##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938768_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3492315 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01587399762049 31.0 31.0 34.0 30.0 34.0 2 32.194500782432286 33.0 31.0 34.0 30.0 34.0 3 32.28183711950383 34.0 31.0 34.0 30.0 34.0 4 35.89123317913762 37.0 35.0 37.0 35.0 37.0 5 35.80686822351363 37.0 35.0 37.0 35.0 37.0 6 35.85913870885072 37.0 35.0 37.0 35.0 37.0 7 35.777867403140895 37.0 35.0 37.0 33.0 37.0 8 35.78286637946462 37.0 35.0 37.0 33.0 37.0 9 37.387757977158415 39.0 37.0 39.0 34.0 39.0 10-11 37.3131149395172 39.0 37.0 39.0 34.0 39.0 12-13 37.23940280301176 39.0 37.0 39.0 33.0 39.0 14-15 38.348768510286156 40.0 38.0 41.0 33.0 41.0 16-17 38.301037707079686 40.0 38.0 41.0 33.0 41.0 18-19 38.533749962417474 40.0 38.0 41.0 34.0 41.0 20-21 38.551006853620024 40.0 38.0 41.0 34.0 41.0 22-23 38.4859427056265 40.0 38.0 41.0 34.0 41.0 24-25 38.40067004265079 40.0 38.0 41.0 34.0 41.0 26-27 38.164399116345464 40.0 37.5 41.0 33.0 41.0 28-29 37.9882648042917 40.0 37.0 41.0 33.0 41.0 30-31 37.77831166432581 40.0 37.0 41.0 33.0 41.0 32-33 37.595409348813035 40.0 36.0 41.0 32.5 41.0 34-35 37.55706200614779 40.0 36.0 41.0 33.0 41.0 36-37 37.401315889316976 40.0 36.0 41.0 32.0 41.0 38-39 37.23131261068947 40.0 36.0 41.0 31.5 41.0 40-41 37.04653918675721 40.0 36.0 41.0 31.0 41.0 42-43 36.860304268085784 40.0 36.0 41.0 31.0 41.0 44-45 36.57784721595847 39.5 35.0 41.0 30.0 41.0 46-47 36.177114750530805 39.0 35.0 41.0 30.0 41.0 48-49 35.82641614516446 39.0 35.0 41.0 28.5 41.0 50-51 35.59945108044377 39.0 35.0 41.0 27.5 41.0 52-53 35.33725823701471 39.0 35.0 40.0 27.0 41.0 54-55 35.104593944131615 38.5 34.5 40.0 26.0 41.0 56-57 34.887058727520284 38.0 34.0 40.0 26.0 41.0 58-59 34.62731769614138 38.0 34.0 40.0 26.0 41.0 60-61 34.23102283156015 38.0 34.0 40.0 24.5 41.0 62-63 33.84905156035467 37.0 33.0 40.0 23.0 41.0 64-65 33.41838680073246 36.5 33.0 39.5 22.0 41.0 66-67 33.00035463582179 36.0 32.0 39.0 22.0 40.0 68-69 32.69452555110292 36.0 32.0 39.0 21.5 40.0 70-71 32.244911613070414 35.0 31.5 38.0 20.0 40.0 72-73 31.723525226103604 35.0 31.0 37.0 18.0 39.5 74-75 31.225001897022462 35.0 31.0 37.0 15.0 39.0 76-77 29.233242848941174 32.5 28.5 35.0 9.5 37.0 78-79 30.14259252100684 34.0 30.0 36.0 8.0 38.0 80-81 30.207104026984965 34.0 30.0 35.5 7.0 37.0 82-83 29.89800805482896 34.0 30.0 35.0 4.0 37.0 84-85 29.50785395933643 34.0 30.0 35.0 2.0 36.5 86-87 28.928684984029218 34.0 29.0 35.0 2.0 36.0 88-89 28.287064740723558 34.0 28.5 35.0 2.0 35.5 90-91 27.72494333987627 33.0 26.5 35.0 2.0 35.0 92-93 27.461879297829665 33.0 26.5 35.0 2.0 35.0 94-95 27.14981981865897 33.0 26.0 35.0 2.0 35.0 96-97 26.50892115974647 33.0 25.0 35.0 2.0 35.0 98-99 25.69273791167177 33.0 23.5 34.5 2.0 35.0 100-101 22.157335177382336 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.106751417811914 1101 2 0.10187925830394562 1101 3 0.02854574246040187 1101 4 0.10874553470291204 1101 5 0.015947105906015224 1101 6 0.09429329487859661 1101 7 0.16585686254224186 1101 8 0.17606806508407402 1101 9 0.2436858539074791 1101 10-11 0.28476251459885304 1101 12-13 0.18199019666279526 1101 14-15 0.31741690267644884 1101 16-17 0.37352864893550475 1101 18-19 0.27794873168007683 1101 20-21 0.28587802886534774 1101 22-23 0.21620632915489324 1101 24-25 0.19710083617957252 1101 26-27 0.10578991969146756 1101 28-29 0.15954726232382654 1101 30-31 0.28671003500959813 1101 32-33 0.25018897335775847 1101 34-35 0.20290448874758482 1101 36-37 0.3478189140294745 1101 38-39 0.3600719874289737 1101 40-41 0.3706871525507367 1101 42-43 0.45835523718865545 1101 44-45 0.30979056266664884 1101 46-47 0.3473248350952858 1101 48-49 0.3137680263115783 1101 50-51 0.38915953082672416 1101 52-53 0.2681615197654992 1101 54-55 0.27979740424125055 1101 56-57 0.22502879191564773 1101 58-59 0.27650737057390984 1101 60-61 0.3067730928061607 1101 62-63 0.1637463219833677 1101 64-65 0.27210704392142304 1101 66-67 0.19774786217188023 1101 68-69 0.2223215165050263 1101 70-71 0.3132536483708961 1101 72-73 0.13985616554049685 1101 74-75 0.12034515704092286 1101 76-77 0.10997229506487827 1101 78-79 0.08302790546816752 1101 80-81 0.28396052634229463 1101 82-83 0.2964696435314238 1101 84-85 0.41496572553143807 1101 86-87 0.35334120140090164 1101 88-89 0.4798626644874311 1101 90-91 0.44392884682118705 1101 92-93 0.4491793241958497 1101 94-95 0.5293632833473758 1101 96-97 0.418656842453796 1101 98-99 0.17368306768526764 1101 100-101 0.6473211025226071 1102 1 0.2991686466354224 1102 2 0.2717887574457407 1102 3 0.23378499497430738 1102 4 0.1800876071682893 1102 5 0.07778818940931842 1102 6 0.12934702131880726 1102 7 0.18369471986987662 1102 8 0.24137965085073176 1102 9 0.3057630364258941 1102 10-11 0.2483182883732482 1102 12-13 0.30384689157826017 1102 14-15 0.41423770481457467 1102 16-17 0.45078727237742555 1102 18-19 0.45325007250792027 1102 20-21 0.39208483563140817 1102 22-23 0.31185522676931754 1102 24-25 0.42348619574981683 1102 26-27 0.3490525273748446 1102 28-29 0.40378193329077305 1102 30-31 0.39306808388476355 1102 32-33 0.44091741262550244 1102 34-35 0.49195082537735146 1102 36-37 0.4003818789426816 1102 38-39 0.5319619919065488 1102 40-41 0.6288400903460243 1102 42-43 0.5868921145722865 1102 44-45 0.547655117654692 1102 46-47 0.48123004105839584 1102 48-49 0.4917922090778717 1102 50-51 0.40335773671620245 1102 52-53 0.29133455340230796 1102 54-55 0.3113468308481373 1102 56-57 0.2658723486105856 1102 58-59 0.32007251893919175 1102 60-61 0.2648990328767056 1102 62-63 0.22086921632013912 1102 64-65 0.30475670645792974 1102 66-67 0.40011331295684727 1102 68-69 0.35931557031059214 1102 70-71 0.2692031136635009 1102 72-73 0.3870414288965698 1102 74-75 0.3432408570140062 1102 76-77 0.30394953582965556 1102 78-79 0.2894559209595542 1102 80-81 0.4556946102882016 1102 82-83 0.32966797210849563 1102 84-85 0.4069511036863176 1102 86-87 0.6236947796399761 1102 88-89 0.5067975531865478 1102 90-91 0.6928231460100243 1102 92-93 0.7300916182111585 1102 94-95 0.73343603572226 1102 96-97 0.7361902554655053 1102 98-99 0.8961366567250479 1102 100-101 1.4650439109861324 1103 1 0.09773147646058788 1103 2 0.08616189516757089 1103 3 0.062831548438389 1103 4 -0.07017776871772696 1103 5 -0.09375608045797179 1103 6 -0.05035158379246241 1103 7 0.010623531482110593 1103 8 0.0088003631733784 1103 9 0.009609610631819976 1103 10-11 0.09680198010163821 1103 12-13 0.10508545863623198 1103 14-15 0.13049571734743637 1103 16-17 0.023468195070194042 1103 18-19 -0.1423882775584886 1103 20-21 -0.050775025318486655 1103 22-23 0.06682201988142822 1103 24-25 0.005788180983230973 1103 26-27 0.0700709118248426 1103 28-29 0.0038967313968854 1103 30-31 0.20929398645395025 1103 32-33 0.15674563030313493 1103 34-35 0.08600705171868839 1103 36-37 0.16499346804683057 1103 38-39 0.17344946104308434 1103 40-41 0.18956015129512593 1103 42-43 0.15823108011290543 1103 44-45 0.2694256331292948 1103 46-47 0.2693452859118324 1103 48-49 0.2582332683476807 1103 50-51 0.1500781327257812 1103 52-53 0.1141493407386136 1103 54-55 0.1285810660526252 1103 56-57 0.014198163026968302 1103 58-59 0.10680755390116303 1103 60-61 0.19728809394945301 1103 62-63 0.1972440213667852 1103 64-65 0.3082359506571919 1103 66-67 0.16895974755423282 1103 68-69 0.18693157503335556 1103 70-71 0.1028218866241204 1103 72-73 0.171541171617978 1103 74-75 0.20962324080984374 1103 76-77 0.18524570138144725 1103 78-79 0.041767279909059596 1103 80-81 0.21234544135787914 1103 82-83 0.26383173691198536 1103 84-85 0.2502659289163738 1103 86-87 0.4164485381012497 1103 88-89 0.2857806445982298 1103 90-91 -0.050613617305639735 1103 92-93 0.1462870861191412 1103 94-95 0.21284433485150345 1103 96-97 0.2701668251640683 1103 98-99 0.3637797056159613 1103 100-101 0.9962125142226554 1104 1 0.14556561415388103 1104 2 0.1037369961229544 1104 3 0.0788504591051904 1104 4 0.07901360329054796 1104 5 -0.060205405780536125 1104 6 0.011909816778796767 1104 7 0.056249454854516046 1104 8 0.07228307764201247 1104 9 0.2146012387220395 1104 10-11 0.20434556327902698 1104 12-13 0.037398075596733804 1104 14-15 0.09850714254196191 1104 16-17 0.20000714907393302 1104 18-19 0.26149136392678685 1104 20-21 0.19361284033698212 1104 22-23 0.17127095123176872 1104 24-25 0.17714935502046103 1104 26-27 0.19104106842880242 1104 28-29 0.14236023971955092 1104 30-31 0.03716062685580113 1104 32-33 0.12948373726658957 1104 34-35 0.11319605989361747 1104 36-37 0.25571246735737674 1104 38-39 0.25655297724519244 1104 40-41 0.2839519566728441 1104 42-43 0.3356318483587444 1104 44-45 0.48701550804312177 1104 46-47 0.4264358010305216 1104 48-49 0.5097766545389533 1104 50-51 0.3821464869068194 1104 52-53 0.37166830065194034 1104 54-55 0.3205479723857252 1104 56-57 0.3456440410925623 1104 58-59 0.43450774103796874 1104 60-61 0.46125046960197125 1104 62-63 0.5450170933652174 1104 64-65 0.34948188781862655 1104 66-67 0.3047038625941312 1104 68-69 0.39379654105325557 1104 70-71 0.3798730045202987 1104 72-73 0.38628853692872056 1104 74-75 0.352720847428575 1104 76-77 0.34266813809966123 1104 78-79 0.2657611733395733 1104 80-81 0.4097668931455267 1104 82-83 0.42903315521199303 1104 84-85 0.3942499730412621 1104 86-87 0.5368878171320794 1104 88-89 0.5948676139668869 1104 90-91 0.5331054804979622 1104 92-93 0.5094546228078052 1104 94-95 0.5735475752730537 1104 96-97 0.5523277628179635 1104 98-99 0.9634586182526164 1104 100-101 1.8211430766180072 1105 1 0.03381066251868958 1105 2 0.028851522315321176 1105 3 0.008037679070852732 1105 4 0.02443883673973346 1105 5 -0.06641787594509907 1105 6 -0.0776408720533297 1105 7 -0.07773823410322933 1105 8 0.04460751966705345 1105 9 0.06639555219300064 1105 10-11 0.05291597490332833 1105 12-13 0.03239828496820252 1105 14-15 -0.01969519404361364 1105 16-17 -0.030840805366835866 1105 18-19 0.028475577318182843 1105 20-21 -0.0288790988615375 1105 22-23 -0.061624237411926686 1105 24-25 0.01558576890597152 1105 26-27 0.053218112941173956 1105 28-29 0.05494053367708318 1105 30-31 0.007228463211070846 1105 32-33 0.07494163266460419 1105 34-35 0.026849591990263377 1105 36-37 0.03739120777863292 1105 38-39 0.06128058496681632 1105 40-41 1.1962134350085307E-4 1105 42-43 0.07469009550082006 1105 44-45 -0.02949008217505167 1105 46-47 -0.05225269610109251 1105 48-49 -0.056838227584918855 1105 50-51 -0.049302363643832336 1105 52-53 0.05130399429961585 1105 54-55 0.017295596019579307 1105 56-57 -0.011251398796886747 1105 58-59 0.009877700419899327 1105 60-61 0.08889420311396634 1105 62-63 0.01651242438150291 1105 64-65 0.03958950475258405 1105 66-67 0.04582274371301054 1105 68-69 0.012622804610053606 1105 70-71 -0.006015777356502383 1105 72-73 0.13221081824022818 1105 74-75 0.017953877871189405 1105 76-77 0.11021974816623725 1105 78-79 -0.12955016203164504 1105 80-81 0.03687379821650438 1105 82-83 0.16574560443941522 1105 84-85 0.056462191960068964 1105 86-87 0.10240643025548479 1105 88-89 0.14275673083804108 1105 90-91 0.03789129348736964 1105 92-93 0.08497700461761326 1105 94-95 0.2367827831789171 1105 96-97 0.2706888029754104 1105 98-99 0.3731227864197777 1105 100-101 1.1568790173240053 1106 1 0.14291688235950772 1106 2 0.042959780390489755 1106 3 0.07019430376067248 1106 4 0.043705651139632096 1106 5 -0.018884567681830333 1106 6 -4.131247223355672E-4 1106 7 0.03259734889159205 1106 8 0.0015125571400034232 1106 9 0.046862386849745974 1106 10-11 0.0853526549126471 1106 12-13 0.11139858010984938 1106 14-15 0.16148889092647067 1106 16-17 0.14121016628996585 1106 18-19 -0.024952406569063612 1106 20-21 0.03772041163739459 1106 22-23 0.048042256953443996 1106 24-25 0.08772383614009982 1106 26-27 0.16620498777498227 1106 28-29 0.21247785196897695 1106 30-31 0.10166592898519156 1106 32-33 0.139412365730621 1106 34-35 0.012147507144490532 1106 36-37 0.026684793105943072 1106 38-39 0.19826122831064907 1106 40-41 0.3766634491402385 1106 42-43 0.21184064092229704 1106 44-45 0.2617074989922301 1106 46-47 0.2541622866414599 1106 48-49 0.2930221153434047 1106 50-51 0.2794980206456614 1106 52-53 0.27503731026993705 1106 54-55 0.2954277332541011 1106 56-57 0.1954447584115826 1106 58-59 0.33511630524295555 1106 60-61 0.403994603688659 1106 62-63 0.38489312728518854 1106 64-65 0.39546382017972803 1106 66-67 0.4290364922644301 1106 68-69 0.311876394478908 1106 70-71 0.46482038152626615 1106 72-73 0.35025438935673847 1106 74-75 0.500679712539597 1106 76-77 0.3484632109411123 1106 78-79 0.25566051728285544 1106 80-81 0.2501820733003335 1106 82-83 0.38414662324654003 1106 84-85 0.2968380494617229 1106 86-87 0.4614894698867005 1106 88-89 0.4724426508096222 1106 90-91 0.37770774018565945 1106 92-93 0.6305819630563825 1106 94-95 0.7676360850816586 1106 96-97 0.838144836983286 1106 98-99 1.0077909823897002 1106 100-101 1.625660479475524 1107 1 -0.021710131683015277 1107 2 -0.011888098207180064 1107 3 -0.09111281503074764 1107 4 -0.08811926384232294 1107 5 -0.14722155509884516 1107 6 -0.118769863253398 1107 7 -0.04441563545086069 1107 8 -0.08232476056294757 1107 9 -0.05430755033063406 1107 10-11 -0.09964548164726494 1107 12-13 -0.003518561722778202 1107 14-15 -0.07181181907196077 1107 16-17 -0.06768717099448907 1107 18-19 -0.08204890875017412 1107 20-21 -0.17177730908193922 1107 22-23 -0.0902292751605458 1107 24-25 -0.11293969262254677 1107 26-27 -0.1591601691825062 1107 28-29 -0.12988355964655796 1107 30-31 -0.1470105257326182 1107 32-33 -0.21404597043577667 1107 34-35 -0.2206410382600339 1107 36-37 -0.26101088123647287 1107 38-39 -0.2534007381042045 1107 40-41 -0.4676026038745249 1107 42-43 -0.5058608316231457 1107 44-45 -0.31630692491960843 1107 46-47 -0.2751172502347714 1107 48-49 -0.2733701986079069 1107 50-51 -0.38499946067078383 1107 52-53 -0.45547060360926395 1107 54-55 -0.3861225513351556 1107 56-57 -0.35971089174438475 1107 58-59 -0.3303224647274874 1107 60-61 -0.3747197472315591 1107 62-63 -0.3803987138491536 1107 64-65 -0.2811932643673174 1107 66-67 -0.35035122620196546 1107 68-69 -0.33650833392788826 1107 70-71 -0.3809545851562035 1107 72-73 -0.3647156387517114 1107 74-75 -0.2744583043548907 1107 76-77 -0.22148641238582556 1107 78-79 -0.15097978354527086 1107 80-81 -0.1515612750891151 1107 82-83 -0.2125093310786923 1107 84-85 -0.29013750997931353 1107 86-87 -0.2971263701195177 1107 88-89 -0.43929850768124723 1107 90-91 -0.17099071108023978 1107 92-93 -0.0651491906449273 1107 94-95 0.0024724633346302483 1107 96-97 0.11905362688196064 1107 98-99 0.4685891711529564 1107 100-101 1.1250405460550432 1108 1 0.20480517600213943 1108 2 0.11314986292712348 1108 3 0.06841238953668238 1108 4 0.0745174531422208 1108 5 0.04709263108271955 1108 6 0.049303668520472854 1108 7 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-0.9327024595338926 2316 54-55 -0.8653838243050203 2316 56-57 -0.9607943468156321 2316 58-59 -0.7942784060057946 2316 60-61 -0.7728372438604083 2316 62-63 -0.7906775540504327 2316 64-65 -0.8554710373961925 2316 66-67 -0.8028119193257126 2316 68-69 -0.8276690377292724 2316 70-71 -0.7369975968184974 2316 72-73 -0.6915257279085232 2316 74-75 -0.7442549293334118 2316 76-77 -0.8968733733018226 2316 78-79 -0.8456628809599529 2316 80-81 -0.9263441951248303 2316 82-83 -0.8999816941823298 2316 84-85 -1.1019665896584634 2316 86-87 -1.2535352018585506 2316 88-89 -1.5073268297508058 2316 90-91 -1.9664683609465357 2316 92-93 -1.6977514260871764 2316 94-95 -1.6913116862564408 2316 96-97 -1.884661955107081 2316 98-99 -2.0153372838677086 2316 100-101 -1.971384086224493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 18.0 4 45.0 5 81.0 6 300.0 7 701.0 8 1477.0 9 2723.0 10 4595.0 11 7088.0 12 10253.0 13 14348.0 14 16930.0 15 22858.0 16 29615.0 17 41220.0 18 37645.0 19 23503.0 20 21300.0 21 22085.0 22 23856.0 23 26250.0 24 30209.0 25 36082.0 26 43100.0 27 52319.0 28 63526.0 29 76872.0 30 93324.0 31 111704.0 32 138672.0 33 173448.0 34 228115.0 35 318215.0 36 464737.0 37 701704.0 38 595330.0 39 58064.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.827768306613166 19.86527985856394 14.416174453111996 20.890777381710894 2 14.483372777083394 22.79281794454395 39.59674313456833 23.127066143804324 3 21.16441386301064 27.94301201352112 29.92058276530038 20.971991358167863 4 12.221291607429457 16.985208951655277 38.76783738007597 32.0256620608393 5 11.991669774318593 40.544030988302055 33.73326092647553 13.73103831090382 6 28.44173910453847 37.35245639696373 18.374096698115906 15.831707800381881 7 26.509550255346383 35.322815954460005 21.00901550976931 17.158618280424303 8 25.237356882182738 33.420610683744165 20.576608925598062 20.76542350847504 9 26.986511812365148 18.414117855920786 21.474007928838034 33.12536240287603 10-11 23.94458976352362 27.081348618323375 27.18712372738427 21.786937890768733 12-13 27.873530881378112 24.789960241272624 27.13303353219856 20.20347534515071 14-15 24.40338285635746 25.427875205988006 28.15514923481988 22.013592702834654 16-17 20.360524922087382 29.291651269316727 29.273840216241076 21.07398359235482 18-19 20.134652801937968 29.257054418057937 31.200364228312736 19.407928551691356 20-21 21.85042013678606 27.261887888120057 29.742606265471473 21.145085709622414 22-23 20.8338308543187 27.35043946493945 29.133568993633162 22.682160687108695 24-25 21.556322798497554 27.482842623169095 30.0329080885748 20.927926489758548 26-27 22.323125811164775 26.043448723648105 31.179939165773625 20.45348629941349 28-29 20.582650763175717 27.044639444036406 31.73604042017974 20.63666937260814 30-31 20.65876073607335 28.14194882191326 30.723731965759104 20.475558476254292 32-33 20.421926429889627 27.96261219277184 30.285841912885864 21.329619464452662 34-35 21.3202374002975 26.782546144106522 30.28793899560906 21.609277459986917 36-37 22.151701704654478 26.46298372098218 30.935489358582295 20.449825215781043 38-39 22.41972265234919 26.149035629404704 31.19525307837106 20.235988639875046 40-41 20.93721958390565 27.04424272423372 31.56398995939457 20.45454773246606 42-43 20.361331341002927 28.092173726782317 30.291478549325358 21.2550163828894 44-45 21.055833452766215 27.920534872230203 29.18565482460404 21.83797685039954 46-47 21.16202129603063 27.893069712916496 28.536901532345215 22.408007458707658 48-49 21.73659750419499 27.491767508719285 28.900969572712228 21.870665414373498 50-51 21.80878898506993 28.10478552260972 29.153330835467422 20.933094656852926 52-53 20.926090573158493 28.646027062278172 29.141228669235165 21.28665369532817 54-55 19.877702899079836 27.830736918061515 29.779773588579495 22.511786594279155 56-57 21.413418576825592 28.570639072775144 27.98294118396613 22.033001166433127 58-59 21.449493979829633 27.568682142707324 28.550713619657408 22.431110257805635 60-61 21.134564386916242 27.338077376720204 29.44852858008859 22.078829656274955 62-63 22.179380240650048 27.087948856495636 29.057429635731957 21.675241267122356 64-65 21.321329490370232 27.745402310617116 29.434546951617868 21.498721247394784 66-67 20.698511514429548 27.757807533489494 29.078215650002576 22.465465302078382 68-69 20.425463679816282 28.64852250267194 28.7452207458822 22.180793071629573 70-71 20.845832216594623 28.502235768167335 27.806736626051737 22.845195389186305 72-73 20.9813404575475 27.581675765215905 27.852184009747116 23.58479976748947 74-75 20.915264749516147 28.573238675589536 27.879212015230078 22.632284559664246 76-77 21.381047488862613 27.246752472802253 28.709561521154086 22.662638517181044 78-79 20.84285321037781 27.280010552152866 29.224216043860253 22.652920193609067 80-81 20.538332815047582 28.730769737380886 28.306376747353163 22.424520700218366 82-83 20.361776839329828 28.46495323556943 27.908570620260452 23.26469930484029 84-85 20.548991141978888 27.673735043946497 27.671816545758332 24.10545726831629 86-87 20.97858028994547 27.725992473892525 28.02484681780032 23.270580418361682 88-89 21.449243836111638 26.747829325368507 28.395072193942095 23.407854644577757 90-91 20.63670951919438 27.10445973777375 28.81839795085166 23.440432792180214 92-93 20.235326053420042 28.402729029707757 28.307061513983534 23.054883402888667 94-95 20.060962602063682 28.38434787339594 27.715878195060284 23.8388113294801 96-97 19.927595264227463 27.693880817670124 27.547817163009157 24.830706755093257 98-99 20.639905048656836 27.456228891150996 27.138345195092654 24.76552086509951 100-101 20.98846139308577 27.208430834218145 27.895847865403532 23.90725990729255 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3140.0 1 2083.0 2 1342.5 3 1716.0 4 1475.0 5 1438.5 6 2686.0 7 5362.5 8 7762.0 9 8113.0 10 6755.5 11 5610.0 12 5677.0 13 5937.5 14 6088.0 15 6206.5 16 6400.0 17 6792.5 18 7147.0 19 7632.5 20 8473.0 21 9612.0 22 10954.5 23 12546.0 24 14146.5 25 16497.0 26 20756.0 27 25713.5 28 29841.0 29 34403.5 30 40510.0 31 47345.0 32 56461.5 33 67217.0 34 77823.5 35 85809.0 36 94891.5 37 104504.5 38 105443.0 39 103205.5 40 101920.5 41 101589.0 42 101339.0 43 103098.0 44 109157.5 45 112892.5 46 120100.0 47 149400.0 48 255868.0 49 258605.0 50 169353.0 51 134522.5 52 95567.0 53 80868.0 54 76007.5 55 70183.0 56 62516.5 57 54710.0 58 48940.5 59 44005.5 60 38299.5 61 31933.5 62 25825.5 63 19418.0 64 13970.0 65 10753.0 66 8190.5 67 6488.5 68 5094.0 69 3558.0 70 2689.0 71 2259.5 72 1912.5 73 1782.5 74 1469.5 75 1124.5 76 874.5 77 611.5 78 422.5 79 308.5 80 224.5 81 159.5 82 117.5 83 79.0 84 53.5 85 35.0 86 23.5 87 16.5 88 9.5 89 5.0 90 5.5 91 4.0 92 2.0 93 2.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23064929709948845 2 0.0 3 0.0 4 0.0 5 0.026715803127724733 6 0.003636556267117943 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002906381583562766 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.5792876644861647E-4 26-27 0.0010737862993458493 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006829280863839602 36-37 0.016636529064531693 38-39 0.012942704194781973 40-41 0.008432801737529404 42-43 0.007359015438183554 44-45 0.003335896103301105 46-47 6.013203276336756E-4 48-49 0.0015748865723739125 50-51 1.431715065794466E-5 52-53 0.0 54-55 0.0 56-57 0.0012742264085570745 58-59 3.865630677645058E-4 60-61 0.0021762069000075884 62-63 6.872232315813436E-4 64-65 0.0012742264085570745 66-67 0.00501100273028063 68-69 2.1475725986916987E-4 70-71 8.590290394766794E-5 72-73 0.0 74-75 4.008802184224504E-4 76-77 0.05852851188967777 78-79 0.1395922189149604 80-81 9.449319434243475E-4 82-83 6.872232315813436E-4 84-85 0.0 86-87 0.10477290851483902 88-89 0.35359066979925924 90-91 0.38946944934806854 92-93 3.2929446513272714E-4 94-95 2.8634301315889313E-4 96-97 5.726860263177864E-5 98-99 0.0 100-101 0.0011167377513196834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3492315.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.546099705153175 #Duplication Level Percentage of deduplicated Percentage of total 1 65.03510458123819 21.16638998036042 2 14.948086363173104 9.73003818354145 3 6.690595919166084 6.53258405616211 4 3.770117450793449 4.908104738146461 5 2.331040313703074 3.7933135233255886 6 1.561507967496407 3.049259640031749 7 1.0915858494754165 2.4868803325632864 8 0.7812491564607709 2.034129035259125 9 0.6012765921667202 1.761228712712952 >10 2.8553766760143695 16.093650849550333 >50 0.18728929043069137 4.263378905320186 >100 0.12809435092371713 7.948701055025928 >500 0.00951392833717935 2.1362953318126108 >1k 0.006871170465574976 4.747459940713083 >5k 0.0011451950775958296 2.5790249929457305 >10k+ 0.0011451950775958296 6.769560722529004 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 38042 1.0893060906590615 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 32963 0.9438724742756595 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22449 0.6428114302403993 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21350 0.6113423330942369 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 19130 0.5477741841729626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15658 0.44835589000419496 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14718 0.4214396467672589 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 14570 0.4172017701725073 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 12360 0.353919964264392 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12003 0.34369751869461945 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 11118 0.31835616203005745 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11037 0.3160367836234704 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 10718 0.3069024415037017 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9762 0.2795280494457115 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 9486 0.2716249822825261 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8714 0.24951930166665948 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8418 0.24104354847715626 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 7300 0.209030399605992 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 6456 0.18486304929538142 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 6130 0.1755282670664015 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6041 0.17297981424928738 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 6036 0.17283664274270794 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5924 0.1696296009953283 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5400 0.15462522710580232 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5184 0.14844021802157023 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5103 0.14612083961498318 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4999 0.1431428722781307 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4861 0.13919133869653796 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4673 0.13380809004915076 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4458 0.12765171526623456 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4358 0.12478828513464565 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4163 0.11920459637804721 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4012 0.11488081687934795 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3908 0.11190284954249545 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3762 0.1077222415503756 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3664 0.10491608002141846 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3651 0.1045438341043119 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3513 0.10059230052271917 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.8634301315889317E-5 0.0 10-11 0.0 0.0 2.8634301315889317E-5 4.295145197383397E-5 0.0 12-13 0.0 0.0 2.8634301315889317E-5 7.158575328972328E-5 0.0 14-15 0.0 0.0 2.8634301315889317E-5 1.1453720526355727E-4 0.0 16-17 0.0 0.0 2.8634301315889317E-5 3.7224591710656116E-4 0.0 18-19 0.0 0.0 2.8634301315889317E-5 7.015403822392882E-4 0.0 20-21 0.0 0.0 2.8634301315889317E-5 0.0011883235046094066 0.0 22-23 0.0 0.0 2.8634301315889317E-5 0.0025055013651403154 0.0 24-25 0.0 0.0 2.8634301315889317E-5 0.004266510896067508 0.0 26-27 0.0 0.0 2.8634301315889317E-5 0.006857915165155491 0.0 28-29 0.0 0.0 2.8634301315889317E-5 0.014374419260576438 0.0 30-31 0.0 0.0 2.8634301315889317E-5 0.03517723916657003 0.0 32-33 0.0 0.0 2.8634301315889317E-5 0.07645358451342447 0.0 34-35 0.0 0.0 2.8634301315889317E-5 0.14710872301038136 0.0 36-37 0.0 0.0 2.8634301315889317E-5 0.2590974754568245 0.0 38-39 0.0 0.0 2.8634301315889317E-5 0.41282072207117626 0.0 40-41 0.0 0.0 2.8634301315889317E-5 0.5956507359731296 0.0 42-43 0.0 0.0 2.8634301315889317E-5 0.769117333344787 0.0 44-45 0.0 0.0 2.8634301315889317E-5 0.959277728383608 0.0 46-47 0.0 0.0 2.8634301315889317E-5 1.1984170958232576 0.0 48-49 0.0 0.0 4.295145197383397E-5 1.4693405377235444 0.0 50-51 0.0 0.0 5.7268602631778634E-5 1.7621549029798285 0.0 52-53 0.0 0.0 5.7268602631778634E-5 2.038848156595267 0.0 54-55 0.0 0.0 5.7268602631778634E-5 2.3203949242837485 0.0 56-57 0.0 0.0 8.590290394766794E-5 2.6516222047552986 0.0 58-59 0.0 0.0 8.590290394766794E-5 3.050326216277741 0.0 60-61 0.0 0.0 8.590290394766794E-5 3.539600522862342 0.0 62-63 0.0 0.0 8.590290394766794E-5 3.991220723216548 5.7268602631778634E-5 64-65 0.0 0.0 8.590290394766794E-5 4.394019439827163 5.7268602631778634E-5 66-67 0.0 0.0 8.590290394766794E-5 4.839483265398454 5.7268602631778634E-5 68-69 0.0 0.0 8.590290394766794E-5 5.351707964487739 5.7268602631778634E-5 70-71 0.0 0.0 8.590290394766794E-5 5.9517540657128585 5.7268602631778634E-5 72-73 0.0 0.0 8.590290394766794E-5 6.555766017670227 5.7268602631778634E-5 74-75 0.0 0.0 8.590290394766794E-5 7.142210825770299 5.7268602631778634E-5 76-77 0.0 0.0 8.590290394766794E-5 7.721797718705215 5.7268602631778634E-5 78-79 0.0 0.0 8.590290394766794E-5 8.386356900795032 5.7268602631778634E-5 80-81 0.0 0.0 8.590290394766794E-5 9.121027169656802 5.7268602631778634E-5 82-83 0.0 0.0 8.590290394766794E-5 9.866363715758744 5.7268602631778634E-5 84-85 0.0 0.0 8.590290394766794E-5 10.666062482908902 5.7268602631778634E-5 86-87 0.0 0.0 8.590290394766794E-5 11.497273298657195 5.7268602631778634E-5 88-89 0.0 0.0 8.590290394766794E-5 12.281294785836902 5.7268602631778634E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCT 120 1.7218286E-4 23.729523 3 TACCTGG 2380 0.0 22.333668 2 CATTCCG 805 0.0 21.806917 9 GAGTACT 17285 0.0 21.534294 12-13 GTAAGCG 290 0.0 21.268805 94-95 AGTACTT 18200 0.0 21.025196 12-13 TTAGACG 140 4.8827802E-4 20.33959 4 GTACTTT 18850 0.0 19.683508 14-15 ATGGGAT 8130 0.0 19.438946 5 CATGGGG 5880 0.0 19.209614 4 CATATAG 1005 0.0 18.459507 1 GTACATG 42745 0.0 18.328623 1 TAGACGG 130 0.007603864 18.253479 5 ATGGGAG 6700 0.0 18.133606 5 TACATGG 43040 0.0 18.10589 2 ACATGGG 41645 0.0 17.789309 3 TACTTTT 21375 0.0 17.70238 14-15 GTACCGG 215 2.3670911E-5 17.699991 1 ACTTTTT 21290 0.0 17.694675 16-17 GTGTAGC 1315 0.0 17.363491 1 >>END_MODULE