##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938767_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4293800 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.159164842330803 31.0 31.0 34.0 28.0 34.0 2 31.22927267222507 33.0 31.0 34.0 27.0 34.0 3 31.243913549769434 33.0 31.0 34.0 27.0 34.0 4 34.78385253155713 37.0 35.0 37.0 32.0 37.0 5 34.813217895570354 37.0 35.0 37.0 32.0 37.0 6 34.880660953002 37.0 35.0 37.0 32.0 37.0 7 34.768776608132654 37.0 35.0 37.0 32.0 37.0 8 34.72829591504029 37.0 35.0 37.0 31.0 37.0 9 36.23257534118962 39.0 37.0 39.0 32.0 39.0 10-11 36.11000104802273 39.0 35.0 39.0 30.0 39.0 12-13 35.97153873026224 39.0 35.0 39.0 30.0 39.0 14-15 37.05246681261353 40.0 36.0 41.0 31.0 41.0 16-17 36.96787530858447 40.0 36.0 41.0 31.0 41.0 18-19 36.88077274209326 40.0 36.0 41.0 30.0 41.0 20-21 36.74782139363734 39.5 36.0 41.0 30.0 41.0 22-23 36.652012436536396 39.0 36.0 41.0 30.0 41.0 24-25 36.66250477432577 39.0 36.0 41.0 30.0 41.0 26-27 36.55757720434114 39.0 36.0 41.0 30.0 41.0 28-29 36.43026386883413 39.0 36.0 41.0 29.0 41.0 30-31 36.22571323769156 39.0 35.5 41.0 28.5 41.0 32-33 36.018818179700965 39.0 35.0 41.0 27.5 41.0 34-35 35.67848420979087 39.0 35.0 41.0 26.5 41.0 36-37 35.42791606502399 39.0 35.0 40.0 25.5 41.0 38-39 35.24610508174577 38.0 34.5 40.0 25.0 41.0 40-41 35.0660762028972 38.0 34.0 40.0 25.0 41.0 42-43 34.806643183194375 38.0 34.0 40.0 24.0 41.0 44-45 34.79452710885462 38.0 34.0 40.0 24.0 41.0 46-47 34.64952012203642 38.0 34.0 40.0 23.5 41.0 48-49 34.43605407797289 38.0 34.0 40.0 23.0 41.0 50-51 33.31566002142624 37.0 32.0 39.5 20.5 40.5 52-53 33.428493059760584 37.5 33.0 39.5 20.5 40.5 54-55 33.951134426382225 38.0 33.0 40.0 21.0 41.0 56-57 33.84813463598677 38.0 33.0 40.0 20.0 41.0 58-59 33.6153658763799 38.0 33.0 40.0 19.0 41.0 60-61 33.31601518468489 37.0 33.0 40.0 18.0 41.0 62-63 32.945137174530714 37.0 32.0 40.0 15.5 41.0 64-65 32.545490474637845 36.0 31.5 39.5 13.0 41.0 66-67 32.13327402300992 36.0 31.0 39.0 10.0 41.0 68-69 31.721455470678652 35.0 31.0 39.0 8.0 40.0 70-71 31.308774162746285 35.0 30.5 38.0 7.0 40.0 72-73 30.890484535842376 35.0 30.0 37.5 4.0 40.0 74-75 30.43761714565187 35.0 30.0 37.0 2.0 39.0 76-77 29.998071405282033 34.5 29.0 36.0 2.0 39.0 78-79 29.57478748427966 34.0 29.0 36.0 2.0 38.5 80-81 29.139640877544366 34.0 29.0 35.5 2.0 37.0 82-83 28.669830336764637 34.0 28.5 35.0 2.0 37.0 84-85 28.26485548931017 34.0 27.5 35.0 2.0 36.5 86-87 27.87867040849597 34.0 27.0 35.0 2.0 36.0 88-89 27.49370976757185 34.0 26.0 35.0 2.0 36.0 90-91 27.071876193581446 33.0 25.5 35.0 2.0 35.0 92-93 26.610098397689693 33.0 25.0 35.0 2.0 35.0 94-95 26.09348665517723 33.0 24.0 35.0 2.0 35.0 96-97 25.48304066328194 32.5 21.5 35.0 2.0 35.0 98-99 24.73985467418138 32.0 10.0 35.0 2.0 35.0 100-101 22.542671526386883 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4268051238783741 1101 2 0.20523599593885677 1101 3 0.4495658124165338 1101 4 0.36310016176015836 1101 5 0.20441660213216295 1101 6 0.15277280949556626 1101 7 0.3112863853783949 1101 8 0.3818968574653212 1101 9 0.6196402868971589 1101 10-11 0.5802539170865586 1101 12-13 0.5361047488496666 1101 14-15 0.76295359851526 1101 16-17 0.731158285487794 1101 18-19 0.7284534667016587 1101 20-21 0.8369291652166808 1101 22-23 0.7063588006366501 1101 24-25 0.719571118264767 1101 26-27 0.7457261911031736 1101 28-29 0.6148712213128107 1101 30-31 0.7031568465445943 1101 32-33 0.6704345510428595 1101 34-35 0.6574461580850155 1101 36-37 0.7192574005430927 1101 38-39 0.8413954487722108 1101 40-41 0.6692872047305585 1101 42-43 0.6222720712650229 1101 44-45 0.7611922574938035 1101 46-47 0.7834132166529812 1101 48-49 0.7953174299913783 1101 50-51 0.7271556693760601 1101 52-53 0.7849632527831147 1101 54-55 0.7883224905865163 1101 56-57 0.8084352951460758 1101 58-59 0.7368998680152714 1101 60-61 0.6338986210330262 1101 62-63 0.5931151592806714 1101 64-65 0.6070545447728932 1101 66-67 0.504366051914289 1101 68-69 0.4351103317288221 1101 70-71 0.24950514680000424 1101 72-73 0.20377611064840195 1101 74-75 0.20533470270200382 1101 76-77 0.15942539207581063 1101 78-79 0.10776319469502837 1101 80-81 0.02714490929168889 1101 82-83 -0.0850526080557934 1101 84-85 -0.08734768734316489 1101 86-87 0.073094377582688 1101 88-89 -0.06592985266022922 1101 90-91 -0.19448280923747419 1101 92-93 -0.22359903225806121 1101 94-95 -0.2467451796409712 1101 96-97 -0.370088327619424 1101 98-99 -0.49689033730472687 1101 100-101 -0.2582422949071912 1102 1 0.27051265711323325 1102 2 0.33747014708391276 1102 3 0.37598269224264413 1102 4 0.3151158695095546 1102 5 0.2960073119831321 1102 6 0.3146048998991873 1102 7 0.2916659893221407 1102 8 0.2863814919719374 1102 9 0.40870670858058133 1102 10-11 0.3231615920274109 1102 12-13 0.3299372355497212 1102 14-15 0.21593068537622884 1102 16-17 0.4376182775618602 1102 18-19 0.5372576122024952 1102 20-21 0.4570988521425079 1102 22-23 0.46778768944547977 1102 24-25 0.39031939797799 1102 26-27 0.3778098096498468 1102 28-29 0.3790987434994051 1102 30-31 0.41710035628987185 1102 32-33 0.43361326872368977 1102 34-35 0.4691988090679544 1102 36-37 0.5078148815388843 1102 38-39 0.4839608076606581 1102 40-41 0.5484319249813083 1102 42-43 0.5364971316101617 1102 44-45 0.6250890522963175 1102 46-47 0.5860721855617257 1102 48-49 0.5702952734182603 1102 50-51 0.5560787038058166 1102 52-53 0.615155456551534 1102 54-55 0.5203004901301753 1102 56-57 0.5070221981495209 1102 58-59 0.3939098714978755 1102 60-61 0.38129633452271605 1102 62-63 0.39302166935020466 1102 64-65 0.27496450142046314 1102 66-67 0.3383828164541711 1102 68-69 0.22640982374885255 1102 70-71 0.23696920991921644 1102 72-73 0.27136975069115365 1102 74-75 0.07012526604338376 1102 76-77 0.06453845664810842 1102 78-79 -0.01062291811735605 1102 80-81 -0.009550879158069847 1102 82-83 -0.037921554027377624 1102 84-85 0.024157938865240425 1102 86-87 0.04085121970058836 1102 88-89 1.0508147050103389E-4 1102 90-91 0.0237135016030372 1102 92-93 -0.0867487482456184 1102 94-95 -0.18855193109105883 1102 96-97 -0.1537143198616171 1102 98-99 -0.1850753597014787 1102 100-101 0.06338318094874751 1103 1 0.12668877291540426 1103 2 0.22382073109638867 1103 3 0.16935290025111627 1103 4 0.26883913291830197 1103 5 0.2017076856334299 1103 6 0.2684699738937155 1103 7 0.302189688165889 1103 8 0.214886008138933 1103 9 0.31472188996279016 1103 10-11 0.3134897864432489 1103 12-13 0.304644493780323 1103 14-15 0.48186098635596153 1103 16-17 0.5414645219350476 1103 18-19 0.5495889546956576 1103 20-21 0.5081992081400273 1103 22-23 0.5022177991868659 1103 24-25 0.5158579545698316 1103 26-27 0.5492501285765599 1103 28-29 0.4598628142281669 1103 30-31 0.4261647594791427 1103 32-33 0.3841429989305212 1103 34-35 0.42476840511373126 1103 36-37 0.45742710902424477 1103 38-39 0.29073829449463773 1103 40-41 0.4516435522724862 1103 42-43 0.3977135545705295 1103 44-45 0.4788329106034155 1103 46-47 0.4441575955473027 1103 48-49 0.3962060092445583 1103 50-51 0.44051043171779014 1103 52-53 0.34733013071081587 1103 54-55 0.5000304027489619 1103 56-57 0.48787865170803 1103 58-59 0.446185019339822 1103 60-61 0.31458519268270635 1103 62-63 0.23083140191074136 1103 64-65 0.17503599513901946 1103 66-67 0.14162974928537864 1103 68-69 0.07722332223365669 1103 70-71 0.028589145780820502 1103 72-73 0.21706806485162033 1103 74-75 0.1406164543690167 1103 76-77 0.023684651005108748 1103 78-79 0.2146402552622675 1103 80-81 0.03050928736639591 1103 82-83 0.1536477772268512 1103 84-85 0.3178701692927284 1103 86-87 0.15089358634862293 1103 88-89 0.04264222584074062 1103 90-91 -0.2015762980380238 1103 92-93 -0.15108838754068543 1103 94-95 -0.15136607684409498 1103 96-97 -0.23100461966449615 1103 98-99 -0.08466802203340862 1103 100-101 -0.1423502916453252 1104 1 0.2293924844099493 1104 2 0.27282207683252224 1104 3 0.1862001509535709 1104 4 0.3316859073615248 1104 5 0.18878647760160305 1104 6 0.23864306779346123 1104 7 0.258632917110873 1104 8 0.18093619012145723 1104 9 0.27801121513008553 1104 10-11 0.2977748232520696 1104 12-13 0.30510660411795243 1104 14-15 0.41122876235556305 1104 16-17 0.44816727743900486 1104 18-19 0.3888944321686836 1104 20-21 0.4645531167786956 1104 22-23 0.5644678607446778 1104 24-25 0.4427015566520609 1104 26-27 0.5304725234081928 1104 28-29 0.6371005454171623 1104 30-31 0.569665397915351 1104 32-33 0.5493844661214382 1104 34-35 0.5440805143373879 1104 36-37 0.6411127420540694 1104 38-39 0.6484388072979428 1104 40-41 0.4148136390263133 1104 42-43 0.5998227521894677 1104 44-45 0.6373649095235834 1104 46-47 0.5904948698341386 1104 48-49 0.6895037166900337 1104 50-51 0.7498389785397706 1104 52-53 0.7895037824646352 1104 54-55 0.7329182437003681 1104 56-57 0.7335345512317986 1104 58-59 0.7453474859459774 1104 60-61 0.7186209254347347 1104 62-63 0.6922395609894281 1104 64-65 0.6517585504115644 1104 66-67 0.6511364159018882 1104 68-69 0.5707738579384909 1104 70-71 0.5859671564350215 1104 72-73 0.5569961803783379 1104 74-75 0.5699218189981181 1104 76-77 0.5376847337573203 1104 78-79 0.5377414319929237 1104 80-81 0.5775864995196258 1104 82-83 0.46826317764483605 1104 84-85 0.5993247332652523 1104 86-87 0.5367637330833368 1104 88-89 0.4810082342415356 1104 90-91 0.39592901213665144 1104 92-93 0.36799948055113063 1104 94-95 0.4130329959839294 1104 96-97 0.4841129651514571 1104 98-99 0.4238449814928167 1104 100-101 0.6543146495702246 1105 1 0.20456993634951104 1105 2 0.2899394409892615 1105 3 0.2884025071329148 1105 4 0.33978552432066067 1105 5 0.26384886271367236 1105 6 0.22762195560673604 1105 7 0.2704825677027216 1105 8 0.3105586850565132 1105 9 0.31106132759216365 1105 10-11 0.3193001914802309 1105 12-13 0.33669694216825974 1105 14-15 0.4884992283647662 1105 16-17 0.3507174102238366 1105 18-19 0.2809871657271472 1105 20-21 0.39245015314954657 1105 22-23 0.5232591953538517 1105 24-25 0.45994102903916456 1105 26-27 0.4043587938747777 1105 28-29 0.44819997174319326 1105 30-31 0.36248076259990825 1105 32-33 0.29563987097061784 1105 34-35 0.3752064346573363 1105 36-37 0.3645655655135016 1105 38-39 0.39927951072836265 1105 40-41 0.5262659083330661 1105 42-43 0.4874480018587235 1105 44-45 0.48500231849341446 1105 46-47 0.6076779251274687 1105 48-49 0.6154742710316228 1105 50-51 0.6458016213213895 1105 52-53 0.5926561841841078 1105 54-55 0.6575226800131659 1105 56-57 0.584062770941884 1105 58-59 0.6376714408558115 1105 60-61 0.6755599703571562 1105 62-63 0.6205736223160372 1105 64-65 0.5523135904244825 1105 66-67 0.399580208619021 1105 68-69 0.42957065965315877 1105 70-71 0.4259976749055383 1105 72-73 0.3795652219060095 1105 74-75 0.3136507913941031 1105 76-77 0.4033981108771094 1105 78-79 0.29019610510867366 1105 80-81 0.3953652975693309 1105 82-83 0.43107838776201746 1105 84-85 0.37544060956784264 1105 86-87 0.3367345656462213 1105 88-89 0.2650532740311853 1105 90-91 0.4345363085184566 1105 92-93 0.3421592207863391 1105 94-95 0.355307432084917 1105 96-97 0.39097861919187693 1105 98-99 0.46155395515969033 1105 100-101 0.49452148934853923 1106 1 0.04707826582948371 1106 2 0.1035979792291215 1106 3 0.1822534459023366 1106 4 0.18158419454334762 1106 5 0.07219880601554962 1106 6 -0.002480055791679092 1106 7 0.12826649076060193 1106 8 0.1493924334977379 1106 9 0.24838678160111272 1106 10-11 0.32169869731793455 1106 12-13 0.22949096687575832 1106 14-15 0.235578003142642 1106 16-17 0.23611823712327862 1106 18-19 0.3685873484273827 1106 20-21 0.3617198270590194 1106 22-23 0.327069557939204 1106 24-25 0.38354847678701987 1106 26-27 0.13211611460299366 1106 28-29 0.284354719744627 1106 30-31 0.28942141554973233 1106 32-33 0.3957159018532863 1106 34-35 0.4078060464193385 1106 36-37 0.5084986402476162 1106 38-39 0.482422870490673 1106 40-41 0.4305498670039256 1106 42-43 0.4756256800960301 1106 44-45 0.46144218513128976 1106 46-47 0.49328683357271785 1106 48-49 0.35540659506677486 1106 50-51 0.3741021812930825 1106 52-53 0.4097362817297494 1106 54-55 0.42568298137810956 1106 56-57 0.40494735210499044 1106 58-59 0.3299934522768382 1106 60-61 0.491922381593227 1106 62-63 0.31621764628865634 1106 64-65 0.3035062860330058 1106 66-67 0.2356981565426679 1106 68-69 0.4577266196264276 1106 70-71 0.41716966976370173 1106 72-73 0.4436376620959912 1106 74-75 0.45777561092674546 1106 76-77 0.2448249929099653 1106 78-79 0.48151790059068844 1106 80-81 0.3486286334992066 1106 82-83 0.3917915630943192 1106 84-85 0.39363968950936723 1106 86-87 0.4662884763469606 1106 88-89 0.49814502323893706 1106 90-91 0.5415102341653402 1106 92-93 0.791034755122336 1106 94-95 0.8168800107698111 1106 96-97 0.8185872133384571 1106 98-99 0.8265883414689306 1106 100-101 1.0100784819851327 1107 1 0.2503024749553653 1107 2 0.21497814995469255 1107 3 0.16457862986012373 1107 4 0.2724893330153222 1107 5 0.21456201060811964 1107 6 0.2652420537251885 1107 7 0.059365263652580325 1107 8 0.1935511528324838 1107 9 0.251390774608538 1107 10-11 0.2357501362454073 1107 12-13 0.2913955807497075 1107 14-15 0.43381775661032407 1107 16-17 0.3646049831131748 1107 18-19 0.4473522796134475 1107 20-21 0.3839378036240291 1107 22-23 0.5477748734602486 1107 24-25 0.38593428046964817 1107 26-27 0.42872247867845203 1107 28-29 0.2918373771445033 1107 30-31 0.3405867599535455 1107 32-33 0.5057193622974694 1107 34-35 0.5841951636321525 1107 36-37 0.5914386725642728 1107 38-39 0.578612155902313 1107 40-41 0.4956259646937724 1107 42-43 0.6564543546994415 1107 44-45 0.6202098643171396 1107 46-47 0.7100669566397428 1107 48-49 0.5576703318021927 1107 50-51 0.6630352205919081 1107 52-53 0.6574734410812155 1107 54-55 0.6132085226201198 1107 56-57 0.4955182280420942 1107 58-59 0.5621845550854374 1107 60-61 0.3524249956224992 1107 62-63 0.3453870445452907 1107 64-65 0.285800217577723 1107 66-67 0.480079083300069 1107 68-69 0.5545155641710409 1107 70-71 0.5047940239450313 1107 72-73 0.37008712688873757 1107 74-75 0.31290303116339757 1107 76-77 0.19424055619129277 1107 78-79 0.2661417576097378 1107 80-81 0.3122145940081076 1107 82-83 0.34561930333355306 1107 84-85 0.18799139940908915 1107 86-87 0.20526743733310937 1107 88-89 0.24581890336817835 1107 90-91 0.42267882439498194 1107 92-93 0.4307862536340572 1107 94-95 0.5280791166299785 1107 96-97 0.4827248868004155 1107 98-99 0.5826722736132481 1107 100-101 0.6851939942226899 1108 1 0.17977532992429346 1108 2 0.155578498213508 1108 3 0.10242756129962771 1108 4 0.14045587175750285 1108 5 0.17381443798065987 1108 6 0.1688546470049559 1108 7 0.15333509126661937 1108 8 0.1639098723248651 1108 9 0.16400062787921854 1108 10-11 0.23699153470472112 1108 12-13 0.254866987151388 1108 14-15 0.08117627377681202 1108 16-17 0.19242495396964188 1108 18-19 0.1648163398635134 1108 20-21 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-0.8663388336749946 2314 12-13 -0.8511628295577083 2314 14-15 -1.1217081687330435 2314 16-17 -1.107917356233898 2314 18-19 -1.2260673740247725 2314 20-21 -1.3747311673897258 2314 22-23 -1.3531050335474575 2314 24-25 -1.3488882947149463 2314 26-27 -1.4936253082947601 2314 28-29 -1.5232140726472352 2314 30-31 -1.5988800113166732 2314 32-33 -1.4588886093751796 2314 34-35 -1.4393971242219763 2314 36-37 -1.5910677748441486 2314 38-39 -1.6112873518024529 2314 40-41 -1.8278724772824333 2314 42-43 -2.0398711260149156 2314 44-45 -1.997776175329264 2314 46-47 -1.9039766450087185 2314 48-49 -1.977979880585842 2314 50-51 -2.0282629599393616 2314 52-53 -1.8423266389059734 2314 54-55 -1.9798136982807009 2314 56-57 -2.2420743110907253 2314 58-59 -2.11899692471588 2314 60-61 -2.2330140275747468 2314 62-63 -1.9102389606893624 2314 64-65 -1.7174234386807008 2314 66-67 -1.789631907840917 2314 68-69 -1.5286259853361344 2314 70-71 -1.484711062530323 2314 72-73 -1.825867190261416 2314 74-75 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2315 32-33 -1.829768065545764 2315 34-35 -1.8790460377328415 2315 36-37 -1.8324159679417207 2315 38-39 -1.772765075268147 2315 40-41 -1.994759918897401 2315 42-43 -1.7913914782003246 2315 44-45 -1.8105034976287797 2315 46-47 -1.8368485869360924 2315 48-49 -1.8853382153309823 2315 50-51 -2.0377357266352654 2315 52-53 -2.002587939096493 2315 54-55 -2.063499107403608 2315 56-57 -1.9994018845587718 2315 58-59 -2.033558892871504 2315 60-61 -2.029450922625511 2315 62-63 -1.8314602666408568 2315 64-65 -1.8844140492067183 2315 66-67 -2.0072060252440984 2315 68-69 -1.9375378061997708 2315 70-71 -1.927111965638133 2315 72-73 -2.00155880908299 2315 74-75 -2.019394666911875 2315 76-77 -1.9379117418656335 2315 78-79 -2.0256383196194356 2315 80-81 -1.893330674760609 2315 82-83 -1.8289380037215714 2315 84-85 -1.8505309656256692 2315 86-87 -1.8436854249968917 2315 88-89 -2.004187487737372 2315 90-91 -1.9527950050693015 2315 92-93 -1.845164593746059 2315 94-95 -1.9035788033747814 2315 96-97 -1.8932768479823388 2315 98-99 -2.0572786644732233 2315 100-101 -2.4070695441035355 2316 1 -1.3118792737225 2316 2 -1.9295315298081128 2316 3 -1.7177971428797107 2316 4 -1.3791997677596228 2316 5 -1.7313516332819106 2316 6 -1.5024610411183943 2316 7 -1.3671990459729173 2316 8 -1.6719478415890023 2316 9 -1.848988920454751 2316 10-11 -1.858547582680636 2316 12-13 -2.224996291245411 2316 14-15 -2.916944883210654 2316 16-17 -3.0250776025052133 2316 18-19 -2.7875309688656884 2316 20-21 -3.0898124533229634 2316 22-23 -2.7809095060279176 2316 24-25 -2.762460226277703 2316 26-27 -2.931896734245562 2316 28-29 -2.838775820960521 2316 30-31 -3.1318519376381673 2316 32-33 -3.0549285883087194 2316 34-35 -3.0255078854465083 2316 36-37 -3.024831595481672 2316 38-39 -3.515095714646016 2316 40-41 -3.2030952517149167 2316 42-43 -3.313038610289045 2316 44-45 -3.215289403155591 2316 46-47 -3.8173602718381936 2316 48-49 -3.5891612930700063 2316 50-51 -3.8063960675042665 2316 52-53 -3.7077006319722976 2316 54-55 -3.454486986398358 2316 56-57 -3.5515286458191895 2316 58-59 -3.3316639368544543 2316 60-61 -3.1381259410298092 2316 62-63 -3.4580977815931746 2316 64-65 -3.5419783336315973 2316 66-67 -3.5306759149318268 2316 68-69 -3.625646628268939 2316 70-71 -3.5545472226185133 2316 72-73 -3.5570004508371262 2316 74-75 -3.6158041538724746 2316 76-77 -3.6351023671376517 2316 78-79 -3.610958597760689 2316 80-81 -3.5013722148477555 2316 82-83 -3.4303002303872177 2316 84-85 -3.6394009381191665 2316 86-87 -3.6897914215549683 2316 88-89 -3.606976574134034 2316 90-91 -3.8313515658240895 2316 92-93 -4.044883582414048 2316 94-95 -4.156327388219854 2316 96-97 -4.294303340737418 2316 98-99 -4.355905521667157 2316 100-101 -4.4648797296136635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22536.0 3 8093.0 4 8179.0 5 8613.0 6 9536.0 7 11368.0 8 12972.0 9 16189.0 10 19011.0 11 22091.0 12 25991.0 13 30008.0 14 31095.0 15 29709.0 16 33406.0 17 37894.0 18 34738.0 19 30955.0 20 32197.0 21 35455.0 22 39235.0 23 43945.0 24 50104.0 25 56832.0 26 64818.0 27 74541.0 28 85612.0 29 100366.0 30 117403.0 31 140580.0 32 171325.0 33 213076.0 34 275569.0 35 376408.0 36 540861.0 37 749995.0 38 631173.0 39 101868.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.821006260143754 18.535511702256198 13.91238691131253 23.731095126287517 2 15.265940819365003 19.93625467174746 40.37779118917695 24.420013319710584 3 19.825423679034664 23.74448679978686 29.33043491638303 27.099654604795443 4 12.81066711675598 15.961999468938126 37.25643830928363 33.970895105022265 5 13.897079140565705 37.18395246972522 34.389706748184445 14.529261641524634 6 32.67862480688533 35.48689825748886 17.361809577928394 14.472667357697407 7 28.71121681676756 32.14159732451197 20.80875078809376 18.338435070626716 8 26.060739929899512 35.02083838652848 19.822365191238532 19.096056492333474 9 26.12161093503965 16.855467776126574 20.359157820765 36.66376346806878 10-11 25.14696349992847 26.143624172332792 27.436343776459836 21.273068551278904 12-13 26.011410114619586 23.417845322334166 27.745727032190022 22.82501753085623 14-15 22.764205194485346 25.06955412546456 26.14468539609765 26.021555283952434 16-17 22.373451373621 28.117372202500167 26.74546374678811 22.763712677090723 18-19 22.26618562968222 27.179238159792373 28.53745269503312 22.017123515492283 20-21 23.143500393093667 26.23470325039764 28.43431514321705 22.187481213291647 22-23 22.573839195379144 26.221885035752784 28.435985989024402 22.768289779843673 24-25 22.61066396045475 26.211164242947472 28.670983615422053 22.50718818117573 26-27 22.425682041413594 26.386668755107472 28.679070513313576 22.508578690165354 28-29 22.202800564238007 27.021337216111196 28.43806171076451 22.337800508886282 30-31 22.478113677793488 26.466396696034856 28.35518906974319 22.700300556428466 32-33 22.285679999906797 26.549885766679754 28.40955295800804 22.75488127540541 34-35 22.85441368346751 26.57696786969425 28.19207876944465 22.376539677393588 36-37 22.841320880204638 26.249361676738474 28.46557324499856 22.443744198058326 38-39 22.50408582400073 26.599442341897355 28.388935589775667 22.50753624432625 40-41 22.73241146828373 26.606066516569506 28.255673604859986 22.405848410286776 42-43 22.45855534157922 26.650288587325736 27.946615555642897 22.944540515452147 44-45 22.645470881257832 26.653552366171606 28.043047441816505 22.657929310754056 46-47 22.89524745806252 26.87341953248167 27.677572401891844 22.553760607563973 48-49 22.711613473652584 26.81365720062791 27.7514861506519 22.723243175067605 50-51 22.535530261774834 27.040937670178796 27.84931437138183 22.57421769666454 52-53 22.813691994128266 27.052144227903984 27.497894386227184 22.636269391740566 54-55 22.457704744972055 26.81076615425002 27.751955341769808 22.97957375900812 56-57 22.618338484023425 27.02083640595009 27.550357678906412 22.81046743112007 58-59 22.825328783928324 26.820573728788087 27.552833978484614 22.801263508798968 60-61 22.630724407675988 26.863606697580504 27.963496308821057 22.542172585922447 62-63 22.465355641840567 26.859079958883875 27.965105099232186 22.71045930004337 64-65 22.279965351002463 27.083833794801222 27.750568405267366 22.885632448928945 66-67 22.366227085327893 26.939964395713538 27.56181078711122 23.13199773184735 68-69 22.43019089783114 26.75668413903122 27.780439408592134 23.032685554545516 70-71 22.511353611173295 26.61686200685997 27.577646249455416 23.294138132511318 72-73 22.431604163524472 26.583509598345113 27.82435989678361 23.160526341346806 74-75 22.27459206281011 27.126653113220883 27.47081282298245 23.127942000986558 76-77 22.370571333717184 26.66352301831419 27.599820973476607 23.36608467449202 78-79 22.425330490963688 26.747757938262197 27.647284194394505 23.179627376379607 80-81 22.548627915598942 26.70930149600065 27.51139456359143 23.23067602480898 82-83 22.491756206663137 26.636748714113285 27.71146261287221 23.160032466351367 84-85 22.41360393798451 26.719265609494432 27.593028146707795 23.274102305813262 86-87 22.374877009277398 26.643776637266654 27.634709569932838 23.34663678352311 88-89 22.369369008902513 26.584298472273797 27.41572539442647 23.63060712439722 90-91 22.326700498705705 26.53162320663381 27.57921523319245 23.56246106146804 92-93 22.47422620102715 26.460277373637748 27.651742638167626 23.413753787167476 94-95 22.30452449551538 26.33283114203823 27.759922607313552 23.602721755132837 96-97 22.18786886565591 26.036275553234127 28.040997501659167 23.734858079450795 98-99 22.274430372164026 26.155518718649763 27.59842536267764 23.97162554650857 100-101 22.416220960778077 25.658152952058895 27.958162626778897 23.967463460384128 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2769.0 1 1609.0 2 582.0 3 746.0 4 650.0 5 636.0 6 1134.0 7 2163.5 8 3361.5 9 3857.0 10 3548.0 11 3319.5 12 3506.0 13 3876.5 14 4255.0 15 4555.0 16 4925.0 17 5296.5 18 5593.5 19 5938.5 20 6557.5 21 7534.5 22 8874.0 23 10886.0 24 13494.5 25 16932.5 26 22106.5 27 28507.0 28 34335.5 29 40932.0 30 48822.0 31 56817.0 32 65573.5 33 72403.5 34 77675.5 35 84399.0 36 94078.5 37 103183.0 38 109309.5 39 114602.5 40 120496.5 41 126994.0 42 131675.0 43 136220.5 44 142742.0 45 150096.0 46 155646.0 47 164803.5 48 181619.0 49 181897.5 50 166915.5 51 159355.0 52 155388.0 53 152652.5 54 149563.0 55 140661.0 56 127775.5 57 114846.0 58 104602.5 59 93607.5 60 79800.5 61 66736.5 62 54018.5 63 41526.5 64 30331.0 65 21955.0 66 16130.5 67 12364.5 68 9303.5 69 6793.0 70 5116.5 71 3747.5 72 2775.0 73 2201.0 74 1686.5 75 1220.5 76 810.5 77 502.0 78 350.0 79 229.5 80 149.5 81 98.5 82 87.5 83 75.0 84 51.0 85 41.0 86 32.0 87 25.5 88 20.5 89 17.0 90 16.0 91 14.0 92 11.0 93 10.0 94 11.5 95 10.0 96 5.5 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15552657319856536 2 0.09122455633704411 3 0.08481997298430294 4 0.09970189575667242 5 0.12028972006148399 6 0.038683683450556616 7 0.04187433042992221 8 0.045670501653546976 9 0.046159578927756295 10-11 0.04466905771111836 12-13 0.0484768736317481 14-15 0.04654385392892077 16-17 0.04499510922725791 18-19 0.04889608272392752 20-21 0.051283245609949235 22-23 0.06370813731426708 24-25 0.07974288509012996 26-27 0.08193208812706693 28-29 0.07120732218547673 30-31 0.06037775397084168 32-33 0.04995575015138106 34-35 0.29218873724905675 36-37 0.4636336112534352 38-39 0.4415319763379757 40-41 0.4169500209604546 42-43 0.44678373468722343 44-45 0.4561344263822255 46-47 0.4553192975918767 48-49 0.47183147794494384 50-51 0.46118822488238853 52-53 0.4682449112674088 54-55 0.4597093483627556 56-57 0.43799198844846055 58-59 0.41756718990171876 60-61 0.3876170292049001 62-63 0.4443150589221669 64-65 0.481170524942941 66-67 0.4825329544925241 68-69 0.4925241045228003 70-71 0.4900204946667288 72-73 0.4807280264567516 74-75 0.473892589314826 76-77 0.4558433089571009 78-79 0.4307140528203456 80-81 0.43632679677674785 82-83 0.4476337975685873 84-85 0.4454329498346453 86-87 0.45636732032232524 88-89 0.4672551120219852 90-91 0.47131911127672454 92-93 0.4732987097675718 94-95 0.4780846802366202 96-97 0.47892309842097913 98-99 0.4729610135544273 100-101 0.46053612185010945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4293800.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.202160274111094 #Duplication Level Percentage of deduplicated Percentage of total 1 70.3118164118453 34.594932602594874 2 14.481496510575806 14.250418246446628 3 6.016481179265499 8.88071613805182 4 3.0512049121180698 6.005034924607533 5 1.7141340257760638 4.216954853377058 6 1.0789095254118883 3.185080763434852 7 0.7060523866652055 2.4317511883434073 8 0.5013539092413526 1.9734156317236142 9 0.36352995867284454 1.6097813361956055 >10 1.647108015536742 13.932497318005312 >50 0.088341179963437 2.9850492688500974 >100 0.03539553823436782 3.083705072427243 >500 0.002610279308083754 0.9123531515422556 >1k 0.0013763289143668097 1.2075350403162135 >5k 1.8983847094711822E-4 0.7307744640836303 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9664 0.2250687037123294 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8734 0.2034095672830593 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7737 0.18019004145512135 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5160 0.12017327309143416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4951 0.11530578974335089 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5618333410964647E-4 2 0.0 0.0 0.0 0.0 2.5618333410964647E-4 3 0.0 0.0 0.0 0.0 2.5618333410964647E-4 4 0.0 0.0 0.0 2.328939400996786E-5 2.5618333410964647E-4 5 0.0 0.0 0.0 2.328939400996786E-5 2.794727281196143E-4 6 0.0 0.0 0.0 2.328939400996786E-5 4.4249848618938936E-4 7 0.0 0.0 0.0 2.328939400996786E-5 4.4249848618938936E-4 8 0.0 0.0 0.0 2.328939400996786E-5 4.657878801993572E-4 9 0.0 0.0 0.0 3.2605151613955004E-4 4.89077274209325E-4 10-11 0.0 0.0 0.0 3.493409101495179E-4 5.123666682192929E-4 12-13 0.0 0.0 0.0 3.842750011644697E-4 0.0013857189435930875 14-15 0.0 0.0 0.0 4.192090921794215E-4 0.0015836787926778145 16-17 1.164469700498393E-5 0.0 0.0 4.4249848618938936E-4 0.0017816386417625414 18-19 2.328939400996786E-5 0.0 0.0 4.4249848618938936E-4 0.0018747962178024128 20-21 3.493409101495179E-5 0.0 0.0 5.123666682192929E-4 0.0020727560668871393 22-23 6.986818202990358E-5 0.0 0.0 6.986818202990358E-4 0.0022241371279519305 24-25 6.986818202990358E-5 0.0 0.0 0.0010247333364385859 0.0022590712189668825 26-27 6.986818202990358E-5 0.0 0.0 0.0015953234896827985 0.0024337416740416416 28-29 1.0480227304485537E-4 0.0 0.0 0.0039941310727094875 0.002491965159066561 30-31 1.3973636405980715E-4 0.0 0.0 0.012389957613302902 0.0025036098560715448 32-33 1.3973636405980715E-4 0.0 0.0 0.0292049000884997 0.0025618333410964648 34-35 1.7467045507475894E-4 0.0 0.0 0.05490474637849923 0.0026317015231263684 36-37 2.0960454608971076E-4 0.0 0.0 0.09761749499278029 0.0026317015231263684 38-39 2.0960454608971076E-4 0.0 0.0 0.15939261260422005 0.002783082584191159 40-41 2.0960454608971076E-4 0.0 0.0 0.22797987796357538 0.0028645954632260467 42-43 2.2124924309469468E-4 0.0 0.0 0.3006311425776701 0.0029577530392659182 44-45 2.328939400996786E-4 0.0 0.0 0.3906446504261959 0.0031557128883506454 46-47 2.5618333410964647E-4 0.0 0.0 0.502852950766221 0.0033886068284503237 48-49 2.5618333410964647E-4 0.0 0.0 0.6243420746192184 0.0034701197074852113 50-51 2.5618333410964647E-4 0.0 0.0 0.7542386697098142 0.0036331454655549864 52-53 2.5618333410964647E-4 0.0 0.0 0.8808631049420095 0.0038660394056546647 54-55 2.794727281196143E-4 0.0 0.0 1.0234757091620477 0.003947552284689552 56-57 2.794727281196143E-4 0.0 0.0 1.1938376263449624 0.003994131072709488 58-59 2.794727281196143E-4 0.0 0.0 1.383343425404071 0.004005775769714472 60-61 2.794727281196143E-4 0.0 0.0 1.592831524523732 0.0040290651637244395 62-63 2.794727281196143E-4 0.0 0.0 1.7953211607433974 0.004261959103824119 64-65 3.027621221295822E-4 0.0 0.0 2.0083026689645536 0.004366761376868974 66-67 3.027621221295822E-4 0.0 0.0 2.256532675019796 0.004401695467883926 68-69 3.027621221295822E-4 0.0 0.0 2.5415715683077926 0.004401695467883926 70-71 3.027621221295822E-4 0.0 0.0 2.846138618473147 0.004471563649913829 72-73 3.2605151613955004E-4 0.0 0.0 3.156411570170944 0.004902417439098234 74-75 3.493409101495179E-4 0.0 0.0 3.476722250687037 0.005275047743257721 76-77 3.493409101495179E-4 0.0 0.0 3.817387861567842 0.005414784107317528 78-79 3.493409101495179E-4 0.0 0.0 4.216905771111836 0.005496296986352415 80-81 3.959196981694536E-4 0.0 0.0 4.6420536587638 0.00565932274442219 82-83 3.959196981694536E-4 0.0 0.0 5.069623643392799 0.006253202291676371 84-85 3.959196981694536E-4 0.0 0.0 5.505368205319297 0.006323070473706274 86-87 3.959196981694536E-4 0.0 0.0 5.959918952908845 0.006532675019795985 88-89 3.959196981694536E-4 0.0 0.0 6.458591457450277 0.00669570077786576 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14730 0.0 23.36425 1 TACATGG 14185 0.0 22.91449 2 ACATGGG 14540 0.0 21.801035 3 GTACCGT 380 0.0 21.18101 6 GTATCAA 23925 0.0 20.665712 1 GAGTACT 11400 0.0 20.226965 12-13 GTATAGC 1565 0.0 18.779991 1 TAGCCCT 3075 0.0 18.336535 4 GTACTTT 12535 0.0 18.16927 14-15 TCAACGC 26775 0.0 18.15655 4 CAACGCA 26775 0.0 18.121155 5 ATCAACG 26855 0.0 18.10246 3 AGAGTAC 18635 0.0 18.090357 10-11 TATCAAC 26940 0.0 18.062933 2 AGTACTT 12255 0.0 18.02374 12-13 CATGGGG 7740 0.0 17.875463 4 AACGCAG 27305 0.0 17.773092 6 GGTATCA 18800 0.0 17.499258 1 GTGTAGC 2910 0.0 17.430458 1 GAGTACA 12400 0.0 17.39433 1 >>END_MODULE