FastQCFastQC Report
Thu 26 May 2016
SRR938767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4293800
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96640.2250687037123294No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87340.2034095672830593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.18019004145512135No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51600.12017327309143416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49510.11530578974335089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG147300.023.364251
TACATGG141850.022.914492
ACATGGG145400.021.8010353
GTACCGT3800.021.181016
GTATCAA239250.020.6657121
GAGTACT114000.020.22696512-13
GTATAGC15650.018.7799911
TAGCCCT30750.018.3365354
GTACTTT125350.018.1692714-15
TCAACGC267750.018.156554
CAACGCA267750.018.1211555
ATCAACG268550.018.102463
AGAGTAC186350.018.09035710-11
TATCAAC269400.018.0629332
AGTACTT122550.018.0237412-13
CATGGGG77400.017.8754634
AACGCAG273050.017.7730926
GGTATCA188000.017.4992581
GTGTAGC29100.017.4304581
GAGTACA124000.017.394331
Produced by FastQC (version 0.11.5)