045049865 1104 100-101 1.191641565620678 1105 1 0.02042746207477819 1105 2 0.11895164354086418 1105 3 0.12458865352592952 1105 4 0.16724698269762683 1105 5 0.15493267683417145 1105 6 0.24167103068747053 1105 7 0.2516781948309301 1105 8 0.21691163800645796 1105 9 0.2422800696382339 1105 10-11 0.24097823682345165 1105 12-13 0.22480905958748565 1105 14-15 0.36582664351152516 1105 16-17 0.3736827339480868 1105 18-19 0.4324925620209541 1105 20-21 0.4140855517807438 1105 22-23 0.3785051375279238 1105 24-25 0.37170546890222766 1105 26-27 0.3676692363336471 1105 28-29 0.4944442469214181 1105 30-31 0.43239905802906264 1105 32-33 0.5876385507454458 1105 34-35 0.514900576205747 1105 36-37 0.6453792312586444 1105 38-39 0.6613855263257022 1105 40-41 0.7011136922057091 1105 42-43 0.6047154020024195 1105 44-45 0.6279490676599977 1105 46-47 0.7543090115354047 1105 48-49 0.7770788962623314 1105 50-51 0.7581238098414786 1105 52-53 0.7441381978105355 1105 54-55 0.6013327555134325 1105 56-57 0.4604960472914996 1105 58-59 0.506868669067849 1105 60-61 0.6494514963471474 1105 62-63 0.5476150555865686 1105 64-65 0.45134828336502864 1105 66-67 0.4768354294007082 1105 68-69 0.4254606176982172 1105 70-71 0.46091864547664585 1105 72-73 0.6145304977394126 1105 74-75 0.5613547642495362 1105 76-77 0.5367261395549008 1105 78-79 0.5544257492182041 1105 80-81 0.6844454909353566 1105 82-83 0.6255997467851309 1105 84-85 0.7206414879957457 1105 86-87 0.6155260044952335 1105 88-89 0.620257292785972 1105 90-91 0.7275237463729738 1105 92-93 0.7962980218446383 1105 94-95 0.9202046982727659 1105 96-97 0.9468551936848044 1105 98-99 0.8249836246925817 1105 100-101 1.0670140374602148 1106 1 0.0325174178241987 1106 2 0.10079341897364813 1106 3 0.10091479405499015 1106 4 0.17867600718434318 1106 5 0.1887475088906001 1106 6 0.11653488386726707 1106 7 0.032930100369661375 1106 8 0.08406009868551223 1106 9 0.16376751858825145 1106 10-11 0.22025645824879092 1106 12-13 0.13619104448964237 1106 14-15 0.31696608369323087 1106 16-17 0.36021189472557325 1106 18-19 0.29299594315719446 1106 20-21 0.3731450568552148 1106 22-23 0.22928198460306248 1106 24-25 0.11099410813385902 1106 26-27 0.04233204179750061 1106 28-29 0.13173025630659652 1106 30-31 0.04979559862638894 1106 32-33 0.04084560656502134 1106 34-35 0.09420811985798849 1106 36-37 0.3309192024295555 1106 38-39 0.3718196399757545 1106 40-41 0.5179425317527446 1106 42-43 0.5702896163963302 1106 44-45 0.47839522763680975 1106 46-47 0.5842925462043596 1106 48-49 0.5585773314505431 1106 50-51 0.5306166597803355 1106 52-53 0.3242618103384274 1106 54-55 0.3799784008305309 1106 56-57 0.5472972950068851 1106 58-59 0.5403469820623954 1106 60-61 0.6186978545811925 1106 62-63 0.5913493194355297 1106 64-65 0.41331948852479883 1106 66-67 0.49556898824647533 1106 68-69 0.5729277959240555 1106 70-71 0.6523545103398618 1106 72-73 0.6473173170293052 1106 74-75 0.44651398940452225 1106 76-77 0.6372818887710139 1106 78-79 0.6072071602846734 1106 80-81 0.5790691439916991 1106 82-83 0.5647140841286387 1106 84-85 0.7987212925851033 1106 86-87 0.8040372134553735 1106 88-89 0.8543956068819476 1106 90-91 0.8798132202398001 1106 92-93 0.987623780712287 1106 94-95 1.1755354082560068 1106 96-97 1.148582333384251 1106 98-99 1.2832116320257967 1106 100-101 1.7071348978918799 1107 1 -0.0766850493314486 1107 2 -0.08606384241633691 1107 3 0.01103731880542469 1107 4 0.002772741563752845 1107 5 -0.10192137679324276 1107 6 -0.08367445482689106 1107 7 -0.03989190740560389 1107 8 -0.07859690721130619 1107 9 -0.07821402292324109 1107 10-11 -0.07267227068394533 1107 12-13 -0.039641638553383984 1107 14-15 0.048203576017698424 1107 16-17 0.10392025156767914 1107 18-19 0.06279655131559281 1107 20-21 -0.004130865412548701 1107 22-23 0.0778292593200618 1107 24-25 -0.0667269684316878 1107 26-27 0.0516565969246372 1107 28-29 0.052755706710492234 1107 30-31 -0.08356370879529607 1107 32-33 0.015803275780463366 1107 34-35 0.10735268963748013 1107 36-37 0.1673418462510341 1107 38-39 0.09027883393997627 1107 40-41 -0.011358656451101012 1107 42-43 -0.005812623799378969 1107 44-45 0.16057433882616579 1107 46-47 0.13768014219254354 1107 48-49 0.008527454596688244 1107 50-51 0.14297613065640746 1107 52-53 0.09529511826053039 1107 54-55 0.09216989192632496 1107 56-57 0.17896967507241612 1107 58-59 0.25944258246759233 1107 60-61 0.26302309039304106 1107 62-63 0.11540923530079539 1107 64-65 0.1772801294881461 1107 66-67 -0.036685448549583555 1107 68-69 0.1766485371754598 1107 70-71 0.2755510147874283 1107 72-73 0.12602212935769685 1107 74-75 0.13366067313408436 1107 76-77 0.043985455003589635 1107 78-79 -0.05287712754574869 1107 80-81 -0.12844337137623896 1107 82-83 -0.32056843905479226 1107 84-85 -0.08796211277172716 1107 86-87 -0.19857194038072024 1107 88-89 -0.23018708685540545 1107 90-91 0.07798823471279093 1107 92-93 0.2042184088715615 1107 94-95 0.1705929392481771 1107 96-97 0.25025696788957674 1107 98-99 0.47116301717792197 1107 100-101 0.8160235146069432 1108 1 0.28295954991277483 1108 2 0.3232210771125601 1108 3 0.19900541129337768 1108 4 0.2585088559173201 1108 5 0.27076185599320723 1108 6 0.27542730832554696 1108 7 0.1832867222478427 1108 8 0.33578488392994643 1108 9 0.3203660193071016 1108 10-11 0.32855220756587045 1108 12-13 0.4524789468186867 1108 14-15 0.49148647116953725 1108 16-17 0.6077757859596531 1108 18-19 0.5484983722144747 1108 20-21 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-2.936281415114795 2316 88-89 -3.155947913050351 2316 90-91 -3.4172176973224033 2316 92-93 -3.2793622799219726 2316 94-95 -3.238379886602477 2316 96-97 -3.5053254263685147 2316 98-99 -3.759337941591305 2316 100-101 -3.6160011817975892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18185.0 3 4481.0 4 4277.0 5 4830.0 6 5557.0 7 6895.0 8 8467.0 9 10113.0 10 12313.0 11 15714.0 12 20128.0 13 24924.0 14 26411.0 15 30830.0 16 37105.0 17 45551.0 18 39795.0 19 29142.0 20 29246.0 21 31430.0 22 34369.0 23 37919.0 24 43119.0 25 48563.0 26 55513.0 27 64065.0 28 73609.0 29 85194.0 30 100196.0 31 118789.0 32 143572.0 33 176925.0 34 227799.0 35 310550.0 36 424910.0 37 579886.0 38 489135.0 39 72796.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.36394514170081 18.926555375535074 13.745037593272574 20.96446188949154 2 14.128335745910976 22.191947487931614 40.39693235996557 23.28278440619184 3 21.27246145468921 28.03326105579524 29.46338661673989 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48-49 21.71595628256623 27.484571404092435 28.899210956930173 21.900261356411164 50-51 21.806388014545423 28.076491965031884 29.21512049936711 20.901999521055586 52-53 20.897496784520623 28.604642939485743 29.197415197301357 21.30044507869228 54-55 19.889007962128577 27.80403585458781 29.893066928678895 22.41388925460471 56-57 21.38805382475341 28.57298875681144 28.106117651116268 21.932839767318878 58-59 21.481249064385505 27.493547312857707 28.686479138634148 22.33872448412264 60-61 21.145853888463307 27.173845918314882 29.68007098108253 22.00022921213928 62-63 22.237615628996156 26.893760295338797 29.244280814446626 21.624343261218414 64-65 21.369525701177768 27.592056088470912 29.704603365101235 21.33381484525008 66-67 20.768384179150186 27.537080341398646 29.37736368531113 22.317171794140037 68-69 20.430756073974365 28.4730084084262 29.073267266374376 22.022968251225063 70-71 20.844919475473418 28.226602798387614 28.195474776381467 22.733002949757502 72-73 20.93940044368875 27.242592739819006 28.317598450899162 23.50040836559309 74-75 20.865785618686306 28.139793649036605 28.475843538989466 22.51857719328763 76-77 21.48943989979982 26.79939395794875 29.123451099302844 22.58771504294858 78-79 20.922197490167452 26.858771984026507 29.767683139747923 22.451347386058114 80-81 20.664572183848282 28.254852024515824 28.909783896338766 22.17079189529713 82-83 20.450951283078382 27.801183827246252 28.56925007074994 23.178614818925425 84-85 20.596294224479458 27.012052480376774 28.523053731962815 23.868599563180947 86-87 21.126419478916336 26.96291695093493 28.88738492794473 23.023278642204005 88-89 21.647109519306547 25.989438723597345 29.14938446694071 23.214067290155395 90-91 20.83756243009411 26.22823033505948 29.780973521301707 23.153233713544708 92-93 20.55910204161436 27.390097109375855 29.275153007503878 22.775647841505904 94-95 20.34123716083321 27.285716750439953 28.78462117988061 23.58842490884623 96-97 20.1657286190269 26.544888692330055 28.62311016205656 24.666272526586482 98-99 20.99155749610986 26.262741716832714 28.247271436973943 24.49842935008348 100-101 21.558304022532774 25.685560832002725 29.0771190627213 23.6790160827432 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5352.0 1 3609.5 2 2031.5 3 2251.5 4 2028.0 5 2022.0 6 3245.5 7 5847.5 8 8449.0 9 8861.5 10 7341.0 11 6163.5 12 6200.5 13 6605.0 14 6864.0 15 7058.0 16 7221.5 17 7425.5 18 7757.0 19 8229.5 20 9014.5 21 10009.5 22 11262.5 23 12897.0 24 14306.5 25 16517.0 26 20952.5 27 25812.0 28 29667.0 29 34053.0 30 40124.0 31 46944.0 32 56007.0 33 66455.5 34 77062.0 35 84680.0 36 93840.0 37 104162.5 38 104988.0 39 103014.5 40 101601.5 41 101315.5 42 101130.5 43 102565.0 44 108413.5 45 112592.0 46 121296.0 47 148114.0 48 248484.0 49 253683.5 50 164794.0 51 129659.0 52 96855.5 53 83184.5 54 77138.5 55 70526.0 56 62648.0 57 54909.0 58 49246.5 59 44165.0 60 38641.0 61 32655.0 62 26708.5 63 20255.5 64 14630.0 65 11330.0 66 8770.0 67 7051.0 68 5659.0 69 4167.5 70 3126.0 71 2518.0 72 2135.0 73 1877.5 74 1582.5 75 1294.0 76 1013.0 77 736.0 78 528.0 79 414.0 80 313.0 81 213.0 82 169.5 83 151.0 84 114.0 85 71.5 86 56.0 87 41.0 88 29.5 89 23.0 90 15.0 91 12.0 92 12.5 93 7.5 94 1.5 95 3.5 96 4.5 97 2.0 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26853247774041 2 0.16396000933478222 3 0.19119122988619297 4 0.16249965996767188 5 0.2334840929297615 6 0.19239387054146032 7 0.23772196952451313 8 0.2449664477574331 9 0.1476670918860412 10-11 0.20348966230136745 12-13 0.20887291094875463 14-15 0.17134765907428168 16-17 0.28399500045099024 18-19 0.22977595090935382 20-21 0.19676060149213342 22-23 0.24273297225479376 24-25 0.23140810608435952 26-27 0.33953122785315754 28-29 0.31154119831687577 30-31 0.28770314247139794 32-33 0.19016039503882096 34-35 0.35598163395913596 36-37 0.39419410906519026 38-39 0.3742646353493313 40-41 0.33704004363867524 42-43 0.37006971020655355 44-45 0.3787029520532942 46-47 0.38372827193423276 48-49 0.4041588459231198 50-51 0.39606965580138104 52-53 0.39598375289743337 54-55 0.389340594992147 56-57 0.36866662944207496 58-59 0.3430961983669858 60-61 0.309937677443186 62-63 0.37774370295921184 64-65 0.4117898872238042 66-67 0.4169870129126381 68-69 0.4218405269856815 70-71 0.4220982356975244 72-73 0.41309274793367723 74-75 0.4083251367645817 76-77 0.391674290549392 78-79 0.36651905684338326 80-81 0.3772426026861838 82-83 0.3869496308322703 84-85 0.3873934625026666 86-87 0.382368142621728 88-89 0.3912447760296537 90-91 0.41304979648170337 92-93 0.4117898872238042 94-95 0.4202083718106757 96-97 0.4209814979462047 98-99 0.4184759965810644 100-101 0.39081526150991536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3492315.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.15224899663201 #Duplication Level Percentage of deduplicated Percentage of total 1 68.02900534802484 23.913725349869804 2 13.896183993359394 9.769642396751632 3 6.235127448785279 6.575362578163051 4 3.3555430393581034 4.718195377537256 5 2.079218294933718 3.654459961093136 6 1.386730461450649 2.924801668327657 7 0.9468388613806885 2.3298460790459057 8 0.690190003521081 1.9409384687007492 9 0.5325218018189787 1.684740507630828 >10 2.552321698284722 15.401263670194727 >50 0.16840669303343872 4.148455214621664 >100 0.11131942683141215 7.400801673925411 >500 0.008542894250860905 2.043785743044547 >1k 0.005996454618178836 4.47637588028849 >5k 9.857185673718635E-4 2.353674250953558 >10k+ 0.0010678617813195189 6.6639311798516285 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 37242 1.06639864960635 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 31356 0.8978571520610255 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22163 0.6346220200640549 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21380 0.6122013621337136 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 20852 0.597082451038924 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14587 0.4176885532948775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14564 0.41702996436461204 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 13435 0.38470183817897297 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 12255 0.3509133626262236 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11150 0.3192724596721659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11075 0.3171248870734742 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 10705 0.3065301955865951 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 10020 0.2869156991852109 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9656 0.27649281350622723 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 9449 0.27056551313383814 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8481 0.2428475094600573 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8238 0.23588937424029618 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6626 0.18973088051908263 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6064 0.1736384031795528 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5901 0.16897101206506288 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5753 0.16473313547031126 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5657 0.16198424254398588 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5542 0.1586912978926586 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5117 0.14652171983340564 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5028 0.14397326701629148 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4835 0.13844684686232486 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4767 0.1364997143728444 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4505 0.12899752742808138 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4295 0.12298432415174462 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4089 0.11708565808067142 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4055 0.11611209183593119 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3798 0.10875307639774763 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3786 0.10840946478195695 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3785 0.10838083048064107 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3607 0.10328392484641277 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3543 0.10145132956219584 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3542 0.10142269526087996 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.7268602631778634E-5 0.0 10-11 0.0 0.0 0.0 7.158575328972328E-5 0.0 12-13 0.0 0.0 0.0 1.002200546056126E-4 0.0 14-15 0.0 0.0 0.0 1.431715065794466E-4 0.0 16-17 0.0 0.0 2.8634301315889317E-5 3.865630677645058E-4 0.0 18-19 0.0 0.0 2.8634301315889317E-5 6.872232315813436E-4 0.0 20-21 0.0 0.0 2.8634301315889317E-5 0.0011453720526355727 0.0 22-23 0.0 0.0 2.8634301315889317E-5 0.002390964159876758 0.0 24-25 0.0 0.0 5.7268602631778634E-5 0.004151973690803951 0.0 26-27 0.0 0.0 8.590290394766794E-5 0.006829280863839602 0.0 28-29 0.0 0.0 8.590290394766794E-5 0.01404512479544371 0.0 30-31 0.0 0.0 8.590290394766794E-5 0.034289575825777456 0.0 32-33 0.0 0.0 8.590290394766794E-5 0.07436328051736456 0.0 34-35 0.0 0.0 8.590290394766794E-5 0.14348648389392138 0.0 36-37 0.0 0.0 8.590290394766794E-5 0.252754977715355 0.0 38-39 0.0 0.0 8.590290394766794E-5 0.40361479419811785 1.4317150657944659E-5 40-41 0.0 0.0 8.590290394766794E-5 0.5834525236125607 2.8634301315889317E-5 42-43 0.0 0.0 8.590290394766794E-5 0.7545711082763153 2.8634301315889317E-5 44-45 0.0 0.0 8.590290394766794E-5 0.9419826103888109 2.8634301315889317E-5 46-47 0.0 0.0 8.590290394766794E-5 1.1779578875330547 2.8634301315889317E-5 48-49 0.0 0.0 8.590290394766794E-5 1.4452304560155658 2.8634301315889317E-5 50-51 0.0 0.0 8.590290394766794E-5 1.733620821718545 2.8634301315889317E-5 52-53 0.0 0.0 8.590290394766794E-5 2.0071929364905516 2.8634301315889317E-5 54-55 0.0 0.0 8.590290394766794E-5 2.2855040281303376 2.8634301315889317E-5 56-57 0.0 0.0 8.590290394766794E-5 2.6127225064176627 2.8634301315889317E-5 58-59 0.0 0.0 8.590290394766794E-5 3.006845029729563 2.8634301315889317E-5 60-61 0.0 0.0 8.590290394766794E-5 3.4903495245990124 2.8634301315889317E-5 62-63 0.0 0.0 8.590290394766794E-5 3.9353695184999062 2.8634301315889317E-5 64-65 0.0 0.0 8.590290394766794E-5 4.331797103067736 2.8634301315889317E-5 66-67 0.0 0.0 8.590290394766794E-5 4.7714481654719005 2.8634301315889317E-5 68-69 0.0 0.0 8.590290394766794E-5 5.277616709832875 2.8634301315889317E-5 70-71 0.0 0.0 8.590290394766794E-5 5.869172740717833 2.8634301315889317E-5 72-73 0.0 0.0 8.590290394766794E-5 6.463678104638327 2.8634301315889317E-5 74-75 0.0 0.0 8.590290394766794E-5 7.040945619166656 2.8634301315889317E-5 76-77 0.0 0.0 8.590290394766794E-5 7.612300150473253 2.8634301315889317E-5 78-79 0.0 0.0 8.590290394766794E-5 8.269042168303834 2.8634301315889317E-5 80-81 0.0 0.0 8.590290394766794E-5 8.996840204849793 2.8634301315889317E-5 82-83 0.0 0.0 8.590290394766794E-5 9.732455405654989 2.8634301315889317E-5 84-85 0.0 0.0 8.590290394766794E-5 10.523850225423537 2.8634301315889317E-5 86-87 0.0 0.0 8.590290394766794E-5 11.344509301136926 2.8634301315889317E-5 88-89 0.0 0.0 8.590290394766794E-5 12.116633236119881 2.8634301315889317E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 6290 0.0 25.191444 4 TACCTGG 2505 0.0 22.347633 2 CATATAG 900 0.0 22.139307 3 GAGTACT 16710 0.0 21.63171 12-13 AGTACTT 17520 0.0 21.078653 12-13 ATGGGGA 3130 0.0 20.916645 5 ATGGGAG 6695 0.0 20.762243 5 GTACATG 41915 0.0 20.660816 1 TACATGG 41995 0.0 20.470001 2 GTATAGG 1070 0.0 20.433493 1 ATGGGAT 7950 0.0 19.99102 5 ACATGGG 41325 0.0 19.883476 3 GTACTTT 18330 0.0 19.875568 14-15 GTAAGCG 345 0.0 17.90422 94-95 TACTTTT 20980 0.0 17.557386 14-15 ACTTTTT 21365 0.0 17.5191 16-17 CATGGGA 33490 0.0 17.46647 4 CGTATAG 355 1.5734258E-9 17.405382 1 ATGGGGG 3255 0.0 17.34416 5 GACATAT 1350 0.0 16.899584 1 >>END_MODULE