FastQCFastQC Report
Thu 26 May 2016
SRR938767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4293800
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99640.23205552191531975No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99280.23121710373096094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84480.19674880059620847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56930.13258652009874702No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45620.10624621547347338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG153200.022.2302591
TACATGG150500.022.1035772
ACATGGG150950.020.8744953
GAGTACT125700.020.55707412-13
GTATCAA255150.020.5163151
AGAGTAC208550.018.79580910-11
GGACCGT4550.018.7733656
GTACTTT137300.018.54387314-15
AGTACTT135700.018.51760512-13
CATGGGG84700.018.1527584
CAACGCA289700.017.8713465
TACCTGG27900.017.859372
TCAACGC290350.017.8149934
ATCAACG290550.017.8027323
TATCAAC294850.017.6557642
GGTATCA201000.017.5597931
AACGCAG296950.017.4989436
GTGTAGC29100.017.023061
CGCGGAA2700.016.79674582-83
TACTTTT157900.015.92923714-15
Produced by FastQC (version 0.11.5)